pycom16g17340

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008484
Physical Location & Seq
Forward (+)
13445346 .. 13448119
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g17340.1

Sequence Viewer

Length: 963 bp
ATGGCTCACGGTGGCTACAGCAAGCGGCGGGTGAACCCCGCCGGACGTCGGTCCAAGTCGGCCTTGGGCCCGGAGAAGAAGCCCAAGTCCGTCACCCTCAAAAACCAGATTCGCTCCATCGAGCGCATGCTCCGTAAGAATCTACCAGCTGAAGTTAGGGAGGCTCAGGAAAAGAAGTTGGAAGGATTCAAGAAACAACAGGAGATTCATTCCCGTCTCGCTGTGGAACGCAAAATATTTATGCGTGACAGGAAGATAAAGTTTTTTGAAAGAAGAAAGATAGAGAGAAGAGTAAGACGCCTGGAGAAACTCCAACGTGCTTCATCGGGTCAGGCACAAGACGCAGAGGTTTCTGTGAACCTTTCGAAATTGAAAGAAGATCTTGAATATGTTAGGTTCTTTCCCAAGACCGAAAAGTATGTCTCTCTATTTACTGGAGGTGAAGATACAGATGTGATTGATAAGAGAAATAAGTTGCGAAAGCAGATTAAGGCAAATATAGTTGCTGCAGCAGCTAGCGGAAAGGATTTGGAAGAAACAGGGAGTGAGGATGATGGGCTTCTGGATATGAGCGAAGATGATTTCTTCCTAAATGGGAGTTCTAGTGATGAAGCAGATGCAGATGATGAATGGACAGATAAGAGTACAAAGGAACCGGTGTCCAGTGCTTCAGGAAAAGCAACTTCTGGCATGTCCAGTGATGAGAGAAATCAGCGACAGATTTCTGCTAGAGCTTTGATGCCTCCTCCCCGGCCGTCCAGCAATTCTCGCCCAAGTTCTGTTCGTGCTCAGTCAAGGTTTGGTCCTTCATCAAGCAAAAATTCCTCAAAAAGCATTGCTGAAATGGCCACATCAAGCGATACATCAAATAGCAGAAGTGGAACTTCCTTCAATACATCAAATAGCAATACATCAAGTGGCAGAAGTGGAACTTCGTTCAAAGCTAGGGGATCGCTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Efg1

Protein Analysis

321

Amino Acids

35.61

Weight (kDa)

10.08

Isoelectric Point (pI)

57.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Efg1 PF10153 33 - 144 6.6e-31 rRNA-processing protein Efg1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 49
AciI CCGC 4 cut(s) 25, 28, 39, 519
AclWI GGATC 1 cut(s) 958
AcoI YGGCCR 2 cut(s) 752, 846
AcsI RAATTY 1 cut(s) 820
AcuI CTGAAG 2 cut(s) 171, 654
AcyI GRCGYC 2 cut(s) 46, 298
AfaI GTAC 1 cut(s) 646
AfiI CCNNNNNNNGG 1 cut(s) 48
AgeI ACCGGT 1 cut(s) 655
AgsI TTSAA 6 cut(s) 190, 269, 373, 386, 892, 940
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 4 cut(s) 149, 515, 734, 944
AluI AGCT 4 cut(s) 149, 515, 734, 944
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 2 cut(s) 221, 427
AlwI GGATC 1 cut(s) 958
AoxI GGCC 4 cut(s) 60, 67, 752, 846
ApaI GGGCCC 1 cut(s) 71
ApeKI GCWGC 3 cut(s) 506, 509, 512
ApoI RAATTY 1 cut(s) 820
ArsI GACNNNNNNTTYG 2 cut(s) 405, 437
AsiGI ACCGGT 1 cut(s) 655
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 4 cut(s) 51, 67, 68, 803
AsuC2I CCSGG 2 cut(s) 71, 751
AsuHPI GGTGA 3 cut(s) 43, 85, 452
AsuII TTCGAA 1 cut(s) 365
AsuNHI GCTAGC 1 cut(s) 515
AvaII GGWCC 2 cut(s) 51, 803
BaeGI GKGCMC 1 cut(s) 71
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BalI TGGCCA 1 cut(s) 848
BanII GRGCYC 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 3 cut(s) 493, 521, 524
BccI CCATC 2 cut(s) 125, 548
BceAI ACGGC 1 cut(s) 739
BciT130I CCWGG 1 cut(s) 302
BcnI CCSGG 2 cut(s) 71, 751
BcoDI GTCTC 2 cut(s) 221, 427
BfaI CTAG 4 cut(s) 516, 603, 729, 945
BfmI CTRYAG 2 cut(s) 16, 507
BglII AGATCT 1 cut(s) 379
BisI GCNGC 4 cut(s) 26, 507, 510, 513
BlsI GCNGC 4 cut(s) 27, 508, 511, 514
Bme1390I CCNGG 3 cut(s) 71, 302, 751
Bme18I GGWCC 2 cut(s) 51, 803
BmgT120I GGNCC 4 cut(s) 51, 67, 68, 803
BmiI GGNNCC 2 cut(s) 69, 654
BmrFI CCNGG 3 cut(s) 71, 302, 751
BmsI GCATC 2 cut(s) 607, 729
BmtI GCTAGC 1 cut(s) 519
BoxI GACNNNNGTC 1 cut(s) 49
BpmI CTGGAG 2 cut(s) 323, 456
Bpu10I CCTNAGC 2 cut(s) 165, 956
Bpu14I TTCGAA 1 cut(s) 365
BpuMI CCSGG 2 cut(s) 71, 751
BsaHI GRCGYC 2 cut(s) 46, 298
BsaJI CCNNGG 2 cut(s) 63, 749
BsaWI WCCGGW 1 cut(s) 655
BsaXI ACNNNNNCTCC 2 cut(s) 589, 619
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse118I RCCGGY 1 cut(s) 655
Bse1I ACTGG 3 cut(s) 439, 663, 696
Bse3DI GCAATG 1 cut(s) 834
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 2 cut(s) 63, 749
BseGI GGATG 1 cut(s) 556
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 2 cut(s) 179, 803
BseNI ACTGG 3 cut(s) 439, 663, 696
BseRI GAGGAG 1 cut(s) 735
BseSI GKGCMC 1 cut(s) 71
BseX3I CGGCCG 1 cut(s) 752
BseXI GCAGC 3 cut(s) 493, 521, 524
Bsh1285I CGRYCG 1 cut(s) 755
BshFI GGCC 4 cut(s) 62, 69, 754, 848
BshTI ACCGGT 1 cut(s) 655
BsiEI CGRYCG 1 cut(s) 755
BsiHKAI GWGCWC 1 cut(s) 790
BsiSI CCGG 4 cut(s) 42, 71, 656, 751
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 2 cut(s) 221, 427
BsmBI CGTCTC 1 cut(s) 221
BsnI GGCC 4 cut(s) 62, 69, 754, 848
Bsp119I TTCGAA 1 cut(s) 365
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 2 cut(s) 71, 790
Bsp143I GATC 2 cut(s) 379, 950
BspACI CCGC 4 cut(s) 25, 28, 39, 519
BspANI GGCC 4 cut(s) 62, 69, 754, 848
BspCNI CTCAG 2 cut(s) 178, 802
BspLI GGNNCC 2 cut(s) 69, 654
BspMAI CTGCAG 1 cut(s) 511
BspOI GCTAGC 1 cut(s) 519
BspPI GGATC 1 cut(s) 958
BspT104I TTCGAA 1 cut(s) 365
BsrDI GCAATG 1 cut(s) 834
BsrFI RCCGGY 1 cut(s) 655
BsrI ACTGG 3 cut(s) 439, 663, 696
BssAI RCCGGY 1 cut(s) 655
BssECI CCNNGG 2 cut(s) 63, 749
BssMI GATC 2 cut(s) 379, 950
BssNI GRCGYC 2 cut(s) 46, 298
BssT1I CCWWGG 1 cut(s) 63
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 1 cut(s) 283
BstACI GRCGYC 2 cut(s) 46, 298
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 3 cut(s) 23, 128, 517
BstDEI CTNAG 3 cut(s) 165, 789, 956
BstF5I GGATG 1 cut(s) 556
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 2 cut(s) 382, 953
BstMAI GTCTC 2 cut(s) 221, 427
BstMBI GATC 2 cut(s) 379, 950
BstMCI CGRYCG 1 cut(s) 755
BstMWI GCNNNNNNNGC 4 cut(s) 341, 512, 768, 845
BstNI CCWGG 1 cut(s) 302
BstNSI RCATGY 2 cut(s) 130, 694
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 3 cut(s) 69, 300, 749
BstSFI CTRYAG 2 cut(s) 16, 507
BstSLI GKGCMC 1 cut(s) 71
BstV1I GCAGC 3 cut(s) 493, 521, 524
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BstZI CGGCCG 1 cut(s) 752
BsuRI GGCC 4 cut(s) 62, 69, 754, 848
BtsCI GGATG 1 cut(s) 556
BtsIMutI CAGTG 2 cut(s) 670, 703
Cac8I GCNNGC 3 cut(s) 23, 128, 517
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 655
Cfr13I GGNCC 4 cut(s) 51, 67, 68, 803
CseI GACGC 2 cut(s) 306, 350
Csp6I GTAC 1 cut(s) 645
CspAI ACCGGT 1 cut(s) 655
CviAII CATG 2 cut(s) 127, 691
CviQI GTAC 1 cut(s) 645
DdeI CTNAG 3 cut(s) 165, 789, 956
DpnI GATC 2 cut(s) 381, 952
DpnII GATC 2 cut(s) 379, 950
EaeI YGGCCR 2 cut(s) 752, 846
EagI CGGCCG 1 cut(s) 752
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
EclXI CGGCCG 1 cut(s) 752
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 71
Eco47I GGWCC 2 cut(s) 51, 803
Eco52I CGGCCG 1 cut(s) 752
Eco57I CTGAAG 2 cut(s) 171, 654
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 2 cut(s) 130, 694
FaiI YATR 7 cut(s) 128, 242, 390, 420, 500, 569, 692
FalI AAGNNNNNCTT 8 cut(s) 47, 79, 366, 398, 868, 900, 916, 948
FatI CATG 2 cut(s) 126, 690
FauI CCCGC 2 cut(s) 21, 46
Fnu4HI GCNGC 4 cut(s) 26, 507, 510, 513
FokI GGATG 1 cut(s) 563
FriOI GRGCYC 1 cut(s) 71
Fsp4HI GCNGC 4 cut(s) 26, 507, 510, 513
FspBI CTAG 4 cut(s) 516, 603, 729, 945
GlaI GCGC 1 cut(s) 125
GluI GCNGC 4 cut(s) 26, 507, 510, 513
GsuI CTGGAG 2 cut(s) 323, 456
HaeIII GGCC 4 cut(s) 62, 69, 754, 848
HapII CCGG 4 cut(s) 42, 71, 656, 751
HgaI GACGC 2 cut(s) 306, 350
HhaI GCGC 1 cut(s) 126
Hin1I GRCGYC 2 cut(s) 46, 298
Hin1II CATG 2 cut(s) 130, 694
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HinfI GANTC 4 cut(s) 109, 139, 186, 205
HpaII CCGG 4 cut(s) 42, 71, 656, 751
HphI GGTGA 3 cut(s) 43, 85, 452
Hpy166II GTNNAC 2 cut(s) 34, 358
Hpy188III TCNNGA 5 cut(s) 167, 190, 383, 563, 672
Hpy8I GTNNAC 2 cut(s) 34, 358
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 3 cut(s) 176, 816, 898
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 2 cut(s) 46, 316
HpyCH4V TGCA 2 cut(s) 509, 620
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 512, 768, 845
HpyF3I CTNAG 3 cut(s) 165, 789, 956
HpySE526I ACGT 2 cut(s) 46, 316
Hsp92I GRCGYC 2 cut(s) 46, 298
Hsp92II CATG 2 cut(s) 130, 694
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 379, 950
LmnI GCTCC 2 cut(s) 119, 135
Lsp1109I GCAGC 3 cut(s) 493, 521, 524
LweI GCATC 2 cut(s) 607, 729
MaeI CTAG 4 cut(s) 516, 603, 729, 945
MaeII ACGT 2 cut(s) 46, 316
MaeIII GTNAC 2 cut(s) 91, 245
MalI GATC 2 cut(s) 381, 952
MboI GATC 2 cut(s) 379, 950
MboII GAAGA 9 cut(s) 88, 265, 285, 300, 389, 455, 545, 577, 587
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 2 cut(s) 71, 790
MlsI TGGCCA 1 cut(s) 848
MluCI AATT 3 cut(s) 368, 763, 820
MluNI TGGCCA 1 cut(s) 848
MmeI TCCRAC 2 cut(s) 159, 337
MnlI CCTC 8 cut(s) 107, 154, 340, 431, 541, 753, 756, 835
Mox20I TGGCCA 1 cut(s) 848
MscI TGGCCA 1 cut(s) 848
MseI TTAA 1 cut(s) 489
Msp20I TGGCCA 1 cut(s) 848
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 4 cut(s) 42, 71, 656, 751
MspR9I CCNGG 3 cut(s) 71, 302, 751
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 4 cut(s) 341, 512, 768, 845
NciI CCSGG 2 cut(s) 71, 751
NdeII GATC 2 cut(s) 379, 950
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 2 cut(s) 130, 694
NlaIV GGNNCC 2 cut(s) 69, 654
NmuCI GTSAC 2 cut(s) 91, 245
NspI RCATGY 2 cut(s) 130, 694
NspV TTCGAA 1 cut(s) 365
PaeI GCATGC 1 cut(s) 130
PfeI GAWTC 4 cut(s) 109, 139, 186, 205
PinAI ACCGGT 1 cut(s) 655
PkrI GCNGC 4 cut(s) 27, 508, 511, 514
PshAI GACNNNNGTC 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 2 cut(s) 69, 654
PspOMI GGGCCC 1 cut(s) 67
PspPI GGNCC 4 cut(s) 51, 67, 68, 803
PstI CTGCAG 1 cut(s) 511
PsuI RGATCY 1 cut(s) 379
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 1 cut(s) 646
RsaNI GTAC 1 cut(s) 645
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 4 cut(s) 26, 507, 510, 513
Sau3AI GATC 2 cut(s) 379, 950
Sau96I GGNCC 4 cut(s) 51, 67, 68, 803
ScrFI CCNGG 3 cut(s) 71, 302, 751
SduI GDGCHC 2 cut(s) 71, 790
SfaNI GCATC 2 cut(s) 607, 729
SfcI CTRYAG 2 cut(s) 16, 507
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 2 cut(s) 51, 803
SphI GCATGC 1 cut(s) 130
Sse9I AATT 3 cut(s) 368, 763, 820
SsiI CCGC 4 cut(s) 25, 28, 39, 519
SspI AATATT 1 cut(s) 237
SspMI CTAG 4 cut(s) 516, 603, 729, 945
StyD4I CCNGG 3 cut(s) 69, 300, 749
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 2 cut(s) 49, 319
TaqI TCGA 2 cut(s) 120, 365
TaqII GACCGA 2 cut(s) 39, 425
TasI AATT 3 cut(s) 368, 763, 820
TatI WGTACW 1 cut(s) 644
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 4 cut(s) 109, 139, 186, 205
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 2 cut(s) 670, 703
TseFI GTSAC 2 cut(s) 91, 245
TseI GCWGC 3 cut(s) 506, 509, 512
Tsp45I GTSAC 2 cut(s) 91, 245
TspDTI ATGAA 5 cut(s) 197, 312, 624, 642, 798
TspGWI ACGGA 2 cut(s) 79, 122
TspRI CASTG 2 cut(s) 670, 703
VpaK11BI GGWCC 2 cut(s) 51, 803
XapI RAATTY 1 cut(s) 820
XceI RCATGY 2 cut(s) 130, 694
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XspI CTAG 4 cut(s) 516, 603, 729, 945
ZraI GACGTC 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.