Rw0G015370

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00696
OG0010845
Physical Location & Seq
Reverse (-)
5820 .. 10394
4575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015370.1

Sequence Viewer

Length: 2076 bp
ATGATGGGGTATGCTAACACGGAGCAGATCGCTTCCGGAGTTCACTTCAGGCTGCGAGCTCGCTCGTTCATTGTGGCGGAGCCGAAGGGCAACCGAGTGGTGTTTGTGAATCTTGATGCTTGCATGGCCTCGCAGCTTGTGAAATTGAAAGTGATTGAGAGGTTGAAGGCAAGGTATGGGGATCTATACACTGAGAAGAATGTAGCTATTAGTGGAATTCACACCCATGCTGGCCCTGGGGGTTATCTACAGTATATTGTGTATATTGTAACATCTCTCGGGTTCGTGCGTCAGTCATTTGATGCCCTTGTTGATGGCATTGAGCAAAGCGTTATTCAAGCCCATGAAAATCTTGCGCCAGGATCAGTTTTTATTAGTAAGGGAGAAATCTTAGATGCTGGAGTAAACCGTAGTCCTAGTGCGTATCTCAATAATCCTGCAGCAGAGCGCAGTAAATACAAATATGATGTCGATAAAGAAATGACTCTTCTGAAGTTTGTCGATAATCAATGGGGTCCAGTGGGTAGCTTCAATTGGTTTGCAACTCATGGAACTTCTATGAGTCGGACAAACTCATTAATTAGTGGGGATAACAAGGGTGCTGCTGCACGATTTATGGAAGACTGGTTTGAGGAGAATGGTGGTAGAAGTGCATATTCTGATGATATTGTGCATATTCTGATGATATTGATGCTGCCGATATACCGCGAAGAGTACCATGAGTTACTGGAGCTTGCTGCATCCTTTCAGTCTCCTCCTGGTAAGCCAGCAACCAGGTCCTTGAGTGTTGCAAGACGTGTCAGGGGTGTACTAAGGCAGGCTAACAAGCCTAGATTTGTTTCTGCCTTTTGTCAATCAAACTGTGGTGATGTGAGCCCAAATGTTCTAGGAGCTTTCTGCACAGATACTGGGCTACCTTGTGATTTCAATCACAGCACCTGTGGTGGGAAGAATGAGTTGTGCTATGGCCGAGGACCGGGGCAACTGAGAAAAGCTGTGGATCTTTTCAACAAAGCATTCAACAAAGCATCTGAACAGTTGACCGGGAAGATTGAATATCGCCACACTTACCTCAACTTCTCCCAGCTTGAAGTAACCCTTCCCAAGAAGGGGGGAGGTTCTGAGGTGGTAAAGACATGTCCTGCTGCAATGGGATTTGGATTTGCTGCTGGAACCACTGATGGACCTGGAGCTTTTGATTTCAAGCAAGGGGATGATAAGGGAAATCCTTTCTGGAGATTAGTGCGCAATGTACTCAAAACACCAGGCCAGGAACAAATTGATTGTCAGAGTCCAAAGCCAATCTTGCTTGATACTGGTGAAATGAAGCAACCATATGACTGGGCGCCTTCAATACTTCCAATTCAGATCTTCCGGATAGGGCAGCTTGTCATTCTCAGTGTACCTGGAGAATTTACAACAATGGCTGGTAGGCGGCTCCGTGATGCTGTGAAAACCGTACTGACTAGTGCTGGTCATGGTGGAAATATTCACGTTGTGCTAGCAGGATTGACTAATACTTATTCTCAGTATATTACGACCTTTGAAGAGTACGAAGTGCAGAGATATGAGGGTGCATCCACTCTCTATGGACCACACACGCTAAGTGCCTACATTCAGGAGTTCAAGAAGCTTGCTACAGCTCTCATCAGTAACCAACCTGTCGCATCAGGTCCACAACCCCCAGATCTCCTTGATAAGCAGATAAGCTTGCTTACTCCAGTTTTTTGGCCTCAAAACTCCACCTTTAAGAGAGGTCATGACATGGTAACAGTTACCTTCTGGTCGGCTTGTCCTCGGAATGACCTATTGACGGAAGGTACATTTGCCCTTGTGGAGATTCTCCATGGAAAGGATACTTGGGTTCCAGCTTACGATGACGATGATTTCTGTCTGCGCTTTAAGTGGTCAAGACCTTCCAAACTCAGTACTAGAAGTCAGGCCACCATGGAATGGAGAATTCCTCAGTCCGCAACTCCCGGCGTATACAGAATTAGGCATTTTGGTGCCTCCAAGAGCCTGGTTGGATCGATTCGCCACTTTACGGGTTCATCTAGTGCTTTTGTGGTAGCATAA

Protein Analysis

691

Amino Acids

76.31

Weight (kDa)

8.74

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 1 - 540 3.6e-207 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 542 - 690 2.2e-40 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1247
AccB1I GGYRCC 2 cut(s) 1345, 2006
AccB7I CCANNNNNTGG 1 cut(s) 1953
AccI GTMKAC 1 cut(s) 1986
AccII CGCG 1 cut(s) 708
AccIII TCCGGA 2 cut(s) 35, 1374
AciI CCGC 4 cut(s) 77, 706, 1435, 1971
AclWI GGATC 4 cut(s) 189, 370, 1008, 2035
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 3 cut(s) 216, 1412, 1959
AcuI CTGAAG 2 cut(s) 31, 512
AcyI GRCGYC 1 cut(s) 1346
AdeI CACNNNGTG 1 cut(s) 1498
AfaI GTAC 8 cut(s) 716, 810, 1254, 1404, 1461, 1553, 1822, 1930
AfiI CCNNNNNNNGG 8 cut(s) 945, 976, 1109, 1110, 1813, 1852, 1953, 2044
AflIII ACRYGT 2 cut(s) 796, 1136
AhlI ACTAGT 1 cut(s) 1466
AjiI CACGTC 1 cut(s) 797
AjnI CCWGG 9 cut(s) 235, 358, 757, 773, 1186, 1264, 1269, 1405, 2019
AjuI GAANNNNNNNTTGG 2 cut(s) 1843, 1875
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 1 cut(s) 756
AlwI GGATC 4 cut(s) 189, 370, 1008, 2035
AlwNI CAGNNNCTG 2 cut(s) 908, 939
Ama87I CYCGRG 1 cut(s) 278
Aor13HI TCCGGA 2 cut(s) 35, 1374
AoxI GGCC 6 cut(s) 126, 232, 967, 1267, 1730, 1941
ApoI RAATTY 3 cut(s) 216, 1412, 1959
ArsI GACNNNNNNTTYG 4 cut(s) 453, 485, 1270, 1302
AseI ATTAAT 1 cut(s) 578
AspLEI GCGC 5 cut(s) 358, 450, 1248, 1348, 1899
AspS9I GGNCC 7 cut(s) 233, 515, 777, 974, 1184, 1592, 1673
AsuC2I CCSGG 3 cut(s) 978, 1045, 1980
AsuHPI GGTGA 2 cut(s) 878, 1331
AsuNHI GCTAGC 1 cut(s) 1501
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 6 cut(s) 515, 777, 974, 1184, 1592, 1673
BaeI ACNNNNGTAYC 2 cut(s) 1848, 1881
BanI GGYRCC 2 cut(s) 1345, 2006
BanII GRGCYC 2 cut(s) 61, 878
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 1 cut(s) 61
BccI CCATC 2 cut(s) 308, 1175
BciT130I CCWGG 9 cut(s) 237, 360, 759, 775, 1188, 1266, 1271, 1407, 2021
BciVI GTATCC 1 cut(s) 1849
BcnI CCSGG 3 cut(s) 978, 1045, 1980
BcoDI GTCTC 1 cut(s) 756
BcuI ACTAGT 1 cut(s) 1466
BfaI CTAG 7 cut(s) 417, 831, 887, 1467, 1502, 1932, 2055
BfmI CTRYAG 3 cut(s) 248, 438, 1638
BfoI RGCGCY 1 cut(s) 1349
BfuI GTATCC 1 cut(s) 1849
BglII AGATCT 2 cut(s) 1368, 1687
BmcAI AGTACT 1 cut(s) 1930
Bme18I GGWCC 6 cut(s) 515, 777, 974, 1184, 1592, 1673
BmeT110I CYCGRG 1 cut(s) 278
BmgBI CACGTC 1 cut(s) 797
BmgT120I GGNCC 7 cut(s) 233, 515, 777, 974, 1184, 1592, 1673
BmiI GGNNCC 7 cut(s) 81, 516, 1174, 1347, 1439, 1866, 2008
BmrI ACTGGG 2 cut(s) 918, 1351
BmsI GCATC 9 cut(s) 106, 292, 385, 681, 749, 1037, 1435, 1586, 1676
BmtI GCTAGC 1 cut(s) 1505
BmuI ACTGGG 2 cut(s) 918, 1351
BpiI GAAGAC 1 cut(s) 627
BplI GAGNNNNNCTC 2 cut(s) 1948, 1980
BpmI CTGGAG 6 cut(s) 420, 749, 1209, 1255, 1428, 1704
BpuEI CTTGAG 1 cut(s) 802
BpuMI CCSGG 3 cut(s) 978, 1045, 1980
Bsa29I ATCGAT 1 cut(s) 2030
BsaBI GATNNNNATC 1 cut(s) 927
BsaHI GRCGYC 1 cut(s) 1346
BsaJI CCNNGG 7 cut(s) 235, 236, 970, 977, 1796, 1846, 1947
BsaWI WCCGGW 2 cut(s) 35, 1374
Bsc4I CCNNNNNNNGG 8 cut(s) 945, 976, 1109, 1110, 1813, 1852, 1953, 2044
Bse1I ACTGG 7 cut(s) 518, 629, 732, 913, 1321, 1346, 1721
Bse3DI GCAATG 2 cut(s) 1155, 1255
Bse8I GATNNNNATC 1 cut(s) 927
BseAI TCCGGA 2 cut(s) 35, 1374
BseBI CCWGG 9 cut(s) 237, 360, 759, 775, 1188, 1266, 1271, 1407, 2021
BseCI ATCGAT 1 cut(s) 2030
BseDI CCNNGG 7 cut(s) 235, 236, 970, 977, 1796, 1846, 1947
BseGI GGATG 3 cut(s) 740, 1219, 1577
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 8 cut(s) 945, 976, 1109, 1110, 1813, 1852, 1953, 2044
BseMI GCAATG 2 cut(s) 1155, 1255
BseMII CTCAG 7 cut(s) 183, 977, 1113, 1411, 1541, 1939, 1979
BseNI ACTGG 7 cut(s) 518, 629, 732, 913, 1321, 1346, 1721
BseRI GAGGAG 2 cut(s) 647, 744
BseYI CCCAGC 1 cut(s) 1083
BsgI GTGCAG 3 cut(s) 591, 883, 1580
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 6 cut(s) 128, 234, 969, 1269, 1732, 1943
BshNI GGYRCC 2 cut(s) 1345, 2006
BshVI ATCGAT 1 cut(s) 2030
BsiHKAI GWGCWC 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 5 cut(s) 36, 977, 1044, 1375, 1980
BslI CCNNNNNNNGG 8 cut(s) 945, 976, 1109, 1110, 1813, 1852, 1953, 2044
BsmAI GTCTC 1 cut(s) 756
BsmI GAATGC 1 cut(s) 1016
BsnI GGCC 6 cut(s) 128, 234, 969, 1269, 1732, 1943
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 61, 878
Bsp13I TCCGGA 2 cut(s) 35, 1374
Bsp143I GATC 7 cut(s) 27, 181, 362, 1000, 1368, 1687, 2027
Bsp19I CCATGG 2 cut(s) 1846, 1947
BspACI CCGC 4 cut(s) 77, 706, 1435, 1971
BspANI GGCC 6 cut(s) 128, 234, 969, 1269, 1732, 1943
BspCNI CTCAG 7 cut(s) 184, 978, 1114, 1410, 1540, 1938, 1978
BspDI ATCGAT 1 cut(s) 2030
BspEI TCCGGA 2 cut(s) 35, 1374
BspFNI CGCG 1 cut(s) 708
BspHI TCATGA 1 cut(s) 1759
BspLI GGNNCC 7 cut(s) 81, 516, 1174, 1347, 1439, 1866, 2008
BspMAI CTGCAG 1 cut(s) 442
BspOI GCTAGC 1 cut(s) 1505
BspPI GGATC 4 cut(s) 189, 370, 1008, 2035
BspT107I GGYRCC 2 cut(s) 1345, 2006
BsrDI GCAATG 2 cut(s) 1155, 1255
BsrI ACTGG 7 cut(s) 518, 629, 732, 913, 1321, 1346, 1721
BssECI CCNNGG 7 cut(s) 235, 236, 970, 977, 1796, 1846, 1947
BssMI GATC 7 cut(s) 27, 181, 362, 1000, 1368, 1687, 2027
BssNAI GTATAC 1 cut(s) 1987
BssNI GRCGYC 1 cut(s) 1346
BssT1I CCWWGG 2 cut(s) 1846, 1947
Bst1107I GTATAC 1 cut(s) 1987
Bst2UI CCWGG 9 cut(s) 237, 360, 759, 775, 1188, 1266, 1271, 1407, 2021
Bst4CI ACNGT 6 cut(s) 252, 410, 863, 1038, 1459, 1774
Bst6I CTCTTC 3 cut(s) 492, 705, 1542
BstACI GRCGYC 1 cut(s) 1346
BstDSI CCRYGG 2 cut(s) 1846, 1947
BstF5I GGATG 3 cut(s) 740, 1219, 1577
BstFNI CGCG 1 cut(s) 708
BstH2I RGCGCY 1 cut(s) 1349
BstHHI GCGC 5 cut(s) 358, 450, 1248, 1348, 1899
BstKTI GATC 7 cut(s) 30, 184, 365, 1003, 1371, 1690, 2030
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 7 cut(s) 27, 181, 362, 1000, 1368, 1687, 2027
BstMWI GCNNNNNNNGC 2 cut(s) 125, 1306
BstNI CCWGG 9 cut(s) 237, 360, 759, 775, 1188, 1266, 1271, 1407, 2021
BstNSI RCATGY 1 cut(s) 1140
BstSFI CTRYAG 3 cut(s) 248, 438, 1638
BstUI CGCG 1 cut(s) 708
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 4 cut(s) 181, 1000, 1368, 1687
BstXI CCANNNNNNTGG 3 cut(s) 1341, 1728, 2020
BstYI RGATCY 4 cut(s) 181, 1000, 1368, 1687
BstZ17I GTATAC 1 cut(s) 1987
Bsu15I ATCGAT 1 cut(s) 2030
BsuI GTATCC 1 cut(s) 1849
BsuRI GGCC 6 cut(s) 128, 234, 969, 1269, 1732, 1943
BsuTUI ATCGAT 1 cut(s) 2030
BtgI CCRYGG 2 cut(s) 1846, 1947
BtrI CACGTC 1 cut(s) 797
BtsCI GGATG 3 cut(s) 740, 1219, 1577
BtsIMutI CAGTG 4 cut(s) 189, 525, 1176, 1405
CaiI CAGNNNCTG 2 cut(s) 908, 939
CciI TCATGA 1 cut(s) 1759
CfoI GCGC 5 cut(s) 358, 450, 1248, 1348, 1899
Cfr13I GGNCC 7 cut(s) 233, 515, 777, 974, 1184, 1592, 1673
ClaI ATCGAT 1 cut(s) 2030
CseI GACGC 1 cut(s) 278
CsiI ACCWGGT 1 cut(s) 773
Csp6I GTAC 8 cut(s) 715, 809, 1253, 1403, 1460, 1552, 1821, 1929
CviQI GTAC 8 cut(s) 715, 809, 1253, 1403, 1460, 1552, 1821, 1929
DinI GGCGCC 1 cut(s) 1347
DpnI GATC 7 cut(s) 29, 183, 364, 1002, 1370, 1689, 2029
DpnII GATC 7 cut(s) 27, 181, 362, 1000, 1368, 1687, 2027
DraIII CACNNNGTG 1 cut(s) 1498
EaeI YGGCCR 1 cut(s) 967
Eam1104I CTCTTC 3 cut(s) 492, 705, 1542
EarI CTCTTC 3 cut(s) 492, 705, 1542
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 1846, 1947
Eco24I GRGCYC 2 cut(s) 61, 878
Eco47I GGWCC 6 cut(s) 515, 777, 974, 1184, 1592, 1673
Eco53kI GAGCTC 1 cut(s) 59
Eco57I CTGAAG 2 cut(s) 31, 512
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 777
EcoRI GAATTC 2 cut(s) 216, 1959
EcoRII CCWGG 9 cut(s) 235, 358, 757, 773, 1186, 1264, 1269, 1405, 2019
EcoT14I CCWWGG 2 cut(s) 1846, 1947
EcoT38I GRGCYC 2 cut(s) 61, 878
EgeI GGCGCC 1 cut(s) 1347
EheI GGCGCC 1 cut(s) 1347
ErhI CCWWGG 2 cut(s) 1846, 1947
FalI AAGNNNNNCTT 4 cut(s) 1083, 1115, 1289, 1321
FauNDI CATATG 1 cut(s) 1336
FblI GTMKAC 1 cut(s) 1986
FokI GGATG 3 cut(s) 727, 1226, 1564
FriOI GRGCYC 2 cut(s) 61, 878
FspBI CTAG 7 cut(s) 417, 831, 887, 1467, 1502, 1932, 2055
FspI TGCGCA 1 cut(s) 1247
GlaI GCGC 5 cut(s) 357, 449, 1247, 1347, 1898
GsaI CCCAGC 1 cut(s) 1087
GsuI CTGGAG 6 cut(s) 420, 749, 1209, 1255, 1428, 1704
HaeII RGCGCY 1 cut(s) 1349
HaeIII GGCC 6 cut(s) 128, 234, 969, 1269, 1732, 1943
HapII CCGG 5 cut(s) 36, 977, 1044, 1375, 1980
HgaI GACGC 1 cut(s) 278
HhaI GCGC 5 cut(s) 358, 450, 1248, 1348, 1899
Hin1I GRCGYC 1 cut(s) 1346
Hin6I GCGC 5 cut(s) 356, 448, 1246, 1346, 1897
HinP1I GCGC 5 cut(s) 356, 448, 1246, 1346, 1897
HincII GTYRAC 1 cut(s) 1041
HindII GTYRAC 1 cut(s) 1041
HindIII AAGCTT 2 cut(s) 1631, 1708
HinfI GANTC 6 cut(s) 109, 484, 562, 1291, 1840, 2032
HpaII CCGG 5 cut(s) 36, 977, 1044, 1375, 1980
HphI GGTGA 2 cut(s) 878, 1331
Hpy166II GTNNAC 7 cut(s) 43, 406, 809, 1041, 1403, 1676, 1987
Hpy188I TCNGA 9 cut(s) 492, 567, 661, 681, 1033, 1123, 1290, 1368, 1800
Hpy188III TCNNGA 8 cut(s) 36, 113, 1234, 1375, 1619, 1627, 1760, 1911
Hpy8I GTNNAC 7 cut(s) 43, 406, 809, 1041, 1403, 1676, 1987
HpyAV CCTTC 8 cut(s) 79, 160, 1102, 1109, 1359, 1789, 1811, 1926
HpyCH4III ACNGT 6 cut(s) 252, 410, 863, 1038, 1459, 1774
HpyCH4IV ACGT 2 cut(s) 796, 1494
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 1306
HpySE526I ACGT 2 cut(s) 796, 1494
Hsp92I GRCGYC 1 cut(s) 1346
HspAI GCGC 5 cut(s) 356, 448, 1246, 1346, 1897
KasI GGCGCC 1 cut(s) 1345
Kpn2I TCCGGA 2 cut(s) 35, 1374
Kzo9I GATC 7 cut(s) 27, 181, 362, 1000, 1368, 1687, 2027
LmnI GCTCC 6 cut(s) 22, 79, 730, 890, 1190, 1443
LweI GCATC 9 cut(s) 106, 292, 385, 681, 749, 1037, 1435, 1586, 1676
MabI ACCWGGT 1 cut(s) 773
MaeI CTAG 7 cut(s) 417, 831, 887, 1467, 1502, 1932, 2055
MaeII ACGT 2 cut(s) 796, 1494
MaeIII GTNAC 6 cut(s) 268, 723, 1093, 1652, 1768, 1774
MalI GATC 7 cut(s) 29, 183, 364, 1002, 1370, 1689, 2029
MboI GATC 7 cut(s) 27, 181, 362, 1000, 1368, 1687, 2027
MboII GAAGA 8 cut(s) 208, 479, 632, 722, 961, 1060, 1363, 1559
MfeI CAATTG 1 cut(s) 532
MflI RGATCY 4 cut(s) 181, 1000, 1368, 1687
MhlI GDGCHC 2 cut(s) 61, 878
MluCI AATT 9 cut(s) 143, 216, 532, 579, 1278, 1362, 1412, 1959, 1992
Mly113I GGCGCC 1 cut(s) 1346
MlyI GAGTC 3 cut(s) 478, 571, 1300
MmeI TCCRAC 2 cut(s) 545, 2005
MroI TCCGGA 2 cut(s) 35, 1374
MseI TTAA 3 cut(s) 578, 1749, 1902
MslI CAYNNNNRTG 2 cut(s) 225, 2004
MspI CCGG 5 cut(s) 36, 977, 1044, 1375, 1980
MunI CAATTG 1 cut(s) 532
Mva1269I GAATGC 1 cut(s) 1016
MvaI CCWGG 9 cut(s) 237, 360, 759, 775, 1188, 1266, 1271, 1407, 2021
MvnI CGCG 1 cut(s) 708
MwoI GCNNNNNNNGC 2 cut(s) 125, 1306
NarI GGCGCC 1 cut(s) 1346
NciI CCSGG 3 cut(s) 978, 1045, 1980
NcoI CCATGG 2 cut(s) 1846, 1947
NdeI CATATG 1 cut(s) 1336
NdeII GATC 7 cut(s) 27, 181, 362, 1000, 1368, 1687, 2027
NheI GCTAGC 1 cut(s) 1501
NlaIV GGNNCC 7 cut(s) 81, 516, 1174, 1347, 1439, 1866, 2008
NmeAIII GCCGAG 1 cut(s) 995
NsbI TGCGCA 1 cut(s) 1247
NspI RCATGY 1 cut(s) 1140
PagI TCATGA 1 cut(s) 1759
PasI CCCWGGG 1 cut(s) 236
PciI ACATGT 1 cut(s) 1136
PctI GAATGC 1 cut(s) 1016
PfeI GAWTC 3 cut(s) 109, 1840, 2032
PflMI CCANNNNNTGG 1 cut(s) 1953
PleI GAGTC 3 cut(s) 478, 570, 1299
PluTI GGCGCC 1 cut(s) 1349
PpsI GAGTC 3 cut(s) 478, 570, 1299
PpuMI RGGWCCY 1 cut(s) 777
PscI ACATGT 1 cut(s) 1136
PshBI ATTAAT 1 cut(s) 578
Psp124BI GAGCTC 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 777
Psp6I CCWGG 9 cut(s) 235, 358, 757, 773, 1186, 1264, 1269, 1405, 2019
PspFI CCCAGC 1 cut(s) 1083
PspGI CCWGG 9 cut(s) 235, 358, 757, 773, 1186, 1264, 1269, 1405, 2019
PspN4I GGNNCC 7 cut(s) 81, 516, 1174, 1347, 1439, 1866, 2008
PspPI GGNCC 7 cut(s) 233, 515, 777, 974, 1184, 1592, 1673
PspPPI RGGWCCY 1 cut(s) 777
PstI CTGCAG 1 cut(s) 442
PstNI CAGNNNCTG 2 cut(s) 908, 939
PsuI RGATCY 4 cut(s) 181, 1000, 1368, 1687
RsaI GTAC 8 cut(s) 716, 810, 1254, 1404, 1461, 1553, 1822, 1930
RsaNI GTAC 8 cut(s) 715, 809, 1253, 1403, 1460, 1552, 1821, 1929
RseI CAYNNNNRTG 2 cut(s) 225, 2004
SacI GAGCTC 1 cut(s) 61
SaqAI TTAA 3 cut(s) 578, 1749, 1902
Sau3AI GATC 7 cut(s) 27, 181, 362, 1000, 1368, 1687, 2027
Sau96I GGNCC 7 cut(s) 233, 515, 777, 974, 1184, 1592, 1673
ScaI AGTACT 1 cut(s) 1930
SchI GAGTC 3 cut(s) 478, 571, 1300
SduI GDGCHC 2 cut(s) 61, 878
SexAI ACCWGGT 1 cut(s) 773
SfaNI GCATC 9 cut(s) 106, 292, 385, 681, 749, 1037, 1435, 1586, 1676
SfcI CTRYAG 3 cut(s) 248, 438, 1638
SfoI GGCGCC 1 cut(s) 1347
SinI GGWCC 6 cut(s) 515, 777, 974, 1184, 1592, 1673
SmiMI CAYNNNNRTG 2 cut(s) 225, 2004
SmlI CTYRAG 1 cut(s) 781
SmoI CTYRAG 1 cut(s) 781
SpeI ACTAGT 1 cut(s) 1466
Sse9I AATT 9 cut(s) 143, 216, 532, 579, 1278, 1362, 1412, 1959, 1992
SsiI CCGC 4 cut(s) 77, 706, 1435, 1971
SspDI GGCGCC 1 cut(s) 1345
SspI AATATT 1 cut(s) 1489
SspMI CTAG 7 cut(s) 417, 831, 887, 1467, 1502, 1932, 2055
SstI GAGCTC 1 cut(s) 61
StyI CCWWGG 2 cut(s) 1846, 1947
TaaI ACNGT 6 cut(s) 252, 410, 863, 1038, 1459, 1774
TaiI ACGT 2 cut(s) 799, 1497
TaqI TCGA 3 cut(s) 471, 501, 2030
TasI AATT 9 cut(s) 143, 216, 532, 579, 1278, 1362, 1412, 1959, 1992
TatI WGTACW 3 cut(s) 808, 1252, 1928
TauI GCSGC 1 cut(s) 1438
TfiI GAWTC 3 cut(s) 109, 1840, 2032
Tru1I TTAA 3 cut(s) 578, 1749, 1902
Tru9I TTAA 3 cut(s) 578, 1749, 1902
TscAI CASTG 4 cut(s) 196, 525, 1183, 1405
TspDTI ATGAA 4 cut(s) 58, 360, 1340, 2040
TspGWI ACGGA 3 cut(s) 35, 1430, 1829
TspRI CASTG 4 cut(s) 196, 525, 1183, 1405
Van91I CCANNNNNTGG 1 cut(s) 1953
VpaK11BI GGWCC 6 cut(s) 515, 777, 974, 1184, 1592, 1673
VspI ATTAAT 1 cut(s) 578
XapI RAATTY 3 cut(s) 216, 1412, 1959
XceI RCATGY 1 cut(s) 1140
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 1986
XspI CTAG 7 cut(s) 417, 831, 887, 1467, 1502, 1932, 2055
ZrmI AGTACT 1 cut(s) 1930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.