Rh5DG477100

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010845
Physical Location & Seq
Forward (+)
75406945 .. 75411679
4735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG477100.1

Sequence Viewer

Length: 2328 bp
ATGGAGTTTTTAGGTGTCGGAATTCGGACAAGCTATGGAGGTTTGTGGGCTAAATTGGCAATTTTACTGGTTCTGTGTACTATTGAACGAGCGGTGTGCGATTCCGACTATTTGATTGGGCTCGGGAGCTATGACATCACCGGACCGGCCGCTGATGTCAATATGATGGGGTATGCTAACACGGAGCAGATCGCTTCCGGAGTTCACTTCAGGCTGCGAGCTCGCTCGTTCATTGTGGCGGAGCCGAAGGGCAACCGAGTGGTGTTTGTGAATCTTGATGCTTGCATGGCCTCGCAGCTTGTGAAATTGAAAGTGATTGAGAGGTTGAAGGCAAGGTATGGGGATCTATACACTGAGAAGAATGTTGCTATTAGTGGAATTCACACCCATGCTGGCCCTGGGGGTTATCTACAGTATATTGTGTATATTGTAACATCTCTTGGGTTCGTGCGTCAGTCATTTGATGCCCTTGTTGATGGCATTGAGCAAAGTATTATTCAAGCCCATGATAATCTTGCGCCAGGATCAGTTTTTGTTAATAAGGGAGAAATCTTAGATGCTGGAGTAAACCGTAGTCCTAGTGCTTATCTCAATAATCCTGCAGCAGAGCGCAGTAAATACAAATATGATGTCGATAAAGAAATGACTCTTCTGAAGTTTGTCGATAATCAATGGGGTCCAGTGGGTAGCTTCAATTGGTTTGCAACTCATGGAACTTCTATGAGTCGGACAAACTCATTAATTAGTGGGGATAACAAGGGTGCTGCTGCAAGATTTATGGAAGACTGGTTTGAGGAGAATGGTGGTAGAAGTGCATATTCTGATGATATTGATGCTGCCGAGATACCGCGAAGAGTGTCAAACGTAGTTTCTGACCATCATGATAATCACCATGAGTTACTGGAGCTTGCTGCATCCTTTCAGTCTCCTCCTGGTAAGCCAGCAACCAGGTCCTTGAGTGTTGCAAGACGTGTCAGGGGTGTACTAAGGCAGGCTAACAAGCCTAGATTTGTTTCTGCCTTTTGTCAATCAAACTGTGGCGATGTGAGCCCAAATGTTCTAGGAGCTTTCTGCACAGATACTGGGCTACCTTGTGATTTCAATCACAGCACCTGTGGTGGGAAGAATGAGTTGTGCTATGGCCGAGGACCAGGTTACCCAGATGAATTTGAAAGTACACGTATTATTGCTGAGAGGCAATTGAGAAAAGCTGTGGATCTTTTCAACAAAGCATCTGAACAGTTGACTGGGAAGATTGAATATCGCCACACTTACCTCAACTTCTCCCAGCTTGAAGTAACCCTTCCCAAGAAGGGGGGAGGGTCTGAGGTGGTAAAGACATGTCCTGCTGCAATGGGATTTGGATTTGCTGCTGGAACCACTGATGGACCTGGAGCTTTTGATTTCAAGCAAGGGGATGATAAGGGAAATCCTTTCTGGAGATTAGTGCGCAATGTACTCAAAACACCAGGCCAGGAACAAATTGATTGTCAAAGTCCAAAGCCAATCTTGCTTGATACTGGTGAAATGAAGCAACCATATGACTGGGCGCCTTCAATACTTCCAATTCAGATCTTCCGGATAGGGCAGCTTGTCATTCTCAGTGTACCTGGAGAATTTACAACAATGGCTGGTAGGCGGCTCCGTGATGCTGTGAAAACCGTACTGACTAGTGGTGGTCATGGTGGAAATATTCATGTTGTGCTAGCAGGATTGACTAATACATATTCTCAGTATATCACGACCTTTGAAGAGTATGAGGTGCAGAGATATGAGGGTGCATCCACTCTCTATGGACCACACACGCTAAGTGCCTACATTCAGGAGTTCAAGAAGCTTGCTACAGCTCTCATCAGTAACCAACCTGTCGCATCAGGTCCACAACCCCCAGATCTCCTTGATAAGCAGATAAGCTTGCTTACTCCAGTTGTTATGGATGGAACCCCTGTTGGTGTCAGTTTTGGGGATTGCAGTTCGGATGTGCCTCAAAACTCCACCTTTAAGAGAGGTCATGACATGGTAACAGTTACCTTCTGGTCGGCTTGTCCTCGGAATGACCTATTGACGGAAGGTACATTTGCCCTAGTGGAGATTCTCCATGGAAAGGATACTTGGGTTCCAGCTTACGATGACGATGATTTCTGTCTGCGCTTTAAGTGGTCAAGACCTTCCAAACTCAGTACTAGAAGTCAGGCGACCATGGAATGGAGAATTCCTCAGTCCGCAACTCCCGGCGTATACAGAATAAGGCATTTTGGTGCCTCTAAGAGCCTGGTTGGATCGATTCGCCACTTTACGGGTTCATCTAGTGCTTTTGTGGTAGCATAA

Protein Analysis

775

Amino Acids

84.93

Weight (kDa)

6.81

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 37 - 608 2.8e-229 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 610 - 774 2.1e-54 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1451
AccB1I GGYRCC 2 cut(s) 1549, 2258
AccB7I CCANNNNNTGG 1 cut(s) 2205
AccBSI CCGCTC 1 cut(s) 92
AccI GTMKAC 1 cut(s) 2238
AccII CGCG 1 cut(s) 850
AccIII TCCGGA 2 cut(s) 197, 1578
AciI CCGC 6 cut(s) 92, 150, 239, 848, 1639, 2223
AclWI GGATC 4 cut(s) 351, 532, 1224, 2287
AcoI YGGCCR 2 cut(s) 147, 1141
AcsI RAATTY 5 cut(s) 21, 378, 1166, 1616, 2211
AcuI CTGAAG 2 cut(s) 193, 674
AcyI GRCGYC 1 cut(s) 1550
AfaI GTAC 8 cut(s) 79, 984, 1177, 1458, 1608, 1665, 2074, 2182
AfiI CCNNNNNNNGG 7 cut(s) 1119, 1313, 1314, 2065, 2104, 2205, 2296
AflIII ACRYGT 3 cut(s) 970, 1178, 1340
AhlI ACTAGT 1 cut(s) 1670
AjiI CACGTC 1 cut(s) 971
AjuI GAANNNNNNNTTGG 2 cut(s) 2095, 2127
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 4 cut(s) 351, 532, 1224, 2287
AlwNI CAGNNNCTG 2 cut(s) 1082, 1113
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 2 cut(s) 197, 1578
AoxI GGCC 5 cut(s) 147, 288, 394, 1141, 1471
ApoI RAATTY 5 cut(s) 21, 378, 1166, 1616, 2211
ArsI GACNNNNNNTTYG 4 cut(s) 615, 647, 1474, 1506
AseI ATTAAT 1 cut(s) 740
AspLEI GCGC 5 cut(s) 520, 612, 1452, 1552, 2151
AspS9I GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
AsuC2I CCSGG 1 cut(s) 2232
AsuHPI GGTGA 3 cut(s) 130, 881, 1535
AsuNHI GCTAGC 1 cut(s) 1705
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
BaeI ACNNNNGTAYC 2 cut(s) 2100, 2133
BanI GGYRCC 2 cut(s) 1549, 2258
BanII GRGCYC 3 cut(s) 123, 223, 1052
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 1 cut(s) 223
BccI CCATC 5 cut(s) 160, 470, 885, 1379, 1931
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciVI GTATCC 1 cut(s) 2101
BcnI CCSGG 1 cut(s) 2232
BcoDI GTCTC 1 cut(s) 930
BcuI ACTAGT 1 cut(s) 1670
BfaI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2084, 2184, 2307
BfmI CTRYAG 3 cut(s) 410, 600, 1842
BfoI RGCGCY 1 cut(s) 1553
BfuI GTATCC 1 cut(s) 2101
BglII AGATCT 2 cut(s) 1572, 1891
BmcAI AGTACT 1 cut(s) 2182
Bme18I GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
BmeT110I CYCGRG 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 971
BmgT120I GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
BmiI GGNNCC 8 cut(s) 243, 678, 1378, 1551, 1643, 1942, 2118, 2260
BmrI ACTGGG 3 cut(s) 1092, 1257, 1555
BmsI GCATC 9 cut(s) 268, 454, 547, 823, 923, 1241, 1639, 1790, 1880
BmtI GCTAGC 1 cut(s) 1709
BmuI ACTGGG 3 cut(s) 1092, 1257, 1555
BpiI GAAGAC 1 cut(s) 789
BplI GAGNNNNNCTC 2 cut(s) 2200, 2232
BpmI CTGGAG 6 cut(s) 582, 923, 1413, 1459, 1632, 1908
BpuEI CTTGAG 1 cut(s) 976
BpuMI CCSGG 1 cut(s) 2232
Bsa29I ATCGAT 1 cut(s) 2282
BsaAI YACGTR 1 cut(s) 1181
BsaBI GATNNNNATC 1 cut(s) 1101
BsaHI GRCGYC 1 cut(s) 1550
BsaJI CCNNGG 6 cut(s) 397, 398, 1144, 2048, 2098, 2199
BsaWI WCCGGW 3 cut(s) 140, 197, 1578
Bsc4I CCNNNNNNNGG 7 cut(s) 1119, 1313, 1314, 2065, 2104, 2205, 2296
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 9 cut(s) 72, 680, 791, 906, 1087, 1252, 1525, 1550, 1925
Bse3DI GCAATG 2 cut(s) 1359, 1459
Bse8I GATNNNNATC 1 cut(s) 1101
BseAI TCCGGA 2 cut(s) 197, 1578
BseCI ATCGAT 1 cut(s) 2282
BseDI CCNNGG 6 cut(s) 397, 398, 1144, 2048, 2098, 2199
BseGI GGATG 5 cut(s) 914, 1423, 1781, 1942, 1984
BseJI GATNNNNATC 1 cut(s) 1101
BseLI CCNNNNNNNGG 7 cut(s) 1119, 1313, 1314, 2065, 2104, 2205, 2296
BseMI GCAATG 2 cut(s) 1359, 1459
BseMII CTCAG 7 cut(s) 345, 1182, 1317, 1615, 1745, 2191, 2231
BseNI ACTGG 9 cut(s) 72, 680, 791, 906, 1087, 1252, 1525, 1550, 1925
BseRI GAGGAG 2 cut(s) 809, 918
BseX3I CGGCCG 1 cut(s) 147
BseYI CCCAGC 1 cut(s) 1287
BsgI GTGCAG 2 cut(s) 1057, 1784
Bsh1236I CGCG 1 cut(s) 850
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 5 cut(s) 149, 290, 396, 1143, 1473
BshNI GGYRCC 2 cut(s) 1549, 2258
BshVI ATCGAT 1 cut(s) 2282
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 5 cut(s) 141, 146, 198, 1579, 2232
BslI CCNNNNNNNGG 7 cut(s) 1119, 1313, 1314, 2065, 2104, 2205, 2296
BsmAI GTCTC 1 cut(s) 930
BsnI GGCC 5 cut(s) 149, 290, 396, 1143, 1473
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 123, 223, 1052
Bsp13I TCCGGA 2 cut(s) 197, 1578
Bsp143I GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
Bsp19I CCATGG 2 cut(s) 2098, 2199
BspACI CCGC 6 cut(s) 92, 150, 239, 848, 1639, 2223
BspANI GGCC 5 cut(s) 149, 290, 396, 1143, 1473
BspCNI CTCAG 7 cut(s) 346, 1183, 1318, 1614, 1744, 2190, 2230
BspDI ATCGAT 1 cut(s) 2282
BspEI TCCGGA 2 cut(s) 197, 1578
BspFNI CGCG 1 cut(s) 850
BspHI TCATGA 2 cut(s) 880, 2011
BspLI GGNNCC 8 cut(s) 243, 678, 1378, 1551, 1643, 1942, 2118, 2260
BspMAI CTGCAG 1 cut(s) 604
BspOI GCTAGC 1 cut(s) 1709
BspPI GGATC 4 cut(s) 351, 532, 1224, 2287
BspT107I GGYRCC 2 cut(s) 1549, 2258
BsrBI CCGCTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 1359, 1459
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 9 cut(s) 72, 680, 791, 906, 1087, 1252, 1525, 1550, 1925
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 6 cut(s) 397, 398, 1144, 2048, 2098, 2199
BssMI GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
BssNAI GTATAC 1 cut(s) 2239
BssNI GRCGYC 1 cut(s) 1550
BssT1I CCWWGG 2 cut(s) 2098, 2199
Bst1107I GTATAC 1 cut(s) 2239
Bst4CI ACNGT 6 cut(s) 414, 572, 1037, 1242, 1663, 2026
Bst6I CTCTTC 3 cut(s) 654, 847, 1746
BstACI GRCGYC 1 cut(s) 1550
BstBAI YACGTR 1 cut(s) 1181
BstDSI CCRYGG 2 cut(s) 2098, 2199
BstEII GGTNACC 1 cut(s) 1154
BstF5I GGATG 5 cut(s) 914, 1423, 1781, 1942, 1984
BstFNI CGCG 1 cut(s) 850
BstH2I RGCGCY 1 cut(s) 1553
BstHHI GCGC 5 cut(s) 520, 612, 1452, 1552, 2151
BstKTI GATC 7 cut(s) 192, 346, 527, 1219, 1575, 1894, 2282
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 4 cut(s) 56, 287, 1047, 1510
BstNSI RCATGY 1 cut(s) 1344
BstPI GGTNACC 1 cut(s) 1154
BstSFI CTRYAG 3 cut(s) 410, 600, 1842
BstUI CGCG 1 cut(s) 850
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 4 cut(s) 343, 1216, 1572, 1891
BstXI CCANNNNNNTGG 1 cut(s) 1545
BstYI RGATCY 4 cut(s) 343, 1216, 1572, 1891
BstZ17I GTATAC 1 cut(s) 2239
BstZI CGGCCG 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 2282
BsuI GTATCC 1 cut(s) 2101
BsuRI GGCC 5 cut(s) 149, 290, 396, 1143, 1473
BsuTUI ATCGAT 1 cut(s) 2282
BtgI CCRYGG 2 cut(s) 2098, 2199
BtgZI GCGATG 1 cut(s) 1056
BtrI CACGTC 1 cut(s) 971
BtsCI GGATG 5 cut(s) 914, 1423, 1781, 1942, 1984
BtsIMutI CAGTG 4 cut(s) 351, 687, 1380, 1609
CaiI CAGNNNCTG 2 cut(s) 1082, 1113
CciI TCATGA 2 cut(s) 880, 2011
CfoI GCGC 5 cut(s) 520, 612, 1452, 1552, 2151
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
ClaI ATCGAT 1 cut(s) 2282
CpoI CGGWCCG 1 cut(s) 143
CseI GACGC 1 cut(s) 440
CsiI ACCWGGT 2 cut(s) 947, 1150
Csp6I GTAC 8 cut(s) 78, 983, 1176, 1457, 1607, 1664, 2073, 2181
CspI CGGWCCG 1 cut(s) 143
CviQI GTAC 8 cut(s) 78, 983, 1176, 1457, 1607, 1664, 2073, 2181
DinI GGCGCC 1 cut(s) 1551
DpnI GATC 7 cut(s) 191, 345, 526, 1218, 1574, 1893, 2281
DpnII GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
EaeI YGGCCR 2 cut(s) 147, 1141
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 3 cut(s) 654, 847, 1746
EarI CTCTTC 3 cut(s) 654, 847, 1746
EciI GGCGGA 1 cut(s) 254
Ecl136II GAGCTC 1 cut(s) 221
EclXI CGGCCG 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 2098, 2199
Eco24I GRGCYC 3 cut(s) 123, 223, 1052
Eco47I GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
Eco52I CGGCCG 1 cut(s) 147
Eco53kI GAGCTC 1 cut(s) 221
Eco57I CTGAAG 2 cut(s) 193, 674
Eco88I CYCGRG 1 cut(s) 122
Eco91I GGTNACC 1 cut(s) 1154
EcoICRI GAGCTC 1 cut(s) 221
EcoO109I RGGNCCY 1 cut(s) 951
EcoO65I GGTNACC 1 cut(s) 1154
EcoRI GAATTC 3 cut(s) 21, 378, 2211
EcoT14I CCWWGG 2 cut(s) 2098, 2199
EcoT38I GRGCYC 3 cut(s) 123, 223, 1052
EgeI GGCGCC 1 cut(s) 1551
EheI GGCGCC 1 cut(s) 1551
ErhI CCWWGG 2 cut(s) 2098, 2199
FalI AAGNNNNNCTT 4 cut(s) 1287, 1319, 1493, 1525
FauNDI CATATG 1 cut(s) 1540
FblI GTMKAC 1 cut(s) 2238
FokI GGATG 5 cut(s) 901, 1430, 1768, 1949, 1991
FriOI GRGCYC 3 cut(s) 123, 223, 1052
FspBI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2084, 2184, 2307
FspI TGCGCA 1 cut(s) 1451
GlaI GCGC 5 cut(s) 519, 611, 1451, 1551, 2150
GsaI CCCAGC 1 cut(s) 1291
GsuI CTGGAG 6 cut(s) 582, 923, 1413, 1459, 1632, 1908
HaeII RGCGCY 1 cut(s) 1553
HaeIII GGCC 5 cut(s) 149, 290, 396, 1143, 1473
HapII CCGG 5 cut(s) 141, 146, 198, 1579, 2232
HgaI GACGC 1 cut(s) 440
HhaI GCGC 5 cut(s) 520, 612, 1452, 1552, 2151
Hin1I GRCGYC 1 cut(s) 1550
Hin6I GCGC 5 cut(s) 518, 610, 1450, 1550, 2149
HinP1I GCGC 5 cut(s) 518, 610, 1450, 1550, 2149
HincII GTYRAC 1 cut(s) 1245
HindII GTYRAC 1 cut(s) 1245
HindIII AAGCTT 2 cut(s) 1835, 1912
HinfI GANTC 6 cut(s) 101, 271, 646, 724, 2092, 2284
HpaII CCGG 5 cut(s) 141, 146, 198, 1579, 2232
HphI GGTGA 3 cut(s) 130, 881, 1535
Hpy166II GTNNAC 9 cut(s) 78, 205, 568, 983, 1178, 1245, 1607, 1880, 2239
Hpy8I GTNNAC 9 cut(s) 78, 205, 568, 983, 1178, 1245, 1607, 1880, 2239
HpyAV CCTTC 8 cut(s) 241, 322, 1306, 1313, 1563, 2041, 2063, 2178
HpyCH4III ACNGT 6 cut(s) 414, 572, 1037, 1242, 1663, 2026
HpyCH4IV ACGT 3 cut(s) 864, 970, 1180
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 287, 1047, 1510
HpySE526I ACGT 3 cut(s) 864, 970, 1180
Hsp92I GRCGYC 1 cut(s) 1550
HspAI GCGC 5 cut(s) 518, 610, 1450, 1550, 2149
KasI GGCGCC 1 cut(s) 1549
Kpn2I TCCGGA 2 cut(s) 197, 1578
Kzo9I GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
LmnI GCTCC 7 cut(s) 126, 184, 241, 904, 1064, 1394, 1647
LweI GCATC 9 cut(s) 268, 454, 547, 823, 923, 1241, 1639, 1790, 1880
MabI ACCWGGT 2 cut(s) 947, 1150
MaeI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2084, 2184, 2307
MaeII ACGT 3 cut(s) 864, 970, 1180
MaeIII GTNAC 7 cut(s) 430, 897, 1154, 1297, 1856, 2020, 2026
MalI GATC 7 cut(s) 191, 345, 526, 1218, 1574, 1893, 2281
MbiI CCGCTC 1 cut(s) 92
MboI GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
MboII GAAGA 8 cut(s) 370, 641, 794, 864, 1135, 1264, 1567, 1763
MfeI CAATTG 2 cut(s) 694, 1199
MflI RGATCY 4 cut(s) 343, 1216, 1572, 1891
MhlI GDGCHC 3 cut(s) 123, 223, 1052
Mly113I GGCGCC 1 cut(s) 1550
MlyI GAGTC 2 cut(s) 640, 733
MmeI TCCRAC 3 cut(s) 129, 707, 2257
MroI TCCGGA 2 cut(s) 197, 1578
MseI TTAA 4 cut(s) 537, 740, 2001, 2154
MslI CAYNNNNRTG 2 cut(s) 387, 2256
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 5 cut(s) 141, 146, 198, 1579, 2232
MunI CAATTG 2 cut(s) 694, 1199
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 4 cut(s) 56, 287, 1047, 1510
NarI GGCGCC 1 cut(s) 1550
NciI CCSGG 1 cut(s) 2232
NcoI CCATGG 2 cut(s) 2098, 2199
NdeI CATATG 1 cut(s) 1540
NdeII GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
NheI GCTAGC 1 cut(s) 1705
NlaIV GGNNCC 8 cut(s) 243, 678, 1378, 1551, 1643, 1942, 2118, 2260
NmeAIII GCCGAG 2 cut(s) 865, 1169
NsbI TGCGCA 1 cut(s) 1451
NspI RCATGY 1 cut(s) 1344
PagI TCATGA 2 cut(s) 880, 2011
PasI CCCWGGG 1 cut(s) 398
PciI ACATGT 1 cut(s) 1340
PfeI GAWTC 4 cut(s) 101, 271, 2092, 2284
PflMI CCANNNNNTGG 1 cut(s) 2205
PleI GAGTC 2 cut(s) 640, 732
PluTI GGCGCC 1 cut(s) 1553
PpsI GAGTC 2 cut(s) 640, 732
Ppu21I YACGTR 1 cut(s) 1181
PpuMI RGGWCCY 1 cut(s) 951
PscI ACATGT 1 cut(s) 1340
PshBI ATTAAT 1 cut(s) 740
Psp124BI GAGCTC 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 951
PspEI GGTNACC 1 cut(s) 1154
PspFI CCCAGC 1 cut(s) 1287
PspN4I GGNNCC 8 cut(s) 243, 678, 1378, 1551, 1643, 1942, 2118, 2260
PspPI GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
PspPPI RGGWCCY 1 cut(s) 951
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 2 cut(s) 1082, 1113
PsuI RGATCY 4 cut(s) 343, 1216, 1572, 1891
RsaI GTAC 8 cut(s) 79, 984, 1177, 1458, 1608, 1665, 2074, 2182
RsaNI GTAC 8 cut(s) 78, 983, 1176, 1457, 1607, 1664, 2073, 2181
RseI CAYNNNNRTG 2 cut(s) 387, 2256
Rsr2I CGGWCCG 1 cut(s) 143
RsrII CGGWCCG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 223
SaqAI TTAA 4 cut(s) 537, 740, 2001, 2154
Sau3AI GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
Sau96I GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
ScaI AGTACT 1 cut(s) 2182
SchI GAGTC 2 cut(s) 640, 733
SduI GDGCHC 3 cut(s) 123, 223, 1052
SexAI ACCWGGT 2 cut(s) 947, 1150
SfaNI GCATC 9 cut(s) 268, 454, 547, 823, 923, 1241, 1639, 1790, 1880
SfcI CTRYAG 3 cut(s) 410, 600, 1842
SfoI GGCGCC 1 cut(s) 1551
SinI GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
SmiMI CAYNNNNRTG 2 cut(s) 387, 2256
SmlI CTYRAG 1 cut(s) 955
SmoI CTYRAG 1 cut(s) 955
SpeI ACTAGT 1 cut(s) 1670
SsiI CCGC 6 cut(s) 92, 150, 239, 848, 1639, 2223
SspDI GGCGCC 1 cut(s) 1549
SspI AATATT 1 cut(s) 1693
SspMI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2084, 2184, 2307
SstI GAGCTC 1 cut(s) 223
StyI CCWWGG 2 cut(s) 2098, 2199
TaaI ACNGT 6 cut(s) 414, 572, 1037, 1242, 1663, 2026
TaiI ACGT 3 cut(s) 867, 973, 1183
TaqI TCGA 3 cut(s) 633, 663, 2282
TatI WGTACW 5 cut(s) 77, 982, 1175, 1456, 2180
TauI GCSGC 2 cut(s) 152, 1642
TfiI GAWTC 4 cut(s) 101, 271, 2092, 2284
Tru1I TTAA 4 cut(s) 537, 740, 2001, 2154
Tru9I TTAA 4 cut(s) 537, 740, 2001, 2154
TscAI CASTG 4 cut(s) 358, 687, 1387, 1609
TspDTI ATGAA 5 cut(s) 220, 1179, 1544, 1685, 2292
TspGWI ACGGA 3 cut(s) 197, 1634, 2081
TspRI CASTG 4 cut(s) 358, 687, 1387, 1609
Van91I CCANNNNNTGG 1 cut(s) 2205
VpaK11BI GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
VspI ATTAAT 1 cut(s) 740
XapI RAATTY 5 cut(s) 21, 378, 1166, 1616, 2211
XceI RCATGY 1 cut(s) 1344
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 2238
XspI CTAG 8 cut(s) 579, 1005, 1061, 1671, 1706, 2084, 2184, 2307
ZrmI AGTACT 1 cut(s) 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.