RchiOBHm_Chr5g0043971

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008484
Physical Location & Seq
Reverse (-)
39027662 .. 39033407
5746 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32220

Sequence Viewer

Length: 1008 bp
ATGGCTCACGGCGGCTACGGAAAGCGGAGGGTGAAGCCTGCTGCCGGCCGTCGCTCCCGGACGGGCTTGGGCGTCGAGAAGAAGCCCAAGTCCGCCTCCCTCAAGAATCAGATTCGCTCCGTCGAGCGCATGCTCCGTAAGAATCTACCGCCTGAAGTTAGGGAGGTTCAGGAAAAGAAGTTAGAAGGACTCAAGAAACAGCAAGATATTCATACTCGGCTTGCAGTAGAACGCAAAATATTTTTGCGAGACCGGAAGATAAAATTTTTTGAGAGAAGAAAGATAGAGAGAAGAATAAGACGCTTGGAGAAACTTCAACGTACTACAGCTGCTCAGGCGCAAGATGCAGAGAATTCTGTGCAACTTTCCAAATTGAAAGAAGATCTTGAATATGTTAGGTTCTTTCCCAAGACCGAGAAATATGTCGCTCTGTTTACTGGAGGTGATGATTCAGAGCTTGTTGATAGGAGAAATAGGATGCGTAAGCAGATTAAGGCCAATTTAATTGCTGCAGCTGCTAGTGGGAAGGATTTGGAAGAAACAGGGAGTGAGGATGATGGGCTTTTGGATCTCAGTGAAGATGATTTCTTTCTAAGTGGAAGCTCAAGTGATGAAGCAAATGCAGATGATGAATGGACAGATAAGAGTACAAGGGAACAGGCTTCCAGTGCTTCTGGGAAAGCAGCATCTGGCATGTCCAGTGATGAGAGAAATCAGAGACAGATTTCAGCTAGAGCTTTGATGCCTCCCCCTAGGCCATCAAGTAATTCTCGCTCAAGTTCAGTACGTGCTCAATCAAGATTCGGTCCTTCATCAAGCAAAAGTTCATCAAAACGTGCTGAAATGTCTACATCCAGCAATACATCAAGTAGCAGAAGTGGAACTTATTTTAAACCTAGGGGTTCCTCAAGTTCAGGGGCAACTCAAATTAGTAATCTGAGCTCCAACTCTGATGCTCATAAACCCCGTAGAAAGAGGAGGCCAAAAAAGAAAAAGCAGCAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Efg1

Protein Analysis

335

Amino Acids

37.55

Weight (kDa)

10.49

Isoelectric Point (pI)

61.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Efg1 PF10153 34 - 145 1.1e-29 rRNA-processing protein Efg1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 848
AciI CCGC 4 cut(s) 12, 25, 93, 149
AclWI GGATC 1 cut(s) 576
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 263, 352
AcuI CTGAAG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 322, 649, 786
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 3 cut(s) 317, 376, 389
AluBI AGCT 7 cut(s) 329, 457, 515, 603, 731, 737, 942
AluI AGCT 7 cut(s) 329, 457, 515, 603, 731, 737, 942
Alw21I GWGCWC 2 cut(s) 793, 944
Alw26I GTCTC 2 cut(s) 243, 712
AlwI GGATC 1 cut(s) 576
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 4 cut(s) 46, 495, 755, 980
ApeKI GCWGC 7 cut(s) 41, 329, 509, 512, 515, 683, 997
ApoI RAATTY 2 cut(s) 263, 352
AspA2I CCTAGG 2 cut(s) 752, 896
AspLEI GCGC 2 cut(s) 129, 340
AspS9I GGNCC 1 cut(s) 806
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 43, 455
AvaII GGWCC 1 cut(s) 806
AvrII CCTAGG 2 cut(s) 752, 896
BanII GRGCYC 1 cut(s) 944
Bbv12I GWGCWC 2 cut(s) 793, 944
BbvI GCAGC 6 cut(s) 28, 316, 496, 502, 524, 695
BccI CCATC 2 cut(s) 551, 766
BceAI ACGGC 2 cut(s) 25, 33
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 2 cut(s) 243, 712
BfaI CTAG 4 cut(s) 519, 732, 753, 897
BfmI CTRYAG 2 cut(s) 324, 510
BglII AGATCT 1 cut(s) 382
BisI GCNGC 8 cut(s) 13, 42, 330, 510, 513, 516, 684, 998
BlnI CCTAGG 2 cut(s) 752, 896
BlsI GCNGC 8 cut(s) 14, 43, 331, 511, 514, 517, 685, 999
Bme1390I CCNGG 1 cut(s) 58
Bme18I GGWCC 1 cut(s) 806
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 1 cut(s) 904
BmrFI CCNGG 1 cut(s) 58
BmsI GCATC 5 cut(s) 334, 468, 695, 732, 943
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 5 cut(s) 86, 176, 589, 760, 892
BpuMI CCSGG 1 cut(s) 58
BsaAI YACGTR 1 cut(s) 788
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 2 cut(s) 752, 896
BsaWI WCCGGW 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 3 cut(s) 442, 666, 699
BseDI CCNNGG 2 cut(s) 752, 896
BseGI GGATG 3 cut(s) 483, 559, 851
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 3 cut(s) 347, 586, 929
BseNI ACTGG 3 cut(s) 442, 666, 699
BseRI GAGGAG 1 cut(s) 991
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 6 cut(s) 28, 316, 496, 502, 524, 695
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 4 cut(s) 48, 497, 757, 982
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 2 cut(s) 793, 944
BsiSI CCGG 3 cut(s) 45, 58, 253
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 2 cut(s) 243, 712
BsnI GGCC 4 cut(s) 48, 497, 757, 982
Bso31I GGTCTC 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 793, 944
Bsp143I GATC 2 cut(s) 382, 568
BspACI CCGC 4 cut(s) 12, 25, 93, 149
BspANI GGCC 4 cut(s) 48, 497, 757, 982
BspCNI CTCAG 3 cut(s) 346, 585, 930
BspLI GGNNCC 1 cut(s) 904
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 1 cut(s) 576
BspTNI GGTCTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 3 cut(s) 442, 666, 699
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 2 cut(s) 752, 896
BssMI GATC 2 cut(s) 382, 568
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 752, 896
BstACI GRCGYC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 788
BstC8I GCNNGC 5 cut(s) 39, 46, 131, 222, 1002
BstDEI CTNAG 4 cut(s) 333, 572, 593, 938
BstF5I GGATG 3 cut(s) 483, 559, 851
BstHHI GCGC 2 cut(s) 129, 340
BstKTI GATC 2 cut(s) 385, 571
BstMAI GTCTC 2 cut(s) 243, 712
BstMBI GATC 2 cut(s) 382, 568
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 4 cut(s) 335, 344, 515, 668
BstNSI RCATGY 2 cut(s) 133, 697
BstSCI CCNGG 1 cut(s) 56
BstSFI CTRYAG 2 cut(s) 324, 510
BstV1I GCAGC 6 cut(s) 28, 316, 496, 502, 524, 695
BstX2I RGATCY 2 cut(s) 382, 568
BstYI RGATCY 2 cut(s) 382, 568
BstZI CGGCCG 1 cut(s) 46
BsuRI GGCC 4 cut(s) 48, 497, 757, 982
BtsCI GGATG 3 cut(s) 483, 559, 851
BtsIMutI CAGTG 3 cut(s) 580, 673, 706
Cac8I GCNNGC 5 cut(s) 39, 46, 131, 222, 1002
CaiI CAGNNNCTG 1 cut(s) 689
CfoI GCGC 2 cut(s) 129, 340
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 806
CseI GACGC 2 cut(s) 61, 309
Csp6I GTAC 3 cut(s) 321, 648, 785
CviAII CATG 3 cut(s) 130, 694, 1005
CviQI GTAC 3 cut(s) 321, 648, 785
DdeI CTNAG 4 cut(s) 333, 572, 593, 938
DpnI GATC 2 cut(s) 384, 570
DpnII GATC 2 cut(s) 382, 568
DraI TTTAAA 1 cut(s) 892
EaeI YGGCCR 1 cut(s) 46
EagI CGGCCG 1 cut(s) 46
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 942
EclXI CGGCCG 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 752, 896
Eco24I GRGCYC 1 cut(s) 944
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 806
Eco52I CGGCCG 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 942
Eco57I CTGAAG 1 cut(s) 174
EcoICRI GAGCTC 1 cut(s) 942
EcoRI GAATTC 1 cut(s) 352
EcoT14I CCWWGG 2 cut(s) 752, 896
EcoT38I GRGCYC 1 cut(s) 944
ErhI CCWWGG 2 cut(s) 752, 896
FaeI CATG 3 cut(s) 133, 697, 1008
FaiI YATR 7 cut(s) 131, 213, 393, 423, 695, 960, 1006
FalI AAGNNNNNCTT 4 cut(s) 369, 401, 868, 900
FatI CATG 3 cut(s) 129, 693, 1004
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 8 cut(s) 13, 42, 330, 510, 513, 516, 684, 998
FokI GGATG 3 cut(s) 490, 566, 838
FriOI GRGCYC 1 cut(s) 944
Fsp4HI GCNGC 8 cut(s) 13, 42, 330, 510, 513, 516, 684, 998
FspBI CTAG 4 cut(s) 519, 732, 753, 897
GlaI GCGC 2 cut(s) 128, 339
GluI GCNGC 8 cut(s) 13, 42, 330, 510, 513, 516, 684, 998
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 4 cut(s) 48, 497, 757, 982
HapII CCGG 3 cut(s) 45, 58, 253
HgaI GACGC 2 cut(s) 61, 309
HhaI GCGC 2 cut(s) 129, 340
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 3 cut(s) 133, 697, 1008
Hin6I GCGC 2 cut(s) 127, 338
HinP1I GCGC 2 cut(s) 127, 338
HinfI GANTC 6 cut(s) 106, 112, 142, 189, 449, 801
HpaII CCGG 3 cut(s) 45, 58, 253
HphI GGTGA 2 cut(s) 43, 455
Hpy166II GTNNAC 2 cut(s) 435, 849
Hpy188I TCNGA 5 cut(s) 111, 454, 717, 939, 952
Hpy188III TCNNGA 6 cut(s) 76, 103, 170, 193, 386, 798
Hpy8I GTNNAC 2 cut(s) 435, 849
Hpy99I CGWCG 3 cut(s) 54, 77, 125
HpyAV CCTTC 3 cut(s) 179, 520, 819
HpyCH4IV ACGT 3 cut(s) 319, 787, 835
HpyCH4V TGCA 5 cut(s) 224, 347, 361, 512, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 344, 515, 668
HpyF3I CTNAG 4 cut(s) 333, 572, 593, 938
HpySE526I ACGT 3 cut(s) 319, 787, 835
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 3 cut(s) 133, 697, 1008
HspAI GCGC 2 cut(s) 127, 338
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 382, 568
LmnI GCTCC 4 cut(s) 59, 122, 138, 947
Lsp1109I GCAGC 6 cut(s) 28, 316, 496, 502, 524, 695
LweI GCATC 5 cut(s) 334, 468, 695, 732, 943
MaeI CTAG 4 cut(s) 519, 732, 753, 897
MaeII ACGT 3 cut(s) 319, 787, 835
MalI GATC 2 cut(s) 384, 570
MboI GATC 2 cut(s) 382, 568
MboII GAAGA 7 cut(s) 91, 268, 288, 303, 392, 548, 590
MflI RGATCY 2 cut(s) 382, 568
MhlI GDGCHC 2 cut(s) 793, 944
MluCI AATT 7 cut(s) 263, 352, 371, 499, 504, 766, 927
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 969
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 3 cut(s) 492, 503, 891
MspA1I CMGCKG 2 cut(s) 329, 515
MspI CCGG 3 cut(s) 45, 58, 253
MspR9I CCNGG 1 cut(s) 58
MwoI GCNNNNNNNGC 4 cut(s) 335, 344, 515, 668
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 1 cut(s) 58
NdeII GATC 2 cut(s) 382, 568
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 3 cut(s) 133, 697, 1008
NlaIV GGNNCC 1 cut(s) 904
NmeAIII GCCGAG 1 cut(s) 196
NspI RCATGY 2 cut(s) 133, 697
PaeI GCATGC 1 cut(s) 133
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 5 cut(s) 106, 112, 142, 449, 801
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 8 cut(s) 14, 43, 331, 511, 514, 517, 685, 999
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Ppu21I YACGTR 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 944
PspN4I GGNNCC 1 cut(s) 904
PspPI GGNCC 1 cut(s) 806
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 382, 568
PvuII CAGCTG 2 cut(s) 329, 515
RsaI GTAC 3 cut(s) 322, 649, 786
RsaNI GTAC 3 cut(s) 321, 648, 785
SacI GAGCTC 1 cut(s) 944
SaqAI TTAA 3 cut(s) 492, 503, 891
SatI GCNGC 8 cut(s) 13, 42, 330, 510, 513, 516, 684, 998
Sau3AI GATC 2 cut(s) 382, 568
Sau96I GGNCC 1 cut(s) 806
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 58
SduI GDGCHC 2 cut(s) 793, 944
SfaNI GCATC 5 cut(s) 334, 468, 695, 732, 943
SfcI CTRYAG 2 cut(s) 324, 510
SinI GGWCC 1 cut(s) 806
SmlI CTYRAG 5 cut(s) 101, 191, 604, 775, 907
SmoI CTYRAG 5 cut(s) 101, 191, 604, 775, 907
SphI GCATGC 1 cut(s) 133
Sse9I AATT 7 cut(s) 263, 352, 371, 499, 504, 766, 927
SsiI CCGC 4 cut(s) 12, 25, 93, 149
SspI AATATT 1 cut(s) 240
SspMI CTAG 4 cut(s) 519, 732, 753, 897
SstI GAGCTC 1 cut(s) 944
StyD4I CCNGG 1 cut(s) 56
StyI CCWWGG 2 cut(s) 752, 896
TaiI ACGT 3 cut(s) 322, 790, 838
TaqI TCGA 2 cut(s) 75, 123
TaqII GACCGA 2 cut(s) 428, 794
TasI AATT 7 cut(s) 263, 352, 371, 499, 504, 766, 927
TatI WGTACW 1 cut(s) 647
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 5 cut(s) 106, 112, 142, 449, 801
Tru1I TTAA 3 cut(s) 492, 503, 891
Tru9I TTAA 3 cut(s) 492, 503, 891
TscAI CASTG 3 cut(s) 580, 673, 706
TseI GCWGC 7 cut(s) 41, 329, 509, 512, 515, 683, 997
TspDTI ATGAA 5 cut(s) 200, 627, 645, 801, 816
TspGWI ACGGA 3 cut(s) 33, 109, 125
TspRI CASTG 3 cut(s) 580, 673, 706
VpaK11BI GGWCC 1 cut(s) 806
XapI RAATTY 2 cut(s) 263, 352
XceI RCATGY 2 cut(s) 133, 697
XmaJI CCTAGG 2 cut(s) 752, 896
XmiI GTMKAC 1 cut(s) 848
XspI CTAG 4 cut(s) 519, 732, 753, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.