Rroxscaffold_1G00037090

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008484
Physical Location & Seq
Forward (+)
54142958 .. 54148067
5110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00037090.1

Sequence Viewer

Length: 1002 bp
ATGGCTCACGGTGGCTACGGAAAGCGGAGGGTGAAGCCTGCTGCCGGCCGTCGCTCCCGGACGGGCTTGGGCGTCGAGAAGAAGCCCAAGTCCGCCTCCCTCAAGAATCAGATTCGCTCCGTCGAGCGCATGCTCCGTAAGAATCTACCGCCTGAAGTTAGGGAGGTTCAGGAAAAGAAGTTAGAAGGACTCAAGAAACAGCAAGATATTCATACTCGGCTTGCAGTAGAACGCAAAATATTTTTGCGAGACCGGAAGATAAAATTTTTTGAGAGAAGAAAGATAGAGAGAAGAATAAGACGCTTGGAGAAACTTCAACGTACTACAGCTGCTCAGGCTCAAGATGCAGAGAATTCTGTGCAGCTTTCAAAATTGAAAGAAGATCTTGAATATGTTAGGTTCTTTCCCAAGACCGAGAAATATGTCGCTCTGTTTACTGGAGGTGATGATTCAGAGCTTGTTGATCGGAGAAATAGGCTGCGTAAGCAGATTAAGGCCAATTTAATTGCTGCAGCTGCTAGTGGGAAGGATTTGGAAGAAACAGGGAGTGAGGATGATGGGCTTTTGGATCTCAGTGAAGATGATTTCTTTCTAAACGGAAGCTCAAGTGATGAAGCAAATGCAGATGATGAATGGACAGATAAGAGTACAAGGGAACAGGCTTCCAGTGCTTCTGGGAAAGCAGCATCTGGCGTGTCCAGTGATGAGAGAAATCAGATTTCAGCTAGAGCTTTGATGCCTCCCCCTAGGCCATCAAGTAATTCTCGCTCAAGTTCAGTACGTGCTCAATCAAGATTCGGTCCTTCATCAAGCAAAAGTTCATCAAAACGTGCTGAAATGTCTTCATCCAGCAATACATCAAGTAGCAGAAGTGGAACTTATTTTAAAGCTAGGGGTTCCTCAAGTTCAGGGGCAACTCAAAGTAGTAATCTGAGCTCCAACTCTGATGCTCATAAACCCCGTAGAAAGAGGAGGCCAAAAAAGAAAAAGCAGCAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Efg1

Protein Analysis

333

Amino Acids

37.18

Weight (kDa)

10.44

Isoelectric Point (pI)

63.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Efg1 PF10153 34 - 145 1.1e-29 rRNA-processing protein Efg1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 25, 93, 149
AclWI GGATC 1 cut(s) 576
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 263, 352
AcuI CTGAAG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 322, 649, 780
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 4 cut(s) 317, 369, 376, 389
AluBI AGCT 9 cut(s) 329, 364, 457, 515, 603, 725, 731, 890, 936
AluI AGCT 9 cut(s) 329, 364, 457, 515, 603, 725, 731, 890, 936
Alw21I GWGCWC 2 cut(s) 787, 938
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 576
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 4 cut(s) 46, 495, 749, 974
ApeKI GCWGC 9 cut(s) 41, 329, 361, 478, 509, 512, 515, 683, 991
ApoI RAATTY 2 cut(s) 263, 352
AspA2I CCTAGG 1 cut(s) 746
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 800
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 43, 455
AvaII GGWCC 1 cut(s) 800
AvrII CCTAGG 1 cut(s) 746
BanII GRGCYC 1 cut(s) 938
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 2 cut(s) 787, 938
BbvI GCAGC 8 cut(s) 28, 316, 373, 465, 496, 502, 524, 695
BccI CCATC 2 cut(s) 551, 760
BceAI ACGGC 1 cut(s) 33
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 4 cut(s) 519, 726, 747, 891
BfmI CTRYAG 2 cut(s) 324, 510
BglII AGATCT 1 cut(s) 382
BisI GCNGC 9 cut(s) 42, 330, 362, 479, 510, 513, 516, 684, 992
BlnI CCTAGG 1 cut(s) 746
BlsI GCNGC 9 cut(s) 43, 331, 363, 480, 511, 514, 517, 685, 993
Bme1390I CCNGG 1 cut(s) 58
Bme18I GGWCC 1 cut(s) 800
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 1 cut(s) 898
BmrFI CCNGG 1 cut(s) 58
BmsI GCATC 4 cut(s) 334, 695, 726, 937
BpiI GAAGAC 1 cut(s) 834
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 6 cut(s) 86, 176, 324, 589, 754, 886
BpuMI CCSGG 1 cut(s) 58
BsaAI YACGTR 1 cut(s) 782
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 746
BsaWI WCCGGW 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 3 cut(s) 442, 666, 699
BseDI CCNNGG 1 cut(s) 746
BseGI GGATG 2 cut(s) 559, 845
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 3 cut(s) 347, 586, 923
BseNI ACTGG 3 cut(s) 442, 666, 699
BseRI GAGGAG 1 cut(s) 985
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 8 cut(s) 28, 316, 373, 465, 496, 502, 524, 695
BsgI GTGCAG 1 cut(s) 380
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 4 cut(s) 48, 497, 751, 976
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 2 cut(s) 787, 938
BsiSI CCGG 3 cut(s) 45, 58, 253
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 243
BsnI GGCC 4 cut(s) 48, 497, 751, 976
Bso31I GGTCTC 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 787, 938
Bsp143I GATC 3 cut(s) 382, 463, 568
BspACI CCGC 3 cut(s) 25, 93, 149
BspANI GGCC 4 cut(s) 48, 497, 751, 976
BspCNI CTCAG 3 cut(s) 346, 585, 924
BspLI GGNNCC 1 cut(s) 898
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 1 cut(s) 576
BspTNI GGTCTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 3 cut(s) 442, 666, 699
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 746
BssMI GATC 3 cut(s) 382, 463, 568
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 746
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 782
BstC8I GCNNGC 5 cut(s) 39, 46, 131, 222, 996
BstDEI CTNAG 3 cut(s) 333, 572, 932
BstF5I GGATG 2 cut(s) 559, 845
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 3 cut(s) 385, 466, 571
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 3 cut(s) 382, 463, 568
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 5 cut(s) 335, 344, 484, 515, 668
BstNSI RCATGY 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 56
BstSFI CTRYAG 2 cut(s) 324, 510
BstV1I GCAGC 8 cut(s) 28, 316, 373, 465, 496, 502, 524, 695
BstV2I GAAGAC 1 cut(s) 834
BstX2I RGATCY 2 cut(s) 382, 568
BstYI RGATCY 2 cut(s) 382, 568
BstZI CGGCCG 1 cut(s) 46
BsuRI GGCC 4 cut(s) 48, 497, 751, 976
BtsCI GGATG 2 cut(s) 559, 845
BtsIMutI CAGTG 3 cut(s) 580, 673, 706
Cac8I GCNNGC 5 cut(s) 39, 46, 131, 222, 996
CaiI CAGNNNCTG 1 cut(s) 689
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 800
CseI GACGC 2 cut(s) 61, 309
Csp6I GTAC 3 cut(s) 321, 648, 779
CviAII CATG 2 cut(s) 130, 999
CviQI GTAC 3 cut(s) 321, 648, 779
DdeI CTNAG 3 cut(s) 333, 572, 932
DpnI GATC 3 cut(s) 384, 465, 570
DpnII GATC 3 cut(s) 382, 463, 568
DraI TTTAAA 1 cut(s) 886
EaeI YGGCCR 1 cut(s) 46
EagI CGGCCG 1 cut(s) 46
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 936
EclXI CGGCCG 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 746
Eco24I GRGCYC 1 cut(s) 938
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 800
Eco52I CGGCCG 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 936
Eco57I CTGAAG 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 936
EcoRI GAATTC 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 746
EcoT38I GRGCYC 1 cut(s) 938
ErhI CCWWGG 1 cut(s) 746
FaeI CATG 2 cut(s) 133, 1002
FaiI YATR 6 cut(s) 131, 213, 393, 423, 954, 1000
FalI AAGNNNNNCTT 4 cut(s) 369, 401, 862, 894
FatI CATG 2 cut(s) 129, 998
Fnu4HI GCNGC 9 cut(s) 42, 330, 362, 479, 510, 513, 516, 684, 992
FokI GGATG 2 cut(s) 566, 832
FriOI GRGCYC 1 cut(s) 938
Fsp4HI GCNGC 9 cut(s) 42, 330, 362, 479, 510, 513, 516, 684, 992
FspBI CTAG 4 cut(s) 519, 726, 747, 891
GlaI GCGC 1 cut(s) 128
GluI GCNGC 9 cut(s) 42, 330, 362, 479, 510, 513, 516, 684, 992
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 4 cut(s) 48, 497, 751, 976
HapII CCGG 3 cut(s) 45, 58, 253
HgaI GACGC 2 cut(s) 61, 309
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 2 cut(s) 133, 1002
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 6 cut(s) 106, 112, 142, 189, 449, 795
HpaII CCGG 3 cut(s) 45, 58, 253
HphI GGTGA 2 cut(s) 43, 455
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 6 cut(s) 111, 454, 468, 717, 933, 946
Hpy188III TCNNGA 7 cut(s) 76, 103, 170, 193, 341, 386, 792
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 3 cut(s) 54, 77, 125
HpyAV CCTTC 3 cut(s) 179, 520, 813
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 3 cut(s) 319, 781, 829
HpyCH4V TGCA 5 cut(s) 224, 347, 361, 512, 623
HpyF10VI GCNNNNNNNGC 5 cut(s) 335, 344, 484, 515, 668
HpyF3I CTNAG 3 cut(s) 333, 572, 932
HpySE526I ACGT 3 cut(s) 319, 781, 829
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 2 cut(s) 133, 1002
HspAI GCGC 1 cut(s) 127
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Kzo9I GATC 3 cut(s) 382, 463, 568
LmnI GCTCC 4 cut(s) 59, 122, 138, 941
Lsp1109I GCAGC 8 cut(s) 28, 316, 373, 465, 496, 502, 524, 695
LweI GCATC 4 cut(s) 334, 695, 726, 937
MaeI CTAG 4 cut(s) 519, 726, 747, 891
MaeII ACGT 3 cut(s) 319, 781, 829
MalI GATC 3 cut(s) 384, 465, 570
MboI GATC 3 cut(s) 382, 463, 568
MboII GAAGA 8 cut(s) 91, 268, 288, 303, 392, 548, 590, 834
MflI RGATCY 2 cut(s) 382, 568
MhlI GDGCHC 2 cut(s) 787, 938
MluCI AATT 6 cut(s) 263, 352, 371, 499, 504, 760
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 963
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 3 cut(s) 492, 503, 885
MspA1I CMGCKG 2 cut(s) 329, 515
MspI CCGG 3 cut(s) 45, 58, 253
MspR9I CCNGG 1 cut(s) 58
MwoI GCNNNNNNNGC 5 cut(s) 335, 344, 484, 515, 668
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 1 cut(s) 58
NdeII GATC 3 cut(s) 382, 463, 568
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 2 cut(s) 133, 1002
NlaIV GGNNCC 1 cut(s) 898
NmeAIII GCCGAG 1 cut(s) 196
NspI RCATGY 1 cut(s) 133
PaeI GCATGC 1 cut(s) 133
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 5 cut(s) 106, 112, 142, 449, 795
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 9 cut(s) 43, 331, 363, 480, 511, 514, 517, 685, 993
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Ppu21I YACGTR 1 cut(s) 782
Psp124BI GAGCTC 1 cut(s) 938
PspN4I GGNNCC 1 cut(s) 898
PspPI GGNCC 1 cut(s) 800
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 382, 568
PvuII CAGCTG 2 cut(s) 329, 515
RsaI GTAC 3 cut(s) 322, 649, 780
RsaNI GTAC 3 cut(s) 321, 648, 779
SacI GAGCTC 1 cut(s) 938
SaqAI TTAA 3 cut(s) 492, 503, 885
SatI GCNGC 9 cut(s) 42, 330, 362, 479, 510, 513, 516, 684, 992
Sau3AI GATC 3 cut(s) 382, 463, 568
Sau96I GGNCC 1 cut(s) 800
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 58
SduI GDGCHC 2 cut(s) 787, 938
SfaNI GCATC 4 cut(s) 334, 695, 726, 937
SfcI CTRYAG 2 cut(s) 324, 510
SinI GGWCC 1 cut(s) 800
SmlI CTYRAG 6 cut(s) 101, 191, 339, 604, 769, 901
SmoI CTYRAG 6 cut(s) 101, 191, 339, 604, 769, 901
SphI GCATGC 1 cut(s) 133
Sse9I AATT 6 cut(s) 263, 352, 371, 499, 504, 760
SsiI CCGC 3 cut(s) 25, 93, 149
SspI AATATT 1 cut(s) 240
SspMI CTAG 4 cut(s) 519, 726, 747, 891
SstI GAGCTC 1 cut(s) 938
StyD4I CCNGG 1 cut(s) 56
StyI CCWWGG 1 cut(s) 746
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 3 cut(s) 322, 784, 832
TaqI TCGA 2 cut(s) 75, 123
TaqII GACCGA 2 cut(s) 428, 788
TasI AATT 6 cut(s) 263, 352, 371, 499, 504, 760
TatI WGTACW 1 cut(s) 647
TfiI GAWTC 5 cut(s) 106, 112, 142, 449, 795
Tru1I TTAA 3 cut(s) 492, 503, 885
Tru9I TTAA 3 cut(s) 492, 503, 885
TscAI CASTG 3 cut(s) 580, 673, 706
TseI GCWGC 9 cut(s) 41, 329, 361, 478, 509, 512, 515, 683, 991
TspDTI ATGAA 6 cut(s) 200, 627, 645, 795, 810, 834
TspGWI ACGGA 4 cut(s) 33, 109, 125, 612
TspRI CASTG 3 cut(s) 580, 673, 706
VpaK11BI GGWCC 1 cut(s) 800
XapI RAATTY 2 cut(s) 263, 352
XceI RCATGY 1 cut(s) 133
XmaJI CCTAGG 1 cut(s) 746
XspI CTAG 4 cut(s) 519, 726, 747, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.