AT1G07380

Neutral/alkaline non-lysosomal ceramidase, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010845
Physical Location & Seq
Reverse (-)
2264634 .. 2269121
4488 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G07380.2

Sequence Viewer

Length: 2340 bp
ATGGAGCTATCTCTAGTCAGATTATGGAACACTTGTTTTCCTAGTTTCTTCTACATTTCGACTGTGCTTTTTCTGCTCACCGGTGGAGGAGTTTACTCTCACTCTGAATATTTGATTGGATTGGGAAGCTATGACATTACTGGCCCGGCTGCTGATGTTAATATGATGGGTTATGCTAACATGGAGCAAGTAGCATCTGGTATTCACTTCAGGCTCCGTGCTAGGACTTTCATTGTCTCTGAGCCCCAAGGGAAGCGGGTTGTGTTTGTTAACCTCGATGCTTGCATGGCTTCACAAATTGTAAAACTAAAAGTGATCGAGAGACTCAAGGCACGATATGGGGACTTATATACTGAACAGAATGTTGGCATCAGTGGTATTCATACCCATGCTGGTCCAGGGGGGTATCTTCAGTATGTGGTGTATATCGTAACATCACTTGGATTTGTTCGTCAGTCGTTTGATGCCCTTGTCGATGGAATTGAGAACAGTATCATACAAGCTCACGAAAATCTTCGTCCTGGATCAATTTTTCTCAATAATGGGGAACTCTTGGATGCTGGTGTAAACCGCAGTCCAAGTGCATATCTTAATAATCCTTCAAAAGAAAGGAGTAAACACAAGTACAACGTTGATAAGGAAATGACTCTCTTAAAGTTTGTGGATGATCAATGGGGTCCAGTAGGTAGTTTCAATTGGTTTGCTACCCATGGAACTTCCATGAGTCGTACAAACTCTTTGATCAGTGGGGACAACAAGGGCGCTGCATCACGATTTATGGAGGATTGGTATGAACAGAATACTGCTGAAAGATCTTACTCTGAAGAGTTTATTTCAGATGAGATACCTAGAAGAGTTTCAAGCCTAATCGAGAATCATCAGGACAGTCATCACGAGTTGCTAGAGCTTGCATCTTACTTTGAGTCACAACCTGGTAAGCCTGTAACTAGAATTTCAAGTTCTGCAAGGCGGGTGAGGAGTGCTCTGAGGAAGGCGGATAAGCCTGGCTTCGTTTCTGCTTTCTGTCAAACGAACTGTGGAGATGTTAGCCCAAATGTGCTTGGAGCCTTTTGCCTGGACACTGGTCTTCCTTGTGATTTCAATCACAGTACATGTGGTGGGAAAAATGAGATGTGCTATGGGCGTGGACCAGGCTATCCTGATGAATTTGAAAGTACACGTATAATTGGTGAGAGACAATTCAAGATGGCTTTGGAACTTTTCAACAAAGCATCTGAACAGCTACAAGGGAAGGTTGATTACCGCCATGTTTATGTTGACTTTTCACAGCTCAATGTGACACTTCCTAAAAAAGATGGGAAATCAGAAGTTGTAAAAACATGTCCTGCGGCAATGGGCTTTGCTTTCGCTGCTGGGACCACAGATGGACCAGGAGCATTTGATTTTACACAAGGAGATGATAAGGGGAACCCTTTCTGGAGATTGGTGAGAAATGTTCTTAAAACACCGGACAAGAAACAAATCGATTGTCACTATCCAAAACCTATCTTGCTTGACACTGGAGAAATGACAAAGCCATATGACTGGGCTCCGTCAATTCTTTCATTGCAAGTTCTACGTATTGGACAACTTTTCATTCTCAGCGTTCCTGGAGAATTTACAACTATGGCTGGAAGGCGTCTCCGCTATGCAGTGAAAACACAACTTAAAAACAGTGGTAATAAAGATTTGAGCGGTGAGATTCACGTTGTGATAGCTGGATTGGCTAATGGTTACTCACAGTATGTGACTACTTTTGAGGAGTATCAAGTGCAGAGATACGAGGGTGCATCTACGTTATATGGGCCACACACCCTCAGTGGCTACATTCAAGAGTTCAAGAAACTCTCCAAATCTCTCGTCCTTGATATGCCTGTTCAACCGGGGCCTCAACCGCCGGATCTCCTTGACAAGCAACTAAGCTTCCTCACACCAGTCATGATGGACACAACTCCTAGCGGAGACAGTTTCGGGGATGTTATTTCGGATGTTCCTAAAAACTTGTCTTTGAAGAGAGGAAACGGCCAGGTGACCGTTGTATTCCGGTCAGCCTGCCCTAGAAACGATCTACTGACAGAAGGGACGTTTACACTGGTGGAGAGACTAGAGCAGAAGGACAAGACTTGGACCCCGGTTTATGACGACGATGATCTATGTCTGAGGTTCAAGTGGTCGAGGCATAAAAAGCTGAGCTCCCGGAGTCAGGCCACTGTGGAATGGAGAATTCCCGAGTCTGCATCACCAGGGGTTTATAGGATCACCCATTTTGGTGCTGCAAAGAAGCTTTTTGGGTCAGTCCATCATTTCACAGGCTCTTCTAGTGCTTTCGTTGTAACATAA

Protein Analysis

779

Amino Acids

86.77

Weight (kDa)

7.27

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 38 - 612 4.8e-228 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 614 - 779 1.4e-54 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1695
AclI AACGTT 1 cut(s) 630
AclWI GGATC 3 cut(s) 532, 1908, 2264
AcoI YGGCCR 1 cut(s) 2023
AcsI RAATTY 4 cut(s) 951, 1166, 1616, 2223
AcuI CTGAAG 3 cut(s) 193, 395, 843
AcyI GRCGYC 1 cut(s) 1639
AdeI CACNNNGTG 1 cut(s) 1711
AfaI GTAC 4 cut(s) 626, 730, 1111, 1177
AfiI CCNNNNNNNGG 1 cut(s) 2203
AflIII ACRYGT 3 cut(s) 1112, 1178, 1340
AgeI ACCGGT 1 cut(s) 80
AjuI GAANNNNNNNTTGG 4 cut(s) 99, 131, 348, 380
Alw21I GWGCWC 2 cut(s) 985, 2195
Alw26I GTCTC 6 cut(s) 241, 316, 1189, 1646, 1956, 2095
AlwI GGATC 3 cut(s) 532, 1908, 2264
Ama87I CYCGRG 1 cut(s) 2228
AoxI GGCC 5 cut(s) 142, 1805, 1886, 2023, 2205
ApeKI GCWGC 4 cut(s) 149, 764, 1370, 2273
ApoI RAATTY 4 cut(s) 951, 1166, 1616, 2223
ArsI GACNNNNNNTTYG 4 cut(s) 1474, 1506, 1845, 1877
AsiGI ACCGGT 1 cut(s) 80
Asp700I GAANNNNTTC 3 cut(s) 513, 856, 1433
AspLEI GCGC 1 cut(s) 764
AspS9I GGNCC 9 cut(s) 143, 395, 677, 1148, 1377, 1388, 1805, 1886, 2127
AsuC2I CCSGG 4 cut(s) 146, 1884, 2132, 2197
AsuHPI GGTGA 8 cut(s) 70, 985, 1202, 1459, 1709, 2041, 2232, 2251
AvaI CYCGRG 1 cut(s) 2228
AvaII GGWCC 6 cut(s) 395, 677, 1148, 1377, 1388, 2127
BaeI ACNNNNGTAYC 2 cut(s) 1771, 1804
BanII GRGCYC 3 cut(s) 246, 1552, 2195
BarI GAAGNNNNNNTAC 2 cut(s) 1244, 1276
BauI CACGAG 1 cut(s) 893
BbsI GAAGAC 1 cut(s) 1079
Bbv12I GWGCWC 2 cut(s) 985, 2195
BbvI GCAGC 4 cut(s) 136, 751, 1357, 2260
BccI CCATC 7 cut(s) 160, 470, 1201, 1310, 1379, 1936, 2307
BceAI ACGGC 1 cut(s) 2038
BclI TGATCA 2 cut(s) 667, 741
BcnI CCSGG 4 cut(s) 146, 1884, 2132, 2197
BcoDI GTCTC 6 cut(s) 241, 316, 1189, 1646, 1956, 2095
BfoI RGCGCY 1 cut(s) 765
BglII AGATCT 1 cut(s) 812
BisI GCNGC 5 cut(s) 150, 765, 1350, 1371, 2274
BlpI GCTNAGC 1 cut(s) 2189
BlsI GCNGC 5 cut(s) 151, 766, 1351, 1372, 2275
Bme18I GGWCC 6 cut(s) 395, 677, 1148, 1377, 1388, 2127
BmeT110I CYCGRG 1 cut(s) 2228
BmgT120I GGNCC 9 cut(s) 143, 395, 677, 1148, 1377, 1388, 1805, 1886, 2127
BmiI GGNNCC 8 cut(s) 215, 678, 1066, 1378, 1430, 1551, 1887, 2129
BmrI ACTGGG 1 cut(s) 1555
BmuI ACTGGG 1 cut(s) 1555
BoxI GACNNNNGTC 1 cut(s) 1083
BpiI GAAGAC 1 cut(s) 1079
BplI GAGNNNNNCTC 2 cut(s) 967, 999
BpmI CTGGAG 3 cut(s) 1459, 1542, 1632
Bpu1102I GCTNAGC 1 cut(s) 2189
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 4 cut(s) 146, 1884, 2132, 2197
Bsa29I ATCGAT 1 cut(s) 1485
BsaAI YACGTR 2 cut(s) 1181, 1580
BsaBI GATNNNNATC 1 cut(s) 1101
BsaHI GRCGYC 1 cut(s) 1639
BsaJI CCNNGG 6 cut(s) 247, 398, 709, 1883, 2130, 2243
BsaWI WCCGGW 3 cut(s) 80, 1468, 2043
BsaXI ACNNNNNCTCC 2 cut(s) 773, 803
Bsc4I CCNNNNNNNGG 1 cut(s) 2203
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 7 cut(s) 145, 680, 1087, 1525, 1550, 1934, 2097
Bse3DI GCAATG 2 cut(s) 1359, 1565
Bse8I GATNNNNATC 1 cut(s) 1101
BseCI ATCGAT 1 cut(s) 1485
BseDI CCNNGG 6 cut(s) 247, 398, 709, 1883, 2130, 2243
BseGI GGATG 4 cut(s) 562, 670, 1981, 1993
BseJI GATNNNNATC 1 cut(s) 1101
BseLI CCNNNNNNNGG 1 cut(s) 2203
BseMI GCAATG 2 cut(s) 1359, 1565
BseMII CTCAG 6 cut(s) 231, 977, 1615, 1831, 2150, 2180
BseNI ACTGG 7 cut(s) 145, 680, 1087, 1525, 1550, 1934, 2097
BseRI GAGGAG 3 cut(s) 102, 991, 1775
BseXI GCAGC 4 cut(s) 136, 751, 1357, 2260
BseYI CCCAGC 1 cut(s) 1373
BsgI GTGCAG 1 cut(s) 1793
BshFI GGCC 5 cut(s) 144, 1807, 1888, 2025, 2207
BshTI ACCGGT 1 cut(s) 80
BshVI ATCGAT 1 cut(s) 1485
BsiHKAI GWGCWC 2 cut(s) 985, 2195
BsiHKCI CYCGRG 1 cut(s) 2228
BsiSI CCGG 8 cut(s) 81, 146, 1469, 1883, 1898, 2044, 2132, 2197
BslFI GGGAC 4 cut(s) 356, 764, 1390, 2095
BslI CCNNNNNNNGG 1 cut(s) 2203
BsmAI GTCTC 6 cut(s) 241, 316, 1189, 1646, 1956, 2095
BsmBI CGTCTC 1 cut(s) 1646
BsmFI GGGAC 4 cut(s) 356, 764, 1390, 2095
BsnI GGCC 5 cut(s) 144, 1807, 1888, 2025, 2207
BsoBI CYCGRG 1 cut(s) 2228
Bsp1286I GDGCHC 4 cut(s) 246, 985, 1552, 2195
Bsp143I GATC 9 cut(s) 315, 524, 667, 741, 812, 1900, 2065, 2149, 2256
Bsp1720I GCTNAGC 1 cut(s) 2189
Bsp19I CCATGG 1 cut(s) 709
BspANI GGCC 5 cut(s) 144, 1807, 1888, 2025, 2207
BspCNI CTCAG 6 cut(s) 232, 978, 1614, 1830, 2151, 2181
BspDI ATCGAT 1 cut(s) 1485
BspHI TCATGA 1 cut(s) 1938
BspLI GGNNCC 8 cut(s) 215, 678, 1066, 1378, 1430, 1551, 1887, 2129
BspPI GGATC 3 cut(s) 532, 1908, 2264
BspQI GCTCTTC 1 cut(s) 2320
BsrBI CCGCTC 1 cut(s) 1695
BsrDI GCAATG 2 cut(s) 1359, 1565
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 7 cut(s) 145, 680, 1087, 1525, 1550, 1934, 2097
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 6 cut(s) 247, 398, 709, 1883, 2130, 2243
BssMI GATC 9 cut(s) 315, 524, 667, 741, 812, 1900, 2065, 2149, 2256
BssNI GRCGYC 1 cut(s) 1639
BssSI CACGAG 1 cut(s) 893
BssT1I CCWWGG 2 cut(s) 247, 709
Bst2BI CACGAG 1 cut(s) 893
Bst6I CTCTTC 4 cut(s) 819, 847, 2006, 2320
BstACI GRCGYC 1 cut(s) 1639
BstBAI YACGTR 2 cut(s) 1181, 1580
BstC8I GCNNGC 3 cut(s) 283, 909, 2053
BstDEI CTNAG 7 cut(s) 240, 986, 1601, 1817, 1919, 2159, 2189
BstDSI CCRYGG 1 cut(s) 709
BstEII GGTNACC 1 cut(s) 2029
BstF5I GGATG 4 cut(s) 562, 670, 1981, 1993
BstH2I RGCGCY 1 cut(s) 765
BstHHI GCGC 1 cut(s) 764
BstKTI GATC 9 cut(s) 318, 527, 670, 744, 815, 1903, 2068, 2152, 2259
BstMAI GTCTC 6 cut(s) 241, 316, 1189, 1646, 1956, 2095
BstMBI GATC 9 cut(s) 315, 524, 667, 741, 812, 1900, 2065, 2149, 2256
BstMWI GCNNNNNNNGC 6 cut(s) 73, 287, 1370, 1724, 1894, 2185
BstNSI RCATGY 2 cut(s) 1116, 1344
BstPAI GACNNNNGTC 1 cut(s) 1083
BstPI GGTNACC 1 cut(s) 2029
BstSNI TACGTA 1 cut(s) 1580
BstV1I GCAGC 4 cut(s) 136, 751, 1357, 2260
BstV2I GAAGAC 1 cut(s) 1079
BstX2I RGATCY 2 cut(s) 812, 1900
BstXI CCANNNNNNTGG 1 cut(s) 1545
BstYI RGATCY 2 cut(s) 812, 1900
Bsu15I ATCGAT 1 cut(s) 1485
BsuRI GGCC 5 cut(s) 144, 1807, 1888, 2025, 2207
BsuTUI ATCGAT 1 cut(s) 1485
BtgI CCRYGG 1 cut(s) 709
BtsCI GGATG 4 cut(s) 562, 670, 1981, 1993
BtsI GCAGTG 1 cut(s) 1659
BtsIMutI CAGTG 9 cut(s) 379, 751, 1080, 1518, 1659, 1681, 1825, 2090, 2208
Cac8I GCNNGC 3 cut(s) 283, 909, 2053
CciI TCATGA 1 cut(s) 1938
CfoI GCGC 1 cut(s) 764
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 9 cut(s) 143, 395, 677, 1148, 1377, 1388, 1805, 1886, 2127
ClaI ATCGAT 1 cut(s) 1485
CseI GACGC 1 cut(s) 1628
CsiI ACCWGGT 1 cut(s) 931
Csp6I GTAC 4 cut(s) 625, 729, 1110, 1176
CspAI ACCGGT 1 cut(s) 80
CviAII CATG 9 cut(s) 181, 286, 389, 710, 721, 1113, 1268, 1341, 1939
CviQI GTAC 4 cut(s) 625, 729, 1110, 1176
DdeI CTNAG 7 cut(s) 240, 986, 1601, 1817, 1919, 2159, 2189
DpnI GATC 9 cut(s) 317, 526, 669, 743, 814, 1902, 2067, 2151, 2258
DpnII GATC 9 cut(s) 315, 524, 667, 741, 812, 1900, 2065, 2149, 2256
DraIII CACNNNGTG 1 cut(s) 1711
EaeI YGGCCR 1 cut(s) 2023
Eam1104I CTCTTC 4 cut(s) 819, 847, 2006, 2320
EarI CTCTTC 4 cut(s) 819, 847, 2006, 2320
EciI GGCGGA 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 2193
Eco105I TACGTA 1 cut(s) 1580
Eco130I CCWWGG 2 cut(s) 247, 709
Eco24I GRGCYC 3 cut(s) 246, 1552, 2195
Eco47I GGWCC 6 cut(s) 395, 677, 1148, 1377, 1388, 2127
Eco53kI GAGCTC 1 cut(s) 2193
Eco57I CTGAAG 3 cut(s) 193, 395, 843
Eco88I CYCGRG 1 cut(s) 2228
Eco91I GGTNACC 1 cut(s) 2029
EcoICRI GAGCTC 1 cut(s) 2193
EcoO109I RGGNCCY 1 cut(s) 1886
EcoO65I GGTNACC 1 cut(s) 2029
EcoRI GAATTC 1 cut(s) 2223
EcoT14I CCWWGG 2 cut(s) 247, 709
EcoT38I GRGCYC 3 cut(s) 246, 1552, 2195
ErhI CCWWGG 2 cut(s) 247, 709
Esp3I CGTCTC 1 cut(s) 1646
FaeI CATG 9 cut(s) 184, 289, 392, 713, 724, 1116, 1271, 1344, 1942
FalI AAGNNNNNCTT 2 cut(s) 992, 1024
FaqI GGGAC 4 cut(s) 356, 764, 1390, 2095
FatI CATG 9 cut(s) 180, 285, 388, 709, 720, 1112, 1267, 1340, 1938
FauI CCCGC 2 cut(s) 249, 963
FauNDI CATATG 1 cut(s) 1540
FbaI TGATCA 2 cut(s) 667, 741
Fnu4HI GCNGC 5 cut(s) 150, 765, 1350, 1371, 2274
FokI GGATG 4 cut(s) 569, 677, 1988, 2000
FriOI GRGCYC 3 cut(s) 246, 1552, 2195
Fsp4HI GCNGC 5 cut(s) 150, 765, 1350, 1371, 2274
GlaI GCGC 1 cut(s) 763
GluI GCNGC 5 cut(s) 150, 765, 1350, 1371, 2274
GsaI CCCAGC 1 cut(s) 1377
GsuI CTGGAG 3 cut(s) 1459, 1542, 1632
HaeII RGCGCY 1 cut(s) 765
HaeIII GGCC 5 cut(s) 144, 1807, 1888, 2025, 2207
HapII CCGG 8 cut(s) 81, 146, 1469, 1883, 1898, 2044, 2132, 2197
HgaI GACGC 1 cut(s) 1628
HhaI GCGC 1 cut(s) 764
Hin1I GRCGYC 1 cut(s) 1639
Hin1II CATG 9 cut(s) 184, 289, 392, 713, 724, 1116, 1271, 1344, 1942
Hin6I GCGC 1 cut(s) 762
HinP1I GCGC 1 cut(s) 762
HincII GTYRAC 2 cut(s) 271, 1279
HindII GTYRAC 2 cut(s) 271, 1279
HindIII AAGCTT 2 cut(s) 1921, 2282
HinfI GANTC 8 cut(s) 324, 646, 724, 874, 923, 1702, 2200, 2231
HpaI GTTAAC 1 cut(s) 271
HpaII CCGG 8 cut(s) 81, 146, 1469, 1883, 1898, 2044, 2132, 2197
HphI GGTGA 8 cut(s) 70, 985, 1202, 1459, 1709, 2041, 2232, 2251
Hpy166II GTNNAC 8 cut(s) 94, 271, 568, 617, 1148, 1178, 1279, 2088
Hpy8I GTNNAC 8 cut(s) 94, 271, 568, 617, 1148, 1178, 1279, 2088
Hpy99I CGWCG 1 cut(s) 2147
HpyAV CCTTC 6 cut(s) 609, 985, 1246, 1629, 2072, 2107
HpyCH4IV ACGT 6 cut(s) 630, 1180, 1579, 1707, 1796, 2084
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 287, 1370, 1724, 1894, 2185
HpyF3I CTNAG 7 cut(s) 240, 986, 1601, 1817, 1919, 2159, 2189
HpySE526I ACGT 6 cut(s) 630, 1180, 1579, 1707, 1796, 2084
Hsp92I GRCGYC 1 cut(s) 1639
Hsp92II CATG 9 cut(s) 184, 289, 392, 713, 724, 1116, 1271, 1344, 1942
HspAI GCGC 1 cut(s) 762
Ksp22I TGATCA 2 cut(s) 667, 741
KspAI GTTAAC 1 cut(s) 271
Kzo9I GATC 9 cut(s) 315, 524, 667, 741, 812, 1900, 2065, 2149, 2256
LguI GCTCTTC 1 cut(s) 2320
LmnI GCTCC 7 cut(s) 4, 184, 219, 1064, 1394, 1555, 2198
Lsp1109I GCAGC 4 cut(s) 136, 751, 1357, 2260
MabI ACCWGGT 1 cut(s) 931
MaeII ACGT 6 cut(s) 630, 1180, 1579, 1707, 1796, 2084
MaeIII GTNAC 9 cut(s) 430, 924, 943, 1297, 1490, 1733, 1747, 2029, 2332
MalI GATC 9 cut(s) 317, 526, 669, 743, 814, 1902, 2067, 2151, 2258
MbiI CCGCTC 1 cut(s) 1695
MboI GATC 9 cut(s) 315, 524, 667, 741, 812, 1900, 2065, 2149, 2256
MboII GAAGA 8 cut(s) 40, 401, 506, 836, 864, 1079, 2023, 2307
MfeI CAATTG 1 cut(s) 694
MflI RGATCY 2 cut(s) 812, 1900
MhlI GDGCHC 4 cut(s) 246, 985, 1552, 2195
MlyI GAGTC 6 cut(s) 318, 640, 733, 932, 2209, 2240
MroXI GAANNNNTTC 3 cut(s) 513, 856, 1433
MseI TTAA 6 cut(s) 159, 270, 591, 653, 1461, 1668
MslI CAYNNNNRTG 3 cut(s) 387, 1272, 2268
MspI CCGG 8 cut(s) 81, 146, 1469, 1883, 1898, 2044, 2132, 2197
MunI CAATTG 1 cut(s) 694
MwoI GCNNNNNNNGC 6 cut(s) 73, 287, 1370, 1724, 1894, 2185
NciI CCSGG 4 cut(s) 146, 1884, 2132, 2197
NcoI CCATGG 1 cut(s) 709
NdeI CATATG 1 cut(s) 1540
NdeII GATC 9 cut(s) 315, 524, 667, 741, 812, 1900, 2065, 2149, 2256
NlaIII CATG 9 cut(s) 184, 289, 392, 713, 724, 1116, 1271, 1344, 1942
NlaIV GGNNCC 8 cut(s) 215, 678, 1066, 1378, 1430, 1551, 1887, 2129
NmuCI GTSAC 5 cut(s) 924, 1297, 1490, 1747, 2029
NspI RCATGY 2 cut(s) 1116, 1344
PagI TCATGA 1 cut(s) 1938
PciI ACATGT 2 cut(s) 1112, 1340
PciSI GCTCTTC 1 cut(s) 2320
PdmI GAANNNNTTC 3 cut(s) 513, 856, 1433
PfeI GAWTC 2 cut(s) 874, 1702
PfoI TCCNGGA 3 cut(s) 520, 1609, 2195
PinAI ACCGGT 1 cut(s) 80
PkrI GCNGC 5 cut(s) 151, 766, 1351, 1372, 2275
PleI GAGTC 6 cut(s) 318, 640, 732, 931, 2208, 2239
PpsI GAGTC 6 cut(s) 318, 640, 732, 931, 2208, 2239
Ppu21I YACGTR 2 cut(s) 1181, 1580
PscI ACATGT 2 cut(s) 1112, 1340
PshAI GACNNNNGTC 1 cut(s) 1083
Psp124BI GAGCTC 1 cut(s) 2195
Psp1406I AACGTT 1 cut(s) 630
PspEI GGTNACC 1 cut(s) 2029
PspFI CCCAGC 1 cut(s) 1373
PspN4I GGNNCC 8 cut(s) 215, 678, 1066, 1378, 1430, 1551, 1887, 2129
PspPI GGNCC 9 cut(s) 143, 395, 677, 1148, 1377, 1388, 1805, 1886, 2127
PsuI RGATCY 2 cut(s) 812, 1900
RsaI GTAC 4 cut(s) 626, 730, 1111, 1177
RsaNI GTAC 4 cut(s) 625, 729, 1110, 1176
RseI CAYNNNNRTG 3 cut(s) 387, 1272, 2268
SacI GAGCTC 1 cut(s) 2195
SapI GCTCTTC 1 cut(s) 2320
SaqAI TTAA 6 cut(s) 159, 270, 591, 653, 1461, 1668
SatI GCNGC 5 cut(s) 150, 765, 1350, 1371, 2274
Sau3AI GATC 9 cut(s) 315, 524, 667, 741, 812, 1900, 2065, 2149, 2256
Sau96I GGNCC 9 cut(s) 143, 395, 677, 1148, 1377, 1388, 1805, 1886, 2127
SchI GAGTC 6 cut(s) 318, 640, 733, 932, 2209, 2240
SduI GDGCHC 4 cut(s) 246, 985, 1552, 2195
SexAI ACCWGGT 1 cut(s) 931
SgrAI CRCCGGYG 1 cut(s) 80
SinI GGWCC 6 cut(s) 395, 677, 1148, 1377, 1388, 2127
SmiMI CAYNNNNRTG 3 cut(s) 387, 1272, 2268
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SnaBI TACGTA 1 cut(s) 1580
SspI AATATT 1 cut(s) 110
SstI GAGCTC 1 cut(s) 2195
StyI CCWWGG 2 cut(s) 247, 709
TaiI ACGT 6 cut(s) 633, 1183, 1582, 1710, 1799, 2087
TaqI TCGA 7 cut(s) 59, 276, 318, 474, 870, 1485, 2174
TatI WGTACW 3 cut(s) 624, 1109, 1175
TauI GCSGC 1 cut(s) 1352
TfiI GAWTC 2 cut(s) 874, 1702
Tru1I TTAA 6 cut(s) 159, 270, 591, 653, 1461, 1668
Tru9I TTAA 6 cut(s) 159, 270, 591, 653, 1461, 1668
TscAI CASTG 9 cut(s) 379, 751, 1087, 1525, 1659, 1681, 1825, 2097, 2215
TseFI GTSAC 5 cut(s) 924, 1297, 1490, 1747, 2029
TseI GCWGC 4 cut(s) 149, 764, 1370, 2273
Tsp45I GTSAC 5 cut(s) 924, 1297, 1490, 1747, 2029
TspDTI ATGAA 6 cut(s) 220, 371, 807, 1179, 1554, 1585
TspGWI ACGGA 2 cut(s) 206, 1542
TspRI CASTG 9 cut(s) 379, 751, 1087, 1525, 1659, 1681, 1825, 2097, 2215
VpaK11BI GGWCC 6 cut(s) 395, 677, 1148, 1377, 1388, 2127
XapI RAATTY 4 cut(s) 951, 1166, 1616, 2223
XceI RCATGY 2 cut(s) 1116, 1344
XmnI GAANNNNTTC 3 cut(s) 513, 856, 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.