Rw1G024600

phosphatidylinositol 4-phosphate 5-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006578
Physical Location & Seq
Forward (+)
50935329 .. 50940765
5437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G024600.1

Sequence Viewer

Length: 1185 bp
ATGGAGATTCTGAAGGAGGAGCTTTCACCGGCTGACTTTAAGGCTACTAAAAGTAACCGAATCAGATATTCTAGAAATGACTTTAGGCAGCTTCCTTATTTCGCAACTGACTTTGAATGGAAAGATTATAGCCCTGCAGTTTTCAGACGTATACTGGAGCTTGACAGTATTGAATATTCCGATTACCAGTTGTCAGTATGTGGAGATGAGACTCTAAAGGAGGTTACGCTTTCGGGGAAACTTGGTATGGTGTTTTTATTGTCCAACGATAAACGGTTTATTCTCAAAACCCTGCGCAAATCTGAGGTGAAGATCTACCTAGAGATGCTTCCCAACTACTACGACCATCTTATGAAGTATGGATCCTCACTACTAGCCAAACTGTATGGACTTCATGTTGTCAGGCCAGCAGGAGGTGTTAAGGTTTACTTTGTTGTTTTTGGAAACGTAATCCCGTCAGATATTTGCATATTTGAGCATTATGACCTCAAAGGCTCTTCAAAAGGTCGAGCAGAGAACAAAGTCATTATTCAGGAGAGACCCGTACATAAGGATATGGATTTTGATTTTAGCTTTTACCTGGATCCATTGGTTCGAGACCGGCTCTTAGCGCAAATCAAGACTGACTGCGAGTTTTTGGAATCTGAAGGCGTCATGGATTATAGTTTGTTTCTTGCTATACAAATAGAATCTCCACATCAAGTTTCAGTTGATACAAGAACTTCCAGGACTGATAAAGAGATAATAGACGATACTTCAGAGGGAAGTGATGAAAGCTCAGAGTTAACGCTTGCTGATATTTGCGACCTACTTGACAGGCCTGGTTTTAGATTTGGTGCAAGCCTGCCAGCACGTGCTGTCCGGGCCTTCAAGAAAGAAATGGGAAGTTCGAGATCAAATAGGTCCAGAAATTCCAGAGCACAGTCATCAGCACAGGAATGCTTCAATGTTCTTTTATTCTTTGGAATAGTAGATATTTGTCAGAATTACAATATGAGAAAACGCCTTGAGCATGCTTGCAAGTCCATTAAATATGACTCAAAGTCCATTGCAACTGTGAACCCCAAAGTTTATTCTTCTCGCTTTCAAGAGTTTATGAGCGAAGTGTTTCTACCAGAAGTGTCGGACGACTTTTCTGATAGCCGGGTCTCAGCCGCATCATCATCGAAGATTTCTGTTAGATGA

Protein Analysis

394

Amino Acids

45.18

Weight (kDa)

6.16

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIP5K PF01504 67 - 368 4.3e-78 Phosphatidylinositol-4-phosphate 5-Kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 296
AccI GTMKAC 1 cut(s) 151
AciI CCGC 1 cut(s) 1155
AclWI GGATC 4 cut(s) 357, 370, 578, 591
AcsI RAATTY 1 cut(s) 910
AcuI CTGAAG 3 cut(s) 32, 666, 741
AcvI CACGTG 1 cut(s) 854
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 1 cut(s) 546
AfiI CCNNNNNNNGG 1 cut(s) 413
AgsI TTSAA 6 cut(s) 116, 173, 501, 871, 946, 1088
AhdI GACNNNNNGTC 1 cut(s) 1042
AjnI CCWGG 3 cut(s) 579, 725, 820
AluBI AGCT 5 cut(s) 22, 91, 160, 573, 777
AluI AGCT 5 cut(s) 22, 91, 160, 573, 777
Alw21I GWGCWC 1 cut(s) 922
Alw26I GTCTC 4 cut(s) 203, 532, 591, 1153
AlwI GGATC 4 cut(s) 357, 370, 578, 591
AoxI GGCC 3 cut(s) 404, 818, 864
ApeKI GCWGC 1 cut(s) 88
ApoI RAATTY 1 cut(s) 910
Asp700I GAANNNNTTC 1 cut(s) 1107
AspLEI GCGC 2 cut(s) 297, 613
AspS9I GGNCC 2 cut(s) 864, 903
AsuC2I CCSGG 2 cut(s) 863, 1145
AsuHPI GGTGA 2 cut(s) 18, 319
AvaII GGWCC 1 cut(s) 903
BamHI GGATCC 2 cut(s) 362, 583
BarI GAAGNNNNNNTAC 2 cut(s) 1095, 1127
BbrPI CACGTG 1 cut(s) 854
Bbv12I GWGCWC 1 cut(s) 922
BbvI GCAGC 1 cut(s) 100
BccI CCATC 1 cut(s) 354
BcgI CGANNNNNNTGC 2 cut(s) 1146, 1180
BciT130I CCWGG 3 cut(s) 581, 727, 822
BcnI CCSGG 2 cut(s) 863, 1145
BcoDI GTCTC 4 cut(s) 203, 532, 591, 1153
BfaI CTAG 3 cut(s) 72, 320, 374
BfmI CTRYAG 1 cut(s) 135
BglII AGATCT 1 cut(s) 312
BisI GCNGC 2 cut(s) 89, 1155
BlsI GCNGC 2 cut(s) 90, 1156
Bme1390I CCNGG 5 cut(s) 581, 727, 822, 863, 1145
Bme18I GGWCC 1 cut(s) 903
BmeRI GACNNNNNGTC 1 cut(s) 1042
BmgT120I GGNCC 2 cut(s) 864, 903
BmiI GGNNCC 2 cut(s) 364, 585
BmrFI CCNGG 5 cut(s) 581, 727, 822, 863, 1145
BmsI GCATC 2 cut(s) 315, 1166
BplI GAGNNNNNCTC 2 cut(s) 588, 620
BpmI CTGGAG 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 1028
BpuMI CCSGG 2 cut(s) 863, 1145
BsaAI YACGTR 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 651
BsaI GGTCTC 3 cut(s) 532, 591, 1153
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse118I RCCGGY 2 cut(s) 28, 600
Bse1I ACTGG 2 cut(s) 159, 187
Bse3DI GCAATG 1 cut(s) 1047
BseBI CCWGG 3 cut(s) 581, 727, 822
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 3 cut(s) 294, 792, 1164
BseNI ACTGG 2 cut(s) 159, 187
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 100
BshFI GGCC 3 cut(s) 406, 820, 866
BsiHKAI GWGCWC 1 cut(s) 922
BsiSI CCGG 4 cut(s) 29, 601, 862, 1144
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 4 cut(s) 203, 532, 591, 1153
BsmI GAATGC 1 cut(s) 944
BsnI GGCC 3 cut(s) 406, 820, 866
Bso31I GGTCTC 3 cut(s) 532, 591, 1153
Bsp1286I GDGCHC 1 cut(s) 922
Bsp143I GATC 4 cut(s) 312, 362, 583, 893
BspACI CCGC 1 cut(s) 1155
BspANI GGCC 3 cut(s) 406, 820, 866
BspCNI CTCAG 3 cut(s) 295, 791, 1163
BspLI GGNNCC 2 cut(s) 364, 585
BspMAI CTGCAG 1 cut(s) 139
BspPI GGATC 4 cut(s) 357, 370, 578, 591
BspQI GCTCTTC 1 cut(s) 502
BspTNI GGTCTC 3 cut(s) 532, 591, 1153
BsrDI GCAATG 1 cut(s) 1047
BsrFI RCCGGY 2 cut(s) 28, 600
BsrI ACTGG 2 cut(s) 159, 187
BssAI RCCGGY 2 cut(s) 28, 600
BssMI GATC 4 cut(s) 312, 362, 583, 893
BssNAI GTATAC 1 cut(s) 152
BssNI GRCGYC 1 cut(s) 651
Bst1107I GTATAC 1 cut(s) 152
Bst2UI CCWGG 3 cut(s) 581, 727, 822
Bst4CI ACNGT 5 cut(s) 167, 276, 384, 924, 1057
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 1 cut(s) 651
BstBAI YACGTR 1 cut(s) 854
BstC8I GCNNGC 7 cut(s) 408, 792, 841, 845, 849, 1014, 1018
BstDEI CTNAG 4 cut(s) 303, 607, 778, 1150
BstHHI GCGC 2 cut(s) 297, 613
BstKTI GATC 4 cut(s) 315, 365, 586, 896
BstMAI GTCTC 4 cut(s) 203, 532, 591, 1153
BstMBI GATC 4 cut(s) 312, 362, 583, 893
BstMWI GCNNNNNNNGC 2 cut(s) 610, 863
BstNI CCWGG 3 cut(s) 581, 727, 822
BstNSI RCATGY 1 cut(s) 1016
BstSCI CCNGG 5 cut(s) 579, 725, 820, 861, 1143
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 100
BstX2I RGATCY 3 cut(s) 312, 362, 583
BstYI RGATCY 3 cut(s) 312, 362, 583
BstZ17I GTATAC 1 cut(s) 152
BsuRI GGCC 3 cut(s) 406, 820, 866
Cac8I GCNNGC 7 cut(s) 408, 792, 841, 845, 849, 1014, 1018
CfoI GCGC 2 cut(s) 297, 613
Cfr10I RCCGGY 2 cut(s) 28, 600
Cfr13I GGNCC 2 cut(s) 864, 903
CseI GACGC 1 cut(s) 640
Csp6I GTAC 1 cut(s) 545
CviAII CATG 3 cut(s) 395, 655, 1013
CviQI GTAC 1 cut(s) 545
DdeI CTNAG 4 cut(s) 303, 607, 778, 1150
DpnI GATC 4 cut(s) 314, 364, 585, 895
DpnII GATC 4 cut(s) 312, 362, 583, 893
DriI GACNNNNNGTC 1 cut(s) 1042
Eam1104I CTCTTC 1 cut(s) 502
Eam1105I GACNNNNNGTC 1 cut(s) 1042
EarI CTCTTC 1 cut(s) 502
Eco147I AGGCCT 1 cut(s) 820
Eco31I GGTCTC 3 cut(s) 532, 591, 1153
Eco47I GGWCC 1 cut(s) 903
Eco57I CTGAAG 3 cut(s) 32, 666, 741
Eco72I CACGTG 1 cut(s) 854
EcoRII CCWGG 3 cut(s) 579, 725, 820
FaeI CATG 3 cut(s) 398, 658, 1016
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FatI CATG 3 cut(s) 394, 654, 1012
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 2 cut(s) 89, 1155
Fsp4HI GCNGC 2 cut(s) 89, 1155
FspBI CTAG 3 cut(s) 72, 320, 374
FspI TGCGCA 1 cut(s) 296
GlaI GCGC 2 cut(s) 296, 612
GluI GCNGC 2 cut(s) 89, 1155
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 3 cut(s) 406, 820, 866
HapII CCGG 4 cut(s) 29, 601, 862, 1144
HgaI GACGC 1 cut(s) 640
HhaI GCGC 2 cut(s) 297, 613
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 3 cut(s) 398, 658, 1016
Hin6I GCGC 2 cut(s) 295, 611
HinP1I GCGC 2 cut(s) 295, 611
HincII GTYRAC 1 cut(s) 786
HindII GTYRAC 1 cut(s) 786
HinfI GANTC 6 cut(s) 7, 60, 211, 641, 689, 1037
HpaI GTTAAC 1 cut(s) 786
HpaII CCGG 4 cut(s) 29, 601, 862, 1144
HphI GGTGA 2 cut(s) 18, 319
Hpy166II GTNNAC 4 cut(s) 152, 427, 786, 1060
Hpy188III TCNNGA 9 cut(s) 72, 533, 596, 619, 871, 891, 906, 915, 1088
Hpy8I GTNNAC 4 cut(s) 152, 427, 786, 1060
HpyAV CCTTC 3 cut(s) 7, 641, 877
HpyCH4III ACNGT 5 cut(s) 167, 276, 384, 924, 1057
HpyCH4IV ACGT 3 cut(s) 148, 447, 853
HpyCH4V TGCA 5 cut(s) 137, 468, 839, 1020, 1052
HpyF10VI GCNNNNNNNGC 2 cut(s) 610, 863
HpyF3I CTNAG 4 cut(s) 303, 607, 778, 1150
HpySE526I ACGT 3 cut(s) 148, 447, 853
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 3 cut(s) 398, 658, 1016
HspAI GCGC 2 cut(s) 295, 611
KspAI GTTAAC 1 cut(s) 786
Kzo9I GATC 4 cut(s) 312, 362, 583, 893
LguI GCTCTTC 1 cut(s) 502
LmnI GCTCC 2 cut(s) 19, 157
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 2 cut(s) 315, 1166
MaeI CTAG 3 cut(s) 72, 320, 374
MaeII ACGT 3 cut(s) 148, 447, 853
MaeIII GTNAC 2 cut(s) 53, 223
MalI GATC 4 cut(s) 314, 364, 585, 895
MboI GATC 4 cut(s) 312, 362, 583, 893
MboII GAAGA 4 cut(s) 322, 489, 1068, 1180
MflI RGATCY 3 cut(s) 312, 362, 583
MhlI GDGCHC 1 cut(s) 922
MluCI AATT 2 cut(s) 910, 985
MlyI GAGTC 2 cut(s) 205, 1031
MmeI TCCRAC 2 cut(s) 288, 1104
MnlI CCTC 7 cut(s) 10, 214, 298, 376, 407, 497, 754
MroXI GAANNNNTTC 1 cut(s) 1107
MseI TTAA 4 cut(s) 39, 420, 785, 1029
MslI CAYNNNNRTG 1 cut(s) 937
MspI CCGG 4 cut(s) 29, 601, 862, 1144
MspR9I CCNGG 5 cut(s) 581, 727, 822, 863, 1145
Mva1269I GAATGC 1 cut(s) 944
MvaI CCWGG 3 cut(s) 581, 727, 822
MwoI GCNNNNNNNGC 2 cut(s) 610, 863
NciI CCSGG 2 cut(s) 863, 1145
NdeII GATC 4 cut(s) 312, 362, 583, 893
NlaIII CATG 3 cut(s) 398, 658, 1016
NlaIV GGNNCC 2 cut(s) 364, 585
NsbI TGCGCA 1 cut(s) 296
NspI RCATGY 1 cut(s) 1016
PaeI GCATGC 1 cut(s) 1016
PceI AGGCCT 1 cut(s) 820
PciSI GCTCTTC 1 cut(s) 502
PctI GAATGC 1 cut(s) 944
PdmI GAANNNNTTC 1 cut(s) 1107
PfeI GAWTC 4 cut(s) 7, 60, 641, 689
PfoI TCCNGGA 1 cut(s) 725
PkrI GCNGC 2 cut(s) 90, 1156
PleI GAGTC 2 cut(s) 205, 1031
PmaCI CACGTG 1 cut(s) 854
PmlI CACGTG 1 cut(s) 854
PpsI GAGTC 2 cut(s) 205, 1031
Ppu21I YACGTR 1 cut(s) 854
Psp6I CCWGG 3 cut(s) 579, 725, 820
PspCI CACGTG 1 cut(s) 854
PspGI CCWGG 3 cut(s) 579, 725, 820
PspN4I GGNNCC 2 cut(s) 364, 585
PspPI GGNCC 2 cut(s) 864, 903
PstI CTGCAG 1 cut(s) 139
PsuI RGATCY 3 cut(s) 312, 362, 583
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
RseI CAYNNNNRTG 1 cut(s) 937
SapI GCTCTTC 1 cut(s) 502
SaqAI TTAA 4 cut(s) 39, 420, 785, 1029
SatI GCNGC 2 cut(s) 89, 1155
Sau3AI GATC 4 cut(s) 312, 362, 583, 893
Sau96I GGNCC 2 cut(s) 864, 903
SchI GAGTC 2 cut(s) 205, 1031
ScrFI CCNGG 5 cut(s) 581, 727, 822, 863, 1145
SduI GDGCHC 1 cut(s) 922
SfaNI GCATC 2 cut(s) 315, 1166
SfcI CTRYAG 1 cut(s) 135
SinI GGWCC 1 cut(s) 903
SmiMI CAYNNNNRTG 1 cut(s) 937
SmlI CTYRAG 1 cut(s) 1007
SmoI CTYRAG 1 cut(s) 1007
SphI GCATGC 1 cut(s) 1016
Sse9I AATT 2 cut(s) 910, 985
SseBI AGGCCT 1 cut(s) 820
SsiI CCGC 1 cut(s) 1155
SspI AATATT 1 cut(s) 176
SspMI CTAG 3 cut(s) 72, 320, 374
StuI AGGCCT 1 cut(s) 820
StyD4I CCNGG 5 cut(s) 579, 725, 820, 861, 1143
TaaI ACNGT 5 cut(s) 167, 276, 384, 924, 1057
TaiI ACGT 3 cut(s) 151, 450, 856
TaqI TCGA 4 cut(s) 508, 595, 890, 1166
TasI AATT 2 cut(s) 910, 985
TauI GCSGC 1 cut(s) 1157
TfiI GAWTC 4 cut(s) 7, 60, 641, 689
Tru1I TTAA 4 cut(s) 39, 420, 785, 1029
Tru9I TTAA 4 cut(s) 39, 420, 785, 1029
TseI GCWGC 1 cut(s) 88
TspDTI ATGAA 3 cut(s) 368, 383, 786
VpaK11BI GGWCC 1 cut(s) 903
XapI RAATTY 1 cut(s) 910
XbaI TCTAGA 1 cut(s) 71
XceI RCATGY 1 cut(s) 1016
XmiI GTMKAC 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 1107
XspI CTAG 3 cut(s) 72, 320, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.