AT1G10150

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012893
Physical Location & Seq
Forward (+)
3325799 .. 3327614
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10150.1

Sequence Viewer

Length: 1245 bp
ATGGACCTTCTTCGATTGAGAGAAAAGGGTATATTTTTATCTCAGAGAAGAAGGAAATGGCTGATTTTTATGGCGATCTCTGGAGTTTCTGGCTATGGAGCTTACAAGGTTTATCATTTGCCATCTGTTGCCAGAAAAAGGAAGCGTCTTTTTAAGCTTTTCGGAGCCATTGTCTCTGTAGCTGAATTGATCTCTGATTCAGCTGAGACCTTAAGTATGGTATCACGAGACGTGAAGGATTTTCTCAATTCAGATTCAGATGAAATCCCTAACAGCTTGAAGCAGATCGCGAAGATCACAACTTCGAATGAGTTTACGGATTCGCTTTCTAGGGTTTCTCAGGCTGTGACTATTGGTGCCTTTCGTGGGTACAAATCCGAATCGTCTATTGGTGATTCAGGAATTGAGAAATCATCAGACTCGAGTGTTGTTGATAGAGTGATTGATAAGGTTTTCTCAGAGGCTGGAACTGGTTTTGTTTCAGTTGTTGTTGGTAGCTTTGCTAAGAATCTTGTTCTTGGATTTTACTCTGGTAAGGTAGAGAGTGGTGTGAAATGTGAGGGTTCTGATTCTTCTGAGACACCTAGATGGGTGACTTTGCTTGGTGATGACAAGTGTAGAGAGCTTTTAGCTGATTGTATTGAGAGATTCACCAGCACTGCAATTGGTGTGTATCTTGACAAGACGATGGATATCAATACTTATGATCAAATCTTTGAAGGCTTGACGAATCCGAAACATCAGGATAGTGTCAAGGACGTTCTTGTTTCGGTTTGTAACGGTGCTCTCGAGACTATTGTTAGGACATCTCACGACGTGTTTACATCTTCAAGGTCTAAGAATGTGATAGAAGAGATCGAAGATGATGATTTCAAGAGTAATGGTTCCGCGAGAAGCAAGATGGTTTCGGAATCAGGAGATGGGGTCAAGAGTAATGGGTGGACTGAGGCTATTGCAACTACATTGGCAGTTCCGAGCAATCGGAGGTTTATGTTTGATGTAACAGGAAGAGTAACACTAGAAACGACGAGATCAATCATCGCATTTATAATGGTGAAGACATTTCAAGGGTTCAGAAAAAGTATCAATGTGGTTCATGAAGAGGTTACAGACAGAGGACGCCAAGCAGTTGAATATGTTGGAGCCAAATCTTCTGTTATAATCACTGTATGCCTTGCGTTGTACTTGCACATCATAAGCGGCTGTGTTCGGAATTCTCCCATAGGCGTAAGCCAGCATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

414

Amino Acids

45.52

Weight (kDa)

7.6

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1049, 1160
AccB1I GGYRCC 1 cut(s) 356
AccII CGCG 2 cut(s) 290, 890
AciI CCGC 2 cut(s) 888, 1200
AcsI RAATTY 1 cut(s) 1213
AcyI GRCGYC 1 cut(s) 1120
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 2 cut(s) 371, 1184
AfiI CCNNNNNNNGG 2 cut(s) 138, 366
AflII CTTAAG 1 cut(s) 211
AflIII ACRYGT 1 cut(s) 816
AgsI TTSAA 6 cut(s) 280, 719, 831, 874, 1067, 1133
AjiI CACGTC 2 cut(s) 232, 817
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 8 cut(s) 101, 157, 182, 203, 276, 498, 625, 632
AluI AGCT 8 cut(s) 101, 157, 182, 203, 276, 498, 625, 632
Alw21I GWGCWC 1 cut(s) 787
Alw26I GTCTC 5 cut(s) 178, 200, 222, 572, 785
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 2 cut(s) 421, 788
ApoI RAATTY 1 cut(s) 1213
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 5 cut(s) 404, 604, 617, 643, 1066
AsuII TTCGAA 1 cut(s) 305
AvaI CYCGRG 2 cut(s) 421, 788
AvaII GGWCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 356
BauI CACGAG 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 1064
Bbv12I GWGCWC 1 cut(s) 787
BccI CCATC 5 cut(s) 130, 582, 682, 895, 914
BclI TGATCA 1 cut(s) 706
BcoDI GTCTC 5 cut(s) 178, 200, 222, 572, 785
BfaI CTAG 3 cut(s) 330, 585, 1019
BfmI CTRYAG 1 cut(s) 177
BfrI CTTAAG 1 cut(s) 211
BisI GCNGC 1 cut(s) 1201
BlsI GCNGC 1 cut(s) 1202
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 2 cut(s) 421, 788
BmgBI CACGTC 2 cut(s) 232, 817
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 4 cut(s) 166, 358, 886, 1144
BpiI GAAGAC 1 cut(s) 1064
BpmI CTGGAG 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 692
BsaHI GRCGYC 1 cut(s) 1120
BsaI GGTCTC 1 cut(s) 200
BsaXI ACNNNNNCTCC 4 cut(s) 156, 186, 909, 939
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 366
Bse1I ACTGG 1 cut(s) 475
Bse8I GATNNNNATC 1 cut(s) 692
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 2 cut(s) 138, 366
BseMII CTCAG 6 cut(s) 56, 195, 353, 471, 567, 936
BseNI ACTGG 1 cut(s) 475
Bsh1236I CGCG 2 cut(s) 290, 890
BshNI GGYRCC 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 787
BsiHKCI CYCGRG 2 cut(s) 421, 788
BslI CCNNNNNNNGG 2 cut(s) 138, 366
BsmAI GTCTC 5 cut(s) 178, 200, 222, 572, 785
BsmBI CGTCTC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 2 cut(s) 421, 788
Bsp119I TTCGAA 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 787
Bsp143I GATC 7 cut(s) 75, 189, 285, 294, 706, 855, 1031
Bsp68I TCGCGA 1 cut(s) 290
BspACI CCGC 2 cut(s) 888, 1200
BspCNI CTCAG 6 cut(s) 55, 196, 352, 470, 568, 937
BspFNI CGCG 2 cut(s) 290, 890
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 4 cut(s) 166, 358, 886, 1144
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 356
BspTI CTTAAG 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 200
BsrI ACTGG 1 cut(s) 475
BssMI GATC 7 cut(s) 75, 189, 285, 294, 706, 855, 1031
BssNI GRCGYC 1 cut(s) 1120
BssSI CACGAG 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 225
Bst4CI ACNGT 2 cut(s) 782, 1168
Bst6I CTCTTC 3 cut(s) 846, 1003, 1095
BstACI GRCGYC 1 cut(s) 1120
BstAFI CTTAAG 1 cut(s) 211
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 1235
BstDEI CTNAG 8 cut(s) 42, 204, 339, 457, 504, 576, 837, 945
BstFNI CGCG 2 cut(s) 290, 890
BstKTI GATC 7 cut(s) 78, 192, 288, 297, 709, 858, 1034
BstMAI GTCTC 5 cut(s) 178, 200, 222, 572, 785
BstMBI GATC 7 cut(s) 75, 189, 285, 294, 706, 855, 1031
BstSFI CTRYAG 1 cut(s) 177
BstUI CGCG 2 cut(s) 290, 890
BstV2I GAAGAC 1 cut(s) 1064
BtgZI GCGATG 1 cut(s) 1024
BtrI CACGTC 2 cut(s) 232, 817
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 2 cut(s) 657, 1164
BtuMI TCGCGA 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 1235
CaiI CAGNNNCTG 1 cut(s) 464
CciI TCATGA 1 cut(s) 1096
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 2 cut(s) 134, 1128
Csp6I GTAC 2 cut(s) 370, 1183
CviAII CATG 1 cut(s) 1097
CviQI GTAC 2 cut(s) 370, 1183
DdeI CTNAG 8 cut(s) 42, 204, 339, 457, 504, 576, 837, 945
DpnI GATC 7 cut(s) 77, 191, 287, 296, 708, 857, 1033
DpnII GATC 7 cut(s) 75, 189, 285, 294, 706, 855, 1031
DraIII CACNNNGTG 1 cut(s) 817
Eam1104I CTCTTC 3 cut(s) 846, 1003, 1095
EarI CTCTTC 3 cut(s) 846, 1003, 1095
Eco31I GGTCTC 1 cut(s) 200
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 1 cut(s) 4
Eco88I CYCGRG 2 cut(s) 421, 788
EcoRI GAATTC 1 cut(s) 1213
EcoRV GATATC 1 cut(s) 694
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 1 cut(s) 1100
FatI CATG 1 cut(s) 1096
FbaI TGATCA 1 cut(s) 706
Fnu4HI GCNGC 1 cut(s) 1201
Fsp4HI GCNGC 1 cut(s) 1201
FspBI CTAG 3 cut(s) 330, 585, 1019
GluI GCNGC 1 cut(s) 1201
GsuI CTGGAG 1 cut(s) 102
HgaI GACGC 2 cut(s) 134, 1128
Hin1I GRCGYC 1 cut(s) 1120
Hin1II CATG 1 cut(s) 1100
HindIII AAGCTT 1 cut(s) 155
HphI GGTGA 5 cut(s) 404, 604, 617, 643, 1066
Hpy166II GTNNAC 3 cut(s) 315, 822, 942
Hpy8I GTNNAC 3 cut(s) 315, 822, 942
Hpy99I CGWCG 2 cut(s) 818, 1030
HpyAV CCTTC 4 cut(s) 17, 45, 229, 713
HpyCH4III ACNGT 2 cut(s) 782, 1168
HpyCH4IV ACGT 3 cut(s) 231, 759, 816
HpyCH4V TGCA 3 cut(s) 662, 956, 1189
HpyF3I CTNAG 8 cut(s) 42, 204, 339, 457, 504, 576, 837, 945
HpySE526I ACGT 3 cut(s) 231, 759, 816
Hsp92I GRCGYC 1 cut(s) 1120
Hsp92II CATG 1 cut(s) 1100
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 7 cut(s) 75, 189, 285, 294, 706, 855, 1031
LmnI GCTCC 3 cut(s) 98, 164, 1142
MaeI CTAG 3 cut(s) 330, 585, 1019
MaeII ACGT 3 cut(s) 231, 759, 816
MaeIII GTNAC 6 cut(s) 346, 592, 776, 1000, 1012, 1105
MalI GATC 7 cut(s) 77, 191, 287, 296, 708, 857, 1033
MboI GATC 7 cut(s) 75, 189, 285, 294, 706, 855, 1031
MfeI CAATTG 1 cut(s) 663
MhlI GDGCHC 1 cut(s) 787
MluCI AATT 5 cut(s) 185, 247, 402, 663, 1213
MlyI GAGTC 1 cut(s) 413
MmeI TCCRAC 1 cut(s) 1120
MnlI CCTC 6 cut(s) 454, 553, 940, 978, 1096, 1109
MseI TTAA 2 cut(s) 153, 212
MslI CAYNNNNRTG 1 cut(s) 586
MspA1I CMGCKG 1 cut(s) 203
MspCI CTTAAG 1 cut(s) 211
MunI CAATTG 1 cut(s) 663
MvnI CGCG 2 cut(s) 290, 890
NdeII GATC 7 cut(s) 75, 189, 285, 294, 706, 855, 1031
NlaIII CATG 1 cut(s) 1100
NlaIV GGNNCC 4 cut(s) 166, 358, 886, 1144
NmuCI GTSAC 2 cut(s) 346, 592
NruI TCGCGA 1 cut(s) 290
NspV TTCGAA 1 cut(s) 305
PaeR7I CTCGAG 2 cut(s) 421, 788
PagI TCATGA 1 cut(s) 1096
PcsI WCGNNNNNNNCGW 1 cut(s) 786
PkrI GCNGC 1 cut(s) 1202
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
PsiI TTATAA 2 cut(s) 1049, 1160
PspN4I GGNNCC 4 cut(s) 166, 358, 886, 1144
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 464
PvuII CAGCTG 1 cut(s) 203
RruI TCGCGA 1 cut(s) 290
RsaI GTAC 2 cut(s) 371, 1184
RsaNI GTAC 2 cut(s) 370, 1183
RseI CAYNNNNRTG 1 cut(s) 586
SaqAI TTAA 2 cut(s) 153, 212
SatI GCNGC 1 cut(s) 1201
Sau3AI GATC 7 cut(s) 75, 189, 285, 294, 706, 855, 1031
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 413
SduI GDGCHC 1 cut(s) 787
SfcI CTRYAG 1 cut(s) 177
Sfr274I CTCGAG 2 cut(s) 421, 788
SfuI TTCGAA 1 cut(s) 305
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 2 cut(s) 421, 788
SmiMI CAYNNNNRTG 1 cut(s) 586
SmlI CTYRAG 3 cut(s) 211, 421, 788
SmoI CTYRAG 3 cut(s) 211, 421, 788
Sse9I AATT 5 cut(s) 185, 247, 402, 663, 1213
SsiI CCGC 2 cut(s) 888, 1200
SspMI CTAG 3 cut(s) 330, 585, 1019
TaaI ACNGT 2 cut(s) 782, 1168
TaiI ACGT 3 cut(s) 234, 762, 819
TaqI TCGA 5 cut(s) 13, 305, 422, 789, 858
TasI AATT 5 cut(s) 185, 247, 402, 663, 1213
TatI WGTACW 1 cut(s) 1182
TauI GCSGC 1 cut(s) 1203
Tru1I TTAA 2 cut(s) 153, 212
Tru9I TTAA 2 cut(s) 153, 212
TscAI CASTG 2 cut(s) 664, 1171
TseFI GTSAC 2 cut(s) 346, 592
Tsp45I GTSAC 2 cut(s) 346, 592
TspDTI ATGAA 3 cut(s) 276, 1085, 1113
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 2 cut(s) 664, 1171
Vha464I CTTAAG 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 1213
XhoI CTCGAG 2 cut(s) 421, 788
XspI CTAG 3 cut(s) 330, 585, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.