AT5G58980

Neutral/alkaline non-lysosomal ceramidase, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010845
Physical Location & Seq
Reverse (-)
23811449 .. 23814683
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58980.1

Sequence Viewer

Length: 2202 bp
ATGACGAGGTGGTCTATGTCTATGCACTGCACATTGTTCTTGCTATTTCTGTTGAGGCTCACTTGTATATTCTCAGATTCCGATTATTTGATGGGTCTTGGAAGCTATGACATCACAGGACCTGCAGCTGATGTTAACATGATGGGTTATGCTAACATGGAACAAGTAGCATCTGGTGTTCACTTCAGGCTCAGAGCTCGTGCGTTCATCGTGGCTGAGCCATACAAGAAAAGGATTGCTTTTGTTAATCTTGATGCTGGTATGGCCTCACAGCTTGTGACCATCAAAGTGATTGAAAGATTGAAACAGAGGTACGGTGAGCTTTATACGGAGGAAAATGTTGCAATTAGTGGAACTCATACTCATGCTGGTCCAGGGGGTTATCTCCAATACATTTTGTACTTAGTTACATCTCTCGGGTTTGTTCATCAATCTTTCAATGCTCTTGTGGACGGTATAGAGCAAAGTATTATACAAGCTCATGAAAATCTTCGTCCCGGGTCCATTTTAATCAACAAAGGGGAGCTCTTGGATGCTGGTGTGAACCGTAGTCCCAGTGCTTACCTTAACAATCCGGCACATGAACGTAGTAAATATGAATATGATGTAGATAAAGAGATGACACTTGTCAAATTCGTTGATGATCAATGGGGTCCAGTGGCACGTATCATGGAAGACTGGTTTGAACGAGAAAACGGTTGTAGATCAGTTGATGTTGAATCTCCTAGAAGAGTCTCAAGCATAATCAGTGATCCTTATGATCAAGATTTGATGGAAATGGCATCCTCGTTACTGTCTACCGGCGGTAAAACCGTTACAAGGATGTCAAGCGTTGCTAGACGGGTCAGAAGTAGATTTAGACACGCTGACAAACCTAGATTTGTCTCTGCCTTCTGTCAAACTAACTGCGGTGATGTTAGTCCGAATGTGTTGGGAGCCTTCTGTATAGACACAGGCCTTCCTTGTGAATTCAACCAAAGTACTTGTGGTGGAAAGAATGAGCAATGCTACGGCCGTGGACCAGGCTACCCTGATGAATTTGAGAGTACCCGCATTATTGGAGAGAGGCAGTTCAAGAAGGCTGCGGATCTTTTCACTAAAGCCTCTGAGGAGATACAAGGAAAAGTTGACTATCGTCATGCATATGTTGATTTCTCTCAGCTTGAAGTGACGATTAATGGACAAAATGGAGGTTCCGAAGTGGTGAAAACATGTCCAGCTGCAATGGGTTTTGGTTTCGCTGCAGGAACAACTGATGGACCTGGAGCATTTGACTTTAAACAAGGAGATGATCAGGGAAATCCTTTCTGGAGGCTTGTAAGGAACCTTCTCAAGAATCCAACTGAGGAACAAGTACGATGCCAACGACCTAAACCCATATTGCTTGATACCGGTGAAATGAAACAACCATACGACTGGGCGCCGTCGATATTACCAGTTCAGATCCTCCGCATTGGCCAGCTAGTGATTCTCTGCGTCCCCGGAGAATTCACAACAATGGCAGGGAGGCGACTACGTGATGCCGTGAAAACAGTGCTTAAAGAAGGCAGCAATGGAAGAGAATTCAGCGTGGTAATAGCCGGGCTAACCAATTCGTATTCGCAGTATATTGCCACATTTGAGGAATACCAAGTTCAGAGATATGAGGGTGCATCGACTTTGTATGGACCTCACACGCTAAGCGGATACATTCAAGAATTCAAGAAACTAGCAAATGATCTTCTATCTGCTCAAACCACTGACCCGGGTCCCCAACCACCTGACTTACTTCACAAGCAAATAAGCTTACTAACACCTGTTGTTGCGGATATGACACCAATTGGAACTGCATTCGGAGATGTTACATCGGATGTTCCTCGGTTATCCAAATTCAGGAAGGGAGCTGACATAGTGAGGGTTCAATTCCGGTCAGCTAACCCGAGAAACGATCTGATGACGGAAGGAACTTTCGCGCTTGTTGAGAGGTGGCTCGAAGGAAGAGAGACATGGGTACCTGTTTATGATGATGATGACTTTTGCCTCCGGTTTAAGTGGTCAAGACCGTTTAAACTCAGCACGCAGAGCACAGCAACTATCGAATGGAGAATCCCTGAAACCGCATCACCTGGTGTTTATAGAATCACTCACTTTGGTTCAGCTAAAACTCCTATAAGCTCCATTCACCATTTCTCTGGTTCATCTAGTGCCTTTGTTGTATATTGA

Protein Analysis

733

Amino Acids

81.84

Weight (kDa)

6.21

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 29 - 565 3.3e-207 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 567 - 732 8.3e-54 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
Acc36I ACCTGC 1 cut(s) 130
Acc65I GGTACC 1 cut(s) 1990
AccB1I GGYRCC 2 cut(s) 1420, 1990
AccI GTMKAC 1 cut(s) 797
AccII CGCG 1 cut(s) 1952
AciI CCGC 8 cut(s) 804, 909, 1051, 1085, 1450, 1683, 1805, 2097
AclWI GGATC 3 cut(s) 746, 1095, 1438
AcoI YGGCCR 2 cut(s) 1012, 1456
AcsI RAATTY 7 cut(s) 632, 968, 1037, 1487, 1562, 1697, 1868
AcuI CTGAAG 1 cut(s) 169
AcyI GRCGYC 1 cut(s) 1421
AdeI CACNNNGTG 1 cut(s) 2108
AfaI GTAC 6 cut(s) 314, 401, 982, 1048, 1356, 1992
AfiI CCNNNNNNNGG 2 cut(s) 819, 1872
AflIII ACRYGT 1 cut(s) 1211
AgeI ACCGGT 1 cut(s) 1391
AjnI CCWGG 4 cut(s) 373, 1021, 1261, 2104
Alw21I GWGCWC 3 cut(s) 199, 528, 2066
Alw26I GTCTC 3 cut(s) 739, 889, 1976
AlwI GGATC 3 cut(s) 746, 1095, 1438
AlwNI CAGNNNCTG 1 cut(s) 122
Ama87I CYCGRG 4 cut(s) 416, 497, 1744, 1918
AoxI GGCC 4 cut(s) 264, 955, 1012, 1456
ApeKI GCWGC 5 cut(s) 125, 1082, 1220, 1241, 1548
ApoI RAATTY 7 cut(s) 632, 968, 1037, 1487, 1562, 1697, 1868
AseI ATTAAT 1 cut(s) 1176
AsiGI ACCGGT 1 cut(s) 1391
Asp700I GAANNNNTTC 1 cut(s) 489
Asp718I GGTACC 1 cut(s) 1990
AspLEI GCGC 2 cut(s) 1423, 1954
AspS9I GGNCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
AsuC2I CCSGG 6 cut(s) 498, 499, 1482, 1582, 1745, 1746
AsuHPI GGTGA 6 cut(s) 329, 923, 1216, 1406, 2094, 2153
AvaI CYCGRG 4 cut(s) 416, 497, 1744, 1918
AvaII GGWCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
BaeI ACNNNNGTAYC 2 cut(s) 1974, 2007
BalI TGGCCA 1 cut(s) 1458
BanI GGYRCC 2 cut(s) 1420, 1990
BanII GRGCYC 2 cut(s) 199, 528
BarI GAAGNNNNNNTAC 2 cut(s) 1311, 1343
BauI CACGAG 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 681
Bbv12I GWGCWC 3 cut(s) 199, 528, 2066
BbvI GCAGC 5 cut(s) 137, 1069, 1207, 1228, 1560
BccI CCATC 5 cut(s) 85, 136, 290, 766, 1250
BceAI ACGGC 4 cut(s) 999, 1027, 1408, 1508
BciT130I CCWGG 4 cut(s) 375, 1023, 1263, 2106
BciVI GTATCC 1 cut(s) 1679
BclI TGATCA 3 cut(s) 643, 760, 1291
BcnI CCSGG 6 cut(s) 498, 499, 1482, 1582, 1745, 1746
BcoDI GTCTC 3 cut(s) 739, 889, 1976
BfaI CTAG 6 cut(s) 726, 837, 876, 1463, 1709, 2181
BfmI CTRYAG 2 cut(s) 123, 1242
BfoI RGCGCY 1 cut(s) 1424
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 1 cut(s) 1679
BisI GCNGC 5 cut(s) 126, 1083, 1221, 1242, 1549
BlpI GCTNAGC 2 cut(s) 216, 1679
BlsI GCNGC 5 cut(s) 127, 1084, 1222, 1243, 1550
BmcAI AGTACT 1 cut(s) 982
Bme18I GGWCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
BmeT110I CYCGRG 4 cut(s) 416, 497, 1744, 1918
BmgT120I GGNCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
BmiI GGNNCC 9 cut(s) 502, 654, 937, 1195, 1325, 1422, 1749, 1750, 1992
BmrI ACTGGG 2 cut(s) 549, 1426
BmsI GCATC 8 cut(s) 179, 244, 523, 791, 1349, 1510, 1661, 2108
BmuI ACTGGG 2 cut(s) 549, 1426
BoxI GACNNNNGTC 3 cut(s) 626, 1134, 1746
BpiI GAAGAC 1 cut(s) 681
BpmI CTGGAG 2 cut(s) 1284, 1330
Bpu1102I GCTNAGC 2 cut(s) 216, 1679
BpuEI CTTGAG 2 cut(s) 721, 1316
BpuMI CCSGG 6 cut(s) 498, 499, 1482, 1582, 1745, 1746
BsaAI YACGTR 2 cut(s) 665, 1517
BsaHI GRCGYC 1 cut(s) 1421
BsaJI CCNNGG 6 cut(s) 374, 497, 1015, 1480, 1744, 1856
BsaWI WCCGGW 3 cut(s) 1391, 1905, 2022
Bsc4I CCNNNNNNNGG 2 cut(s) 819, 1872
Bse118I RCCGGY 2 cut(s) 800, 1391
Bse1I ACTGG 5 cut(s) 555, 656, 683, 1421, 1436
Bse3DI GCAATG 3 cut(s) 1010, 1230, 1558
BseBI CCWGG 4 cut(s) 375, 1023, 1263, 2106
BseDI CCNNGG 6 cut(s) 374, 497, 1015, 1480, 1744, 1856
BseGI GGATG 4 cut(s) 538, 782, 828, 1855
BseLI CCNNNNNNNGG 2 cut(s) 819, 1872
BseMI GCAATG 3 cut(s) 1010, 1230, 1558
BseMII CTCAG 7 cut(s) 87, 205, 207, 1098, 1172, 1335, 2065
BseNI ACTGG 5 cut(s) 555, 656, 683, 1421, 1436
BseRI GAGGAG 1 cut(s) 1124
BseX3I CGGCCG 1 cut(s) 1012
BseXI GCAGC 5 cut(s) 137, 1069, 1207, 1228, 1560
BsgI GTGCAG 1 cut(s) 13
Bsh1236I CGCG 1 cut(s) 1952
Bsh1285I CGRYCG 1 cut(s) 1015
BshFI GGCC 4 cut(s) 266, 957, 1014, 1458
BshNI GGYRCC 2 cut(s) 1420, 1990
BshTI ACCGGT 1 cut(s) 1391
BsiEI CGRYCG 1 cut(s) 1015
BsiHKAI GWGCWC 3 cut(s) 199, 528, 2066
BsiHKCI CYCGRG 4 cut(s) 416, 497, 1744, 1918
BsiSI CCGG 9 cut(s) 498, 575, 801, 1392, 1482, 1581, 1745, 1906, 2023
BslFI GGGAC 4 cut(s) 480, 537, 1463, 1734
BslI CCNNNNNNNGG 2 cut(s) 819, 1872
BsmAI GTCTC 3 cut(s) 739, 889, 1976
BsmFI GGGAC 4 cut(s) 480, 537, 1463, 1734
BsmI GAATGC 1 cut(s) 1829
BsnI GGCC 4 cut(s) 266, 957, 1014, 1458
BsoBI CYCGRG 4 cut(s) 416, 497, 1744, 1918
Bsp1286I GDGCHC 3 cut(s) 199, 528, 2066
Bsp143I GATC 9 cut(s) 643, 704, 751, 760, 1087, 1291, 1443, 1717, 1927
Bsp1720I GCTNAGC 2 cut(s) 216, 1679
BspACI CCGC 8 cut(s) 804, 909, 1051, 1085, 1450, 1683, 1805, 2097
BspANI GGCC 4 cut(s) 266, 957, 1014, 1458
BspCNI CTCAG 7 cut(s) 86, 204, 208, 1099, 1171, 1336, 2064
BspFNI CGCG 1 cut(s) 1952
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 9 cut(s) 502, 654, 937, 1195, 1325, 1422, 1749, 1750, 1992
BspMAI CTGCAG 2 cut(s) 127, 1246
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 3 cut(s) 746, 1095, 1438
BspT107I GGYRCC 2 cut(s) 1420, 1990
BsrDI GCAATG 3 cut(s) 1010, 1230, 1558
BsrFI RCCGGY 2 cut(s) 800, 1391
BsrI ACTGG 5 cut(s) 555, 656, 683, 1421, 1436
BssAI RCCGGY 2 cut(s) 800, 1391
BssECI CCNNGG 6 cut(s) 374, 497, 1015, 1480, 1744, 1856
BssMI GATC 9 cut(s) 643, 704, 751, 760, 1087, 1291, 1443, 1717, 1927
BssNI GRCGYC 1 cut(s) 1421
BssSI CACGAG 1 cut(s) 198
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 4 cut(s) 375, 1023, 1263, 2106
Bst4CI ACNGT 8 cut(s) 317, 455, 548, 698, 795, 814, 1534, 2043
Bst6I CTCTTC 3 cut(s) 724, 1552, 1972
BstACI GRCGYC 1 cut(s) 1421
BstBAI YACGTR 2 cut(s) 665, 1517
BstC8I GCNNGC 2 cut(s) 1460, 2057
BstDEI CTNAG 9 cut(s) 73, 191, 216, 403, 1107, 1158, 1344, 1679, 2051
BstDSI CCRYGG 1 cut(s) 1015
BstF5I GGATG 4 cut(s) 538, 782, 828, 1855
BstFNI CGCG 1 cut(s) 1952
BstH2I RGCGCY 1 cut(s) 1424
BstHHI GCGC 2 cut(s) 1423, 1954
BstKTI GATC 9 cut(s) 646, 707, 754, 763, 1090, 1294, 1446, 1720, 1930
BstMAI GTCTC 3 cut(s) 739, 889, 1976
BstMBI GATC 9 cut(s) 643, 704, 751, 760, 1087, 1291, 1443, 1717, 1927
BstMCI CGRYCG 1 cut(s) 1015
BstMWI GCNNNNNNNGC 2 cut(s) 263, 2061
BstNI CCWGG 4 cut(s) 375, 1023, 1263, 2106
BstNSI RCATGY 1 cut(s) 1215
BstPAI GACNNNNGTC 3 cut(s) 626, 1134, 1746
BstSFI CTRYAG 2 cut(s) 123, 1242
BstUI CGCG 1 cut(s) 1952
BstV1I GCAGC 5 cut(s) 137, 1069, 1207, 1228, 1560
BstV2I GAAGAC 1 cut(s) 681
BstX2I RGATCY 2 cut(s) 1087, 1443
BstXI CCANNNNNNTGG 2 cut(s) 1416, 2171
BstYI RGATCY 2 cut(s) 1087, 1443
BstZI CGGCCG 1 cut(s) 1012
BsuI GTATCC 1 cut(s) 1679
BsuRI GGCC 4 cut(s) 266, 957, 1014, 1458
BtgI CCRYGG 1 cut(s) 1015
BtsCI GGATG 4 cut(s) 538, 782, 828, 1855
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 6 cut(s) 25, 562, 663, 754, 1539, 1737
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 2 cut(s) 1460, 2057
CaiI CAGNNNCTG 1 cut(s) 122
CciI TCATGA 1 cut(s) 481
CfoI GCGC 2 cut(s) 1423, 1954
Cfr10I RCCGGY 2 cut(s) 800, 1391
Cfr13I GGNCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
Cfr9I CCCGGG 2 cut(s) 497, 1744
CseI GACGC 1 cut(s) 1465
CsiI ACCWGGT 1 cut(s) 2104
Csp6I GTAC 6 cut(s) 313, 400, 981, 1047, 1355, 1991
CspAI ACCGGT 1 cut(s) 1391
CviAII CATG 9 cut(s) 139, 157, 365, 482, 581, 670, 1139, 1212, 1986
CviQI GTAC 6 cut(s) 313, 400, 981, 1047, 1355, 1991
DdeI CTNAG 9 cut(s) 73, 191, 216, 403, 1107, 1158, 1344, 1679, 2051
DinI GGCGCC 1 cut(s) 1422
DpnI GATC 9 cut(s) 645, 706, 753, 762, 1089, 1293, 1445, 1719, 1929
DpnII GATC 9 cut(s) 643, 704, 751, 760, 1087, 1291, 1443, 1717, 1927
DraI TTTAAA 2 cut(s) 1279, 2047
DraIII CACNNNGTG 1 cut(s) 2108
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 2 cut(s) 1012, 1456
EagI CGGCCG 1 cut(s) 1012
Eam1104I CTCTTC 3 cut(s) 724, 1552, 1972
EarI CTCTTC 3 cut(s) 724, 1552, 1972
Ecl136II GAGCTC 2 cut(s) 197, 526
EclXI CGGCCG 1 cut(s) 1012
Eco147I AGGCCT 1 cut(s) 957
Eco24I GRGCYC 2 cut(s) 199, 528
Eco47I GGWCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
Eco52I CGGCCG 1 cut(s) 1012
Eco53kI GAGCTC 2 cut(s) 197, 526
Eco57I CTGAAG 1 cut(s) 169
Eco88I CYCGRG 4 cut(s) 416, 497, 1744, 1918
EcoICRI GAGCTC 2 cut(s) 197, 526
EcoO109I RGGNCCY 2 cut(s) 119, 1748
EcoRI GAATTC 4 cut(s) 968, 1487, 1562, 1697
EcoRII CCWGG 4 cut(s) 373, 1021, 1261, 2104
EcoT22I ATGCAT 1 cut(s) 1144
EcoT38I GRGCYC 2 cut(s) 199, 528
EgeI GGCGCC 1 cut(s) 1422
EheI GGCGCC 1 cut(s) 1422
FaeI CATG 9 cut(s) 142, 160, 368, 485, 584, 673, 1142, 1215, 1989
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FaqI GGGAC 4 cut(s) 480, 537, 1463, 1734
FatI CATG 9 cut(s) 138, 156, 364, 481, 580, 669, 1138, 1211, 1985
FauI CCCGC 1 cut(s) 1058
FauNDI CATATG 1 cut(s) 1144
FbaI TGATCA 3 cut(s) 643, 760, 1291
FblI GTMKAC 1 cut(s) 797
Fnu4HI GCNGC 5 cut(s) 126, 1083, 1221, 1242, 1549
FokI GGATG 4 cut(s) 545, 769, 835, 1862
FriOI GRGCYC 2 cut(s) 199, 528
Fsp4HI GCNGC 5 cut(s) 126, 1083, 1221, 1242, 1549
FspBI CTAG 6 cut(s) 726, 837, 876, 1463, 1709, 2181
GlaI GCGC 2 cut(s) 1422, 1953
GluI GCNGC 5 cut(s) 126, 1083, 1221, 1242, 1549
GsuI CTGGAG 2 cut(s) 1284, 1330
HaeII RGCGCY 1 cut(s) 1424
HaeIII GGCC 4 cut(s) 266, 957, 1014, 1458
HapII CCGG 9 cut(s) 498, 575, 801, 1392, 1482, 1581, 1745, 1906, 2023
HgaI GACGC 1 cut(s) 1465
HhaI GCGC 2 cut(s) 1423, 1954
Hin1I GRCGYC 1 cut(s) 1421
Hin1II CATG 9 cut(s) 142, 160, 368, 485, 584, 673, 1142, 1215, 1989
Hin6I GCGC 2 cut(s) 1421, 1952
HinP1I GCGC 2 cut(s) 1421, 1952
HincII GTYRAC 2 cut(s) 136, 1129
HindII GTYRAC 2 cut(s) 136, 1129
HindIII AAGCTT 1 cut(s) 1783
HinfI GANTC 7 cut(s) 77, 719, 732, 1336, 1468, 2085, 2118
HpaI GTTAAC 1 cut(s) 136
HpaII CCGG 9 cut(s) 498, 575, 801, 1392, 1482, 1581, 1745, 1906, 2023
HphI GGTGA 6 cut(s) 329, 923, 1216, 1406, 2094, 2153
Hpy166II GTNNAC 7 cut(s) 136, 181, 451, 544, 798, 1019, 1129
Hpy8I GTNNAC 7 cut(s) 136, 181, 451, 544, 798, 1019, 1129
Hpy99I CGWCG 1 cut(s) 1429
HpyAV CCTTC 9 cut(s) 901, 949, 968, 1072, 1337, 1538, 1870, 1934, 1967
HpyCH4III ACNGT 8 cut(s) 317, 455, 548, 698, 795, 814, 1534, 2043
HpyCH4IV ACGT 3 cut(s) 586, 664, 1516
HpyCH4V TGCA 9 cut(s) 25, 30, 125, 344, 1142, 1223, 1244, 1652, 1829
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 2061
HpyF3I CTNAG 9 cut(s) 73, 191, 216, 403, 1107, 1158, 1344, 1679, 2051
HpySE526I ACGT 3 cut(s) 586, 664, 1516
Hsp92I GRCGYC 1 cut(s) 1421
Hsp92II CATG 9 cut(s) 142, 160, 368, 485, 584, 673, 1142, 1215, 1989
HspAI GCGC 2 cut(s) 1421, 1952
KasI GGCGCC 1 cut(s) 1420
KflI GGGWCCC 1 cut(s) 1748
KpnI GGTACC 1 cut(s) 1994
Ksp22I TGATCA 3 cut(s) 643, 760, 1291
KspAI GTTAAC 1 cut(s) 136
Kzo9I GATC 9 cut(s) 643, 704, 751, 760, 1087, 1291, 1443, 1717, 1927
LmnI GCTCC 5 cut(s) 523, 935, 1265, 1880, 2159
Lsp1109I GCAGC 5 cut(s) 137, 1069, 1207, 1228, 1560
LweI GCATC 8 cut(s) 179, 244, 523, 791, 1349, 1510, 1661, 2108
MabI ACCWGGT 1 cut(s) 2104
MaeI CTAG 6 cut(s) 726, 837, 876, 1463, 1709, 2181
MaeII ACGT 3 cut(s) 586, 664, 1516
MaeIII GTNAC 6 cut(s) 277, 406, 789, 814, 1168, 1840
MalI GATC 9 cut(s) 645, 706, 753, 762, 1089, 1293, 1445, 1719, 1929
MboI GATC 9 cut(s) 643, 704, 751, 760, 1087, 1291, 1443, 1717, 1927
MboII GAAGA 6 cut(s) 482, 686, 741, 1569, 1712, 1989
MfeI CAATTG 1 cut(s) 1818
MflI RGATCY 2 cut(s) 1087, 1443
MhlI GDGCHC 3 cut(s) 199, 528, 2066
MlsI TGGCCA 1 cut(s) 1458
MluNI TGGCCA 1 cut(s) 1458
Mly113I GGCGCC 1 cut(s) 1421
MlyI GAGTC 1 cut(s) 741
MmeI TCCRAC 1 cut(s) 1364
Mox20I TGGCCA 1 cut(s) 1458
Mph1103I ATGCAT 1 cut(s) 1144
MroXI GAANNNNTTC 1 cut(s) 489
MscI TGGCCA 1 cut(s) 1458
MseI TTAA 9 cut(s) 135, 246, 509, 567, 1176, 1278, 1539, 2028, 2046
MslI CAYNNNNRTG 4 cut(s) 287, 363, 1143, 1496
Msp20I TGGCCA 1 cut(s) 1458
MspA1I CMGCKG 2 cut(s) 128, 1220
MspI CCGG 9 cut(s) 498, 575, 801, 1392, 1482, 1581, 1745, 1906, 2023
MssI GTTTAAAC 1 cut(s) 2047
MunI CAATTG 1 cut(s) 1818
Mva1269I GAATGC 1 cut(s) 1829
MvaI CCWGG 4 cut(s) 375, 1023, 1263, 2106
MvnI CGCG 1 cut(s) 1952
MwoI GCNNNNNNNGC 2 cut(s) 263, 2061
NarI GGCGCC 1 cut(s) 1421
NciI CCSGG 6 cut(s) 498, 499, 1482, 1582, 1745, 1746
NdeI CATATG 1 cut(s) 1144
NdeII GATC 9 cut(s) 643, 704, 751, 760, 1087, 1291, 1443, 1717, 1927
NlaIII CATG 9 cut(s) 142, 160, 368, 485, 584, 673, 1142, 1215, 1989
NlaIV GGNNCC 9 cut(s) 502, 654, 937, 1195, 1325, 1422, 1749, 1750, 1992
NmuCI GTSAC 2 cut(s) 277, 1168
NsiI ATGCAT 1 cut(s) 1144
NspI RCATGY 1 cut(s) 1215
PagI TCATGA 1 cut(s) 481
PceI AGGCCT 1 cut(s) 957
PciI ACATGT 1 cut(s) 1211
PcsI WCGNNNNNNNCGW 1 cut(s) 1363
PctI GAATGC 1 cut(s) 1829
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 6 cut(s) 77, 719, 1336, 1468, 2085, 2118
PinAI ACCGGT 1 cut(s) 1391
PkrI GCNGC 5 cut(s) 127, 1084, 1222, 1243, 1550
PleI GAGTC 1 cut(s) 740
PluTI GGCGCC 1 cut(s) 1424
PmeI GTTTAAAC 1 cut(s) 2047
PpsI GAGTC 1 cut(s) 740
Ppu21I YACGTR 2 cut(s) 665, 1517
PpuMI RGGWCCY 2 cut(s) 119, 1748
PscI ACATGT 1 cut(s) 1211
PshAI GACNNNNGTC 3 cut(s) 626, 1134, 1746
PshBI ATTAAT 1 cut(s) 1176
Psp124BI GAGCTC 2 cut(s) 199, 528
Psp5II RGGWCCY 2 cut(s) 119, 1748
Psp6I CCWGG 4 cut(s) 373, 1021, 1261, 2104
PspGI CCWGG 4 cut(s) 373, 1021, 1261, 2104
PspN4I GGNNCC 9 cut(s) 502, 654, 937, 1195, 1325, 1422, 1749, 1750, 1992
PspPI GGNCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
PspPPI RGGWCCY 2 cut(s) 119, 1748
PstI CTGCAG 2 cut(s) 127, 1246
PstNI CAGNNNCTG 1 cut(s) 122
PsuI RGATCY 2 cut(s) 1087, 1443
PvuII CAGCTG 2 cut(s) 128, 1220
RsaI GTAC 6 cut(s) 314, 401, 982, 1048, 1356, 1992
RsaNI GTAC 6 cut(s) 313, 400, 981, 1047, 1355, 1991
RseI CAYNNNNRTG 4 cut(s) 287, 363, 1143, 1496
SacI GAGCTC 2 cut(s) 199, 528
SaqAI TTAA 9 cut(s) 135, 246, 509, 567, 1176, 1278, 1539, 2028, 2046
SatI GCNGC 5 cut(s) 126, 1083, 1221, 1242, 1549
Sau3AI GATC 9 cut(s) 643, 704, 751, 760, 1087, 1291, 1443, 1717, 1927
Sau96I GGNCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
ScaI AGTACT 1 cut(s) 982
SchI GAGTC 1 cut(s) 741
SduI GDGCHC 3 cut(s) 199, 528, 2066
SexAI ACCWGGT 1 cut(s) 2104
SfaNI GCATC 8 cut(s) 179, 244, 523, 791, 1349, 1510, 1661, 2108
SfcI CTRYAG 2 cut(s) 123, 1242
SfoI GGCGCC 1 cut(s) 1422
SinI GGWCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
SmaI CCCGGG 2 cut(s) 499, 1746
SmiMI CAYNNNNRTG 4 cut(s) 287, 363, 1143, 1496
SmlI CTYRAG 2 cut(s) 736, 1331
SmoI CTYRAG 2 cut(s) 736, 1331
SseBI AGGCCT 1 cut(s) 957
SsiI CCGC 8 cut(s) 804, 909, 1051, 1085, 1450, 1683, 1805, 2097
SspDI GGCGCC 1 cut(s) 1420
SspMI CTAG 6 cut(s) 726, 837, 876, 1463, 1709, 2181
SstI GAGCTC 2 cut(s) 199, 528
StuI AGGCCT 1 cut(s) 957
TaaI ACNGT 8 cut(s) 317, 455, 548, 698, 795, 814, 1534, 2043
TaiI ACGT 3 cut(s) 589, 667, 1519
TaqI TCGA 4 cut(s) 1427, 1655, 1971, 2076
TatI WGTACW 2 cut(s) 399, 980
TfiI GAWTC 6 cut(s) 77, 719, 1336, 1468, 2085, 2118
Tru1I TTAA 9 cut(s) 135, 246, 509, 567, 1176, 1278, 1539, 2028, 2046
Tru9I TTAA 9 cut(s) 135, 246, 509, 567, 1176, 1278, 1539, 2028, 2046
TscAI CASTG 6 cut(s) 32, 562, 663, 754, 1539, 1744
TseFI GTSAC 2 cut(s) 277, 1168
TseI GCWGC 5 cut(s) 125, 1082, 1220, 1241, 1548
Tsp45I GTSAC 2 cut(s) 277, 1168
TspDTI ATGAA 8 cut(s) 196, 416, 498, 597, 612, 1050, 1415, 2166
TspGWI ACGGA 2 cut(s) 344, 1952
TspMI CCCGGG 2 cut(s) 497, 1744
TspRI CASTG 6 cut(s) 32, 562, 663, 754, 1539, 1744
VpaK11BI GGWCC 8 cut(s) 119, 371, 501, 653, 1019, 1259, 1667, 1748
VspI ATTAAT 1 cut(s) 1176
XapI RAATTY 7 cut(s) 632, 968, 1037, 1487, 1562, 1697, 1868
XceI RCATGY 1 cut(s) 1215
XcmI CCANNNNNNNNNTGG 1 cut(s) 983
XmaI CCCGGG 2 cut(s) 497, 1744
XmiI GTMKAC 1 cut(s) 797
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 6 cut(s) 726, 837, 876, 1463, 1709, 2181
ZrmI AGTACT 1 cut(s) 982
Zsp2I ATGCAT 1 cut(s) 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.