FvH4_7g15740

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006578
Physical Location & Seq
Forward (+)
13567840 .. 13573118
5279 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g15740.t3

Sequence Viewer

Length: 1257 bp
ATGGATGATCTGAAGGAGGAGCTCTCACCTGAGGACTTCAAGGCTACTAAAAGTAACCGAATCAGATATTCTAGACCAGATTTTAGACAGCTTCCTTATTTCGCGACTGATTTTGAATGGAAAGATTATAGCCCTGCTGTTTTCAGACGTATACTGGAGAATGACAGTATTGAATATTCCGATTACCAGTTGTCAGTATGTGGAGATGAGACTCTAAGGGAGGTTTCATTCTCTATGAAACCTGGTATGATGTTCTTATTGTCCAACGACAATCGGTTTGTCATCAAAACCCTGCGCAAATCTGAGGTGAAGATACTCCTAGAGATGCTTCCCAACTACTACCATCATCTTATGAAGTTCGGTTCCTCAGTAATAGTCAAACTTTATGGAGTTCATGTTGTCAGGCCAGCAGGAGGTATTAAGGTTTACTTTGGTGTTTGGGAAAACATAGTCCCGTCAGATATTTCCATATTTGAGCATTATGATCTCAAAGGCTCTGCAAAAGGTCGAGCAGAGAACAAAATCATTGTTCATGAGAGACCTGTACATAAGGATATGGATTTTGATTTTTGCTTTTACCTGGATCCATTGGTTCGAGCTCGACTCTTAGCGCAAATCAAGTATGACTGCGAGTTTTTGGAAGCTGAAGGCATCATGGATTATAGTTTGTTTCTAGCTATACAAATCGAATCTCCACATGAAGTTGATAAAAGAAATTCCAGAAAGACTGATAAAGCGATAATGGAAAGAACTTCCAGAACGACTGATAAAGATATACTGGAAAGAATTTCCCGAAGGACTGATAAAGAGATAATGGACGATACTTCAGAAGGAAGTGAATGCTCAGAGTTAACTCTTGCTGATATTTGCGATCTACTTGACAGGCCTGGTTATAAATTTGGTGCGAGCCTGCCAGCACGTGCTGTCCGGGACTTCAGGAAAGAAATGGTGGGATGTTCGAGATCATATAAGTCCAGAAACTCTAGATCTAGAGCACAGCCAGCACATGAATGCTTCAATGTTCTTTTGTTCTTTAGAATCATAGATATTTGTCAGAGTTACAATATGAGAAAACGCCTTGAGCATGCTTGCAAGTCCATCAAATATGACTCAAAGTCCATCGCATCTGTGAACCCCAAAGCTTATTCTTCTCGCTTTCAAGAGTTTATGAGCGAAGTGTTTCTACCTGAAGAGTCAAACGACTTTAGCAATAGCCGGGTCTCAGCTGCATCATCATCGAAGATTTCTGTTAGATGA

Protein Analysis

419

Amino Acids

48.58

Weight (kDa)

7.56

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 67 - 392 9.5e-72 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 894
Acc16I TGCGCA 1 cut(s) 296
AccI GTMKAC 1 cut(s) 151
AccII CGCG 1 cut(s) 104
AclWI GGATC 2 cut(s) 578, 591
AcsI RAATTY 3 cut(s) 715, 786, 896
AcuI CTGAAG 5 cut(s) 32, 666, 810, 919, 1209
AcvI CACGTG 1 cut(s) 920
AfaI GTAC 1 cut(s) 546
AfiI CCNNNNNNNGG 1 cut(s) 413
AgsI TTSAA 5 cut(s) 40, 116, 173, 1018, 1160
AhdI GACNNNNNGTC 1 cut(s) 1114
AjnI CCWGG 3 cut(s) 241, 579, 886
AluBI AGCT 7 cut(s) 22, 91, 599, 644, 677, 1142, 1226
AluI AGCT 7 cut(s) 22, 91, 599, 644, 677, 1142, 1226
Alw21I GWGCWC 3 cut(s) 24, 601, 997
Alw26I GTCTC 3 cut(s) 203, 532, 1225
AlwI GGATC 2 cut(s) 578, 591
AoxI GGCC 2 cut(s) 404, 884
ApeKI GCWGC 1 cut(s) 1226
ApoI RAATTY 3 cut(s) 715, 786, 896
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 1179
AspLEI GCGC 2 cut(s) 297, 613
AsuC2I CCSGG 2 cut(s) 929, 1217
AsuHPI GGTGA 2 cut(s) 18, 319
AxyI CCTNAGG 1 cut(s) 30
BamHI GGATCC 1 cut(s) 583
BanII GRGCYC 2 cut(s) 24, 601
BarI GAAGNNNNNNTAC 2 cut(s) 1167, 1199
BbrPI CACGTG 1 cut(s) 920
Bbv12I GWGCWC 3 cut(s) 24, 601, 997
BbvI GCAGC 1 cut(s) 1213
BccI CCATC 3 cut(s) 351, 1106, 1127
BcgI CGANNNNNNTGC 2 cut(s) 1218, 1252
BciT130I CCWGG 3 cut(s) 243, 581, 888
BcnI CCSGG 2 cut(s) 929, 1217
BcoDI GTCTC 3 cut(s) 203, 532, 1225
BfaI CTAG 5 cut(s) 72, 320, 674, 984, 990
BglII AGATCT 1 cut(s) 986
BisI GCNGC 1 cut(s) 1227
BlsI GCNGC 1 cut(s) 1228
Bme1390I CCNGG 5 cut(s) 243, 581, 888, 929, 1217
BmeRI GACNNNNNGTC 1 cut(s) 1114
BmiI GGNNCC 2 cut(s) 364, 585
BmrFI CCNGG 5 cut(s) 243, 581, 888, 929, 1217
BmsI GCATC 4 cut(s) 315, 660, 1133, 1238
BplI GAGNNNNNCTC 4 cut(s) 8, 40, 588, 620
BpmI CTGGAG 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 1100
BpuMI CCSGG 2 cut(s) 929, 1217
BsaAI YACGTR 1 cut(s) 920
BsaI GGTCTC 2 cut(s) 532, 1225
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse1I ACTGG 3 cut(s) 159, 187, 783
Bse21I CCTNAGG 1 cut(s) 30
BseBI CCWGG 3 cut(s) 243, 581, 888
BseGI GGATG 2 cut(s) 10, 959
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMII CTCAG 5 cut(s) 21, 294, 381, 858, 1236
BseNI ACTGG 3 cut(s) 159, 187, 783
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 1213
Bsh1236I CGCG 1 cut(s) 104
BshFI GGCC 2 cut(s) 406, 886
BsiHKAI GWGCWC 3 cut(s) 24, 601, 997
BsiSI CCGG 2 cut(s) 928, 1216
BslFI GGGAC 2 cut(s) 437, 944
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 3 cut(s) 203, 532, 1225
BsmFI GGGAC 2 cut(s) 437, 944
BsmI GAATGC 2 cut(s) 845, 1016
BsnI GGCC 2 cut(s) 406, 886
Bso31I GGTCTC 2 cut(s) 532, 1225
Bsp1286I GDGCHC 3 cut(s) 24, 601, 997
Bsp1407I TGTACA 1 cut(s) 544
Bsp143I GATC 6 cut(s) 7, 484, 583, 871, 962, 986
Bsp68I TCGCGA 1 cut(s) 104
BspANI GGCC 2 cut(s) 406, 886
BspCNI CTCAG 5 cut(s) 22, 295, 380, 857, 1235
BspFNI CGCG 1 cut(s) 104
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 2 cut(s) 364, 585
BspPI GGATC 2 cut(s) 578, 591
BspTNI GGTCTC 2 cut(s) 532, 1225
BsrGI TGTACA 1 cut(s) 544
BsrI ACTGG 3 cut(s) 159, 187, 783
BssMI GATC 6 cut(s) 7, 484, 583, 871, 962, 986
BssNAI GTATAC 1 cut(s) 152
Bst1107I GTATAC 1 cut(s) 152
Bst2UI CCWGG 3 cut(s) 243, 581, 888
Bst4CI ACNGT 1 cut(s) 167
Bst6I CTCTTC 1 cut(s) 1185
BstAUI TGTACA 1 cut(s) 544
BstBAI YACGTR 1 cut(s) 920
BstC8I GCNNGC 7 cut(s) 408, 907, 911, 915, 1002, 1086, 1090
BstDEI CTNAG 7 cut(s) 30, 215, 303, 367, 607, 844, 1222
BstF5I GGATG 2 cut(s) 10, 959
BstFNI CGCG 1 cut(s) 104
BstHHI GCGC 2 cut(s) 297, 613
BstKTI GATC 6 cut(s) 10, 487, 586, 874, 965, 989
BstMAI GTCTC 3 cut(s) 203, 532, 1225
BstMBI GATC 6 cut(s) 7, 484, 583, 871, 962, 986
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 3 cut(s) 243, 581, 888
BstNSI RCATGY 1 cut(s) 1088
BstSCI CCNGG 5 cut(s) 241, 579, 886, 927, 1215
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 1 cut(s) 1213
BstX2I RGATCY 2 cut(s) 583, 986
BstYI RGATCY 2 cut(s) 583, 986
BstZ17I GTATAC 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 30
BsuRI GGCC 2 cut(s) 406, 886
BtgZI GCGATG 1 cut(s) 1105
BtsCI GGATG 2 cut(s) 10, 959
BtuMI TCGCGA 1 cut(s) 104
Cac8I GCNNGC 7 cut(s) 408, 907, 911, 915, 1002, 1086, 1090
CciI TCATGA 1 cut(s) 532
CfoI GCGC 2 cut(s) 297, 613
CsiI ACCWGGT 1 cut(s) 241
Csp6I GTAC 1 cut(s) 545
CviAII CATG 6 cut(s) 395, 533, 655, 698, 1007, 1085
CviQI GTAC 1 cut(s) 545
DdeI CTNAG 7 cut(s) 30, 215, 303, 367, 607, 844, 1222
DpnI GATC 6 cut(s) 9, 486, 585, 873, 964, 988
DpnII GATC 6 cut(s) 7, 484, 583, 871, 962, 986
DriI GACNNNNNGTC 1 cut(s) 1114
Eam1104I CTCTTC 1 cut(s) 1185
Eam1105I GACNNNNNGTC 1 cut(s) 1114
EarI CTCTTC 1 cut(s) 1185
Ecl136II GAGCTC 2 cut(s) 22, 599
Eco147I AGGCCT 1 cut(s) 886
Eco24I GRGCYC 2 cut(s) 24, 601
Eco31I GGTCTC 2 cut(s) 532, 1225
Eco53kI GAGCTC 2 cut(s) 22, 599
Eco57I CTGAAG 5 cut(s) 32, 666, 810, 919, 1209
Eco72I CACGTG 1 cut(s) 920
Eco81I CCTNAGG 1 cut(s) 30
EcoICRI GAGCTC 2 cut(s) 22, 599
EcoRII CCWGG 3 cut(s) 241, 579, 886
EcoT38I GRGCYC 2 cut(s) 24, 601
FaeI CATG 6 cut(s) 398, 536, 658, 701, 1010, 1088
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FaqI GGGAC 2 cut(s) 437, 944
FatI CATG 6 cut(s) 394, 532, 654, 697, 1006, 1084
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 1227
FokI GGATG 2 cut(s) 17, 966
FriOI GRGCYC 2 cut(s) 24, 601
Fsp4HI GCNGC 1 cut(s) 1227
FspBI CTAG 5 cut(s) 72, 320, 674, 984, 990
FspI TGCGCA 1 cut(s) 296
GlaI GCGC 2 cut(s) 296, 612
GluI GCNGC 1 cut(s) 1227
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 2 cut(s) 406, 886
HapII CCGG 2 cut(s) 928, 1216
HhaI GCGC 2 cut(s) 297, 613
Hin1II CATG 6 cut(s) 398, 536, 658, 701, 1010, 1088
Hin6I GCGC 2 cut(s) 295, 611
HinP1I GCGC 2 cut(s) 295, 611
HincII GTYRAC 1 cut(s) 852
HindII GTYRAC 1 cut(s) 852
HindIII AAGCTT 1 cut(s) 1140
HinfI GANTC 7 cut(s) 60, 211, 603, 689, 1038, 1109, 1193
HpaI GTTAAC 1 cut(s) 852
HpaII CCGG 2 cut(s) 928, 1216
HphI GGTGA 2 cut(s) 18, 319
Hpy166II GTNNAC 4 cut(s) 152, 427, 852, 1132
Hpy188I TCNGA 9 cut(s) 12, 65, 146, 181, 304, 460, 829, 847, 1056
Hpy8I GTNNAC 4 cut(s) 152, 427, 852, 1132
HpyAV CCTTC 4 cut(s) 7, 641, 789, 824
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 2 cut(s) 148, 919
HpyCH4V TGCA 3 cut(s) 500, 1092, 1229
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 7 cut(s) 30, 215, 303, 367, 607, 844, 1222
HpySE526I ACGT 2 cut(s) 148, 919
Hsp92II CATG 6 cut(s) 398, 536, 658, 701, 1010, 1088
HspAI GCGC 2 cut(s) 295, 611
KspAI GTTAAC 1 cut(s) 852
Kzo9I GATC 6 cut(s) 7, 484, 583, 871, 962, 986
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 1 cut(s) 1213
LweI GCATC 4 cut(s) 315, 660, 1133, 1238
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 5 cut(s) 72, 320, 674, 984, 990
MaeII ACGT 2 cut(s) 148, 919
MaeIII GTNAC 2 cut(s) 53, 1058
MalI GATC 6 cut(s) 9, 486, 585, 873, 964, 988
MboI GATC 6 cut(s) 7, 484, 583, 871, 962, 986
MboII GAAGA 4 cut(s) 322, 1140, 1202, 1252
MflI RGATCY 2 cut(s) 583, 986
MhlI GDGCHC 3 cut(s) 24, 601, 997
MluCI AATT 3 cut(s) 715, 786, 896
MlyI GAGTC 4 cut(s) 205, 597, 1103, 1202
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 6 cut(s) 10, 25, 214, 298, 376, 407
MroXI GAANNNNTTC 1 cut(s) 1179
MseI TTAA 2 cut(s) 420, 851
MslI CAYNNNNRTG 1 cut(s) 1009
MspA1I CMGCKG 1 cut(s) 1226
MspI CCGG 2 cut(s) 928, 1216
MspR9I CCNGG 5 cut(s) 243, 581, 888, 929, 1217
Mva1269I GAATGC 2 cut(s) 845, 1016
MvaI CCWGG 3 cut(s) 243, 581, 888
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 1 cut(s) 1001
NciI CCSGG 2 cut(s) 929, 1217
NdeII GATC 6 cut(s) 7, 484, 583, 871, 962, 986
NlaIII CATG 6 cut(s) 398, 536, 658, 701, 1010, 1088
NlaIV GGNNCC 2 cut(s) 364, 585
NruI TCGCGA 1 cut(s) 104
NsbI TGCGCA 1 cut(s) 296
NspI RCATGY 1 cut(s) 1088
PaeI GCATGC 1 cut(s) 1088
PagI TCATGA 1 cut(s) 532
PceI AGGCCT 1 cut(s) 886
PctI GAATGC 2 cut(s) 845, 1016
PdmI GAANNNNTTC 1 cut(s) 1179
PfeI GAWTC 3 cut(s) 60, 689, 1038
PfoI TCCNGGA 1 cut(s) 927
PkrI GCNGC 1 cut(s) 1228
PleI GAGTC 4 cut(s) 205, 597, 1103, 1201
PmaCI CACGTG 1 cut(s) 920
PmlI CACGTG 1 cut(s) 920
PpsI GAGTC 4 cut(s) 205, 597, 1103, 1201
Ppu21I YACGTR 1 cut(s) 920
PsiI TTATAA 1 cut(s) 894
Psp124BI GAGCTC 2 cut(s) 24, 601
Psp6I CCWGG 3 cut(s) 241, 579, 886
PspCI CACGTG 1 cut(s) 920
PspGI CCWGG 3 cut(s) 241, 579, 886
PspN4I GGNNCC 2 cut(s) 364, 585
PsuI RGATCY 2 cut(s) 583, 986
PvuII CAGCTG 1 cut(s) 1226
RruI TCGCGA 1 cut(s) 104
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
RseI CAYNNNNRTG 1 cut(s) 1009
SacI GAGCTC 2 cut(s) 24, 601
SaqAI TTAA 2 cut(s) 420, 851
SatI GCNGC 1 cut(s) 1227
Sau3AI GATC 6 cut(s) 7, 484, 583, 871, 962, 986
SchI GAGTC 4 cut(s) 205, 597, 1103, 1202
ScrFI CCNGG 5 cut(s) 243, 581, 888, 929, 1217
SduI GDGCHC 3 cut(s) 24, 601, 997
SexAI ACCWGGT 1 cut(s) 241
SfaNI GCATC 4 cut(s) 315, 660, 1133, 1238
SmiMI CAYNNNNRTG 1 cut(s) 1009
SmlI CTYRAG 1 cut(s) 1079
SmoI CTYRAG 1 cut(s) 1079
SphI GCATGC 1 cut(s) 1088
Sse9I AATT 3 cut(s) 715, 786, 896
SseBI AGGCCT 1 cut(s) 886
SspI AATATT 1 cut(s) 176
SspMI CTAG 5 cut(s) 72, 320, 674, 984, 990
SstI GAGCTC 2 cut(s) 24, 601
StuI AGGCCT 1 cut(s) 886
StyD4I CCNGG 5 cut(s) 241, 579, 886, 927, 1215
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 2 cut(s) 151, 922
TaqI TCGA 6 cut(s) 508, 595, 601, 687, 959, 1238
TasI AATT 3 cut(s) 715, 786, 896
TatI WGTACW 1 cut(s) 544
TfiI GAWTC 3 cut(s) 60, 689, 1038
Tru1I TTAA 2 cut(s) 420, 851
Tru9I TTAA 2 cut(s) 420, 851
TseI GCWGC 1 cut(s) 1226
TspDTI ATGAA 7 cut(s) 216, 251, 368, 383, 521, 714, 1023
XapI RAATTY 3 cut(s) 715, 786, 896
XbaI TCTAGA 3 cut(s) 71, 983, 989
XceI RCATGY 1 cut(s) 1088
XmiI GTMKAC 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 1179
XspI CTAG 5 cut(s) 72, 320, 674, 984, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.