MD13G1204100.v1.1

rRNA-processing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008484
Physical Location & Seq
Forward (+)
18375894 .. 18380355
4462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1204100.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGCTCACGGTGGCTACAGCAAGCGGCGGGTGAACCCCGCCGGACGTCGGTCCAAGTCGGTCTTGGGCCCGGAGAAGAAGCCCAAGTCCGTCACCCTCAAAAACCAGATACGCTCCATCGAGCGCATGCTCCGTAAGAATTTACCAGCTGAAGTTAGGGAGGCTCAGGAAAAGAAGTTGGAAGGGTTCAAGAAACAGCAGGAGATTCATTCCCGTCTCGCTGTGGAACGCAAAATATTTATGCGTGACAGGAAGATAAAGTTTTTTGAGAGAAGAAAGATAGAGAGAAGATTAAGACGTCTGGAGAAACTTCAACGTGCTTCTTCTGGTCAGGCACAAGATGCAGAGATTTTGTTGCAACTTTCCAAATTGAAAGAAGATCTTGAATATGTTAGGTTCTTTCCCAAGACCGAAAAATATGTCTCGTTGTTTACTGGAGGTGAAGATACAGATGTGATTGATAAGAGAAATAAGTTGCGTAAGCAGATTAAGGCCAATATAGTTGCTGCAGCAGCTAGCGGAAAGGATCTGGAAGAAACAGGGAGTGAGGATGACGGGCTTCTGGATATGAGCGAAGATGATTTCTTCCTAAATGGGAGTTCTAGTGATGAAGCAGATGCTGACGATGAATGGACAGATAAGAGTGCAAAGGAACAGGCTTCCAGTGCTTCTGGAAAAGCAACTTCTGGCATGTCCAGTGATGAGAGAAATCAGATTTCTGCTAGAGCTTTGATGCCTCCACCCCGGCCATCCAGCAATTCTCACTCAAGTTCAGTTTGTGCTCAGTCAAGGTTTGGTCCTTCATCGAGCAAAAATTCCTCAAAAAAGCCTGCTGAAATGACCACATCAAGCAATACATCAAATAGCAGAAGTGGAACTTCCTTCAATACATCAAATAGCAATACATCAAGTAGCAGAAGTGAAACTTCCTTCAAAGATAGGGGATCCTCTAATTCAACTGCTGGTCAAAGTAGCAATTTGAGCTCCAACTCTGATGCTCATAAGCCCCGTAGAAAAAGGAGGCCAAAGAAGAAAAAGCAGCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Efg1

Protein Analysis

350

Amino Acids

38.9

Weight (kDa)

10.09

Isoelectric Point (pI)

64.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Efg1 PF10153 33 - 144 9e-31 rRNA-processing protein Efg1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 49, 301
AciI CCGC 4 cut(s) 25, 28, 39, 519
AclWI GGATC 3 cut(s) 534, 939, 952
AcoI YGGCCR 1 cut(s) 746
AcsI RAATTY 2 cut(s) 139, 814
AcuI CTGAAG 1 cut(s) 171
AcyI GRCGYC 2 cut(s) 46, 298
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 7 cut(s) 190, 314, 373, 386, 886, 934, 957
AluBI AGCT 4 cut(s) 149, 515, 728, 984
AluI AGCT 4 cut(s) 149, 515, 728, 984
Alw21I GWGCWC 2 cut(s) 784, 986
Alw26I GTCTC 2 cut(s) 221, 427
AlwI GGATC 3 cut(s) 534, 939, 952
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 4 cut(s) 67, 492, 746, 1022
ApaI GGGCCC 1 cut(s) 71
ApeKI GCWGC 4 cut(s) 506, 509, 512, 1039
ApoI RAATTY 2 cut(s) 139, 814
ArsI GACNNNNNNTTYG 2 cut(s) 405, 437
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 4 cut(s) 51, 67, 68, 797
AsuC2I CCSGG 2 cut(s) 71, 745
AsuHPI GGTGA 3 cut(s) 43, 85, 452
AsuNHI GCTAGC 1 cut(s) 515
AvaII GGWCC 2 cut(s) 51, 797
BaeGI GKGCMC 1 cut(s) 71
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BamHI GGATCC 1 cut(s) 944
BanII GRGCYC 2 cut(s) 71, 986
Bbv12I GWGCWC 2 cut(s) 784, 986
BbvI GCAGC 3 cut(s) 493, 521, 524
BccI CCATC 2 cut(s) 125, 757
BcnI CCSGG 2 cut(s) 71, 745
BcoDI GTCTC 2 cut(s) 221, 427
BfaI CTAG 3 cut(s) 516, 603, 723
BfmI CTRYAG 2 cut(s) 16, 507
BglII AGATCT 1 cut(s) 379
BisI GCNGC 5 cut(s) 26, 507, 510, 513, 1040
BlsI GCNGC 5 cut(s) 27, 508, 511, 514, 1041
Bme1390I CCNGG 2 cut(s) 71, 745
Bme18I GGWCC 2 cut(s) 51, 797
BmgT120I GGNCC 4 cut(s) 51, 67, 68, 797
BmiI GGNNCC 2 cut(s) 69, 946
BmrFI CCNGG 2 cut(s) 71, 745
BmsI GCATC 4 cut(s) 331, 607, 723, 985
BmtI GCTAGC 1 cut(s) 519
BoxI GACNNNNGTC 1 cut(s) 49
BpmI CTGGAG 2 cut(s) 323, 456
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 751
BpuMI CCSGG 2 cut(s) 71, 745
BsaHI GRCGYC 2 cut(s) 46, 298
BsaJI CCNNGG 1 cut(s) 743
BsaXI ACNNNNNCTCC 2 cut(s) 589, 619
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 3 cut(s) 439, 663, 696
BseDI CCNNGG 1 cut(s) 743
BseGI GGATG 2 cut(s) 556, 749
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMII CTCAG 2 cut(s) 179, 797
BseNI ACTGG 3 cut(s) 439, 663, 696
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 3 cut(s) 493, 521, 524
BshFI GGCC 4 cut(s) 69, 494, 748, 1024
BsiHKAI GWGCWC 2 cut(s) 784, 986
BsiSI CCGG 3 cut(s) 42, 71, 745
BslI CCNNNNNNNGG 1 cut(s) 48
BsmAI GTCTC 2 cut(s) 221, 427
BsmBI CGTCTC 1 cut(s) 221
BsnI GGCC 4 cut(s) 69, 494, 748, 1024
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 3 cut(s) 71, 784, 986
Bsp143I GATC 3 cut(s) 379, 526, 944
BspACI CCGC 4 cut(s) 25, 28, 39, 519
BspANI GGCC 4 cut(s) 69, 494, 748, 1024
BspCNI CTCAG 2 cut(s) 178, 796
BspLI GGNNCC 2 cut(s) 69, 946
BspMAI CTGCAG 1 cut(s) 511
BspOI GCTAGC 1 cut(s) 519
BspPI GGATC 3 cut(s) 534, 939, 952
BsrI ACTGG 3 cut(s) 439, 663, 696
BssECI CCNNGG 1 cut(s) 743
BssMI GATC 3 cut(s) 379, 526, 944
BssNI GRCGYC 2 cut(s) 46, 298
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 2 cut(s) 46, 298
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 4 cut(s) 23, 128, 517, 831
BstDEI CTNAG 2 cut(s) 165, 783
BstF5I GGATG 2 cut(s) 556, 749
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 3 cut(s) 382, 529, 947
BstMAI GTCTC 2 cut(s) 221, 427
BstMBI GATC 3 cut(s) 379, 526, 944
BstMWI GCNNNNNNNGC 4 cut(s) 341, 512, 665, 981
BstNSI RCATGY 2 cut(s) 130, 694
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 69, 743
BstSFI CTRYAG 2 cut(s) 16, 507
BstSLI GKGCMC 1 cut(s) 71
BstV1I GCAGC 3 cut(s) 493, 521, 524
BstX2I RGATCY 3 cut(s) 379, 526, 944
BstYI RGATCY 3 cut(s) 379, 526, 944
BsuRI GGCC 4 cut(s) 69, 494, 748, 1024
BtsCI GGATG 2 cut(s) 556, 749
BtsIMutI CAGTG 2 cut(s) 670, 703
Cac8I GCNNGC 4 cut(s) 23, 128, 517, 831
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 126
Cfr13I GGNCC 4 cut(s) 51, 67, 68, 797
CviAII CATG 2 cut(s) 127, 691
DdeI CTNAG 2 cut(s) 165, 783
DpnI GATC 3 cut(s) 381, 528, 946
DpnII GATC 3 cut(s) 379, 526, 944
EaeI YGGCCR 1 cut(s) 746
Ecl136II GAGCTC 1 cut(s) 984
Eco24I GRGCYC 2 cut(s) 71, 986
Eco47I GGWCC 2 cut(s) 51, 797
Eco53kI GAGCTC 1 cut(s) 984
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 984
EcoT38I GRGCYC 2 cut(s) 71, 986
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 2 cut(s) 130, 694
FaiI YATR 8 cut(s) 128, 242, 390, 420, 500, 569, 692, 1002
FalI AAGNNNNNCTT 8 cut(s) 47, 79, 366, 398, 862, 894, 910, 942
FatI CATG 2 cut(s) 126, 690
FauI CCCGC 2 cut(s) 21, 46
Fnu4HI GCNGC 5 cut(s) 26, 507, 510, 513, 1040
FokI GGATG 2 cut(s) 563, 736
FriOI GRGCYC 2 cut(s) 71, 986
Fsp4HI GCNGC 5 cut(s) 26, 507, 510, 513, 1040
FspBI CTAG 3 cut(s) 516, 603, 723
GlaI GCGC 1 cut(s) 125
GluI GCNGC 5 cut(s) 26, 507, 510, 513, 1040
GsuI CTGGAG 2 cut(s) 323, 456
HaeIII GGCC 4 cut(s) 69, 494, 748, 1024
HapII CCGG 3 cut(s) 42, 71, 745
HhaI GCGC 1 cut(s) 126
Hin1I GRCGYC 2 cut(s) 46, 298
Hin1II CATG 2 cut(s) 130, 694
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HinfI GANTC 1 cut(s) 205
HpaII CCGG 3 cut(s) 42, 71, 745
HphI GGTGA 3 cut(s) 43, 85, 452
Hpy166II GTNNAC 2 cut(s) 34, 432
Hpy188I TCNGA 2 cut(s) 714, 994
Hpy188III TCNNGA 7 cut(s) 167, 190, 302, 383, 530, 563, 672
Hpy8I GTNNAC 2 cut(s) 34, 432
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 4 cut(s) 176, 810, 892, 940
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 3 cut(s) 46, 298, 316
HpyCH4V TGCA 4 cut(s) 344, 358, 509, 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 512, 665, 981
HpyF3I CTNAG 2 cut(s) 165, 783
HpySE526I ACGT 3 cut(s) 46, 298, 316
Hsp92I GRCGYC 2 cut(s) 46, 298
Hsp92II CATG 2 cut(s) 130, 694
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 3 cut(s) 379, 526, 944
LmnI GCTCC 3 cut(s) 119, 135, 989
Lsp1109I GCAGC 3 cut(s) 493, 521, 524
LweI GCATC 4 cut(s) 331, 607, 723, 985
MaeI CTAG 3 cut(s) 516, 603, 723
MaeII ACGT 3 cut(s) 46, 298, 316
MaeIII GTNAC 2 cut(s) 91, 245
MalI GATC 3 cut(s) 381, 528, 946
MboI GATC 3 cut(s) 379, 526, 944
MflI RGATCY 3 cut(s) 379, 526, 944
MhlI GDGCHC 3 cut(s) 71, 784, 986
MluCI AATT 6 cut(s) 139, 368, 757, 814, 952, 976
MmeI TCCRAC 2 cut(s) 159, 1011
MnlI CCTC 8 cut(s) 107, 154, 431, 541, 747, 829, 958, 1014
MseI TTAA 2 cut(s) 293, 489
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 3 cut(s) 42, 71, 745
MspR9I CCNGG 2 cut(s) 71, 745
MwoI GCNNNNNNNGC 4 cut(s) 341, 512, 665, 981
NciI CCSGG 2 cut(s) 71, 745
NdeII GATC 3 cut(s) 379, 526, 944
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 2 cut(s) 130, 694
NlaIV GGNNCC 2 cut(s) 69, 946
NmuCI GTSAC 2 cut(s) 91, 245
NspI RCATGY 2 cut(s) 130, 694
PaeI GCATGC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 205
PkrI GCNGC 5 cut(s) 27, 508, 511, 514, 1041
PshAI GACNNNNGTC 1 cut(s) 49
Psp124BI GAGCTC 1 cut(s) 986
PspN4I GGNNCC 2 cut(s) 69, 946
PspOMI GGGCCC 1 cut(s) 67
PspPI GGNCC 4 cut(s) 51, 67, 68, 797
PstI CTGCAG 1 cut(s) 511
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 3 cut(s) 379, 526, 944
PvuII CAGCTG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 986
SaqAI TTAA 2 cut(s) 293, 489
SatI GCNGC 5 cut(s) 26, 507, 510, 513, 1040
Sau3AI GATC 3 cut(s) 379, 526, 944
Sau96I GGNCC 4 cut(s) 51, 67, 68, 797
ScrFI CCNGG 2 cut(s) 71, 745
SduI GDGCHC 3 cut(s) 71, 784, 986
SfaNI GCATC 4 cut(s) 331, 607, 723, 985
SfcI CTRYAG 2 cut(s) 16, 507
SinI GGWCC 2 cut(s) 51, 797
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
SphI GCATGC 1 cut(s) 130
Sse9I AATT 6 cut(s) 139, 368, 757, 814, 952, 976
SsiI CCGC 4 cut(s) 25, 28, 39, 519
SspI AATATT 1 cut(s) 237
SspMI CTAG 3 cut(s) 516, 603, 723
SstI GAGCTC 1 cut(s) 986
StyD4I CCNGG 2 cut(s) 69, 743
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 3 cut(s) 49, 301, 319
TaqI TCGA 2 cut(s) 120, 806
TaqII GACCGA 3 cut(s) 39, 49, 425
TasI AATT 6 cut(s) 139, 368, 757, 814, 952, 976
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 1 cut(s) 205
Tru1I TTAA 2 cut(s) 293, 489
Tru9I TTAA 2 cut(s) 293, 489
TscAI CASTG 2 cut(s) 670, 703
TseFI GTSAC 2 cut(s) 91, 245
TseI GCWGC 4 cut(s) 506, 509, 512, 1039
Tsp45I GTSAC 2 cut(s) 91, 245
TspDTI ATGAA 4 cut(s) 197, 624, 642, 792
TspGWI ACGGA 2 cut(s) 79, 122
TspRI CASTG 2 cut(s) 670, 703
VpaK11BI GGWCC 2 cut(s) 51, 797
XapI RAATTY 2 cut(s) 139, 814
XceI RCATGY 2 cut(s) 130, 694
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XspI CTAG 3 cut(s) 516, 603, 723
ZraI GACGTC 2 cut(s) 47, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.