AT1G06190

Rho termination factor, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007951
Physical Location & Seq
Reverse (-)
1892231 .. 1894194
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G06190.3

Sequence Viewer

Length: 1206 bp
ATGGCGATGTCGGGAACTTTCCATTTGACTTCTGACTATGTCCCTGGTTACACACTTTCAGACAGCCGTTGCTTCTTTAACTCTGCTGTTTCAAGAAGAACACTTGCCATATTACCTTGCTCTTCCTGTCTCGATCACAAGAATGGGCGTCTGAAATCTGTCCCCAATAGAAGTTCTTTTGTGTGCCGAGCAAGTTCTGGCGGCTATAGGAGAAATCCTGATTTCTCAAGACTTAATAAGCATGGTTACCGGGGAAACAACAGGCAAAGCGGAGGGAGAGAAGATTTTGATATCGAAAACTCTGATATGTTGTCTTCAAGAAATGGGCCTTTATTCAACCTGTCTAGTTCCCCCAAATTCCAAGCTACCTCATCCCCTGGACCTAGAGAGAAAGAGATCGTGGAGCTTTTCAGAAAGGTTCAAGCTCAGCTCCGAGCTCGTGCTGCAGCAAAGAAAGAAGAAAAAAAGATTGAAGAAGCTTCTAAAGGGCAGGGAAAAGAAAGTGAAACTGTTGACTCTCTTCTTAAGTTACTAAGGAAGCACTCGGGAGAGCAAAGCAAGAGGCAAGTTAGCAAATTTAGCAGCCAGGGAGAAGTACAAGGAGATACTGTTGACAAACAAGATCGCACCGGAAATCTTGTCACTTCAGGGAACAAAGATAATAATGCATCATCCTTTACCAGACCAACATCAAGCTTCAGAAGAAAGTCGCCAGTACCAAGATCTCAATCACCGCCAGCTTATTCTAGCGAGGCAACTTTTGATCAATCATCAAGTTACAGTGTAACCTGGACCCAGAAGAAGGATACAGTAGAGTTGCATGATGAGCCTGAACATGAGCCTGCGTATGAGCATGAGCATGAGCCTGAAAATGAGTCAGAACCTGGGCCTGTGACAACTATGCTTGAACCAGATTCAGAGCTGAAACCAGAGTCATCATCATTTTATCAAGAGGAGGAGGATGATGATGTTACTTTTGATGTGTTATCACAGGATGATGGTATTTTAGATGTGTTATCAGATGATGATGAGTCTCTCGATGATGCTGATGAAGATAGTGATGAAGCTGAGGAAGAAGCCGTGAAAGACTTGAGTGAATTGAAGCTGGTCGAACTTAGAGGCATAGCAAAGTCACGGGGACTAAAAGGGTTGTCAAAGATGAAGAAAGCCGAATTAGTGGAGTTGCTTGGTAGTGATTCCAGCTGA

Protein Analysis

401

Amino Acids

44.53

Weight (kDa)

5.06

Isoelectric Point (pI)

62.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 261 - 292 2.6e-06 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 201, 270, 734
AcsI RAATTY 2 cut(s) 356, 575
AcuI CTGAAG 2 cut(s) 630, 682
AcyI GRCGYC 1 cut(s) 148
AfaI GTAC 2 cut(s) 597, 717
AfiI CCNNNNNNNGG 1 cut(s) 802
AflII CTTAAG 1 cut(s) 524
AgsI TTSAA 7 cut(s) 93, 318, 337, 422, 473, 908, 1102
AjnI CCWGG 5 cut(s) 43, 376, 585, 788, 883
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 2 cut(s) 134, 1038
AlwNI CAGNNNCTG 1 cut(s) 884
Ama87I CYCGRG 1 cut(s) 544
AoxI GGCC 2 cut(s) 326, 887
ApeKI GCWGC 3 cut(s) 443, 446, 582
ApoI RAATTY 2 cut(s) 356, 575
AspS9I GGNCC 4 cut(s) 326, 380, 792, 887
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 1 cut(s) 723
AvaI CYCGRG 1 cut(s) 544
AvaII GGWCC 2 cut(s) 380, 792
BanII GRGCYC 1 cut(s) 439
BauI CACGAG 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 439
BbvCI CCTCAGC 1 cut(s) 1068
BbvI GCAGC 3 cut(s) 430, 458, 594
BccI CCATC 1 cut(s) 992
BceAI ACGGC 2 cut(s) 51, 1064
BciT130I CCWGG 5 cut(s) 45, 378, 587, 790, 885
BciVI GTATCC 1 cut(s) 799
BclI TGATCA 1 cut(s) 763
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 2 cut(s) 134, 1038
BfaI CTAG 3 cut(s) 345, 384, 747
BfmI CTRYAG 2 cut(s) 205, 444
BfrI CTTAAG 1 cut(s) 524
BfuI GTATCC 1 cut(s) 799
BglII AGATCT 1 cut(s) 722
BisI GCNGC 4 cut(s) 202, 444, 447, 583
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 4 cut(s) 203, 445, 448, 584
Bme1390I CCNGG 6 cut(s) 45, 251, 378, 587, 790, 885
Bme18I GGWCC 2 cut(s) 380, 792
BmeT110I CYCGRG 1 cut(s) 544
BmgT120I GGNCC 4 cut(s) 326, 380, 792, 887
BmiI GGNNCC 1 cut(s) 794
BmrFI CCNGG 6 cut(s) 45, 251, 378, 587, 790, 885
BmsI GCATC 2 cut(s) 677, 1033
BpiI GAAGAC 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 1068
Bpu1102I GCTNAGC 1 cut(s) 426
BpuEI CTTGAG 2 cut(s) 211, 1111
BpuMI CCSGG 1 cut(s) 251
BsaBI GATNNNNATC 1 cut(s) 727
BsaHI GRCGYC 1 cut(s) 148
BsaJI CCNNGG 5 cut(s) 43, 250, 376, 586, 884
BsaWI WCCGGW 1 cut(s) 629
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 1 cut(s) 802
Bse1I ACTGG 1 cut(s) 713
Bse8I GATNNNNATC 1 cut(s) 727
BseBI CCWGG 5 cut(s) 45, 378, 587, 790, 885
BseDI CCNNGG 5 cut(s) 43, 250, 376, 586, 884
BseGI GGATG 4 cut(s) 371, 671, 967, 1000
BseJI GATNNNNATC 1 cut(s) 727
BseLI CCNNNNNNNGG 1 cut(s) 802
BseMII CTCAG 2 cut(s) 440, 1059
BseNI ACTGG 1 cut(s) 713
BseRI GAGGAG 2 cut(s) 968, 971
BseXI GCAGC 3 cut(s) 430, 458, 594
BshFI GGCC 2 cut(s) 328, 889
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 544
BsiSI CCGG 2 cut(s) 250, 630
BslFI GGGAC 3 cut(s) 26, 146, 1152
BslI CCNNNNNNNGG 1 cut(s) 802
BsmAI GTCTC 2 cut(s) 134, 1038
BsmFI GGGAC 3 cut(s) 26, 146, 1152
BsnI GGCC 2 cut(s) 328, 889
BsoBI CYCGRG 1 cut(s) 544
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 5 cut(s) 133, 396, 622, 722, 763
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 3 cut(s) 201, 270, 734
BspANI GGCC 2 cut(s) 328, 889
BspCNI CTCAG 2 cut(s) 439, 1060
BspLI GGNNCC 1 cut(s) 794
BspMAI CTGCAG 1 cut(s) 448
BspQI GCTCTTC 1 cut(s) 127
BspTI CTTAAG 1 cut(s) 524
BsrI ACTGG 1 cut(s) 713
BssECI CCNNGG 5 cut(s) 43, 250, 376, 586, 884
BssMI GATC 5 cut(s) 133, 396, 622, 722, 763
BssNI GRCGYC 1 cut(s) 148
BssSI CACGAG 1 cut(s) 438
Bst2BI CACGAG 1 cut(s) 438
Bst2UI CCWGG 5 cut(s) 45, 378, 587, 790, 885
Bst4CI ACNGT 4 cut(s) 511, 610, 782, 811
Bst6I CTCTTC 2 cut(s) 127, 525
BstACI GRCGYC 1 cut(s) 148
BstAFI CTTAAG 1 cut(s) 524
BstC8I GCNNGC 2 cut(s) 738, 843
BstDEI CTNAG 4 cut(s) 426, 533, 1068, 1115
BstEII GGTNACC 1 cut(s) 245
BstF5I GGATG 4 cut(s) 371, 671, 967, 1000
BstKTI GATC 5 cut(s) 136, 399, 625, 725, 766
BstMAI GTCTC 2 cut(s) 134, 1038
BstMBI GATC 5 cut(s) 133, 396, 622, 722, 763
BstMWI GCNNNNNNNGC 3 cut(s) 443, 579, 826
BstNI CCWGG 5 cut(s) 45, 378, 587, 790, 885
BstPI GGTNACC 1 cut(s) 245
BstSCI CCNGG 6 cut(s) 43, 249, 376, 585, 788, 883
BstSFI CTRYAG 2 cut(s) 205, 444
BstV1I GCAGC 3 cut(s) 430, 458, 594
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 722
BstYI RGATCY 1 cut(s) 722
BsuI GTATCC 1 cut(s) 799
BsuRI GGCC 2 cut(s) 328, 889
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 4 cut(s) 371, 671, 967, 1000
BtsIMutI CAGTG 1 cut(s) 787
Cac8I GCNNGC 2 cut(s) 738, 843
CaiI CAGNNNCTG 1 cut(s) 884
Cfr13I GGNCC 4 cut(s) 326, 380, 792, 887
CseI GACGC 1 cut(s) 137
Csp6I GTAC 2 cut(s) 596, 716
CviAII CATG 5 cut(s) 242, 821, 836, 854, 860
CviQI GTAC 2 cut(s) 596, 716
DdeI CTNAG 4 cut(s) 426, 533, 1068, 1115
DpnI GATC 5 cut(s) 135, 398, 624, 724, 765
DpnII GATC 5 cut(s) 133, 396, 622, 722, 763
Eam1104I CTCTTC 2 cut(s) 127, 525
EarI CTCTTC 2 cut(s) 127, 525
Ecl136II GAGCTC 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 439
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 380, 792
Eco53kI GAGCTC 1 cut(s) 437
Eco57I CTGAAG 2 cut(s) 630, 682
Eco88I CYCGRG 1 cut(s) 544
Eco91I GGTNACC 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 437
EcoO65I GGTNACC 1 cut(s) 245
EcoRII CCWGG 5 cut(s) 43, 376, 585, 788, 883
EcoRV GATATC 1 cut(s) 292
EcoT22I ATGCAT 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 439
FaeI CATG 5 cut(s) 245, 824, 839, 857, 863
FaqI GGGAC 3 cut(s) 26, 146, 1152
FatI CATG 5 cut(s) 241, 820, 835, 853, 859
FbaI TGATCA 1 cut(s) 763
Fnu4HI GCNGC 4 cut(s) 202, 444, 447, 583
FokI GGATG 4 cut(s) 358, 658, 974, 1007
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 4 cut(s) 202, 444, 447, 583
FspBI CTAG 3 cut(s) 345, 384, 747
GluI GCNGC 4 cut(s) 202, 444, 447, 583
HaeIII GGCC 2 cut(s) 328, 889
HapII CCGG 2 cut(s) 250, 630
HgaI GACGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 148
Hin1II CATG 5 cut(s) 245, 824, 839, 857, 863
HincII GTYRAC 2 cut(s) 514, 613
HindII GTYRAC 2 cut(s) 514, 613
HindIII AAGCTT 2 cut(s) 477, 694
HinfI GANTC 6 cut(s) 515, 875, 914, 932, 1031, 1196
HpaII CCGG 2 cut(s) 250, 630
HphI GGTGA 1 cut(s) 723
Hpy166II GTNNAC 2 cut(s) 514, 613
Hpy188III TCNNGA 9 cut(s) 12, 93, 131, 218, 228, 318, 546, 950, 1037
Hpy8I GTNNAC 2 cut(s) 514, 613
HpyAV CCTTC 1 cut(s) 796
HpyCH4III ACNGT 4 cut(s) 511, 610, 782, 811
HpyCH4V TGCA 3 cut(s) 446, 668, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 579, 826
HpyF3I CTNAG 4 cut(s) 426, 533, 1068, 1115
Hsp92I GRCGYC 1 cut(s) 148
Hsp92II CATG 5 cut(s) 245, 824, 839, 857, 863
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 5 cut(s) 133, 396, 622, 722, 763
LguI GCTCTTC 1 cut(s) 127
LmnI GCTCC 2 cut(s) 403, 435
Lsp1109I GCAGC 3 cut(s) 430, 458, 594
LweI GCATC 2 cut(s) 677, 1033
MaeI CTAG 3 cut(s) 345, 384, 747
MaeIII GTNAC 9 cut(s) 47, 245, 528, 640, 776, 784, 892, 970, 1131
MalI GATC 5 cut(s) 135, 398, 624, 724, 765
MboI GATC 5 cut(s) 133, 396, 622, 722, 763
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 4 cut(s) 356, 575, 1097, 1172
MlyI GAGTC 4 cut(s) 509, 884, 941, 1040
MnlI CCTC 9 cut(s) 266, 379, 555, 745, 946, 949, 952, 1063, 1112
Mph1103I ATGCAT 1 cut(s) 670
MseI TTAA 3 cut(s) 78, 234, 525
MslI CAYNNNNRTG 2 cut(s) 141, 858
MspA1I CMGCKG 1 cut(s) 1203
MspCI CTTAAG 1 cut(s) 524
MspI CCGG 2 cut(s) 250, 630
MspR9I CCNGG 6 cut(s) 45, 251, 378, 587, 790, 885
MvaI CCWGG 5 cut(s) 45, 378, 587, 790, 885
MwoI GCNNNNNNNGC 3 cut(s) 443, 579, 826
NciI CCSGG 1 cut(s) 251
NdeII GATC 5 cut(s) 133, 396, 622, 722, 763
NlaIII CATG 5 cut(s) 245, 824, 839, 857, 863
NlaIV GGNNCC 1 cut(s) 794
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 3 cut(s) 640, 892, 1131
NsiI ATGCAT 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 127
PfeI GAWTC 2 cut(s) 914, 1196
PflFI GACNNNGTC 1 cut(s) 38
PkrI GCNGC 4 cut(s) 203, 445, 448, 584
PleI GAGTC 4 cut(s) 509, 883, 940, 1039
PpsI GAGTC 4 cut(s) 509, 883, 940, 1039
Psp124BI GAGCTC 1 cut(s) 439
Psp6I CCWGG 5 cut(s) 43, 376, 585, 788, 883
PspEI GGTNACC 1 cut(s) 245
PspGI CCWGG 5 cut(s) 43, 376, 585, 788, 883
PspN4I GGNNCC 1 cut(s) 794
PspPI GGNCC 4 cut(s) 326, 380, 792, 887
PstI CTGCAG 1 cut(s) 448
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 1 cut(s) 722
PsyI GACNNNGTC 1 cut(s) 38
PvuII CAGCTG 1 cut(s) 1203
RsaI GTAC 2 cut(s) 597, 717
RsaNI GTAC 2 cut(s) 596, 716
RseI CAYNNNNRTG 2 cut(s) 141, 858
SacI GAGCTC 1 cut(s) 439
SapI GCTCTTC 1 cut(s) 127
SaqAI TTAA 3 cut(s) 78, 234, 525
SatI GCNGC 4 cut(s) 202, 444, 447, 583
Sau3AI GATC 5 cut(s) 133, 396, 622, 722, 763
Sau96I GGNCC 4 cut(s) 326, 380, 792, 887
SchI GAGTC 4 cut(s) 509, 884, 941, 1040
ScrFI CCNGG 6 cut(s) 45, 251, 378, 587, 790, 885
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 2 cut(s) 677, 1033
SfcI CTRYAG 2 cut(s) 205, 444
SinI GGWCC 2 cut(s) 380, 792
SmiMI CAYNNNNRTG 2 cut(s) 141, 858
SmlI CTYRAG 3 cut(s) 226, 524, 1090
SmoI CTYRAG 3 cut(s) 226, 524, 1090
Sse9I AATT 4 cut(s) 356, 575, 1097, 1172
SsiI CCGC 3 cut(s) 201, 270, 734
SspMI CTAG 3 cut(s) 345, 384, 747
SstI GAGCTC 1 cut(s) 439
StyD4I CCNGG 6 cut(s) 43, 249, 376, 585, 788, 883
TaaI ACNGT 4 cut(s) 511, 610, 782, 811
TaqI TCGA 4 cut(s) 132, 294, 1038, 1110
TasI AATT 4 cut(s) 356, 575, 1097, 1172
TatI WGTACW 1 cut(s) 595
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 2 cut(s) 914, 1196
Tru1I TTAA 3 cut(s) 78, 234, 525
Tru9I TTAA 3 cut(s) 78, 234, 525
TscAI CASTG 1 cut(s) 787
TseFI GTSAC 3 cut(s) 640, 892, 1131
TseI GCWGC 3 cut(s) 443, 446, 582
Tsp45I GTSAC 3 cut(s) 640, 892, 1131
TspDTI ATGAA 3 cut(s) 1065, 1077, 1175
TspRI CASTG 1 cut(s) 787
Tth111I GACNNNGTC 1 cut(s) 38
Vha464I CTTAAG 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 380, 792
XapI RAATTY 2 cut(s) 356, 575
XspI CTAG 3 cut(s) 345, 384, 747
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.