Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0010845

Physical Location & Seq

Genomic Coordinates Forward (+)

76612403 .. 76617870

Gene Structure 5468 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG445600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGACTCTTCTGAAGTTTGTCGATAATCAATGGGGTCCAGTGGGTAGCTTCAATTGGTTTGCAACTCATGGAACTTCTATGAGTCGGACAAACTCATTAATTAGTGGGGATAACAAGGGTGCTGCTGCACGATTTATGGAAGACTGGTTTGAGGAGAATGGTGGTAGAAATGCATATTCTGATGATATTGATGCTGCCGATATACCGCGAAGAGTGTCAAACATAGTTTCTGACCATCATGATAATCACCATGAGTTATTGGAGCTTGCTGCATCCTTTCAGTCTCCTCCTGGTAAGCCAGCTACCAGGTCCTTGAGTGTTGCAAGGCGTGTCAGGGGTGTACTAAGGCAGGCTAACAAGCCTAGATTTGTATCTGCCTTTTGTCAATCAAACTGTGGTGATGTGAGCCCAAATGTTCTAGGAGCTTTCTGCACAGATACTGGGCTACCTTGTGATTTCAATCACAGCACCTGTGGTGGGAAGAATGAGTTGTGCTATGGCCGAGGACCGGGTTACCCAGATGAATTTGAGAGTACGCGTATTATTGGTGAGAGGCAACTCAGAAAAGCGGTGGATCTTTTCAACAAAGCATCTGAACAGTTGACTGGGAAGGTTGAATATCGCCACACTTACATCGACTTCTCCCAACTTGAAGTAACACTTCCCAAGAAGGGGGGAGGATCTGAGGTGGTAAAGACATGTCCTGCTGCAATGGGGTTTGGATTTGCTGCTGGAACTACTGACGGACCTGGAGCTTTTGATTTCAAGCAAGGGGATGATAAGGGAAATCCTTTCTGGAAATTGGTGCGCAATGTACTCAAAACACCAGGCCAGGAACAAATTGATTGTCAGAGTCCAAAGCCAATCTTGCTTGATACTGGTGAAATGAAGCAACCATATGACTGGGCGCCTGCAATACTTCCAATTCAGATCTTCCGGATAGGGCAGCTTGTCATTCTCAGTGTACCTGGAGAATTTACAACAATGGCTGGTAGGCGGCTCCGTGATGCTGTGAAAACGGTACTGACTAGTGGTGGTCGTGGTGGAAATATTCATGTTGTGCTAGCAGGATTGACTAATACATATTCTCAGTATATCACGACCTTTGAAGAGTATGAGGTGCAGAGATATGAGGGTGCATCCACTCTCTATGGACCACACACGCTAAGCGCCTACATTCAGGAGTTCAAGAAGCTTGCTACAGCTCTCATCAGTAACCAACCTGTCGCATCAGGTCCACAACCCCCAGATCTCCTTGATAAGCAGATAAGCTTGCTTACTCCAGTTGTTATGGATGCAACCCCTGTTGGTGTCAGTTTTGGGGATTGCAGTTCGGATGTGCCTCAAAACTCCACCTTTAAGAGAGGTCATGACATGGTAACAGTTACCTTCTGGTCGGCTTGTCCTCGGAATGACCTATTGACGGAAGGTACATTTGCCCTTGTGGAGATTCTCCATGGAAAGGATACTTGGGTTCCAGCTTACGATGACGATGATTTCTGTCTGCGCTTTAAGTGGTCAAGACCTTCCAAACTCAGTACTAGAAGTCGGGCGACCATGGAATGGAGAATTCCTCAGTCCGCAACTCCCGGCGTATACAGAATAAGGCATTTTGGTGCCTCTAAGAGCCTGGTTGGATCGATTCGCCACTTTACGGGTTCATCTAGTGCTTTTGTGGTAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0006950 GO:0006979 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0019725 GO:0031090 GO:0033554 GO:0034599 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0055088 GO:0065007 GO:0065008 GO:0070887 GO:0090156 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00600 ko01100 ko04071 map00600 map01100 map04071

Pfam Domains

Protein Families

Ceramidase_alk Ceramidse_alk_C

Protein Analysis

561

Amino Acids

61.61

Weight (kDa)

7.19

Isoelectric Point (pI)

36.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ceramidase_alk	PF04734	1 - 394	3e-141	Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C	PF17048	396 - 560	2e-54	Neutral/alkaline non-lysosomal ceramidase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0010845)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07380 AT1G07380 AT5G58980
fragaria_vesca	FvH4_3g37680
malus_domestica	MD03G1079000.v1.1
prunus_persica	Prupe.6G063600_v2.0.a1
pyrus_communis	pycom03g06300
rosa_chinensis	RchiOBHm_Chr5g0067951
rosa_laevigata	RLG00000035946
rosa_multiflora	Rmu_sc0000435.1_g000039
rosa_roxburghii	Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa	Rorug05G0386600 Rorug05G0386700
rosa_samantha	Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana	Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	809
AccB1I	GGYRCC	2 cut(s)	907, 1616
AccB7I	CCANNNNNTGG	1 cut(s)	1563
AccI	GTMKAC	1 cut(s)	1596
AccII	CGCG	2 cut(s)	208, 538
AccIII	TCCGGA	1 cut(s)	936
AciI	CCGC	4 cut(s)	206, 569, 997, 1581
AclWI	GGATC	3 cut(s)	582, 688, 1645
AcoI	YGGCCR	1 cut(s)	499
AcsI	RAATTY	3 cut(s)	524, 974, 1569
AcuI	CTGAAG	1 cut(s)	32
AcyI	GRCGYC	1 cut(s)	908
AfaI	GTAC	7 cut(s)	342, 535, 816, 966, 1023, 1432, 1540
AfiI	CCNNNNNNNGG	8 cut(s)	477, 508, 671, 672, 1423, 1462, 1563, 1654
AflIII	ACRYGT	2 cut(s)	536, 698
AgsI	TTSAA	8 cut(s)	52, 460, 583, 617, 653, 766, 1109, 1189
AhlI	ACTAGT	1 cut(s)	1028
AjnI	CCWGG	7 cut(s)	289, 305, 748, 826, 831, 967, 1629
AjuI	GAANNNNNNNTTGG	2 cut(s)	1453, 1485
Alw26I	GTCTC	1 cut(s)	288
AlwI	GGATC	3 cut(s)	582, 688, 1645
AlwNI	CAGNNNCTG	2 cut(s)	440, 471
Aor13HI	TCCGGA	1 cut(s)	936
AoxI	GGCC	2 cut(s)	499, 829
ApeKI	GCWGC	7 cut(s)	122, 125, 194, 269, 707, 728, 946
ApoI	RAATTY	3 cut(s)	524, 974, 1569
ArsI	GACNNNNNNTTYG	2 cut(s)	832, 864
AseI	ATTAAT	1 cut(s)	98
AspLEI	GCGC	4 cut(s)	810, 910, 1172, 1509
AspS9I	GGNCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
AsuC2I	CCSGG	2 cut(s)	510, 1590
AsuHPI	GGTGA	4 cut(s)	239, 410, 560, 893
AsuNHI	GCTAGC	1 cut(s)	1063
AvaII	GGWCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
BaeI	ACNNNNGTAYC	2 cut(s)	1458, 1491
BanI	GGYRCC	2 cut(s)	907, 1616
BanII	GRGCYC	1 cut(s)	410
BbsI	GAAGAC	1 cut(s)	147
BbvI	GCAGC	7 cut(s)	109, 112, 181, 256, 694, 715, 958
BccI	CCATC	1 cut(s)	243
BciT130I	CCWGG	7 cut(s)	291, 307, 750, 828, 833, 969, 1631
BciVI	GTATCC	1 cut(s)	1459
BcnI	CCSGG	2 cut(s)	510, 1590
BcoDI	GTCTC	1 cut(s)	288
BcuI	ACTAGT	1 cut(s)	1028
BfaI	CTAG	6 cut(s)	363, 419, 1029, 1064, 1542, 1665
BfmI	CTRYAG	1 cut(s)	1200
BfoI	RGCGCY	2 cut(s)	911, 1173
BfuI	GTATCC	1 cut(s)	1459
BglII	AGATCT	2 cut(s)	930, 1249
BisI	GCNGC	8 cut(s)	123, 126, 195, 270, 708, 729, 947, 998
BlpI	GCTNAGC	1 cut(s)	1166
BlsI	GCNGC	8 cut(s)	124, 127, 196, 271, 709, 730, 948, 999
BmcAI	AGTACT	1 cut(s)	1540
Bme1390I	CCNGG	9 cut(s)	291, 307, 510, 750, 828, 833, 969, 1590, 1631
Bme18I	GGWCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
BmgT120I	GGNCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
BmiI	GGNNCC	5 cut(s)	36, 909, 1001, 1476, 1618
BmrFI	CCNGG	9 cut(s)	291, 307, 510, 750, 828, 833, 969, 1590, 1631
BmrI	ACTGGG	3 cut(s)	450, 615, 913
BmsI	GCATC	7 cut(s)	181, 281, 599, 997, 1148, 1238, 1285
BmtI	GCTAGC	1 cut(s)	1067
BmuI	ACTGGG	3 cut(s)	450, 615, 913
BpiI	GAAGAC	1 cut(s)	147
BplI	GAGNNNNNCTC	2 cut(s)	1558, 1590
BpmI	CTGGAG	3 cut(s)	771, 990, 1266
Bpu1102I	GCTNAGC	1 cut(s)	1166
BpuEI	CTTGAG	1 cut(s)	334
BpuMI	CCSGG	2 cut(s)	510, 1590
Bsa29I	ATCGAT	1 cut(s)	1640
BsaBI	GATNNNNATC	2 cut(s)	370, 459
BsaHI	GRCGYC	1 cut(s)	908
BsaJI	CCNNGG	4 cut(s)	502, 1406, 1456, 1557
BsaWI	WCCGGW	1 cut(s)	936
Bsc4I	CCNNNNNNNGG	8 cut(s)	477, 508, 671, 672, 1423, 1462, 1563, 1654
Bse1I	ACTGG	7 cut(s)	38, 149, 445, 610, 883, 908, 1283
Bse3DI	GCAATG	2 cut(s)	717, 817
Bse8I	GATNNNNATC	2 cut(s)	370, 459
BseAI	TCCGGA	1 cut(s)	936
BseBI	CCWGG	7 cut(s)	291, 307, 750, 828, 833, 969, 1631
BseCI	ATCGAT	1 cut(s)	1640
BseDI	CCNNGG	4 cut(s)	502, 1406, 1456, 1557
BseGI	GGATG	5 cut(s)	272, 781, 1139, 1300, 1342
BseJI	GATNNNNATC	2 cut(s)	370, 459
BseLI	CCNNNNNNNGG	8 cut(s)	477, 508, 671, 672, 1423, 1462, 1563, 1654
BseMI	GCAATG	2 cut(s)	717, 817
BseMII	CTCAG	6 cut(s)	574, 675, 973, 1103, 1549, 1589
BseNI	ACTGG	7 cut(s)	38, 149, 445, 610, 883, 908, 1283
BseRI	GAGGAG	2 cut(s)	167, 276
BseXI	GCAGC	7 cut(s)	109, 112, 181, 256, 694, 715, 958
BsgI	GTGCAG	3 cut(s)	111, 415, 1142
Bsh1236I	CGCG	2 cut(s)	208, 538
BshFI	GGCC	2 cut(s)	501, 831
BshNI	GGYRCC	2 cut(s)	907, 1616
BshVI	ATCGAT	1 cut(s)	1640
BsiSI	CCGG	3 cut(s)	509, 937, 1590
BslI	CCNNNNNNNGG	8 cut(s)	477, 508, 671, 672, 1423, 1462, 1563, 1654
BsmAI	GTCTC	1 cut(s)	288
BsnI	GGCC	2 cut(s)	501, 831
Bsp1286I	GDGCHC	1 cut(s)	410
Bsp13I	TCCGGA	1 cut(s)	936
Bsp143I	GATC	5 cut(s)	574, 680, 930, 1249, 1637
Bsp1720I	GCTNAGC	1 cut(s)	1166
Bsp19I	CCATGG	2 cut(s)	1456, 1557
BspACI	CCGC	4 cut(s)	206, 569, 997, 1581
BspANI	GGCC	2 cut(s)	501, 831
BspCNI	CTCAG	6 cut(s)	573, 676, 972, 1102, 1548, 1588
BspDI	ATCGAT	1 cut(s)	1640
BspEI	TCCGGA	1 cut(s)	936
BspFNI	CGCG	2 cut(s)	208, 538
BspHI	TCATGA	2 cut(s)	238, 1369
BspLI	GGNNCC	5 cut(s)	36, 909, 1001, 1476, 1618
BspOI	GCTAGC	1 cut(s)	1067
BspPI	GGATC	3 cut(s)	582, 688, 1645
BspT107I	GGYRCC	2 cut(s)	907, 1616
BsrDI	GCAATG	2 cut(s)	717, 817
BsrI	ACTGG	7 cut(s)	38, 149, 445, 610, 883, 908, 1283
BssECI	CCNNGG	4 cut(s)	502, 1406, 1456, 1557
BssMI	GATC	5 cut(s)	574, 680, 930, 1249, 1637
BssNAI	GTATAC	1 cut(s)	1597
BssNI	GRCGYC	1 cut(s)	908
BssT1I	CCWWGG	2 cut(s)	1456, 1557
Bst1107I	GTATAC	1 cut(s)	1597
Bst2UI	CCWGG	7 cut(s)	291, 307, 750, 828, 833, 969, 1631
Bst4CI	ACNGT	4 cut(s)	395, 600, 1021, 1384
Bst6I	CTCTTC	3 cut(s)	12, 205, 1104
BstACI	GRCGYC	1 cut(s)	908
BstC8I	GCNNGC	7 cut(s)	267, 300, 351, 912, 1065, 1197, 1274
BstDEI	CTNAG	9 cut(s)	344, 560, 684, 959, 1089, 1166, 1535, 1575, 1623
BstDSI	CCRYGG	2 cut(s)	1456, 1557
BstEII	GGTNACC	1 cut(s)	512
BstF5I	GGATG	5 cut(s)	272, 781, 1139, 1300, 1342
BstFNI	CGCG	2 cut(s)	208, 538
BstH2I	RGCGCY	2 cut(s)	911, 1173
BstHHI	GCGC	4 cut(s)	810, 910, 1172, 1509
BstKTI	GATC	5 cut(s)	577, 683, 933, 1252, 1640
BstMAI	GTCTC	1 cut(s)	288
BstMBI	GATC	5 cut(s)	574, 680, 930, 1249, 1637
BstMWI	GCNNNNNNNGC	1 cut(s)	868
BstNI	CCWGG	7 cut(s)	291, 307, 750, 828, 833, 969, 1631
BstNSI	RCATGY	1 cut(s)	702
BstPI	GGTNACC	1 cut(s)	512
BstSCI	CCNGG	9 cut(s)	289, 305, 508, 748, 826, 831, 967, 1588, 1629
BstSFI	CTRYAG	1 cut(s)	1200
BstUI	CGCG	2 cut(s)	208, 538
BstV1I	GCAGC	7 cut(s)	109, 112, 181, 256, 694, 715, 958
BstV2I	GAAGAC	1 cut(s)	147
BstX2I	RGATCY	4 cut(s)	574, 680, 930, 1249
BstXI	CCANNNNNNTGG	1 cut(s)	903
BstYI	RGATCY	4 cut(s)	574, 680, 930, 1249
BstZ17I	GTATAC	1 cut(s)	1597
Bsu15I	ATCGAT	1 cut(s)	1640
BsuI	GTATCC	1 cut(s)	1459
BsuRI	GGCC	2 cut(s)	501, 831
BsuTUI	ATCGAT	1 cut(s)	1640
BtgI	CCRYGG	2 cut(s)	1456, 1557
BtsCI	GGATG	5 cut(s)	272, 781, 1139, 1300, 1342
BtsIMutI	CAGTG	2 cut(s)	45, 967
Cac8I	GCNNGC	7 cut(s)	267, 300, 351, 912, 1065, 1197, 1274
CaiI	CAGNNNCTG	2 cut(s)	440, 471
CciI	TCATGA	2 cut(s)	238, 1369
CfoI	GCGC	4 cut(s)	810, 910, 1172, 1509
Cfr13I	GGNCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
ClaI	ATCGAT	1 cut(s)	1640
CsiI	ACCWGGT	1 cut(s)	305
Csp6I	GTAC	7 cut(s)	341, 534, 815, 965, 1022, 1431, 1539
CviAII	CATG	9 cut(s)	68, 239, 251, 699, 1055, 1370, 1375, 1457, 1558
CviQI	GTAC	7 cut(s)	341, 534, 815, 965, 1022, 1431, 1539
DdeI	CTNAG	9 cut(s)	344, 560, 684, 959, 1089, 1166, 1535, 1575, 1623
DinI	GGCGCC	1 cut(s)	909
DpnI	GATC	5 cut(s)	576, 682, 932, 1251, 1639
DpnII	GATC	5 cut(s)	574, 680, 930, 1249, 1637
EaeI	YGGCCR	1 cut(s)	499
Eam1104I	CTCTTC	3 cut(s)	12, 205, 1104
EarI	CTCTTC	3 cut(s)	12, 205, 1104
Eco130I	CCWWGG	2 cut(s)	1456, 1557
Eco24I	GRGCYC	1 cut(s)	410
Eco47I	GGWCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
Eco57I	CTGAAG	1 cut(s)	32
Eco91I	GGTNACC	1 cut(s)	512
EcoO109I	RGGNCCY	1 cut(s)	309
EcoO65I	GGTNACC	1 cut(s)	512
EcoRI	GAATTC	1 cut(s)	1569
EcoRII	CCWGG	7 cut(s)	289, 305, 748, 826, 831, 967, 1629
EcoT14I	CCWWGG	2 cut(s)	1456, 1557
EcoT22I	ATGCAT	1 cut(s)	175
EcoT38I	GRGCYC	1 cut(s)	410
EgeI	GGCGCC	1 cut(s)	909
EheI	GGCGCC	1 cut(s)	909
ErhI	CCWWGG	2 cut(s)	1456, 1557
FaeI	CATG	9 cut(s)	71, 242, 254, 702, 1058, 1373, 1378, 1460, 1561
FalI	AAGNNNNNCTT	4 cut(s)	645, 677, 851, 883
FatI	CATG	9 cut(s)	67, 238, 250, 698, 1054, 1369, 1374, 1456, 1557
FauNDI	CATATG	1 cut(s)	898
FblI	GTMKAC	1 cut(s)	1596
Fnu4HI	GCNGC	8 cut(s)	123, 126, 195, 270, 708, 729, 947, 998
FokI	GGATG	5 cut(s)	259, 788, 1126, 1307, 1349
FriOI	GRGCYC	1 cut(s)	410
Fsp4HI	GCNGC	8 cut(s)	123, 126, 195, 270, 708, 729, 947, 998
FspBI	CTAG	6 cut(s)	363, 419, 1029, 1064, 1542, 1665
FspI	TGCGCA	1 cut(s)	809
GlaI	GCGC	4 cut(s)	809, 909, 1171, 1508
GluI	GCNGC	8 cut(s)	123, 126, 195, 270, 708, 729, 947, 998
GsuI	CTGGAG	3 cut(s)	771, 990, 1266
HaeII	RGCGCY	2 cut(s)	911, 1173
HaeIII	GGCC	2 cut(s)	501, 831
HapII	CCGG	3 cut(s)	509, 937, 1590
HhaI	GCGC	4 cut(s)	810, 910, 1172, 1509
Hin1I	GRCGYC	1 cut(s)	908
Hin1II	CATG	9 cut(s)	71, 242, 254, 702, 1058, 1373, 1378, 1460, 1561
Hin6I	GCGC	4 cut(s)	808, 908, 1170, 1507
HinP1I	GCGC	4 cut(s)	808, 908, 1170, 1507
HincII	GTYRAC	1 cut(s)	603
HindII	GTYRAC	1 cut(s)	603
HindIII	AAGCTT	2 cut(s)	1193, 1270
HinfI	GANTC	5 cut(s)	4, 82, 853, 1450, 1642
HpaII	CCGG	3 cut(s)	509, 937, 1590
HphI	GGTGA	4 cut(s)	239, 410, 560, 893
Hpy166II	GTNNAC	5 cut(s)	341, 603, 965, 1238, 1597
Hpy188III	TCNNGA	8 cut(s)	239, 796, 937, 1099, 1181, 1189, 1370, 1521
Hpy8I	GTNNAC	5 cut(s)	341, 603, 965, 1238, 1597
HpyAV	CCTTC	5 cut(s)	604, 664, 1399, 1421, 1536
HpyCH4III	ACNGT	4 cut(s)	395, 600, 1021, 1384
HpyF10VI	GCNNNNNNNGC	1 cut(s)	868
HpyF3I	CTNAG	9 cut(s)	344, 560, 684, 959, 1089, 1166, 1535, 1575, 1623
Hsp92I	GRCGYC	1 cut(s)	908
Hsp92II	CATG	9 cut(s)	71, 242, 254, 702, 1058, 1373, 1378, 1460, 1561
HspAI	GCGC	4 cut(s)	808, 908, 1170, 1507
KasI	GGCGCC	1 cut(s)	907
Kpn2I	TCCGGA	1 cut(s)	936
Kzo9I	GATC	5 cut(s)	574, 680, 930, 1249, 1637
LmnI	GCTCC	4 cut(s)	262, 422, 752, 1005
Lsp1109I	GCAGC	7 cut(s)	109, 112, 181, 256, 694, 715, 958
LweI	GCATC	7 cut(s)	181, 281, 599, 997, 1148, 1238, 1285
MabI	ACCWGGT	1 cut(s)	305
MaeI	CTAG	6 cut(s)	363, 419, 1029, 1064, 1542, 1665
MaeIII	GTNAC	5 cut(s)	512, 655, 1214, 1378, 1384
MalI	GATC	5 cut(s)	576, 682, 932, 1251, 1639
MboI	GATC	5 cut(s)	574, 680, 930, 1249, 1637
MboII	GAAGA	5 cut(s)	152, 222, 493, 925, 1121
MfeI	CAATTG	1 cut(s)	52
MflI	RGATCY	4 cut(s)	574, 680, 930, 1249
MhlI	GDGCHC	1 cut(s)	410
MluCI	AATT	8 cut(s)	52, 99, 524, 800, 840, 924, 974, 1569
MluI	ACGCGT	1 cut(s)	536
Mly113I	GGCGCC	1 cut(s)	908
MlyI	GAGTC	2 cut(s)	91, 862
MmeI	TCCRAC	2 cut(s)	65, 1615
Mph1103I	ATGCAT	1 cut(s)	175
MroI	TCCGGA	1 cut(s)	936
MseI	TTAA	3 cut(s)	98, 1359, 1512
MslI	CAYNNNNRTG	1 cut(s)	1614
MspI	CCGG	3 cut(s)	509, 937, 1590
MspR9I	CCNGG	9 cut(s)	291, 307, 510, 750, 828, 833, 969, 1590, 1631
MunI	CAATTG	1 cut(s)	52
MvaI	CCWGG	7 cut(s)	291, 307, 750, 828, 833, 969, 1631
MvnI	CGCG	2 cut(s)	208, 538
MwoI	GCNNNNNNNGC	1 cut(s)	868
NarI	GGCGCC	1 cut(s)	908
NciI	CCSGG	2 cut(s)	510, 1590
NcoI	CCATGG	2 cut(s)	1456, 1557
NdeI	CATATG	1 cut(s)	898
NdeII	GATC	5 cut(s)	574, 680, 930, 1249, 1637
NheI	GCTAGC	1 cut(s)	1063
NlaIII	CATG	9 cut(s)	71, 242, 254, 702, 1058, 1373, 1378, 1460, 1561
NlaIV	GGNNCC	5 cut(s)	36, 909, 1001, 1476, 1618
NmeAIII	GCCGAG	1 cut(s)	527
NsbI	TGCGCA	1 cut(s)	809
NsiI	ATGCAT	1 cut(s)	175
NspI	RCATGY	1 cut(s)	702
PagI	TCATGA	2 cut(s)	238, 1369
PciI	ACATGT	1 cut(s)	698
PfeI	GAWTC	2 cut(s)	1450, 1642
PflMI	CCANNNNNTGG	1 cut(s)	1563
PkrI	GCNGC	8 cut(s)	124, 127, 196, 271, 709, 730, 948, 999
PleI	GAGTC	2 cut(s)	90, 861
PluTI	GGCGCC	1 cut(s)	911
PpsI	GAGTC	2 cut(s)	90, 861
PpuMI	RGGWCCY	1 cut(s)	309
PscI	ACATGT	1 cut(s)	698
PshBI	ATTAAT	1 cut(s)	98
Psp5II	RGGWCCY	1 cut(s)	309
Psp6I	CCWGG	7 cut(s)	289, 305, 748, 826, 831, 967, 1629
PspEI	GGTNACC	1 cut(s)	512
PspGI	CCWGG	7 cut(s)	289, 305, 748, 826, 831, 967, 1629
PspN4I	GGNNCC	5 cut(s)	36, 909, 1001, 1476, 1618
PspPI	GGNCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
PspPPI	RGGWCCY	1 cut(s)	309
PstNI	CAGNNNCTG	2 cut(s)	440, 471
PsuI	RGATCY	4 cut(s)	574, 680, 930, 1249
RsaI	GTAC	7 cut(s)	342, 535, 816, 966, 1023, 1432, 1540
RsaNI	GTAC	7 cut(s)	341, 534, 815, 965, 1022, 1431, 1539
RseI	CAYNNNNRTG	1 cut(s)	1614
SaqAI	TTAA	3 cut(s)	98, 1359, 1512
SatI	GCNGC	8 cut(s)	123, 126, 195, 270, 708, 729, 947, 998
Sau3AI	GATC	5 cut(s)	574, 680, 930, 1249, 1637
Sau96I	GGNCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
ScaI	AGTACT	1 cut(s)	1540
SchI	GAGTC	2 cut(s)	91, 862
ScrFI	CCNGG	9 cut(s)	291, 307, 510, 750, 828, 833, 969, 1590, 1631
SduI	GDGCHC	1 cut(s)	410
SexAI	ACCWGGT	1 cut(s)	305
SfaNI	GCATC	7 cut(s)	181, 281, 599, 997, 1148, 1238, 1285
SfcI	CTRYAG	1 cut(s)	1200
SfoI	GGCGCC	1 cut(s)	909
SinI	GGWCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
SmiMI	CAYNNNNRTG	1 cut(s)	1614
SmlI	CTYRAG	1 cut(s)	313
SmoI	CTYRAG	1 cut(s)	313
SpeI	ACTAGT	1 cut(s)	1028
Sse9I	AATT	8 cut(s)	52, 99, 524, 800, 840, 924, 974, 1569
SsiI	CCGC	4 cut(s)	206, 569, 997, 1581
SspDI	GGCGCC	1 cut(s)	907
SspI	AATATT	1 cut(s)	1051
SspMI	CTAG	6 cut(s)	363, 419, 1029, 1064, 1542, 1665
StyD4I	CCNGG	9 cut(s)	289, 305, 508, 748, 826, 831, 967, 1588, 1629
StyI	CCWWGG	2 cut(s)	1456, 1557
TaaI	ACNGT	4 cut(s)	395, 600, 1021, 1384
TaqI	TCGA	3 cut(s)	21, 636, 1640
TasI	AATT	8 cut(s)	52, 99, 524, 800, 840, 924, 974, 1569
TatI	WGTACW	3 cut(s)	340, 814, 1538
TauI	GCSGC	1 cut(s)	1000
TfiI	GAWTC	2 cut(s)	1450, 1642
Tru1I	TTAA	3 cut(s)	98, 1359, 1512
Tru9I	TTAA	3 cut(s)	98, 1359, 1512
TscAI	CASTG	2 cut(s)	45, 967
TseI	GCWGC	7 cut(s)	122, 125, 194, 269, 707, 728, 946
TspDTI	ATGAA	4 cut(s)	537, 902, 1043, 1650
TspGWI	ACGGA	3 cut(s)	759, 992, 1439
TspRI	CASTG	2 cut(s)	45, 967
Van91I	CCANNNNNTGG	1 cut(s)	1563
VpaK11BI	GGWCC	6 cut(s)	35, 309, 506, 746, 1154, 1235
VspI	ATTAAT	1 cut(s)	98
XapI	RAATTY	3 cut(s)	524, 974, 1569
XceI	RCATGY	1 cut(s)	702
XmiI	GTMKAC	1 cut(s)	1596
XspI	CTAG	6 cut(s)	363, 419, 1029, 1064, 1542, 1665
ZrmI	AGTACT	1 cut(s)	1540
Zsp2I	ATGCAT	1 cut(s)	175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.