Prupe.8G222600_v2.0.a1

rho-N domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0007951
Physical Location & Seq
Reverse (-)
20157637 .. 20159774
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G222600.1

Sequence Viewer

Length: 1176 bp
ATGTTACAAGGCGTCCATATAATCGATGGAAAATGCCTTCCGTGTTCGGGAATTTCTGGAACAGTAGCCCCTGCATATCCTTGCTCTTCTCGTTTCAACCATAGATTCAAGTCACAGGTCAGGATTGGATCACTGAATGGTGCTTACAAGGGGGTATCTTTTACATGCAGAGCAAGTTCTAGTGGACGTAATAGAAATCCAGACTTCCCAAGGCAAAACAGACATGGGTACTCCCGGGGCAGAAATAGGAAAAATGACGATAGAGATGGATTTGAAAACCTTGAAGAATCTGATCTGCTGTCGTCTAAAAATGGGCCATTGGTCTCCCTCTCCAGTGGCACAAAGTTCGGGGCTACTGCAGCCCCTGGACCTAGAGAAAAAGAGATTGTTGAGCTGTTCAAGAAGGTCCAGGCTCAACTTCGGGAGAGAAGTGCAGTTAAAGAAGAAAAGAAGGCTGAAGCCTTGCAAGGGCAAGGAAAAGAGAATGAAACTGTGGATTCTCTCCTTAAGCTATTAAGGAAACACTCGGTTGAGCAAGCTAAAAGAACCAACAACAGTGGCAGCAACAAGGACTTCATGTTGGACCAGCCAGAAAAGAATGCCCGATATAGTGAAAGGAAAAGCACAACTTCTTTTGATTCAAATAACAGTTTGAAGGATGAGGTTGAAGAACCTAATGCCTCTTTTAGCAGGCCTGCATCGAATTTTCAACGCAAGTCTCCCATCCCTCGGTTGACATACCAGCCCATTTATTCAGAGGATGACCACATAGGCAACTCGGTGCCACATGTGAATTCAATTGGGAAGAGAAAGAAGAATCATTTTGAGAGGGTTCCTAAACCAGAGCCAGAGCCAGAACCAAAGCGAGAGCCTGAGCTTGAGTTGGAGTCAGAACTTGAGCATGAGCCTGTTGTCGATGCTGAGTTTGAATTCGAGCCTGAACCCGAGCCCGAGCCAGAGCTTGTTCAGTTGTTAGAGGAAGAGACTTCAGGTCCTGAAGAAACTCTTAATTTCGATGATGAAAACGGTGAGAAACAGCAACTTATCGAACGTAAGGATTTGAGTGCATTGAAGCTACTAGAATTAAGAGCACTTGCAAAGTCTCAAGGTGTAAAAGGGTTTTCAAAGATGAAGAAGGGTGAGCTTGTGGAGTTGCTAAGTGGGAGCTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

392

Amino Acids

43.83

Weight (kDa)

6.17

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 781
AclWI GGATC 1 cut(s) 136
AcsI RAATTY 4 cut(s) 51, 703, 793, 929
AcuI CTGAAG 3 cut(s) 477, 972, 1017
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 3 cut(s) 47, 468, 729
AflII CTTAAG 1 cut(s) 506
AflIII ACRYGT 1 cut(s) 787
AhdI GACNNNNNGTC 1 cut(s) 990
AjnI CCWGG 2 cut(s) 364, 408
AluBI AGCT 8 cut(s) 394, 511, 539, 877, 961, 1075, 1144, 1167
AluI AGCT 8 cut(s) 394, 511, 539, 877, 961, 1075, 1144, 1167
Alw21I GWGCWC 2 cut(s) 1093, 1169
Alw26I GTCTC 4 cut(s) 328, 723, 977, 1107
AlwI GGATC 1 cut(s) 136
AlwNI CAGNNNCTG 2 cut(s) 365, 995
Ama87I CYCGRG 3 cut(s) 234, 944, 950
AoxI GGCC 2 cut(s) 314, 692
ApeKI GCWGC 2 cut(s) 359, 561
ApoI RAATTY 4 cut(s) 51, 703, 793, 929
AspS9I GGNCC 5 cut(s) 314, 368, 406, 583, 992
AsuC2I CCSGG 2 cut(s) 235, 236
AsuHPI GGTGA 2 cut(s) 1040, 1151
AvaI CYCGRG 3 cut(s) 234, 944, 950
AvaII GGWCC 4 cut(s) 368, 406, 583, 992
BanI GGYRCC 1 cut(s) 781
BanII GRGCYC 2 cut(s) 951, 1169
Bbv12I GWGCWC 2 cut(s) 1093, 1169
BbvI GCAGC 2 cut(s) 371, 573
BccI CCATC 3 cut(s) 20, 260, 731
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 2 cut(s) 366, 410
BcnI CCSGG 2 cut(s) 235, 236
BcoDI GTCTC 4 cut(s) 328, 723, 977, 1107
BfaI CTAG 3 cut(s) 180, 372, 1079
BfmI CTRYAG 1 cut(s) 357
BfrI CTTAAG 1 cut(s) 506
BisI GCNGC 2 cut(s) 360, 562
BlsI GCNGC 2 cut(s) 361, 563
Bme1390I CCNGG 4 cut(s) 235, 236, 366, 410
Bme18I GGWCC 4 cut(s) 368, 406, 583, 992
BmeRI GACNNNNNGTC 1 cut(s) 990
BmeT110I CYCGRG 3 cut(s) 234, 944, 950
BmgT120I GGNCC 5 cut(s) 314, 368, 406, 583, 992
BmiI GGNNCC 2 cut(s) 783, 834
BmrFI CCNGG 4 cut(s) 235, 236, 366, 410
BmsI GCATC 2 cut(s) 707, 907
BpmI CTGGAG 1 cut(s) 316
Bpu10I CCTNAGC 1 cut(s) 873
BpuEI CTTGAG 3 cut(s) 899, 917, 1089
BpuMI CCSGG 2 cut(s) 235, 236
Bsa29I ATCGAT 1 cut(s) 24
BsaHI GRCGYC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 5 cut(s) 209, 234, 235, 364, 728
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 468, 729
Bse1I ACTGG 1 cut(s) 333
BseBI CCWGG 2 cut(s) 366, 410
BseCI ATCGAT 1 cut(s) 24
BseDI CCNNGG 5 cut(s) 209, 234, 235, 364, 728
BseGI GGATG 3 cut(s) 664, 723, 766
BseLI CCNNNNNNNGG 3 cut(s) 47, 468, 729
BseMII CTCAG 2 cut(s) 864, 912
BseNI ACTGG 1 cut(s) 333
BseXI GCAGC 2 cut(s) 371, 573
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 2 cut(s) 316, 694
BshNI GGYRCC 1 cut(s) 781
BshVI ATCGAT 1 cut(s) 24
BsiHKAI GWGCWC 2 cut(s) 1093, 1169
BsiHKCI CYCGRG 3 cut(s) 234, 944, 950
BsiSI CCGG 1 cut(s) 235
BslI CCNNNNNNNGG 3 cut(s) 47, 468, 729
BsmAI GTCTC 4 cut(s) 328, 723, 977, 1107
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 2 cut(s) 316, 694
Bso31I GGTCTC 1 cut(s) 328
BsoBI CYCGRG 3 cut(s) 234, 944, 950
Bsp1286I GDGCHC 3 cut(s) 951, 1093, 1169
Bsp143I GATC 2 cut(s) 128, 292
BspANI GGCC 2 cut(s) 316, 694
BspCNI CTCAG 2 cut(s) 865, 913
BspDI ATCGAT 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 783, 834
BspMAI CTGCAG 1 cut(s) 361
BspPI GGATC 1 cut(s) 136
BspQI GCTCTTC 1 cut(s) 91
BspT107I GGYRCC 1 cut(s) 781
BspTI CTTAAG 1 cut(s) 506
BspTNI GGTCTC 1 cut(s) 328
BsrI ACTGG 1 cut(s) 333
BssECI CCNNGG 5 cut(s) 209, 234, 235, 364, 728
BssMI GATC 2 cut(s) 128, 292
BssNI GRCGYC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 366, 410
Bst4CI ACNGT 5 cut(s) 64, 493, 557, 650, 1028
Bst6I CTCTTC 3 cut(s) 91, 800, 975
BstACI GRCGYC 1 cut(s) 12
BstAFI CTTAAG 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 537, 692, 696
BstDEI CTNAG 4 cut(s) 873, 921, 1157, 1168
BstF5I GGATG 3 cut(s) 664, 723, 766
BstKTI GATC 2 cut(s) 131, 295
BstMAI GTCTC 4 cut(s) 328, 723, 977, 1107
BstMBI GATC 2 cut(s) 128, 292
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 2 cut(s) 366, 410
BstNSI RCATGY 2 cut(s) 168, 791
BstSCI CCNGG 4 cut(s) 233, 234, 364, 408
BstSFI CTRYAG 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 371, 573
Bsu15I ATCGAT 1 cut(s) 24
BsuRI GGCC 2 cut(s) 316, 694
BsuTUI ATCGAT 1 cut(s) 24
BtsCI GGATG 3 cut(s) 664, 723, 766
BtsIMutI CAGTG 3 cut(s) 131, 340, 562
Cac8I GCNNGC 3 cut(s) 537, 692, 696
CaiI CAGNNNCTG 2 cut(s) 365, 995
Cfr13I GGNCC 5 cut(s) 314, 368, 406, 583, 992
Cfr9I CCCGGG 1 cut(s) 234
ClaI ATCGAT 1 cut(s) 24
Csp6I GTAC 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviAII CATG 5 cut(s) 165, 224, 577, 788, 902
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 4 cut(s) 873, 921, 1157, 1168
DpnI GATC 2 cut(s) 130, 294
DpnII GATC 2 cut(s) 128, 292
DriI GACNNNNNGTC 1 cut(s) 990
Eam1104I CTCTTC 3 cut(s) 91, 800, 975
Eam1105I GACNNNNNGTC 1 cut(s) 990
EarI CTCTTC 3 cut(s) 91, 800, 975
Ecl136II GAGCTC 1 cut(s) 1167
Eco130I CCWWGG 1 cut(s) 209
Eco147I AGGCCT 1 cut(s) 694
Eco24I GRGCYC 2 cut(s) 951, 1169
Eco31I GGTCTC 1 cut(s) 328
Eco47I GGWCC 4 cut(s) 368, 406, 583, 992
Eco53kI GAGCTC 1 cut(s) 1167
Eco57I CTGAAG 3 cut(s) 477, 972, 1017
Eco88I CYCGRG 3 cut(s) 234, 944, 950
EcoICRI GAGCTC 1 cut(s) 1167
EcoO109I RGGNCCY 1 cut(s) 992
EcoRI GAATTC 2 cut(s) 793, 929
EcoRII CCWGG 2 cut(s) 364, 408
EcoT14I CCWWGG 1 cut(s) 209
EcoT38I GRGCYC 2 cut(s) 951, 1169
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 5 cut(s) 168, 227, 580, 791, 905
FalI AAGNNNNNCTT 4 cut(s) 613, 645, 990, 1022
FatI CATG 5 cut(s) 164, 223, 576, 787, 901
Fnu4HI GCNGC 2 cut(s) 360, 562
FokI GGATG 3 cut(s) 671, 710, 773
FriOI GRGCYC 2 cut(s) 951, 1169
Fsp4HI GCNGC 2 cut(s) 360, 562
FspBI CTAG 3 cut(s) 180, 372, 1079
GluI GCNGC 2 cut(s) 360, 562
GsuI CTGGAG 1 cut(s) 316
HaeIII GGCC 2 cut(s) 316, 694
HapII CCGG 1 cut(s) 235
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 5 cut(s) 168, 227, 580, 791, 905
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HinfI GANTC 6 cut(s) 105, 287, 497, 638, 817, 887
HpaII CCGG 1 cut(s) 235
HphI GGTGA 2 cut(s) 1040, 1151
Hpy166II GTNNAC 2 cut(s) 185, 735
Hpy188I TCNGA 3 cut(s) 292, 757, 892
Hpy188III TCNNGA 7 cut(s) 48, 57, 121, 200, 400, 422, 995
Hpy8I GTNNAC 2 cut(s) 185, 735
HpyAV CCTTC 5 cut(s) 47, 397, 445, 649, 1129
HpyCH4III ACNGT 5 cut(s) 64, 493, 557, 650, 1028
HpyCH4IV ACGT 2 cut(s) 187, 1051
HpyCH4V TGCA 8 cut(s) 74, 168, 359, 434, 466, 698, 1067, 1097
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 4 cut(s) 873, 921, 1157, 1168
HpySE526I ACGT 2 cut(s) 187, 1051
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 5 cut(s) 168, 227, 580, 791, 905
Kzo9I GATC 2 cut(s) 128, 292
LguI GCTCTTC 1 cut(s) 91
LmnI GCTCC 1 cut(s) 1164
Lsp1109I GCAGC 2 cut(s) 371, 573
LweI GCATC 2 cut(s) 707, 907
MaeI CTAG 3 cut(s) 180, 372, 1079
MaeII ACGT 2 cut(s) 187, 1051
MaeIII GTNAC 2 cut(s) 3, 111
MalI GATC 2 cut(s) 130, 294
MboI GATC 2 cut(s) 128, 292
MboII GAAGA 9 cut(s) 78, 296, 455, 680, 817, 826, 992, 1010, 1144
MfeI CAATTG 1 cut(s) 798
MhlI GDGCHC 3 cut(s) 951, 1093, 1169
MluCI AATT 7 cut(s) 51, 703, 793, 798, 929, 1009, 1082
MlyI GAGTC 1 cut(s) 896
MmeI TCCRAC 2 cut(s) 561, 864
MnlI CCTC 7 cut(s) 338, 655, 691, 738, 751, 822, 970
MseI TTAA 5 cut(s) 438, 507, 515, 1008, 1085
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 1 cut(s) 235
MspR9I CCNGG 4 cut(s) 235, 236, 366, 410
MunI CAATTG 1 cut(s) 798
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 2 cut(s) 366, 410
MwoI GCNNNNNNNGC 1 cut(s) 359
NciI CCSGG 2 cut(s) 235, 236
NdeII GATC 2 cut(s) 128, 292
NlaIII CATG 5 cut(s) 168, 227, 580, 791, 905
NlaIV GGNNCC 2 cut(s) 783, 834
NmuCI GTSAC 1 cut(s) 111
NspI RCATGY 2 cut(s) 168, 791
PceI AGGCCT 1 cut(s) 694
PciI ACATGT 1 cut(s) 787
PciSI GCTCTTC 1 cut(s) 91
PctI GAATGC 1 cut(s) 604
PfeI GAWTC 5 cut(s) 105, 287, 497, 638, 817
PkrI GCNGC 2 cut(s) 361, 563
PleI GAGTC 1 cut(s) 895
PpsI GAGTC 1 cut(s) 895
PpuMI RGGWCCY 1 cut(s) 992
PscI ACATGT 1 cut(s) 787
Psp124BI GAGCTC 1 cut(s) 1169
Psp5II RGGWCCY 1 cut(s) 992
Psp6I CCWGG 2 cut(s) 364, 408
PspGI CCWGG 2 cut(s) 364, 408
PspN4I GGNNCC 2 cut(s) 783, 834
PspPI GGNCC 5 cut(s) 314, 368, 406, 583, 992
PspPPI RGGWCCY 1 cut(s) 992
PstI CTGCAG 1 cut(s) 361
PstNI CAGNNNCTG 2 cut(s) 365, 995
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SacI GAGCTC 1 cut(s) 1169
SapI GCTCTTC 1 cut(s) 91
SaqAI TTAA 5 cut(s) 438, 507, 515, 1008, 1085
SatI GCNGC 2 cut(s) 360, 562
Sau3AI GATC 2 cut(s) 128, 292
Sau96I GGNCC 5 cut(s) 314, 368, 406, 583, 992
SchI GAGTC 1 cut(s) 896
ScrFI CCNGG 4 cut(s) 235, 236, 366, 410
SduI GDGCHC 3 cut(s) 951, 1093, 1169
SfaNI GCATC 2 cut(s) 707, 907
SfcI CTRYAG 1 cut(s) 357
SinI GGWCC 4 cut(s) 368, 406, 583, 992
SmaI CCCGGG 1 cut(s) 236
SmlI CTYRAG 4 cut(s) 506, 878, 896, 1104
SmoI CTYRAG 4 cut(s) 506, 878, 896, 1104
Sse9I AATT 7 cut(s) 51, 703, 793, 798, 929, 1009, 1082
SseBI AGGCCT 1 cut(s) 694
SspMI CTAG 3 cut(s) 180, 372, 1079
SstI GAGCTC 1 cut(s) 1169
StuI AGGCCT 1 cut(s) 694
StyD4I CCNGG 4 cut(s) 233, 234, 364, 408
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 5 cut(s) 64, 493, 557, 650, 1028
TaiI ACGT 2 cut(s) 190, 1054
TaqI TCGA 6 cut(s) 24, 701, 915, 933, 1014, 1047
TasI AATT 7 cut(s) 51, 703, 793, 798, 929, 1009, 1082
TfiI GAWTC 5 cut(s) 105, 287, 497, 638, 817
Tru1I TTAA 5 cut(s) 438, 507, 515, 1008, 1085
Tru9I TTAA 5 cut(s) 438, 507, 515, 1008, 1085
TscAI CASTG 3 cut(s) 138, 340, 562
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 2 cut(s) 359, 561
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 4 cut(s) 501, 565, 1035, 1145
TspGWI ACGGA 1 cut(s) 30
TspMI CCCGGG 1 cut(s) 234
TspRI CASTG 3 cut(s) 138, 340, 562
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 4 cut(s) 368, 406, 583, 992
XapI RAATTY 4 cut(s) 51, 703, 793, 929
XceI RCATGY 2 cut(s) 168, 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XmaI CCCGGG 1 cut(s) 234
XspI CTAG 3 cut(s) 180, 372, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.