Rroxscaffold_7G00180650

rho-N domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007951
Physical Location & Seq
Forward (+)
19978394 .. 19980789
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00180650.1

Sequence Viewer

Length: 1140 bp
ATGTCTACCTTGTTCTGGGATTTCGGGGAGAGTAGCCCTGTCTATCCTTGCTCTTCTCGTTTCAACCATAGATTCAAGTCGCAGGTCAGGGTTGGATCACTAAAAGGTGCTTATACTGGGGTGTCTTTTACGTGCAGAGCAGGTTCTAGTGGTCGTAATAGAAATCCAGACTTCCCAAGGCAAAACAGGCAAGGGTACTCTCGGGGGAGGAACAGGAGAAATGAAGAGAGAGACAGCTTTGAAAACCTGGAAGAATCTGATTTGCTTTCTGCTAAAAATGGTCCATTGCTCTCCCTCCCTAGTAACACAAAGTTTGGGGCTACTGCAGCCCCTGGTCCCAGAGAAAAAGAGATAGTTGAGCTGTTCAGGAAAGTCCAGGCTCAGCTTCGGGAGAGAACTGCTGTTAAAGAAGAAAAGAAGGTTGAACCCGTGCAAGGGCAAGGTAAAGATAATGGAACTGTGGATTCTCTTCTTAAACTACTAAGGAAACATTCGGCTGAACAGGCAAAGAGAAACAGTAGTGGGGTTGGAAACAAAGACTTTATGTTGGACCAACCAGAAAAGACTGGCCGATATAGTGAAAGAAAAAACACGGCTTCTTTGGATTCAATTAACAGTGTGAAGGATTATGCTGAAGAGCCTACTGCCTCTTTGAGCAGGCCTGCATCAAATTTTCGACGCAAGTCTCCCATCAATCGATTGACATATCAACCTATTTATTCAGAGGATGATGAGATAGTCAATTCGGTGCAGCATGTGAATTCAACTGACAAAAGAAAAAAGAATTATGCTGAGAGAGTTTCTGAACCAGAACCTGAGCCAGAGCCAGAGCTTGAGATTGATGTGGAGTTGGTACTTGAGCCTGAGCCTGTTCATGATACTGAGTTTGGATTTGAACCTGAACCCGAGCCTGAGCAAGAGATTGTTCAGTTGTTAGAGGATGAGACTTTAGGTACTGAAGAACATGTGGATGATGATGATGAGGACGAAGACAAAGAGCAACTTATTGAACAGACGGATTTGAGTGGATGTAAGCTGCCAGAATTGAGGGCACTTGCCAAGTCTCGAGGCGTGAAAGGGTTCTCAAAGATGAGGAAGGCCGAGCTTCTGGAGTTGCTAACTGGTGGGAGCTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

379

Amino Acids

42.79

Weight (kDa)

5.16

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 341 - 371 9.4e-07 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 73, 131
AccI GTMKAC 1 cut(s) 5
AclWI GGATC 1 cut(s) 103
AcoI YGGCCR 1 cut(s) 568
AcsI RAATTY 2 cut(s) 670, 760
AcuI CTGAAG 2 cut(s) 654, 978
AfaI GTAC 3 cut(s) 197, 855, 955
AfiI CCNNNNNNNGG 3 cut(s) 15, 434, 435
AflIII ACRYGT 1 cut(s) 964
AgsI TTSAA 8 cut(s) 64, 76, 242, 425, 609, 765, 896, 1010
AjnI CCWGG 3 cut(s) 246, 331, 375
AluBI AGCT 7 cut(s) 237, 361, 385, 832, 1036, 1105, 1131
AluI AGCT 7 cut(s) 237, 361, 385, 832, 1036, 1105, 1131
Alw26I GTCTC 4 cut(s) 225, 690, 938, 1068
AlwI GGATC 1 cut(s) 103
AlwNI CAGNNNCTG 2 cut(s) 332, 815
Ama87I CYCGRG 3 cut(s) 201, 905, 1065
AoxI GGCC 3 cut(s) 568, 659, 1098
ApeKI GCWGC 3 cut(s) 326, 751, 1036
ApoI RAATTY 2 cut(s) 670, 760
Asp700I GAANNNNTTC 1 cut(s) 1079
AspS9I GGNCC 3 cut(s) 281, 335, 550
AvaI CYCGRG 3 cut(s) 201, 905, 1065
AvaII GGWCC 3 cut(s) 281, 335, 550
BaeGI GKGCMC 1 cut(s) 1054
BbsI GAAGAC 1 cut(s) 996
BbvI GCAGC 3 cut(s) 338, 763, 1023
BccI CCATC 1 cut(s) 698
BceAI ACGGC 1 cut(s) 609
BciT130I CCWGG 3 cut(s) 248, 333, 377
BcoDI GTCTC 4 cut(s) 225, 690, 938, 1068
BfaI CTAG 2 cut(s) 147, 300
BfmI CTRYAG 1 cut(s) 324
BfuAI ACCTGC 2 cut(s) 73, 131
BisI GCNGC 3 cut(s) 327, 752, 1037
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 3 cut(s) 328, 753, 1038
Bme1390I CCNGG 3 cut(s) 248, 333, 377
Bme18I GGWCC 3 cut(s) 281, 335, 550
BmeT110I CYCGRG 3 cut(s) 201, 905, 1065
BmgT120I GGNCC 3 cut(s) 281, 335, 550
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 3 cut(s) 248, 333, 377
BmrI ACTGGG 1 cut(s) 126
BmsI GCATC 1 cut(s) 674
BmuI ACTGGG 1 cut(s) 126
BoxI GACNNNNGTC 1 cut(s) 682
BpiI GAAGAC 1 cut(s) 996
BpmI CTGGAG 1 cut(s) 1130
Bpu10I CCTNAGC 3 cut(s) 816, 864, 912
Bpu1102I GCTNAGC 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 854, 878
Bsa29I ATCGAT 1 cut(s) 697
BsaAI YACGTR 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 176, 331
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 434, 435
Bse1I ACTGG 3 cut(s) 121, 571, 1126
Bse3DI GCAATG 1 cut(s) 284
BseBI CCWGG 3 cut(s) 248, 333, 377
BseCI ATCGAT 1 cut(s) 697
BseDI CCNNGG 2 cut(s) 176, 331
BseGI GGATG 4 cut(s) 733, 946, 976, 1034
BseLI CCNNNNNNNGG 3 cut(s) 15, 434, 435
BseMI GCAATG 1 cut(s) 284
BseMII CTCAG 6 cut(s) 395, 783, 807, 855, 873, 903
BseNI ACTGG 3 cut(s) 121, 571, 1126
BseSI GKGCMC 1 cut(s) 1054
BseXI GCAGC 3 cut(s) 338, 763, 1023
BsgI GTGCAG 2 cut(s) 154, 770
BshFI GGCC 3 cut(s) 570, 661, 1100
BshVI ATCGAT 1 cut(s) 697
BsiHKCI CYCGRG 3 cut(s) 201, 905, 1065
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 3 cut(s) 15, 434, 435
BsmAI GTCTC 4 cut(s) 225, 690, 938, 1068
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 3 cut(s) 570, 661, 1100
BsoBI CYCGRG 3 cut(s) 201, 905, 1065
Bsp1286I GDGCHC 1 cut(s) 1054
Bsp143I GATC 1 cut(s) 95
Bsp1720I GCTNAGC 1 cut(s) 381
BspANI GGCC 3 cut(s) 570, 661, 1100
BspCNI CTCAG 6 cut(s) 394, 784, 808, 856, 874, 904
BspDI ATCGAT 1 cut(s) 697
BspHI TCATGA 1 cut(s) 874
BspLI GGNNCC 1 cut(s) 337
BspMAI CTGCAG 1 cut(s) 328
BspMI ACCTGC 2 cut(s) 73, 131
BspPI GGATC 1 cut(s) 103
BspQI GCTCTTC 2 cut(s) 58, 630
BsrDI GCAATG 1 cut(s) 284
BsrI ACTGG 3 cut(s) 121, 571, 1126
BssECI CCNNGG 2 cut(s) 176, 331
BssMI GATC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 3 cut(s) 248, 333, 377
Bst4CI ACNGT 3 cut(s) 460, 518, 617
Bst6I CTCTTC 4 cut(s) 58, 219, 474, 630
BstBAI YACGTR 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 659, 663
BstDEI CTNAG 8 cut(s) 381, 482, 792, 816, 864, 882, 912, 1132
BstF5I GGATG 4 cut(s) 733, 946, 976, 1034
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 4 cut(s) 225, 690, 938, 1068
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 3 cut(s) 187, 326, 503
BstNI CCWGG 3 cut(s) 248, 333, 377
BstNSI RCATGY 2 cut(s) 758, 968
BstPAI GACNNNNGTC 1 cut(s) 682
BstSCI CCNGG 3 cut(s) 246, 331, 375
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 1054
BstV1I GCAGC 3 cut(s) 338, 763, 1023
BstV2I GAAGAC 1 cut(s) 996
Bsu15I ATCGAT 1 cut(s) 697
BsuRI GGCC 3 cut(s) 570, 661, 1100
BsuTUI ATCGAT 1 cut(s) 697
BtsCI GGATG 4 cut(s) 733, 946, 976, 1034
BtsIMutI CAGTG 1 cut(s) 622
BveI ACCTGC 2 cut(s) 73, 131
Cac8I GCNNGC 2 cut(s) 659, 663
CaiI CAGNNNCTG 2 cut(s) 332, 815
CciI TCATGA 1 cut(s) 874
Cfr13I GGNCC 3 cut(s) 281, 335, 550
ClaI ATCGAT 1 cut(s) 697
CseI GACGC 1 cut(s) 687
Csp6I GTAC 3 cut(s) 196, 854, 954
CviAII CATG 3 cut(s) 755, 875, 965
CviQI GTAC 3 cut(s) 196, 854, 954
DdeI CTNAG 8 cut(s) 381, 482, 792, 816, 864, 882, 912, 1132
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
EaeI YGGCCR 1 cut(s) 568
Eam1104I CTCTTC 4 cut(s) 58, 219, 474, 630
EarI CTCTTC 4 cut(s) 58, 219, 474, 630
Eco130I CCWWGG 1 cut(s) 176
Eco147I AGGCCT 1 cut(s) 661
Eco47I GGWCC 3 cut(s) 281, 335, 550
Eco57I CTGAAG 2 cut(s) 654, 978
Eco88I CYCGRG 3 cut(s) 201, 905, 1065
EcoRI GAATTC 1 cut(s) 760
EcoRII CCWGG 3 cut(s) 246, 331, 375
EcoT14I CCWWGG 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 3 cut(s) 758, 878, 968
FalI AAGNNNNNCTT 2 cut(s) 987, 1019
FaqI GGGAC 1 cut(s) 321
FatI CATG 3 cut(s) 754, 874, 964
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 327, 752, 1037
FokI GGATG 4 cut(s) 740, 953, 983, 1041
Fsp4HI GCNGC 3 cut(s) 327, 752, 1037
FspBI CTAG 2 cut(s) 147, 300
GluI GCNGC 3 cut(s) 327, 752, 1037
GsuI CTGGAG 1 cut(s) 1130
HaeIII GGCC 3 cut(s) 570, 661, 1100
HgaI GACGC 1 cut(s) 687
Hin1II CATG 3 cut(s) 758, 878, 968
HinfI GANTC 4 cut(s) 72, 254, 464, 605
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 259, 724, 805
Hpy188III TCNNGA 6 cut(s) 167, 367, 389, 875, 1065, 1109
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 681
HpyAV CCTTC 3 cut(s) 412, 616, 1090
HpyCH4III ACNGT 3 cut(s) 460, 518, 617
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 5 cut(s) 135, 326, 433, 665, 751
HpyF10VI GCNNNNNNNGC 3 cut(s) 187, 326, 503
HpyF3I CTNAG 8 cut(s) 381, 482, 792, 816, 864, 882, 912, 1132
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 3 cut(s) 758, 878, 968
Kzo9I GATC 1 cut(s) 95
LguI GCTCTTC 2 cut(s) 58, 630
LmnI GCTCC 1 cut(s) 1128
Lsp1109I GCAGC 3 cut(s) 338, 763, 1023
LweI GCATC 1 cut(s) 674
MaeI CTAG 2 cut(s) 147, 300
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 1 cut(s) 302
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 8 cut(s) 45, 236, 263, 422, 461, 647, 971, 1001
MhlI GDGCHC 1 cut(s) 1054
MluCI AATT 6 cut(s) 609, 670, 742, 760, 784, 1043
MmeI TCCRAC 3 cut(s) 73, 508, 528
MnlI CCTC 9 cut(s) 201, 305, 658, 718, 931, 976, 1041, 1061, 1086
MroXI GAANNNNTTC 1 cut(s) 1079
MseI TTAA 3 cut(s) 405, 474, 612
MslI CAYNNNNRTG 1 cut(s) 969
MspR9I CCNGG 3 cut(s) 248, 333, 377
MvaI CCWGG 3 cut(s) 248, 333, 377
MwoI GCNNNNNNNGC 3 cut(s) 187, 326, 503
NdeII GATC 1 cut(s) 95
NlaIII CATG 3 cut(s) 758, 878, 968
NlaIV GGNNCC 1 cut(s) 337
NmeAIII GCCGAG 1 cut(s) 1126
NspI RCATGY 2 cut(s) 758, 968
PaeR7I CTCGAG 1 cut(s) 1065
PagI TCATGA 1 cut(s) 874
PceI AGGCCT 1 cut(s) 661
PciI ACATGT 1 cut(s) 964
PciSI GCTCTTC 2 cut(s) 58, 630
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 4 cut(s) 72, 254, 464, 605
PkrI GCNGC 3 cut(s) 328, 753, 1038
Ppu21I YACGTR 1 cut(s) 132
PscI ACATGT 1 cut(s) 964
PshAI GACNNNNGTC 1 cut(s) 682
Psp6I CCWGG 3 cut(s) 246, 331, 375
PspGI CCWGG 3 cut(s) 246, 331, 375
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 3 cut(s) 281, 335, 550
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 2 cut(s) 332, 815
RsaI GTAC 3 cut(s) 197, 855, 955
RsaNI GTAC 3 cut(s) 196, 854, 954
RseI CAYNNNNRTG 1 cut(s) 969
SapI GCTCTTC 2 cut(s) 58, 630
SaqAI TTAA 3 cut(s) 405, 474, 612
SatI GCNGC 3 cut(s) 327, 752, 1037
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 3 cut(s) 281, 335, 550
ScrFI CCNGG 3 cut(s) 248, 333, 377
SduI GDGCHC 1 cut(s) 1054
SfaNI GCATC 1 cut(s) 674
SfcI CTRYAG 1 cut(s) 324
Sfr274I CTCGAG 1 cut(s) 1065
SinI GGWCC 3 cut(s) 281, 335, 550
SlaI CTCGAG 1 cut(s) 1065
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 3 cut(s) 833, 857, 1065
SmoI CTYRAG 3 cut(s) 833, 857, 1065
Sse9I AATT 6 cut(s) 609, 670, 742, 760, 784, 1043
SseBI AGGCCT 1 cut(s) 661
SspMI CTAG 2 cut(s) 147, 300
StuI AGGCCT 1 cut(s) 661
StyD4I CCNGG 3 cut(s) 246, 331, 375
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 3 cut(s) 460, 518, 617
TaiI ACGT 1 cut(s) 134
TaqI TCGA 3 cut(s) 676, 697, 1066
TasI AATT 6 cut(s) 609, 670, 742, 760, 784, 1043
TfiI GAWTC 4 cut(s) 72, 254, 464, 605
Tru1I TTAA 3 cut(s) 405, 474, 612
Tru9I TTAA 3 cut(s) 405, 474, 612
TscAI CASTG 1 cut(s) 622
TseI GCWGC 3 cut(s) 326, 751, 1036
TspDTI ATGAA 2 cut(s) 237, 863
TspGWI ACGGA 1 cut(s) 1031
TspRI CASTG 1 cut(s) 622
VpaK11BI GGWCC 3 cut(s) 281, 335, 550
XapI RAATTY 2 cut(s) 670, 760
XceI RCATGY 2 cut(s) 758, 968
XhoI CTCGAG 1 cut(s) 1065
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 1079
XspI CTAG 2 cut(s) 147, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.