Rh6AG489700

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012893
Physical Location & Seq
Forward (+)
66654228 .. 66656341
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG489700.1

Sequence Viewer

Length: 1209 bp
ATGGAACTCGGGCTCGTGACGAAGGCCTTGCATCGAAGAAAGAAATGGCTTCTGATACTCGCGGCTCTCGGCGCGTCCGGTTACGGCGCCTACAGGGTTTACAATTCGCCCTCTTTGGTCCAGAAGCGAAAGAGGTTCATGAAGGTCGTCGGAGCCCTAATTTCTACGGCGGAGATGGCGTCTGATTCAGCCGAGGCCCTCAGCATTGTCTCCAACGACTTGAAGGAGTTTCTGAACTCCGGTTCCGACGAAATGCCCAAAAGTCTGAAGCAGATTTCCAAGATTGCGAGGTCGGAGGAGTTCTCCGCGTCGCTGCAGAGGTTGACGGAGGCTATGACGATCGGGGTATGCCGAGGGTTTGAGTTTGAGACGAGGAATGGGGTTGGATCGGAGAATTCGAGCTATTCACTTTCAGAGAGAGTTATGGAGAAGGTTTTCTCTGAAGCCGGGACCGGGTTCGTTTCGGTTGTAGTTGGAAGCTTTGCAAGGAATATGGTTATGGGGTTTTACTCAAATGAGCATTCAGGTCAGGATTCGGATGTCAATAGATGGGTCGATGCGGTTTGTAGTGATCCAAGTAAGGAGCTGATAGGCAATTGCATACAGGTTTTGGTGTCTACTGCGGTTGCGGCGTACCTAGACAAAACGAAGGATGTCAATTTCTGTGATGACATGTTTGGTGGAATGACCAATTCCAAGCATCAGGAGAAGGTCAGGGAGCTCTTGGTCTCTGTTTGCAATGGTGCTGTGGAGACTGTTGTGAAAACTTCTCATCAAGTTTTGACGACTTCTGGTTCGAATCCGAGCTCGAATTCGGGGACTTGGTCAGCTGTTGATCATGGAGCTAGGGATGAGTGCCTTGAGCAAGAAGCAAAGCAGCCCAAGGAGGGAAGTTCACTTGAGGCTATTCAGAATAGCGAGTGGATTGGAAAGGTTTCGTCTACATTGGCAGTGCCGCGCAATAGGAAGTTTGTTCGTGAGGTGACTGGGAGGGTGACATTCGAGACTGTAAGATCTTTTGTTGAATTCATGTTGTGGACAATACTAGAGGGGTTGAAAAGAACTCTTAGTGTGGTTCATGGGGAGATTGTGGGTAGGGGGTTGCAAGTTATCAGATATTTTGGTGCAAAGTCTTCGGCTATTGTTACCATATGTCTTGCTTTGCACTTGTATGTTCTAGGAGGTAGTAGAGTTCTCTTGACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

43.89

Weight (kDa)

8.38

Isoelectric Point (pI)

28.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccI GTMKAC 2 cut(s) 617, 941
AccII CGCG 4 cut(s) 62, 74, 308, 958
AciI CCGC 7 cut(s) 62, 170, 306, 560, 623, 629, 956
AclWI GGATC 2 cut(s) 394, 566
AcsI RAATTY 3 cut(s) 394, 811, 1025
AcuI CTGAAG 2 cut(s) 287, 462
AcyI GRCGYC 2 cut(s) 87, 179
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 2 cut(s) 453, 887
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 3 cut(s) 223, 1025, 1057
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 7 cut(s) 402, 480, 586, 721, 807, 830, 845
AluI AGCT 7 cut(s) 402, 480, 586, 721, 807, 830, 845
Alw21I GWGCWC 2 cut(s) 723, 809
Alw26I GTCTC 5 cut(s) 214, 362, 733, 746, 998
AlwI GGATC 2 cut(s) 394, 566
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 2 cut(s) 24, 195
ApeKI GCWGC 2 cut(s) 313, 877
ApoI RAATTY 3 cut(s) 394, 811, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 778, 810
Asp700I GAANNNNTTC 2 cut(s) 434, 934
AspLEI GCGC 3 cut(s) 74, 89, 960
AspS9I GGNCC 3 cut(s) 118, 196, 450
AsuC2I CCSGG 2 cut(s) 448, 454
AsuHPI GGTGA 2 cut(s) 994, 1006
AsuII TTCGAA 1 cut(s) 797
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 118, 450
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 4 cut(s) 15, 157, 723, 809
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 1125
Bbv12I GWGCWC 2 cut(s) 723, 809
BbvCI CCTCAGC 1 cut(s) 200
BbvI GCAGC 2 cut(s) 300, 889
BccI CCATC 2 cut(s) 169, 543
BceAI ACGGC 2 cut(s) 100, 183
BcgI CGANNNNNNTGC 4 cut(s) 10, 44, 1116, 1150
BclI TGATCA 1 cut(s) 835
BcnI CCSGG 2 cut(s) 448, 454
BcoDI GTCTC 5 cut(s) 214, 362, 733, 746, 998
BfaI CTAG 4 cut(s) 638, 846, 1046, 1178
BfmI CTRYAG 2 cut(s) 91, 314
BfoI RGCGCY 1 cut(s) 90
BglII AGATCT 1 cut(s) 1013
BisI GCNGC 5 cut(s) 63, 314, 630, 878, 956
BlsI GCNGC 5 cut(s) 64, 315, 631, 879, 957
Bme1390I CCNGG 2 cut(s) 448, 454
Bme18I GGWCC 2 cut(s) 118, 450
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 3 cut(s) 118, 196, 450
BmiI GGNNCC 4 cut(s) 88, 154, 244, 451
BmrFI CCNGG 2 cut(s) 448, 454
BmrI ACTGGG 1 cut(s) 996
BmsI GCATC 3 cut(s) 40, 547, 709
BmuI ACTGGG 1 cut(s) 996
BpiI GAAGAC 1 cut(s) 1125
BplI GAGNNNNNCTC 2 cut(s) 287, 319
Bpu10I CCTNAGC 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 797
BpuEI CTTGAG 2 cut(s) 881, 920
BpuMI CCSGG 2 cut(s) 448, 454
BsaHI GRCGYC 2 cut(s) 87, 179
BsaI GGTCTC 1 cut(s) 733
BsaJI CCNNGG 3 cut(s) 192, 352, 882
BsaWI WCCGGW 2 cut(s) 77, 239
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 887
Bse1I ACTGG 1 cut(s) 991
Bse3DI GCAATG 1 cut(s) 745
BseDI CCNNGG 3 cut(s) 192, 352, 882
BseGI GGATG 3 cut(s) 544, 658, 856
BseLI CCNNNNNNNGG 2 cut(s) 453, 887
BseMI GCAATG 1 cut(s) 745
BseMII CTCAG 1 cut(s) 214
BseNI ACTGG 1 cut(s) 991
BseRI GAGGAG 1 cut(s) 311
BseXI GCAGC 2 cut(s) 300, 889
Bsh1236I CGCG 4 cut(s) 62, 74, 308, 958
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 2 cut(s) 26, 197
BshNI GGYRCC 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 2 cut(s) 723, 809
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 4 cut(s) 78, 240, 447, 453
BslFI GGGAC 2 cut(s) 463, 832
BslI CCNNNNNNNGG 2 cut(s) 453, 887
BsmAI GTCTC 5 cut(s) 214, 362, 733, 746, 998
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 2 cut(s) 463, 832
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 2 cut(s) 26, 197
Bso31I GGTCTC 1 cut(s) 733
BsoBI CYCGRG 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 797
Bsp1286I GDGCHC 4 cut(s) 15, 157, 723, 809
Bsp143I GATC 5 cut(s) 339, 386, 571, 835, 1013
BspACI CCGC 7 cut(s) 62, 170, 306, 560, 623, 629, 956
BspANI GGCC 2 cut(s) 26, 197
BspCNI CTCAG 1 cut(s) 213
BspFNI CGCG 4 cut(s) 62, 74, 308, 958
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 4 cut(s) 88, 154, 244, 451
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 2 cut(s) 394, 566
BspT104I TTCGAA 1 cut(s) 797
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 733
BsrDI GCAATG 1 cut(s) 745
BsrI ACTGG 1 cut(s) 991
BssECI CCNNGG 3 cut(s) 192, 352, 882
BssMI GATC 5 cut(s) 339, 386, 571, 835, 1013
BssNI GRCGYC 2 cut(s) 87, 179
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 882
Bst2BI CACGAG 1 cut(s) 14
Bst4CI ACNGT 2 cut(s) 757, 1009
BstACI GRCGYC 2 cut(s) 87, 179
BstBI TTCGAA 1 cut(s) 797
BstDEI CTNAG 3 cut(s) 200, 1067, 1206
BstF5I GGATG 3 cut(s) 544, 658, 856
BstFNI CGCG 4 cut(s) 62, 74, 308, 958
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 74, 89, 960
BstKTI GATC 5 cut(s) 342, 389, 574, 838, 1016
BstMAI GTCTC 5 cut(s) 214, 362, 733, 746, 998
BstMBI GATC 5 cut(s) 339, 386, 571, 835, 1013
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 3 cut(s) 71, 176, 629
BstNSI RCATGY 1 cut(s) 676
BstSCI CCNGG 2 cut(s) 446, 452
BstSFI CTRYAG 2 cut(s) 91, 314
BstUI CGCG 4 cut(s) 62, 74, 308, 958
BstV1I GCAGC 2 cut(s) 300, 889
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 1013
BsuRI GGCC 2 cut(s) 26, 197
BtsCI GGATG 3 cut(s) 544, 658, 856
BtsI GCAGTG 1 cut(s) 957
BtsIMutI CAGTG 1 cut(s) 957
CciI TCATGA 1 cut(s) 138
CfoI GCGC 3 cut(s) 74, 89, 960
Cfr13I GGNCC 3 cut(s) 118, 196, 450
CseI GACGC 3 cut(s) 63, 168, 297
Csp6I GTAC 1 cut(s) 634
CviAII CATG 5 cut(s) 139, 673, 839, 1030, 1079
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 3 cut(s) 200, 1067, 1206
DinI GGCGCC 1 cut(s) 88
DpnI GATC 5 cut(s) 341, 388, 573, 837, 1015
DpnII GATC 5 cut(s) 339, 386, 571, 835, 1013
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 2 cut(s) 721, 807
Eco130I CCWWGG 1 cut(s) 882
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 4 cut(s) 15, 157, 723, 809
Eco31I GGTCTC 1 cut(s) 733
Eco47I GGWCC 2 cut(s) 118, 450
Eco53kI GAGCTC 2 cut(s) 721, 807
Eco57I CTGAAG 2 cut(s) 287, 462
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 721, 807
EcoO109I RGGNCCY 1 cut(s) 196
EcoRI GAATTC 3 cut(s) 394, 811, 1025
EcoT14I CCWWGG 1 cut(s) 882
EcoT38I GRGCYC 4 cut(s) 15, 157, 723, 809
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 882
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 5 cut(s) 142, 676, 842, 1033, 1082
FaqI GGGAC 2 cut(s) 463, 832
FatI CATG 5 cut(s) 138, 672, 838, 1029, 1078
FauNDI CATATG 1 cut(s) 1151
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 2 cut(s) 617, 941
Fnu4HI GCNGC 5 cut(s) 63, 314, 630, 878, 956
FokI GGATG 3 cut(s) 551, 665, 863
FriOI GRGCYC 4 cut(s) 15, 157, 723, 809
Fsp4HI GCNGC 5 cut(s) 63, 314, 630, 878, 956
FspBI CTAG 4 cut(s) 638, 846, 1046, 1178
GlaI GCGC 3 cut(s) 73, 88, 959
GluI GCNGC 5 cut(s) 63, 314, 630, 878, 956
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 2 cut(s) 26, 197
HapII CCGG 4 cut(s) 78, 240, 447, 453
HgaI GACGC 3 cut(s) 63, 168, 297
HhaI GCGC 3 cut(s) 74, 89, 960
Hin1I GRCGYC 2 cut(s) 87, 179
Hin1II CATG 5 cut(s) 142, 676, 842, 1033, 1082
Hin6I GCGC 3 cut(s) 72, 87, 958
HinP1I GCGC 3 cut(s) 72, 87, 958
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 478
HinfI GANTC 3 cut(s) 185, 533, 799
HpaII CCGG 4 cut(s) 78, 240, 447, 453
HphI GGTGA 2 cut(s) 994, 1006
Hpy166II GTNNAC 6 cut(s) 100, 324, 618, 896, 942, 1038
Hpy188III TCNNGA 8 cut(s) 16, 121, 139, 530, 704, 977, 1003, 1198
Hpy8I GTNNAC 6 cut(s) 100, 324, 618, 896, 942, 1038
Hpy99I CGWCG 3 cut(s) 152, 251, 313
HpyAV CCTTC 6 cut(s) 16, 136, 217, 424, 643, 703
HpyCH4III ACNGT 2 cut(s) 757, 1009
HpyCH4V TGCA 8 cut(s) 31, 316, 485, 600, 738, 1105, 1127, 1165
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 176, 629
HpyF3I CTNAG 3 cut(s) 200, 1067, 1206
Hsp92I GRCGYC 2 cut(s) 87, 179
Hsp92II CATG 5 cut(s) 142, 676, 842, 1033, 1082
HspAI GCGC 3 cut(s) 72, 87, 958
KasI GGCGCC 1 cut(s) 86
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 5 cut(s) 339, 386, 571, 835, 1013
LmnI GCTCC 4 cut(s) 152, 583, 718, 842
Lsp1109I GCAGC 2 cut(s) 300, 889
LweI GCATC 3 cut(s) 40, 547, 709
MaeI CTAG 4 cut(s) 638, 846, 1046, 1178
MaeIII GTNAC 5 cut(s) 16, 80, 982, 994, 1144
MalI GATC 5 cut(s) 341, 388, 573, 837, 1015
MboI GATC 5 cut(s) 339, 386, 571, 835, 1013
MboII GAAGA 2 cut(s) 48, 1125
MfeI CAATTG 1 cut(s) 595
MflI RGATCY 1 cut(s) 1013
MhlI GDGCHC 4 cut(s) 15, 157, 723, 809
MluCI AATT 8 cut(s) 103, 159, 394, 595, 658, 691, 811, 1025
Mly113I GGCGCC 1 cut(s) 87
MmeI TCCRAC 6 cut(s) 130, 237, 270, 273, 364, 454
MroXI GAANNNNTTC 2 cut(s) 434, 934
MslI CAYNNNNRTG 1 cut(s) 1170
MspA1I CMGCKG 1 cut(s) 830
MspI CCGG 4 cut(s) 78, 240, 447, 453
MspR9I CCNGG 2 cut(s) 448, 454
MunI CAATTG 1 cut(s) 595
Mva1269I GAATGC 1 cut(s) 520
MvnI CGCG 4 cut(s) 62, 74, 308, 958
MwoI GCNNNNNNNGC 3 cut(s) 71, 176, 629
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 2 cut(s) 448, 454
NdeI CATATG 1 cut(s) 1151
NdeII GATC 5 cut(s) 339, 386, 571, 835, 1013
NlaIII CATG 5 cut(s) 142, 676, 842, 1033, 1082
NlaIV GGNNCC 4 cut(s) 88, 154, 244, 451
NmeAIII GCCGAG 3 cut(s) 48, 217, 377
NmuCI GTSAC 3 cut(s) 16, 982, 994
NspI RCATGY 1 cut(s) 676
NspV TTCGAA 1 cut(s) 797
PagI TCATGA 1 cut(s) 138
PceI AGGCCT 1 cut(s) 26
PciI ACATGT 1 cut(s) 672
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PctI GAATGC 1 cut(s) 520
PdmI GAANNNNTTC 2 cut(s) 434, 934
PfeI GAWTC 3 cut(s) 185, 533, 799
PflFI GACNNNGTC 1 cut(s) 823
PkrI GCNGC 5 cut(s) 64, 315, 631, 879, 957
Ple19I CGATCG 1 cut(s) 342
PluTI GGCGCC 1 cut(s) 90
PscI ACATGT 1 cut(s) 672
Psp124BI GAGCTC 2 cut(s) 723, 809
PspN4I GGNNCC 4 cut(s) 88, 154, 244, 451
PspPI GGNCC 3 cut(s) 118, 196, 450
PstI CTGCAG 1 cut(s) 318
PsuI RGATCY 1 cut(s) 1013
PsyI GACNNNGTC 1 cut(s) 823
PvuI CGATCG 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 830
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 1170
SacI GAGCTC 2 cut(s) 723, 809
SatI GCNGC 5 cut(s) 63, 314, 630, 878, 956
Sau3AI GATC 5 cut(s) 339, 386, 571, 835, 1013
Sau96I GGNCC 3 cut(s) 118, 196, 450
ScrFI CCNGG 2 cut(s) 448, 454
SduI GDGCHC 4 cut(s) 15, 157, 723, 809
SfaNI GCATC 3 cut(s) 40, 547, 709
SfcI CTRYAG 2 cut(s) 91, 314
SfoI GGCGCC 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 797
SinI GGWCC 2 cut(s) 118, 450
SmiMI CAYNNNNRTG 1 cut(s) 1170
SmlI CTYRAG 2 cut(s) 860, 899
SmoI CTYRAG 2 cut(s) 860, 899
Sse9I AATT 8 cut(s) 103, 159, 394, 595, 658, 691, 811, 1025
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 7 cut(s) 62, 170, 306, 560, 623, 629, 956
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 4 cut(s) 638, 846, 1046, 1178
SstI GAGCTC 2 cut(s) 723, 809
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 446, 452
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 2 cut(s) 757, 1009
TaqI TCGA 6 cut(s) 34, 398, 555, 797, 809, 1002
TasI AATT 8 cut(s) 103, 159, 394, 595, 658, 691, 811, 1025
TauI GCSGC 3 cut(s) 65, 632, 958
TfiI GAWTC 3 cut(s) 185, 533, 799
TscAI CASTG 1 cut(s) 957
TseFI GTSAC 3 cut(s) 16, 982, 994
TseI GCWGC 2 cut(s) 313, 877
Tsp45I GTSAC 3 cut(s) 16, 982, 994
TspDTI ATGAA 4 cut(s) 127, 155, 1018, 1067
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 957
Tth111I GACNNNGTC 1 cut(s) 823
VpaK11BI GGWCC 2 cut(s) 118, 450
XapI RAATTY 3 cut(s) 394, 811, 1025
XceI RCATGY 1 cut(s) 676
XmiI GTMKAC 2 cut(s) 617, 941
XmnI GAANNNNTTC 2 cut(s) 434, 934
XspI CTAG 4 cut(s) 638, 846, 1046, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.