Rw5G027230

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008484
Physical Location & Seq
Reverse (-)
39954347 .. 39958918
4572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027230.1

Sequence Viewer

Length: 1047 bp
ATGGCTCACGGCGGCTACGGAAAGCGGAGGGTGAAGCCTGCTGCCGGCCGTCGCTCCCGGACGGGCTTGGGCGTCGAGAAGAAGCCCAAGTCCGCCTCCCTCAAGAATCAGATTCGCTCCGTCGAGCGCATGCTCCGTAAGAATCTACCGCCTGAAGTTAGGGAGGTTCAGGAAAAGAAGTTAGAAGGACTCAAGAAACAGCAAGATATTCATACTCGGCTTGCAGTAGAACGCAAAATATTTTTGCGAGACCGGAAGATAAAATTTTTTGAGAGAAGAAAGATAGAGAGAAGAATAAGACGCTTGGAGAAACTTCAACGTACTACAGCTGCTCAGGTTCAAGATGCAGAGAATTCTGTGCAACTTTCCAACTTGAAAGAAGATCTTGAATATGTTAGGTTCTTTCCCAAGACCGAGAAATATGTCGCTCTGTTTACTGGAGGTGATGATTCAGAGCTTGTTGATAGGAGAAATAGGCTGCGTAAGCAGATTAAGGCCAATTTAATTGCTGCAGCTGCTAGTGGGAAGGATTTGGAAGAAACAGGGAGTGAGGATGATGGGCTTTTGGATCTCAGTGAAGATGATTTCTTTCTAAGCGGAAGCTCAAGTGATGAAGCAAATGCAGATGATGAATGGACAGATAAGAGTACAAGGGAACAGGCTTCCAGTGCTTCTGGGAAAGCAGCATCTGGCATGTCAAGTGATGAGAGAAATCAGAGACAGATTTCAGCTAGAGCTTTGATGCCTCCCCCTAGGCCATCAAGCAATTCTCGCTCAAGTTCAGTACGTGCTCAATCAAGATTCGGTCCTTCATCAAGCAAGAGTTCATCAAAACGTGCTGAAATGTCTACATCCAGCAATACATCTAGTAGCAGAAGTGGAACTTCTTTTAAAGCTAGGGGTTCCTCAAGTTCCGGGGCAACTCAAAGTAGTAATCTGAGCTCCAACTCTGATGCTCATAAACCCCGTAGTAATCTGAGCTCCAACTCTGATGCTCATAAACCCCGTAGAAAGAGGAGGCCAAAAAAGAAAAAGCAGCAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Efg1

Protein Analysis

348

Amino Acids

38.81

Weight (kDa)

10.53

Isoelectric Point (pI)

64.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Efg1 PF10153 34 - 145 1.9e-29 rRNA-processing protein Efg1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 848
AciI CCGC 5 cut(s) 12, 25, 93, 149, 597
AclWI GGATC 1 cut(s) 576
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 263, 352
AcuI CTGAAG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 322, 649, 786
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 4 cut(s) 317, 341, 376, 389
AluBI AGCT 9 cut(s) 329, 457, 515, 603, 731, 737, 896, 942, 981
AluI AGCT 9 cut(s) 329, 457, 515, 603, 731, 737, 896, 942, 981
Alw21I GWGCWC 3 cut(s) 793, 944, 983
Alw26I GTCTC 2 cut(s) 243, 712
AlwI GGATC 1 cut(s) 576
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 4 cut(s) 46, 495, 755, 1019
ApeKI GCWGC 8 cut(s) 41, 329, 478, 509, 512, 515, 683, 1036
ApoI RAATTY 2 cut(s) 263, 352
AspA2I CCTAGG 1 cut(s) 752
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 806
AsuC2I CCSGG 2 cut(s) 58, 916
AsuHPI GGTGA 2 cut(s) 43, 455
AvaII GGWCC 1 cut(s) 806
AvrII CCTAGG 1 cut(s) 752
BanII GRGCYC 2 cut(s) 944, 983
Bbv12I GWGCWC 3 cut(s) 793, 944, 983
BbvI GCAGC 7 cut(s) 28, 316, 465, 496, 502, 524, 695
BccI CCATC 2 cut(s) 551, 766
BceAI ACGGC 2 cut(s) 25, 33
BcnI CCSGG 2 cut(s) 58, 916
BcoDI GTCTC 2 cut(s) 243, 712
BfaI CTAG 5 cut(s) 519, 732, 753, 867, 897
BfmI CTRYAG 2 cut(s) 324, 510
BglII AGATCT 1 cut(s) 382
BisI GCNGC 9 cut(s) 13, 42, 330, 479, 510, 513, 516, 684, 1037
BlnI CCTAGG 1 cut(s) 752
BlsI GCNGC 9 cut(s) 14, 43, 331, 480, 511, 514, 517, 685, 1038
Bme1390I CCNGG 2 cut(s) 58, 916
Bme18I GGWCC 1 cut(s) 806
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 1 cut(s) 904
BmrFI CCNGG 2 cut(s) 58, 916
BmsI GCATC 5 cut(s) 334, 695, 732, 943, 982
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 5 cut(s) 86, 176, 589, 760, 892
BpuMI CCSGG 2 cut(s) 58, 916
BsaAI YACGTR 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 2 cut(s) 752, 915
BsaWI WCCGGW 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 2 cut(s) 442, 666
BseDI CCNNGG 2 cut(s) 752, 915
BseGI GGATG 2 cut(s) 559, 851
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 4 cut(s) 347, 586, 929, 968
BseNI ACTGG 2 cut(s) 442, 666
BseRI GAGGAG 1 cut(s) 1030
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 7 cut(s) 28, 316, 465, 496, 502, 524, 695
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 4 cut(s) 48, 497, 757, 1021
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 3 cut(s) 793, 944, 983
BsiSI CCGG 4 cut(s) 45, 58, 253, 915
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 2 cut(s) 243, 712
BsnI GGCC 4 cut(s) 48, 497, 757, 1021
Bso31I GGTCTC 1 cut(s) 243
Bsp1286I GDGCHC 3 cut(s) 793, 944, 983
Bsp143I GATC 2 cut(s) 382, 568
BspACI CCGC 5 cut(s) 12, 25, 93, 149, 597
BspANI GGCC 4 cut(s) 48, 497, 757, 1021
BspCNI CTCAG 4 cut(s) 346, 585, 930, 969
BspLI GGNNCC 1 cut(s) 904
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 1 cut(s) 576
BspTNI GGTCTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 2 cut(s) 442, 666
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 2 cut(s) 752, 915
BssMI GATC 2 cut(s) 382, 568
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 752
BstACI GRCGYC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 788
BstC8I GCNNGC 5 cut(s) 39, 46, 131, 222, 1041
BstDEI CTNAG 5 cut(s) 333, 572, 593, 938, 977
BstF5I GGATG 2 cut(s) 559, 851
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 2 cut(s) 385, 571
BstMAI GTCTC 2 cut(s) 243, 712
BstMBI GATC 2 cut(s) 382, 568
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 4 cut(s) 484, 515, 668, 771
BstNSI RCATGY 2 cut(s) 133, 697
BstSCI CCNGG 2 cut(s) 56, 914
BstSFI CTRYAG 2 cut(s) 324, 510
BstV1I GCAGC 7 cut(s) 28, 316, 465, 496, 502, 524, 695
BstX2I RGATCY 2 cut(s) 382, 568
BstYI RGATCY 2 cut(s) 382, 568
BstZI CGGCCG 1 cut(s) 46
BsuRI GGCC 4 cut(s) 48, 497, 757, 1021
BtsCI GGATG 2 cut(s) 559, 851
BtsIMutI CAGTG 2 cut(s) 580, 673
Cac8I GCNNGC 5 cut(s) 39, 46, 131, 222, 1041
CaiI CAGNNNCTG 1 cut(s) 689
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 806
CseI GACGC 2 cut(s) 61, 309
Csp6I GTAC 3 cut(s) 321, 648, 785
CviAII CATG 2 cut(s) 130, 694
CviQI GTAC 3 cut(s) 321, 648, 785
DdeI CTNAG 5 cut(s) 333, 572, 593, 938, 977
DpnI GATC 2 cut(s) 384, 570
DpnII GATC 2 cut(s) 382, 568
DraI TTTAAA 1 cut(s) 892
EaeI YGGCCR 1 cut(s) 46
EagI CGGCCG 1 cut(s) 46
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 2 cut(s) 942, 981
EclXI CGGCCG 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 752
Eco24I GRGCYC 2 cut(s) 944, 983
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 806
Eco52I CGGCCG 1 cut(s) 46
Eco53kI GAGCTC 2 cut(s) 942, 981
Eco57I CTGAAG 1 cut(s) 174
EcoICRI GAGCTC 2 cut(s) 942, 981
EcoRI GAATTC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 752
EcoT38I GRGCYC 2 cut(s) 944, 983
ErhI CCWWGG 1 cut(s) 752
FaeI CATG 2 cut(s) 133, 697
FaiI YATR 7 cut(s) 131, 213, 393, 423, 695, 960, 999
FalI AAGNNNNNCTT 4 cut(s) 369, 401, 868, 900
FatI CATG 2 cut(s) 129, 693
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 9 cut(s) 13, 42, 330, 479, 510, 513, 516, 684, 1037
FokI GGATG 2 cut(s) 566, 838
FriOI GRGCYC 2 cut(s) 944, 983
Fsp4HI GCNGC 9 cut(s) 13, 42, 330, 479, 510, 513, 516, 684, 1037
FspBI CTAG 5 cut(s) 519, 732, 753, 867, 897
GlaI GCGC 1 cut(s) 128
GluI GCNGC 9 cut(s) 13, 42, 330, 479, 510, 513, 516, 684, 1037
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 4 cut(s) 48, 497, 757, 1021
HapII CCGG 4 cut(s) 45, 58, 253, 915
HgaI GACGC 2 cut(s) 61, 309
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 2 cut(s) 133, 697
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 6 cut(s) 106, 112, 142, 189, 449, 801
HpaII CCGG 4 cut(s) 45, 58, 253, 915
HphI GGTGA 2 cut(s) 43, 455
Hpy166II GTNNAC 2 cut(s) 435, 849
Hpy188I TCNGA 7 cut(s) 111, 454, 717, 939, 952, 978, 991
Hpy188III TCNNGA 7 cut(s) 76, 103, 170, 193, 341, 386, 798
Hpy8I GTNNAC 2 cut(s) 435, 849
Hpy99I CGWCG 3 cut(s) 54, 77, 125
HpyAV CCTTC 3 cut(s) 179, 520, 819
HpyCH4IV ACGT 3 cut(s) 319, 787, 835
HpyCH4V TGCA 5 cut(s) 224, 347, 361, 512, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 484, 515, 668, 771
HpyF3I CTNAG 5 cut(s) 333, 572, 593, 938, 977
HpySE526I ACGT 3 cut(s) 319, 787, 835
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 2 cut(s) 133, 697
HspAI GCGC 1 cut(s) 127
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 382, 568
LmnI GCTCC 5 cut(s) 59, 122, 138, 947, 986
Lsp1109I GCAGC 7 cut(s) 28, 316, 465, 496, 502, 524, 695
LweI GCATC 5 cut(s) 334, 695, 732, 943, 982
MaeI CTAG 5 cut(s) 519, 732, 753, 867, 897
MaeII ACGT 3 cut(s) 319, 787, 835
MalI GATC 2 cut(s) 384, 570
MboI GATC 2 cut(s) 382, 568
MboII GAAGA 7 cut(s) 91, 268, 288, 303, 392, 548, 590
MflI RGATCY 2 cut(s) 382, 568
MhlI GDGCHC 3 cut(s) 793, 944, 983
MluCI AATT 5 cut(s) 263, 352, 499, 504, 766
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 3 cut(s) 393, 969, 1008
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 3 cut(s) 492, 503, 891
MspA1I CMGCKG 2 cut(s) 329, 515
MspI CCGG 4 cut(s) 45, 58, 253, 915
MspR9I CCNGG 2 cut(s) 58, 916
MwoI GCNNNNNNNGC 4 cut(s) 484, 515, 668, 771
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 2 cut(s) 58, 916
NdeII GATC 2 cut(s) 382, 568
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 2 cut(s) 133, 697
NlaIV GGNNCC 1 cut(s) 904
NmeAIII GCCGAG 1 cut(s) 196
NspI RCATGY 2 cut(s) 133, 697
PaeI GCATGC 1 cut(s) 133
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 5 cut(s) 106, 112, 142, 449, 801
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 9 cut(s) 14, 43, 331, 480, 511, 514, 517, 685, 1038
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Ppu21I YACGTR 1 cut(s) 788
Psp124BI GAGCTC 2 cut(s) 944, 983
PspN4I GGNNCC 1 cut(s) 904
PspPI GGNCC 1 cut(s) 806
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 382, 568
PvuII CAGCTG 2 cut(s) 329, 515
RsaI GTAC 3 cut(s) 322, 649, 786
RsaNI GTAC 3 cut(s) 321, 648, 785
SacI GAGCTC 2 cut(s) 944, 983
SaqAI TTAA 3 cut(s) 492, 503, 891
SatI GCNGC 9 cut(s) 13, 42, 330, 479, 510, 513, 516, 684, 1037
Sau3AI GATC 2 cut(s) 382, 568
Sau96I GGNCC 1 cut(s) 806
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 2 cut(s) 58, 916
SduI GDGCHC 3 cut(s) 793, 944, 983
SfaNI GCATC 5 cut(s) 334, 695, 732, 943, 982
SfcI CTRYAG 2 cut(s) 324, 510
SinI GGWCC 1 cut(s) 806
SmlI CTYRAG 5 cut(s) 101, 191, 604, 775, 907
SmoI CTYRAG 5 cut(s) 101, 191, 604, 775, 907
SphI GCATGC 1 cut(s) 133
Sse9I AATT 5 cut(s) 263, 352, 499, 504, 766
SsiI CCGC 5 cut(s) 12, 25, 93, 149, 597
SspI AATATT 1 cut(s) 240
SspMI CTAG 5 cut(s) 519, 732, 753, 867, 897
SstI GAGCTC 2 cut(s) 944, 983
StyD4I CCNGG 2 cut(s) 56, 914
StyI CCWWGG 1 cut(s) 752
TaiI ACGT 3 cut(s) 322, 790, 838
TaqI TCGA 2 cut(s) 75, 123
TaqII GACCGA 2 cut(s) 428, 794
TasI AATT 5 cut(s) 263, 352, 499, 504, 766
TatI WGTACW 1 cut(s) 647
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 5 cut(s) 106, 112, 142, 449, 801
Tru1I TTAA 3 cut(s) 492, 503, 891
Tru9I TTAA 3 cut(s) 492, 503, 891
TscAI CASTG 2 cut(s) 580, 673
TseI GCWGC 8 cut(s) 41, 329, 478, 509, 512, 515, 683, 1036
TspDTI ATGAA 5 cut(s) 200, 627, 645, 801, 816
TspGWI ACGGA 3 cut(s) 33, 109, 125
TspRI CASTG 2 cut(s) 580, 673
VpaK11BI GGWCC 1 cut(s) 806
XapI RAATTY 2 cut(s) 263, 352
XceI RCATGY 2 cut(s) 133, 697
XmaJI CCTAGG 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 848
XspI CTAG 5 cut(s) 519, 732, 753, 867, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.