RchiOBHm_Chr5g0067951

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010845
Physical Location & Seq
Forward (+)
74039589 .. 74044931
5343 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34360

Sequence Viewer

Length: 2328 bp
ATGGAGTTTTTAGGTGTCGGAATTCGGAAAAGCTATGGAGGTTTGTGGGCTAAATTGGCAATTTTACTGGTTCTGAGTACTATTGAACGAGCGGTGTGCGATTCCGACTATTTGATTGGGCTCGGGAGCTATGACATCACCGGACCGGCCGCTGATGTCAATATGATGGGGTATGCTAACACGGAGCAGATCGCTTCCGGAGTTCACTTCAGGCTGCGAGCTCGCTCGTTCATTGTGGCGGAGCCGAAGGGCAACCGAGTGGTGTTTGTGAATCTTGATGCTTGCATGGCCTCGCAGCTTGTGAAATTGAAAGTTATTGAGAGGTTGAAGGCAAGGTATGGGGATCTATACACTGAGAAGAATGTAGCTATTAGTGGAATTCACACCCATGCTGGCCCTGGGGGTTATCTACAGTATATTGTGTATATTGTAACATCTCTTGGGTTCGTGCGTCAGTCATTTGATGCCCTTGTTGATGGCATTCAGCAAAGTATTATTCAAGCCCATGATAATCTTGCGCCAGGATCAGTTTTTGTTAATAAGGGAGAAATCTTAGATGCTGGAGTAAACCGTAGTCCTAGTGCTTATCTCAATAATCCTGCAGCAGAGCGCAGTAAATACAAATATGATGTCGATAAAGAAATGACTCTTCTGAAGTTTGTCGATAATCAATGGGGTCCAGTGGGTAGCTTCAATTGGTTTGCAACTCATGGAACTTCTATGAGTCGGACAAACTCATTAATTAGTGGGGATAACAAGGGTGCTGCTGCACGATTTATGGAAGACTGGTTTGAGGAGAATGGTGGTAGAAGTGCATATTCTGATGATATTGATGCTGCCGATATGCCGCGAAGAGTGTCAAACATAGTTTCTGACCATCATGATAATCACCATGAGTTACTGGAGCTTGCTGCATCCTTTCAGTCTCCTCCTGGTAAGCCAGCAACCAGGTCCTTGAGTGTTGCAAGACGTGTCAGGGGTGTACTAAGGCAGGCTAACAAGCCTAGATTTGTTTCTGCCTTTTGTCAATCAAACTGTGGTGATGTGAGCCCAAATGTTCTAGGAGCTTTCTGCACAGATACTGGGCTACCTTGTGATTTCAATCACAGCACCTGTGGTGGGAAGAATGAGTTGTGCTATGGCCGAGGACCGGGTTACCCAGATGAATTTGAAAGTACACGTATTATTGCTGAGAGGCAACTGAGAAAAGCTGTGGATCTTTTCAACAAAGCATCTGAACAGTTGACCGGGAAGATTGAATATCGCCACACTTACCTCAACTTCTCCCAGCTTGAAGTAACCCTTCCCAAGAAGGGGGGAGGTTCTGAGGTGGTAAAGACATGTCCTGCTGCAATGGGATTCGGATTTGCTGCTGGAACCACTGATGGACCTGGAGCTTTTGATTTCAAGCAAGGGGATGATAAGGGAAATCCTTTCTGGAGATTAGTGCGCGATGTACTCAAAACACCAGGCCAGGAACAAATTGATTGTCAGCGTCCAAAGCCAATCTTGCTTGATACTGGTGAAATGAAGCAACCATATGACTGGGCGCCTTCAATACTTCCAATTCAGATCTTCCGGATAGGGCAGCTTGTCATTCTCAGTGTACCTGGAGAATTTACAACAATGGCTGGTAGGCGGCTCCGTGATGCTGTGAAAACCATACTGACTAGTGCTGGTCATGGTGGAAATATTCATGTTGTGCTAGCAGGATTGACTAATACATATTCTCAGTATATCACGACCTTTGAAGAGTATGAGGTGCAGAGATATGAGGGTGCATCCACTCTCTATGGACCACACACGCTAAGTGCCTACATTCAGGAGTTCAAGAAGCTTGCTACAGCTCTCATCAGTAACCAACCCGTCGCATCAGGTCCACAACCCCCAGATCTCCTTGATAAGCAGATAAGCTTGCTTACTCCAGTTGTTATGGATGCAACCCCTGTTGGTGTCAGTTTTGGGGATTGCAGTTCGGATGTGCCTCAAAACTCCACCTTTAAGAGAGGTCATGACATGGTAACAGTTACCTTCTGGTCGGCTTGTCCTCGGAATGACCTATTGACGGAAGGTACATTTGCCCTTGTGGAGATTCTCCATGGAAAGGATACTTGGGTTCCAGCTTACGATGACGATGATTTCTGTCTGCGCTTTAAGTGGTCAAGACCTTCCAAACTCAGTACTAGAAGTCAGGCCACCATGGAATGGAGAATTCCTCAGTCCGCAACTCCCGGCGTATACAGAATAAGGCATTTTGGTGCCTCCAAGAGCCTGGTTGGATCGATTCGCCACTTTACGGGTTCATCTAGTGCTTTTGTAGTAGCATAA

Protein Analysis

775

Amino Acids

85.07

Weight (kDa)

7.26

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 37 - 608 1.7e-229 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 610 - 774 1.8e-54 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1549, 2258
AccB7I CCANNNNNTGG 1 cut(s) 2205
AccBSI CCGCTC 1 cut(s) 92
AccI GTMKAC 1 cut(s) 2238
AccII CGCG 2 cut(s) 850, 1452
AccIII TCCGGA 2 cut(s) 197, 1578
AciI CCGC 6 cut(s) 92, 150, 239, 848, 1639, 2223
AclWI GGATC 4 cut(s) 351, 532, 1224, 2287
AcoI YGGCCR 2 cut(s) 147, 1141
AcsI RAATTY 5 cut(s) 21, 378, 1166, 1616, 2211
AcuI CTGAAG 2 cut(s) 193, 674
AcyI GRCGYC 1 cut(s) 1550
AfaI GTAC 7 cut(s) 79, 984, 1177, 1458, 1608, 2074, 2182
AfiI CCNNNNNNNGG 8 cut(s) 1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296
AflIII ACRYGT 3 cut(s) 970, 1178, 1340
AhlI ACTAGT 1 cut(s) 1670
AjiI CACGTC 1 cut(s) 971
AjnI CCWGG 9 cut(s) 397, 520, 931, 947, 1390, 1468, 1473, 1609, 2271
AjuI GAANNNNNNNTTGG 2 cut(s) 2095, 2127
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 4 cut(s) 351, 532, 1224, 2287
AlwNI CAGNNNCTG 2 cut(s) 1082, 1113
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 2 cut(s) 197, 1578
AoxI GGCC 6 cut(s) 147, 288, 394, 1141, 1471, 2193
ApoI RAATTY 5 cut(s) 21, 378, 1166, 1616, 2211
ArsI GACNNNNNNTTYG 4 cut(s) 615, 647, 1474, 1506
AseI ATTAAT 1 cut(s) 740
AspLEI GCGC 5 cut(s) 520, 612, 1452, 1552, 2151
AspS9I GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
AsuC2I CCSGG 3 cut(s) 1152, 1249, 2232
AsuHPI GGTGA 4 cut(s) 130, 881, 1052, 1535
AsuNHI GCTAGC 1 cut(s) 1705
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
BaeI ACNNNNGTAYC 2 cut(s) 2100, 2133
BanI GGYRCC 2 cut(s) 1549, 2258
BanII GRGCYC 3 cut(s) 123, 223, 1052
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 1 cut(s) 223
BccI CCATC 4 cut(s) 160, 470, 885, 1379
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 9 cut(s) 399, 522, 933, 949, 1392, 1470, 1475, 1611, 2273
BciVI GTATCC 1 cut(s) 2101
BcnI CCSGG 3 cut(s) 1152, 1249, 2232
BcoDI GTCTC 1 cut(s) 930
BcuI ACTAGT 1 cut(s) 1670
BfaI CTAG 7 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2307
BfmI CTRYAG 3 cut(s) 410, 600, 1842
BfoI RGCGCY 1 cut(s) 1553
BfuI GTATCC 1 cut(s) 2101
BglII AGATCT 2 cut(s) 1572, 1891
BmcAI AGTACT 2 cut(s) 79, 2182
Bme18I GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
BmeT110I CYCGRG 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 971
BmgT120I GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
BmiI GGNNCC 7 cut(s) 243, 678, 1378, 1551, 1643, 2118, 2260
BmrI ACTGGG 2 cut(s) 1092, 1555
BmtI GCTAGC 1 cut(s) 1709
BmuI ACTGGG 2 cut(s) 1092, 1555
BpiI GAAGAC 1 cut(s) 789
BplI GAGNNNNNCTC 2 cut(s) 2200, 2232
BpmI CTGGAG 6 cut(s) 582, 923, 1413, 1459, 1632, 1908
BpuEI CTTGAG 1 cut(s) 976
BpuMI CCSGG 3 cut(s) 1152, 1249, 2232
Bsa29I ATCGAT 1 cut(s) 2282
BsaAI YACGTR 1 cut(s) 1181
BsaBI GATNNNNATC 1 cut(s) 1101
BsaHI GRCGYC 1 cut(s) 1550
BsaJI CCNNGG 6 cut(s) 397, 398, 1144, 2048, 2098, 2199
BsaWI WCCGGW 3 cut(s) 140, 197, 1578
Bsc4I CCNNNNNNNGG 8 cut(s) 1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 8 cut(s) 72, 680, 791, 906, 1087, 1525, 1550, 1925
Bse3DI GCAATG 1 cut(s) 1359
Bse8I GATNNNNATC 1 cut(s) 1101
BseAI TCCGGA 2 cut(s) 197, 1578
BseBI CCWGG 9 cut(s) 399, 522, 933, 949, 1392, 1470, 1475, 1611, 2273
BseCI ATCGAT 1 cut(s) 2282
BseDI CCNNGG 6 cut(s) 397, 398, 1144, 2048, 2098, 2199
BseGI GGATG 5 cut(s) 914, 1423, 1781, 1942, 1984
BseJI GATNNNNATC 1 cut(s) 1101
BseLI CCNNNNNNNGG 8 cut(s) 1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296
BseMI GCAATG 1 cut(s) 1359
BseMII CTCAG 9 cut(s) 65, 345, 1182, 1193, 1317, 1615, 1745, 2191, 2231
BseNI ACTGG 8 cut(s) 72, 680, 791, 906, 1087, 1525, 1550, 1925
BseRI GAGGAG 2 cut(s) 809, 918
BseX3I CGGCCG 1 cut(s) 147
BseYI CCCAGC 1 cut(s) 1287
BsgI GTGCAG 3 cut(s) 753, 1057, 1784
Bsh1236I CGCG 2 cut(s) 850, 1452
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 6 cut(s) 149, 290, 396, 1143, 1473, 2195
BshNI GGYRCC 2 cut(s) 1549, 2258
BshVI ATCGAT 1 cut(s) 2282
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 7 cut(s) 141, 146, 198, 1151, 1248, 1579, 2232
BslI CCNNNNNNNGG 8 cut(s) 1119, 1150, 1313, 1314, 2065, 2104, 2205, 2296
BsmAI GTCTC 1 cut(s) 930
BsmI GAATGC 1 cut(s) 480
BsnI GGCC 6 cut(s) 149, 290, 396, 1143, 1473, 2195
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 123, 223, 1052
Bsp13I TCCGGA 2 cut(s) 197, 1578
Bsp143I GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
Bsp19I CCATGG 2 cut(s) 2098, 2199
BspACI CCGC 6 cut(s) 92, 150, 239, 848, 1639, 2223
BspANI GGCC 6 cut(s) 149, 290, 396, 1143, 1473, 2195
BspCNI CTCAG 9 cut(s) 66, 346, 1183, 1194, 1318, 1614, 1744, 2190, 2230
BspDI ATCGAT 1 cut(s) 2282
BspEI TCCGGA 2 cut(s) 197, 1578
BspFNI CGCG 2 cut(s) 850, 1452
BspHI TCATGA 2 cut(s) 880, 2011
BspLI GGNNCC 7 cut(s) 243, 678, 1378, 1551, 1643, 2118, 2260
BspMAI CTGCAG 1 cut(s) 604
BspOI GCTAGC 1 cut(s) 1709
BspPI GGATC 4 cut(s) 351, 532, 1224, 2287
BspT107I GGYRCC 2 cut(s) 1549, 2258
BsrBI CCGCTC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 1359
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 8 cut(s) 72, 680, 791, 906, 1087, 1525, 1550, 1925
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 6 cut(s) 397, 398, 1144, 2048, 2098, 2199
BssMI GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
BssNAI GTATAC 1 cut(s) 2239
BssNI GRCGYC 1 cut(s) 1550
BssT1I CCWWGG 2 cut(s) 2098, 2199
Bst1107I GTATAC 1 cut(s) 2239
Bst2UI CCWGG 9 cut(s) 399, 522, 933, 949, 1392, 1470, 1475, 1611, 2273
Bst4CI ACNGT 5 cut(s) 414, 572, 1037, 1242, 2026
Bst6I CTCTTC 3 cut(s) 654, 847, 1746
BstACI GRCGYC 1 cut(s) 1550
BstBAI YACGTR 1 cut(s) 1181
BstDSI CCRYGG 2 cut(s) 2098, 2199
BstEII GGTNACC 1 cut(s) 1154
BstF5I GGATG 5 cut(s) 914, 1423, 1781, 1942, 1984
BstFNI CGCG 2 cut(s) 850, 1452
BstH2I RGCGCY 1 cut(s) 1553
BstHHI GCGC 5 cut(s) 520, 612, 1452, 1552, 2151
BstKTI GATC 7 cut(s) 192, 346, 527, 1219, 1575, 1894, 2282
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 4 cut(s) 56, 287, 1501, 1510
BstNI CCWGG 9 cut(s) 399, 522, 933, 949, 1392, 1470, 1475, 1611, 2273
BstNSI RCATGY 1 cut(s) 1344
BstPI GGTNACC 1 cut(s) 1154
BstSFI CTRYAG 3 cut(s) 410, 600, 1842
BstUI CGCG 2 cut(s) 850, 1452
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 4 cut(s) 343, 1216, 1572, 1891
BstXI CCANNNNNNTGG 2 cut(s) 1545, 2272
BstYI RGATCY 4 cut(s) 343, 1216, 1572, 1891
BstZ17I GTATAC 1 cut(s) 2239
BstZI CGGCCG 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 2282
BsuI GTATCC 1 cut(s) 2101
BsuRI GGCC 6 cut(s) 149, 290, 396, 1143, 1473, 2195
BsuTUI ATCGAT 1 cut(s) 2282
BtgI CCRYGG 2 cut(s) 2098, 2199
BtgZI GCGATG 1 cut(s) 1467
BtrI CACGTC 1 cut(s) 971
BtsCI GGATG 5 cut(s) 914, 1423, 1781, 1942, 1984
BtsIMutI CAGTG 4 cut(s) 351, 687, 1380, 1609
CaiI CAGNNNCTG 2 cut(s) 1082, 1113
CciI TCATGA 2 cut(s) 880, 2011
CfoI GCGC 5 cut(s) 520, 612, 1452, 1552, 2151
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
ClaI ATCGAT 1 cut(s) 2282
CpoI CGGWCCG 1 cut(s) 143
CseI GACGC 2 cut(s) 440, 1484
CsiI ACCWGGT 1 cut(s) 947
Csp6I GTAC 7 cut(s) 78, 983, 1176, 1457, 1607, 2073, 2181
CspI CGGWCCG 1 cut(s) 143
CviQI GTAC 7 cut(s) 78, 983, 1176, 1457, 1607, 2073, 2181
DinI GGCGCC 1 cut(s) 1551
DpnI GATC 7 cut(s) 191, 345, 526, 1218, 1574, 1893, 2281
DpnII GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
EaeI YGGCCR 2 cut(s) 147, 1141
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 3 cut(s) 654, 847, 1746
EarI CTCTTC 3 cut(s) 654, 847, 1746
EciI GGCGGA 1 cut(s) 254
Ecl136II GAGCTC 1 cut(s) 221
EclXI CGGCCG 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 2098, 2199
Eco24I GRGCYC 3 cut(s) 123, 223, 1052
Eco47I GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
Eco52I CGGCCG 1 cut(s) 147
Eco53kI GAGCTC 1 cut(s) 221
Eco57I CTGAAG 2 cut(s) 193, 674
Eco88I CYCGRG 1 cut(s) 122
Eco91I GGTNACC 1 cut(s) 1154
EcoICRI GAGCTC 1 cut(s) 221
EcoO109I RGGNCCY 1 cut(s) 951
EcoO65I GGTNACC 1 cut(s) 1154
EcoRI GAATTC 3 cut(s) 21, 378, 2211
EcoRII CCWGG 9 cut(s) 397, 520, 931, 947, 1390, 1468, 1473, 1609, 2271
EcoT14I CCWWGG 2 cut(s) 2098, 2199
EcoT38I GRGCYC 3 cut(s) 123, 223, 1052
EgeI GGCGCC 1 cut(s) 1551
EheI GGCGCC 1 cut(s) 1551
ErhI CCWWGG 2 cut(s) 2098, 2199
FalI AAGNNNNNCTT 4 cut(s) 1287, 1319, 1493, 1525
FauNDI CATATG 1 cut(s) 1540
FblI GTMKAC 1 cut(s) 2238
FokI GGATG 5 cut(s) 901, 1430, 1768, 1949, 1991
FriOI GRGCYC 3 cut(s) 123, 223, 1052
FspBI CTAG 7 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2307
GlaI GCGC 5 cut(s) 519, 611, 1451, 1551, 2150
GsaI CCCAGC 1 cut(s) 1291
GsuI CTGGAG 6 cut(s) 582, 923, 1413, 1459, 1632, 1908
HaeII RGCGCY 1 cut(s) 1553
HaeIII GGCC 6 cut(s) 149, 290, 396, 1143, 1473, 2195
HapII CCGG 7 cut(s) 141, 146, 198, 1151, 1248, 1579, 2232
HgaI GACGC 2 cut(s) 440, 1484
HhaI GCGC 5 cut(s) 520, 612, 1452, 1552, 2151
Hin1I GRCGYC 1 cut(s) 1550
Hin6I GCGC 5 cut(s) 518, 610, 1450, 1550, 2149
HinP1I GCGC 5 cut(s) 518, 610, 1450, 1550, 2149
HincII GTYRAC 1 cut(s) 1245
HindII GTYRAC 1 cut(s) 1245
HindIII AAGCTT 2 cut(s) 1835, 1912
HinfI GANTC 7 cut(s) 101, 271, 646, 724, 1359, 2092, 2284
HpaII CCGG 7 cut(s) 141, 146, 198, 1151, 1248, 1579, 2232
HphI GGTGA 4 cut(s) 130, 881, 1052, 1535
Hpy166II GTNNAC 8 cut(s) 205, 568, 983, 1178, 1245, 1607, 1880, 2239
Hpy8I GTNNAC 8 cut(s) 205, 568, 983, 1178, 1245, 1607, 1880, 2239
Hpy99I CGWCG 1 cut(s) 1871
HpyAV CCTTC 8 cut(s) 241, 322, 1306, 1313, 1563, 2041, 2063, 2178
HpyCH4III ACNGT 5 cut(s) 414, 572, 1037, 1242, 2026
HpyCH4IV ACGT 2 cut(s) 970, 1180
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 287, 1501, 1510
HpySE526I ACGT 2 cut(s) 970, 1180
Hsp92I GRCGYC 1 cut(s) 1550
HspAI GCGC 5 cut(s) 518, 610, 1450, 1550, 2149
KasI GGCGCC 1 cut(s) 1549
Kpn2I TCCGGA 2 cut(s) 197, 1578
Kzo9I GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
LmnI GCTCC 7 cut(s) 126, 184, 241, 904, 1064, 1394, 1647
MabI ACCWGGT 1 cut(s) 947
MaeI CTAG 7 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2307
MaeII ACGT 2 cut(s) 970, 1180
MaeIII GTNAC 7 cut(s) 430, 897, 1154, 1297, 1856, 2020, 2026
MalI GATC 7 cut(s) 191, 345, 526, 1218, 1574, 1893, 2281
MbiI CCGCTC 1 cut(s) 92
MboI GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
MboII GAAGA 8 cut(s) 370, 641, 794, 864, 1135, 1264, 1567, 1763
MfeI CAATTG 1 cut(s) 694
MflI RGATCY 4 cut(s) 343, 1216, 1572, 1891
MhlI GDGCHC 3 cut(s) 123, 223, 1052
Mly113I GGCGCC 1 cut(s) 1550
MlyI GAGTC 2 cut(s) 640, 733
MmeI TCCRAC 3 cut(s) 129, 707, 2257
MroI TCCGGA 2 cut(s) 197, 1578
MseI TTAA 4 cut(s) 537, 740, 2001, 2154
MslI CAYNNNNRTG 2 cut(s) 387, 2256
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 7 cut(s) 141, 146, 198, 1151, 1248, 1579, 2232
MunI CAATTG 1 cut(s) 694
Mva1269I GAATGC 1 cut(s) 480
MvaI CCWGG 9 cut(s) 399, 522, 933, 949, 1392, 1470, 1475, 1611, 2273
MvnI CGCG 2 cut(s) 850, 1452
MwoI GCNNNNNNNGC 4 cut(s) 56, 287, 1501, 1510
NarI GGCGCC 1 cut(s) 1550
NciI CCSGG 3 cut(s) 1152, 1249, 2232
NcoI CCATGG 2 cut(s) 2098, 2199
NdeI CATATG 1 cut(s) 1540
NdeII GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
NheI GCTAGC 1 cut(s) 1705
NlaIV GGNNCC 7 cut(s) 243, 678, 1378, 1551, 1643, 2118, 2260
NmeAIII GCCGAG 1 cut(s) 1169
NspI RCATGY 1 cut(s) 1344
PagI TCATGA 2 cut(s) 880, 2011
PasI CCCWGGG 1 cut(s) 398
PciI ACATGT 1 cut(s) 1340
PctI GAATGC 1 cut(s) 480
PfeI GAWTC 5 cut(s) 101, 271, 1359, 2092, 2284
PflMI CCANNNNNTGG 1 cut(s) 2205
PleI GAGTC 2 cut(s) 640, 732
PluTI GGCGCC 1 cut(s) 1553
PpsI GAGTC 2 cut(s) 640, 732
Ppu21I YACGTR 1 cut(s) 1181
PpuMI RGGWCCY 1 cut(s) 951
PscI ACATGT 1 cut(s) 1340
PshBI ATTAAT 1 cut(s) 740
Psp124BI GAGCTC 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 951
Psp6I CCWGG 9 cut(s) 397, 520, 931, 947, 1390, 1468, 1473, 1609, 2271
PspEI GGTNACC 1 cut(s) 1154
PspFI CCCAGC 1 cut(s) 1287
PspGI CCWGG 9 cut(s) 397, 520, 931, 947, 1390, 1468, 1473, 1609, 2271
PspN4I GGNNCC 7 cut(s) 243, 678, 1378, 1551, 1643, 2118, 2260
PspPI GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
PspPPI RGGWCCY 1 cut(s) 951
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 2 cut(s) 1082, 1113
PsuI RGATCY 4 cut(s) 343, 1216, 1572, 1891
RsaI GTAC 7 cut(s) 79, 984, 1177, 1458, 1608, 2074, 2182
RsaNI GTAC 7 cut(s) 78, 983, 1176, 1457, 1607, 2073, 2181
RseI CAYNNNNRTG 2 cut(s) 387, 2256
Rsr2I CGGWCCG 1 cut(s) 143
RsrII CGGWCCG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 223
SaqAI TTAA 4 cut(s) 537, 740, 2001, 2154
Sau3AI GATC 7 cut(s) 189, 343, 524, 1216, 1572, 1891, 2279
Sau96I GGNCC 8 cut(s) 143, 395, 677, 951, 1148, 1388, 1796, 1877
ScaI AGTACT 2 cut(s) 79, 2182
SchI GAGTC 2 cut(s) 640, 733
SduI GDGCHC 3 cut(s) 123, 223, 1052
SexAI ACCWGGT 1 cut(s) 947
SfcI CTRYAG 3 cut(s) 410, 600, 1842
SfoI GGCGCC 1 cut(s) 1551
SinI GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
SmiMI CAYNNNNRTG 2 cut(s) 387, 2256
SmlI CTYRAG 1 cut(s) 955
SmoI CTYRAG 1 cut(s) 955
SpeI ACTAGT 1 cut(s) 1670
SsiI CCGC 6 cut(s) 92, 150, 239, 848, 1639, 2223
SspDI GGCGCC 1 cut(s) 1549
SspI AATATT 1 cut(s) 1693
SspMI CTAG 7 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2307
SstI GAGCTC 1 cut(s) 223
StyI CCWWGG 2 cut(s) 2098, 2199
TaaI ACNGT 5 cut(s) 414, 572, 1037, 1242, 2026
TaiI ACGT 2 cut(s) 973, 1183
TaqI TCGA 3 cut(s) 633, 663, 2282
TatI WGTACW 5 cut(s) 77, 982, 1175, 1456, 2180
TauI GCSGC 3 cut(s) 152, 850, 1642
TfiI GAWTC 5 cut(s) 101, 271, 1359, 2092, 2284
Tru1I TTAA 4 cut(s) 537, 740, 2001, 2154
Tru9I TTAA 4 cut(s) 537, 740, 2001, 2154
TscAI CASTG 4 cut(s) 358, 687, 1387, 1609
TspDTI ATGAA 5 cut(s) 220, 1179, 1544, 1685, 2292
TspGWI ACGGA 3 cut(s) 197, 1634, 2081
TspRI CASTG 4 cut(s) 358, 687, 1387, 1609
Van91I CCANNNNNTGG 1 cut(s) 2205
VpaK11BI GGWCC 7 cut(s) 143, 677, 951, 1148, 1388, 1796, 1877
VspI ATTAAT 1 cut(s) 740
XapI RAATTY 5 cut(s) 21, 378, 1166, 1616, 2211
XceI RCATGY 1 cut(s) 1344
XcmI CCANNNNNNNNNTGG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 2238
XspI CTAG 7 cut(s) 579, 1005, 1061, 1671, 1706, 2184, 2307
ZrmI AGTACT 2 cut(s) 79, 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.