pycom03g06300

Neutral ceramidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010845
Physical Location & Seq
Reverse (-)
4939125 .. 4944172
5048 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06300.9

Sequence Viewer

Length: 2589 bp
ATGCCCCGGAGAAGACCCGAGATCCAAGCCCATCAAAATCCATCCAGTCAAATACTTCACACGATCCACAACAATCCAACGATCCGTTGCACGGAGATTCCATGCACCCCAAAGGAACGCTCCCCTAAAAATCTATATTTAAGCATCCGTGTCTTCCCTTCCCCGCTACCAGCACCTTCCTTCGCTTTGACTCCATTACAAAACTGCAGGTTTGATCGAGCTGTAATCGGTTCTTTTGAGGAGAGAGCAGCAATGGAGATTTTGGGTATCGCCAATTCCAAGGTTACGGGAAAGTACAGAGGTTTGTGGGCTAAATTCGTAATTTTGATCCTTCTGTGTAGTAGCGGAGGAGCAGTCTCCGGTTCAGAGTATTTGATCGGTCTCGGGAGCTACGACATCACGGGGCCGGCCGCCGATGTCAACATGATGGGGTACGCGAACACGGAGCAGGTCGCTGCCGGAGTCCACTTCCGGTTGCGGGCTCGGACGTTCATTGTGGCGGAGCCGCAAGGGAACCGGGTGGCGTTTGTGAATCTCGATGCTTGCATGGCGTCGCAACTTGTGAAATTGAAGGTGGTCGAGAGGTTGAAAGCAAGGTATGGGGATTTATACACCGAAAAGAATGTAGCTATTAGTGGAATTCACACCCACGCTGGCCCGGGTGGTTATCTCCAATATGTGGTGTATATTGTGACATCTCTTGGATTCGTGCGTCAGTCGTTTGATGTTCTTGTTGATGGAATTGAAAAAAGTATTACTCAAGCTCATGAAAATCTCGCACCGGGATCAATTTTTGTTAATAAGGGGGAAATTTTGGACGCTGGTGTAAATCGCAGTCCCAGTGCTTATCTCAGTAACCCTGAAGCAGAGCGCAGTAAATACAAGTATGATGTCGATAAAGATATGACTCTTCTAAAGTTTGTTGATGATCAATGGGGTCCAGTGGGCAGCTTTAATTGGTTTGCGACTCATGGAACTTCCATGAGCCGTAGTAACTCATTGATTAGTGGGGATAACAAGGGTGCTGCTGCACGATTTATGGAAGACTGGTTTGAAGAGAATGGTGCTAAAAGTGCATACTCTCATGAATTTGCTGCCGATGGAATCCCTCGAAGAGTCTCAAACTTATTTTTTGACCGTCATGACAATCACCATGAGTTACTAGAGCTTGCTGCATCCTTTCAGTCTCGTCCTGGTAAGCCAGCAACCAGGACCTTGAGTGTTGCAAGACGTGTCAGGGGTGCACTAAGGCAGGCGGACAAGCCTCAATTTGTTTCTGCATTTTGTCAATCGAACTGTGGTGATGTAAGCCCCAATGTTCTAGGCGCTTTCTGTACAGACACTGGGTTACCTTGTGAATTTAATCACAGTACCTGTGGTGGGAAGAATGAGTTGTGCTATGGCCGTGGACCAGGCTACCCAGATGAATTTGAAAGTACACGTATAATTGGTGAGAGGCAACTCAAAAAAGCTGTGGATCTTTTCAACAAGGCATCTGAGAAGTTGAAGGGGAAAGTTGACTATCGTCATACTTATGTAGACTTCTCCCAACTTGAAGTAACGCTTCCCAAAAAGGGAGGAGGTTCGGAGGTGGTAAAAACATGTCCTGCTGCAATGGGGTTTGGATTTGCTGCTGGAACCACTGATGGACCTGGGGCTTTTGATTTCACGCAAGGCGATGATAAGGGAAATCCATTCTGGAGGTTGGTGCGCAATGTACTTAAAAAGCCAGGCAAGGAACAAGTGGAATGTCAGAGTCCAAAGCCAATCTTGCTTGATACTGGTGAAATGAAGGAACCATATGACTGGGCGCCTTCAATACTTCCAATTCAGATCATCCGAGTAGGACAGCTTGTCATTCTCAGTGTACCTGGAGAATTTACAACAATGGCCGGTAGGCGCCTCCGTGATGTTGTGAAGTCAGTACTGACTAGTGGTGGTAATGGCGAAAATGTTCACGTTGTTATAGCTGGATTGACTAATACATATTCACAGTATATCACTACCTTTGAAGAGTATCAGGTGCAGAGATACGAGGGTGCCTCCACTCTGTATGGGCCACACACGCTCAGTGCCTATATTCAGGAGTTCAAGAAGCTTGCTACCGCTCTCACCAGTGGCAAATCCATTGCAGAAGGTCCACAACCCCCAGATCTCCTTGATAAACAAATAAGCTTACTTACACCAGTTGTGATGGATGCAACCCCTCGTGGTGTTAGTTTTGGGGACTGCAGCTCCGATGTTCCTCAAAACTCCACCTTCAAGAGAGGCCATGACATGGTGACAGTTACCTTCTGGTCTGCTTGCCCTCGGAATGACCTTATGACTGAAGGTACATTCGCCCTTGTGGAAATTCTCCATGGAGAAGATACTTGGGTTCCAGCTTATGACGACGATGATTTCTGCCTGCGGTTTAAGTGGTCAAGACCCGCCAAACTTAGTACCAGAAGTCAGGCAACCATAGAATGGAGAATTCCTCAGTCTGCAACTCCCGGTGTATACAGAATTAGACATTTTGGTGCCTCAAAGAGTTTAGTGGGATCCATTCGCCATTTTACAGGTTCATCTAGTGCCTTTGTGGTAGCATAA

Protein Analysis

863

Amino Acids

94.74

Weight (kDa)

8.42

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010845)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07380 AT1G07380 AT5G58980
fragaria_vesca FvH4_3g37680
malus_domestica MD03G1079000.v1.1
prunus_persica Prupe.6G063600_v2.0.a1
pyrus_communis pycom03g06300
rosa_chinensis RchiOBHm_Chr5g0067951
rosa_laevigata RLG00000035946
rosa_multiflora Rmu_sc0000435.1_g000039
rosa_roxburghii Rroxscaffold_1G00012650 Rroxscaffold_1G00013360
rosa_rugosa Rorug05G0386600 Rorug05G0386700
rosa_samantha Rh5AG445600 Rh5BG463100 Rh5CG485000 Rh5DG477100
rosa_wichuraiana Rw0G015370 Rw5G041610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1712
Acc36I ACCTGC 2 cut(s) 198, 439
AccB1I GGYRCC 4 cut(s) 1810, 1899, 2039, 2519
AccB7I CCANNNNNTGG 3 cut(s) 679, 2278, 2466
AccBSI CCGCTC 1 cut(s) 2108
AccI GTMKAC 2 cut(s) 1539, 2499
AccII CGCG 1 cut(s) 437
AclWI GGATC 8 cut(s) 16, 58, 76, 322, 793, 1485, 2535, 2548
AcoI YGGCCR 3 cut(s) 408, 1402, 1890
AcsI RAATTY 9 cut(s) 314, 639, 810, 1088, 1358, 1427, 1877, 2352, 2472
AcuI CTGAAG 2 cut(s) 882, 2349
AcyI GRCGYC 3 cut(s) 551, 1811, 1900
AfiI CCNNNNNNNGG 7 cut(s) 91, 478, 679, 1380, 1574, 2278, 2466
AflIII ACRYGT 3 cut(s) 1231, 1439, 1601
AhdI GACNNNNNGTC 1 cut(s) 1853
AhlI ACTAGT 1 cut(s) 1931
AjiI CACGTC 1 cut(s) 1232
AjnI CCWGG 6 cut(s) 1192, 1208, 1411, 1651, 1729, 1870
Alw21I GWGCWC 1 cut(s) 1246
Alw26I GTCTC 4 cut(s) 361, 386, 1123, 1191
Alw44I GTGCAC 1 cut(s) 1242
AlwI GGATC 8 cut(s) 16, 58, 76, 322, 793, 1485, 2535, 2548
AlwNI CAGNNNCTG 2 cut(s) 1343, 1374
Ama87I CYCGRG 3 cut(s) 17, 383, 658
AoxI GGCC 7 cut(s) 404, 408, 655, 1402, 1890, 2057, 2269
ApaLI GTGCAC 1 cut(s) 1242
ApoI RAATTY 9 cut(s) 314, 639, 810, 1088, 1358, 1427, 1877, 2352, 2472
Asp700I GAANNNNTTC 1 cut(s) 1953
AspLEI GCGC 5 cut(s) 873, 1328, 1713, 1813, 1902
AspS9I GGNCC 8 cut(s) 404, 656, 938, 1212, 1409, 1649, 2057, 2138
AsuC2I CCSGG 6 cut(s) 7, 518, 659, 660, 783, 2493
AsuHPI GGTGA 6 cut(s) 1142, 1313, 1463, 1796, 2104, 2293
AvaI CYCGRG 3 cut(s) 17, 383, 658
AvaII GGWCC 5 cut(s) 938, 1212, 1409, 1649, 2138
BaeGI GKGCMC 1 cut(s) 1246
BaeI ACNNNNGTAYC 4 cut(s) 2023, 2056, 2361, 2394
BamHI GGATCC 1 cut(s) 2540
BanI GGYRCC 4 cut(s) 1810, 1899, 2039, 2519
BanII GRGCYC 1 cut(s) 484
BauI CACGAG 1 cut(s) 2208
BbsI GAAGAC 3 cut(s) 19, 145, 1050
Bbv12I GWGCWC 1 cut(s) 1246
BccI CCATC 7 cut(s) 39, 49, 421, 731, 1094, 1640, 2188
BceAI ACGGC 2 cut(s) 972, 1389
BciT130I CCWGG 6 cut(s) 1194, 1210, 1413, 1653, 1731, 1872
BclI TGATCA 1 cut(s) 928
BcnI CCSGG 6 cut(s) 7, 518, 659, 660, 783, 2493
BcoDI GTCTC 4 cut(s) 361, 386, 1123, 1191
BcuI ACTAGT 1 cut(s) 1931
BfaI CTAG 4 cut(s) 1163, 1322, 1932, 2568
BfmI CTRYAG 2 cut(s) 205, 2230
BfoI RGCGCY 3 cut(s) 1329, 1814, 1903
BfuAI ACCTGC 2 cut(s) 198, 439
BglII AGATCT 1 cut(s) 2152
BmcAI AGTACT 1 cut(s) 1926
Bme18I GGWCC 5 cut(s) 938, 1212, 1409, 1649, 2138
BmeRI GACNNNNNGTC 1 cut(s) 1853
BmeT110I CYCGRG 3 cut(s) 17, 383, 658
BmgBI CACGTC 1 cut(s) 1232
BmgT120I GGNCC 8 cut(s) 404, 656, 938, 1212, 1409, 1649, 2057, 2138
BmrI ACTGGG 3 cut(s) 834, 1353, 1816
BmsI GCATC 5 cut(s) 153, 529, 1184, 1502, 2188
BmuI ACTGGG 3 cut(s) 834, 1353, 1816
BoxI GACNNNNGTC 1 cut(s) 1524
BpiI GAAGAC 3 cut(s) 19, 145, 1050
BplI GAGNNNNNCTC 4 cut(s) 2027, 2059, 2461, 2493
BpmI CTGGAG 2 cut(s) 1720, 1893
BpuEI CTTGAG 2 cut(s) 744, 1237
BpuMI CCSGG 6 cut(s) 7, 518, 659, 660, 783, 2493
BsaAI YACGTR 1 cut(s) 1442
BsaHI GRCGYC 3 cut(s) 551, 1811, 1900
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 7 cut(s) 5, 279, 658, 1405, 1652, 2309, 2359
BsaWI WCCGGW 2 cut(s) 359, 471
BsaXI ACNNNNNCTCC 4 cut(s) 339, 369, 2219, 2249
Bsc4I CCNNNNNNNGG 7 cut(s) 91, 478, 679, 1380, 1574, 2278, 2466
Bse118I RCCGGY 2 cut(s) 406, 1892
Bse1I ACTGG 9 cut(s) 45, 840, 941, 1052, 1348, 1786, 1811, 2115, 2186
Bse3DI GCAATG 4 cut(s) 258, 1620, 1720, 2127
BseBI CCWGG 6 cut(s) 1194, 1210, 1413, 1653, 1731, 1872
BseDI CCNNGG 7 cut(s) 5, 279, 658, 1405, 1652, 2309, 2359
BseGI GGATG 5 cut(s) 41, 144, 1175, 1836, 2203
BseLI CCNNNNNNNGG 7 cut(s) 91, 478, 679, 1380, 1574, 2278, 2466
BseMI GCAATG 4 cut(s) 258, 1620, 1720, 2127
BseMII CTCAG 5 cut(s) 865, 1488, 1876, 2083, 2492
BseNI ACTGG 9 cut(s) 45, 840, 941, 1052, 1348, 1786, 1811, 2115, 2186
BseRI GAGGAG 3 cut(s) 254, 363, 1593
BseSI GKGCMC 1 cut(s) 1246
BseX3I CGGCCG 1 cut(s) 408
BsgI GTGCAG 2 cut(s) 1014, 2045
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 7 cut(s) 406, 410, 657, 1404, 1892, 2059, 2271
BshNI GGYRCC 4 cut(s) 1810, 1899, 2039, 2519
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 1246
BsiHKCI CYCGRG 3 cut(s) 17, 383, 658
BslFI GGGAC 2 cut(s) 822, 2240
BslI CCNNNNNNNGG 7 cut(s) 91, 478, 679, 1380, 1574, 2278, 2466
BsmAI GTCTC 4 cut(s) 361, 386, 1123, 1191
BsmFI GGGAC 2 cut(s) 822, 2240
BsnI GGCC 7 cut(s) 406, 410, 657, 1404, 1892, 2059, 2271
Bso31I GGTCTC 1 cut(s) 386
BsoBI CYCGRG 3 cut(s) 17, 383, 658
Bsp1286I GDGCHC 2 cut(s) 484, 1246
Bsp1407I TGTACA 1 cut(s) 1334
Bsp19I CCATGG 1 cut(s) 2359
BspANI GGCC 7 cut(s) 406, 410, 657, 1404, 1892, 2059, 2271
BspCNI CTCAG 5 cut(s) 864, 1489, 1875, 2082, 2491
BspFNI CGCG 1 cut(s) 437
BspHI TCATGA 3 cut(s) 766, 1084, 1141
BspMAI CTGCAG 2 cut(s) 209, 2234
BspMI ACCTGC 2 cut(s) 198, 439
BspPI GGATC 8 cut(s) 16, 58, 76, 322, 793, 1485, 2535, 2548
BspT107I GGYRCC 4 cut(s) 1810, 1899, 2039, 2519
BspTNI GGTCTC 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 2108
BsrDI GCAATG 4 cut(s) 258, 1620, 1720, 2127
BsrFI RCCGGY 2 cut(s) 406, 1892
BsrGI TGTACA 1 cut(s) 1334
BsrI ACTGG 9 cut(s) 45, 840, 941, 1052, 1348, 1786, 1811, 2115, 2186
BssAI RCCGGY 2 cut(s) 406, 1892
BssECI CCNNGG 7 cut(s) 5, 279, 658, 1405, 1652, 2309, 2359
BssNAI GTATAC 1 cut(s) 2500
BssNI GRCGYC 3 cut(s) 551, 1811, 1900
BssSI CACGAG 1 cut(s) 2208
BssT1I CCWWGG 2 cut(s) 279, 2359
Bst1107I GTATAC 1 cut(s) 2500
Bst2BI CACGAG 1 cut(s) 2208
Bst2UI CCWGG 6 cut(s) 1194, 1210, 1413, 1653, 1731, 1872
Bst4CI ACNGT 5 cut(s) 1139, 1298, 1370, 1995, 2287
Bst6I CTCTTC 4 cut(s) 915, 1050, 1108, 2007
BstACI GRCGYC 3 cut(s) 551, 1811, 1900
BstAUI TGTACA 1 cut(s) 1334
BstBAI YACGTR 1 cut(s) 1442
BstDEI CTNAG 7 cut(s) 851, 1247, 1497, 1862, 2069, 2438, 2478
BstDSI CCRYGG 2 cut(s) 1405, 2359
BstEII GGTNACC 1 cut(s) 1347
BstF5I GGATG 5 cut(s) 41, 144, 1175, 1836, 2203
BstFNI CGCG 1 cut(s) 437
BstH2I RGCGCY 3 cut(s) 1329, 1814, 1903
BstHHI GCGC 5 cut(s) 873, 1328, 1713, 1813, 1902
BstMAI GTCTC 4 cut(s) 361, 386, 1123, 1191
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 4 cut(s) 548, 1073, 1771, 2065
BstNI CCWGG 6 cut(s) 1194, 1210, 1413, 1653, 1731, 1872
BstNSI RCATGY 1 cut(s) 1605
BstPAI GACNNNNGTC 1 cut(s) 1524
BstPI GGTNACC 1 cut(s) 1347
BstSFI CTRYAG 2 cut(s) 205, 2230
BstSLI GKGCMC 1 cut(s) 1246
BstUI CGCG 1 cut(s) 437
BstV2I GAAGAC 3 cut(s) 19, 145, 1050
BstX2I RGATCY 4 cut(s) 21, 1477, 2152, 2540
BstXI CCANNNNNNTGG 1 cut(s) 1806
BstYI RGATCY 4 cut(s) 21, 1477, 2152, 2540
BstZ17I GTATAC 1 cut(s) 2500
BstZI CGGCCG 1 cut(s) 408
BsuRI GGCC 7 cut(s) 406, 410, 657, 1404, 1892, 2059, 2271
BtgI CCRYGG 2 cut(s) 1405, 2359
BtgZI GCGATG 1 cut(s) 1692
BtrI CACGTC 1 cut(s) 1232
BtsCI GGATG 5 cut(s) 41, 144, 1175, 1836, 2203
BtsIMutI CAGTG 7 cut(s) 847, 948, 1341, 1641, 1870, 2077, 2122
BveI ACCTGC 2 cut(s) 198, 439
CaiI CAGNNNCTG 2 cut(s) 1343, 1374
CciI TCATGA 3 cut(s) 766, 1084, 1141
CfoI GCGC 5 cut(s) 873, 1328, 1713, 1813, 1902
Cfr10I RCCGGY 2 cut(s) 406, 1892
Cfr13I GGNCC 8 cut(s) 404, 656, 938, 1212, 1409, 1649, 2057, 2138
Cfr9I CCCGGG 1 cut(s) 658
CseI GACGC 3 cut(s) 540, 701, 827
DdeI CTNAG 7 cut(s) 851, 1247, 1497, 1862, 2069, 2438, 2478
DinI GGCGCC 2 cut(s) 1812, 1901
DriI GACNNNNNGTC 1 cut(s) 1853
EaeI YGGCCR 3 cut(s) 408, 1402, 1890
EagI CGGCCG 1 cut(s) 408
Eam1104I CTCTTC 4 cut(s) 915, 1050, 1108, 2007
Eam1105I GACNNNNNGTC 1 cut(s) 1853
EarI CTCTTC 4 cut(s) 915, 1050, 1108, 2007
EciI GGCGGA 2 cut(s) 515, 1271
EclXI CGGCCG 1 cut(s) 408
Eco130I CCWWGG 2 cut(s) 279, 2359
Eco24I GRGCYC 1 cut(s) 484
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 5 cut(s) 938, 1212, 1409, 1649, 2138
Eco52I CGGCCG 1 cut(s) 408
Eco57I CTGAAG 2 cut(s) 882, 2349
Eco88I CYCGRG 3 cut(s) 17, 383, 658
Eco91I GGTNACC 1 cut(s) 1347
EcoO109I RGGNCCY 1 cut(s) 1212
EcoO65I GGTNACC 1 cut(s) 1347
EcoRI GAATTC 2 cut(s) 639, 2472
EcoRII CCWGG 6 cut(s) 1192, 1208, 1411, 1651, 1729, 1870
EcoT14I CCWWGG 2 cut(s) 279, 2359
EcoT38I GRGCYC 1 cut(s) 484
EgeI GGCGCC 2 cut(s) 1812, 1901
EheI GGCGCC 2 cut(s) 1812, 1901
ErhI CCWWGG 2 cut(s) 279, 2359
FalI AAGNNNNNCTT 4 cut(s) 1548, 1580, 1754, 1786
FaqI GGGAC 2 cut(s) 822, 2240
FauI CCCGC 3 cut(s) 171, 471, 2437
FauNDI CATATG 1 cut(s) 1801
FbaI TGATCA 1 cut(s) 928
FblI GTMKAC 2 cut(s) 1539, 2499
FokI GGATG 5 cut(s) 28, 131, 1162, 1823, 2210
FriOI GRGCYC 1 cut(s) 484
FseI GGCCGGCC 1 cut(s) 410
FspBI CTAG 4 cut(s) 1163, 1322, 1932, 2568
FspI TGCGCA 1 cut(s) 1712
GlaI GCGC 5 cut(s) 872, 1327, 1712, 1812, 1901
GsuI CTGGAG 2 cut(s) 1720, 1893
HaeII RGCGCY 3 cut(s) 1329, 1814, 1903
HaeIII GGCC 7 cut(s) 406, 410, 657, 1404, 1892, 2059, 2271
HgaI GACGC 3 cut(s) 540, 701, 827
HhaI GCGC 5 cut(s) 873, 1328, 1713, 1813, 1902
Hin1I GRCGYC 3 cut(s) 551, 1811, 1900
Hin6I GCGC 5 cut(s) 871, 1326, 1711, 1811, 1900
HinP1I GCGC 5 cut(s) 871, 1326, 1711, 1811, 1900
HincII GTYRAC 2 cut(s) 421, 1519
HindII GTYRAC 2 cut(s) 421, 1519
HindIII AAGCTT 2 cut(s) 2096, 2173
HphI GGTGA 6 cut(s) 1142, 1313, 1463, 1796, 2104, 2293
Hpy188I TCNGA 9 cut(s) 367, 486, 1498, 1588, 1755, 1833, 1841, 2239, 2313
Hpy99I CGWCG 2 cut(s) 556, 2396
HpyCH4III ACNGT 5 cut(s) 1139, 1298, 1370, 1995, 2287
HpyCH4IV ACGT 4 cut(s) 488, 1231, 1441, 1959
HpyF10VI GCNNNNNNNGC 4 cut(s) 548, 1073, 1771, 2065
HpyF3I CTNAG 7 cut(s) 851, 1247, 1497, 1862, 2069, 2438, 2478
HpySE526I ACGT 4 cut(s) 488, 1231, 1441, 1959
Hsp92I GRCGYC 3 cut(s) 551, 1811, 1900
HspAI GCGC 5 cut(s) 871, 1326, 1711, 1811, 1900
KasI GGCGCC 2 cut(s) 1810, 1899
KroI GCCGGC 1 cut(s) 406
KroNI GCCGGC 1 cut(s) 408
Ksp22I TGATCA 1 cut(s) 928
LmnI GCTCC 6 cut(s) 125, 350, 387, 445, 502, 2240
LweI GCATC 5 cut(s) 153, 529, 1184, 1502, 2188
MaeI CTAG 4 cut(s) 1163, 1322, 1932, 2568
MaeII ACGT 4 cut(s) 488, 1231, 1441, 1959
MaeIII GTNAC 9 cut(s) 283, 691, 854, 992, 1158, 1347, 1558, 2281, 2287
MbiI CCGCTC 1 cut(s) 2108
MboII GAAGA 9 cut(s) 24, 145, 902, 1055, 1067, 1125, 1396, 2024, 2378
MflI RGATCY 4 cut(s) 21, 1477, 2152, 2540
MhlI GDGCHC 2 cut(s) 484, 1246
Mly113I GGCGCC 2 cut(s) 1811, 1900
MlyI GAGTC 6 cut(s) 184, 471, 901, 961, 1125, 1765
MmeI TCCRAC 1 cut(s) 101
MroNI GCCGGC 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 1953
MseI TTAA 6 cut(s) 140, 798, 954, 1362, 1722, 2415
MslI CAYNNNNRTG 2 cut(s) 1533, 2517
MvaI CCWGG 6 cut(s) 1194, 1210, 1413, 1653, 1731, 1872
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 4 cut(s) 548, 1073, 1771, 2065
NaeI GCCGGC 1 cut(s) 408
NarI GGCGCC 2 cut(s) 1811, 1900
NciI CCSGG 6 cut(s) 7, 518, 659, 660, 783, 2493
NcoI CCATGG 1 cut(s) 2359
NdeI CATATG 1 cut(s) 1801
NgoMIV GCCGGC 1 cut(s) 406
NmuCI GTSAC 2 cut(s) 691, 2281
NsbI TGCGCA 1 cut(s) 1712
NspI RCATGY 1 cut(s) 1605
PagI TCATGA 3 cut(s) 766, 1084, 1141
PciI ACATGT 1 cut(s) 1601
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PdiI GCCGGC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 1953
PfeI GAWTC 4 cut(s) 97, 532, 705, 1104
PflMI CCANNNNNTGG 3 cut(s) 679, 2278, 2466
PleI GAGTC 6 cut(s) 184, 470, 901, 961, 1124, 1764
PluTI GGCGCC 2 cut(s) 1814, 1903
PpsI GAGTC 6 cut(s) 184, 470, 901, 961, 1124, 1764
Ppu21I YACGTR 1 cut(s) 1442
PpuMI RGGWCCY 1 cut(s) 1212
PscI ACATGT 1 cut(s) 1601
PshAI GACNNNNGTC 1 cut(s) 1524
Psp5II RGGWCCY 1 cut(s) 1212
Psp6I CCWGG 6 cut(s) 1192, 1208, 1411, 1651, 1729, 1870
PspEI GGTNACC 1 cut(s) 1347
PspGI CCWGG 6 cut(s) 1192, 1208, 1411, 1651, 1729, 1870
PspPI GGNCC 8 cut(s) 404, 656, 938, 1212, 1409, 1649, 2057, 2138
PspPPI RGGWCCY 1 cut(s) 1212
PstI CTGCAG 2 cut(s) 209, 2234
PstNI CAGNNNCTG 2 cut(s) 1343, 1374
PsuI RGATCY 4 cut(s) 21, 1477, 2152, 2540
RigI GGCCGGCC 1 cut(s) 410
RseI CAYNNNNRTG 2 cut(s) 1533, 2517
SaqAI TTAA 6 cut(s) 140, 798, 954, 1362, 1722, 2415
Sau96I GGNCC 8 cut(s) 404, 656, 938, 1212, 1409, 1649, 2057, 2138
ScaI AGTACT 1 cut(s) 1926
SchI GAGTC 6 cut(s) 184, 471, 901, 961, 1125, 1765
SduI GDGCHC 2 cut(s) 484, 1246
SfaNI GCATC 5 cut(s) 153, 529, 1184, 1502, 2188
SfcI CTRYAG 2 cut(s) 205, 2230
SfoI GGCGCC 2 cut(s) 1812, 1901
SinI GGWCC 5 cut(s) 938, 1212, 1409, 1649, 2138
SmaI CCCGGG 1 cut(s) 660
SmiMI CAYNNNNRTG 2 cut(s) 1533, 2517
SmlI CTYRAG 2 cut(s) 759, 1216
SmoI CTYRAG 2 cut(s) 759, 1216
SpeI ACTAGT 1 cut(s) 1931
SspDI GGCGCC 2 cut(s) 1810, 1899
SspMI CTAG 4 cut(s) 1163, 1322, 1932, 2568
StyI CCWWGG 2 cut(s) 279, 2359
TaaI ACNGT 5 cut(s) 1139, 1298, 1370, 1995, 2287
TaiI ACGT 4 cut(s) 491, 1234, 1444, 1962
TaqI TCGA 6 cut(s) 217, 537, 579, 894, 1111, 1292
TaqII GACCGA 1 cut(s) 368
TatI WGTACW 5 cut(s) 294, 1334, 1436, 1717, 1924
TauI GCSGC 2 cut(s) 413, 508
TfiI GAWTC 4 cut(s) 97, 532, 705, 1104
Tru1I TTAA 6 cut(s) 140, 798, 954, 1362, 1722, 2415
Tru9I TTAA 6 cut(s) 140, 798, 954, 1362, 1722, 2415
TscAI CASTG 7 cut(s) 847, 948, 1348, 1648, 1870, 2077, 2122
TseFI GTSAC 2 cut(s) 691, 2281
Tsp45I GTSAC 2 cut(s) 691, 2281
TspDTI ATGAA 6 cut(s) 481, 783, 1101, 1440, 1805, 2553
TspGWI ACGGA 5 cut(s) 74, 107, 137, 458, 1895
TspMI CCCGGG 1 cut(s) 658
TspRI CASTG 7 cut(s) 847, 948, 1348, 1648, 1870, 2077, 2122
Van91I CCANNNNNTGG 3 cut(s) 679, 2278, 2466
VneI GTGCAC 1 cut(s) 1242
VpaK11BI GGWCC 5 cut(s) 938, 1212, 1409, 1649, 2138
XapI RAATTY 9 cut(s) 314, 639, 810, 1088, 1358, 1427, 1877, 2352, 2472
XceI RCATGY 1 cut(s) 1605
XmaI CCCGGG 1 cut(s) 658
XmiI GTMKAC 2 cut(s) 1539, 2499
XmnI GAANNNNTTC 1 cut(s) 1953
XspI CTAG 4 cut(s) 1163, 1322, 1932, 2568
ZrmI AGTACT 1 cut(s) 1926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.