AT1G04230

rRNA-processing protein Efg1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008484
Physical Location & Seq
Forward (+)
1125515 .. 1128788
3274 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04230.1

Sequence Viewer

Length: 978 bp
ATGGCGCACGGCGGATATGCGAGGCGGAGAGTTGCAGAGCGGAAGACGACGGCAGGAACTAGTCGGAGATCGAAAGGATTGCGCGTGGAGAAAAAACCGAAGAATTCCTCTCTCAAGAATCAAATTCGTTCCATCGGTCGCATGATTCGTAAAGATCTTCCTCCTGAAGTAAGAGAGGCTCTAGAAAAGAAGTTGGATGATCTGAAGAAGCAGCAAGATATCCATTTCCGTCTTGCTTTTGAACGTAAAATCTTCCTAAGGAACAGAAAGGTCAGATTCTTCGAGCGGAGAAAGATTGAAAGGAGTATAAGACGTCTCGAGAAGCTACATCGGAGTACATCTGGTGGTTATGTGCAAGATGCAGAAATAGGTGGGCAACTCAATAGACTCAAAGAAGACCTTGAATATGTTAGGTTCTTCCCCAAGAATGAGAAGTATGTATCGCTATTTAGTGGATCTGATGATTTACAAGTATCTGAGCGGAGAAGTAAATTGCGGAAGCAAATCAAAGCCAACATAATTTTTGCTGCTGCCAGTGGGAAAGAGTTGGAAGAGACAGGGAGCGAAGATGATGCTCTTTTGGACCTGAGTGACGATGACTTCTTTGTCAACGGAAGTTCAAGTGACGAAGCAGATGCGGATGATGAGTGGACTGATAAAAGCACAAAAGAGCCGGTTTCTAGTGCTTCTGGTAGAGCAACATCGAGCATGTCCAGTGACGAAAGAAATCAGAAGCCATACTCTACTAGGGTTTTAATGCCACCACCACGGTCAAGGTTTGCATCAACATCTCGTCAGTATTCATCTGTGAAGAGGAATGAGATACCTTCCTCCAGCAACACTTCACATAGAAGAAGCCAGTCTTCACATGCTGCCACTAGTTCGCATACAAGTCAGAGTAGCAACTTGAGTTCTAATTCTGATGCTCACAAACCCAAGAGGAAGAGAAGGCCTAAGAAGAAGAAGCTACAGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Efg1

Protein Analysis

325

Amino Acids

37.15

Weight (kDa)

10.31

Isoelectric Point (pI)

67.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Efg1 PF10153 38 - 150 3.2e-28 rRNA-processing protein Efg1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 605
AatII GACGTC 1 cut(s) 316
AccBSI CCGCTC 3 cut(s) 40, 286, 481
AccII CGCG 1 cut(s) 84
AciI CCGC 7 cut(s) 12, 25, 40, 286, 481, 496, 638
AclWI GGATC 1 cut(s) 463
AcsI RAATTY 2 cut(s) 103, 123
AcuI CTGAAG 2 cut(s) 186, 224
AcyI GRCGYC 1 cut(s) 313
AfaI GTAC 1 cut(s) 337
AgsI TTSAA 4 cut(s) 242, 299, 404, 621
AhlI ACTAGT 2 cut(s) 59, 878
AluBI AGCT 2 cut(s) 325, 967
AluI AGCT 2 cut(s) 325, 967
Alw26I GTCTC 2 cut(s) 320, 548
AlwI GGATC 1 cut(s) 463
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 1 cut(s) 950
ApeKI GCWGC 4 cut(s) 211, 527, 530, 872
ApoI RAATTY 2 cut(s) 103, 123
AspLEI GCGC 2 cut(s) 7, 84
AspS9I GGNCC 1 cut(s) 583
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 583
AxyI CCTNAGG 1 cut(s) 257
BbsI GAAGAC 3 cut(s) 50, 402, 855
BbvI GCAGC 4 cut(s) 223, 514, 517, 859
BccI CCATC 1 cut(s) 140
BceAI ACGGC 2 cut(s) 25, 66
BcgI CGANNNNNNTGC 6 cut(s) 61, 95, 554, 588, 617, 651
BcoDI GTCTC 2 cut(s) 320, 548
BcuI ACTAGT 2 cut(s) 59, 878
BfaI CTAG 5 cut(s) 60, 182, 681, 747, 879
BfmI CTRYAG 1 cut(s) 968
BglII AGATCT 1 cut(s) 154
BisI GCNGC 4 cut(s) 212, 528, 531, 873
BlsI GCNGC 4 cut(s) 213, 529, 532, 874
Bme18I GGWCC 1 cut(s) 583
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 1 cut(s) 583
BmsI GCATC 5 cut(s) 349, 562, 625, 791, 913
BpiI GAAGAC 3 cut(s) 50, 402, 855
BpmI CTGGAG 1 cut(s) 817
BpuEI CTTGAG 2 cut(s) 98, 928
BsaHI GRCGYC 1 cut(s) 313
BsaJI CCNNGG 1 cut(s) 767
Bse118I RCCGGY 1 cut(s) 673
Bse1I ACTGG 3 cut(s) 534, 714, 859
Bse21I CCTNAGG 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 767
BseGI GGATG 2 cut(s) 202, 646
BseMII CTCAG 2 cut(s) 468, 578
BseNI ACTGG 3 cut(s) 534, 714, 859
BseXI GCAGC 4 cut(s) 223, 514, 517, 859
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 1 cut(s) 952
BsiEI CGRYCG 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 1 cut(s) 674
BsmAI GTCTC 2 cut(s) 320, 548
BsmBI CGTCTC 1 cut(s) 320
BsnI GGCC 1 cut(s) 952
BsoBI CYCGRG 1 cut(s) 317
Bsp143I GATC 4 cut(s) 68, 154, 199, 455
BspACI CCGC 7 cut(s) 12, 25, 40, 286, 481, 496, 638
BspANI GGCC 1 cut(s) 952
BspCNI CTCAG 2 cut(s) 469, 579
BspFNI CGCG 1 cut(s) 84
BspPI GGATC 1 cut(s) 463
BsrBI CCGCTC 3 cut(s) 40, 286, 481
BsrFI RCCGGY 1 cut(s) 673
BsrI ACTGG 3 cut(s) 534, 714, 859
BssAI RCCGGY 1 cut(s) 673
BssECI CCNNGG 1 cut(s) 767
BssMI GATC 4 cut(s) 68, 154, 199, 455
BssNI GRCGYC 1 cut(s) 313
Bst4CI ACNGT 1 cut(s) 771
Bst6I CTCTTC 3 cut(s) 546, 806, 938
BstACI GRCGYC 1 cut(s) 313
BstDEI CTNAG 4 cut(s) 257, 477, 587, 954
BstDSI CCRYGG 1 cut(s) 767
BstF5I GGATG 2 cut(s) 202, 646
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 2 cut(s) 7, 84
BstKTI GATC 4 cut(s) 71, 157, 202, 458
BstMAI GTCTC 2 cut(s) 320, 548
BstMBI GATC 4 cut(s) 68, 154, 199, 455
BstMCI CGRYCG 1 cut(s) 139
BstNSI RCATGY 2 cut(s) 712, 872
BstSFI CTRYAG 1 cut(s) 968
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 4 cut(s) 223, 514, 517, 859
BstV2I GAAGAC 3 cut(s) 50, 402, 855
BstX2I RGATCY 2 cut(s) 154, 455
BstYI RGATCY 2 cut(s) 154, 455
Bsu36I CCTNAGG 1 cut(s) 257
BsuRI GGCC 1 cut(s) 952
BtgI CCRYGG 1 cut(s) 767
BtsCI GGATG 2 cut(s) 202, 646
BtsIMutI CAGTG 2 cut(s) 541, 721
CfoI GCGC 2 cut(s) 7, 84
Cfr10I RCCGGY 1 cut(s) 673
Cfr13I GGNCC 1 cut(s) 583
Csp6I GTAC 1 cut(s) 336
CviAII CATG 3 cut(s) 142, 709, 869
CviJI RGCY 8 cut(s) 179, 325, 512, 673, 736, 858, 952, 967
CviKI_1 RGCY 8 cut(s) 179, 325, 512, 673, 736, 858, 952, 967
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 4 cut(s) 257, 477, 587, 954
DpnI GATC 4 cut(s) 70, 156, 201, 457
DpnII GATC 4 cut(s) 68, 154, 199, 455
DrdI GACNNNNNNGTC 1 cut(s) 605
DseDI GACNNNNNNGTC 1 cut(s) 605
Eam1104I CTCTTC 3 cut(s) 546, 806, 938
EarI CTCTTC 3 cut(s) 546, 806, 938
EciI GGCGGA 2 cut(s) 27, 40
Eco147I AGGCCT 1 cut(s) 952
Eco32I GATATC 1 cut(s) 220
Eco47I GGWCC 1 cut(s) 583
Eco57I CTGAAG 2 cut(s) 186, 224
Eco81I CCTNAGG 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 317
EcoRI GAATTC 1 cut(s) 103
EcoRV GATATC 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 3 cut(s) 145, 712, 872
FalI AAGNNNNNCTT 4 cut(s) 384, 416, 847, 879
FatI CATG 3 cut(s) 141, 708, 868
Fnu4HI GCNGC 4 cut(s) 212, 528, 531, 873
FokI GGATG 2 cut(s) 209, 653
Fsp4HI GCNGC 4 cut(s) 212, 528, 531, 873
FspBI CTAG 5 cut(s) 60, 182, 681, 747, 879
GlaI GCGC 2 cut(s) 6, 83
GluI GCNGC 4 cut(s) 212, 528, 531, 873
GsuI CTGGAG 1 cut(s) 817
HaeIII GGCC 1 cut(s) 952
HapII CCGG 1 cut(s) 674
HhaI GCGC 2 cut(s) 7, 84
Hin1I GRCGYC 1 cut(s) 313
Hin1II CATG 3 cut(s) 145, 712, 872
Hin6I GCGC 2 cut(s) 5, 82
HinP1I GCGC 2 cut(s) 5, 82
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 4 cut(s) 118, 145, 276, 387
HpaII CCGG 1 cut(s) 674
Hpy166II GTNNAC 2 cut(s) 610, 651
Hpy188I TCNGA 9 cut(s) 66, 204, 275, 333, 460, 478, 732, 897, 922
Hpy188III TCNNGA 5 cut(s) 115, 164, 182, 317, 319
Hpy8I GTNNAC 2 cut(s) 610, 651
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 2 cut(s) 837, 942
HpyCH4III ACNGT 1 cut(s) 771
HpyCH4IV ACGT 2 cut(s) 244, 313
HpyCH4V TGCA 4 cut(s) 35, 355, 362, 782
HpyF3I CTNAG 4 cut(s) 257, 477, 587, 954
HpySE526I ACGT 2 cut(s) 244, 313
Hsp92I GRCGYC 1 cut(s) 313
Hsp92II CATG 3 cut(s) 145, 712, 872
HspAI GCGC 2 cut(s) 5, 82
Kzo9I GATC 4 cut(s) 68, 154, 199, 455
LmnI GCTCC 1 cut(s) 561
Lsp1109I GCAGC 4 cut(s) 223, 514, 517, 859
LweI GCATC 5 cut(s) 349, 562, 625, 791, 913
MaeI CTAG 5 cut(s) 60, 182, 681, 747, 879
MaeII ACGT 2 cut(s) 244, 313
MaeIII GTNAC 3 cut(s) 590, 623, 716
MalI GATC 4 cut(s) 70, 156, 201, 457
MbiI CCGCTC 3 cut(s) 40, 286, 481
MboI GATC 4 cut(s) 68, 154, 199, 455
MflI RGATCY 2 cut(s) 154, 455
MluCI AATT 5 cut(s) 103, 123, 491, 519, 916
MlyI GAGTC 1 cut(s) 381
MmeI TCCRAC 3 cut(s) 44, 174, 528
MnlI CCTC 7 cut(s) 15, 118, 169, 171, 807, 841, 933
MseI TTAA 1 cut(s) 755
MspI CCGG 1 cut(s) 674
MvnI CGCG 1 cut(s) 84
NdeII GATC 4 cut(s) 68, 154, 199, 455
NlaIII CATG 3 cut(s) 145, 712, 872
NmuCI GTSAC 3 cut(s) 590, 623, 716
NspI RCATGY 2 cut(s) 712, 872
PaeR7I CTCGAG 1 cut(s) 317
PceI AGGCCT 1 cut(s) 952
PcsI WCGNNNNNNNCGW 1 cut(s) 145
PfeI GAWTC 3 cut(s) 118, 145, 276
PkrI GCNGC 4 cut(s) 213, 529, 532, 874
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
PspPI GGNCC 1 cut(s) 583
PsuI RGATCY 2 cut(s) 154, 455
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 755
SatI GCNGC 4 cut(s) 212, 528, 531, 873
Sau3AI GATC 4 cut(s) 68, 154, 199, 455
Sau96I GGNCC 1 cut(s) 583
SchI GAGTC 1 cut(s) 381
SfaNI GCATC 5 cut(s) 349, 562, 625, 791, 913
SfcI CTRYAG 1 cut(s) 968
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 1 cut(s) 583
SlaI CTCGAG 1 cut(s) 317
SmlI CTYRAG 3 cut(s) 113, 317, 907
SmoI CTYRAG 3 cut(s) 113, 317, 907
SpeI ACTAGT 2 cut(s) 59, 878
Sse9I AATT 5 cut(s) 103, 123, 491, 519, 916
SseBI AGGCCT 1 cut(s) 952
SsiI CCGC 7 cut(s) 12, 25, 40, 286, 481, 496, 638
SspMI CTAG 5 cut(s) 60, 182, 681, 747, 879
StuI AGGCCT 1 cut(s) 952
TaaI ACNGT 1 cut(s) 771
TaiI ACGT 2 cut(s) 247, 316
TaqI TCGA 4 cut(s) 71, 282, 318, 704
TaqII GACCGA 1 cut(s) 125
TasI AATT 5 cut(s) 103, 123, 491, 519, 916
TatI WGTACW 1 cut(s) 335
TfiI GAWTC 3 cut(s) 118, 145, 276
Tru1I TTAA 1 cut(s) 755
Tru9I TTAA 1 cut(s) 755
TscAI CASTG 2 cut(s) 541, 721
TseFI GTSAC 3 cut(s) 590, 623, 716
TseI GCWGC 4 cut(s) 211, 527, 530, 872
Tsp45I GTSAC 3 cut(s) 590, 623, 716
TspDTI ATGAA 1 cut(s) 792
TspGWI ACGGA 2 cut(s) 218, 627
TspRI CASTG 2 cut(s) 541, 721
VpaK11BI GGWCC 1 cut(s) 583
XapI RAATTY 2 cut(s) 103, 123
XbaI TCTAGA 1 cut(s) 181
XceI RCATGY 2 cut(s) 712, 872
XhoI CTCGAG 1 cut(s) 317
XspI CTAG 5 cut(s) 60, 182, 681, 747, 879
ZraI GACGTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.