Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0010845

Physical Location & Seq

Genomic Coordinates Reverse (-)

15511801 .. 15516589

Gene Structure 4789 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00012650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGGAGATTTTAGGTGTCGGAATTCAGAAAAGCTATGGAGGTTTGTGGGCTAAATTGGCAATTTTACTGGTTCTGTGTACTATTGAAGGAGCGGTGTGCGATTCGGACTATTTGATTGGGCTCGGAAGCTATGACATCACCGGACCGGCGGCTGATGTCAATATGATGGGGTATGCTAACACGGAGCAGATCGCTTCCGGAGTTCACTTCAGGCTGCGAGCTCGCTCCTTCATTGTGGCGGAGCCGAAGGGCAACCGAGTGGTGTTTGTGAATCTTGATGCTTGCATGGCCTCGCAGCTTGTGAAATTGAAAGTTATTGAGAGGTTGAAGGCAAGGTATGGGGATCTATACACTGAGAAGAATGTCGCTATTAGTGGAATTCACACCCATGCTGGCCCTGGGGGTTATCTACAGTATATTGTGTATATTGTAACATCTCTCGGGTTCGTGCGTCAGTCATTTGATGCCCTTGTTGATGGCATTGAGCAAAGTGTTATTCAAGCCCATGAAAATCTTGCGCCAGGATCAGTTTTTATTAATAAGGGAGAAATCTTAGATGCTGGAGTAAACCGTAGTCCTAGTGCTTATCTCAATAATCCTGCAGCAGAGCGCAGTAAATACAAATATGATGTCGATAAAGAAATGACTCTTCTGAAGTTTGTCGATACTCAATGGGGTCCAGTGGGTAGCTTCAATTGGTTTGCAACTCATGGAACTTCTATGAGTCGGACAAACTCATTAATTAGTGGGGATAACAAGGGTGCTGCTGCACGATTTATGGAAGACTGGTTTGAGGAGAATGGTGGTAGAAACCATGAGTTATTGGAGCTTGCTGCATCCTTTCAGTCTCCTCCTGGTAAGCCAGCTACCAGGTCCTTGAGTGTTGCAAGGCGTGTCAGGGGTGTACTAAGGCAGGCTAACAAGCCTAGATTTGTATCTGCCTTTTGTCAATCAAACTGTGGTGATGTGAGCCCAAATGTTCTAGGAGCTTTCTGCACAGATACTGGGCTACCTTGTGATTTCAATCACAGCACCTGTGGTGGGAAGAATGAGTTGTGCTATGGCCGAGGACCGGGTTACCCAGATGAATTTGAGAGTACGCGTATTATTGGTGAGAGGCAACTCAGAAAAGCGGTGGATCTTTTCAACAAAGCATCTGAACAGTTGACTGGGAAGGTGGTAAAGACATGTCCTGCTGCAATGGGGTTTGGATTTGCTGCTGGAACTACTGACGGACCTGGAGCTTTTGATTTCAAGCAAGGGGATGATAAGGGAAATCCTTTCTGGAAATTGGTGCGCAATGTACTCAAAACACCAGGCCAGGAACAGATTGATTGTCAGAGTCCAAAGCCAATCTTGCTTGATACTGGTGAAATGAAGCAACCATATGACTGGGCGCCTTCAATACTTCCAATTCAGATCTTCCGGATAGGGCAGCTTGTCATTCTCAGTGTACCTGGAGAATTTACAACAATGGCTGGTAGGCGGCTCCGTGATGCTGTGAAAACCGTACTGACTAGTGGTGGTCATGGTGGAAATATTCATGTTGTGCTAGCAGGATTGACTAATACATATTCTCAGTATATCACGACCTTTGAAGAGTATGAGGTGCAGAGATATGAGGGTGCATCCACTCTCTATGGACCACACACGCTAAGTGCCTACATTCAGGAGTTCAAGAAGCTTGCTACAGCTCTCATCAGTAACCAACCTGTCGCATCAGGTCCACAACCCCCAGATCTCCTTGATAAGCAGATAAGCTTGCTTACTCCAGTTGTTATGGATGCAACCCCTGTTGGTGTCAGTTTTGGGGATTGCAGTTCGGATGTGCCTCAAAACTCCACCTTTAAGAGAGGTCATGACATGGTAACAGTTACCTTCTGGTCGGCATGTCCTCGGAATGACCTATTGACGGAAGGTACATTTGCCCTTGTGGAGATTCTCCATGGAAAGGATACTTGGGTTCCAGCTTACGATGACGATGATTTCTGTCTGCGCTTTAAGTGGTCAAGACCTTCCAAACTCAGTACTAGAAGTCAGGCCACCATGGAATGGAGAATTCCTCAGTCCGCAACTCCCGGCGTATACAGAATAAGGCATTTTGGTGCCTCTAAGAGCCTGGTTGGATCGATTCGCCACTTTACAGGTTCATCTAGTGCTTTTGTGGTAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0006950 GO:0006979 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0019725 GO:0031090 GO:0033554 GO:0034599 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050896 GO:0051716 GO:0055082 GO:0055088 GO:0065007 GO:0065008 GO:0070887 GO:0090156 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00600 ko01100 ko04071 map00600 map01100 map04071

Pfam Domains

Protein Families

Ceramidase_alk Ceramidse_alk_C

Protein Analysis

724

Amino Acids

79.03

Weight (kDa)

7.53

Isoelectric Point (pI)

39.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ceramidase_alk	PF04734	37 - 393	9.4e-140	Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidase_alk	PF04734	391 - 557	7e-78	Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C	PF17048	559 - 723	1.6e-54	Neutral/alkaline non-lysosomal ceramidase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010845)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07380 AT1G07380 AT5G58980
fragaria_vesca	FvH4_3g37680
malus_domestica	MD03G1079000.v1.1
prunus_persica	Prupe.6G063600_v2.0.a1
pyrus_communis	pycom03g06300
rosa_chinensis	RchiOBHm_Chr5g0067951
rosa_laevigata	RLG00000035946
rosa_multiflora	Rmu_sc0000435.1_g000039
rosa_roxburghii	Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa	Rorug05G0386600 Rorug05G0386700
rosa_samantha	Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana	Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1298
AccB1I	GGYRCC	2 cut(s)	1396, 2105
AccB7I	CCANNNNNTGG	1 cut(s)	2052
AccBSI	CCGCTC	1 cut(s)	92
AccI	GTMKAC	1 cut(s)	2085
AccII	CGCG	1 cut(s)	1102
AccIII	TCCGGA	2 cut(s)	197, 1425
AciI	CCGC	6 cut(s)	92, 149, 239, 1133, 1486, 2070
AclWI	GGATC	4 cut(s)	351, 532, 1146, 2134
AcoI	YGGCCR	1 cut(s)	1063
AcsI	RAATTY	5 cut(s)	21, 378, 1088, 1463, 2058
AcuI	CTGAAG	2 cut(s)	193, 674
AcyI	GRCGYC	1 cut(s)	1397
AfaI	GTAC	8 cut(s)	79, 906, 1099, 1305, 1455, 1512, 1921, 2029
AfiI	CCNNNNNNNGG	5 cut(s)	1041, 1072, 1912, 1951, 2052
AflIII	ACRYGT	2 cut(s)	1100, 1187
AhlI	ACTAGT	1 cut(s)	1517
AjnI	CCWGG	9 cut(s)	397, 520, 853, 869, 1237, 1315, 1320, 1456, 2118
AjuI	GAANNNNNNNTTGG	2 cut(s)	1942, 1974
Alw21I	GWGCWC	1 cut(s)	223
Alw26I	GTCTC	1 cut(s)	852
AlwI	GGATC	4 cut(s)	351, 532, 1146, 2134
AlwNI	CAGNNNCTG	2 cut(s)	1004, 1035
Ama87I	CYCGRG	1 cut(s)	440
Aor13HI	TCCGGA	2 cut(s)	197, 1425
AoxI	GGCC	5 cut(s)	288, 394, 1063, 1318, 2040
ApeKI	GCWGC	9 cut(s)	214, 295, 602, 764, 767, 833, 1196, 1217, 1435
ApoI	RAATTY	5 cut(s)	21, 378, 1088, 1463, 2058
ArsI	GACNNNNNNTTYG	2 cut(s)	615, 647
AseI	ATTAAT	2 cut(s)	537, 740
AspLEI	GCGC	5 cut(s)	520, 612, 1299, 1399, 1998
AspS9I	GGNCC	8 cut(s)	143, 395, 677, 873, 1070, 1235, 1643, 1724
AsuC2I	CCSGG	2 cut(s)	1074, 2079
AsuHPI	GGTGA	4 cut(s)	130, 974, 1124, 1382
AsuNHI	GCTAGC	1 cut(s)	1552
AvaI	CYCGRG	1 cut(s)	440
AvaII	GGWCC	7 cut(s)	143, 677, 873, 1070, 1235, 1643, 1724
BaeI	ACNNNNGTAYC	2 cut(s)	1947, 1980
BanI	GGYRCC	2 cut(s)	1396, 2105
BanII	GRGCYC	3 cut(s)	123, 223, 974
BbsI	GAAGAC	1 cut(s)	789
Bbv12I	GWGCWC	1 cut(s)	223
BbvI	GCAGC	9 cut(s)	201, 307, 614, 751, 754, 820, 1183, 1204, 1447
BccI	CCATC	2 cut(s)	160, 470
BciT130I	CCWGG	9 cut(s)	399, 522, 855, 871, 1239, 1317, 1322, 1458, 2120
BciVI	GTATCC	1 cut(s)	1948
BcnI	CCSGG	2 cut(s)	1074, 2079
BcoDI	GTCTC	1 cut(s)	852
BcuI	ACTAGT	1 cut(s)	1517
BfaI	CTAG	7 cut(s)	579, 927, 983, 1518, 1553, 2031, 2154
BfmI	CTRYAG	3 cut(s)	410, 600, 1689
BfoI	RGCGCY	1 cut(s)	1400
BfuI	GTATCC	1 cut(s)	1948
BglII	AGATCT	2 cut(s)	1419, 1738
BmcAI	AGTACT	1 cut(s)	2029
Bme18I	GGWCC	7 cut(s)	143, 677, 873, 1070, 1235, 1643, 1724
BmeT110I	CYCGRG	1 cut(s)	440
BmgT120I	GGNCC	8 cut(s)	143, 395, 677, 873, 1070, 1235, 1643, 1724
BmiI	GGNNCC	6 cut(s)	243, 678, 1398, 1490, 1965, 2107
BmrI	ACTGGG	3 cut(s)	1014, 1179, 1402
BmsI	GCATC	9 cut(s)	268, 454, 547, 845, 1163, 1486, 1637, 1727, 1774
BmtI	GCTAGC	1 cut(s)	1556
BmuI	ACTGGG	3 cut(s)	1014, 1179, 1402
BpiI	GAAGAC	1 cut(s)	789
BplI	GAGNNNNNCTC	2 cut(s)	2047, 2079
BpmI	CTGGAG	4 cut(s)	582, 1260, 1479, 1755
BpuEI	CTTGAG	1 cut(s)	898
BpuMI	CCSGG	2 cut(s)	1074, 2079
Bsa29I	ATCGAT	1 cut(s)	2129
BsaBI	GATNNNNATC	2 cut(s)	934, 1023
BsaHI	GRCGYC	1 cut(s)	1397
BsaJI	CCNNGG	6 cut(s)	397, 398, 1066, 1895, 1945, 2046
BsaWI	WCCGGW	3 cut(s)	140, 197, 1425
Bsc4I	CCNNNNNNNGG	5 cut(s)	1041, 1072, 1912, 1951, 2052
Bse118I	RCCGGY	1 cut(s)	145
Bse1I	ACTGG	8 cut(s)	72, 680, 791, 1009, 1174, 1372, 1397, 1772
Bse3DI	GCAATG	2 cut(s)	1206, 1306
Bse8I	GATNNNNATC	2 cut(s)	934, 1023
BseAI	TCCGGA	2 cut(s)	197, 1425
BseBI	CCWGG	9 cut(s)	399, 522, 855, 871, 1239, 1317, 1322, 1458, 2120
BseCI	ATCGAT	1 cut(s)	2129
BseDI	CCNNGG	6 cut(s)	397, 398, 1066, 1895, 1945, 2046
BseGI	GGATG	5 cut(s)	836, 1270, 1628, 1789, 1831
BseJI	GATNNNNATC	2 cut(s)	934, 1023
BseLI	CCNNNNNNNGG	5 cut(s)	1041, 1072, 1912, 1951, 2052
BseMI	GCAATG	2 cut(s)	1206, 1306
BseMII	CTCAG	6 cut(s)	345, 1138, 1462, 1592, 2038, 2078
BseNI	ACTGG	8 cut(s)	72, 680, 791, 1009, 1174, 1372, 1397, 1772
BseRI	GAGGAG	2 cut(s)	809, 840
BseXI	GCAGC	9 cut(s)	201, 307, 614, 751, 754, 820, 1183, 1204, 1447
BsgI	GTGCAG	3 cut(s)	753, 979, 1631
Bsh1236I	CGCG	1 cut(s)	1102
BshFI	GGCC	5 cut(s)	290, 396, 1065, 1320, 2042
BshNI	GGYRCC	2 cut(s)	1396, 2105
BshVI	ATCGAT	1 cut(s)	2129
BsiHKAI	GWGCWC	1 cut(s)	223
BsiHKCI	CYCGRG	1 cut(s)	440
BsiSI	CCGG	6 cut(s)	141, 146, 198, 1073, 1426, 2079
BslI	CCNNNNNNNGG	5 cut(s)	1041, 1072, 1912, 1951, 2052
BsmAI	GTCTC	1 cut(s)	852
BsnI	GGCC	5 cut(s)	290, 396, 1065, 1320, 2042
BsoBI	CYCGRG	1 cut(s)	440
Bsp1286I	GDGCHC	3 cut(s)	123, 223, 974
Bsp13I	TCCGGA	2 cut(s)	197, 1425
Bsp143I	GATC	7 cut(s)	189, 343, 524, 1138, 1419, 1738, 2126
Bsp19I	CCATGG	2 cut(s)	1945, 2046
BspACI	CCGC	6 cut(s)	92, 149, 239, 1133, 1486, 2070
BspANI	GGCC	5 cut(s)	290, 396, 1065, 1320, 2042
BspCNI	CTCAG	6 cut(s)	346, 1137, 1461, 1591, 2037, 2077
BspDI	ATCGAT	1 cut(s)	2129
BspEI	TCCGGA	2 cut(s)	197, 1425
BspFNI	CGCG	1 cut(s)	1102
BspHI	TCATGA	1 cut(s)	1858
BspLI	GGNNCC	6 cut(s)	243, 678, 1398, 1490, 1965, 2107
BspMAI	CTGCAG	1 cut(s)	604
BspOI	GCTAGC	1 cut(s)	1556
BspPI	GGATC	4 cut(s)	351, 532, 1146, 2134
BspT107I	GGYRCC	2 cut(s)	1396, 2105
BsrBI	CCGCTC	1 cut(s)	92
BsrDI	GCAATG	2 cut(s)	1206, 1306
BsrFI	RCCGGY	1 cut(s)	145
BsrI	ACTGG	8 cut(s)	72, 680, 791, 1009, 1174, 1372, 1397, 1772
BssAI	RCCGGY	1 cut(s)	145
BssECI	CCNNGG	6 cut(s)	397, 398, 1066, 1895, 1945, 2046
BssMI	GATC	7 cut(s)	189, 343, 524, 1138, 1419, 1738, 2126
BssNAI	GTATAC	1 cut(s)	2086
BssNI	GRCGYC	1 cut(s)	1397
BssT1I	CCWWGG	2 cut(s)	1945, 2046
Bst1107I	GTATAC	1 cut(s)	2086
Bst2UI	CCWGG	9 cut(s)	399, 522, 855, 871, 1239, 1317, 1322, 1458, 2120
Bst4CI	ACNGT	6 cut(s)	414, 572, 959, 1164, 1510, 1873
Bst6I	CTCTTC	2 cut(s)	654, 1593
BstACI	GRCGYC	1 cut(s)	1397
BstDSI	CCRYGG	2 cut(s)	1945, 2046
BstEII	GGTNACC	1 cut(s)	1076
BstF5I	GGATG	5 cut(s)	836, 1270, 1628, 1789, 1831
BstFNI	CGCG	1 cut(s)	1102
BstH2I	RGCGCY	1 cut(s)	1400
BstHHI	GCGC	5 cut(s)	520, 612, 1299, 1399, 1998
BstKTI	GATC	7 cut(s)	192, 346, 527, 1141, 1422, 1741, 2129
BstMAI	GTCTC	1 cut(s)	852
BstMBI	GATC	7 cut(s)	189, 343, 524, 1138, 1419, 1738, 2126
BstMWI	GCNNNNNNNGC	3 cut(s)	56, 287, 1357
BstNI	CCWGG	9 cut(s)	399, 522, 855, 871, 1239, 1317, 1322, 1458, 2120
BstNSI	RCATGY	2 cut(s)	1191, 1893
BstPI	GGTNACC	1 cut(s)	1076
BstSFI	CTRYAG	3 cut(s)	410, 600, 1689
BstUI	CGCG	1 cut(s)	1102
BstV1I	GCAGC	9 cut(s)	201, 307, 614, 751, 754, 820, 1183, 1204, 1447
BstV2I	GAAGAC	1 cut(s)	789
BstX2I	RGATCY	4 cut(s)	343, 1138, 1419, 1738
BstXI	CCANNNNNNTGG	1 cut(s)	1392
BstYI	RGATCY	4 cut(s)	343, 1138, 1419, 1738
BstZ17I	GTATAC	1 cut(s)	2086
Bsu15I	ATCGAT	1 cut(s)	2129
BsuI	GTATCC	1 cut(s)	1948
BsuRI	GGCC	5 cut(s)	290, 396, 1065, 1320, 2042
BsuTUI	ATCGAT	1 cut(s)	2129
BtgI	CCRYGG	2 cut(s)	1945, 2046
BtsCI	GGATG	5 cut(s)	836, 1270, 1628, 1789, 1831
BtsIMutI	CAGTG	3 cut(s)	351, 687, 1456
CaiI	CAGNNNCTG	2 cut(s)	1004, 1035
CciI	TCATGA	1 cut(s)	1858
CfoI	GCGC	5 cut(s)	520, 612, 1299, 1399, 1998
Cfr10I	RCCGGY	1 cut(s)	145
Cfr13I	GGNCC	8 cut(s)	143, 395, 677, 873, 1070, 1235, 1643, 1724
ClaI	ATCGAT	1 cut(s)	2129
CpoI	CGGWCCG	1 cut(s)	143
CseI	GACGC	1 cut(s)	440
CsiI	ACCWGGT	1 cut(s)	869
Csp6I	GTAC	8 cut(s)	78, 905, 1098, 1304, 1454, 1511, 1920, 2028
CspI	CGGWCCG	1 cut(s)	143
CviQI	GTAC	8 cut(s)	78, 905, 1098, 1304, 1454, 1511, 1920, 2028
DinI	GGCGCC	1 cut(s)	1398
DpnI	GATC	7 cut(s)	191, 345, 526, 1140, 1421, 1740, 2128
DpnII	GATC	7 cut(s)	189, 343, 524, 1138, 1419, 1738, 2126
EaeI	YGGCCR	1 cut(s)	1063
Eam1104I	CTCTTC	2 cut(s)	654, 1593
EarI	CTCTTC	2 cut(s)	654, 1593
EciI	GGCGGA	1 cut(s)	254
Ecl136II	GAGCTC	1 cut(s)	221
Eco130I	CCWWGG	2 cut(s)	1945, 2046
Eco24I	GRGCYC	3 cut(s)	123, 223, 974
Eco47I	GGWCC	7 cut(s)	143, 677, 873, 1070, 1235, 1643, 1724
Eco53kI	GAGCTC	1 cut(s)	221
Eco57I	CTGAAG	2 cut(s)	193, 674
Eco88I	CYCGRG	1 cut(s)	440
Eco91I	GGTNACC	1 cut(s)	1076
EcoICRI	GAGCTC	1 cut(s)	221
EcoO109I	RGGNCCY	1 cut(s)	873
EcoO65I	GGTNACC	1 cut(s)	1076
EcoRI	GAATTC	3 cut(s)	21, 378, 2058
EcoRII	CCWGG	9 cut(s)	397, 520, 853, 869, 1237, 1315, 1320, 1456, 2118
EcoT14I	CCWWGG	2 cut(s)	1945, 2046
EcoT38I	GRGCYC	3 cut(s)	123, 223, 974
EgeI	GGCGCC	1 cut(s)	1398
EheI	GGCGCC	1 cut(s)	1398
ErhI	CCWWGG	2 cut(s)	1945, 2046
FalI	AAGNNNNNCTT	2 cut(s)	1340, 1372
FauNDI	CATATG	1 cut(s)	1387
FblI	GTMKAC	1 cut(s)	2085
FokI	GGATG	5 cut(s)	823, 1277, 1615, 1796, 1838
FriOI	GRGCYC	3 cut(s)	123, 223, 974
FspBI	CTAG	7 cut(s)	579, 927, 983, 1518, 1553, 2031, 2154
FspI	TGCGCA	1 cut(s)	1298
GlaI	GCGC	5 cut(s)	519, 611, 1298, 1398, 1997
GsuI	CTGGAG	4 cut(s)	582, 1260, 1479, 1755
HaeII	RGCGCY	1 cut(s)	1400
HaeIII	GGCC	5 cut(s)	290, 396, 1065, 1320, 2042
HapII	CCGG	6 cut(s)	141, 146, 198, 1073, 1426, 2079
HgaI	GACGC	1 cut(s)	440
HhaI	GCGC	5 cut(s)	520, 612, 1299, 1399, 1998
Hin1I	GRCGYC	1 cut(s)	1397
Hin6I	GCGC	5 cut(s)	518, 610, 1297, 1397, 1996
HinP1I	GCGC	5 cut(s)	518, 610, 1297, 1397, 1996
HincII	GTYRAC	1 cut(s)	1167
HindII	GTYRAC	1 cut(s)	1167
HindIII	AAGCTT	2 cut(s)	1682, 1759
HinfI	GANTC	7 cut(s)	101, 271, 646, 724, 1342, 1939, 2131
HpaII	CCGG	6 cut(s)	141, 146, 198, 1073, 1426, 2079
HphI	GGTGA	4 cut(s)	130, 974, 1124, 1382
Hpy166II	GTNNAC	8 cut(s)	78, 205, 568, 905, 1167, 1454, 1727, 2086
Hpy188III	TCNNGA	9 cut(s)	198, 275, 1285, 1426, 1588, 1670, 1678, 1859, 2010
Hpy8I	GTNNAC	8 cut(s)	78, 205, 568, 905, 1167, 1454, 1727, 2086
HpyAV	CCTTC	9 cut(s)	80, 238, 241, 322, 1168, 1410, 1888, 1910, 2025
HpyCH4III	ACNGT	6 cut(s)	414, 572, 959, 1164, 1510, 1873
HpyF10VI	GCNNNNNNNGC	3 cut(s)	56, 287, 1357
Hsp92I	GRCGYC	1 cut(s)	1397
HspAI	GCGC	5 cut(s)	518, 610, 1297, 1397, 1996
KasI	GGCGCC	1 cut(s)	1396
Kpn2I	TCCGGA	2 cut(s)	197, 1425
Kzo9I	GATC	7 cut(s)	189, 343, 524, 1138, 1419, 1738, 2126
LmnI	GCTCC	8 cut(s)	89, 184, 230, 241, 826, 986, 1241, 1494
Lsp1109I	GCAGC	9 cut(s)	201, 307, 614, 751, 754, 820, 1183, 1204, 1447
LweI	GCATC	9 cut(s)	268, 454, 547, 845, 1163, 1486, 1637, 1727, 1774
MabI	ACCWGGT	1 cut(s)	869
MaeI	CTAG	7 cut(s)	579, 927, 983, 1518, 1553, 2031, 2154
MaeIII	GTNAC	5 cut(s)	430, 1076, 1703, 1867, 1873
MalI	GATC	7 cut(s)	191, 345, 526, 1140, 1421, 1740, 2128
MbiI	CCGCTC	1 cut(s)	92
MboI	GATC	7 cut(s)	189, 343, 524, 1138, 1419, 1738, 2126
MboII	GAAGA	6 cut(s)	370, 641, 794, 1057, 1414, 1610
MfeI	CAATTG	1 cut(s)	694
MflI	RGATCY	4 cut(s)	343, 1138, 1419, 1738
MhlI	GDGCHC	3 cut(s)	123, 223, 974
MluI	ACGCGT	1 cut(s)	1100
Mly113I	GGCGCC	1 cut(s)	1397
MlyI	GAGTC	3 cut(s)	640, 733, 1351
MmeI	TCCRAC	2 cut(s)	707, 2104
MroI	TCCGGA	2 cut(s)	197, 1425
MseI	TTAA	4 cut(s)	537, 740, 1848, 2001
MslI	CAYNNNNRTG	2 cut(s)	387, 2103
MspI	CCGG	6 cut(s)	141, 146, 198, 1073, 1426, 2079
MunI	CAATTG	1 cut(s)	694
MvaI	CCWGG	9 cut(s)	399, 522, 855, 871, 1239, 1317, 1322, 1458, 2120
MvnI	CGCG	1 cut(s)	1102
MwoI	GCNNNNNNNGC	3 cut(s)	56, 287, 1357
NarI	GGCGCC	1 cut(s)	1397
NciI	CCSGG	2 cut(s)	1074, 2079
NcoI	CCATGG	2 cut(s)	1945, 2046
NdeI	CATATG	1 cut(s)	1387
NdeII	GATC	7 cut(s)	189, 343, 524, 1138, 1419, 1738, 2126
NheI	GCTAGC	1 cut(s)	1552
NlaIV	GGNNCC	6 cut(s)	243, 678, 1398, 1490, 1965, 2107
NmeAIII	GCCGAG	1 cut(s)	1091
NsbI	TGCGCA	1 cut(s)	1298
NspI	RCATGY	2 cut(s)	1191, 1893
PagI	TCATGA	1 cut(s)	1858
PasI	CCCWGGG	1 cut(s)	398
PciI	ACATGT	1 cut(s)	1187
PfeI	GAWTC	4 cut(s)	101, 271, 1939, 2131
PflMI	CCANNNNNTGG	1 cut(s)	2052
PleI	GAGTC	3 cut(s)	640, 732, 1350
PluTI	GGCGCC	1 cut(s)	1400
PpsI	GAGTC	3 cut(s)	640, 732, 1350
PpuMI	RGGWCCY	1 cut(s)	873
PscI	ACATGT	1 cut(s)	1187
PshBI	ATTAAT	2 cut(s)	537, 740
Psp124BI	GAGCTC	1 cut(s)	223
Psp5II	RGGWCCY	1 cut(s)	873
Psp6I	CCWGG	9 cut(s)	397, 520, 853, 869, 1237, 1315, 1320, 1456, 2118
PspEI	GGTNACC	1 cut(s)	1076
PspGI	CCWGG	9 cut(s)	397, 520, 853, 869, 1237, 1315, 1320, 1456, 2118
PspN4I	GGNNCC	6 cut(s)	243, 678, 1398, 1490, 1965, 2107
PspPI	GGNCC	8 cut(s)	143, 395, 677, 873, 1070, 1235, 1643, 1724
PspPPI	RGGWCCY	1 cut(s)	873
PstI	CTGCAG	1 cut(s)	604
PstNI	CAGNNNCTG	2 cut(s)	1004, 1035
PsuI	RGATCY	4 cut(s)	343, 1138, 1419, 1738
RsaI	GTAC	8 cut(s)	79, 906, 1099, 1305, 1455, 1512, 1921, 2029
RsaNI	GTAC	8 cut(s)	78, 905, 1098, 1304, 1454, 1511, 1920, 2028
RseI	CAYNNNNRTG	2 cut(s)	387, 2103
Rsr2I	CGGWCCG	1 cut(s)	143
RsrII	CGGWCCG	1 cut(s)	143
SacI	GAGCTC	1 cut(s)	223
SaqAI	TTAA	4 cut(s)	537, 740, 1848, 2001
Sau3AI	GATC	7 cut(s)	189, 343, 524, 1138, 1419, 1738, 2126
Sau96I	GGNCC	8 cut(s)	143, 395, 677, 873, 1070, 1235, 1643, 1724
ScaI	AGTACT	1 cut(s)	2029
SchI	GAGTC	3 cut(s)	640, 733, 1351
SduI	GDGCHC	3 cut(s)	123, 223, 974
SexAI	ACCWGGT	1 cut(s)	869
SfaNI	GCATC	9 cut(s)	268, 454, 547, 845, 1163, 1486, 1637, 1727, 1774
SfcI	CTRYAG	3 cut(s)	410, 600, 1689
SfoI	GGCGCC	1 cut(s)	1398
SinI	GGWCC	7 cut(s)	143, 677, 873, 1070, 1235, 1643, 1724
SmiMI	CAYNNNNRTG	2 cut(s)	387, 2103
SmlI	CTYRAG	1 cut(s)	877
SmoI	CTYRAG	1 cut(s)	877
SpeI	ACTAGT	1 cut(s)	1517
SsiI	CCGC	6 cut(s)	92, 149, 239, 1133, 1486, 2070
SspDI	GGCGCC	1 cut(s)	1396
SspI	AATATT	1 cut(s)	1540
SspMI	CTAG	7 cut(s)	579, 927, 983, 1518, 1553, 2031, 2154
SstI	GAGCTC	1 cut(s)	223
StyI	CCWWGG	2 cut(s)	1945, 2046
TaaI	ACNGT	6 cut(s)	414, 572, 959, 1164, 1510, 1873
TaqI	TCGA	3 cut(s)	633, 663, 2129
TatI	WGTACW	4 cut(s)	77, 904, 1303, 2027
TauI	GCSGC	2 cut(s)	152, 1489
TfiI	GAWTC	4 cut(s)	101, 271, 1939, 2131
Tru1I	TTAA	4 cut(s)	537, 740, 1848, 2001
Tru9I	TTAA	4 cut(s)	537, 740, 1848, 2001
TscAI	CASTG	3 cut(s)	358, 687, 1456
TseI	GCWGC	9 cut(s)	214, 295, 602, 764, 767, 833, 1196, 1217, 1435
TspDTI	ATGAA	6 cut(s)	220, 522, 1101, 1391, 1532, 2139
TspGWI	ACGGA	4 cut(s)	197, 1248, 1481, 1928
TspRI	CASTG	3 cut(s)	358, 687, 1456
Van91I	CCANNNNNTGG	1 cut(s)	2052
VpaK11BI	GGWCC	7 cut(s)	143, 677, 873, 1070, 1235, 1643, 1724
VspI	ATTAAT	2 cut(s)	537, 740
XapI	RAATTY	5 cut(s)	21, 378, 1088, 1463, 2058
XceI	RCATGY	2 cut(s)	1191, 1893
XcmI	CCANNNNNNNNNTGG	1 cut(s)	395
XmiI	GTMKAC	1 cut(s)	2085
XspI	CTAG	7 cut(s)	579, 927, 983, 1518, 1553, 2031, 2154
ZrmI	AGTACT	1 cut(s)	2029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.