Rmu_sc0000435.1_g000039

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000435.1
OG0010845
Physical Location & Seq
Reverse (-)
171675 .. 182094
10420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000435.1_g000039.1.cds

Sequence Viewer

Length: 3876 bp
atggagtttttaggtgtcggaattcggaaaagctatggaggtttgtgggctaaactggcaattttactgtttctgtgtactattgaacgagcggtgtgcgattccgactatttgattgggctcgggagctatgacatcaccggaccggcggctgatgtcaatatgatggggtatgctaacacggagcagatcgctgccggagttcatttcaggctgcgagctcgctcgttcattgtggcggagccgaagggcaaccgagtggtgtttgtgaatcttgatgcttgcatggcctcgcagcttgtgaaattgaaagtgattgagaggttgaaggcaaggtatggggatctatacactgagaagaatgtagctattagtggaattcacacccatgctggccctgggggttatctacagtatattgtgtatattgtaacatctctcgggtttgtgcgtcagtcatttgatgcccttgttgatggcattgagcaaagcgttattcaagcccatgaaaatcttgcgccaggatcagtttttgttaataagggagaaatcttagatgctggagtaaaccgtagtcctagtgcttatctcaataatcctgcagcagagcgcagtaaatacaaatatgatgtcgataaagaaatgactcttctgaagtttgtcgataatcaatggggtccagtgggtagcttcaattggtttgcaactcatggaacttctatgagtcggacaaactcattaattagtggggataacaagggtgctgctgcacgatttatggaagactggtttgaggagaatggtggtagaagtgcatattctgatgatattgatgctgccgatataccgcgaagagtgtcaaacgtagtttctgaccatcatgataatcaccatgagttactggagcttgctgcatcctttcagtctcctcctggtaagccagcaaccaggtccttgagtgttgcaagacgtgtcaggggtgtactaaggcaggctaacaagcctagatttgtttctgccttttgtcaatcaaactgtggtgatgtgagcccaaatgttctaggagctttctgcacagatactgggctaccttgtgatttcaatcacagcacctgtggtgggaagaatgagttgtgctatggccgaggaccgggttacccagatgaatttgaaagtacacgtattattgctgagaggcaactgagaaaagctgtggatcttttcaacaaagcatctgaacagttgaccgggaagattgaatatcgccacacttacctcaacttctcccagcttgaagtaacccttcccaagaaggggggaggttctgaggtggtaaagacatgtcctgctgcaatgggatttggatttgctgctggaaccactgatggacctggagcttttgatttcaagcaaggggatgataagggaaatcctttctggagattagtgcgcaatgtactcaaaacaccaggccaggaacaaattgattgtcagagtccaaagccaatcttgcttgatactggtgaaatgaagcaaccatatgactgggcgccttcaatacttccaattcagatcttccggatagggcagcttgtcattctcagtgtacctggagaatttacaacaatggctggtaggcggctccgtgatgctgtgaaaaccgtactgactagtggtggtcatggtggaaatattcacgttgtgctagcaggattgactaatacttattctcagtatattacgacctttgaagagtacgaagtgcagagatatgagggtgcatccactctctatggaccacacacgctaagtgcctacattcaggagttcaagaagcttgctacagctctcatcagtaaccaacctgtcgcatcaggtccacaacccccagatctccttgataagcagataagcttgcttactccagttgttatggatggaacccctgttggtgtcagttttggggattgcagttcggatgtgcctcaaaactccacctttaagagaggtcatgacatggtaacagttaccttctggtcggcttgtcctcggaatgacctattgacggaaggtacatttgcccttgtggagattctccatggaaaggatacttgggttccagcttacgatgacgatgatttctgtctgcgctttaagtggtcaagaccttccaaactcagtactagaagtcaggcgaccatggaatggagaattcctcagtccgcaactcccggcgtatacagaataaggcattttggtgcctctaagagcctggttggatcgattcgccactttacggctgagctccgactgcttcagactttccgacacggagcgaagaatattcttaaaagaatattcgagaagccaggcaggcttgagttaggagagttcaaactgaaaacgaagaagaagaagaaagaaagaaagaagaaaatgtcggtccaagagtatctggataatcacttgctctctcgcaaaatcgaagacgccgtcaatgccgccgtcagggccaaagccgccgatcccgtcctcttcatctcgaatcatatgaggaaggcgattccctcagtgataacgaaggtcaaagctcggcagatcctcgatagcagaggaattcctactgttgaagttgatcttcatactaacaagggcatgtttcgtgcttcagctccgagttccgatcttactggatttggtgaaactaggtatgaggccgttgagttgcgagatggggataagggatcgtatctcggaagtagtgtgaataaagctgttaagaatgtgaatgagaagatatgtgaagcattggttgggatggatcctacgcttcaatcgcagattgatcaggcaatgatagacttggataagacagaaaagaagggtgaacttggagtgaatgccatattggctgtgtcgatggctgcttgcaaagctggggctgctcagaaagaggttccactgtacaaacacattgctgacctttctggaagaaacaccctgaccctgcctgttcctgctttcactcttataagtggaggaaagcgtgctgggaatagtctagccattcaggaaattatgattctcccaattggagctaacaggtttgcggaggcactacaaatgggatcagaaacttatcatcacttgaaggctgttattgtagacaagtatggcgcacatggatgtaatgtcggtgaagatggtggttttgctcccaacatctccagttttagagaaggcttggatcttgtaaaagaggctatcagcagaacaggctataatgaccggataaaaatagcaattgatgttgctgctacgaacttctgcatgggtacaaagtatgacctggactacaaatcaccgaataagtcagggcaaaactttaagtcggcagaagatatgattaagttgtatagagaactttgtactgagtacccaattgtatcaattgaagatccgtttgataaggaggcctgggaacacaccaaacggttttctaatcttgcaatttgtcaggtagttggagatgacttgttaatgtcaaattcacaacgtatgaagaaagcaatagaggaatctacgtgtgatgctcttcttctgaagattaaccagattgggacagtgactgaagccattgaagtcgttaaactggcaaaggatgcccactggggagttgtggcatctcatagatgtggtgaaactgaagagtcctttatagctgacttctctgttggtctctctactggtcaaatcaaagctggtgctccttgcagaagcgagcgactggccaagtataaccagttgcttcgaattgaggaagagcttggggacgatgcattttatgccggtgaagagtggagggaatcaccctga

Protein Analysis

1291

Amino Acids

142.09

Weight (kDa)

7.71

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3032
Acc16I TGCGCA 1 cut(s) 1451
AccB1I GGYRCC 2 cut(s) 1549, 2258
AccB7I CCANNNNNTGG 1 cut(s) 2205
AccBSI CCGCTC 1 cut(s) 92
AccI GTMKAC 2 cut(s) 2238, 3165
AccII CGCG 1 cut(s) 850
AccIII TCCGGA 1 cut(s) 1578
AciI CCGC 9 cut(s) 92, 149, 239, 848, 1639, 2223, 2502, 2520, 3110
AcoI YGGCCR 2 cut(s) 1141, 3789
AcsI RAATTY 7 cut(s) 21, 378, 1166, 1616, 2211, 2616, 3547
AcuI CTGAAG 6 cut(s) 674, 2300, 2652, 3623, 3651, 3726
AcyI GRCGYC 2 cut(s) 1550, 2490
AdeI CACNNNGTG 1 cut(s) 1702
AfiI CCNNNNNNNGG 9 cut(s) 1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296, 2508
AflIII ACRYGT 4 cut(s) 970, 1178, 1340, 3584
AhlI ACTAGT 1 cut(s) 1670
AjiI CACGTC 1 cut(s) 971
AjuI GAANNNNNNNTTGG 6 cut(s) 2095, 2127, 2796, 2828, 2891, 2923
Alw21I GWGCWC 3 cut(s) 223, 2307, 3769
Alw26I GTCTC 2 cut(s) 930, 3743
AlwNI CAGNNNCTG 3 cut(s) 1082, 1113, 3629
Ama87I CYCGRG 2 cut(s) 122, 440
Aor13HI TCCGGA 1 cut(s) 1578
AoxI GGCC 8 cut(s) 288, 394, 1141, 1471, 2511, 2715, 3474, 3789
ApoI RAATTY 7 cut(s) 21, 378, 1166, 1616, 2211, 2616, 3547
ArsI GACNNNNNNTTYG 6 cut(s) 615, 647, 1474, 1506, 2478, 2510
AseI ATTAAT 1 cut(s) 740
AspLEI GCGC 6 cut(s) 520, 612, 1452, 1552, 2151, 3179
AsuC2I CCSGG 3 cut(s) 1152, 1249, 2232
AsuII TTCGAA 1 cut(s) 3811
AsuNHI GCTAGC 1 cut(s) 1705
AvaI CYCGRG 2 cut(s) 122, 440
AvaII GGWCC 8 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877, 2443
BaeI ACNNNNGTAYC 2 cut(s) 2100, 2133
BalI TGGCCA 1 cut(s) 3791
BamHI GGATCC 1 cut(s) 2821
BanI GGYRCC 2 cut(s) 1549, 2258
BanII GRGCYC 4 cut(s) 123, 223, 1052, 2307
BbsI GAAGAC 2 cut(s) 789, 2493
Bbv12I GWGCWC 3 cut(s) 223, 2307, 3769
BccI CCATC 9 cut(s) 160, 470, 885, 1379, 1931, 2726, 2812, 2914, 3197
BceAI ACGGC 4 cut(s) 2313, 2477, 2489, 2702
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciVI GTATCC 1 cut(s) 2101
BclI TGATCA 1 cut(s) 2845
BcnI CCSGG 3 cut(s) 1152, 1249, 2232
BcoDI GTCTC 2 cut(s) 930, 3743
BcuI ACTAGT 1 cut(s) 1670
BfaI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
BfmI CTRYAG 3 cut(s) 410, 600, 1842
BfoI RGCGCY 1 cut(s) 1553
BfuI GTATCC 1 cut(s) 2101
BglI GCCNNNNNGGC 3 cut(s) 2374, 2510, 2909
BglII AGATCT 2 cut(s) 1572, 1891
BlpI GCTNAGC 1 cut(s) 2301
BmcAI AGTACT 1 cut(s) 2182
Bme18I GGWCC 8 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877, 2443
BmeT110I CYCGRG 2 cut(s) 122, 440
BmgBI CACGTC 1 cut(s) 971
BmrI ACTGGG 3 cut(s) 1092, 1555, 3679
BmtI GCTAGC 1 cut(s) 1709
BmuI ACTGGG 3 cut(s) 1092, 1555, 3679
BpiI GAAGAC 2 cut(s) 789, 2493
BplI GAGNNNNNCTC 2 cut(s) 2200, 2232
BpmI CTGGAG 7 cut(s) 582, 923, 1413, 1459, 1632, 1908, 3211
Bpu1102I GCTNAGC 1 cut(s) 2301
Bpu14I TTCGAA 1 cut(s) 3811
BpuEI CTTGAG 2 cut(s) 976, 2399
BpuMI CCSGG 3 cut(s) 1152, 1249, 2232
Bsa29I ATCGAT 1 cut(s) 2282
BsaAI YACGTR 2 cut(s) 1181, 3585
BsaBI GATNNNNATC 1 cut(s) 1101
BsaHI GRCGYC 2 cut(s) 1550, 2490
BsaI GGTCTC 1 cut(s) 3743
BsaJI CCNNGG 7 cut(s) 397, 398, 1144, 2048, 2098, 2199, 3477
BsaWI WCCGGW 3 cut(s) 140, 1578, 3288
Bsc4I CCNNNNNNNGG 9 cut(s) 1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296, 2508
Bse118I RCCGGY 2 cut(s) 145, 3848
Bse3DI GCAATG 4 cut(s) 1359, 1459, 2859, 2973
Bse8I GATNNNNATC 1 cut(s) 1101
BseAI TCCGGA 1 cut(s) 1578
BseCI ATCGAT 1 cut(s) 2282
BseDI CCNNGG 7 cut(s) 397, 398, 1144, 2048, 2098, 2199, 3477
BseGI GGATG 8 cut(s) 914, 1423, 1781, 1942, 1984, 2823, 3191, 3667
BseJI GATNNNNATC 1 cut(s) 1101
BseLI CCNNNNNNNGG 9 cut(s) 1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296, 2508
BseMI GCAATG 4 cut(s) 1359, 1459, 2859, 2973
BseRI GAGGAG 2 cut(s) 809, 918
BseYI CCCAGC 3 cut(s) 1287, 2936, 3050
BsgI GTGCAG 3 cut(s) 753, 1057, 1784
Bsh1236I CGCG 1 cut(s) 850
BshFI GGCC 8 cut(s) 290, 396, 1143, 1473, 2513, 2717, 3476, 3791
BshNI GGYRCC 2 cut(s) 1549, 2258
BshVI ATCGAT 1 cut(s) 2282
BsiHKAI GWGCWC 3 cut(s) 223, 2307, 3769
BsiHKCI CYCGRG 2 cut(s) 122, 440
BsiSI CCGG 9 cut(s) 141, 146, 198, 1151, 1248, 1579, 2232, 3289, 3849
BslFI GGGAC 2 cut(s) 3634, 3845
BslI CCNNNNNNNGG 9 cut(s) 1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296, 2508
BsmAI GTCTC 2 cut(s) 930, 3743
BsmFI GGGAC 2 cut(s) 3634, 3845
BsmI GAATGC 1 cut(s) 2905
BsnI GGCC 8 cut(s) 290, 396, 1143, 1473, 2513, 2717, 3476, 3791
Bso31I GGTCTC 1 cut(s) 3743
BsoBI CYCGRG 2 cut(s) 122, 440
Bsp119I TTCGAA 1 cut(s) 3811
Bsp1286I GDGCHC 5 cut(s) 123, 223, 1052, 2307, 3769
Bsp13I TCCGGA 1 cut(s) 1578
Bsp1407I TGTACA 1 cut(s) 2964
Bsp1720I GCTNAGC 1 cut(s) 2301
Bsp19I CCATGG 2 cut(s) 2098, 2199
BspACI CCGC 9 cut(s) 92, 149, 239, 848, 1639, 2223, 2502, 2520, 3110
BspANI GGCC 8 cut(s) 290, 396, 1143, 1473, 2513, 2717, 3476, 3791
BspDI ATCGAT 1 cut(s) 2282
BspEI TCCGGA 1 cut(s) 1578
BspFNI CGCG 1 cut(s) 850
BspHI TCATGA 2 cut(s) 880, 2011
BspMAI CTGCAG 1 cut(s) 604
BspOI GCTAGC 1 cut(s) 1709
BspQI GCTCTTC 2 cut(s) 3600, 3816
BspT104I TTCGAA 1 cut(s) 3811
BspT107I GGYRCC 2 cut(s) 1549, 2258
BspTNI GGTCTC 1 cut(s) 3743
BsrBI CCGCTC 1 cut(s) 92
BsrDI GCAATG 4 cut(s) 1359, 1459, 2859, 2973
BsrFI RCCGGY 2 cut(s) 145, 3848
BsrGI TGTACA 1 cut(s) 2964
BssAI RCCGGY 2 cut(s) 145, 3848
BssECI CCNNGG 7 cut(s) 397, 398, 1144, 2048, 2098, 2199, 3477
BssNAI GTATAC 1 cut(s) 2239
BssNI GRCGYC 2 cut(s) 1550, 2490
BssT1I CCWWGG 2 cut(s) 2098, 2199
Bst1107I GTATAC 1 cut(s) 2239
Bst6I CTCTTC 8 cut(s) 654, 847, 1746, 2540, 3600, 3702, 3816, 3849
BstACI GRCGYC 2 cut(s) 1550, 2490
BstAPI GCANNNNNTGC 2 cut(s) 3662, 3845
BstAUI TGTACA 1 cut(s) 2964
BstBAI YACGTR 2 cut(s) 1181, 3585
BstBI TTCGAA 1 cut(s) 3811
BstDSI CCRYGG 2 cut(s) 2098, 2199
BstEII GGTNACC 1 cut(s) 1154
BstF5I GGATG 8 cut(s) 914, 1423, 1781, 1942, 1984, 2823, 3191, 3667
BstFNI CGCG 1 cut(s) 850
BstH2I RGCGCY 1 cut(s) 1553
BstHHI GCGC 6 cut(s) 520, 612, 1452, 1552, 2151, 3179
BstMAI GTCTC 2 cut(s) 930, 3743
BstNSI RCATGY 2 cut(s) 1344, 2659
BstPI GGTNACC 1 cut(s) 1154
BstSFI CTRYAG 3 cut(s) 410, 600, 1842
BstUI CGCG 1 cut(s) 850
BstV2I GAAGAC 2 cut(s) 789, 2493
BstX2I RGATCY 8 cut(s) 343, 1216, 1572, 1891, 2598, 2821, 3247, 3457
BstXI CCANNNNNNTGG 1 cut(s) 1545
BstYI RGATCY 8 cut(s) 343, 1216, 1572, 1891, 2598, 2821, 3247, 3457
BstZ17I GTATAC 1 cut(s) 2239
Bsu15I ATCGAT 1 cut(s) 2282
BsuI GTATCC 1 cut(s) 2101
BsuRI GGCC 8 cut(s) 290, 396, 1143, 1473, 2513, 2717, 3476, 3791
BsuTUI ATCGAT 1 cut(s) 2282
BtgI CCRYGG 2 cut(s) 2098, 2199
BtrI CACGTC 1 cut(s) 971
BtsCI GGATG 8 cut(s) 914, 1423, 1781, 1942, 1984, 2823, 3191, 3667
BtsIMutI CAGTG 8 cut(s) 351, 687, 1380, 1609, 2577, 2960, 3630, 3667
CaiI CAGNNNCTG 3 cut(s) 1082, 1113, 3629
CciI TCATGA 2 cut(s) 880, 2011
CfoI GCGC 6 cut(s) 520, 612, 1452, 1552, 2151, 3179
Cfr10I RCCGGY 2 cut(s) 145, 3848
ClaI ATCGAT 1 cut(s) 2282
CpoI CGGWCCG 1 cut(s) 143
CseI GACGC 2 cut(s) 440, 2498
CsiI ACCWGGT 1 cut(s) 947
CspI CGGWCCG 1 cut(s) 143
DinI GGCGCC 1 cut(s) 1551
DraIII CACNNNGTG 1 cut(s) 1702
EaeI YGGCCR 2 cut(s) 1141, 3789
Eam1104I CTCTTC 8 cut(s) 654, 847, 1746, 2540, 3600, 3702, 3816, 3849
EarI CTCTTC 8 cut(s) 654, 847, 1746, 2540, 3600, 3702, 3816, 3849
EciI GGCGGA 1 cut(s) 254
Ecl136II GAGCTC 2 cut(s) 221, 2305
Eco130I CCWWGG 2 cut(s) 2098, 2199
Eco147I AGGCCT 1 cut(s) 3476
Eco24I GRGCYC 4 cut(s) 123, 223, 1052, 2307
Eco31I GGTCTC 1 cut(s) 3743
Eco47I GGWCC 8 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877, 2443
Eco53kI GAGCTC 2 cut(s) 221, 2305
Eco57I CTGAAG 6 cut(s) 674, 2300, 2652, 3623, 3651, 3726
Eco88I CYCGRG 2 cut(s) 122, 440
Eco91I GGTNACC 1 cut(s) 1154
EcoICRI GAGCTC 2 cut(s) 221, 2305
EcoO109I RGGNCCY 1 cut(s) 951
EcoO65I GGTNACC 1 cut(s) 1154
EcoRI GAATTC 4 cut(s) 21, 378, 2211, 2616
EcoT14I CCWWGG 2 cut(s) 2098, 2199
EcoT22I ATGCAT 1 cut(s) 3841
EcoT38I GRGCYC 4 cut(s) 123, 223, 1052, 2307
EgeI GGCGCC 1 cut(s) 1551
EheI GGCGCC 1 cut(s) 1551
ErhI CCWWGG 2 cut(s) 2098, 2199
FalI AAGNNNNNCTT 6 cut(s) 1287, 1319, 1493, 1525, 2622, 2654
FaqI GGGAC 2 cut(s) 3634, 3845
FauNDI CATATG 2 cut(s) 1540, 2550
FbaI TGATCA 1 cut(s) 2845
FblI GTMKAC 2 cut(s) 2238, 3165
FokI GGATG 8 cut(s) 901, 1430, 1768, 1949, 1991, 2830, 3198, 3674
FriOI GRGCYC 4 cut(s) 123, 223, 1052, 2307
FspBI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
FspI TGCGCA 1 cut(s) 1451
GlaI GCGC 6 cut(s) 519, 611, 1451, 1551, 2150, 3178
GsaI CCCAGC 3 cut(s) 1291, 2940, 3054
GsuI CTGGAG 7 cut(s) 582, 923, 1413, 1459, 1632, 1908, 3211
HaeII RGCGCY 1 cut(s) 1553
HaeIII GGCC 8 cut(s) 290, 396, 1143, 1473, 2513, 2717, 3476, 3791
HapII CCGG 9 cut(s) 141, 146, 198, 1151, 1248, 1579, 2232, 3289, 3849
HgaI GACGC 2 cut(s) 440, 2498
HhaI GCGC 6 cut(s) 520, 612, 1452, 1552, 2151, 3179
Hin1I GRCGYC 2 cut(s) 1550, 2490
Hin6I GCGC 6 cut(s) 518, 610, 1450, 1550, 2149, 3177
HinP1I GCGC 6 cut(s) 518, 610, 1450, 1550, 2149, 3177
HincII GTYRAC 1 cut(s) 1245
HindII GTYRAC 1 cut(s) 1245
HindIII AAGCTT 2 cut(s) 1835, 1912
HpaII CCGG 9 cut(s) 141, 146, 198, 1151, 1248, 1579, 2232, 3289, 3849
HpyCH4IV ACGT 6 cut(s) 864, 970, 1180, 1698, 3556, 3584
HpySE526I ACGT 6 cut(s) 864, 970, 1180, 1698, 3556, 3584
Hsp92I GRCGYC 2 cut(s) 1550, 2490
HspAI GCGC 6 cut(s) 518, 610, 1450, 1550, 2149, 3177
KasI GGCGCC 1 cut(s) 1549
Kpn2I TCCGGA 1 cut(s) 1578
Ksp22I TGATCA 1 cut(s) 2845
LguI GCTCTTC 2 cut(s) 3600, 3816
MabI ACCWGGT 1 cut(s) 947
MaeI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
MaeII ACGT 6 cut(s) 864, 970, 1180, 1698, 3556, 3584
MaeIII GTNAC 8 cut(s) 430, 897, 1154, 1297, 1856, 2020, 2026, 3625
MbiI CCGCTC 1 cut(s) 92
MfeI CAATTG 5 cut(s) 694, 3090, 3303, 3441, 3450
MflI RGATCY 8 cut(s) 343, 1216, 1572, 1891, 2598, 2821, 3247, 3457
MhlI GDGCHC 5 cut(s) 123, 223, 1052, 2307, 3769
MlsI TGGCCA 1 cut(s) 3791
MluNI TGGCCA 1 cut(s) 3791
Mly113I GGCGCC 1 cut(s) 1550
MlyI GAGTC 4 cut(s) 640, 733, 1504, 3719
MmeI TCCRAC 6 cut(s) 129, 707, 2257, 2332, 2350, 3505
Mox20I TGGCCA 1 cut(s) 3791
Mph1103I ATGCAT 1 cut(s) 3841
MroI TCCGGA 1 cut(s) 1578
MscI TGGCCA 1 cut(s) 3791
MslI CAYNNNNRTG 4 cut(s) 387, 2256, 3184, 3693
Msp20I TGGCCA 1 cut(s) 3791
MspI CCGG 9 cut(s) 141, 146, 198, 1151, 1248, 1579, 2232, 3289, 3849
MunI CAATTG 5 cut(s) 694, 3090, 3303, 3441, 3450
Mva1269I GAATGC 1 cut(s) 2905
MvnI CGCG 1 cut(s) 850
NarI GGCGCC 1 cut(s) 1550
NciI CCSGG 3 cut(s) 1152, 1249, 2232
NcoI CCATGG 2 cut(s) 2098, 2199
NdeI CATATG 2 cut(s) 1540, 2550
NheI GCTAGC 1 cut(s) 1705
NmeAIII GCCGAG 2 cut(s) 1169, 2572
NmuCI GTSAC 1 cut(s) 3625
NsbI TGCGCA 1 cut(s) 1451
NsiI ATGCAT 1 cut(s) 3841
NspI RCATGY 2 cut(s) 1344, 2659
NspV TTCGAA 1 cut(s) 3811
PagI TCATGA 2 cut(s) 880, 2011
PasI CCCWGGG 1 cut(s) 398
PceI AGGCCT 1 cut(s) 3476
PciI ACATGT 1 cut(s) 1340
PciSI GCTCTTC 2 cut(s) 3600, 3816
PcsI WCGNNNNNNNCGW 1 cut(s) 2490
PctI GAATGC 1 cut(s) 2905
PfeI GAWTC 9 cut(s) 101, 271, 2092, 2284, 2545, 2563, 3082, 3578, 3866
PflFI GACNNNGTC 1 cut(s) 2492
PflMI CCANNNNNTGG 1 cut(s) 2205
PleI GAGTC 4 cut(s) 640, 732, 1503, 3718
PluTI GGCGCC 1 cut(s) 1553
PpsI GAGTC 4 cut(s) 640, 732, 1503, 3718
Ppu21I YACGTR 2 cut(s) 1181, 3585
PpuMI RGGWCCY 1 cut(s) 951
PscI ACATGT 1 cut(s) 1340
PshBI ATTAAT 1 cut(s) 740
PsiI TTATAA 1 cut(s) 3032
Psp124BI GAGCTC 2 cut(s) 223, 2307
Psp5II RGGWCCY 1 cut(s) 951
PspEI GGTNACC 1 cut(s) 1154
PspFI CCCAGC 3 cut(s) 1287, 2936, 3050
PspPPI RGGWCCY 1 cut(s) 951
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 3 cut(s) 1082, 1113, 3629
PsuI RGATCY 8 cut(s) 343, 1216, 1572, 1891, 2598, 2821, 3247, 3457
PsyI GACNNNGTC 1 cut(s) 2492
RseI CAYNNNNRTG 4 cut(s) 387, 2256, 3184, 3693
Rsr2I CGGWCCG 1 cut(s) 143
RsrII CGGWCCG 1 cut(s) 143
SacI GAGCTC 2 cut(s) 223, 2307
SapI GCTCTTC 2 cut(s) 3600, 3816
ScaI AGTACT 1 cut(s) 2182
SchI GAGTC 4 cut(s) 640, 733, 1504, 3719
SduI GDGCHC 5 cut(s) 123, 223, 1052, 2307, 3769
SexAI ACCWGGT 1 cut(s) 947
SfcI CTRYAG 3 cut(s) 410, 600, 1842
SfoI GGCGCC 1 cut(s) 1551
SfuI TTCGAA 1 cut(s) 3811
SinI GGWCC 8 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877, 2443
SmiMI CAYNNNNRTG 4 cut(s) 387, 2256, 3184, 3693
SmlI CTYRAG 2 cut(s) 955, 2378
SmoI CTYRAG 2 cut(s) 955, 2378
SpeI ACTAGT 1 cut(s) 1670
SseBI AGGCCT 1 cut(s) 3476
SsiI CCGC 9 cut(s) 92, 149, 239, 848, 1639, 2223, 2502, 2520, 3110
SspDI GGCGCC 1 cut(s) 1549
SspI AATATT 3 cut(s) 1693, 2344, 2358
SspMI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
SstI GAGCTC 2 cut(s) 223, 2307
StuI AGGCCT 1 cut(s) 3476
StyI CCWWGG 2 cut(s) 2098, 2199
TaiI ACGT 6 cut(s) 867, 973, 1183, 1701, 3559, 3587
TaqI TCGA 9 cut(s) 633, 663, 2282, 2361, 2484, 2543, 2604, 2918, 3811
TaqII GACCGA 1 cut(s) 2431
TatI WGTACW 7 cut(s) 77, 982, 1175, 1456, 2180, 2964, 3428
TauI GCSGC 4 cut(s) 152, 1642, 2504, 2522
TfiI GAWTC 9 cut(s) 101, 271, 2092, 2284, 2545, 2563, 3082, 3578, 3866
TscAI CASTG 8 cut(s) 358, 687, 1387, 1609, 2577, 2967, 3630, 3674
TseFI GTSAC 1 cut(s) 3625
Tsp45I GTSAC 1 cut(s) 3625
TspDTI ATGAA 8 cut(s) 194, 220, 522, 1179, 1544, 2527, 2630, 3575
TspGWI ACGGA 5 cut(s) 197, 1634, 2081, 2346, 3450
TspRI CASTG 8 cut(s) 358, 687, 1387, 1609, 2577, 2967, 3630, 3674
Tth111I GACNNNGTC 1 cut(s) 2492
Van91I CCANNNNNTGG 1 cut(s) 2205
VpaK11BI GGWCC 8 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877, 2443
VspI ATTAAT 1 cut(s) 740
XapI RAATTY 7 cut(s) 21, 378, 1166, 1616, 2211, 2616, 3547
XceI RCATGY 2 cut(s) 1344, 2659
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
XmiI GTMKAC 2 cut(s) 2238, 3165
XspI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2706, 3062
ZrmI AGTACT 1 cut(s) 2182
Zsp2I ATGCAT 1 cut(s) 3841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.