Rw6G042620

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012893
Physical Location & Seq
Forward (+)
66021632 .. 66023258
1627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G042620.1

Sequence Viewer

Length: 1221 bp
ATGGAACTCGGGCTCGTCACGAAGGCCTTCCATCGAAGAAAGAAATGGCTTCTGATACTCGCGGCTCTCGGCGCGTCCGGTTACGGCGCCTACAGGGTTTGCAGTTCGCCCTCTTTGGTCCAGAAGCGAAAGAGGTTCACGAAGGTCGTCGGAGCCCTAATTTCTTTGGCGGAGATGGCGTCTGATTCGGCCGAGACCCTCAGCATTGTCTCCAACGACTTGAAGGAGTTTCTGAACTCCGGTTCCGACGAGATGCCCAAAAGTCTGAAGCAGATTTCCAAGATTGCGAGGTCGGAGGAGTTCTCCGCGTCGCTGCAGAGGCTGACGGAGGCTATGACGATCGGGGTATGCCGAGGGTTTGAGCTTGAGACGAGGAATGGGGCTGGATCGGAGAAGGGTTTGGGGAGTTCGAGCTATTCACTTTCAGAGAGAGTTATGGAGAAGGTTTTCTCCGAAGCCGGGACCGGGTTCGTTTCGGTTGTAGTTGGAAGCTTTGCAAGGAATATGGTTATGGGGTTTTACTCAAATGAGCATTCAGGTCAGGATTCGGATGTCAGTAGATGGGTCGATGCGGTTTGTAGTGATCCAAGTAAGGAGCTGATAGCCAATTGCATACAGGTTTTGGTGTCTACTGCGGTTGCGGCGTACCTAGAGAAAACAAAGGACGTCAATTTCTGTGATGACATGTTTGCTGGAATGACCAATTCCAAGCATCAGGAGAAGGTCAGGGAGCTCTTGGTTTCTGTTTGCAATGGTGCTGTGGAGACTGTTGTGAAAACTTCTCATCAAGTTTTGACGAGTTCTGGTTCGAATCCGAGCTCAAATTCGGGGACTTGGTCAGTTGTTGATCATGGAGCTAGGGATGAGTGCCTTGACCAAGAAGCAAAGCAGCCCAAGGAGGGAAGTTCACTTGAGGCTATTCAGAATAGCGAGTGGATTGGAAAGGTTTCGTCTACATTGGCAGTGCCGCGCAATAGGAAGTTTGTTCGTGAGGTGACTGGGAGGGTGACATTCGAGACTGTAAGATCTTTTGTTGAATTCATGTTGTGGGCACTACTAGAGGGGTTGAAAAGAACTGTTAGTGTGGTTCATGGGGAGATTGTGGGTAGGGGGTTGCAAGTCATCAGATATTTTGGCGCAAAGTCTTCGGCTATTGTTACCATATGTCTTGCTTTGCACTTGTATGTTCTAGGAGGTAGTAGAGTTCTTTTGACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

406

Amino Acids

44.08

Weight (kDa)

8.53

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 669
AccB1I GGYRCC 1 cut(s) 86
AccI GTMKAC 2 cut(s) 629, 953
AccII CGCG 4 cut(s) 62, 74, 308, 970
AciI CCGC 7 cut(s) 62, 170, 306, 572, 635, 641, 968
AclWI GGATC 2 cut(s) 394, 578
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 2 cut(s) 823, 1037
AcuI CTGAAG 1 cut(s) 287
AcyI GRCGYC 3 cut(s) 87, 179, 666
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 3 cut(s) 459, 465, 899
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 3 cut(s) 223, 1037, 1069
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 7 cut(s) 364, 414, 492, 598, 733, 819, 857
AluI AGCT 7 cut(s) 364, 414, 492, 598, 733, 819, 857
Alw21I GWGCWC 2 cut(s) 735, 821
Alw26I GTCTC 5 cut(s) 188, 214, 362, 758, 1010
AlwI GGATC 2 cut(s) 394, 578
AlwNI CAGNNNCTG 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 2 cut(s) 24, 189
ApeKI GCWGC 2 cut(s) 313, 889
ApoI RAATTY 2 cut(s) 823, 1037
Asp700I GAANNNNTTC 3 cut(s) 26, 446, 946
AspLEI GCGC 4 cut(s) 74, 89, 972, 1139
AspS9I GGNCC 2 cut(s) 118, 462
AsuC2I CCSGG 2 cut(s) 460, 466
AsuHPI GGTGA 2 cut(s) 1006, 1018
AsuII TTCGAA 1 cut(s) 809
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 118, 462
BaeGI GKGCMC 1 cut(s) 1054
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 4 cut(s) 15, 157, 735, 821
BbsI GAAGAC 1 cut(s) 1137
Bbv12I GWGCWC 2 cut(s) 735, 821
BbvCI CCTCAGC 1 cut(s) 200
BbvI GCAGC 2 cut(s) 300, 901
BccI CCATC 3 cut(s) 39, 169, 555
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 2 cut(s) 1128, 1162
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 2 cut(s) 460, 466
BcoDI GTCTC 5 cut(s) 188, 214, 362, 758, 1010
BfaI CTAG 4 cut(s) 650, 858, 1058, 1190
BfmI CTRYAG 2 cut(s) 91, 314
BfoI RGCGCY 1 cut(s) 90
BglII AGATCT 1 cut(s) 1025
BisI GCNGC 5 cut(s) 63, 314, 642, 890, 968
BlsI GCNGC 5 cut(s) 64, 315, 643, 891, 969
Bme1390I CCNGG 2 cut(s) 460, 466
Bme18I GGWCC 2 cut(s) 118, 462
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 118, 462
BmiI GGNNCC 4 cut(s) 88, 154, 244, 463
BmrFI CCNGG 2 cut(s) 460, 466
BmrI ACTGGG 1 cut(s) 1008
BmsI GCATC 3 cut(s) 243, 559, 721
BmuI ACTGGG 1 cut(s) 1008
BpiI GAAGAC 1 cut(s) 1137
BplI GAGNNNNNCTC 2 cut(s) 287, 319
Bpu10I CCTNAGC 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 809
BpuEI CTTGAG 2 cut(s) 386, 932
BpuMI CCSGG 2 cut(s) 460, 466
BsaHI GRCGYC 3 cut(s) 87, 179, 666
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 352, 894
BsaWI WCCGGW 2 cut(s) 77, 239
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 3 cut(s) 459, 465, 899
Bse1I ACTGG 1 cut(s) 1003
Bse3DI GCAATG 1 cut(s) 757
BseDI CCNNGG 2 cut(s) 352, 894
BseGI GGATG 2 cut(s) 556, 868
BseLI CCNNNNNNNGG 3 cut(s) 459, 465, 899
BseMI GCAATG 1 cut(s) 757
BseMII CTCAG 1 cut(s) 214
BseNI ACTGG 1 cut(s) 1003
BseRI GAGGAG 1 cut(s) 311
BseSI GKGCMC 1 cut(s) 1054
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 2 cut(s) 300, 901
Bsh1236I CGCG 4 cut(s) 62, 74, 308, 970
Bsh1285I CGRYCG 2 cut(s) 192, 342
BshFI GGCC 2 cut(s) 26, 191
BshNI GGYRCC 1 cut(s) 86
BsiEI CGRYCG 2 cut(s) 192, 342
BsiHKAI GWGCWC 2 cut(s) 735, 821
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 4 cut(s) 78, 240, 459, 465
BslFI GGGAC 2 cut(s) 475, 844
BslI CCNNNNNNNGG 3 cut(s) 459, 465, 899
BsmAI GTCTC 5 cut(s) 188, 214, 362, 758, 1010
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 2 cut(s) 475, 844
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 2 cut(s) 26, 191
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 809
Bsp1286I GDGCHC 5 cut(s) 15, 157, 735, 821, 1054
Bsp143I GATC 5 cut(s) 339, 386, 583, 847, 1025
BspACI CCGC 7 cut(s) 62, 170, 306, 572, 635, 641, 968
BspANI GGCC 2 cut(s) 26, 191
BspCNI CTCAG 1 cut(s) 213
BspFNI CGCG 4 cut(s) 62, 74, 308, 970
BspLI GGNNCC 4 cut(s) 88, 154, 244, 463
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 2 cut(s) 394, 578
BspT104I TTCGAA 1 cut(s) 809
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 757
BsrI ACTGG 1 cut(s) 1003
BssECI CCNNGG 2 cut(s) 352, 894
BssMI GATC 5 cut(s) 339, 386, 583, 847, 1025
BssNI GRCGYC 3 cut(s) 87, 179, 666
BssT1I CCWWGG 1 cut(s) 894
Bst4CI ACNGT 3 cut(s) 769, 1021, 1078
BstACI GRCGYC 3 cut(s) 87, 179, 666
BstBI TTCGAA 1 cut(s) 809
BstDEI CTNAG 2 cut(s) 200, 1218
BstF5I GGATG 2 cut(s) 556, 868
BstFNI CGCG 4 cut(s) 62, 74, 308, 970
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 4 cut(s) 74, 89, 972, 1139
BstKTI GATC 5 cut(s) 342, 389, 586, 850, 1028
BstMAI GTCTC 5 cut(s) 188, 214, 362, 758, 1010
BstMBI GATC 5 cut(s) 339, 386, 583, 847, 1025
BstMCI CGRYCG 2 cut(s) 192, 342
BstMWI GCNNNNNNNGC 4 cut(s) 71, 176, 319, 641
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 2 cut(s) 458, 464
BstSFI CTRYAG 2 cut(s) 91, 314
BstSLI GKGCMC 1 cut(s) 1054
BstUI CGCG 4 cut(s) 62, 74, 308, 970
BstV1I GCAGC 2 cut(s) 300, 901
BstV2I GAAGAC 1 cut(s) 1137
BstX2I RGATCY 1 cut(s) 1025
BstYI RGATCY 1 cut(s) 1025
BstZI CGGCCG 1 cut(s) 189
BsuRI GGCC 2 cut(s) 26, 191
BtsCI GGATG 2 cut(s) 556, 868
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 1 cut(s) 969
CaiI CAGNNNCTG 1 cut(s) 322
CfoI GCGC 4 cut(s) 74, 89, 972, 1139
Cfr13I GGNCC 2 cut(s) 118, 462
CseI GACGC 3 cut(s) 63, 168, 297
Csp6I GTAC 1 cut(s) 646
CviAII CATG 4 cut(s) 685, 851, 1042, 1091
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 2 cut(s) 200, 1218
DinI GGCGCC 1 cut(s) 88
DpnI GATC 5 cut(s) 341, 388, 585, 849, 1027
DpnII GATC 5 cut(s) 339, 386, 583, 847, 1025
EaeI YGGCCR 1 cut(s) 189
EagI CGGCCG 1 cut(s) 189
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 2 cut(s) 733, 819
EclXI CGGCCG 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 894
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 4 cut(s) 15, 157, 735, 821
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 118, 462
Eco52I CGGCCG 1 cut(s) 189
Eco53kI GAGCTC 2 cut(s) 733, 819
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 733, 819
EcoRI GAATTC 1 cut(s) 1037
EcoT14I CCWWGG 1 cut(s) 894
EcoT38I GRGCYC 4 cut(s) 15, 157, 735, 821
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 894
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 4 cut(s) 688, 854, 1045, 1094
FaqI GGGAC 2 cut(s) 475, 844
FatI CATG 4 cut(s) 684, 850, 1041, 1090
FauNDI CATATG 1 cut(s) 1163
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 2 cut(s) 629, 953
Fnu4HI GCNGC 5 cut(s) 63, 314, 642, 890, 968
FokI GGATG 2 cut(s) 563, 875
FriOI GRGCYC 4 cut(s) 15, 157, 735, 821
Fsp4HI GCNGC 5 cut(s) 63, 314, 642, 890, 968
FspBI CTAG 4 cut(s) 650, 858, 1058, 1190
GlaI GCGC 4 cut(s) 73, 88, 971, 1138
GluI GCNGC 5 cut(s) 63, 314, 642, 890, 968
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 2 cut(s) 26, 191
HapII CCGG 4 cut(s) 78, 240, 459, 465
HgaI GACGC 3 cut(s) 63, 168, 297
HhaI GCGC 4 cut(s) 74, 89, 972, 1139
Hin1I GRCGYC 3 cut(s) 87, 179, 666
Hin1II CATG 4 cut(s) 688, 854, 1045, 1094
Hin6I GCGC 4 cut(s) 72, 87, 970, 1137
HinP1I GCGC 4 cut(s) 72, 87, 970, 1137
HindIII AAGCTT 1 cut(s) 490
HinfI GANTC 3 cut(s) 185, 545, 811
HpaII CCGG 4 cut(s) 78, 240, 459, 465
HphI GGTGA 2 cut(s) 1006, 1018
Hpy166II GTNNAC 4 cut(s) 138, 630, 908, 954
Hpy188III TCNNGA 7 cut(s) 19, 121, 139, 542, 716, 989, 1015
Hpy8I GTNNAC 4 cut(s) 138, 630, 908, 954
Hpy99I CGWCG 3 cut(s) 152, 251, 313
HpyAV CCTTC 7 cut(s) 16, 37, 136, 217, 388, 436, 715
HpyCH4III ACNGT 3 cut(s) 769, 1021, 1078
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 7 cut(s) 102, 316, 497, 612, 750, 1117, 1177
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 176, 319, 641
HpyF3I CTNAG 2 cut(s) 200, 1218
HpySE526I ACGT 1 cut(s) 666
Hsp92I GRCGYC 3 cut(s) 87, 179, 666
Hsp92II CATG 4 cut(s) 688, 854, 1045, 1094
HspAI GCGC 4 cut(s) 72, 87, 970, 1137
KasI GGCGCC 1 cut(s) 86
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 5 cut(s) 339, 386, 583, 847, 1025
LmnI GCTCC 4 cut(s) 152, 595, 730, 854
Lsp1109I GCAGC 2 cut(s) 300, 901
LweI GCATC 3 cut(s) 243, 559, 721
MaeI CTAG 4 cut(s) 650, 858, 1058, 1190
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 5 cut(s) 16, 80, 994, 1006, 1156
MalI GATC 5 cut(s) 341, 388, 585, 849, 1027
MboI GATC 5 cut(s) 339, 386, 583, 847, 1025
MboII GAAGA 2 cut(s) 48, 1137
MfeI CAATTG 1 cut(s) 607
MflI RGATCY 1 cut(s) 1025
MhlI GDGCHC 5 cut(s) 15, 157, 735, 821, 1054
MluCI AATT 6 cut(s) 159, 607, 670, 703, 823, 1037
Mly113I GGCGCC 1 cut(s) 87
MmeI TCCRAC 5 cut(s) 130, 237, 270, 273, 466
MroXI GAANNNNTTC 3 cut(s) 26, 446, 946
MslI CAYNNNNRTG 1 cut(s) 1182
MspI CCGG 4 cut(s) 78, 240, 459, 465
MspR9I CCNGG 2 cut(s) 460, 466
MunI CAATTG 1 cut(s) 607
Mva1269I GAATGC 1 cut(s) 532
MvnI CGCG 4 cut(s) 62, 74, 308, 970
MwoI GCNNNNNNNGC 4 cut(s) 71, 176, 319, 641
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 2 cut(s) 460, 466
NdeI CATATG 1 cut(s) 1163
NdeII GATC 5 cut(s) 339, 386, 583, 847, 1025
NlaIII CATG 4 cut(s) 688, 854, 1045, 1094
NlaIV GGNNCC 4 cut(s) 88, 154, 244, 463
NmeAIII GCCGAG 3 cut(s) 48, 217, 377
NmuCI GTSAC 3 cut(s) 16, 994, 1006
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 809
PceI AGGCCT 1 cut(s) 26
PciI ACATGT 1 cut(s) 684
PctI GAATGC 1 cut(s) 532
PdmI GAANNNNTTC 3 cut(s) 26, 446, 946
PfeI GAWTC 3 cut(s) 185, 545, 811
PflFI GACNNNGTC 1 cut(s) 835
PkrI GCNGC 5 cut(s) 64, 315, 643, 891, 969
Ple19I CGATCG 1 cut(s) 342
PluTI GGCGCC 1 cut(s) 90
PscI ACATGT 1 cut(s) 684
Psp124BI GAGCTC 2 cut(s) 735, 821
PspN4I GGNNCC 4 cut(s) 88, 154, 244, 463
PspPI GGNCC 2 cut(s) 118, 462
PstI CTGCAG 1 cut(s) 318
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 1025
PsyI GACNNNGTC 1 cut(s) 835
PvuI CGATCG 1 cut(s) 342
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 1 cut(s) 1182
SacI GAGCTC 2 cut(s) 735, 821
SatI GCNGC 5 cut(s) 63, 314, 642, 890, 968
Sau3AI GATC 5 cut(s) 339, 386, 583, 847, 1025
Sau96I GGNCC 2 cut(s) 118, 462
ScrFI CCNGG 2 cut(s) 460, 466
SduI GDGCHC 5 cut(s) 15, 157, 735, 821, 1054
SfaNI GCATC 3 cut(s) 243, 559, 721
SfcI CTRYAG 2 cut(s) 91, 314
SfoI GGCGCC 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 809
SinI GGWCC 2 cut(s) 118, 462
SmiMI CAYNNNNRTG 1 cut(s) 1182
SmlI CTYRAG 2 cut(s) 365, 911
SmoI CTYRAG 2 cut(s) 365, 911
Sse9I AATT 6 cut(s) 159, 607, 670, 703, 823, 1037
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 7 cut(s) 62, 170, 306, 572, 635, 641, 968
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 4 cut(s) 650, 858, 1058, 1190
SstI GAGCTC 2 cut(s) 735, 821
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 458, 464
StyI CCWWGG 1 cut(s) 894
TaaI ACNGT 3 cut(s) 769, 1021, 1078
TaiI ACGT 1 cut(s) 669
TaqI TCGA 5 cut(s) 34, 410, 567, 809, 1014
TasI AATT 6 cut(s) 159, 607, 670, 703, 823, 1037
TauI GCSGC 3 cut(s) 65, 644, 970
TfiI GAWTC 3 cut(s) 185, 545, 811
TscAI CASTG 1 cut(s) 969
TseFI GTSAC 3 cut(s) 16, 994, 1006
TseI GCWGC 2 cut(s) 313, 889
Tsp45I GTSAC 3 cut(s) 16, 994, 1006
TspDTI ATGAA 2 cut(s) 1030, 1079
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 969
Tth111I GACNNNGTC 1 cut(s) 835
VpaK11BI GGWCC 2 cut(s) 118, 462
XapI RAATTY 2 cut(s) 823, 1037
XceI RCATGY 1 cut(s) 688
XmiI GTMKAC 2 cut(s) 629, 953
XmnI GAANNNNTTC 3 cut(s) 26, 446, 946
XspI CTAG 4 cut(s) 650, 858, 1058, 1190
ZraI GACGTC 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.