MD05G1196200.v1.1

rho-N domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007951
Physical Location & Seq
Reverse (-)
32351942 .. 32354197
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1196200.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGTTACAAGGCGTTCATATCATCGGTTATGGACCATCAGATGCCAAATGTCTTCCTTATTCAGGAATTTCCGGAAGAGTAGCCCCTTTATGTCACTGCTCTTCTCGTTTCAACCATAAGTTCAAGTCACAGATCAGGATTGGATCACCGAGAGGTGCTTGCAAGGGGGTATCTTTTACGTGCAGAGCAAGTTCTAGTGGTCGTAATAGAAATCCAGACTTTCCAAGGCAAAACAGACATGGGTACTCCCGGAGCAGGAATAGGAAAAGTGTAGAAAGAGATGGCTTTGAAAACCTTGACGAATCTGATGTGCTTTCTTCTAAAAACGGGTCATTGGTCTCTCTTTCAAGTAACACAAAGTTCGGGGCCACTGCATCCCCTGGACCTAGAGAAAAAGAGATTGTTGAGCTGTTCCGGAAGGTCCAGACTCAACTTCGGGAGAGAAGTGCAGTTAAAGAAGAAAGGAAGGTTGAAGCTTCACAAGGGCATGGTAAAGAGAATGCGACTGTGGATTCCCTCCTTAACCTGTTAAGAAAACACTCGGTTGAGCAATCAAAGAGAAACAGCAACAGTGGCAGCAGCAACAAGGACTTCACGTTGGACCAACCAGAAAATAATTCCCGATATAGTGAAAGAAAAAACACAGCTTCTTTTGATTCAAATAACAAAGCTGCCTTTGATTCAAATAACCTTTTGAAGGACGAGGTTGAAGAACCTACAGCCTCCTTCAGCAGGCCTGCATCAAATTTTCAACGCAAGTCTCCCATTCCTCGGTTAACATACCAGGCCATTTATTCTGAGGATGATCACATAGGCAACACAGTACCACATGTGAATTCAACTGGGAAAAGAACGAAGAATCATGTTGACAGAGATCCTAAACCAGAGCCCGAGCCAAAGCTTGAGTTGGAGGCAGAACTTGACCATGAGCCCGAGCATGATGCTAAGTTTGAATTTGAGCCTGAACCTGAGCCTGAGCAAGGGATTTCTCAACTGTTAGAGGACCAGACCTCAGAAGATGAGATTTCAGTTCCTGAAGAAACTCTTATTGAAGGCAATGAGTACGAAGGAGAACTTGTTGAAAGTAGGGACTTGAGTACATTGAAGCTGCCAGAATTGAGGGTAATAGCAAAATCTCGAGGTGTAAAAGGGTTTTCGAAGATGAAGAAGGCTGAGCTTTTGGAGTTGCTAAGCAGAAGCGCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

403

Amino Acids

44.97

Weight (kDa)

6.63

Isoelectric Point (pI)

51.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 364 - 395 5.4e-07 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 71, 414
AclWI GGATC 2 cut(s) 151, 869
AcsI RAATTY 4 cut(s) 66, 745, 835, 953
AcuI CTGAAG 2 cut(s) 712, 1056
AfaI GTAC 4 cut(s) 245, 825, 1064, 1099
AfiI CCNNNNNNNGG 6 cut(s) 62, 255, 697, 732, 771, 980
AflIII ACRYGT 1 cut(s) 829
AjnI CCWGG 2 cut(s) 379, 783
AluBI AGCT 7 cut(s) 409, 476, 647, 671, 901, 1108, 1177
AluI AGCT 7 cut(s) 409, 476, 647, 671, 901, 1108, 1177
Alw26I GTCTC 2 cut(s) 343, 765
AlwI GGATC 2 cut(s) 151, 869
AlwNI CAGNNNCTG 1 cut(s) 1034
Ama87I CYCGRG 3 cut(s) 890, 932, 1137
Aor13HI TCCGGA 2 cut(s) 71, 414
AoxI GGCC 3 cut(s) 366, 734, 786
ApeKI GCWGC 4 cut(s) 576, 579, 671, 1108
ApoI RAATTY 4 cut(s) 66, 745, 835, 953
AspLEI GCGC 1 cut(s) 1202
AspS9I GGNCC 6 cut(s) 32, 366, 383, 421, 601, 1003
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 138
AsuII TTCGAA 1 cut(s) 1157
AvaI CYCGRG 3 cut(s) 890, 932, 1137
AvaII GGWCC 5 cut(s) 32, 383, 421, 601, 1003
BanII GRGCYC 2 cut(s) 891, 933
BbsI GAAGAC 1 cut(s) 44
BbvI GCAGC 4 cut(s) 588, 591, 658, 1095
BccI CCATC 2 cut(s) 43, 275
BcgI CGANNNNNNTGC 2 cut(s) 923, 957
BciT130I CCWGG 2 cut(s) 381, 785
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 343, 765
BfaI CTAG 2 cut(s) 195, 387
BfmI CTRYAG 1 cut(s) 717
BisI GCNGC 4 cut(s) 577, 580, 672, 1109
BlpI GCTNAGC 2 cut(s) 1173, 1190
BlsI GCNGC 4 cut(s) 578, 581, 673, 1110
Bme1390I CCNGG 3 cut(s) 250, 381, 785
Bme18I GGWCC 5 cut(s) 32, 383, 421, 601, 1003
BmeT110I CYCGRG 3 cut(s) 890, 932, 1137
BmgT120I GGNCC 6 cut(s) 32, 366, 383, 421, 601, 1003
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 3 cut(s) 250, 381, 785
BmrI ACTGGG 1 cut(s) 852
BmsI GCATC 4 cut(s) 31, 383, 749, 931
BmuI ACTGGG 1 cut(s) 852
BpiI GAAGAC 1 cut(s) 44
Bpu10I CCTNAGC 2 cut(s) 969, 975
Bpu1102I GCTNAGC 2 cut(s) 1173, 1190
Bpu14I TTCGAA 1 cut(s) 1157
BpuEI CTTGAG 2 cut(s) 923, 1114
BpuMI CCSGG 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 3 cut(s) 224, 379, 770
BsaWI WCCGGW 2 cut(s) 71, 414
BsaXI ACNNNNNCTCC 2 cut(s) 1062, 1092
Bsc4I CCNNNNNNNGG 6 cut(s) 62, 255, 697, 732, 771, 980
Bse1I ACTGG 1 cut(s) 847
Bse3DI GCAATG 1 cut(s) 1063
BseAI TCCGGA 2 cut(s) 71, 414
BseBI CCWGG 2 cut(s) 381, 785
BseDI CCNNGG 3 cut(s) 224, 379, 770
BseGI GGATG 2 cut(s) 374, 808
BseLI CCNNNNNNNGG 6 cut(s) 62, 255, 697, 732, 771, 980
BseMI GCAATG 1 cut(s) 1063
BseMII CTCAG 5 cut(s) 789, 960, 966, 1026, 1164
BseNI ACTGG 1 cut(s) 847
BseXI GCAGC 4 cut(s) 588, 591, 658, 1095
BsgI GTGCAG 2 cut(s) 202, 468
BshFI GGCC 3 cut(s) 368, 736, 788
BsiHKCI CYCGRG 3 cut(s) 890, 932, 1137
BsiSI CCGG 3 cut(s) 72, 250, 415
BslFI GGGAC 1 cut(s) 1103
BslI CCNNNNNNNGG 6 cut(s) 62, 255, 697, 732, 771, 980
BsmAI GTCTC 2 cut(s) 343, 765
BsmFI GGGAC 1 cut(s) 1103
BsmI GAATGC 1 cut(s) 505
BsnI GGCC 3 cut(s) 368, 736, 788
Bso31I GGTCTC 1 cut(s) 343
BsoBI CYCGRG 3 cut(s) 890, 932, 1137
Bsp119I TTCGAA 1 cut(s) 1157
Bsp1286I GDGCHC 2 cut(s) 891, 933
Bsp13I TCCGGA 2 cut(s) 71, 414
Bsp143I GATC 4 cut(s) 132, 143, 805, 874
Bsp1720I GCTNAGC 2 cut(s) 1173, 1190
BspANI GGCC 3 cut(s) 368, 736, 788
BspCNI CTCAG 5 cut(s) 790, 961, 967, 1025, 1165
BspEI TCCGGA 2 cut(s) 71, 414
BspLI GGNNCC 1 cut(s) 367
BspPI GGATC 2 cut(s) 151, 869
BspQI GCTCTTC 1 cut(s) 106
BspT104I TTCGAA 1 cut(s) 1157
BspTNI GGTCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 1063
BsrI ACTGG 1 cut(s) 847
BssECI CCNNGG 3 cut(s) 224, 379, 770
BssMI GATC 4 cut(s) 132, 143, 805, 874
BssT1I CCWWGG 1 cut(s) 224
Bst2UI CCWGG 2 cut(s) 381, 785
Bst4CI ACNGT 4 cut(s) 508, 572, 823, 996
Bst6I CTCTTC 2 cut(s) 70, 106
BstBAI YACGTR 1 cut(s) 180
BstBI TTCGAA 1 cut(s) 1157
BstC8I GCNNGC 3 cut(s) 160, 734, 738
BstDEI CTNAG 7 cut(s) 798, 945, 969, 975, 1012, 1173, 1190
BstENI CCTNNNNNAGG 4 cut(s) 60, 695, 730, 978
BstF5I GGATG 2 cut(s) 374, 808
BstHHI GCGC 1 cut(s) 1202
BstKTI GATC 4 cut(s) 135, 146, 808, 877
BstMAI GTCTC 2 cut(s) 343, 765
BstMBI GATC 4 cut(s) 132, 143, 805, 874
BstMWI GCNNNNNNNGC 1 cut(s) 573
BstNI CCWGG 2 cut(s) 381, 785
BstNSI RCATGY 1 cut(s) 833
BstSCI CCNGG 3 cut(s) 248, 379, 783
BstSFI CTRYAG 1 cut(s) 717
BstV1I GCAGC 4 cut(s) 588, 591, 658, 1095
BstV2I GAAGAC 1 cut(s) 44
BstX2I RGATCY 1 cut(s) 874
BstYI RGATCY 1 cut(s) 874
BsuRI GGCC 3 cut(s) 368, 736, 788
BtsCI GGATG 2 cut(s) 374, 808
BtsI GCAGTG 2 cut(s) 94, 369
BtsIMutI CAGTG 3 cut(s) 94, 369, 577
Cac8I GCNNGC 3 cut(s) 160, 734, 738
CaiI CAGNNNCTG 1 cut(s) 1034
CfoI GCGC 1 cut(s) 1202
Cfr13I GGNCC 6 cut(s) 32, 366, 383, 421, 601, 1003
Csp6I GTAC 4 cut(s) 244, 824, 1063, 1098
CviAII CATG 6 cut(s) 239, 488, 830, 863, 926, 938
CviQI GTAC 4 cut(s) 244, 824, 1063, 1098
DdeI CTNAG 7 cut(s) 798, 945, 969, 975, 1012, 1173, 1190
DpnI GATC 4 cut(s) 134, 145, 807, 876
DpnII GATC 4 cut(s) 132, 143, 805, 874
Eam1104I CTCTTC 2 cut(s) 70, 106
EarI CTCTTC 2 cut(s) 70, 106
Eco130I CCWWGG 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 736
Eco24I GRGCYC 2 cut(s) 891, 933
Eco31I GGTCTC 1 cut(s) 343
Eco47I GGWCC 5 cut(s) 32, 383, 421, 601, 1003
Eco57I CTGAAG 2 cut(s) 712, 1056
Eco88I CYCGRG 3 cut(s) 890, 932, 1137
EcoNI CCTNNNNNAGG 4 cut(s) 60, 695, 730, 978
EcoRI GAATTC 1 cut(s) 835
EcoRII CCWGG 2 cut(s) 379, 783
EcoT14I CCWWGG 1 cut(s) 224
EcoT38I GRGCYC 2 cut(s) 891, 933
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 6 cut(s) 242, 491, 833, 866, 929, 941
FalI AAGNNNNNCTT 4 cut(s) 1029, 1061, 1059, 1091
FaqI GGGAC 1 cut(s) 1103
FatI CATG 6 cut(s) 238, 487, 829, 862, 925, 937
FbaI TGATCA 1 cut(s) 805
Fnu4HI GCNGC 4 cut(s) 577, 580, 672, 1109
FokI GGATG 2 cut(s) 361, 815
FriOI GRGCYC 2 cut(s) 891, 933
Fsp4HI GCNGC 4 cut(s) 577, 580, 672, 1109
FspBI CTAG 2 cut(s) 195, 387
GlaI GCGC 1 cut(s) 1201
GluI GCNGC 4 cut(s) 577, 580, 672, 1109
HaeIII GGCC 3 cut(s) 368, 736, 788
HapII CCGG 3 cut(s) 72, 250, 415
HhaI GCGC 1 cut(s) 1202
Hin1II CATG 6 cut(s) 242, 491, 833, 866, 929, 941
Hin6I GCGC 1 cut(s) 1200
HinP1I GCGC 1 cut(s) 1200
HincII GTYRAC 2 cut(s) 777, 868
HindII GTYRAC 2 cut(s) 777, 868
HindIII AAGCTT 2 cut(s) 474, 899
HinfI GANTC 6 cut(s) 302, 427, 512, 656, 680, 859
HpaI GTTAAC 1 cut(s) 777
HpaII CCGG 3 cut(s) 72, 250, 415
HphI GGTGA 1 cut(s) 138
Hpy166II GTNNAC 2 cut(s) 777, 868
Hpy188I TCNGA 4 cut(s) 40, 307, 799, 1015
Hpy8I GTNNAC 2 cut(s) 777, 868
HpyAV CCTTC 7 cut(s) 412, 460, 691, 736, 1046, 1061, 1162
HpyCH4III ACNGT 4 cut(s) 508, 572, 823, 996
HpyCH4IV ACGT 2 cut(s) 179, 596
HpyCH4V TGCA 5 cut(s) 162, 183, 374, 449, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 573
HpyF3I CTNAG 7 cut(s) 798, 945, 969, 975, 1012, 1173, 1190
HpySE526I ACGT 2 cut(s) 179, 596
Hsp92II CATG 6 cut(s) 242, 491, 833, 866, 929, 941
HspAI GCGC 1 cut(s) 1200
Kpn2I TCCGGA 2 cut(s) 71, 414
Ksp22I TGATCA 1 cut(s) 805
KspAI GTTAAC 1 cut(s) 777
Kzo9I GATC 4 cut(s) 132, 143, 805, 874
LguI GCTCTTC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 252
Lsp1109I GCAGC 4 cut(s) 588, 591, 658, 1095
LweI GCATC 4 cut(s) 31, 383, 749, 931
MaeI CTAG 2 cut(s) 195, 387
MaeII ACGT 2 cut(s) 179, 596
MaeIII GTNAC 4 cut(s) 3, 92, 126, 350
MalI GATC 4 cut(s) 134, 145, 807, 876
MboI GATC 4 cut(s) 132, 143, 805, 874
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 2 cut(s) 891, 933
MluCI AATT 6 cut(s) 66, 616, 745, 835, 953, 1115
MlyI GAGTC 1 cut(s) 421
MmeI TCCRAC 2 cut(s) 579, 888
MroI TCCGGA 2 cut(s) 71, 414
MseI TTAA 4 cut(s) 453, 522, 530, 776
MspI CCGG 3 cut(s) 72, 250, 415
MspR9I CCNGG 3 cut(s) 250, 381, 785
Mva1269I GAATGC 1 cut(s) 505
MvaI CCWGG 2 cut(s) 381, 785
MwoI GCNNNNNNNGC 1 cut(s) 573
NciI CCSGG 1 cut(s) 250
NdeII GATC 4 cut(s) 132, 143, 805, 874
NlaIII CATG 6 cut(s) 242, 491, 833, 866, 929, 941
NlaIV GGNNCC 1 cut(s) 367
NmuCI GTSAC 2 cut(s) 92, 126
NspI RCATGY 1 cut(s) 833
NspV TTCGAA 1 cut(s) 1157
PaeR7I CTCGAG 1 cut(s) 1137
PceI AGGCCT 1 cut(s) 736
PciI ACATGT 1 cut(s) 829
PciSI GCTCTTC 1 cut(s) 106
PctI GAATGC 1 cut(s) 505
PfeI GAWTC 5 cut(s) 302, 512, 656, 680, 859
PfoI TCCNGGA 1 cut(s) 248
PkrI GCNGC 4 cut(s) 578, 581, 673, 1110
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
Ppu21I YACGTR 1 cut(s) 180
PscI ACATGT 1 cut(s) 829
Psp6I CCWGG 2 cut(s) 379, 783
PspGI CCWGG 2 cut(s) 379, 783
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 6 cut(s) 32, 366, 383, 421, 601, 1003
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 1 cut(s) 874
RsaI GTAC 4 cut(s) 245, 825, 1064, 1099
RsaNI GTAC 4 cut(s) 244, 824, 1063, 1098
SapI GCTCTTC 1 cut(s) 106
SaqAI TTAA 4 cut(s) 453, 522, 530, 776
SatI GCNGC 4 cut(s) 577, 580, 672, 1109
Sau3AI GATC 4 cut(s) 132, 143, 805, 874
Sau96I GGNCC 6 cut(s) 32, 366, 383, 421, 601, 1003
SchI GAGTC 1 cut(s) 421
ScrFI CCNGG 3 cut(s) 250, 381, 785
SduI GDGCHC 2 cut(s) 891, 933
SfaNI GCATC 4 cut(s) 31, 383, 749, 931
SfcI CTRYAG 1 cut(s) 717
Sfr274I CTCGAG 1 cut(s) 1137
SfuI TTCGAA 1 cut(s) 1157
SinI GGWCC 5 cut(s) 32, 383, 421, 601, 1003
SlaI CTCGAG 1 cut(s) 1137
SmlI CTYRAG 3 cut(s) 902, 1093, 1137
SmoI CTYRAG 3 cut(s) 902, 1093, 1137
Sse9I AATT 6 cut(s) 66, 616, 745, 835, 953, 1115
SseBI AGGCCT 1 cut(s) 736
SspMI CTAG 2 cut(s) 195, 387
StuI AGGCCT 1 cut(s) 736
StyD4I CCNGG 3 cut(s) 248, 379, 783
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 4 cut(s) 508, 572, 823, 996
TaiI ACGT 2 cut(s) 182, 599
TaqI TCGA 2 cut(s) 1138, 1157
TasI AATT 6 cut(s) 66, 616, 745, 835, 953, 1115
TatI WGTACW 1 cut(s) 1097
TfiI GAWTC 5 cut(s) 302, 512, 656, 680, 859
Tru1I TTAA 4 cut(s) 453, 522, 530, 776
Tru9I TTAA 4 cut(s) 453, 522, 530, 776
TscAI CASTG 3 cut(s) 101, 376, 577
TseFI GTSAC 2 cut(s) 92, 126
TseI GCWGC 4 cut(s) 576, 579, 671, 1108
Tsp45I GTSAC 2 cut(s) 92, 126
TspDTI ATGAA 2 cut(s) 5, 1178
TspRI CASTG 3 cut(s) 101, 376, 577
VpaK11BI GGWCC 5 cut(s) 32, 383, 421, 601, 1003
XagI CCTNNNNNAGG 4 cut(s) 60, 695, 730, 978
XapI RAATTY 4 cut(s) 66, 745, 835, 953
XceI RCATGY 1 cut(s) 833
XhoI CTCGAG 1 cut(s) 1137
XspI CTAG 2 cut(s) 195, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.