Rh6CG504000

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0012893
Physical Location & Seq
Forward (+)
66612939 .. 66614722
1784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG504000.1

Sequence Viewer

Length: 1209 bp
ATGGAACTCGGGCTCGTCACGAAGGCCTTGCATCGAAGAAAGAAATGGCTTCTGATACTCGCGGCCCTCGGCGCGTCCGGTTACGGCGCCTACAGGGTTTACAGTTCGCCCTCTTTGGTCCAGAAGCGAAAGAGGTTCATCAAGGTCGTCGGAGCCCTAATTTCTATGGCGGAGATGGCGTCTGATTCGGCCGAGACCGTCAGCATTGTCTCCAACGACTTGAAGGAGTTTCTGAACTCCGGTTCCGACGAAATGCCCAAAAGTCTGAAGCAGATTTCCAAGATTGCGAGGTCGGAGGAGTTCTCTGCGTCGTTGCAGAGGTTGACGGAGGCTATGACGATCGGGGTTTGCCGAGGGTTTGAGTTTGAGACGGGGAATGGGGTTGGATCGGAGAAGTCGGAGAGAGTTATGGAGAAGGTTTTCTCTGAAGCCGGGACCGGGTTCGTTTCGGTTGTAGTTGGAAGCTTTGCAAGGAATATGGTTATGGGGTTTTACTCAAATGAGCATTCAGGTCAGGATTCGAGTTCTTCGGATGTGAATAGATGGGTCGATGCGGTTTGTAGTGATCCAAGTAAGGAGCTGATAGCGAATTGCATGCAGGTTTTCGTGTCTACTGCGGTTGCGGCGTACCTAGACAAAACAAAGGATGTCAATTTCTGTGATGACATGTTTGGTGGAATGACCAATCCCAAGCATCAGGAGAAGGTCAGGGAGCTCTTGGTTTCTGTTTGCAATGGTGCTGTGGAGACTGTTGTGAAAACTTCTCATCAAGTTTTGACGAGTTCTGGTTCGAATCCGAGCTCAAATTCGGGGACTTGGTCAGTTGTTGAAGGTGTGAGTGGAGCTAGGGATGAGTGCCTTGAGCAAGAAGCAAAGCAGCCGAAGGAGGGAAGTTCACTTGGGGCTATTCAGAATAGCGAGTGGATTGGAAAGGTTTCGTCTACATTGGCAATGCCGCGCAATAGGAAGTTTGTTCGTGAGGTGACTGGGAGGGTGACATTCGAGACTGTAAGATCCTTTGTTGAATTCATGTTGTGGACACTACTGGAGGGGTTGAAAAGAACTGTTAGTGTGGTTCATGGGGAGATTGTGGGTAGGGGGTTGCAAGTTATCAGATATTTTGGGGCAAAGTCTTCGGCTATTGTTACCATGTGTCTTGCTTTGCACTTGTATGTTCTAGGAGGTAGTAGAGTTCTCTTGACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

43.7

Weight (kDa)

8.58

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 589
AccB1I GGYRCC 1 cut(s) 86
AccI GTMKAC 2 cut(s) 611, 941
AccII CGCG 3 cut(s) 62, 74, 958
AciI CCGC 6 cut(s) 62, 170, 554, 617, 623, 956
AclWI GGATC 3 cut(s) 394, 560, 1008
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 2 cut(s) 805, 1025
AcuI CTGAAG 2 cut(s) 287, 447
AcyI GRCGYC 2 cut(s) 87, 179
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 2 cut(s) 438, 887
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 4 cut(s) 223, 830, 1025, 1057
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 5 cut(s) 465, 580, 715, 801, 845
AluI AGCT 5 cut(s) 465, 580, 715, 801, 845
Alw21I GWGCWC 2 cut(s) 717, 803
Alw26I GTCTC 5 cut(s) 188, 214, 362, 740, 998
AlwI GGATC 3 cut(s) 394, 560, 1008
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 3 cut(s) 24, 63, 189
ApeKI GCWGC 1 cut(s) 877
ApoI RAATTY 2 cut(s) 805, 1025
Asp700I GAANNNNTTC 2 cut(s) 419, 934
AspLEI GCGC 3 cut(s) 74, 89, 960
AspS9I GGNCC 3 cut(s) 64, 118, 435
AsuC2I CCSGG 2 cut(s) 433, 439
AsuHPI GGTGA 2 cut(s) 994, 1006
AsuII TTCGAA 1 cut(s) 791
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 118, 435
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 4 cut(s) 15, 157, 717, 803
BbsI GAAGAC 1 cut(s) 1125
Bbv12I GWGCWC 2 cut(s) 717, 803
BbvI GCAGC 1 cut(s) 889
BccI CCATC 2 cut(s) 169, 537
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 6 cut(s) 10, 44, 577, 611, 1116, 1150
BcnI CCSGG 2 cut(s) 433, 439
BcoDI GTCTC 5 cut(s) 188, 214, 362, 740, 998
BfaI CTAG 3 cut(s) 632, 846, 1178
BfmI CTRYAG 1 cut(s) 91
BfoI RGCGCY 1 cut(s) 90
BfuAI ACCTGC 1 cut(s) 589
BisI GCNGC 4 cut(s) 63, 624, 878, 956
BlsI GCNGC 4 cut(s) 64, 625, 879, 957
Bme1390I CCNGG 2 cut(s) 433, 439
Bme18I GGWCC 2 cut(s) 118, 435
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 3 cut(s) 64, 118, 435
BmiI GGNNCC 4 cut(s) 88, 154, 244, 436
BmrFI CCNGG 2 cut(s) 433, 439
BmrI ACTGGG 1 cut(s) 996
BmsI GCATC 3 cut(s) 40, 541, 703
BmuI ACTGGG 1 cut(s) 996
BpiI GAAGAC 1 cut(s) 1125
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 1 cut(s) 1067
Bpu14I TTCGAA 1 cut(s) 791
BpuEI CTTGAG 1 cut(s) 881
BpuMI CCSGG 2 cut(s) 433, 439
BsaHI GRCGYC 2 cut(s) 87, 179
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 67, 352
BsaWI WCCGGW 2 cut(s) 77, 239
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 887
Bse1I ACTGG 2 cut(s) 991, 1050
Bse3DI GCAATG 2 cut(s) 739, 957
BseDI CCNNGG 2 cut(s) 67, 352
BseGI GGATG 3 cut(s) 538, 652, 856
BseLI CCNNNNNNNGG 2 cut(s) 438, 887
BseMI GCAATG 2 cut(s) 739, 957
BseNI ACTGG 2 cut(s) 991, 1050
BseRI GAGGAG 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 1 cut(s) 889
Bsh1236I CGCG 3 cut(s) 62, 74, 958
Bsh1285I CGRYCG 2 cut(s) 192, 342
BshFI GGCC 3 cut(s) 26, 65, 191
BshNI GGYRCC 1 cut(s) 86
BsiEI CGRYCG 2 cut(s) 192, 342
BsiHKAI GWGCWC 2 cut(s) 717, 803
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 4 cut(s) 78, 240, 432, 438
BslFI GGGAC 2 cut(s) 448, 826
BslI CCNNNNNNNGG 2 cut(s) 438, 887
BsmAI GTCTC 5 cut(s) 188, 214, 362, 740, 998
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 2 cut(s) 448, 826
BsmI GAATGC 1 cut(s) 505
BsnI GGCC 3 cut(s) 26, 65, 191
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 791
Bsp1286I GDGCHC 4 cut(s) 15, 157, 717, 803
Bsp143I GATC 4 cut(s) 339, 386, 565, 1013
BspACI CCGC 6 cut(s) 62, 170, 554, 617, 623, 956
BspANI GGCC 3 cut(s) 26, 65, 191
BspFNI CGCG 3 cut(s) 62, 74, 958
BspLI GGNNCC 4 cut(s) 88, 154, 244, 436
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 3 cut(s) 394, 560, 1008
BspT104I TTCGAA 1 cut(s) 791
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 739, 957
BsrI ACTGG 2 cut(s) 991, 1050
BssECI CCNNGG 2 cut(s) 67, 352
BssMI GATC 4 cut(s) 339, 386, 565, 1013
BssNI GRCGYC 2 cut(s) 87, 179
Bst4CI ACNGT 5 cut(s) 104, 199, 751, 1009, 1066
BstACI GRCGYC 2 cut(s) 87, 179
BstBI TTCGAA 1 cut(s) 791
BstC8I GCNNGC 1 cut(s) 596
BstDEI CTNAG 1 cut(s) 1206
BstF5I GGATG 3 cut(s) 538, 652, 856
BstFNI CGCG 3 cut(s) 62, 74, 958
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 74, 89, 960
BstKTI GATC 4 cut(s) 342, 389, 568, 1016
BstMAI GTCTC 5 cut(s) 188, 214, 362, 740, 998
BstMBI GATC 4 cut(s) 339, 386, 565, 1013
BstMCI CGRYCG 2 cut(s) 192, 342
BstMWI GCNNNNNNNGC 3 cut(s) 71, 176, 623
BstNSI RCATGY 2 cut(s) 598, 670
BstSCI CCNGG 2 cut(s) 431, 437
BstSFI CTRYAG 1 cut(s) 91
BstUI CGCG 3 cut(s) 62, 74, 958
BstV1I GCAGC 1 cut(s) 889
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 1013
BstZI CGGCCG 1 cut(s) 189
BsuRI GGCC 3 cut(s) 26, 65, 191
BtsCI GGATG 3 cut(s) 538, 652, 856
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 1 cut(s) 596
CfoI GCGC 3 cut(s) 74, 89, 960
Cfr13I GGNCC 3 cut(s) 64, 118, 435
CseI GACGC 3 cut(s) 63, 168, 297
Csp6I GTAC 1 cut(s) 628
CviAII CATG 5 cut(s) 595, 667, 1030, 1079, 1150
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 1 cut(s) 1206
DinI GGCGCC 1 cut(s) 88
DpnI GATC 4 cut(s) 341, 388, 567, 1015
DpnII GATC 4 cut(s) 339, 386, 565, 1013
EaeI YGGCCR 1 cut(s) 189
EagI CGGCCG 1 cut(s) 189
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 2 cut(s) 715, 801
EclXI CGGCCG 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 4 cut(s) 15, 157, 717, 803
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 118, 435
Eco52I CGGCCG 1 cut(s) 189
Eco53kI GAGCTC 2 cut(s) 715, 801
Eco57I CTGAAG 2 cut(s) 287, 447
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 715, 801
EcoRI GAATTC 1 cut(s) 1025
EcoT38I GRGCYC 4 cut(s) 15, 157, 717, 803
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 5 cut(s) 598, 670, 1033, 1082, 1153
FaqI GGGAC 2 cut(s) 448, 826
FatI CATG 5 cut(s) 594, 666, 1029, 1078, 1149
FblI GTMKAC 2 cut(s) 611, 941
Fnu4HI GCNGC 4 cut(s) 63, 624, 878, 956
FokI GGATG 3 cut(s) 545, 659, 863
FriOI GRGCYC 4 cut(s) 15, 157, 717, 803
Fsp4HI GCNGC 4 cut(s) 63, 624, 878, 956
FspBI CTAG 3 cut(s) 632, 846, 1178
GlaI GCGC 3 cut(s) 73, 88, 959
GluI GCNGC 4 cut(s) 63, 624, 878, 956
GsuI CTGGAG 1 cut(s) 1067
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 3 cut(s) 26, 65, 191
HapII CCGG 4 cut(s) 78, 240, 432, 438
HgaI GACGC 3 cut(s) 63, 168, 297
HhaI GCGC 3 cut(s) 74, 89, 960
Hin1I GRCGYC 2 cut(s) 87, 179
Hin1II CATG 5 cut(s) 598, 670, 1033, 1082, 1153
Hin6I GCGC 3 cut(s) 72, 87, 958
HinP1I GCGC 3 cut(s) 72, 87, 958
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 463
HinfI GANTC 3 cut(s) 185, 518, 793
HpaII CCGG 4 cut(s) 78, 240, 432, 438
HphI GGTGA 2 cut(s) 994, 1006
Hpy166II GTNNAC 6 cut(s) 100, 324, 612, 896, 942, 1038
Hpy188III TCNNGA 7 cut(s) 19, 121, 515, 698, 977, 1003, 1198
Hpy8I GTNNAC 6 cut(s) 100, 324, 612, 896, 942, 1038
Hpy99I CGWCG 3 cut(s) 152, 251, 313
HpyAV CCTTC 6 cut(s) 16, 217, 409, 697, 824, 877
HpyCH4III ACNGT 5 cut(s) 104, 199, 751, 1009, 1066
HpyCH4V TGCA 8 cut(s) 31, 316, 470, 594, 598, 732, 1105, 1165
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 176, 623
HpyF3I CTNAG 1 cut(s) 1206
Hsp92I GRCGYC 2 cut(s) 87, 179
Hsp92II CATG 5 cut(s) 598, 670, 1033, 1082, 1153
HspAI GCGC 3 cut(s) 72, 87, 958
KasI GGCGCC 1 cut(s) 86
Kzo9I GATC 4 cut(s) 339, 386, 565, 1013
LmnI GCTCC 4 cut(s) 152, 577, 712, 842
Lsp1109I GCAGC 1 cut(s) 889
LweI GCATC 3 cut(s) 40, 541, 703
MaeI CTAG 3 cut(s) 632, 846, 1178
MaeIII GTNAC 5 cut(s) 16, 80, 982, 994, 1144
MalI GATC 4 cut(s) 341, 388, 567, 1015
MboI GATC 4 cut(s) 339, 386, 565, 1013
MboII GAAGA 3 cut(s) 48, 519, 1125
MflI RGATCY 1 cut(s) 1013
MhlI GDGCHC 4 cut(s) 15, 157, 717, 803
MluCI AATT 5 cut(s) 159, 589, 652, 805, 1025
Mly113I GGCGCC 1 cut(s) 87
MmeI TCCRAC 7 cut(s) 130, 237, 270, 273, 364, 378, 439
MroXI GAANNNNTTC 2 cut(s) 419, 934
MslI CAYNNNNRTG 1 cut(s) 1170
MspI CCGG 4 cut(s) 78, 240, 432, 438
MspR9I CCNGG 2 cut(s) 433, 439
Mva1269I GAATGC 1 cut(s) 505
MvnI CGCG 3 cut(s) 62, 74, 958
MwoI GCNNNNNNNGC 3 cut(s) 71, 176, 623
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 2 cut(s) 433, 439
NdeII GATC 4 cut(s) 339, 386, 565, 1013
NlaIII CATG 5 cut(s) 598, 670, 1033, 1082, 1153
NlaIV GGNNCC 4 cut(s) 88, 154, 244, 436
NmeAIII GCCGAG 3 cut(s) 48, 217, 377
NmuCI GTSAC 3 cut(s) 16, 982, 994
NspI RCATGY 2 cut(s) 598, 670
NspV TTCGAA 1 cut(s) 791
PaeI GCATGC 1 cut(s) 598
PceI AGGCCT 1 cut(s) 26
PciI ACATGT 1 cut(s) 666
PctI GAATGC 1 cut(s) 505
PdmI GAANNNNTTC 2 cut(s) 419, 934
PfeI GAWTC 3 cut(s) 185, 518, 793
PflFI GACNNNGTC 1 cut(s) 817
PkrI GCNGC 4 cut(s) 64, 625, 879, 957
Ple19I CGATCG 1 cut(s) 342
PluTI GGCGCC 1 cut(s) 90
PscI ACATGT 1 cut(s) 666
Psp124BI GAGCTC 2 cut(s) 717, 803
PspN4I GGNNCC 4 cut(s) 88, 154, 244, 436
PspPI GGNCC 3 cut(s) 64, 118, 435
PsuI RGATCY 1 cut(s) 1013
PsyI GACNNNGTC 1 cut(s) 817
PvuI CGATCG 1 cut(s) 342
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
RseI CAYNNNNRTG 1 cut(s) 1170
SacI GAGCTC 2 cut(s) 717, 803
SatI GCNGC 4 cut(s) 63, 624, 878, 956
Sau3AI GATC 4 cut(s) 339, 386, 565, 1013
Sau96I GGNCC 3 cut(s) 64, 118, 435
ScrFI CCNGG 2 cut(s) 433, 439
SduI GDGCHC 4 cut(s) 15, 157, 717, 803
SfaNI GCATC 3 cut(s) 40, 541, 703
SfcI CTRYAG 1 cut(s) 91
SfoI GGCGCC 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 791
SinI GGWCC 2 cut(s) 118, 435
SmiMI CAYNNNNRTG 1 cut(s) 1170
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SphI GCATGC 1 cut(s) 598
Sse9I AATT 5 cut(s) 159, 589, 652, 805, 1025
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 6 cut(s) 62, 170, 554, 617, 623, 956
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 3 cut(s) 632, 846, 1178
SstI GAGCTC 2 cut(s) 717, 803
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 431, 437
TaaI ACNGT 5 cut(s) 104, 199, 751, 1009, 1066
TaqI TCGA 5 cut(s) 34, 521, 549, 791, 1002
TasI AATT 5 cut(s) 159, 589, 652, 805, 1025
TauI GCSGC 3 cut(s) 65, 626, 958
TfiI GAWTC 3 cut(s) 185, 518, 793
TseFI GTSAC 3 cut(s) 16, 982, 994
TseI GCWGC 1 cut(s) 877
Tsp45I GTSAC 3 cut(s) 16, 982, 994
TspDTI ATGAA 3 cut(s) 127, 1018, 1067
TspGWI ACGGA 1 cut(s) 341
Tth111I GACNNNGTC 1 cut(s) 817
VpaK11BI GGWCC 2 cut(s) 118, 435
XapI RAATTY 2 cut(s) 805, 1025
XceI RCATGY 2 cut(s) 598, 670
XmiI GTMKAC 2 cut(s) 611, 941
XmnI GAANNNNTTC 2 cut(s) 419, 934
XspI CTAG 3 cut(s) 632, 846, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.