Rw6G026180

rho-N domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007951
Physical Location & Seq
Reverse (-)
49574755 .. 49576951
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026180.1

Sequence Viewer

Length: 1230 bp
ATGTCACAGAGCGTTCATCTCATCTGTTCTCGACCATCAGATAGCAAATGTCTACCTTGTTCTGGGATTTCGGGGAGAGTAGCCCCTGTATATCCTTGCTCTTCTCGTTTCAACCATAGATTCAAGTCGCAGGTCAGGGTTGGATCACTAAGAGGTGCTTATACTGGGGTGTCTTTTACGTGCAGAGCAGGTTCTAGTGGTCGTAATAGAAATCCAGACTTCCCAAGGCAAAACAGGCAAGGGTACTCTCGGGGGAGGAACAGGAGAAATGAAGAGAGAGACAGCTTTGAAAACCTTGAAGAATCTGATTTGCTTTCTGCTAAAAATGGTCCATTGCTCTCCCTCCCTAGTAACACAAAGTTTGGGGCTACTGCAGCCCCTGGTCCCAGAGAAAAAGAGATTGTTGAGCTGTTCAGGAAAGTTCAGGCTCAGCTCCGGGAGAGAACTGCTGTTAAAGAAGAAAAGAAGGTTGAACCCGTGCAAGGGCAAGGTAAAGATAATGGAACTGTGGATTCTCTTCTTAAACTACTAAGGAAACATTCGGCTGAACAGGCAAAGAGAAACAGTAATGGGGTTGGAAACAAAGACTTTATGTTGGACCAACCAGAAAAGACTGGCCGATATAGTGAAAGAAAAAACACGGCTTCTTTGGATTCAATTAACAGTGTGAAGGATTATGCTGAAGAGCCTACTGCCTCTTTGAGCAGGCCTGCATCAAATTTTCGACGCAAGTCGCCTATCAATCGATTGACATATCAACCTATTTATTCAGAGGATGATGAGATGGTCAATTCGGTGCAGCATGTGAATTCAACTGACAAAAGAAAAAAGAATTATGCTGAGAGAGTTTCTGAACCAGAACCTGAGCCAGAGCCAGAGCTTGAGATTGATGTGGAGTCGGTACTTGAGCCTGAGCCTGTTCATGATACTGAGTTTGGATTTGAACCTGAACCCGAGCCTGTTCATGATACCGAGTTTGGATTTGAACCTGAACCCGAGCCTGAGCAAGAGATTGTTCAGTTGTTCGAGGATGAGACTTCAGGTACTGAAGAAAATGTGGATGATGATGATGCGGACGAAGACAAAGAGCAACTTATTGAACAGACGGATTTGAGTGCATGTAAGCTGCCAGAATTGAGGGCACTTGCCAAGTCTCGAGGCGTGAAAGGGTTCTCAAAGATGAAGAAGGCCGAGCTTCTGGAGTTGCTAACTGGTGGGAGCTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

409

Amino Acids

45.88

Weight (kDa)

5.22

Isoelectric Point (pI)

54.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 370 - 401 1.7e-06 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 121, 179
AccI GTMKAC 1 cut(s) 52
AciI CCGC 1 cut(s) 1073
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 2 cut(s) 718, 808
AcuI CTGAAG 3 cut(s) 702, 1023, 1068
AfaI GTAC 3 cut(s) 245, 903, 1045
AfiI CCNNNNNNNGG 3 cut(s) 62, 482, 483
AjnI CCWGG 1 cut(s) 379
AluBI AGCT 7 cut(s) 285, 409, 433, 880, 1126, 1195, 1221
AluI AGCT 7 cut(s) 285, 409, 433, 880, 1126, 1195, 1221
Alw26I GTCTC 3 cut(s) 273, 1028, 1158
AlwI GGATC 1 cut(s) 151
AlwNI CAGNNNCTG 3 cut(s) 380, 863, 1046
Ama87I CYCGRG 4 cut(s) 249, 953, 995, 1155
AoxI GGCC 3 cut(s) 616, 707, 1188
ApeKI GCWGC 3 cut(s) 374, 799, 1126
ApoI RAATTY 2 cut(s) 718, 808
Asp700I GAANNNNTTC 1 cut(s) 1169
AspS9I GGNCC 3 cut(s) 329, 383, 598
AsuC2I CCSGG 1 cut(s) 437
AvaI CYCGRG 4 cut(s) 249, 953, 995, 1155
AvaII GGWCC 3 cut(s) 329, 383, 598
BaeGI GKGCMC 1 cut(s) 1144
BbsI GAAGAC 1 cut(s) 1086
BbvI GCAGC 3 cut(s) 386, 811, 1113
BccI CCATC 2 cut(s) 43, 778
BceAI ACGGC 1 cut(s) 657
BciT130I CCWGG 1 cut(s) 381
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 3 cut(s) 273, 1028, 1158
BfaI CTAG 2 cut(s) 195, 348
BfmI CTRYAG 1 cut(s) 372
BfuAI ACCTGC 2 cut(s) 121, 179
BisI GCNGC 3 cut(s) 375, 800, 1127
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 3 cut(s) 376, 801, 1128
Bme1390I CCNGG 2 cut(s) 381, 437
Bme18I GGWCC 3 cut(s) 329, 383, 598
BmeT110I CYCGRG 4 cut(s) 249, 953, 995, 1155
BmgT120I GGNCC 3 cut(s) 329, 383, 598
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 2 cut(s) 381, 437
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 722, 1060
BmuI ACTGGG 1 cut(s) 174
BoxI GACNNNNGTC 1 cut(s) 730
BpiI GAAGAC 1 cut(s) 1086
BpmI CTGGAG 1 cut(s) 1220
Bpu10I CCTNAGC 3 cut(s) 864, 912, 1002
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 2 cut(s) 902, 926
BpuMI CCSGG 1 cut(s) 437
Bsa29I ATCGAT 1 cut(s) 745
BsaAI YACGTR 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 224, 379
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 482, 483
Bse1I ACTGG 3 cut(s) 169, 619, 1216
Bse3DI GCAATG 1 cut(s) 332
BseBI CCWGG 1 cut(s) 381
BseCI ATCGAT 1 cut(s) 745
BseDI CCNNGG 2 cut(s) 224, 379
BseGI GGATG 3 cut(s) 781, 1036, 1066
BseLI CCNNNNNNNGG 3 cut(s) 62, 482, 483
BseMI GCAATG 1 cut(s) 332
BseMII CTCAG 6 cut(s) 443, 831, 855, 903, 921, 993
BseNI ACTGG 3 cut(s) 169, 619, 1216
BseSI GKGCMC 1 cut(s) 1144
BseXI GCAGC 3 cut(s) 386, 811, 1113
BsgI GTGCAG 2 cut(s) 202, 818
BshFI GGCC 3 cut(s) 618, 709, 1190
BshVI ATCGAT 1 cut(s) 745
BsiHKCI CYCGRG 4 cut(s) 249, 953, 995, 1155
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 62, 482, 483
BsmAI GTCTC 3 cut(s) 273, 1028, 1158
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 3 cut(s) 618, 709, 1190
BsoBI CYCGRG 4 cut(s) 249, 953, 995, 1155
Bsp1286I GDGCHC 1 cut(s) 1144
Bsp143I GATC 1 cut(s) 143
Bsp1720I GCTNAGC 1 cut(s) 429
BspACI CCGC 1 cut(s) 1073
BspANI GGCC 3 cut(s) 618, 709, 1190
BspCNI CTCAG 6 cut(s) 442, 832, 856, 904, 922, 994
BspDI ATCGAT 1 cut(s) 745
BspHI TCATGA 2 cut(s) 922, 964
BspLI GGNNCC 1 cut(s) 385
BspMAI CTGCAG 1 cut(s) 376
BspMI ACCTGC 2 cut(s) 121, 179
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 2 cut(s) 106, 678
BsrDI GCAATG 1 cut(s) 332
BsrI ACTGG 3 cut(s) 169, 619, 1216
BssECI CCNNGG 2 cut(s) 224, 379
BssMI GATC 1 cut(s) 143
BssT1I CCWWGG 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 3 cut(s) 508, 566, 665
Bst6I CTCTTC 4 cut(s) 106, 267, 522, 678
BstBAI YACGTR 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 707, 711
BstDEI CTNAG 9 cut(s) 149, 429, 530, 840, 864, 912, 930, 1002, 1222
BstF5I GGATG 3 cut(s) 781, 1036, 1066
BstKTI GATC 1 cut(s) 146
BstMAI GTCTC 3 cut(s) 273, 1028, 1158
BstMBI GATC 1 cut(s) 143
BstMWI GCNNNNNNNGC 3 cut(s) 235, 374, 551
BstNI CCWGG 1 cut(s) 381
BstNSI RCATGY 2 cut(s) 806, 1122
BstPAI GACNNNNGTC 1 cut(s) 730
BstSCI CCNGG 2 cut(s) 379, 435
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 1144
BstV1I GCAGC 3 cut(s) 386, 811, 1113
BstV2I GAAGAC 1 cut(s) 1086
Bsu15I ATCGAT 1 cut(s) 745
BsuRI GGCC 3 cut(s) 618, 709, 1190
BsuTUI ATCGAT 1 cut(s) 745
BtsCI GGATG 3 cut(s) 781, 1036, 1066
BtsIMutI CAGTG 1 cut(s) 670
BveI ACCTGC 2 cut(s) 121, 179
Cac8I GCNNGC 2 cut(s) 707, 711
CaiI CAGNNNCTG 3 cut(s) 380, 863, 1046
CciI TCATGA 2 cut(s) 922, 964
Cfr13I GGNCC 3 cut(s) 329, 383, 598
ClaI ATCGAT 1 cut(s) 745
CseI GACGC 1 cut(s) 735
Csp6I GTAC 3 cut(s) 244, 902, 1044
CviAII CATG 4 cut(s) 803, 923, 965, 1119
CviQI GTAC 3 cut(s) 244, 902, 1044
DdeI CTNAG 9 cut(s) 149, 429, 530, 840, 864, 912, 930, 1002, 1222
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 616
Eam1104I CTCTTC 4 cut(s) 106, 267, 522, 678
EarI CTCTTC 4 cut(s) 106, 267, 522, 678
Eco130I CCWWGG 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 709
Eco47I GGWCC 3 cut(s) 329, 383, 598
Eco57I CTGAAG 3 cut(s) 702, 1023, 1068
Eco88I CYCGRG 4 cut(s) 249, 953, 995, 1155
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 4 cut(s) 806, 926, 968, 1122
FalI AAGNNNNNCTT 4 cut(s) 142, 174, 1077, 1109
FaqI GGGAC 1 cut(s) 369
FatI CATG 4 cut(s) 802, 922, 964, 1118
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 3 cut(s) 375, 800, 1127
FokI GGATG 3 cut(s) 788, 1043, 1073
Fsp4HI GCNGC 3 cut(s) 375, 800, 1127
FspBI CTAG 2 cut(s) 195, 348
GluI GCNGC 3 cut(s) 375, 800, 1127
GsuI CTGGAG 1 cut(s) 1220
HaeIII GGCC 3 cut(s) 618, 709, 1190
HapII CCGG 1 cut(s) 436
HgaI GACGC 1 cut(s) 735
Hin1II CATG 4 cut(s) 806, 926, 968, 1122
HinfI GANTC 5 cut(s) 120, 302, 512, 653, 896
HpaII CCGG 1 cut(s) 436
Hpy166II GTNNAC 1 cut(s) 53
Hpy188I TCNGA 4 cut(s) 40, 307, 772, 853
Hpy188III TCNNGA 7 cut(s) 30, 215, 415, 923, 965, 1155, 1199
Hpy8I GTNNAC 1 cut(s) 53
Hpy99I CGWCG 1 cut(s) 729
HpyAV CCTTC 3 cut(s) 460, 664, 1180
HpyCH4III ACNGT 3 cut(s) 508, 566, 665
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 6 cut(s) 183, 374, 481, 713, 799, 1118
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 374, 551
HpyF3I CTNAG 9 cut(s) 149, 429, 530, 840, 864, 912, 930, 1002, 1222
HpySE526I ACGT 1 cut(s) 179
Hsp92II CATG 4 cut(s) 806, 926, 968, 1122
Kzo9I GATC 1 cut(s) 143
LguI GCTCTTC 2 cut(s) 106, 678
LmnI GCTCC 2 cut(s) 438, 1218
Lsp1109I GCAGC 3 cut(s) 386, 811, 1113
LweI GCATC 2 cut(s) 722, 1060
MaeI CTAG 2 cut(s) 195, 348
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 2 cut(s) 3, 350
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MboII GAAGA 9 cut(s) 93, 284, 311, 470, 509, 695, 1061, 1091, 1195
MhlI GDGCHC 1 cut(s) 1144
MluCI AATT 6 cut(s) 657, 718, 790, 808, 832, 1133
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 3 cut(s) 121, 556, 576
MnlI CCTC 8 cut(s) 146, 249, 353, 706, 766, 1021, 1131, 1151
MroXI GAANNNNTTC 1 cut(s) 1169
MseI TTAA 3 cut(s) 453, 522, 660
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 2 cut(s) 381, 437
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 3 cut(s) 235, 374, 551
NciI CCSGG 1 cut(s) 437
NdeII GATC 1 cut(s) 143
NlaIII CATG 4 cut(s) 806, 926, 968, 1122
NlaIV GGNNCC 1 cut(s) 385
NmeAIII GCCGAG 1 cut(s) 1216
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 2 cut(s) 806, 1122
PaeR7I CTCGAG 1 cut(s) 1155
PagI TCATGA 2 cut(s) 922, 964
PceI AGGCCT 1 cut(s) 709
PciSI GCTCTTC 2 cut(s) 106, 678
PdmI GAANNNNTTC 1 cut(s) 1169
PfeI GAWTC 4 cut(s) 120, 302, 512, 653
PfoI TCCNGGA 1 cut(s) 435
PkrI GCNGC 3 cut(s) 376, 801, 1128
PleI GAGTC 1 cut(s) 904
PpsI GAGTC 1 cut(s) 904
Ppu21I YACGTR 1 cut(s) 180
PshAI GACNNNNGTC 1 cut(s) 730
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 3 cut(s) 329, 383, 598
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 3 cut(s) 380, 863, 1046
RsaI GTAC 3 cut(s) 245, 903, 1045
RsaNI GTAC 3 cut(s) 244, 902, 1044
SapI GCTCTTC 2 cut(s) 106, 678
SaqAI TTAA 3 cut(s) 453, 522, 660
SatI GCNGC 3 cut(s) 375, 800, 1127
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 3 cut(s) 329, 383, 598
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 2 cut(s) 381, 437
SduI GDGCHC 1 cut(s) 1144
SfaNI GCATC 2 cut(s) 722, 1060
SfcI CTRYAG 1 cut(s) 372
Sfr274I CTCGAG 1 cut(s) 1155
SinI GGWCC 3 cut(s) 329, 383, 598
SlaI CTCGAG 1 cut(s) 1155
SmlI CTYRAG 3 cut(s) 881, 905, 1155
SmoI CTYRAG 3 cut(s) 881, 905, 1155
Sse9I AATT 6 cut(s) 657, 718, 790, 808, 832, 1133
SseBI AGGCCT 1 cut(s) 709
SsiI CCGC 1 cut(s) 1073
SspMI CTAG 2 cut(s) 195, 348
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 2 cut(s) 379, 435
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 3 cut(s) 508, 566, 665
TaiI ACGT 1 cut(s) 182
TaqI TCGA 5 cut(s) 31, 724, 745, 1026, 1156
TasI AATT 6 cut(s) 657, 718, 790, 808, 832, 1133
TfiI GAWTC 4 cut(s) 120, 302, 512, 653
Tru1I TTAA 3 cut(s) 453, 522, 660
Tru9I TTAA 3 cut(s) 453, 522, 660
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 374, 799, 1126
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 5 cut(s) 5, 285, 911, 953, 1196
TspGWI ACGGA 1 cut(s) 1121
TspRI CASTG 1 cut(s) 670
VpaK11BI GGWCC 3 cut(s) 329, 383, 598
XapI RAATTY 2 cut(s) 718, 808
XceI RCATGY 2 cut(s) 806, 1122
XhoI CTCGAG 1 cut(s) 1155
XmiI GTMKAC 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 1169
XspI CTAG 2 cut(s) 195, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.