MD08G1079200.v1.1

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012893
Physical Location & Seq
Reverse (-)
6594270 .. 6595538
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1079200.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGGAACTTGGGCTCGTCGCTAAGGGCTTGAATTTCTCTCGGAGAAGAAGGCGATGGCTTCTGTTACTCGCAGCACTTGGGGTCTCCGGCTATGGAGCTTATAGGGTTTACAATTTGCCCTCTGTGGCCAGGAAGAGGAAGAGGTTCCTGAAGCTCGTAGGAGCCCTAATTTCTATGGTCGAGATGGTCTCCGATTCGGCCGAGACGATTGGTGTTGTCTGTGGGGATTTGAAGGAGTTTCTGCGTTCTGATTCGGACGAAATGCCCCAAAGTTTAAAGCAGATTTCAAAAATTGCGAGGTCGGAGGAGTTCTCTGTGTCACTGAGTAGGATTACCGAGGCTATGACGGTTGGGATGATTCGAGGGTGTAAATTGGAGATGAAGAATGAGAATGAATCGGGGAAGGGTTTGGAGAATTCGAGCTTTTCGGATAGGGTTTTCGAGAAAGTTTTCTCTACGGCTGGAACCGGGTTCGTTTCGGTTGTGGTTGGAAGCTTTGCTAAGAATTTGGTTATGGGATTTTACTCTGATGAGCAATCGGGTGAGAATTCGAGTGTGTCGAAATCAAGTCCATCAGATGTGCCTAGATGGGTTAATGTTGTTTGCAGTGATAGAAGCAAGGAGCTCATAGCAAATTGCATTCAGGTTTTCGTAAGCACTGCGGTTGCGGTTTACCTTGACAAAACGAAAGATATCAACGTTTATGAAGAAATGTTCGATGGGTTGACCAATCCCAAACATCAGAACAGAGTGAGGGATGTTCTGGTTTCTGTTTGCAATGGTGCTGTGGAAACAGTTGTGAGGACATCTCACCAAGTTTTGACAAGTTCCAGTTCGAGTTCAAGCTCGAATTCGCATGTGTGTTCAATTGTTGATCAGAGTGAAGGTCCAAGTGGAACTATGGATGAGTGCTTGCATCGAGAGGTATCTCCAAAGCAGCTGAAGGAGGGAAGTTCGTTTGATGATATTCAGAATAGTGGATGGGTTAGGAAGGTTTCATCTACATTGGCAGTTCCGTGCAATAGGAGGTTTGTTTTTGATGTGACGGGGAGGGTGACGTTCGAGACAGTTCGATCTCTCGTTGAGTTTATGTTGTGGATGCTATTAGATGGTTTGAAAAGAAGCCTTAGTGCACTTCATGGGGAGGTTGCAGACAGGGGATTGCAAGTCATCACATACTTCAGTGGAAAGTCTTCGGTTATAGTTACTGTATGTCTTGCTTTGCATTTGTATGTCTTGGGTGGTACAAGAGTTCTCTTGACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

423

Amino Acids

46.33

Weight (kDa)

8.64

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 662, 668
AclI AACGTT 1 cut(s) 699
AcoI YGGCCR 2 cut(s) 126, 198
AcsI RAATTY 5 cut(s) 31, 415, 505, 547, 850
AcuI CTGAAG 3 cut(s) 170, 962, 1165
AfaI GTAC 1 cut(s) 1246
AfiI CCNNNNNNNGG 1 cut(s) 135
AgsI TTSAA 6 cut(s) 31, 232, 288, 843, 867, 1117
AjnI CCWGG 1 cut(s) 128
AluBI AGCT 7 cut(s) 98, 154, 423, 495, 625, 846, 940
AluI AGCT 7 cut(s) 98, 154, 423, 495, 625, 846, 940
Alw21I GWGCWC 2 cut(s) 627, 1135
Alw26I GTCTC 4 cut(s) 88, 193, 197, 1058
Alw44I GTGCAC 1 cut(s) 1131
AoxI GGCC 2 cut(s) 126, 198
ApaLI GTGCAC 1 cut(s) 1131
ApeKI GCWGC 2 cut(s) 71, 937
ApoI RAATTY 5 cut(s) 31, 415, 505, 547, 850
Asp700I GAANNNNTTC 3 cut(s) 143, 449, 1192
AspS9I GGNCC 1 cut(s) 887
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 3 cut(s) 554, 803, 1066
AvaII GGWCC 1 cut(s) 887
BaeGI GKGCMC 1 cut(s) 1135
BaeI ACNNNNGTAYC 2 cut(s) 1236, 1269
BalI TGGCCA 1 cut(s) 128
BanII GRGCYC 3 cut(s) 15, 166, 627
BbsI GAAGAC 1 cut(s) 1185
Bbv12I GWGCWC 2 cut(s) 627, 1135
BbvI GCAGC 2 cut(s) 83, 949
BccI CCATC 7 cut(s) 48, 178, 580, 582, 713, 975, 1103
BceAI ACGGC 1 cut(s) 474
BciT130I CCWGG 1 cut(s) 130
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 4 cut(s) 88, 193, 197, 1058
BfaI CTAG 1 cut(s) 585
BisI GCNGC 2 cut(s) 72, 938
BlsI GCNGC 2 cut(s) 73, 939
Bme1390I CCNGG 2 cut(s) 130, 469
Bme18I GGWCC 1 cut(s) 887
BmgT120I GGNCC 1 cut(s) 887
BmiI GGNNCC 3 cut(s) 146, 163, 466
BmrFI CCNGG 2 cut(s) 130, 469
BmsI GCATC 2 cut(s) 925, 1089
BpiI GAAGAC 1 cut(s) 1185
BplI GAGNNNNNCTC 6 cut(s) 173, 205, 296, 328, 793, 825
Bpu10I CCTNAGC 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 469
BsaI GGTCTC 2 cut(s) 88, 193
BsaJI CCNNGG 1 cut(s) 336
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse1I ACTGG 1 cut(s) 831
Bse3DI GCAATG 1 cut(s) 784
BseBI CCWGG 1 cut(s) 130
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 5 cut(s) 360, 763, 910, 986, 1104
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMI GCAATG 1 cut(s) 784
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 831
BseRI GAGGAG 1 cut(s) 320
BseSI GKGCMC 1 cut(s) 1135
BseX3I CGGCCG 1 cut(s) 198
BseXI GCAGC 2 cut(s) 83, 949
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 2 cut(s) 128, 200
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 627, 1135
BsiSI CCGG 2 cut(s) 87, 468
BslI CCNNNNNNNGG 1 cut(s) 135
BsmAI GTCTC 4 cut(s) 88, 193, 197, 1058
BsmBI CGTCTC 1 cut(s) 197
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 2 cut(s) 128, 200
Bso31I GGTCTC 2 cut(s) 88, 193
Bsp1286I GDGCHC 4 cut(s) 15, 166, 627, 1135
Bsp143I GATC 2 cut(s) 874, 1073
BspACI CCGC 2 cut(s) 662, 668
BspANI GGCC 2 cut(s) 128, 200
BspCNI CTCAG 1 cut(s) 315
BspLI GGNNCC 3 cut(s) 146, 163, 466
BspTNI GGTCTC 2 cut(s) 88, 193
BsrDI GCAATG 1 cut(s) 784
BsrI ACTGG 1 cut(s) 831
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 2 cut(s) 874, 1073
Bst2UI CCWGG 1 cut(s) 130
Bst4CI ACNGT 4 cut(s) 349, 796, 1069, 1210
Bst6I CTCTTC 2 cut(s) 128, 134
BstC8I GCNNGC 1 cut(s) 914
BstDEI CTNAG 4 cut(s) 21, 323, 501, 1127
BstF5I GGATG 5 cut(s) 360, 763, 910, 986, 1104
BstKTI GATC 2 cut(s) 877, 1076
BstMAI GTCTC 4 cut(s) 88, 193, 197, 1058
BstMBI GATC 2 cut(s) 874, 1073
BstMCI CGRYCG 1 cut(s) 201
BstNI CCWGG 1 cut(s) 130
BstNSI RCATGY 1 cut(s) 860
BstSCI CCNGG 2 cut(s) 128, 467
BstSLI GKGCMC 1 cut(s) 1135
BstV1I GCAGC 2 cut(s) 83, 949
BstV2I GAAGAC 1 cut(s) 1185
BstZI CGGCCG 1 cut(s) 198
BsuRI GGCC 2 cut(s) 128, 200
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 5 cut(s) 360, 763, 910, 986, 1104
BtsI GCAGTG 2 cut(s) 613, 657
BtsIMutI CAGTG 4 cut(s) 320, 613, 657, 1189
Cac8I GCNNGC 1 cut(s) 914
Cfr13I GGNCC 1 cut(s) 887
Csp6I GTAC 1 cut(s) 1245
CviAII CATG 2 cut(s) 857, 1139
CviQI GTAC 1 cut(s) 1245
DdeI CTNAG 4 cut(s) 21, 323, 501, 1127
DpnI GATC 2 cut(s) 876, 1075
DpnII GATC 2 cut(s) 874, 1073
DraI TTTAAA 1 cut(s) 276
EaeI YGGCCR 2 cut(s) 126, 198
EagI CGGCCG 1 cut(s) 198
Eam1104I CTCTTC 2 cut(s) 128, 134
EarI CTCTTC 2 cut(s) 128, 134
Ecl136II GAGCTC 1 cut(s) 625
EclXI CGGCCG 1 cut(s) 198
Eco24I GRGCYC 3 cut(s) 15, 166, 627
Eco31I GGTCTC 2 cut(s) 88, 193
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 1 cut(s) 887
Eco52I CGGCCG 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 625
Eco57I CTGAAG 3 cut(s) 170, 962, 1165
EcoICRI GAGCTC 1 cut(s) 625
EcoRI GAATTC 3 cut(s) 415, 547, 850
EcoRII CCWGG 1 cut(s) 128
EcoRV GATATC 1 cut(s) 694
EcoT38I GRGCYC 3 cut(s) 15, 166, 627
Esp3I CGTCTC 1 cut(s) 197
FaeI CATG 2 cut(s) 860, 1142
FatI CATG 2 cut(s) 856, 1138
FbaI TGATCA 1 cut(s) 874
Fnu4HI GCNGC 2 cut(s) 72, 938
FokI GGATG 5 cut(s) 367, 770, 917, 993, 1111
FriOI GRGCYC 3 cut(s) 15, 166, 627
Fsp4HI GCNGC 2 cut(s) 72, 938
FspBI CTAG 1 cut(s) 585
GluI GCNGC 2 cut(s) 72, 938
HaeIII GGCC 2 cut(s) 128, 200
HapII CCGG 2 cut(s) 87, 468
Hin1II CATG 2 cut(s) 860, 1142
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HindIII AAGCTT 1 cut(s) 493
HinfI GANTC 4 cut(s) 194, 251, 358, 395
HpaII CCGG 2 cut(s) 87, 468
HphI GGTGA 3 cut(s) 554, 803, 1066
Hpy166II GTNNAC 4 cut(s) 109, 673, 726, 1133
Hpy188III TCNNGA 6 cut(s) 148, 181, 442, 920, 1063, 1258
Hpy8I GTNNAC 4 cut(s) 109, 673, 726, 1133
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 6 cut(s) 42, 226, 397, 878, 937, 985
HpyCH4III ACNGT 4 cut(s) 349, 796, 1069, 1210
HpyCH4IV ACGT 2 cut(s) 699, 1058
HpyCH4V TGCA 9 cut(s) 606, 639, 777, 916, 1020, 1133, 1151, 1165, 1225
HpyF3I CTNAG 4 cut(s) 21, 323, 501, 1127
HpySE526I ACGT 2 cut(s) 699, 1058
Hsp92II CATG 2 cut(s) 860, 1142
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 2 cut(s) 874, 1073
LmnI GCTCC 3 cut(s) 95, 161, 622
Lsp1109I GCAGC 2 cut(s) 83, 949
LweI GCATC 2 cut(s) 925, 1089
MaeI CTAG 1 cut(s) 585
MaeII ACGT 2 cut(s) 699, 1058
MaeIII GTNAC 5 cut(s) 63, 318, 1042, 1054, 1204
MalI GATC 2 cut(s) 876, 1075
MboI GATC 2 cut(s) 874, 1073
MboII GAAGA 6 cut(s) 57, 145, 151, 394, 719, 1185
MfeI CAATTG 1 cut(s) 867
MhlI GDGCHC 4 cut(s) 15, 166, 627, 1135
MlsI TGGCCA 1 cut(s) 128
MluNI TGGCCA 1 cut(s) 128
MmeI TCCRAC 2 cut(s) 282, 469
Mox20I TGGCCA 1 cut(s) 128
MroXI GAANNNNTTC 3 cut(s) 143, 449, 1192
MscI TGGCCA 1 cut(s) 128
MseI TTAA 3 cut(s) 275, 594, 1267
MslI CAYNNNNRTG 1 cut(s) 1230
Msp20I TGGCCA 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 940
MspI CCGG 2 cut(s) 87, 468
MspR9I CCNGG 2 cut(s) 130, 469
MunI CAATTG 1 cut(s) 867
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 1 cut(s) 130
NciI CCSGG 1 cut(s) 469
NdeII GATC 2 cut(s) 874, 1073
NlaIII CATG 2 cut(s) 860, 1142
NlaIV GGNNCC 3 cut(s) 146, 163, 466
NmeAIII GCCGAG 1 cut(s) 226
NmuCI GTSAC 3 cut(s) 318, 1042, 1054
NspI RCATGY 1 cut(s) 860
PcsI WCGNNNNNNNCGW 2 cut(s) 203, 557
PctI GAATGC 1 cut(s) 639
PdmI GAANNNNTTC 3 cut(s) 143, 449, 1192
PfeI GAWTC 4 cut(s) 194, 251, 358, 395
PkrI GCNGC 2 cut(s) 73, 939
Psp124BI GAGCTC 1 cut(s) 627
Psp1406I AACGTT 1 cut(s) 699
Psp6I CCWGG 1 cut(s) 128
PspGI CCWGG 1 cut(s) 128
PspN4I GGNNCC 3 cut(s) 146, 163, 466
PspPI GGNCC 1 cut(s) 887
PvuII CAGCTG 1 cut(s) 940
RsaI GTAC 1 cut(s) 1246
RsaNI GTAC 1 cut(s) 1245
RseI CAYNNNNRTG 1 cut(s) 1230
SacI GAGCTC 1 cut(s) 627
SaqAI TTAA 3 cut(s) 275, 594, 1267
SatI GCNGC 2 cut(s) 72, 938
Sau3AI GATC 2 cut(s) 874, 1073
Sau96I GGNCC 1 cut(s) 887
ScrFI CCNGG 2 cut(s) 130, 469
SduI GDGCHC 4 cut(s) 15, 166, 627, 1135
SfaNI GCATC 2 cut(s) 925, 1089
SinI GGWCC 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 1230
SsiI CCGC 2 cut(s) 662, 668
SspMI CTAG 1 cut(s) 585
SstI GAGCTC 1 cut(s) 627
StyD4I CCNGG 2 cut(s) 128, 467
TaaI ACNGT 4 cut(s) 349, 796, 1069, 1210
TaiI ACGT 2 cut(s) 702, 1061
TfiI GAWTC 4 cut(s) 194, 251, 358, 395
Tru1I TTAA 3 cut(s) 275, 594, 1267
Tru9I TTAA 3 cut(s) 275, 594, 1267
TscAI CASTG 4 cut(s) 327, 613, 664, 1189
TseFI GTSAC 3 cut(s) 318, 1042, 1054
TseI GCWGC 2 cut(s) 71, 937
Tsp45I GTSAC 3 cut(s) 318, 1042, 1054
TspDTI ATGAA 5 cut(s) 395, 408, 720, 987, 1127
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 4 cut(s) 327, 613, 664, 1189
VneI GTGCAC 1 cut(s) 1131
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 5 cut(s) 31, 415, 505, 547, 850
XceI RCATGY 1 cut(s) 860
XmnI GAANNNNTTC 3 cut(s) 143, 449, 1192
XspI CTAG 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.