MD15G1066400.v1.1

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012893
Physical Location & Seq
Reverse (-)
4616053 .. 4617321
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1066400.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGGAACTTGGGCTCGTCGCGAAGGGGTTGAATTTCTCTCGGAGAAGAAGGCGATGGCTTCTGATGCTCGCAGCTCTTGGGGTCTCCGGCTATGGAGCTTATAGGGTTTACAATTTGCCCTCTGTGGCCAGGAAGCGGAAGAGATGCCTGAAGCTCGTTGGAGCCCTAATCTCTATGGCCGAGACGGTCTCCGATTCGGCCGAGACGATCGGGGTTGTTTCCAAGGACTTGAAGGAGTTTTTGCGATCCGATTCGGATGAAATGCCCCAAAGTTTGAAGCAGATTTCGAAGATTGCTAGGTCGCAAGAGTTCTCCGTGTCACTGAGTAGGGTTACAGAGGCTATGACGGTTGGGATGATTCGAGGGTGTAGATTGGAGATGAACAAAGAGAGTGAATCGGAGAAGAGTATGGGGAATTCGAGCTTTTCAGATAGGGTTTTGGAGCAGGTTTTCTCTACAGCTGGAACCGGGTTTGTTTCGGTTATGGTTGGAAGCTTTGCTAAGAATTTGGTTCTGGGATTTTACTCCGATGAGCAATTGGGTGAGAATTCAAGTGTGTCGAAATCAAGTTCATCGGATGTGCCTAGATGGGTTAATGTGATTTGCAGTGACAGAAGTAAGGATCTCATAGCAAATTGCATTCAGGATTTTGTAAGCACTGCTGTTGCGGTTTACCTTGACAAAACGAAAGATATCAACGTTTATGAAGATATGTTTGATGGGTTGACCAATCCCAAGCATCAGAACGAAGTGAGGGATGTTCTAGTTTCTGTTTGTAATGGTGCTGTGGAAACAGTTGTGAGGACATCTCACCAAGTTTTGACGAGTTCCTGTTCGAGTTCAAGCTCGAATTCGCATGCATGTCCAATTGTTGATCAGAGTGAAGGTCCAAGTGGAACTGCGGATGAGTGCTTTCAGCGAGAGGTATCTCCAAAGCAGGGGAAGGTGGGGAGTTCGTCGGATGTTATTCATAGTAGTGGGTGGGTTAGGAAGGTTTCATCTACATTGGCTGTGCCGAGCAATAGGAAGTTTGTTCTGGATGTGACAGGGAGGGTGACGTTTGAGACGGTGCGATCTCTCGTTGAGTTTATGTTGTGGACGCTATTAGATGGCTTGAAAAGAAGCCTTAGTGCACTTCATGGGGAGGTTGCAGACAGGGGATTGCAAGTCATCAGATACTTCAGTGGGAAGTCTTCGGTTATAGTTACTGTATGTCTTGCTTTGCATTTATACGTCCTGGGTGGTTCAAGAGTTCTCTTGGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

423

Amino Acids

45.99

Weight (kDa)

8.77

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 436
AccII CGCG 1 cut(s) 20
AciI CCGC 3 cut(s) 136, 668, 902
AclI AACGTT 1 cut(s) 699
AclWI GGATC 2 cut(s) 240, 630
AcoI YGGCCR 3 cut(s) 126, 177, 198
AcsI RAATTY 5 cut(s) 31, 415, 505, 547, 850
AcuI CTGAAG 2 cut(s) 170, 1165
AfiI CCNNNNNNNGG 2 cut(s) 135, 938
AgsI TTSAA 7 cut(s) 31, 232, 277, 552, 843, 1117, 1248
AjnI CCWGG 2 cut(s) 128, 1236
AluBI AGCT 7 cut(s) 74, 98, 154, 423, 461, 495, 846
AluI AGCT 7 cut(s) 74, 98, 154, 423, 461, 495, 846
Alw21I GWGCWC 1 cut(s) 1135
Alw26I GTCTC 5 cut(s) 88, 176, 193, 197, 1058
Alw44I GTGCAC 1 cut(s) 1131
AlwI GGATC 2 cut(s) 240, 630
AoxI GGCC 3 cut(s) 126, 177, 198
ApaLI GTGCAC 1 cut(s) 1131
ApeKI GCWGC 2 cut(s) 71, 1262
ApoI RAATTY 5 cut(s) 31, 415, 505, 547, 850
AspS9I GGNCC 1 cut(s) 887
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 3 cut(s) 554, 803, 1066
AsuII TTCGAA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 887
BaeGI GKGCMC 1 cut(s) 1135
BaeI ACNNNNGTAYC 2 cut(s) 1168, 1201
BalI TGGCCA 1 cut(s) 128
BanII GRGCYC 2 cut(s) 15, 166
BbsI GAAGAC 1 cut(s) 1185
Bbv12I GWGCWC 1 cut(s) 1135
BbvI GCAGC 2 cut(s) 83, 1249
BccI CCATC 4 cut(s) 48, 582, 713, 1103
BciT130I CCWGG 2 cut(s) 130, 1238
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 5 cut(s) 88, 176, 193, 197, 1058
BfaI CTAG 3 cut(s) 297, 585, 764
BfmI CTRYAG 1 cut(s) 456
BfuAI ACCTGC 1 cut(s) 436
BisI GCNGC 2 cut(s) 72, 1263
BlsI GCNGC 2 cut(s) 73, 1264
Bme1390I CCNGG 3 cut(s) 130, 469, 1238
Bme18I GGWCC 1 cut(s) 887
BmgT120I GGNCC 1 cut(s) 887
BmiI GGNNCC 2 cut(s) 163, 466
BmrFI CCNGG 3 cut(s) 130, 469, 1238
BmsI GCATC 3 cut(s) 54, 134, 748
BpiI GAAGAC 1 cut(s) 1185
BplI GAGNNNNNCTC 4 cut(s) 173, 205, 793, 825
Bpu14I TTCGAA 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 469
BsaI GGTCTC 2 cut(s) 88, 193
BsaJI CCNNGG 2 cut(s) 222, 1237
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 938
BseBI CCWGG 2 cut(s) 130, 1238
BseDI CCNNGG 2 cut(s) 222, 1237
BseGI GGATG 7 cut(s) 262, 360, 583, 763, 910, 967, 1045
BseLI CCNNNNNNNGG 2 cut(s) 135, 938
BseMII CTCAG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 1135
BseX3I CGGCCG 1 cut(s) 198
BseXI GCAGC 2 cut(s) 83, 1249
Bsh1236I CGCG 1 cut(s) 20
Bsh1285I CGRYCG 2 cut(s) 201, 210
BshFI GGCC 3 cut(s) 128, 179, 200
BsiEI CGRYCG 2 cut(s) 201, 210
BsiHKAI GWGCWC 1 cut(s) 1135
BsiSI CCGG 2 cut(s) 87, 468
BslI CCNNNNNNNGG 2 cut(s) 135, 938
BsmAI GTCTC 5 cut(s) 88, 176, 193, 197, 1058
BsmBI CGTCTC 3 cut(s) 176, 197, 1058
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 3 cut(s) 128, 179, 200
Bso31I GGTCTC 2 cut(s) 88, 193
Bsp119I TTCGAA 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 15, 166, 1135
Bsp143I GATC 5 cut(s) 207, 245, 622, 874, 1073
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 3 cut(s) 136, 668, 902
BspANI GGCC 3 cut(s) 128, 179, 200
BspCNI CTCAG 1 cut(s) 315
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 2 cut(s) 163, 466
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 2 cut(s) 240, 630
BspT104I TTCGAA 1 cut(s) 287
BspTNI GGTCTC 2 cut(s) 88, 193
BssECI CCNNGG 2 cut(s) 222, 1237
BssMI GATC 5 cut(s) 207, 245, 622, 874, 1073
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 130, 1238
Bst4CI ACNGT 5 cut(s) 187, 349, 796, 1069, 1210
Bst6I CTCTTC 2 cut(s) 134, 398
BstBI TTCGAA 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 69, 858
BstDEI CTNAG 3 cut(s) 323, 501, 1127
BstF5I GGATG 7 cut(s) 262, 360, 583, 763, 910, 967, 1045
BstFNI CGCG 1 cut(s) 20
BstKTI GATC 5 cut(s) 210, 248, 625, 877, 1076
BstMAI GTCTC 5 cut(s) 88, 176, 193, 197, 1058
BstMBI GATC 5 cut(s) 207, 245, 622, 874, 1073
BstMCI CGRYCG 2 cut(s) 201, 210
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 2 cut(s) 130, 1238
BstNSI RCATGY 2 cut(s) 860, 864
BstSCI CCNGG 3 cut(s) 128, 467, 1236
BstSFI CTRYAG 1 cut(s) 456
BstSLI GKGCMC 1 cut(s) 1135
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 83, 1249
BstV2I GAAGAC 1 cut(s) 1185
BstX2I RGATCY 1 cut(s) 622
BstYI RGATCY 1 cut(s) 622
BstZI CGGCCG 1 cut(s) 198
BsuRI GGCC 3 cut(s) 128, 179, 200
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 7 cut(s) 262, 360, 583, 763, 910, 967, 1045
BtsI GCAGTG 2 cut(s) 613, 657
BtsIMutI CAGTG 4 cut(s) 320, 613, 657, 1189
BtuMI TCGCGA 1 cut(s) 20
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 2 cut(s) 69, 858
Cfr13I GGNCC 1 cut(s) 887
CseI GACGC 1 cut(s) 1108
CviAII CATG 3 cut(s) 857, 861, 1139
DdeI CTNAG 3 cut(s) 323, 501, 1127
DpnI GATC 5 cut(s) 209, 247, 624, 876, 1075
DpnII GATC 5 cut(s) 207, 245, 622, 874, 1073
EaeI YGGCCR 3 cut(s) 126, 177, 198
EagI CGGCCG 1 cut(s) 198
Eam1104I CTCTTC 2 cut(s) 134, 398
EarI CTCTTC 2 cut(s) 134, 398
EclXI CGGCCG 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 2 cut(s) 15, 166
Eco31I GGTCTC 2 cut(s) 88, 193
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 1 cut(s) 887
Eco52I CGGCCG 1 cut(s) 198
Eco57I CTGAAG 2 cut(s) 170, 1165
EcoRI GAATTC 3 cut(s) 415, 547, 850
EcoRII CCWGG 2 cut(s) 128, 1236
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 862
EcoT38I GRGCYC 2 cut(s) 15, 166
ErhI CCWWGG 1 cut(s) 222
Esp3I CGTCTC 3 cut(s) 176, 197, 1058
FaeI CATG 3 cut(s) 860, 864, 1142
FatI CATG 3 cut(s) 856, 860, 1138
FbaI TGATCA 1 cut(s) 874
Fnu4HI GCNGC 2 cut(s) 72, 1263
FokI GGATG 7 cut(s) 269, 367, 590, 770, 917, 974, 1052
FriOI GRGCYC 2 cut(s) 15, 166
Fsp4HI GCNGC 2 cut(s) 72, 1263
FspBI CTAG 3 cut(s) 297, 585, 764
GluI GCNGC 2 cut(s) 72, 1263
HaeIII GGCC 3 cut(s) 128, 179, 200
HapII CCGG 2 cut(s) 87, 468
HgaI GACGC 1 cut(s) 1108
Hin1II CATG 3 cut(s) 860, 864, 1142
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HindIII AAGCTT 1 cut(s) 493
HinfI GANTC 4 cut(s) 194, 251, 358, 395
HpaII CCGG 2 cut(s) 87, 468
HphI GGTGA 3 cut(s) 554, 803, 1066
Hpy166II GTNNAC 5 cut(s) 109, 673, 726, 1098, 1133
Hpy188III TCNNGA 4 cut(s) 19, 644, 1037, 1248
Hpy8I GTNNAC 5 cut(s) 109, 673, 726, 1098, 1133
Hpy99I CGWCG 2 cut(s) 20, 961
HpyAV CCTTC 6 cut(s) 16, 42, 226, 878, 937, 985
HpyCH4III ACNGT 5 cut(s) 187, 349, 796, 1069, 1210
HpyCH4IV ACGT 3 cut(s) 699, 1058, 1233
HpyCH4V TGCA 7 cut(s) 606, 639, 860, 1133, 1151, 1165, 1225
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpyF3I CTNAG 3 cut(s) 323, 501, 1127
HpySE526I ACGT 3 cut(s) 699, 1058, 1233
Hsp92II CATG 3 cut(s) 860, 864, 1142
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 5 cut(s) 207, 245, 622, 874, 1073
LmnI GCTCC 3 cut(s) 95, 161, 442
Lsp1109I GCAGC 2 cut(s) 83, 1249
LweI GCATC 3 cut(s) 54, 134, 748
MaeI CTAG 3 cut(s) 297, 585, 764
MaeII ACGT 3 cut(s) 699, 1058, 1233
MaeIII GTNAC 6 cut(s) 318, 331, 608, 1042, 1054, 1204
MalI GATC 5 cut(s) 209, 247, 624, 876, 1075
MboI GATC 5 cut(s) 207, 245, 622, 874, 1073
MboII GAAGA 6 cut(s) 57, 151, 301, 415, 719, 1185
MfeI CAATTG 2 cut(s) 536, 867
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 3 cut(s) 15, 166, 1135
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 9 cut(s) 31, 112, 415, 505, 536, 547, 634, 850, 867
MluNI TGGCCA 1 cut(s) 128
MmeI TCCRAC 3 cut(s) 139, 469, 939
MnlI CCTC 8 cut(s) 130, 331, 356, 747, 795, 916, 1044, 1138
Mox20I TGGCCA 1 cut(s) 128
Mph1103I ATGCAT 1 cut(s) 862
MscI TGGCCA 1 cut(s) 128
MseI TTAA 2 cut(s) 594, 1267
MslI CAYNNNNRTG 1 cut(s) 975
Msp20I TGGCCA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 2 cut(s) 87, 468
MspR9I CCNGG 3 cut(s) 130, 469, 1238
MunI CAATTG 2 cut(s) 536, 867
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 2 cut(s) 130, 1238
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 64
NciI CCSGG 1 cut(s) 469
NdeII GATC 5 cut(s) 207, 245, 622, 874, 1073
NlaIII CATG 3 cut(s) 860, 864, 1142
NlaIV GGNNCC 2 cut(s) 163, 466
NmeAIII GCCGAG 3 cut(s) 205, 226, 1041
NmuCI GTSAC 4 cut(s) 318, 608, 1042, 1054
NruI TCGCGA 1 cut(s) 20
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 2 cut(s) 860, 864
NspV TTCGAA 1 cut(s) 287
PaeI GCATGC 1 cut(s) 860
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PctI GAATGC 1 cut(s) 639
PfeI GAWTC 4 cut(s) 194, 251, 358, 395
PkrI GCNGC 2 cut(s) 73, 1264
Ple19I CGATCG 1 cut(s) 210
Psp1406I AACGTT 1 cut(s) 699
Psp6I CCWGG 2 cut(s) 128, 1236
PspGI CCWGG 2 cut(s) 128, 1236
PspN4I GGNNCC 2 cut(s) 163, 466
PspPI GGNCC 1 cut(s) 887
PsuI RGATCY 1 cut(s) 622
PvuI CGATCG 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 461
RruI TCGCGA 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 975
SaqAI TTAA 2 cut(s) 594, 1267
SatI GCNGC 2 cut(s) 72, 1263
Sau3AI GATC 5 cut(s) 207, 245, 622, 874, 1073
Sau96I GGNCC 1 cut(s) 887
ScrFI CCNGG 3 cut(s) 130, 469, 1238
SduI GDGCHC 3 cut(s) 15, 166, 1135
SfaNI GCATC 3 cut(s) 54, 134, 748
SfcI CTRYAG 1 cut(s) 456
SfuI TTCGAA 1 cut(s) 287
SinI GGWCC 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 975
SphI GCATGC 1 cut(s) 860
Sse9I AATT 9 cut(s) 31, 112, 415, 505, 536, 547, 634, 850, 867
SsiI CCGC 3 cut(s) 136, 668, 902
SspMI CTAG 3 cut(s) 297, 585, 764
StyD4I CCNGG 3 cut(s) 128, 467, 1236
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 5 cut(s) 187, 349, 796, 1069, 1210
TaiI ACGT 3 cut(s) 702, 1061, 1236
TaqI TCGA 6 cut(s) 287, 361, 419, 560, 836, 848
TasI AATT 9 cut(s) 31, 112, 415, 505, 536, 547, 634, 850, 867
TfiI GAWTC 4 cut(s) 194, 251, 358, 395
Tru1I TTAA 2 cut(s) 594, 1267
Tru9I TTAA 2 cut(s) 594, 1267
TscAI CASTG 4 cut(s) 327, 613, 664, 1189
TseFI GTSAC 4 cut(s) 318, 608, 1042, 1054
TseI GCWGC 2 cut(s) 71, 1262
Tsp45I GTSAC 4 cut(s) 318, 608, 1042, 1054
TspDTI ATGAA 7 cut(s) 273, 395, 561, 720, 959, 987, 1127
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 4 cut(s) 327, 613, 664, 1189
VneI GTGCAC 1 cut(s) 1131
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 5 cut(s) 31, 415, 505, 547, 850
XceI RCATGY 2 cut(s) 860, 864
XspI CTAG 3 cut(s) 297, 585, 764
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.