Prupe.6G063600_v2.0.a1

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0010845
Physical Location & Seq
Reverse (-)
4377387 .. 4383045
5659 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G063600.1

Sequence Viewer

Length: 2337 bp
ATGGAGTTTTTGGGTCTCGGCGATAACAAGGTTCGGAGAACTTATGGAGCTTTGTGGTTCAAAATCGTAATTCTACTCGTTCTGTGTAGTGTCGAAGGAGCTCTCTCCGATTCGAACTATTTGATTGGTCTTGGGAGCTACGACATCACTGGTCCTGCTGCTGATGTCAACATGATGGGGTACGCGAACACGGAGCAGATCGCTTCGGGGGTTCACTTCAGGTTGCGAGCTCGCACGTTCATTGTGGCCGAGCCGCAGGGGAACCGAGTGGCTTTTGTGAATCTTGATGCTTGCATGGCTTCACAGCTTGTGAAATTGAAAGTGGTGGAGAGGTTGAAGGCAAGGTATGGGGACCTGTACACTGAAAAGAATGTAGCTATTAGTGGAATTCACACCCACGCTGGCCCTGGGGGCTATCTCCAATATGTGGTGTATATTGTAACATCTCTTGGGTTTGTGCGTCAGTCATTTGATGTCCTTGTTGACGGCATTGCGAAAAGTATTATTCAAGCTCATGAAAATCTCGGGCCAGGATCAATTTTTGTTAATAAGGGGGAAATCTTAGATGCTGGCGTAAATCGCAGTCCTAGTGCTTATCTCAATAACCCTGCATCAGAACGCAGTAAATACAAGTATGACGTCGATAAAGAAATGACTCTTCTGAAGTTTGTTGATGATCAATGGGGTCCGGTTGGCAGCTTCAACTGGTTTGCAACTCATGGAACTTCTATGAGTCGTACAAACTCATTGATTAGTGGGGATAACAAGGGTGCTGCTGCTCGATTTATGGAAGACTGGTTTGAGGAGACTGGTTCTAGAAGTGCATATTCTGGTGAAGTTGCTGCTGATGGAATCCCGCGAAGAGTCTCAAACTTATTTAATGACCGTCATGATAATCACCATGAGTTACTAGAGCTTGCTGCATCCTTTCAGTCTCCTCCTGGTAAGCTAGCAACCAGGACCTTGAGTGTTGCAAGACGTGTCAGGGGTGCACTAAGGCAGGCTGACAAGCCTGGATTTGTTTCTGCATTTTGTCAATCAAACTGTGGTGATGTAAGCCCGAATGTTCTAGGCGCTTTCTGTACAGACACTGGACTACCTTGTGAGTTCAATCACAGCACCTGTGGTGGGAAAAATGAGTTGTGCTATGGCCGAGGACCGGGTTACCCAGATGAATTTGAAAGTACACGTATGATTGGTGAGAGGCAACTCAGAAAAGCTGTGGATCTTTTCAACAAAGCATCTGAGCAGCTGAAGGGGAAGGTTGACTATCGCCATGCCTACATAGACTTCTCCCAACTTGAAGTGACGCTTACCAAACAGGGAGGAGGTTCAAAGGTGGTAAAAACATGCCCTGCTGCAATGGGGTTTGGATTTGCTGCTGGAACCACTGACGGACCTGGAGCTTTTGATTTCACGCAAGGCGATGATAAGGGAAATGCCTTCTGGAGGTTGGTGCGCAATGTACTCAAAACACCAGGCAAGGAACAAGTGGATTGTCAGAATCCAAAGCCAATCTTACTTGATACTGGTGAAATGAAGCAACCATATGATTGGGCGCCTTCAATACTTCCAATTCAGATCATTCGAATAGGGCAGCTTGTCATTCTCAGTGTACCTGGAGAATTTACAACAATGGCTGGTAGGCGTCTCCGTGATGCTGTGAAGACAAAACTGACTAGTGGTAGCAATGGCGCAAATGTTCATGTTGTTATAGCAGGATTGACTAATACTTATTCACAGTATATAACTACCTTTGAAGAGTATCAGGTGCAGAGATATGAGGGTGCCTCCACTCTGTATGGACCACACACACTCAGTGCCTACATTCAGGAATTCAAGAAGCTTGCTACCGCTCTCATCAGTGGCAAACCTGTTGCACCGGGTCCACAACCCCCTGATCTCCTTGATAAACAAATAAGCTTACTTACACCTGTTGTGATGGATGCAACCCCTCGCGGTGTTAGTTTTGGGGACTGCAGCTCCGATGTTCCTCAGAACTCCACCTTCAAGAGAGGCCATGACATGGTAACAGTTACGTTCTGGTCAGCTTGTCCTCGGAATGACCTTATGACTGAAGGTACATTTGCCCTTGTGGAGATTCTCCACGGAAAGGATACTTGGGTTCCAGCTTATGACGACGATGATTTCTGCCTGCGGTTTAAGTGGTCAAGACCTTCCAAACTCAGCACTAGAAGTCAGGCAACCATAGAGTGGAGAATTCCTCAGTCTGCAACTCCCGGTGTGTACAGAATTAGACATTTTGGTGCCTCAAAGAGTCTGGTGGGATCGATTCGCCACTTTACAGGTTCATCTAGTGCTTTCGTGGTAGCATAA

Protein Analysis

779

Amino Acids

85.18

Weight (kDa)

8.39

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 642
Acc16I TGCGCA 1 cut(s) 1460
AccB1I GGYRCC 3 cut(s) 1558, 1787, 2267
AccB7I CCANNNNNTGG 3 cut(s) 427, 2026, 2214
AccBSI CCGCTC 1 cut(s) 1856
AccII CGCG 3 cut(s) 185, 859, 1959
AciI CCGC 5 cut(s) 254, 857, 1854, 1959, 2158
AclWI GGATC 3 cut(s) 541, 1233, 2296
AcoI YGGCCR 2 cut(s) 246, 1150
AcsI RAATTY 5 cut(s) 387, 1175, 1625, 1835, 2220
AcuI CTGAAG 4 cut(s) 202, 683, 1274, 2097
AcyI GRCGYC 3 cut(s) 639, 1559, 1648
AdeI CACNNNGTG 1 cut(s) 1820
AfaI GTAC 9 cut(s) 182, 359, 739, 1084, 1186, 1467, 1617, 2083, 2249
AfiI CCNNNNNNNGG 7 cut(s) 427, 1128, 1159, 1449, 2026, 2113, 2214
AflIII ACRYGT 2 cut(s) 979, 1187
AhlI ACTAGT 1 cut(s) 1679
AjiI CACGTC 1 cut(s) 980
AjnI CCWGG 8 cut(s) 406, 529, 940, 956, 1012, 1399, 1477, 1618
AjuI GAANNNNNNNTTGG 2 cut(s) 2104, 2136
Alw21I GWGCWC 3 cut(s) 103, 232, 994
Alw26I GTCTC 5 cut(s) 20, 800, 871, 939, 1655
Alw44I GTGCAC 1 cut(s) 990
AlwI GGATC 3 cut(s) 541, 1233, 2296
AlwNI CAGNNNCTG 2 cut(s) 1091, 1122
Ama87I CYCGRG 1 cut(s) 524
AoxI GGCC 5 cut(s) 246, 403, 527, 1150, 2017
ApaLI GTGCAC 1 cut(s) 990
ApoI RAATTY 5 cut(s) 387, 1175, 1625, 1835, 2220
AspLEI GCGC 4 cut(s) 1076, 1461, 1561, 1697
AsuC2I CCSGG 3 cut(s) 1161, 1884, 2241
AsuHPI GGTGA 5 cut(s) 845, 890, 1061, 1211, 1544
AsuII TTCGAA 2 cut(s) 113, 1588
AsuNHI GCTAGC 1 cut(s) 949
AvaI CYCGRG 1 cut(s) 524
AvaII GGWCC 8 cut(s) 152, 352, 686, 960, 1157, 1397, 1805, 1886
BaeGI GKGCMC 1 cut(s) 994
BaeI ACNNNNGTAYC 2 cut(s) 2109, 2142
BanI GGYRCC 3 cut(s) 1558, 1787, 2267
BanII GRGCYC 2 cut(s) 103, 232
BbsI GAAGAC 2 cut(s) 798, 1673
Bbv12I GWGCWC 3 cut(s) 103, 232, 994
BccI CCATC 3 cut(s) 169, 842, 1936
BceAI ACGGC 1 cut(s) 502
BciT130I CCWGG 8 cut(s) 408, 531, 942, 958, 1014, 1401, 1479, 1620
BciVI GTATCC 1 cut(s) 2110
BclI TGATCA 1 cut(s) 676
BcnI CCSGG 3 cut(s) 1161, 1884, 2241
BcoDI GTCTC 5 cut(s) 20, 800, 871, 939, 1655
BcuI ACTAGT 1 cut(s) 1679
BfaI CTAG 8 cut(s) 588, 816, 911, 950, 1070, 1680, 2193, 2316
BfmI CTRYAG 1 cut(s) 1978
BfoI RGCGCY 2 cut(s) 1077, 1562
BfuI GTATCC 1 cut(s) 2110
BglI GCCNNNNNGGC 1 cut(s) 411
Bme18I GGWCC 8 cut(s) 152, 352, 686, 960, 1157, 1397, 1805, 1886
BmeT110I CYCGRG 1 cut(s) 524
BmgBI CACGTC 1 cut(s) 980
BmiI GGNNCC 9 cut(s) 263, 353, 687, 1387, 1560, 1789, 1887, 2127, 2269
BmsI GCATC 7 cut(s) 277, 556, 620, 932, 1250, 1648, 1936
BmtI GCTAGC 1 cut(s) 953
BpiI GAAGAC 2 cut(s) 798, 1673
BplI GAGNNNNNCTC 4 cut(s) 1775, 1807, 2209, 2241
BpmI CTGGAG 3 cut(s) 1422, 1468, 1641
Bpu14I TTCGAA 2 cut(s) 113, 1588
BpuEI CTTGAG 1 cut(s) 985
BpuMI CCSGG 3 cut(s) 1161, 1884, 2241
Bsa29I ATCGAT 1 cut(s) 2291
BsaAI YACGTR 1 cut(s) 1190
BsaHI GRCGYC 3 cut(s) 639, 1559, 1648
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 5 cut(s) 406, 407, 1153, 2057, 2107
BsaWI WCCGGW 1 cut(s) 688
BsaXI ACNNNNNCTCC 2 cut(s) 1967, 1997
Bsc4I CCNNNNNNNGG 7 cut(s) 427, 1128, 1159, 1449, 2026, 2113, 2214
Bse1I ACTGG 6 cut(s) 154, 710, 800, 814, 1096, 1534
Bse3DI GCAATG 4 cut(s) 489, 1368, 1468, 1696
BseBI CCWGG 8 cut(s) 408, 531, 942, 958, 1014, 1401, 1479, 1620
BseCI ATCGAT 1 cut(s) 2291
BseDI CCNNGG 5 cut(s) 406, 407, 1153, 2057, 2107
BseGI GGATG 2 cut(s) 923, 1951
BseLI CCNNNNNNNGG 7 cut(s) 427, 1128, 1159, 1449, 2026, 2113, 2214
BseMI GCAATG 4 cut(s) 489, 1368, 1468, 1696
BseMII CTCAG 7 cut(s) 1225, 1236, 1624, 1831, 2009, 2200, 2240
BseNI ACTGG 6 cut(s) 154, 710, 800, 814, 1096, 1534
BseRI GAGGAG 3 cut(s) 818, 927, 1341
BseSI GKGCMC 1 cut(s) 994
BsgI GTGCAG 1 cut(s) 1793
Bsh1236I CGCG 3 cut(s) 185, 859, 1959
BshFI GGCC 5 cut(s) 248, 405, 529, 1152, 2019
BshNI GGYRCC 3 cut(s) 1558, 1787, 2267
BshVI ATCGAT 1 cut(s) 2291
BsiHKAI GWGCWC 3 cut(s) 103, 232, 994
BsiHKCI CYCGRG 1 cut(s) 524
BsiSI CCGG 4 cut(s) 689, 1160, 1883, 2241
BslFI GGGAC 2 cut(s) 365, 1988
BslI CCNNNNNNNGG 7 cut(s) 427, 1128, 1159, 1449, 2026, 2113, 2214
BsmAI GTCTC 5 cut(s) 20, 800, 871, 939, 1655
BsmBI CGTCTC 1 cut(s) 1655
BsmFI GGGAC 2 cut(s) 365, 1988
BsnI GGCC 5 cut(s) 248, 405, 529, 1152, 2019
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 524
Bsp119I TTCGAA 2 cut(s) 113, 1588
Bsp1286I GDGCHC 3 cut(s) 103, 232, 994
Bsp1407I TGTACA 3 cut(s) 357, 1082, 2247
Bsp143I GATC 7 cut(s) 198, 533, 676, 1225, 1581, 1900, 2288
BspACI CCGC 5 cut(s) 254, 857, 1854, 1959, 2158
BspANI GGCC 5 cut(s) 248, 405, 529, 1152, 2019
BspCNI CTCAG 7 cut(s) 1224, 1237, 1623, 1830, 2008, 2199, 2239
BspDI ATCGAT 1 cut(s) 2291
BspFNI CGCG 3 cut(s) 185, 859, 1959
BspHI TCATGA 2 cut(s) 514, 889
BspLI GGNNCC 9 cut(s) 263, 353, 687, 1387, 1560, 1789, 1887, 2127, 2269
BspMAI CTGCAG 1 cut(s) 1982
BspOI GCTAGC 1 cut(s) 953
BspPI GGATC 3 cut(s) 541, 1233, 2296
BspT104I TTCGAA 2 cut(s) 113, 1588
BspT107I GGYRCC 3 cut(s) 1558, 1787, 2267
BspTNI GGTCTC 1 cut(s) 20
BsrBI CCGCTC 1 cut(s) 1856
BsrDI GCAATG 4 cut(s) 489, 1368, 1468, 1696
BsrGI TGTACA 3 cut(s) 357, 1082, 2247
BsrI ACTGG 6 cut(s) 154, 710, 800, 814, 1096, 1534
BssECI CCNNGG 5 cut(s) 406, 407, 1153, 2057, 2107
BssMI GATC 7 cut(s) 198, 533, 676, 1225, 1581, 1900, 2288
BssNI GRCGYC 3 cut(s) 639, 1559, 1648
Bst2UI CCWGG 8 cut(s) 408, 531, 942, 958, 1014, 1401, 1479, 1620
Bst4CI ACNGT 4 cut(s) 887, 1046, 1743, 2035
Bst6I CTCTTC 3 cut(s) 663, 856, 1755
BstACI GRCGYC 3 cut(s) 639, 1559, 1648
BstAUI TGTACA 3 cut(s) 357, 1082, 2247
BstBAI YACGTR 1 cut(s) 1190
BstBI TTCGAA 2 cut(s) 113, 1588
BstDEI CTNAG 9 cut(s) 562, 995, 1211, 1245, 1610, 1817, 1995, 2186, 2226
BstDSI CCRYGG 1 cut(s) 2107
BstEII GGTNACC 1 cut(s) 1163
BstENI CCTNNNNNAGG 1 cut(s) 1447
BstF5I GGATG 2 cut(s) 923, 1951
BstFNI CGCG 3 cut(s) 185, 859, 1959
BstH2I RGCGCY 2 cut(s) 1077, 1562
BstHHI GCGC 4 cut(s) 1076, 1461, 1561, 1697
BstKTI GATC 7 cut(s) 201, 536, 679, 1228, 1584, 1903, 2291
BstMAI GTCTC 5 cut(s) 20, 800, 871, 939, 1655
BstMBI GATC 7 cut(s) 198, 533, 676, 1225, 1581, 1900, 2288
BstMWI GCNNNNNNNGC 3 cut(s) 296, 411, 579
BstNI CCWGG 8 cut(s) 408, 531, 942, 958, 1014, 1401, 1479, 1620
BstNSI RCATGY 1 cut(s) 1353
BstPI GGTNACC 1 cut(s) 1163
BstSFI CTRYAG 1 cut(s) 1978
BstSLI GKGCMC 1 cut(s) 994
BstUI CGCG 3 cut(s) 185, 859, 1959
BstV2I GAAGAC 2 cut(s) 798, 1673
BstX2I RGATCY 1 cut(s) 1225
BstXI CCANNNNNNTGG 1 cut(s) 1554
BstYI RGATCY 1 cut(s) 1225
Bsu15I ATCGAT 1 cut(s) 2291
BsuI GTATCC 1 cut(s) 2110
BsuRI GGCC 5 cut(s) 248, 405, 529, 1152, 2019
BsuTUI ATCGAT 1 cut(s) 2291
BtgI CCRYGG 1 cut(s) 2107
BtgZI GCGATG 1 cut(s) 1440
BtrI CACGTC 1 cut(s) 980
BtsCI GGATG 2 cut(s) 923, 1951
BtsIMutI CAGTG 7 cut(s) 147, 360, 1089, 1389, 1618, 1825, 1870
CaiI CAGNNNCTG 2 cut(s) 1091, 1122
CciI TCATGA 2 cut(s) 514, 889
CfoI GCGC 4 cut(s) 1076, 1461, 1561, 1697
ClaI ATCGAT 1 cut(s) 2291
CseI GACGC 3 cut(s) 449, 1318, 1637
Csp6I GTAC 9 cut(s) 181, 358, 738, 1083, 1185, 1466, 1616, 2082, 2248
CviQI GTAC 9 cut(s) 181, 358, 738, 1083, 1185, 1466, 1616, 2082, 2248
DdeI CTNAG 9 cut(s) 562, 995, 1211, 1245, 1610, 1817, 1995, 2186, 2226
DinI GGCGCC 1 cut(s) 1560
DpnI GATC 7 cut(s) 200, 535, 678, 1227, 1583, 1902, 2290
DpnII GATC 7 cut(s) 198, 533, 676, 1225, 1581, 1900, 2288
DraIII CACNNNGTG 1 cut(s) 1820
EaeI YGGCCR 2 cut(s) 246, 1150
Eam1104I CTCTTC 3 cut(s) 663, 856, 1755
EarI CTCTTC 3 cut(s) 663, 856, 1755
Ecl136II GAGCTC 2 cut(s) 101, 230
Eco24I GRGCYC 2 cut(s) 103, 232
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 8 cut(s) 152, 352, 686, 960, 1157, 1397, 1805, 1886
Eco53kI GAGCTC 2 cut(s) 101, 230
Eco57I CTGAAG 4 cut(s) 202, 683, 1274, 2097
Eco88I CYCGRG 1 cut(s) 524
Eco91I GGTNACC 1 cut(s) 1163
EcoICRI GAGCTC 2 cut(s) 101, 230
EcoNI CCTNNNNNAGG 1 cut(s) 1447
EcoO109I RGGNCCY 2 cut(s) 352, 960
EcoO65I GGTNACC 1 cut(s) 1163
EcoRI GAATTC 3 cut(s) 387, 1835, 2220
EcoRII CCWGG 8 cut(s) 406, 529, 940, 956, 1012, 1399, 1477, 1618
EcoT38I GRGCYC 2 cut(s) 103, 232
EgeI GGCGCC 1 cut(s) 1560
EheI GGCGCC 1 cut(s) 1560
Esp3I CGTCTC 1 cut(s) 1655
FalI AAGNNNNNCTT 4 cut(s) 1296, 1328, 1502, 1534
FaqI GGGAC 2 cut(s) 365, 1988
FauI CCCGC 1 cut(s) 864
FauNDI CATATG 1 cut(s) 1549
FbaI TGATCA 1 cut(s) 676
FokI GGATG 2 cut(s) 910, 1958
FriOI GRGCYC 2 cut(s) 103, 232
FspBI CTAG 8 cut(s) 588, 816, 911, 950, 1070, 1680, 2193, 2316
FspI TGCGCA 1 cut(s) 1460
GlaI GCGC 4 cut(s) 1075, 1460, 1560, 1696
GsuI CTGGAG 3 cut(s) 1422, 1468, 1641
HaeII RGCGCY 2 cut(s) 1077, 1562
HaeIII GGCC 5 cut(s) 248, 405, 529, 1152, 2019
HapII CCGG 4 cut(s) 689, 1160, 1883, 2241
HgaI GACGC 3 cut(s) 449, 1318, 1637
HhaI GCGC 4 cut(s) 1076, 1461, 1561, 1697
Hin1I GRCGYC 3 cut(s) 639, 1559, 1648
Hin6I GCGC 4 cut(s) 1074, 1459, 1559, 1695
HinP1I GCGC 4 cut(s) 1074, 1459, 1559, 1695
HincII GTYRAC 3 cut(s) 169, 484, 1267
HindII GTYRAC 3 cut(s) 169, 484, 1267
HindIII AAGCTT 2 cut(s) 1844, 1921
HpaII CCGG 4 cut(s) 689, 1160, 1883, 2241
HphI GGTGA 5 cut(s) 845, 890, 1061, 1211, 1544
Hpy188III TCNNGA 9 cut(s) 284, 515, 816, 890, 1447, 1832, 1840, 2011, 2172
Hpy99I CGWCG 2 cut(s) 644, 2144
HpyAV CCTTC 9 cut(s) 89, 331, 1249, 1255, 1453, 1572, 2017, 2072, 2187
HpyCH4III ACNGT 4 cut(s) 887, 1046, 1743, 2035
HpyCH4IV ACGT 5 cut(s) 236, 639, 979, 1189, 2039
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 411, 579
HpyF3I CTNAG 9 cut(s) 562, 995, 1211, 1245, 1610, 1817, 1995, 2186, 2226
HpySE526I ACGT 5 cut(s) 236, 639, 979, 1189, 2039
Hsp92I GRCGYC 3 cut(s) 639, 1559, 1648
HspAI GCGC 4 cut(s) 1074, 1459, 1559, 1695
KasI GGCGCC 1 cut(s) 1558
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 7 cut(s) 198, 533, 676, 1225, 1581, 1900, 2288
LmnI GCTCC 6 cut(s) 47, 98, 135, 193, 1403, 1988
LweI GCATC 7 cut(s) 277, 556, 620, 932, 1250, 1648, 1936
MaeI CTAG 8 cut(s) 588, 816, 911, 950, 1070, 1680, 2193, 2316
MaeII ACGT 5 cut(s) 236, 639, 979, 1189, 2039
MaeIII GTNAC 6 cut(s) 439, 906, 1163, 1306, 2029, 2035
MalI GATC 7 cut(s) 200, 535, 678, 1227, 1583, 1902, 2290
MbiI CCGCTC 1 cut(s) 1856
MboI GATC 7 cut(s) 198, 533, 676, 1225, 1581, 1900, 2288
MboII GAAGA 5 cut(s) 650, 803, 873, 1678, 1772
MflI RGATCY 1 cut(s) 1225
MhlI GDGCHC 3 cut(s) 103, 232, 994
Mly113I GGCGCC 1 cut(s) 1559
MlyI GAGTC 4 cut(s) 649, 742, 873, 2287
MseI TTAA 3 cut(s) 546, 879, 2163
MslI CAYNNNNRTG 1 cut(s) 2265
MspA1I CMGCKG 1 cut(s) 1252
MspI CCGG 4 cut(s) 689, 1160, 1883, 2241
MvaI CCWGG 8 cut(s) 408, 531, 942, 958, 1014, 1401, 1479, 1620
MvnI CGCG 3 cut(s) 185, 859, 1959
MwoI GCNNNNNNNGC 3 cut(s) 296, 411, 579
NarI GGCGCC 1 cut(s) 1559
NciI CCSGG 3 cut(s) 1161, 1884, 2241
NdeI CATATG 1 cut(s) 1549
NdeII GATC 7 cut(s) 198, 533, 676, 1225, 1581, 1900, 2288
NheI GCTAGC 1 cut(s) 949
NlaIV GGNNCC 9 cut(s) 263, 353, 687, 1387, 1560, 1789, 1887, 2127, 2269
NmeAIII GCCGAG 2 cut(s) 274, 1178
NmuCI GTSAC 1 cut(s) 1306
NsbI TGCGCA 1 cut(s) 1460
NspI RCATGY 1 cut(s) 1353
NspV TTCGAA 2 cut(s) 113, 1588
PagI TCATGA 2 cut(s) 514, 889
PasI CCCWGGG 1 cut(s) 407
PfeI GAWTC 6 cut(s) 110, 280, 852, 1504, 2101, 2293
PflMI CCANNNNNTGG 3 cut(s) 427, 2026, 2214
PleI GAGTC 4 cut(s) 649, 741, 872, 2286
PluTI GGCGCC 1 cut(s) 1562
PpsI GAGTC 4 cut(s) 649, 741, 872, 2286
Ppu21I YACGTR 1 cut(s) 1190
PpuMI RGGWCCY 2 cut(s) 352, 960
Psp124BI GAGCTC 2 cut(s) 103, 232
Psp5II RGGWCCY 2 cut(s) 352, 960
Psp6I CCWGG 8 cut(s) 406, 529, 940, 956, 1012, 1399, 1477, 1618
PspEI GGTNACC 1 cut(s) 1163
PspGI CCWGG 8 cut(s) 406, 529, 940, 956, 1012, 1399, 1477, 1618
PspN4I GGNNCC 9 cut(s) 263, 353, 687, 1387, 1560, 1789, 1887, 2127, 2269
PspPPI RGGWCCY 2 cut(s) 352, 960
PstI CTGCAG 1 cut(s) 1982
PstNI CAGNNNCTG 2 cut(s) 1091, 1122
PsuI RGATCY 1 cut(s) 1225
PvuII CAGCTG 1 cut(s) 1252
RsaI GTAC 9 cut(s) 182, 359, 739, 1084, 1186, 1467, 1617, 2083, 2249
RsaNI GTAC 9 cut(s) 181, 358, 738, 1083, 1185, 1466, 1616, 2082, 2248
RseI CAYNNNNRTG 1 cut(s) 2265
SacI GAGCTC 2 cut(s) 103, 232
SaqAI TTAA 3 cut(s) 546, 879, 2163
Sau3AI GATC 7 cut(s) 198, 533, 676, 1225, 1581, 1900, 2288
SchI GAGTC 4 cut(s) 649, 742, 873, 2287
SduI GDGCHC 3 cut(s) 103, 232, 994
SfaNI GCATC 7 cut(s) 277, 556, 620, 932, 1250, 1648, 1936
SfcI CTRYAG 1 cut(s) 1978
SfoI GGCGCC 1 cut(s) 1560
SfuI TTCGAA 2 cut(s) 113, 1588
SinI GGWCC 8 cut(s) 152, 352, 686, 960, 1157, 1397, 1805, 1886
SmiMI CAYNNNNRTG 1 cut(s) 2265
SmlI CTYRAG 1 cut(s) 964
SmoI CTYRAG 1 cut(s) 964
SpeI ACTAGT 1 cut(s) 1679
SsiI CCGC 5 cut(s) 254, 857, 1854, 1959, 2158
SspDI GGCGCC 1 cut(s) 1558
SspMI CTAG 8 cut(s) 588, 816, 911, 950, 1070, 1680, 2193, 2316
SstI GAGCTC 2 cut(s) 103, 232
TaaI ACNGT 4 cut(s) 887, 1046, 1743, 2035
TaiI ACGT 5 cut(s) 239, 642, 982, 1192, 2042
TaqI TCGA 6 cut(s) 93, 113, 642, 781, 1588, 2291
TatI WGTACW 5 cut(s) 357, 1082, 1184, 1465, 2247
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 6 cut(s) 110, 280, 852, 1504, 2101, 2293
Tru1I TTAA 3 cut(s) 546, 879, 2163
Tru9I TTAA 3 cut(s) 546, 879, 2163
TscAI CASTG 7 cut(s) 154, 367, 1096, 1396, 1618, 1825, 1870
TseFI GTSAC 1 cut(s) 1306
Tsp45I GTSAC 1 cut(s) 1306
TspDTI ATGAA 6 cut(s) 229, 531, 1188, 1553, 1694, 2301
TspGWI ACGGA 4 cut(s) 206, 1410, 1643, 2124
TspRI CASTG 7 cut(s) 154, 367, 1096, 1396, 1618, 1825, 1870
Van91I CCANNNNNTGG 3 cut(s) 427, 2026, 2214
VneI GTGCAC 1 cut(s) 990
VpaK11BI GGWCC 8 cut(s) 152, 352, 686, 960, 1157, 1397, 1805, 1886
XagI CCTNNNNNAGG 1 cut(s) 1447
XapI RAATTY 5 cut(s) 387, 1175, 1625, 1835, 2220
XbaI TCTAGA 1 cut(s) 815
XceI RCATGY 1 cut(s) 1353
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XspI CTAG 8 cut(s) 588, 816, 911, 950, 1070, 1680, 2193, 2316
ZraI GACGTC 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.