RLG00000012454

rho-N domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007951
Physical Location & Seq
Forward (+)
18948940 .. 18950879
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012454

Sequence Viewer

Length: 1206 bp
ATGTCACAGAGCGTTCATCTCATCTGTTATGGACCATCAGATAGCAAATGTCTACCTTGTTCTGGGATTTCGAGGAGGGTGGCCCCTGTATATCCTTGCCCTTCTTGTTTCAACCATAGATTCAAGTCGCAGGTCAGGGTTGGATCACTAAAAGGTGCTTATACTGGGGTGTCTTTTACATGCAGAGCAGGTTCTAGTGGTCGTAATAGAAATCCAGACTTCCCAAGGCAAAACAGGCAAGGGTATTCTCGGGGGAGGAACAGGAGAAATGAAGAGAGAGACAGCTTTGAAAACCTTGAAGAGTCTGATTTGCTTTCTGCTAAAAATGGTCCATTGCTCTCCCTCCCTAGTAACACAAAGTTTGGGGCTACTGCAGCCCCTGGTCCCAGAGAAAAAGAGATTGTTGAGCTGTTCAGGAAAGTCCAGGCTCAGCTTCGGGAGAGAACTGCTGTTAAAGAAGAAAAGAAGGTTGAACCTGTGCAAGGGCAAGGTAAAGATAATGGAACTGTGGATTCTCTTCTTAAACTACTAAGGAAACATTCGGCTGAACAGGCAAAGAGAAACAGTAATGGGGTTGGAAACAAAGACTTTATGTTGAACCAACCAGAAAAGAATGGCCGATATAGTGAAAGAAAAAACACGGCTTCTTTGGATTCAATTAACAGTGTGAAGGATTATGCTGAAGAGCCTACTGCCTCTTTGAGCAGGCCTGCATCAAATTTTCGACGCAAGTCTCCCATCAATCGATTGACATATCAACCTATTTATTCAGAGGATGATGAGATAGTCAATTCGGCGCAGCATGTGAATTCAACTGACAAAAGAAAAAATAATTATGCTGAGAGAGTTTCTAAACCAGAACCTGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCTTGAGATTGATGTGGAGTCGGTACTTGAGCCTGAGCCTGTTCACGATACTGAGTTTGGATTTGAAACTGAACCTGAGCCTGAGCAAGAGATTGTTCAGTTGTTAGAGGATGAGACTTCAGGTACTGAAGAAAATGTGGATGATGATGATGAGGACGAGAACAAAGAGCAACTTATTGAACAGATGGATTTGAGTGCATGTAAGCTGCCAGAATTGAGGGCACTTGCCAAGTCTCGAGGCGTGAAAGGGTTCTCAAAGATGAAGAAGGCCGAGCTTCTGGAGTTGCTAACTGGTGGGAGCTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

402

Amino Acids

44.96

Weight (kDa)

5.32

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 362 - 393 1.7e-06 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 121, 179
AccI GTMKAC 1 cut(s) 52
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 2 cut(s) 718, 808
AcuI CTGAAG 3 cut(s) 702, 999, 1044
AfaI GTAC 2 cut(s) 921, 1021
AfiI CCNNNNNNNGG 2 cut(s) 62, 482
AjnI CCWGG 2 cut(s) 379, 423
AluBI AGCT 7 cut(s) 285, 409, 433, 898, 1102, 1171, 1197
AluI AGCT 7 cut(s) 285, 409, 433, 898, 1102, 1171, 1197
Alw26I GTCTC 4 cut(s) 273, 738, 1004, 1134
AlwI GGATC 1 cut(s) 151
AlwNI CAGNNNCTG 3 cut(s) 380, 863, 1022
Ama87I CYCGRG 2 cut(s) 249, 1131
AoxI GGCC 4 cut(s) 81, 616, 707, 1164
ApeKI GCWGC 3 cut(s) 374, 799, 1102
ApoI RAATTY 2 cut(s) 718, 808
Asp700I GAANNNNTTC 1 cut(s) 1145
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 4 cut(s) 32, 82, 329, 383
AvaI CYCGRG 2 cut(s) 249, 1131
AvaII GGWCC 3 cut(s) 32, 329, 383
BaeGI GKGCMC 1 cut(s) 1120
BbvI GCAGC 3 cut(s) 386, 811, 1089
BccI CCATC 3 cut(s) 43, 746, 1075
BceAI ACGGC 1 cut(s) 657
BciT130I CCWGG 2 cut(s) 381, 425
BcoDI GTCTC 4 cut(s) 273, 738, 1004, 1134
BfaI CTAG 2 cut(s) 195, 348
BfmI CTRYAG 1 cut(s) 372
BfuAI ACCTGC 2 cut(s) 121, 179
BisI GCNGC 3 cut(s) 375, 800, 1103
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 3 cut(s) 376, 801, 1104
Bme1390I CCNGG 2 cut(s) 381, 425
Bme18I GGWCC 3 cut(s) 32, 329, 383
BmeT110I CYCGRG 2 cut(s) 249, 1131
BmgT120I GGNCC 4 cut(s) 32, 82, 329, 383
BmiI GGNNCC 2 cut(s) 84, 385
BmrFI CCNGG 2 cut(s) 381, 425
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 722
BmuI ACTGGG 1 cut(s) 174
BoxI GACNNNNGTC 1 cut(s) 730
BpmI CTGGAG 1 cut(s) 1196
Bpu10I CCTNAGC 4 cut(s) 864, 930, 972, 978
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 2 cut(s) 920, 944
Bsa29I ATCGAT 1 cut(s) 745
BsaJI CCNNGG 2 cut(s) 224, 379
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 482
Bse1I ACTGG 2 cut(s) 169, 1192
Bse3DI GCAATG 1 cut(s) 332
BseBI CCWGG 2 cut(s) 381, 425
BseCI ATCGAT 1 cut(s) 745
BseDI CCNNGG 2 cut(s) 224, 379
BseGI GGATG 3 cut(s) 781, 1012, 1042
BseLI CCNNNNNNNGG 2 cut(s) 62, 482
BseMI GCAATG 1 cut(s) 332
BseMII CTCAG 7 cut(s) 443, 831, 855, 921, 939, 963, 969
BseNI ACTGG 2 cut(s) 169, 1192
BseRI GAGGAG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 1120
BseXI GCAGC 3 cut(s) 386, 811, 1089
BshFI GGCC 4 cut(s) 83, 618, 709, 1166
BshVI ATCGAT 1 cut(s) 745
BsiHKCI CYCGRG 2 cut(s) 249, 1131
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 2 cut(s) 62, 482
BsmAI GTCTC 4 cut(s) 273, 738, 1004, 1134
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 4 cut(s) 83, 618, 709, 1166
BsoBI CYCGRG 2 cut(s) 249, 1131
Bsp1286I GDGCHC 1 cut(s) 1120
Bsp143I GATC 1 cut(s) 143
Bsp1720I GCTNAGC 1 cut(s) 429
BspANI GGCC 4 cut(s) 83, 618, 709, 1166
BspCNI CTCAG 7 cut(s) 442, 832, 856, 922, 940, 964, 970
BspDI ATCGAT 1 cut(s) 745
BspLI GGNNCC 2 cut(s) 84, 385
BspMAI CTGCAG 1 cut(s) 376
BspMI ACCTGC 2 cut(s) 121, 179
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 332
BsrI ACTGG 2 cut(s) 169, 1192
BssECI CCNNGG 2 cut(s) 224, 379
BssMI GATC 1 cut(s) 143
BssT1I CCWWGG 1 cut(s) 224
Bst2UI CCWGG 2 cut(s) 381, 425
Bst4CI ACNGT 3 cut(s) 508, 566, 665
Bst6I CTCTTC 4 cut(s) 267, 294, 522, 678
BstC8I GCNNGC 2 cut(s) 707, 711
BstDEI CTNAG 9 cut(s) 429, 530, 840, 864, 930, 948, 972, 978, 1198
BstENI CCTNNNNNAGG 1 cut(s) 480
BstF5I GGATG 3 cut(s) 781, 1012, 1042
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 1 cut(s) 146
BstMAI GTCTC 4 cut(s) 273, 738, 1004, 1134
BstMBI GATC 1 cut(s) 143
BstMWI GCNNNNNNNGC 3 cut(s) 235, 374, 551
BstNI CCWGG 2 cut(s) 381, 425
BstNSI RCATGY 3 cut(s) 183, 806, 1098
BstPAI GACNNNNGTC 1 cut(s) 730
BstSCI CCNGG 2 cut(s) 379, 423
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 1120
BstV1I GCAGC 3 cut(s) 386, 811, 1089
Bsu15I ATCGAT 1 cut(s) 745
BsuRI GGCC 4 cut(s) 83, 618, 709, 1166
BsuTUI ATCGAT 1 cut(s) 745
BtsCI GGATG 3 cut(s) 781, 1012, 1042
BtsIMutI CAGTG 1 cut(s) 670
BveI ACCTGC 2 cut(s) 121, 179
Cac8I GCNNGC 2 cut(s) 707, 711
CaiI CAGNNNCTG 3 cut(s) 380, 863, 1022
CfoI GCGC 1 cut(s) 799
Cfr13I GGNCC 4 cut(s) 32, 82, 329, 383
ClaI ATCGAT 1 cut(s) 745
CseI GACGC 1 cut(s) 735
Csp6I GTAC 2 cut(s) 920, 1020
CviAII CATG 3 cut(s) 180, 803, 1095
CviQI GTAC 2 cut(s) 920, 1020
DdeI CTNAG 9 cut(s) 429, 530, 840, 864, 930, 948, 972, 978, 1198
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 616
Eam1104I CTCTTC 4 cut(s) 267, 294, 522, 678
EarI CTCTTC 4 cut(s) 267, 294, 522, 678
Eco130I CCWWGG 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 709
Eco47I GGWCC 3 cut(s) 32, 329, 383
Eco57I CTGAAG 3 cut(s) 702, 999, 1044
Eco88I CYCGRG 2 cut(s) 249, 1131
EcoNI CCTNNNNNAGG 1 cut(s) 480
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 379, 423
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 3 cut(s) 183, 806, 1098
FalI AAGNNNNNCTT 2 cut(s) 1053, 1085
FaqI GGGAC 1 cut(s) 369
FatI CATG 3 cut(s) 179, 802, 1094
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 3 cut(s) 375, 800, 1103
FokI GGATG 3 cut(s) 788, 1019, 1049
Fsp4HI GCNGC 3 cut(s) 375, 800, 1103
FspBI CTAG 2 cut(s) 195, 348
GlaI GCGC 1 cut(s) 798
GluI GCNGC 3 cut(s) 375, 800, 1103
GsuI CTGGAG 1 cut(s) 1196
HaeIII GGCC 4 cut(s) 83, 618, 709, 1166
HgaI GACGC 1 cut(s) 735
HhaI GCGC 1 cut(s) 799
Hin1II CATG 3 cut(s) 183, 806, 1098
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HinfI GANTC 5 cut(s) 120, 302, 512, 653, 914
Hpy166II GTNNAC 2 cut(s) 53, 940
Hpy188I TCNGA 3 cut(s) 40, 307, 772
Hpy188III TCNNGA 6 cut(s) 215, 415, 437, 941, 1131, 1175
Hpy8I GTNNAC 2 cut(s) 53, 940
Hpy99I CGWCG 1 cut(s) 729
HpyAV CCTTC 4 cut(s) 111, 460, 664, 1156
HpyCH4III ACNGT 3 cut(s) 508, 566, 665
HpyCH4V TGCA 5 cut(s) 183, 374, 481, 713, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 374, 551
HpyF3I CTNAG 9 cut(s) 429, 530, 840, 864, 930, 948, 972, 978, 1198
Hsp92II CATG 3 cut(s) 183, 806, 1098
HspAI GCGC 1 cut(s) 797
Kzo9I GATC 1 cut(s) 143
LguI GCTCTTC 1 cut(s) 678
LmnI GCTCC 1 cut(s) 1194
Lsp1109I GCAGC 3 cut(s) 386, 811, 1089
LweI GCATC 1 cut(s) 722
MaeI CTAG 2 cut(s) 195, 348
MaeIII GTNAC 2 cut(s) 3, 350
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MboII GAAGA 7 cut(s) 284, 311, 470, 509, 695, 1037, 1171
MhlI GDGCHC 1 cut(s) 1120
MluCI AATT 6 cut(s) 657, 718, 790, 808, 832, 1109
MlyI GAGTC 2 cut(s) 311, 923
MmeI TCCRAC 2 cut(s) 121, 556
MroXI GAANNNNTTC 1 cut(s) 1145
MseI TTAA 3 cut(s) 453, 522, 660
MspR9I CCNGG 2 cut(s) 381, 425
MvaI CCWGG 2 cut(s) 381, 425
MwoI GCNNNNNNNGC 3 cut(s) 235, 374, 551
NdeII GATC 1 cut(s) 143
NlaIII CATG 3 cut(s) 183, 806, 1098
NlaIV GGNNCC 2 cut(s) 84, 385
NmeAIII GCCGAG 1 cut(s) 1192
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 3 cut(s) 183, 806, 1098
PaeR7I CTCGAG 1 cut(s) 1131
PceI AGGCCT 1 cut(s) 709
PciSI GCTCTTC 1 cut(s) 678
PdmI GAANNNNTTC 1 cut(s) 1145
PfeI GAWTC 3 cut(s) 120, 512, 653
PkrI GCNGC 3 cut(s) 376, 801, 1104
PleI GAGTC 2 cut(s) 310, 922
PpsI GAGTC 2 cut(s) 310, 922
PshAI GACNNNNGTC 1 cut(s) 730
Psp6I CCWGG 2 cut(s) 379, 423
PspGI CCWGG 2 cut(s) 379, 423
PspN4I GGNNCC 2 cut(s) 84, 385
PspPI GGNCC 4 cut(s) 32, 82, 329, 383
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 3 cut(s) 380, 863, 1022
RsaI GTAC 2 cut(s) 921, 1021
RsaNI GTAC 2 cut(s) 920, 1020
SapI GCTCTTC 1 cut(s) 678
SaqAI TTAA 3 cut(s) 453, 522, 660
SatI GCNGC 3 cut(s) 375, 800, 1103
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 4 cut(s) 32, 82, 329, 383
SchI GAGTC 2 cut(s) 311, 923
ScrFI CCNGG 2 cut(s) 381, 425
SduI GDGCHC 1 cut(s) 1120
SfaNI GCATC 1 cut(s) 722
SfcI CTRYAG 1 cut(s) 372
Sfr274I CTCGAG 1 cut(s) 1131
SinI GGWCC 3 cut(s) 32, 329, 383
SlaI CTCGAG 1 cut(s) 1131
SmlI CTYRAG 3 cut(s) 899, 923, 1131
SmoI CTYRAG 3 cut(s) 899, 923, 1131
Sse9I AATT 6 cut(s) 657, 718, 790, 808, 832, 1109
SseBI AGGCCT 1 cut(s) 709
SspMI CTAG 2 cut(s) 195, 348
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 2 cut(s) 379, 423
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 3 cut(s) 508, 566, 665
TaqI TCGA 4 cut(s) 71, 724, 745, 1132
TasI AATT 6 cut(s) 657, 718, 790, 808, 832, 1109
TfiI GAWTC 3 cut(s) 120, 512, 653
Tru1I TTAA 3 cut(s) 453, 522, 660
Tru9I TTAA 3 cut(s) 453, 522, 660
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 374, 799, 1102
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 3 cut(s) 5, 285, 1172
TspRI CASTG 1 cut(s) 670
VpaK11BI GGWCC 3 cut(s) 32, 329, 383
XagI CCTNNNNNAGG 1 cut(s) 480
XapI RAATTY 2 cut(s) 718, 808
XceI RCATGY 3 cut(s) 183, 806, 1098
XhoI CTCGAG 1 cut(s) 1131
XmiI GTMKAC 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 1145
XspI CTAG 2 cut(s) 195, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.