MD10G1183500.v1.1

rho-N domain-containing protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007951
Physical Location & Seq
Reverse (-)
27660352 .. 27662630
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1183500.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGTTACAGGGCGTTCATATCATCGGTTATGGACCATCAGATGCCAATTGTCTTCCGTGTTCTGGAATTTCCGGGAGAGTAGCCCCTTTATATCCCTGCTCTTCTCGTTTCAACCATAAGTCCAAGTCACAGGTCAGTTATGGATCACCGAAAGGTGCTTACAAGGGGGTATCCTTTATGTGCAGAGCGAGTTCTAGTGGTCGTAATAGAAATCCAGAGTTTCCAAGGCAAAACAGACATGGGTACTCCCGGAGCAGGAATAGGAAAAGTGAAGAAAGAGATGGCTTTGATAACTTTGATGAATCTGATATGCTGTCTTCTAAAAATGGGCCATCGGTCTCCCTTTCAAGTAACACAAAGTTCGGGGCCACTGCATCCCCTGGACCTAGAGAAAAAGAGATTGTTGAGCTGTTCCGGAAGGTCCAGGCTCAACTTCGGGAGAGAAGTGCAGTTAAAGAAGAAAGGAAGGTTGAAGCTTCACAAGGGCATGGTAAAGAGAATGCAACTGTGGATTCCCTCCTTAACCTGTTAAGAAAGCACTCGGTTGAGCAATCAAAGAGAAACAGCAACAGTGGCAGCAGCAGCAACAAGGACTTCACGTTGGACCAACCAGAAAAGAATGCCCGATATGGTGAAAGAAAAAACACAGCTTCTTTTGATTCAAATAACAAAGCTCCTTTTGATTCAAATAACCTTTTGAAGGATGATGTTGAAGAACCTACTGCCTCCTTCAGCAGGCCTGCATCGAATTTCCAACGCAAGTCTCCCATTCCTCGGTTAACATACCAGGCCATTTATTCTGAGGATGATCACACAGGCAACTTGGTGCCACATGTGAATTCAACTGGTAAAAGAAAGAAGAATCATGTTGAGAGAGTTCCTAAACCAGAGCCTGAGCCAGAGCTTGAGTTGGAGGCCGAACTTGACCCTATGCCCGAGCATGATACTGAGTTTGAATTTGAGCCTGAACCTGAGCCGGAGCCTGAGATTGTTCAGTTGTTAGAGGACGAGACTTCAGAAGATGAGATTTTGGGTCCTGAAGAACCTCTAATTGAAGACAATGAGAACAAAGGAGAACGTGTTGAAAGTAGGGATTTGAGTACATTGAAACTGCCAGAATTGAGGGTACTAGCGAAGTCTCGAGGTGTAAAAGGGTATTCGAAGATGAAGAAGGCTGAGCTTTCGGAGTTGCTAAGCGGGAACACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rho_N

Protein Analysis

404

Amino Acids

45.05

Weight (kDa)

5.98

Isoelectric Point (pI)

54.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 365 - 395 4.1e-07 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 826
AccIII TCCGGA 1 cut(s) 414
AciI CCGC 1 cut(s) 1197
AclWI GGATC 1 cut(s) 151
AcsI RAATTY 4 cut(s) 66, 748, 838, 956
AcuI CTGAAG 3 cut(s) 715, 999, 1059
AfaI GTAC 3 cut(s) 245, 1102, 1128
AfiI CCNNNNNNNGG 5 cut(s) 62, 255, 700, 735, 774
AflIII ACRYGT 2 cut(s) 832, 1078
AjnI CCWGG 3 cut(s) 379, 423, 786
AluBI AGCT 6 cut(s) 409, 476, 650, 674, 904, 1180
AluI AGCT 6 cut(s) 409, 476, 650, 674, 904, 1180
Alw26I GTCTC 4 cut(s) 343, 768, 1004, 1143
AlwI GGATC 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 893
Ama87I CYCGRG 2 cut(s) 935, 1140
Aor13HI TCCGGA 1 cut(s) 414
AoxI GGCC 5 cut(s) 329, 366, 737, 789, 915
ApeKI GCWGC 3 cut(s) 576, 579, 582
ApoI RAATTY 4 cut(s) 66, 748, 838, 956
AspS9I GGNCC 7 cut(s) 32, 329, 366, 383, 421, 604, 1034
AsuC2I CCSGG 2 cut(s) 73, 250
AsuHPI GGTGA 2 cut(s) 138, 644
AsuII TTCGAA 1 cut(s) 1160
AvaI CYCGRG 2 cut(s) 935, 1140
AvaII GGWCC 5 cut(s) 32, 383, 421, 604, 1034
BanI GGYRCC 1 cut(s) 826
BbsI GAAGAC 3 cut(s) 44, 309, 1062
BbvI GCAGC 3 cut(s) 588, 591, 594
BccI CCATC 3 cut(s) 43, 275, 340
BciT130I CCWGG 3 cut(s) 381, 425, 788
BciVI GTATCC 1 cut(s) 181
BclI TGATCA 1 cut(s) 808
BcnI CCSGG 2 cut(s) 73, 250
BcoDI GTCTC 4 cut(s) 343, 768, 1004, 1143
BfaI CTAG 3 cut(s) 195, 387, 1130
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 3 cut(s) 577, 580, 583
BlpI GCTNAGC 2 cut(s) 1176, 1193
BlsI GCNGC 3 cut(s) 578, 581, 584
Bme1390I CCNGG 5 cut(s) 73, 250, 381, 425, 788
Bme18I GGWCC 5 cut(s) 32, 383, 421, 604, 1034
BmeT110I CYCGRG 2 cut(s) 935, 1140
BmgT120I GGNCC 7 cut(s) 32, 329, 366, 383, 421, 604, 1034
BmiI GGNNCC 4 cut(s) 367, 828, 981, 1035
BmrFI CCNGG 5 cut(s) 73, 250, 381, 425, 788
BmsI GCATC 3 cut(s) 31, 383, 752
BpiI GAAGAC 3 cut(s) 44, 309, 1062
Bpu10I CCTNAGC 2 cut(s) 894, 972
Bpu1102I GCTNAGC 2 cut(s) 1176, 1193
Bpu14I TTCGAA 1 cut(s) 1160
BpuEI CTTGAG 1 cut(s) 926
BpuMI CCSGG 2 cut(s) 73, 250
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 3 cut(s) 224, 379, 773
BsaWI WCCGGW 1 cut(s) 414
BsaXI ACNNNNNCTCC 2 cut(s) 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 255, 700, 735, 774
Bse1I ACTGG 1 cut(s) 850
BseAI TCCGGA 1 cut(s) 414
BseBI CCWGG 3 cut(s) 381, 425, 788
BseDI CCNNGG 3 cut(s) 224, 379, 773
BseGI GGATG 3 cut(s) 374, 709, 811
BseLI CCNNNNNNNGG 5 cut(s) 62, 255, 700, 735, 774
BseMII CTCAG 6 cut(s) 792, 885, 939, 963, 975, 1167
BseNI ACTGG 1 cut(s) 850
BseXI GCAGC 3 cut(s) 588, 591, 594
BsgI GTGCAG 2 cut(s) 202, 468
BshFI GGCC 5 cut(s) 331, 368, 739, 791, 917
BshNI GGYRCC 1 cut(s) 826
BsiHKCI CYCGRG 2 cut(s) 935, 1140
BsiSI CCGG 4 cut(s) 72, 250, 415, 977
BslI CCNNNNNNNGG 5 cut(s) 62, 255, 700, 735, 774
BsmAI GTCTC 4 cut(s) 343, 768, 1004, 1143
BsmI GAATGC 2 cut(s) 505, 625
BsnI GGCC 5 cut(s) 331, 368, 739, 791, 917
Bso31I GGTCTC 1 cut(s) 343
BsoBI CYCGRG 2 cut(s) 935, 1140
Bsp119I TTCGAA 1 cut(s) 1160
Bsp13I TCCGGA 1 cut(s) 414
Bsp143I GATC 2 cut(s) 143, 808
Bsp1720I GCTNAGC 2 cut(s) 1176, 1193
BspACI CCGC 1 cut(s) 1197
BspANI GGCC 5 cut(s) 331, 368, 739, 791, 917
BspCNI CTCAG 6 cut(s) 793, 886, 940, 964, 976, 1168
BspEI TCCGGA 1 cut(s) 414
BspLI GGNNCC 4 cut(s) 367, 828, 981, 1035
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 1 cut(s) 106
BspT104I TTCGAA 1 cut(s) 1160
BspT107I GGYRCC 1 cut(s) 826
BspTNI GGTCTC 1 cut(s) 343
BsrI ACTGG 1 cut(s) 850
BssECI CCNNGG 3 cut(s) 224, 379, 773
BssMI GATC 2 cut(s) 143, 808
BssT1I CCWWGG 1 cut(s) 224
Bst2UI CCWGG 3 cut(s) 381, 425, 788
Bst4CI ACNGT 3 cut(s) 508, 572, 1207
Bst6I CTCTTC 1 cut(s) 106
BstBI TTCGAA 1 cut(s) 1160
BstC8I GCNNGC 2 cut(s) 737, 741
BstDEI CTNAG 7 cut(s) 801, 894, 948, 972, 984, 1176, 1193
BstENI CCTNNNNNAGG 2 cut(s) 698, 733
BstF5I GGATG 3 cut(s) 374, 709, 811
BstKTI GATC 2 cut(s) 146, 811
BstMAI GTCTC 4 cut(s) 343, 768, 1004, 1143
BstMBI GATC 2 cut(s) 143, 808
BstMWI GCNNNNNNNGC 2 cut(s) 573, 582
BstNI CCWGG 3 cut(s) 381, 425, 788
BstNSI RCATGY 1 cut(s) 836
BstSCI CCNGG 5 cut(s) 71, 248, 379, 423, 786
BstV1I GCAGC 3 cut(s) 588, 591, 594
BstV2I GAAGAC 3 cut(s) 44, 309, 1062
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 5 cut(s) 331, 368, 739, 791, 917
BtsCI GGATG 3 cut(s) 374, 709, 811
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 369, 577
Cac8I GCNNGC 2 cut(s) 737, 741
CaiI CAGNNNCTG 1 cut(s) 893
Cfr13I GGNCC 7 cut(s) 32, 329, 366, 383, 421, 604, 1034
Csp6I GTAC 3 cut(s) 244, 1101, 1127
CviAII CATG 5 cut(s) 239, 488, 833, 866, 941
CviQI GTAC 3 cut(s) 244, 1101, 1127
DdeI CTNAG 7 cut(s) 801, 894, 948, 972, 984, 1176, 1193
DpnI GATC 2 cut(s) 145, 810
DpnII GATC 2 cut(s) 143, 808
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 739
Eco31I GGTCTC 1 cut(s) 343
Eco47I GGWCC 5 cut(s) 32, 383, 421, 604, 1034
Eco57I CTGAAG 3 cut(s) 715, 999, 1059
Eco88I CYCGRG 2 cut(s) 935, 1140
EcoNI CCTNNNNNAGG 2 cut(s) 698, 733
EcoO109I RGGNCCY 1 cut(s) 1034
EcoRI GAATTC 1 cut(s) 838
EcoRII CCWGG 3 cut(s) 379, 423, 786
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 5 cut(s) 242, 491, 836, 869, 944
FatI CATG 5 cut(s) 238, 487, 832, 865, 940
FauI CCCGC 1 cut(s) 1190
FbaI TGATCA 1 cut(s) 808
Fnu4HI GCNGC 3 cut(s) 577, 580, 583
FokI GGATG 3 cut(s) 361, 716, 818
Fsp4HI GCNGC 3 cut(s) 577, 580, 583
FspBI CTAG 3 cut(s) 195, 387, 1130
GluI GCNGC 3 cut(s) 577, 580, 583
HaeIII GGCC 5 cut(s) 331, 368, 739, 791, 917
HapII CCGG 4 cut(s) 72, 250, 415, 977
Hin1II CATG 5 cut(s) 242, 491, 836, 869, 944
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 5 cut(s) 302, 512, 659, 683, 862
HpaI GTTAAC 1 cut(s) 780
HpaII CCGG 4 cut(s) 72, 250, 415, 977
HphI GGTGA 2 cut(s) 138, 644
Hpy166II GTNNAC 1 cut(s) 780
Hpy188I TCNGA 5 cut(s) 40, 307, 802, 1018, 1186
Hpy188III TCNNGA 6 cut(s) 63, 215, 415, 437, 1037, 1140
Hpy8I GTNNAC 1 cut(s) 780
HpyAV CCTTC 5 cut(s) 412, 460, 694, 739, 1165
HpyCH4III ACNGT 3 cut(s) 508, 572, 1207
HpyCH4IV ACGT 2 cut(s) 599, 1078
HpyCH4V TGCA 5 cut(s) 183, 374, 449, 503, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 573, 582
HpyF3I CTNAG 7 cut(s) 801, 894, 948, 972, 984, 1176, 1193
HpySE526I ACGT 2 cut(s) 599, 1078
Hsp92II CATG 5 cut(s) 242, 491, 836, 869, 944
Kpn2I TCCGGA 1 cut(s) 414
Ksp22I TGATCA 1 cut(s) 808
KspAI GTTAAC 1 cut(s) 780
Kzo9I GATC 2 cut(s) 143, 808
LguI GCTCTTC 1 cut(s) 106
LmnI GCTCC 3 cut(s) 252, 679, 979
Lsp1109I GCAGC 3 cut(s) 588, 591, 594
LweI GCATC 3 cut(s) 31, 383, 752
MaeI CTAG 3 cut(s) 195, 387, 1130
MaeII ACGT 2 cut(s) 599, 1078
MaeIII GTNAC 3 cut(s) 3, 126, 350
MalI GATC 2 cut(s) 145, 810
MboI GATC 2 cut(s) 143, 808
MfeI CAATTG 1 cut(s) 46
MluCI AATT 7 cut(s) 46, 66, 748, 838, 956, 1050, 1118
MmeI TCCRAC 3 cut(s) 582, 778, 891
MnlI CCTC 9 cut(s) 527, 736, 783, 796, 907, 997, 1056, 1116, 1136
MroI TCCGGA 1 cut(s) 414
MseI TTAA 4 cut(s) 453, 522, 530, 779
MspI CCGG 4 cut(s) 72, 250, 415, 977
MspR9I CCNGG 5 cut(s) 73, 250, 381, 425, 788
MunI CAATTG 1 cut(s) 46
Mva1269I GAATGC 2 cut(s) 505, 625
MvaI CCWGG 3 cut(s) 381, 425, 788
MwoI GCNNNNNNNGC 2 cut(s) 573, 582
NciI CCSGG 2 cut(s) 73, 250
NdeII GATC 2 cut(s) 143, 808
NlaIII CATG 5 cut(s) 242, 491, 836, 869, 944
NlaIV GGNNCC 4 cut(s) 367, 828, 981, 1035
NmuCI GTSAC 1 cut(s) 126
NspI RCATGY 1 cut(s) 836
NspV TTCGAA 1 cut(s) 1160
PaeR7I CTCGAG 1 cut(s) 1140
PceI AGGCCT 1 cut(s) 739
PciI ACATGT 1 cut(s) 832
PciSI GCTCTTC 1 cut(s) 106
PctI GAATGC 2 cut(s) 505, 625
PfeI GAWTC 5 cut(s) 302, 512, 659, 683, 862
PfoI TCCNGGA 2 cut(s) 71, 248
PkrI GCNGC 3 cut(s) 578, 581, 584
PpuMI RGGWCCY 1 cut(s) 1034
PscI ACATGT 1 cut(s) 832
Psp5II RGGWCCY 1 cut(s) 1034
Psp6I CCWGG 3 cut(s) 379, 423, 786
PspGI CCWGG 3 cut(s) 379, 423, 786
PspN4I GGNNCC 4 cut(s) 367, 828, 981, 1035
PspPI GGNCC 7 cut(s) 32, 329, 366, 383, 421, 604, 1034
PspPPI RGGWCCY 1 cut(s) 1034
PstNI CAGNNNCTG 1 cut(s) 893
RsaI GTAC 3 cut(s) 245, 1102, 1128
RsaNI GTAC 3 cut(s) 244, 1101, 1127
SapI GCTCTTC 1 cut(s) 106
SaqAI TTAA 4 cut(s) 453, 522, 530, 779
SatI GCNGC 3 cut(s) 577, 580, 583
Sau3AI GATC 2 cut(s) 143, 808
Sau96I GGNCC 7 cut(s) 32, 329, 366, 383, 421, 604, 1034
ScrFI CCNGG 5 cut(s) 73, 250, 381, 425, 788
SfaNI GCATC 3 cut(s) 31, 383, 752
Sfr274I CTCGAG 1 cut(s) 1140
SfuI TTCGAA 1 cut(s) 1160
SinI GGWCC 5 cut(s) 32, 383, 421, 604, 1034
SlaI CTCGAG 1 cut(s) 1140
SmlI CTYRAG 2 cut(s) 905, 1140
SmoI CTYRAG 2 cut(s) 905, 1140
Sse9I AATT 7 cut(s) 46, 66, 748, 838, 956, 1050, 1118
SseBI AGGCCT 1 cut(s) 739
SsiI CCGC 1 cut(s) 1197
SspMI CTAG 3 cut(s) 195, 387, 1130
StuI AGGCCT 1 cut(s) 739
StyD4I CCNGG 5 cut(s) 71, 248, 379, 423, 786
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 3 cut(s) 508, 572, 1207
TaiI ACGT 2 cut(s) 602, 1081
TaqI TCGA 3 cut(s) 746, 1141, 1160
TaqII GACCGA 1 cut(s) 325
TasI AATT 7 cut(s) 46, 66, 748, 838, 956, 1050, 1118
TatI WGTACW 1 cut(s) 1100
TfiI GAWTC 5 cut(s) 302, 512, 659, 683, 862
Tru1I TTAA 4 cut(s) 453, 522, 530, 779
Tru9I TTAA 4 cut(s) 453, 522, 530, 779
TscAI CASTG 2 cut(s) 376, 577
TseFI GTSAC 1 cut(s) 126
TseI GCWGC 3 cut(s) 576, 579, 582
Tsp45I GTSAC 1 cut(s) 126
TspDTI ATGAA 3 cut(s) 5, 315, 1181
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 2 cut(s) 376, 577
VpaK11BI GGWCC 5 cut(s) 32, 383, 421, 604, 1034
XagI CCTNNNNNAGG 2 cut(s) 698, 733
XapI RAATTY 4 cut(s) 66, 748, 838, 956
XceI RCATGY 1 cut(s) 836
XhoI CTCGAG 1 cut(s) 1140
XspI CTAG 3 cut(s) 195, 387, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.