Rh5DG310900

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008484
Physical Location & Seq
Reverse (-)
39667992 .. 39675769
7778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG310900.1

Sequence Viewer

Length: 750 bp
ATGGCTCACGGCGGCTACGGAAAGCGGAGGGTGAAGCCTGCTGCCGGCCGTCGCTCCCGGACGGGCTTGGGCGTCGAGAAGAAGCCCAAGTCCGCCTCCCTCAAGAATCAGATTCGCTCCGTCGAGCGCATGCTCCGTAAGAATCTACCGCCTGAAGTTAGGGAGGTTCAGGAAAAGAAGTTAGAAGGACTCAAGAAACAGCAAGATATTCATACTCGGCTTGCAGTAGAACGCAAAATATTTTTGCGAGACCGGAAGATAAAATTTTTTGAGAGAAGAAAGATAGAGAGAAGAATAAGACGCTTGGAGAAACTTCAACGTACTACAGCTGCTCAGGCGCAAGATGCAGAGAATTCTGTGCAACTTTCCAAATTGAAAGAAGATCTTGAATATGTTAGGTTCTTTCCCAAGACCGAGAAATATGTCGCTCTGTTTACTGGAGGTGATGATTCAGAGCTTGTTGATAGGAGAAATAGGATGCGTAAGCAGATTAAGGCCAATTTAATTGCTGCAGCTGCTAGTGGGAAGGATTTGGAAGAAACAGGGAGTGAGGATGATGGGCTTTTGGATCTCAGTGAAGATGATTTCTTTCTAAGTGGAAGCTCAAGTGATGAAGCAAATGCAGATGATGAATGGACAGATAAGAGTACAAGGGAACAGGCTTCCAGTGCTTCTGGGAAAGCAGCATCTGGCATGTCCAGTGATGAGAGAAATCAGGTTTTCTTTCTACTGATTATGGCACTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Efg1

Protein Analysis

249

Amino Acids

28.35

Weight (kDa)

9.74

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Efg1 PF10153 34 - 145 5.8e-30 rRNA-processing protein Efg1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 12, 25, 93, 149
AclWI GGATC 1 cut(s) 576
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 263, 352
AcuI CTGAAG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 322, 649
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 3 cut(s) 317, 376, 389
AluBI AGCT 4 cut(s) 329, 457, 515, 603
AluI AGCT 4 cut(s) 329, 457, 515, 603
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 1 cut(s) 576
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 2 cut(s) 46, 495
ApeKI GCWGC 6 cut(s) 41, 329, 509, 512, 515, 683
ApoI RAATTY 2 cut(s) 263, 352
AspLEI GCGC 2 cut(s) 129, 340
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 43, 455
BbvI GCAGC 6 cut(s) 28, 316, 496, 502, 524, 695
BccI CCATC 1 cut(s) 551
BceAI ACGGC 2 cut(s) 25, 33
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 1 cut(s) 519
BfmI CTRYAG 2 cut(s) 324, 510
BglII AGATCT 1 cut(s) 382
BisI GCNGC 7 cut(s) 13, 42, 330, 510, 513, 516, 684
BlsI GCNGC 7 cut(s) 14, 43, 331, 511, 514, 517, 685
Bme1390I CCNGG 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 58
BmsI GCATC 3 cut(s) 334, 468, 695
BpmI CTGGAG 1 cut(s) 459
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 3 cut(s) 86, 176, 589
BpuMI CCSGG 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 243
BsaWI WCCGGW 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 3 cut(s) 442, 666, 699
BseGI GGATG 2 cut(s) 483, 559
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 2 cut(s) 347, 586
BseNI ACTGG 3 cut(s) 442, 666, 699
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 6 cut(s) 28, 316, 496, 502, 524, 695
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 2 cut(s) 48, 497
BsiEI CGRYCG 1 cut(s) 49
BsiSI CCGG 3 cut(s) 45, 58, 253
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 243
BsnI GGCC 2 cut(s) 48, 497
Bso31I GGTCTC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 382, 568
BspACI CCGC 4 cut(s) 12, 25, 93, 149
BspANI GGCC 2 cut(s) 48, 497
BspCNI CTCAG 2 cut(s) 346, 585
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 1 cut(s) 576
BspTNI GGTCTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 3 cut(s) 442, 666, 699
BssAI RCCGGY 1 cut(s) 44
BssMI GATC 2 cut(s) 382, 568
BssNI GRCGYC 1 cut(s) 72
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 4 cut(s) 39, 46, 131, 222
BstDEI CTNAG 3 cut(s) 333, 572, 593
BstF5I GGATG 2 cut(s) 483, 559
BstHHI GCGC 2 cut(s) 129, 340
BstKTI GATC 2 cut(s) 385, 571
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 2 cut(s) 382, 568
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 4 cut(s) 335, 344, 515, 668
BstNSI RCATGY 2 cut(s) 133, 697
BstSCI CCNGG 1 cut(s) 56
BstSFI CTRYAG 2 cut(s) 324, 510
BstV1I GCAGC 6 cut(s) 28, 316, 496, 502, 524, 695
BstX2I RGATCY 2 cut(s) 382, 568
BstYI RGATCY 2 cut(s) 382, 568
BstZI CGGCCG 1 cut(s) 46
BsuRI GGCC 2 cut(s) 48, 497
BtsCI GGATG 2 cut(s) 483, 559
BtsIMutI CAGTG 3 cut(s) 580, 673, 706
Cac8I GCNNGC 4 cut(s) 39, 46, 131, 222
CaiI CAGNNNCTG 1 cut(s) 689
CfoI GCGC 2 cut(s) 129, 340
Cfr10I RCCGGY 1 cut(s) 44
CseI GACGC 2 cut(s) 61, 309
Csp6I GTAC 2 cut(s) 321, 648
CviAII CATG 2 cut(s) 130, 694
CviQI GTAC 2 cut(s) 321, 648
DdeI CTNAG 3 cut(s) 333, 572, 593
DpnI GATC 2 cut(s) 384, 570
DpnII GATC 2 cut(s) 382, 568
EaeI YGGCCR 1 cut(s) 46
EagI CGGCCG 1 cut(s) 46
EciI GGCGGA 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 243
Eco52I CGGCCG 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 174
EcoRI GAATTC 1 cut(s) 352
FaeI CATG 2 cut(s) 133, 697
FaiI YATR 6 cut(s) 131, 213, 393, 423, 695, 737
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FatI CATG 2 cut(s) 129, 693
Fnu4HI GCNGC 7 cut(s) 13, 42, 330, 510, 513, 516, 684
FokI GGATG 2 cut(s) 490, 566
Fsp4HI GCNGC 7 cut(s) 13, 42, 330, 510, 513, 516, 684
FspBI CTAG 1 cut(s) 519
GlaI GCGC 2 cut(s) 128, 339
GluI GCNGC 7 cut(s) 13, 42, 330, 510, 513, 516, 684
GsuI CTGGAG 1 cut(s) 459
HaeIII GGCC 2 cut(s) 48, 497
HapII CCGG 3 cut(s) 45, 58, 253
HgaI GACGC 2 cut(s) 61, 309
HhaI GCGC 2 cut(s) 129, 340
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 2 cut(s) 133, 697
Hin6I GCGC 2 cut(s) 127, 338
HinP1I GCGC 2 cut(s) 127, 338
HinfI GANTC 5 cut(s) 106, 112, 142, 189, 449
HpaII CCGG 3 cut(s) 45, 58, 253
HphI GGTGA 2 cut(s) 43, 455
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 2 cut(s) 111, 454
Hpy188III TCNNGA 5 cut(s) 76, 103, 170, 193, 386
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 3 cut(s) 54, 77, 125
HpyAV CCTTC 2 cut(s) 179, 520
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 5 cut(s) 224, 347, 361, 512, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 344, 515, 668
HpyF3I CTNAG 3 cut(s) 333, 572, 593
HpySE526I ACGT 1 cut(s) 319
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 2 cut(s) 133, 697
HspAI GCGC 2 cut(s) 127, 338
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 382, 568
LmnI GCTCC 3 cut(s) 59, 122, 138
Lsp1109I GCAGC 6 cut(s) 28, 316, 496, 502, 524, 695
LweI GCATC 3 cut(s) 334, 468, 695
MaeI CTAG 1 cut(s) 519
MaeII ACGT 1 cut(s) 319
MalI GATC 2 cut(s) 384, 570
MboI GATC 2 cut(s) 382, 568
MboII GAAGA 7 cut(s) 91, 268, 288, 303, 392, 548, 590
MflI RGATCY 2 cut(s) 382, 568
MluCI AATT 5 cut(s) 263, 352, 371, 499, 504
MlyI GAGTC 1 cut(s) 183
MnlI CCTC 6 cut(s) 21, 106, 110, 157, 434, 544
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 2 cut(s) 492, 503
MspA1I CMGCKG 2 cut(s) 329, 515
MspI CCGG 3 cut(s) 45, 58, 253
MspR9I CCNGG 1 cut(s) 58
MwoI GCNNNNNNNGC 4 cut(s) 335, 344, 515, 668
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 1 cut(s) 58
NdeII GATC 2 cut(s) 382, 568
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 2 cut(s) 133, 697
NmeAIII GCCGAG 1 cut(s) 196
NspI RCATGY 2 cut(s) 133, 697
PaeI GCATGC 1 cut(s) 133
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 4 cut(s) 106, 112, 142, 449
PfoI TCCNGGA 1 cut(s) 56
PkrI GCNGC 7 cut(s) 14, 43, 331, 511, 514, 517, 685
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 382, 568
PvuII CAGCTG 2 cut(s) 329, 515
RsaI GTAC 2 cut(s) 322, 649
RsaNI GTAC 2 cut(s) 321, 648
SaqAI TTAA 2 cut(s) 492, 503
SatI GCNGC 7 cut(s) 13, 42, 330, 510, 513, 516, 684
Sau3AI GATC 2 cut(s) 382, 568
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 58
SetI ASST 9 cut(s) 168, 322, 331, 401, 445, 459, 517, 605, 720
SfaNI GCATC 3 cut(s) 334, 468, 695
SfcI CTRYAG 2 cut(s) 324, 510
SmlI CTYRAG 3 cut(s) 101, 191, 604
SmoI CTYRAG 3 cut(s) 101, 191, 604
SphI GCATGC 1 cut(s) 133
Sse9I AATT 5 cut(s) 263, 352, 371, 499, 504
SsiI CCGC 4 cut(s) 12, 25, 93, 149
SspI AATATT 1 cut(s) 240
SspMI CTAG 1 cut(s) 519
StyD4I CCNGG 1 cut(s) 56
TaiI ACGT 1 cut(s) 322
TaqI TCGA 2 cut(s) 75, 123
TaqII GACCGA 1 cut(s) 428
TasI AATT 5 cut(s) 263, 352, 371, 499, 504
TatI WGTACW 1 cut(s) 647
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 4 cut(s) 106, 112, 142, 449
Tru1I TTAA 2 cut(s) 492, 503
Tru9I TTAA 2 cut(s) 492, 503
TscAI CASTG 3 cut(s) 580, 673, 706
TseI GCWGC 6 cut(s) 41, 329, 509, 512, 515, 683
TspDTI ATGAA 3 cut(s) 200, 627, 645
TspGWI ACGGA 3 cut(s) 33, 109, 125
TspRI CASTG 3 cut(s) 580, 673, 706
XapI RAATTY 2 cut(s) 263, 352
XceI RCATGY 2 cut(s) 133, 697
XspI CTAG 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.