MD03G1079000.v1.1

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010845
Physical Location & Seq
Reverse (-)
6385893 .. 6391138
5246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1079000.v1.1.491

Sequence Viewer

Length: 2337 bp
ATGGAGTTTTTGGGTATCGCCAATTCCAAGGATACAGGAAAGTACAGAGGTTTGTGGGCTAAATTCGTAATTTTGATCCTTCTTTGTAGTAGCGGAGGAGCAGTCTCCGGTTCAGAGTATTTGATCGGTCTCGGGAGCTATGACATCACGGGGCCGGCCGCCGATGTCAACATGATGGGGTACGCGAACACGGAGCAGGTCGCTGCCGGAGTCCACTTCCGGTTGCGGGCTCGGACATTCATTGTGGCGGAGCCGCAAGGGAAGCGGGTGGCGTTTGTGAATCTCGATGCTTGCATGGCGTCGCAACTTGTGAAATTGAAGGTGGTCGAGAGGTTGAAGGCAAGGTATGGGGATTTATACACCGAAAAGAATGTAGCTATTAGTGGAATTCACACCCACGCTGGCCCTGGTGGTTATCTGCAATATGTGGTGTATATTGTGACATCTCTTGGATTCGTCCGTCAGTCATTTGATGTTCTTGTTGATGGAATTGAAAAAAGTATTACTCAAGCTCATGAAAATCTTGCGCCGGGATCAATTTTTGTTAATAAGGGGGAAATTTTAGACGCTGGTGTAAATCGCAGTCCTAGTGCTTATCTCAATAACCCTGCAGCAGAGCGCAGTATTTACAAGTATGATGTTGATAAAGATATGACTCTTCTGAAGTTTGTTGATGATCAATGGGGTCCAGTGGGTAGCTTTAATTGGTTTGCGACTCATGGAACTTCCATGAGTCGTAGTAACTCATTGATTAGTGGGGATAACAAGGGTGCTGCTGCACGATTTATGGAAGACTGGTTTGAAGAGAATGGTGCTAAAAGTGCATACTCTCATGAATTTGCTGCTGATGGAATCCCTCGAAGAGTCTCAAACTTACTTTATGACCGTCATGACAATCACCATGAGTTACTAGAGCTTGCTGCATCCTTTCAGTCTCGTCCTGGTAAGCCAGCAACCAGGACCTTGAGTGTTGCAAGACGTGTCAGGGGTGCACTAAGGCAGGCGGACAAGCCTCAATTTGTTTCTGCATTTTGTCAATCGAACTGTGGTGATGTAAGCCCCAATGTTCTAGGCGCTTTCTGTACAGACACTGGGTTACCTTGTGAATTTAATCACAGTACCTGTGGTGGGAAGAATGAGTTGTGCTATGGCCGTGGACCAGGCTACCCAGATGAATTTGAAAGTACACGTATAATTGGTGAGAGGCAACTCAAAAAAGCTGTGGATCTTTTCAACAAGGCATCTGAGAAGTTGAAGGGGAAAGTTGACTATCGTCATACTTATGTAGACTTCTCCCAACTTGAAGTAACCCTTCCCAAAAAGGGAGGAGGTTCGGAGGTGGTAAAAACATGTCCTGCTGCAATGGGGTTTGGATTTGCTGCTGGAACCACTGATGGACCTGGGGCTTTTGATTTCAAGCAAGGCGATGATAAGGGAAATCCATTCTGGAGGTTGGTGCGCAATGTACTTAAAAAGCCAGGCCAGGAACAAGTGGAATGTCAGAGTCCAAAGCCGATCTTGCTTGATACTGGTGAAATGAAGGAACCATATGACTGGGCGCCTTCAATACTTCCAATTCAGATCATCCGAGTAGGACAGCTTGTCATTCTCAGTGTACCTGGAGAATTTACAACAATGGCCGGTAGGCGCCTCCGTGATGCTGTGAAGTCAGTACTGACTAGTGGTGGTAATGGCGAAAATGTTCACGTTGTTATAGCTGGATTGACTAATACATATTCACAGTATATCACTACCTTTGAAGAGTATCAGGTGCAGAGATACGAGGGTGCCTCCACTCTGTATGGGCCGCACACACTCAGTGCCTACATTCAGGAGTTCAAGAAGCTTGCTACCGCTCTCACCAGTGGCAAATCCATTGCAGAAGGTCCACAACCCCCAGATCTCCTTGATAAACAAATAAGCTTACTTACACCAGTTGTGATGGATGGAACCCCTCGGGGCATTAGTTTTGGGGACTGCAGCTCCGATGTTCCTCAAAACTCCACCTTCAAGAAAGGCCATGACATGGTGACAGTTACCTTCTGGTCTGCTTGCCCTCGAAATGACCTTATGACTGAAGGTACATTCGCCCTTGTGGAAATTCTCCATGGAGAAGATACTTGGGTTCCAGCTTATGATGACGATGATTTCTGCCTGCGGTTTAAGTGGTCAAGACCCGCCAAACTTAGTACCAGAAGTCAGGCAACCATAGAATGGAGAATTCCTCAGTCTGCAACTCCCGGCGTATACAGAATTAGACATTTCGGTGCGTCAAAGAATTTCGTGGGATCCATTCGCCATTTTACAGGTTCATCTAGTGCATTCGTGGTAGCATAA

Protein Analysis

779

Amino Acids

85.23

Weight (kDa)

7.56

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase_alk PF04734 40 - 611 1.1e-230 Neutral/alkaline non-lysosomal ceramidase, N-terminal
Ceramidse_alk_C PF17048 613 - 777 2.8e-54 Neutral/alkaline non-lysosomal ceramidase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1460
Acc36I ACCTGC 1 cut(s) 187
AccB1I GGYRCC 3 cut(s) 1558, 1647, 1787
AccB7I CCANNNNNTGG 2 cut(s) 2026, 2214
AccBSI CCGCTC 1 cut(s) 1856
AccI GTMKAC 2 cut(s) 1287, 2247
AccII CGCG 1 cut(s) 185
AclWI GGATC 5 cut(s) 70, 541, 1233, 2283, 2296
AcoI YGGCCR 3 cut(s) 156, 1150, 1638
AcuI CTGAAG 2 cut(s) 683, 2097
AcyI GRCGYC 3 cut(s) 299, 1559, 1648
AdeI CACNNNGTG 1 cut(s) 1820
AfiI CCNNNNNNNGG 5 cut(s) 226, 1128, 1322, 2026, 2214
AflIII ACRYGT 3 cut(s) 979, 1187, 1349
AhdI GACNNNNNGTC 1 cut(s) 1601
AhlI ACTAGT 1 cut(s) 1679
AjiI CACGTC 1 cut(s) 980
AjnI CCWGG 8 cut(s) 406, 940, 956, 1159, 1399, 1477, 1482, 1618
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 4 cut(s) 109, 134, 871, 939
Alw44I GTGCAC 1 cut(s) 990
AlwI GGATC 5 cut(s) 70, 541, 1233, 2283, 2296
AlwNI CAGNNNCTG 2 cut(s) 1091, 1122
Ama87I CYCGRG 2 cut(s) 131, 1956
AoxI GGCC 8 cut(s) 152, 156, 403, 1150, 1480, 1638, 1805, 2017
ApaLI GTGCAC 1 cut(s) 990
ApeKI GCWGC 9 cut(s) 203, 611, 773, 776, 842, 920, 1358, 1379, 1980
Asp700I GAANNNNTTC 1 cut(s) 1701
AspLEI GCGC 6 cut(s) 529, 621, 1076, 1461, 1561, 1650
AspS9I GGNCC 8 cut(s) 152, 404, 686, 960, 1157, 1397, 1805, 1886
AsuC2I CCSGG 2 cut(s) 531, 2241
AsuHPI GGTGA 6 cut(s) 890, 1061, 1211, 1544, 1852, 2041
AvaI CYCGRG 2 cut(s) 131, 1956
AvaII GGWCC 5 cut(s) 686, 960, 1157, 1397, 1886
BaeGI GKGCMC 1 cut(s) 994
BaeI ACNNNNGTAYC 4 cut(s) 1771, 1804, 2109, 2142
BamHI GGATCC 1 cut(s) 2288
BanI GGYRCC 3 cut(s) 1558, 1647, 1787
BanII GRGCYC 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 1 cut(s) 994
BbvI GCAGC 9 cut(s) 190, 623, 760, 763, 829, 907, 1345, 1366, 1992
BccI CCATC 6 cut(s) 169, 479, 842, 1388, 1936, 1940
BceAI ACGGC 1 cut(s) 1137
BciT130I CCWGG 8 cut(s) 408, 942, 958, 1161, 1401, 1479, 1484, 1620
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 676
BcnI CCSGG 2 cut(s) 531, 2241
BcoDI GTCTC 4 cut(s) 109, 134, 871, 939
BcuI ACTAGT 1 cut(s) 1679
BfaI CTAG 5 cut(s) 588, 911, 1070, 1680, 2316
BfmI CTRYAG 2 cut(s) 609, 1978
BfoI RGCGCY 3 cut(s) 1077, 1562, 1651
BfuAI ACCTGC 1 cut(s) 187
BfuI GTATCC 1 cut(s) 25
BglII AGATCT 1 cut(s) 1900
BmcAI AGTACT 1 cut(s) 1674
Bme18I GGWCC 5 cut(s) 686, 960, 1157, 1397, 1886
BmeRI GACNNNNNGTC 1 cut(s) 1601
BmeT110I CYCGRG 2 cut(s) 131, 1956
BmgBI CACGTC 1 cut(s) 980
BmgT120I GGNCC 8 cut(s) 152, 404, 686, 960, 1157, 1397, 1805, 1886
BmrI ACTGGG 2 cut(s) 1101, 1564
BmsI GCATC 4 cut(s) 277, 932, 1250, 1648
BmuI ACTGGG 2 cut(s) 1101, 1564
BoxI GACNNNNGTC 1 cut(s) 1272
BpiI GAAGAC 1 cut(s) 798
BplI GAGNNNNNCTC 4 cut(s) 1775, 1807, 2209, 2241
BpmI CTGGAG 2 cut(s) 1468, 1641
BpuEI CTTGAG 2 cut(s) 492, 985
BpuMI CCSGG 2 cut(s) 531, 2241
BsaAI YACGTR 1 cut(s) 1190
BsaHI GRCGYC 3 cut(s) 299, 1559, 1648
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 6 cut(s) 27, 406, 1153, 1400, 1955, 2107
BsaWI WCCGGW 2 cut(s) 107, 219
BsaXI ACNNNNNCTCC 4 cut(s) 87, 117, 1967, 1997
Bsc4I CCNNNNNNNGG 5 cut(s) 226, 1128, 1322, 2026, 2214
Bse118I RCCGGY 2 cut(s) 154, 1640
Bse1I ACTGG 7 cut(s) 689, 800, 1096, 1534, 1559, 1863, 1934
Bse3DI GCAATG 3 cut(s) 1368, 1468, 1875
BseBI CCWGG 8 cut(s) 408, 942, 958, 1161, 1401, 1479, 1484, 1620
BseDI CCNNGG 6 cut(s) 27, 406, 1153, 1400, 1955, 2107
BseGI GGATG 3 cut(s) 923, 1584, 1951
BseLI CCNNNNNNNGG 5 cut(s) 226, 1128, 1322, 2026, 2214
BseMI GCAATG 3 cut(s) 1368, 1468, 1875
BseMII CTCAG 4 cut(s) 1236, 1624, 1831, 2240
BseNI ACTGG 7 cut(s) 689, 800, 1096, 1534, 1559, 1863, 1934
BseRI GAGGAG 2 cut(s) 111, 1341
BseSI GKGCMC 1 cut(s) 994
BseX3I CGGCCG 1 cut(s) 156
BseXI GCAGC 9 cut(s) 190, 623, 760, 763, 829, 907, 1345, 1366, 1992
BsgI GTGCAG 2 cut(s) 762, 1793
Bsh1236I CGCG 1 cut(s) 185
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 8 cut(s) 154, 158, 405, 1152, 1482, 1640, 1807, 2019
BshNI GGYRCC 3 cut(s) 1558, 1647, 1787
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 994
BsiHKCI CYCGRG 2 cut(s) 131, 1956
BsiSI CCGG 7 cut(s) 108, 155, 207, 220, 530, 1641, 2241
BslFI GGGAC 1 cut(s) 1988
BslI CCNNNNNNNGG 5 cut(s) 226, 1128, 1322, 2026, 2214
BsmAI GTCTC 4 cut(s) 109, 134, 871, 939
BsmFI GGGAC 1 cut(s) 1988
BsmI GAATGC 1 cut(s) 2321
BsnI GGCC 8 cut(s) 154, 158, 405, 1152, 1482, 1640, 1807, 2019
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 2 cut(s) 131, 1956
Bsp1286I GDGCHC 2 cut(s) 232, 994
Bsp1407I TGTACA 1 cut(s) 1082
Bsp143I GATC 9 cut(s) 75, 123, 533, 676, 1225, 1515, 1581, 1900, 2288
Bsp19I CCATGG 1 cut(s) 2107
BspANI GGCC 8 cut(s) 154, 158, 405, 1152, 1482, 1640, 1807, 2019
BspCNI CTCAG 4 cut(s) 1237, 1623, 1830, 2239
BspFNI CGCG 1 cut(s) 185
BspHI TCATGA 3 cut(s) 514, 832, 889
BspMAI CTGCAG 2 cut(s) 613, 1982
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 5 cut(s) 70, 541, 1233, 2283, 2296
BspT107I GGYRCC 3 cut(s) 1558, 1647, 1787
BspTNI GGTCTC 1 cut(s) 134
BsrBI CCGCTC 1 cut(s) 1856
BsrDI GCAATG 3 cut(s) 1368, 1468, 1875
BsrFI RCCGGY 2 cut(s) 154, 1640
BsrGI TGTACA 1 cut(s) 1082
BsrI ACTGG 7 cut(s) 689, 800, 1096, 1534, 1559, 1863, 1934
BssAI RCCGGY 2 cut(s) 154, 1640
BssECI CCNNGG 6 cut(s) 27, 406, 1153, 1400, 1955, 2107
BssMI GATC 9 cut(s) 75, 123, 533, 676, 1225, 1515, 1581, 1900, 2288
BssNAI GTATAC 1 cut(s) 2248
BssNI GRCGYC 3 cut(s) 299, 1559, 1648
BssT1I CCWWGG 2 cut(s) 27, 2107
Bst1107I GTATAC 1 cut(s) 2248
Bst2UI CCWGG 8 cut(s) 408, 942, 958, 1161, 1401, 1479, 1484, 1620
Bst4CI ACNGT 5 cut(s) 887, 1046, 1118, 1743, 2035
Bst6I CTCTTC 4 cut(s) 663, 798, 856, 1755
BstACI GRCGYC 3 cut(s) 299, 1559, 1648
BstAUI TGTACA 1 cut(s) 1082
BstBAI YACGTR 1 cut(s) 1190
BstDEI CTNAG 6 cut(s) 995, 1245, 1610, 1817, 2186, 2226
BstDSI CCRYGG 2 cut(s) 1153, 2107
BstEII GGTNACC 1 cut(s) 1095
BstF5I GGATG 3 cut(s) 923, 1584, 1951
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 3 cut(s) 1077, 1562, 1651
BstHHI GCGC 6 cut(s) 529, 621, 1076, 1461, 1561, 1650
BstKTI GATC 9 cut(s) 78, 126, 536, 679, 1228, 1518, 1584, 1903, 2291
BstMAI GTCTC 4 cut(s) 109, 134, 871, 939
BstMBI GATC 9 cut(s) 75, 123, 533, 676, 1225, 1515, 1581, 1900, 2288
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 4 cut(s) 262, 296, 821, 1519
BstNI CCWGG 8 cut(s) 408, 942, 958, 1161, 1401, 1479, 1484, 1620
BstNSI RCATGY 1 cut(s) 1353
BstPAI GACNNNNGTC 1 cut(s) 1272
BstPI GGTNACC 1 cut(s) 1095
BstSFI CTRYAG 2 cut(s) 609, 1978
BstSLI GKGCMC 1 cut(s) 994
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 9 cut(s) 190, 623, 760, 763, 829, 907, 1345, 1366, 1992
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 3 cut(s) 1225, 1900, 2288
BstXI CCANNNNNNTGG 1 cut(s) 1554
BstYI RGATCY 3 cut(s) 1225, 1900, 2288
BstZ17I GTATAC 1 cut(s) 2248
BstZI CGGCCG 1 cut(s) 156
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 8 cut(s) 154, 158, 405, 1152, 1482, 1640, 1807, 2019
BtgI CCRYGG 2 cut(s) 1153, 2107
BtgZI GCGATG 1 cut(s) 1440
BtrI CACGTC 1 cut(s) 980
BtsCI GGATG 3 cut(s) 923, 1584, 1951
BtsIMutI CAGTG 6 cut(s) 696, 1089, 1389, 1618, 1825, 1870
BveI ACCTGC 1 cut(s) 187
CaiI CAGNNNCTG 2 cut(s) 1091, 1122
CciI TCATGA 3 cut(s) 514, 832, 889
CfoI GCGC 6 cut(s) 529, 621, 1076, 1461, 1561, 1650
Cfr10I RCCGGY 2 cut(s) 154, 1640
Cfr13I GGNCC 8 cut(s) 152, 404, 686, 960, 1157, 1397, 1805, 1886
CseI GACGC 3 cut(s) 288, 575, 2259
DdeI CTNAG 6 cut(s) 995, 1245, 1610, 1817, 2186, 2226
DinI GGCGCC 2 cut(s) 1560, 1649
DpnI GATC 9 cut(s) 77, 125, 535, 678, 1227, 1517, 1583, 1902, 2290
DpnII GATC 9 cut(s) 75, 123, 533, 676, 1225, 1515, 1581, 1900, 2288
DraIII CACNNNGTG 1 cut(s) 1820
DriI GACNNNNNGTC 1 cut(s) 1601
EaeI YGGCCR 3 cut(s) 156, 1150, 1638
EagI CGGCCG 1 cut(s) 156
Eam1104I CTCTTC 4 cut(s) 663, 798, 856, 1755
Eam1105I GACNNNNNGTC 1 cut(s) 1601
EarI CTCTTC 4 cut(s) 663, 798, 856, 1755
EciI GGCGGA 2 cut(s) 263, 1019
EclXI CGGCCG 1 cut(s) 156
Eco130I CCWWGG 2 cut(s) 27, 2107
Eco24I GRGCYC 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 5 cut(s) 686, 960, 1157, 1397, 1886
Eco52I CGGCCG 1 cut(s) 156
Eco57I CTGAAG 2 cut(s) 683, 2097
Eco88I CYCGRG 2 cut(s) 131, 1956
Eco91I GGTNACC 1 cut(s) 1095
EcoO109I RGGNCCY 1 cut(s) 960
EcoO65I GGTNACC 1 cut(s) 1095
EcoRI GAATTC 2 cut(s) 387, 2220
EcoRII CCWGG 8 cut(s) 406, 940, 956, 1159, 1399, 1477, 1482, 1618
EcoT14I CCWWGG 2 cut(s) 27, 2107
EcoT38I GRGCYC 1 cut(s) 232
EgeI GGCGCC 2 cut(s) 1560, 1649
EheI GGCGCC 2 cut(s) 1560, 1649
ErhI CCWWGG 2 cut(s) 27, 2107
FalI AAGNNNNNCTT 4 cut(s) 1296, 1328, 1502, 1534
FaqI GGGAC 1 cut(s) 1988
FauI CCCGC 3 cut(s) 219, 258, 2185
FauNDI CATATG 1 cut(s) 1549
FbaI TGATCA 1 cut(s) 676
FblI GTMKAC 2 cut(s) 1287, 2247
FokI GGATG 3 cut(s) 910, 1571, 1958
FriOI GRGCYC 1 cut(s) 232
FseI GGCCGGCC 1 cut(s) 158
FspBI CTAG 5 cut(s) 588, 911, 1070, 1680, 2316
FspI TGCGCA 1 cut(s) 1460
GlaI GCGC 6 cut(s) 528, 620, 1075, 1460, 1560, 1649
GsuI CTGGAG 2 cut(s) 1468, 1641
HaeII RGCGCY 3 cut(s) 1077, 1562, 1651
HaeIII GGCC 8 cut(s) 154, 158, 405, 1152, 1482, 1640, 1807, 2019
HapII CCGG 7 cut(s) 108, 155, 207, 220, 530, 1641, 2241
HgaI GACGC 3 cut(s) 288, 575, 2259
HhaI GCGC 6 cut(s) 529, 621, 1076, 1461, 1561, 1650
Hin1I GRCGYC 3 cut(s) 299, 1559, 1648
Hin6I GCGC 6 cut(s) 527, 619, 1074, 1459, 1559, 1648
HinP1I GCGC 6 cut(s) 527, 619, 1074, 1459, 1559, 1648
HincII GTYRAC 2 cut(s) 169, 1267
HindII GTYRAC 2 cut(s) 169, 1267
HindIII AAGCTT 2 cut(s) 1844, 1921
HinfI GANTC 9 cut(s) 210, 280, 453, 655, 715, 733, 852, 864, 1504
HpaII CCGG 7 cut(s) 108, 155, 207, 220, 530, 1641, 2241
HphI GGTGA 6 cut(s) 890, 1061, 1211, 1544, 1852, 2041
Hpy188I TCNGA 9 cut(s) 115, 234, 663, 1246, 1336, 1503, 1581, 1589, 1987
Hpy99I CGWCG 1 cut(s) 304
HpyCH4III ACNGT 5 cut(s) 887, 1046, 1118, 1743, 2035
HpyCH4IV ACGT 3 cut(s) 979, 1189, 1707
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 296, 821, 1519
HpyF3I CTNAG 6 cut(s) 995, 1245, 1610, 1817, 2186, 2226
HpySE526I ACGT 3 cut(s) 979, 1189, 1707
Hsp92I GRCGYC 3 cut(s) 299, 1559, 1648
HspAI GCGC 6 cut(s) 527, 619, 1074, 1459, 1559, 1648
KasI GGCGCC 2 cut(s) 1558, 1647
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 9 cut(s) 75, 123, 533, 676, 1225, 1515, 1581, 1900, 2288
LmnI GCTCC 5 cut(s) 98, 135, 193, 250, 1988
Lsp1109I GCAGC 9 cut(s) 190, 623, 760, 763, 829, 907, 1345, 1366, 1992
LweI GCATC 4 cut(s) 277, 932, 1250, 1648
MaeI CTAG 5 cut(s) 588, 911, 1070, 1680, 2316
MaeII ACGT 3 cut(s) 979, 1189, 1707
MaeIII GTNAC 7 cut(s) 439, 740, 906, 1095, 1306, 2029, 2035
MalI GATC 9 cut(s) 77, 125, 535, 678, 1227, 1517, 1583, 1902, 2290
MbiI CCGCTC 1 cut(s) 1856
MboI GATC 9 cut(s) 75, 123, 533, 676, 1225, 1515, 1581, 1900, 2288
MboII GAAGA 7 cut(s) 650, 803, 815, 873, 1144, 1772, 2126
MflI RGATCY 3 cut(s) 1225, 1900, 2288
MhlI GDGCHC 2 cut(s) 232, 994
Mly113I GGCGCC 2 cut(s) 1559, 1648
MlyI GAGTC 6 cut(s) 219, 649, 709, 742, 873, 1513
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 1701
MseI TTAA 5 cut(s) 546, 702, 1110, 1470, 2163
MslI CAYNNNNRTG 2 cut(s) 1281, 2265
MspI CCGG 7 cut(s) 108, 155, 207, 220, 530, 1641, 2241
Mva1269I GAATGC 1 cut(s) 2321
MvaI CCWGG 8 cut(s) 408, 942, 958, 1161, 1401, 1479, 1484, 1620
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 4 cut(s) 262, 296, 821, 1519
NaeI GCCGGC 1 cut(s) 156
NarI GGCGCC 2 cut(s) 1559, 1648
NciI CCSGG 2 cut(s) 531, 2241
NcoI CCATGG 1 cut(s) 2107
NdeI CATATG 1 cut(s) 1549
NdeII GATC 9 cut(s) 75, 123, 533, 676, 1225, 1515, 1581, 1900, 2288
NgoMIV GCCGGC 1 cut(s) 154
NmuCI GTSAC 2 cut(s) 439, 2029
NsbI TGCGCA 1 cut(s) 1460
NspI RCATGY 1 cut(s) 1353
PagI TCATGA 3 cut(s) 514, 832, 889
PciI ACATGT 1 cut(s) 1349
PctI GAATGC 1 cut(s) 2321
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 1701
PfeI GAWTC 3 cut(s) 280, 453, 852
PflMI CCANNNNNTGG 2 cut(s) 2026, 2214
PleI GAGTC 6 cut(s) 218, 649, 709, 741, 872, 1512
PluTI GGCGCC 2 cut(s) 1562, 1651
PpsI GAGTC 6 cut(s) 218, 649, 709, 741, 872, 1512
Ppu21I YACGTR 1 cut(s) 1190
PpuMI RGGWCCY 1 cut(s) 960
PscI ACATGT 1 cut(s) 1349
PshAI GACNNNNGTC 1 cut(s) 1272
Psp5II RGGWCCY 1 cut(s) 960
Psp6I CCWGG 8 cut(s) 406, 940, 956, 1159, 1399, 1477, 1482, 1618
PspEI GGTNACC 1 cut(s) 1095
PspGI CCWGG 8 cut(s) 406, 940, 956, 1159, 1399, 1477, 1482, 1618
PspPI GGNCC 8 cut(s) 152, 404, 686, 960, 1157, 1397, 1805, 1886
PspPPI RGGWCCY 1 cut(s) 960
PstI CTGCAG 2 cut(s) 613, 1982
PstNI CAGNNNCTG 2 cut(s) 1091, 1122
PsuI RGATCY 3 cut(s) 1225, 1900, 2288
RigI GGCCGGCC 1 cut(s) 158
RseI CAYNNNNRTG 2 cut(s) 1281, 2265
SaqAI TTAA 5 cut(s) 546, 702, 1110, 1470, 2163
Sau3AI GATC 9 cut(s) 75, 123, 533, 676, 1225, 1515, 1581, 1900, 2288
Sau96I GGNCC 8 cut(s) 152, 404, 686, 960, 1157, 1397, 1805, 1886
ScaI AGTACT 1 cut(s) 1674
SchI GAGTC 6 cut(s) 219, 649, 709, 742, 873, 1513
SduI GDGCHC 2 cut(s) 232, 994
SfaNI GCATC 4 cut(s) 277, 932, 1250, 1648
SfcI CTRYAG 2 cut(s) 609, 1978
SfoI GGCGCC 2 cut(s) 1560, 1649
SinI GGWCC 5 cut(s) 686, 960, 1157, 1397, 1886
SmiMI CAYNNNNRTG 2 cut(s) 1281, 2265
SmlI CTYRAG 2 cut(s) 507, 964
SmoI CTYRAG 2 cut(s) 507, 964
SpeI ACTAGT 1 cut(s) 1679
SspDI GGCGCC 2 cut(s) 1558, 1647
SspMI CTAG 5 cut(s) 588, 911, 1070, 1680, 2316
StyI CCWWGG 2 cut(s) 27, 2107
TaaI ACNGT 5 cut(s) 887, 1046, 1118, 1743, 2035
TaiI ACGT 3 cut(s) 982, 1192, 1710
TaqI TCGA 5 cut(s) 285, 327, 859, 1040, 2059
TaqII GACCGA 1 cut(s) 116
TatI WGTACW 5 cut(s) 42, 1082, 1184, 1465, 1672
TauI GCSGC 3 cut(s) 161, 256, 1810
TfiI GAWTC 3 cut(s) 280, 453, 852
Tru1I TTAA 5 cut(s) 546, 702, 1110, 1470, 2163
Tru9I TTAA 5 cut(s) 546, 702, 1110, 1470, 2163
TscAI CASTG 6 cut(s) 696, 1096, 1396, 1618, 1825, 1870
TseFI GTSAC 2 cut(s) 439, 2029
TseI GCWGC 9 cut(s) 203, 611, 773, 776, 842, 920, 1358, 1379, 1980
Tsp45I GTSAC 2 cut(s) 439, 2029
TspDTI ATGAA 6 cut(s) 229, 531, 849, 1188, 1553, 2301
TspGWI ACGGA 3 cut(s) 206, 449, 1643
TspRI CASTG 6 cut(s) 696, 1096, 1396, 1618, 1825, 1870
Van91I CCANNNNNTGG 2 cut(s) 2026, 2214
VneI GTGCAC 1 cut(s) 990
VpaK11BI GGWCC 5 cut(s) 686, 960, 1157, 1397, 1886
XceI RCATGY 1 cut(s) 1353
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmiI GTMKAC 2 cut(s) 1287, 2247
XmnI GAANNNNTTC 1 cut(s) 1701
XspI CTAG 5 cut(s) 588, 911, 1070, 1680, 2316
ZrmI AGTACT 1 cut(s) 1674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.