MD16G1206200.v1.1

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008484
Physical Location & Seq
Forward (+)
19071625 .. 19076064
4440 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1206200.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCTCACGGTGGCCACAGCAAGCGGCGGGTGAACCCCGCCGGACATCGGTCCAAGTCGGCCTTGGGCCCGGAGAAGAAGCCCAAGTCCGTCACCCTCAAAAACCAGATTCGCTCCATCGAGCGCATGCTCCGTAAGAATCTACCAGCTGAAGTTAGGGAGGCTCAGGAAAAGAAGTTGGAAGGATTCAAGAAACAACAGGAGATTCACTCCCGTCTCGCTGTGGAACGCAAAATATTTATGCGTGACAGGAAGATAAAGTTTTTTGAGAGAAGAAAGATAGAGAGAAGAATAAGACGCCTGGAGAAACTCCAACGTGCTTCATCGGGTCAGGCACAAGACGCAGAGGTTTCTGTGAACCTTTCGAAATTGAAAGAAGATCTTGAATATGTTAGGTTCTTTCCCAAGACTGAAAAGTACGTCTCTCTATTTACTGGAGGTGAAGATACAGGTGTGATTGATACGAGAAATAGGTTGCGAAAGCAGATTAAGGCCAATATAGTTGCTGCAGCAGCTAGCGGAAAGGATTTGGAAGAAACAGGGAGTGAGGACGATGGGCTTGTGGATATGAGCGAAGATGATTTCTTCCTAAATGGGAGTTCTAGTGATGAAGCAGATGCAGACGATGAATGGACAGATAAGAGTACAAAGGAACAGGTGTCCAGTGCTTCAGGAAAAGCAACTTCTGGCATGTCCAGTGATGAGAGAAATCAGCGACAGATTTCTGCTAGAGCTTTGATGCCTCCTCCCCGGCCATCCAGCAATTCTCACCCAAGTTCTGTTCGTGCTCAGTCAAGGTTTGGTCCTTCATCAAGCAAAAATTCCTCAAAAAGCATCAGTGAAATGGCCACATCAAGCGCTACATCAAATAGCAGAAGTGGAACTTCCATCAATACATCAAGTGGCAGAAGTGGAACTTCGTTCAAAGCTAGGGTATCCTCTAATTCAACTGCAGGTCAAAGTAGTAATTTGAGCTCCAACTCTGATGCTCATAAACCCCGTAGAAAGAGGAGGCCAAAGAAGAAAAAGCAGCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Efg1

Protein Analysis

347

Amino Acids

38.37

Weight (kDa)

10.33

Isoelectric Point (pI)

58.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Efg1 PF10153 33 - 144 5.2e-31 rRNA-processing protein Efg1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 944
AciI CCGC 4 cut(s) 25, 28, 39, 519
AcoI YGGCCR 3 cut(s) 13, 752, 846
AcsI RAATTY 1 cut(s) 820
AcuI CTGAAG 2 cut(s) 171, 654
AcyI GRCGYC 1 cut(s) 298
AfaI GTAC 2 cut(s) 419, 646
AfeI AGCGCT 1 cut(s) 859
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 5 cut(s) 190, 373, 386, 925, 948
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 5 cut(s) 149, 515, 734, 929, 975
AluI AGCT 5 cut(s) 149, 515, 734, 929, 975
Alw21I GWGCWC 2 cut(s) 790, 977
Alw26I GTCTC 2 cut(s) 221, 427
Aor51HI AGCGCT 1 cut(s) 859
AoxI GGCC 7 cut(s) 13, 60, 67, 492, 752, 846, 1013
ApaI GGGCCC 1 cut(s) 71
ApeKI GCWGC 4 cut(s) 506, 509, 512, 1030
ApoI RAATTY 1 cut(s) 820
AspLEI GCGC 2 cut(s) 126, 860
AspS9I GGNCC 4 cut(s) 51, 67, 68, 803
AsuC2I CCSGG 2 cut(s) 71, 751
AsuHPI GGTGA 4 cut(s) 43, 85, 452, 761
AsuII TTCGAA 1 cut(s) 365
AsuNHI GCTAGC 1 cut(s) 515
AvaII GGWCC 2 cut(s) 51, 803
BaeGI GKGCMC 1 cut(s) 71
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BalI TGGCCA 2 cut(s) 15, 848
BanII GRGCYC 2 cut(s) 71, 977
Bbv12I GWGCWC 2 cut(s) 790, 977
BbvI GCAGC 3 cut(s) 493, 521, 524
BccI CCATC 4 cut(s) 125, 548, 763, 896
BciT130I CCWGG 1 cut(s) 302
BciVI GTATCC 1 cut(s) 946
BcnI CCSGG 2 cut(s) 71, 751
BcoDI GTCTC 2 cut(s) 221, 427
BfaI CTAG 4 cut(s) 516, 603, 729, 930
BfmI CTRYAG 2 cut(s) 507, 951
BfoI RGCGCY 1 cut(s) 861
BfuAI ACCTGC 1 cut(s) 944
BfuI GTATCC 1 cut(s) 946
BglII AGATCT 1 cut(s) 379
BisI GCNGC 5 cut(s) 26, 507, 510, 513, 1031
BlsI GCNGC 5 cut(s) 27, 508, 511, 514, 1032
Bme1390I CCNGG 3 cut(s) 71, 302, 751
Bme18I GGWCC 2 cut(s) 51, 803
BmgT120I GGNCC 4 cut(s) 51, 67, 68, 803
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 3 cut(s) 71, 302, 751
BmsI GCATC 4 cut(s) 607, 729, 843, 976
BmtI GCTAGC 1 cut(s) 519
BoxI GACNNNNGTC 1 cut(s) 49
BplI GAGNNNNNCTC 2 cut(s) 194, 226
BpmI CTGGAG 2 cut(s) 323, 456
Bpu10I CCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 365
BpuMI CCSGG 2 cut(s) 71, 751
BsaHI GRCGYC 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 63, 749
BsaXI ACNNNNNCTCC 2 cut(s) 589, 619
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 3 cut(s) 439, 663, 696
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 2 cut(s) 63, 749
BseGI GGATG 1 cut(s) 755
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMII CTCAG 2 cut(s) 179, 803
BseNI ACTGG 3 cut(s) 439, 663, 696
BseRI GAGGAG 2 cut(s) 735, 1024
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 3 cut(s) 493, 521, 524
BshFI GGCC 7 cut(s) 15, 62, 69, 494, 754, 848, 1015
BsiHKAI GWGCWC 2 cut(s) 790, 977
BsiSI CCGG 3 cut(s) 42, 71, 751
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 2 cut(s) 221, 427
BsmBI CGTCTC 2 cut(s) 221, 427
BsnI GGCC 7 cut(s) 15, 62, 69, 494, 754, 848, 1015
Bsp119I TTCGAA 1 cut(s) 365
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 3 cut(s) 71, 790, 977
Bsp143I GATC 1 cut(s) 379
BspACI CCGC 4 cut(s) 25, 28, 39, 519
BspANI GGCC 7 cut(s) 15, 62, 69, 494, 754, 848, 1015
BspCNI CTCAG 2 cut(s) 178, 802
BspLI GGNNCC 1 cut(s) 69
BspMAI CTGCAG 2 cut(s) 511, 955
BspMI ACCTGC 1 cut(s) 944
BspOI GCTAGC 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 365
BsrI ACTGG 3 cut(s) 439, 663, 696
BssECI CCNNGG 2 cut(s) 63, 749
BssMI GATC 1 cut(s) 379
BssNI GRCGYC 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 63
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 1 cut(s) 298
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 3 cut(s) 23, 128, 517
BstDEI CTNAG 2 cut(s) 165, 789
BstF5I GGATG 1 cut(s) 755
BstH2I RGCGCY 1 cut(s) 861
BstHHI GCGC 2 cut(s) 126, 860
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 2 cut(s) 221, 427
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 2 cut(s) 341, 512
BstNI CCWGG 1 cut(s) 302
BstNSI RCATGY 2 cut(s) 130, 694
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 3 cut(s) 69, 300, 749
BstSFI CTRYAG 2 cut(s) 507, 951
BstSLI GKGCMC 1 cut(s) 71
BstV1I GCAGC 3 cut(s) 493, 521, 524
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuI GTATCC 1 cut(s) 946
BsuRI GGCC 7 cut(s) 15, 62, 69, 494, 754, 848, 1015
BtsCI GGATG 1 cut(s) 755
BtsIMutI CAGTG 3 cut(s) 670, 703, 844
BveI ACCTGC 1 cut(s) 944
Cac8I GCNNGC 3 cut(s) 23, 128, 517
CfoI GCGC 2 cut(s) 126, 860
Cfr13I GGNCC 4 cut(s) 51, 67, 68, 803
CseI GACGC 2 cut(s) 306, 350
Csp6I GTAC 2 cut(s) 418, 645
CviAII CATG 2 cut(s) 127, 691
CviQI GTAC 2 cut(s) 418, 645
DdeI CTNAG 2 cut(s) 165, 789
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
EaeI YGGCCR 3 cut(s) 13, 752, 846
Ecl136II GAGCTC 1 cut(s) 975
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 2 cut(s) 71, 977
Eco47I GGWCC 2 cut(s) 51, 803
Eco47III AGCGCT 1 cut(s) 859
Eco53kI GAGCTC 1 cut(s) 975
Eco57I CTGAAG 2 cut(s) 171, 654
EcoICRI GAGCTC 1 cut(s) 975
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 2 cut(s) 71, 977
ErhI CCWWGG 1 cut(s) 63
Esp3I CGTCTC 2 cut(s) 221, 427
FaeI CATG 2 cut(s) 130, 694
FaiI YATR 7 cut(s) 128, 242, 390, 500, 569, 692, 993
FalI AAGNNNNNCTT 8 cut(s) 47, 79, 366, 398, 868, 900, 901, 933
FatI CATG 2 cut(s) 126, 690
FauI CCCGC 2 cut(s) 21, 46
Fnu4HI GCNGC 5 cut(s) 26, 507, 510, 513, 1031
FokI GGATG 1 cut(s) 742
FriOI GRGCYC 2 cut(s) 71, 977
Fsp4HI GCNGC 5 cut(s) 26, 507, 510, 513, 1031
FspBI CTAG 4 cut(s) 516, 603, 729, 930
GlaI GCGC 2 cut(s) 125, 859
GluI GCNGC 5 cut(s) 26, 507, 510, 513, 1031
GsuI CTGGAG 2 cut(s) 323, 456
HaeII RGCGCY 1 cut(s) 861
HaeIII GGCC 7 cut(s) 15, 62, 69, 494, 754, 848, 1015
HapII CCGG 3 cut(s) 42, 71, 751
HgaI GACGC 2 cut(s) 306, 350
HhaI GCGC 2 cut(s) 126, 860
Hin1I GRCGYC 1 cut(s) 298
Hin1II CATG 2 cut(s) 130, 694
Hin6I GCGC 2 cut(s) 124, 858
HinP1I GCGC 2 cut(s) 124, 858
HinfI GANTC 4 cut(s) 109, 139, 186, 205
HpaII CCGG 3 cut(s) 42, 71, 751
HphI GGTGA 4 cut(s) 43, 85, 452, 761
Hpy166II GTNNAC 2 cut(s) 34, 358
Hpy188I TCNGA 1 cut(s) 985
Hpy188III TCNNGA 4 cut(s) 167, 190, 383, 672
Hpy8I GTNNAC 2 cut(s) 34, 358
HpyAV CCTTC 2 cut(s) 176, 816
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 2 cut(s) 316, 420
HpyCH4V TGCA 3 cut(s) 509, 620, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 512
HpyF3I CTNAG 2 cut(s) 165, 789
HpySE526I ACGT 2 cut(s) 316, 420
Hsp92I GRCGYC 1 cut(s) 298
Hsp92II CATG 2 cut(s) 130, 694
HspAI GCGC 2 cut(s) 124, 858
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 3 cut(s) 119, 135, 980
Lsp1109I GCAGC 3 cut(s) 493, 521, 524
LweI GCATC 4 cut(s) 607, 729, 843, 976
MaeI CTAG 4 cut(s) 516, 603, 729, 930
MaeII ACGT 2 cut(s) 316, 420
MaeIII GTNAC 2 cut(s) 91, 245
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 3 cut(s) 71, 790, 977
MlsI TGGCCA 2 cut(s) 15, 848
MluCI AATT 5 cut(s) 368, 763, 820, 943, 967
MluNI TGGCCA 2 cut(s) 15, 848
MmeI TCCRAC 3 cut(s) 159, 337, 1002
Mox20I TGGCCA 2 cut(s) 15, 848
MscI TGGCCA 2 cut(s) 15, 848
MseI TTAA 1 cut(s) 489
Msp20I TGGCCA 2 cut(s) 15, 848
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 3 cut(s) 42, 71, 751
MspR9I CCNGG 3 cut(s) 71, 302, 751
MvaI CCWGG 1 cut(s) 302
MwoI GCNNNNNNNGC 2 cut(s) 341, 512
NciI CCSGG 2 cut(s) 71, 751
NdeII GATC 1 cut(s) 379
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 2 cut(s) 130, 694
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 2 cut(s) 91, 245
NspI RCATGY 2 cut(s) 130, 694
NspV TTCGAA 1 cut(s) 365
PaeI GCATGC 1 cut(s) 130
PfeI GAWTC 4 cut(s) 109, 139, 186, 205
PkrI GCNGC 5 cut(s) 27, 508, 511, 514, 1032
PshAI GACNNNNGTC 1 cut(s) 49
Psp124BI GAGCTC 1 cut(s) 977
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 69
PspOMI GGGCCC 1 cut(s) 67
PspPI GGNCC 4 cut(s) 51, 67, 68, 803
PstI CTGCAG 2 cut(s) 511, 955
PsuI RGATCY 1 cut(s) 379
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 2 cut(s) 419, 646
RsaNI GTAC 2 cut(s) 418, 645
SacI GAGCTC 1 cut(s) 977
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 5 cut(s) 26, 507, 510, 513, 1031
Sau3AI GATC 1 cut(s) 379
Sau96I GGNCC 4 cut(s) 51, 67, 68, 803
ScrFI CCNGG 3 cut(s) 71, 302, 751
SduI GDGCHC 3 cut(s) 71, 790, 977
SfaNI GCATC 4 cut(s) 607, 729, 843, 976
SfcI CTRYAG 2 cut(s) 507, 951
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 2 cut(s) 51, 803
SphI GCATGC 1 cut(s) 130
Sse9I AATT 5 cut(s) 368, 763, 820, 943, 967
SsiI CCGC 4 cut(s) 25, 28, 39, 519
SspI AATATT 1 cut(s) 237
SspMI CTAG 4 cut(s) 516, 603, 729, 930
SstI GAGCTC 1 cut(s) 977
StyD4I CCNGG 3 cut(s) 69, 300, 749
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 2 cut(s) 319, 423
TaqI TCGA 2 cut(s) 120, 365
TaqII GACCGA 1 cut(s) 39
TasI AATT 5 cut(s) 368, 763, 820, 943, 967
TatI WGTACW 1 cut(s) 644
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 4 cut(s) 109, 139, 186, 205
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 3 cut(s) 670, 703, 844
TseFI GTSAC 2 cut(s) 91, 245
TseI GCWGC 4 cut(s) 506, 509, 512, 1030
Tsp45I GTSAC 2 cut(s) 91, 245
TspDTI ATGAA 4 cut(s) 312, 624, 642, 798
TspGWI ACGGA 2 cut(s) 79, 122
TspRI CASTG 3 cut(s) 670, 703, 844
VpaK11BI GGWCC 2 cut(s) 51, 803
XapI RAATTY 1 cut(s) 820
XceI RCATGY 2 cut(s) 130, 694
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XspI CTAG 4 cut(s) 516, 603, 729, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.