FvH4_2g32762

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012893
Physical Location & Seq
Reverse (-)
24754713 .. 24755957
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32762.t1

Sequence Viewer

Length: 1245 bp
ATGGAACCCGGGCTCGTCACGAAGGCCTCCCTCCTCTCTCCGCGCCGCAAGAAATGGCTTCTCATACTCGCGGCTCTCGGCGCGTCAGGTTACACCGCTTACAGGGTTTACAACTCGCCCTCTTTGGCCCGGAAGCGGAGGAGACTCACGAAGGTCGTCGGAGCCCTGATTTCCATGGCGGAGATGGTTTCTGATTCCGCCGAGACCACCGGCATTGTCTCTAAAGACTTGAAGGAGTTTCTGAGCTCCGATTCCGACGAAATGCCCAGAAGCCTGAAGCAGATTTCCAAGATTGCGAGGTCGGAGGAGTTCTCTGGGTCGTTGCAGAGGCTCACGGAGGCTTTAACGATCGGGGTTTACCGAGGGTTTGAGGCTGAGAAGAGGAATGGAGTCGAATCAGAGGAGAATACGGGGTTTTCGGAGAGAGTAATGGAGAAGGTCTTCTCTAAAGCCGGCACCGGGTTCGTTTCGGTTGTGGTTGGAAGCTTTGCGAGGAACATGGTTATGGGCTTTTACGACTCCGGTGAGAATTCGGGTGGAAATTCGAGTTCTTCTGCTTCGGATGTGCTTGATGTGGTTTGTAGCGATGGAAGTAAGGAGCTGATAGGGAATTGCATACAGGTTTTCGTGTCGACTGCGGTTGCGGCATACTTGGAGAAAACGAAGGATATCAATGTGTATGATGACTTGTTTGTTGGAATGACGAATCCCAAGCATGAGGAGAAGGTCCGGGAGCTTTTGGTTTCTGTATGTAATGGTGCTGTGGAGACTGTTGTGAAGACTTCTCATCAAGTTTTGACGAGTTCTGGTTCGAATTCGGAGTCAAATTTGGGTTCTGGGGCGGTTGTTGATGATAATGAAGGTGCGAGTGGAAGTAGGCATGAGTGTCTTGAGGAGGAAGCTTGTTCAATGCAGCCGAAGGAGGGAACATCATCGGAGGGTATTCAGGATAGCGGGTGGATTGGAAAGGTTTCGTCTACATTGGCAGTGCCGCAGAATAGGAAGTTTGTTCTTGACGTGACTGGGAGGGTGACATTCGAGACTGTAAGATCTTTTGTTCAGTTTATGATGTGGACGCTAATAGAGGGGTTGAGAAGAACTGTTAATATGGTTCATGGGGAGATTGTGGGTAGGGGGTTGCAAGTTATCAGATATTTTGGTGCAAAGTCTTCAGCTATTGTTACCATATGTCTTGCTTTGCATTTGTATGTCTTCAGTGGTACTAGAGTTCTCTTGACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

415

Amino Acids

44.8

Weight (kDa)

6.29

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 455
AccI GTMKAC 2 cut(s) 632, 977
AccII CGCG 3 cut(s) 43, 71, 83
AcsI RAATTY 4 cut(s) 529, 541, 814, 826
AcuI CTGAAG 3 cut(s) 296, 1155, 1198
AfaI GTAC 1 cut(s) 1222
AfiI CCNNNNNNNGG 4 cut(s) 102, 135, 459, 923
AgsI TTSAA 2 cut(s) 232, 909
AjiI CACGTC 1 cut(s) 1018
AluBI AGCT 6 cut(s) 246, 486, 601, 736, 902, 1175
AluI AGCT 6 cut(s) 246, 486, 601, 736, 902, 1175
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 5 cut(s) 136, 197, 223, 761, 1034
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 2 cut(s) 24, 126
ApeKI GCWGC 1 cut(s) 913
ApoI RAATTY 4 cut(s) 529, 541, 814, 826
ArsI GACNNNNNNTTYG 2 cut(s) 806, 838
Asp700I GAANNNNTTC 2 cut(s) 440, 970
AspLEI GCGC 2 cut(s) 45, 83
AspS9I GGNCC 2 cut(s) 127, 727
AsuC2I CCSGG 5 cut(s) 9, 10, 130, 460, 731
AsuHPI GGTGA 2 cut(s) 536, 1042
AsuII TTCGAA 1 cut(s) 812
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 727
BaeI ACNNNNGTAYC 2 cut(s) 1212, 1245
BanI GGYRCC 1 cut(s) 455
BanII GRGCYC 3 cut(s) 15, 166, 248
BbsI GAAGAC 4 cut(s) 433, 785, 1161, 1204
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 925
BccI CCATC 2 cut(s) 178, 581
BcgI CGANNNNNNTGC 2 cut(s) 445, 479
BcnI CCSGG 5 cut(s) 9, 10, 130, 460, 731
BcoDI GTCTC 5 cut(s) 136, 197, 223, 761, 1034
BfaI CTAG 1 cut(s) 1224
BglII AGATCT 1 cut(s) 1049
BisI GCNGC 5 cut(s) 46, 72, 645, 914, 992
BlsI GCNGC 5 cut(s) 47, 73, 646, 915, 993
Bme1390I CCNGG 5 cut(s) 9, 10, 130, 460, 731
Bme18I GGWCC 1 cut(s) 727
BmeT110I CYCGRG 1 cut(s) 8
BmgBI CACGTC 1 cut(s) 1018
BmgT120I GGNCC 2 cut(s) 127, 727
BmiI GGNNCC 3 cut(s) 6, 163, 457
BmrFI CCNGG 5 cut(s) 9, 10, 130, 460, 731
BmrI ACTGGG 1 cut(s) 1032
BmuI ACTGGG 1 cut(s) 1032
BpiI GAAGAC 4 cut(s) 433, 785, 1161, 1204
BplI GAGNNNNNCTC 2 cut(s) 296, 328
Bpu14I TTCGAA 1 cut(s) 812
BpuEI CTTGAG 1 cut(s) 911
BpuMI CCSGG 5 cut(s) 9, 10, 130, 460, 731
BsaI GGTCTC 1 cut(s) 197
BsaJI CCNNGG 3 cut(s) 8, 174, 361
BsaWI WCCGGW 1 cut(s) 521
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 135, 459, 923
Bse118I RCCGGY 2 cut(s) 209, 452
Bse1I ACTGG 1 cut(s) 1027
BseDI CCNNGG 3 cut(s) 8, 174, 361
BseGI GGATG 1 cut(s) 568
BseLI CCNNNNNNNGG 4 cut(s) 102, 135, 459, 923
BseMII CTCAG 2 cut(s) 233, 366
BseNI ACTGG 1 cut(s) 1027
BseRI GAGGAG 6 cut(s) 23, 154, 320, 416, 734, 908
BseXI GCAGC 1 cut(s) 925
Bsh1236I CGCG 3 cut(s) 43, 71, 83
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 2 cut(s) 26, 128
BshNI GGYRCC 1 cut(s) 455
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 248
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 7 cut(s) 9, 130, 210, 453, 459, 522, 730
BslI CCNNNNNNNGG 4 cut(s) 102, 135, 459, 923
BsmAI GTCTC 5 cut(s) 136, 197, 223, 761, 1034
BsnI GGCC 2 cut(s) 26, 128
Bso31I GGTCTC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 3 cut(s) 15, 166, 248
Bsp143I GATC 2 cut(s) 348, 1049
Bsp19I CCATGG 1 cut(s) 174
BspANI GGCC 2 cut(s) 26, 128
BspCNI CTCAG 2 cut(s) 234, 367
BspFNI CGCG 3 cut(s) 43, 71, 83
BspLI GGNNCC 3 cut(s) 6, 163, 457
BspT104I TTCGAA 1 cut(s) 812
BspT107I GGYRCC 1 cut(s) 455
BspTNI GGTCTC 1 cut(s) 197
BsrFI RCCGGY 2 cut(s) 209, 452
BsrI ACTGG 1 cut(s) 1027
BssAI RCCGGY 2 cut(s) 209, 452
BssECI CCNNGG 3 cut(s) 8, 174, 361
BssMI GATC 2 cut(s) 348, 1049
BssT1I CCWWGG 1 cut(s) 174
Bst4CI ACNGT 3 cut(s) 772, 1045, 1102
Bst6I CTCTTC 1 cut(s) 374
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 1 cut(s) 454
BstDEI CTNAG 3 cut(s) 242, 375, 1242
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 1 cut(s) 568
BstFNI CGCG 3 cut(s) 43, 71, 83
BstHHI GCGC 2 cut(s) 45, 83
BstKTI GATC 2 cut(s) 351, 1052
BstMAI GTCTC 5 cut(s) 136, 197, 223, 761, 1034
BstMBI GATC 2 cut(s) 348, 1049
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 2 cut(s) 80, 644
BstSCI CCNGG 5 cut(s) 7, 8, 128, 458, 729
BstUI CGCG 3 cut(s) 43, 71, 83
BstV1I GCAGC 1 cut(s) 925
BstV2I GAAGAC 4 cut(s) 433, 785, 1161, 1204
BstX2I RGATCY 1 cut(s) 1049
BstYI RGATCY 1 cut(s) 1049
BsuRI GGCC 2 cut(s) 26, 128
BtgI CCRYGG 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 600
BtrI CACGTC 1 cut(s) 1018
BtsCI GGATG 1 cut(s) 568
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 2 cut(s) 993, 1222
Cac8I GCNNGC 1 cut(s) 454
CfoI GCGC 2 cut(s) 45, 83
Cfr10I RCCGGY 2 cut(s) 209, 452
Cfr13I GGNCC 2 cut(s) 127, 727
Cfr9I CCCGGG 1 cut(s) 8
CseI GACGC 2 cut(s) 72, 1084
Csp6I GTAC 1 cut(s) 1221
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 5 cut(s) 175, 499, 716, 881, 1115
CviQI GTAC 1 cut(s) 1221
DdeI CTNAG 3 cut(s) 242, 375, 1242
DpnI GATC 2 cut(s) 350, 1051
DpnII GATC 2 cut(s) 348, 1049
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
EciI GGCGGA 2 cut(s) 187, 194
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 3 cut(s) 15, 166, 248
Eco31I GGTCTC 1 cut(s) 197
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 1 cut(s) 727
Eco53kI GAGCTC 1 cut(s) 246
Eco57I CTGAAG 3 cut(s) 296, 1155, 1198
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 1 cut(s) 246
EcoRI GAATTC 2 cut(s) 529, 814
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 174
EcoT38I GRGCYC 3 cut(s) 15, 166, 248
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 5 cut(s) 178, 502, 719, 884, 1118
FatI CATG 5 cut(s) 174, 498, 715, 880, 1114
FauI CCCGC 1 cut(s) 947
FauNDI CATATG 1 cut(s) 1187
FblI GTMKAC 2 cut(s) 632, 977
Fnu4HI GCNGC 5 cut(s) 46, 72, 645, 914, 992
FokI GGATG 1 cut(s) 575
FriOI GRGCYC 3 cut(s) 15, 166, 248
Fsp4HI GCNGC 5 cut(s) 46, 72, 645, 914, 992
FspBI CTAG 1 cut(s) 1224
GlaI GCGC 2 cut(s) 44, 82
GluI GCNGC 5 cut(s) 46, 72, 645, 914, 992
HaeIII GGCC 2 cut(s) 26, 128
HapII CCGG 7 cut(s) 9, 130, 210, 453, 459, 522, 730
HgaI GACGC 2 cut(s) 72, 1084
HhaI GCGC 2 cut(s) 45, 83
Hin1II CATG 5 cut(s) 178, 502, 719, 884, 1118
Hin6I GCGC 2 cut(s) 43, 81
HinP1I GCGC 2 cut(s) 43, 81
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HindIII AAGCTT 2 cut(s) 484, 900
HinfI GANTC 8 cut(s) 144, 194, 251, 390, 395, 518, 706, 821
HpaII CCGG 7 cut(s) 9, 130, 210, 453, 459, 522, 730
HphI GGTGA 2 cut(s) 536, 1042
Hpy166II GTNNAC 5 cut(s) 109, 358, 633, 978, 1074
Hpy188III TCNNGA 7 cut(s) 19, 148, 890, 947, 1013, 1039, 1234
Hpy8I GTNNAC 5 cut(s) 109, 358, 633, 978, 1074
Hpy99I CGWCG 2 cut(s) 161, 260
HpyAV CCTTC 8 cut(s) 16, 145, 226, 430, 658, 718, 854, 913
HpyCH4III ACNGT 3 cut(s) 772, 1045, 1102
HpyCH4IV ACGT 1 cut(s) 1017
HpyCH4V TGCA 6 cut(s) 325, 615, 913, 1141, 1163, 1201
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 644
HpyF3I CTNAG 3 cut(s) 242, 375, 1242
HpySE526I ACGT 1 cut(s) 1017
Hsp92II CATG 5 cut(s) 178, 502, 719, 884, 1118
HspAI GCGC 2 cut(s) 43, 81
KroI GCCGGC 1 cut(s) 452
KroNI GCCGGC 1 cut(s) 454
Kzo9I GATC 2 cut(s) 348, 1049
LmnI GCTCC 4 cut(s) 161, 251, 598, 733
Lsp1109I GCAGC 1 cut(s) 925
MaeI CTAG 1 cut(s) 1224
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 5 cut(s) 16, 89, 1018, 1030, 1180
MalI GATC 2 cut(s) 350, 1051
MboI GATC 2 cut(s) 348, 1049
MboII GAAGA 7 cut(s) 391, 433, 543, 790, 1107, 1161, 1204
MflI RGATCY 1 cut(s) 1049
MhlI GDGCHC 3 cut(s) 15, 166, 248
MluCI AATT 5 cut(s) 529, 541, 610, 814, 826
MlyI GAGTC 4 cut(s) 138, 399, 512, 830
MmeI TCCRAC 5 cut(s) 139, 279, 282, 460, 676
MroNI GCCGGC 1 cut(s) 452
MroXI GAANNNNTTC 2 cut(s) 440, 970
MseI TTAA 2 cut(s) 344, 1104
MslI CAYNNNNRTG 2 cut(s) 503, 1206
MspI CCGG 7 cut(s) 9, 130, 210, 453, 459, 522, 730
MspR9I CCNGG 5 cut(s) 9, 10, 130, 460, 731
MvnI CGCG 3 cut(s) 43, 71, 83
MwoI GCNNNNNNNGC 2 cut(s) 80, 644
NaeI GCCGGC 1 cut(s) 454
NciI CCSGG 5 cut(s) 9, 10, 130, 460, 731
NcoI CCATGG 1 cut(s) 174
NdeI CATATG 1 cut(s) 1187
NdeII GATC 2 cut(s) 348, 1049
NgoMIV GCCGGC 1 cut(s) 452
NlaIII CATG 5 cut(s) 178, 502, 719, 884, 1118
NlaIV GGNNCC 3 cut(s) 6, 163, 457
NmeAIII GCCGAG 2 cut(s) 57, 226
NmuCI GTSAC 3 cut(s) 16, 1018, 1030
NspV TTCGAA 1 cut(s) 812
PceI AGGCCT 1 cut(s) 26
PdiI GCCGGC 1 cut(s) 454
PdmI GAANNNNTTC 2 cut(s) 440, 970
PfeI GAWTC 4 cut(s) 194, 251, 395, 706
PfoI TCCNGGA 1 cut(s) 729
PkrI GCNGC 5 cut(s) 47, 73, 646, 915, 993
Ple19I CGATCG 1 cut(s) 351
PleI GAGTC 4 cut(s) 138, 398, 512, 829
PpsI GAGTC 4 cut(s) 138, 398, 512, 829
Psp124BI GAGCTC 1 cut(s) 248
PspN4I GGNNCC 3 cut(s) 6, 163, 457
PspPI GGNCC 2 cut(s) 127, 727
PsuI RGATCY 1 cut(s) 1049
PvuI CGATCG 1 cut(s) 351
RsaI GTAC 1 cut(s) 1222
RsaNI GTAC 1 cut(s) 1221
RseI CAYNNNNRTG 2 cut(s) 503, 1206
SacI GAGCTC 1 cut(s) 248
SalI GTCGAC 1 cut(s) 631
SaqAI TTAA 2 cut(s) 344, 1104
SatI GCNGC 5 cut(s) 46, 72, 645, 914, 992
Sau3AI GATC 2 cut(s) 348, 1049
Sau96I GGNCC 2 cut(s) 127, 727
SchI GAGTC 4 cut(s) 138, 399, 512, 830
ScrFI CCNGG 5 cut(s) 9, 10, 130, 460, 731
SduI GDGCHC 3 cut(s) 15, 166, 248
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 1 cut(s) 727
SmaI CCCGGG 1 cut(s) 10
SmiMI CAYNNNNRTG 2 cut(s) 503, 1206
SmlI CTYRAG 1 cut(s) 890
SmoI CTYRAG 1 cut(s) 890
Sse9I AATT 5 cut(s) 529, 541, 610, 814, 826
SseBI AGGCCT 1 cut(s) 26
SspMI CTAG 1 cut(s) 1224
SstI GAGCTC 1 cut(s) 248
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 5 cut(s) 7, 8, 128, 458, 729
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 3 cut(s) 772, 1045, 1102
TaiI ACGT 1 cut(s) 1020
TaqI TCGA 5 cut(s) 393, 545, 632, 812, 1038
TasI AATT 5 cut(s) 529, 541, 610, 814, 826
TauI GCSGC 4 cut(s) 48, 74, 647, 994
TfiI GAWTC 4 cut(s) 194, 251, 395, 706
Tru1I TTAA 2 cut(s) 344, 1104
Tru9I TTAA 2 cut(s) 344, 1104
TscAI CASTG 2 cut(s) 993, 1222
TseFI GTSAC 3 cut(s) 16, 1018, 1030
TseI GCWGC 1 cut(s) 913
Tsp45I GTSAC 3 cut(s) 16, 1018, 1030
TspDTI ATGAA 2 cut(s) 873, 1103
TspGWI ACGGA 1 cut(s) 350
TspMI CCCGGG 1 cut(s) 8
TspRI CASTG 2 cut(s) 993, 1222
VpaK11BI GGWCC 1 cut(s) 727
XapI RAATTY 4 cut(s) 529, 541, 814, 826
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmaI CCCGGG 1 cut(s) 8
XmiI GTMKAC 2 cut(s) 632, 977
XmnI GAANNNNTTC 2 cut(s) 440, 970
XspI CTAG 1 cut(s) 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.