Rh6DG490200

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012893
Physical Location & Seq
Forward (+)
65034959 .. 65036188
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG490200.1

Sequence Viewer

Length: 1230 bp
ATGGAACTCGGGCTCGTCACGAAGGCCTTGCATCGAAGAAAGAAATGGCTTCTGATACTCGCGGCCCTCGGCGCGTCCGGTTACGGCGCCTACAGGGTTTACAGTTCGCCCTCTTTGGTCCAGAAGCGAAAGAGGTTCATGAAGGTCGTCGGAGCCCTAATTTCTATGGCGGAGATGGCGTCTGATTCGGCCGAGACCGTCAGCATTGTCTCCAACGACTTGAAGGAGTTTCTGAACTCCGGTTCCGACGAAATGCCCAAAAGTCTGAAGCAGATTTCCAAGATTGCGAGGTCGGAGGAGTTCTCTGCGTCGTTGCAGAGGTTGACGGAGGCTATGGCGATCGGGGTTTGCCGAGGGTTTGAGTTTGAGACGGGGAATGGGGTTGGATCGGAGAAGGGTCTGGGGAATTCGAGCTATTCGGAGAGAGTTATGGAGAAGGTTTTCTCTGAAGCCGGGACCGGGTTCGTTTCGGTTGTAGTTGGAAGCTTTGCAAGGAATATGGTTATGGGGTTTTCCTCAAATGAGCATTCAGGTCAGGATTCGAGTTCTTCGGATGTGAATAGATGGGTCGATGCGGTTTGTAGTGATCCAAGTAAGGAGCTGATAGCGAATTGCATACAGGTTTTCGTGTCTACTGCGGTTGCGGCGTACCTAGACAAAACGAAGGATGTCAATTTCTGTGATGACATGTTTGGTGGAATGACCAATCCCAAGCATCAGGAGAAGGTCAGGGAGCTCTTGGTTTCTGTTTGCAATGGTGCTGTGGAGACTGTTGTGAAAACTTCTCATCAAGTTTTGACGAGTTCTGGTTCGAATCCGAGCTCAAATTCGGGGACTTGGTCAGTTGTTGAAGGTGTGAGTGGAGCTAGGGATGAGTGCCTTGAGCAAGAAGCAAAGCAGCCGAAGGAGGGAAGTTCACTTGAGGCTATTCAGAATAGCGAGTGGATTGGAAAGGTTTCGTCTACATTGGCAATGCCGCGCAATAGGAAGTTTGTTCGTGAGGTGACTGGGAGGGTGACATTCGAGACTGTAAGATCTTTTGTTGAATTCATGTTGTGGACACTACTGGAGGGGTTGAAAAGAACTGTTAGTGTGGTTCATGGGGAGATTGTGGGTAGGGGGTTGCAAGTTGTCAGATATTTTGGTGCAAAGTCTTCGGCTATTGTTACCATATGTCTTGCTTTGCACTTGTATGTTCTAGGAGGTAGTAGAGTTCTCTTGACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

409

Amino Acids

44.31

Weight (kDa)

8.38

Isoelectric Point (pI)

30.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccI GTMKAC 2 cut(s) 632, 962
AccII CGCG 3 cut(s) 62, 74, 979
AciI CCGC 6 cut(s) 62, 170, 575, 638, 644, 977
AclWI GGATC 2 cut(s) 394, 581
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 3 cut(s) 406, 826, 1046
AcuI CTGAAG 2 cut(s) 287, 468
AcyI GRCGYC 2 cut(s) 87, 179
AfaI GTAC 1 cut(s) 650
AfiI CCNNNNNNNGG 2 cut(s) 459, 908
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 4 cut(s) 223, 851, 1046, 1078
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 6 cut(s) 414, 486, 601, 736, 822, 866
AluI AGCT 6 cut(s) 414, 486, 601, 736, 822, 866
Alw21I GWGCWC 2 cut(s) 738, 824
Alw26I GTCTC 5 cut(s) 188, 214, 362, 761, 1019
AlwI GGATC 2 cut(s) 394, 581
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 3 cut(s) 24, 63, 189
ApeKI GCWGC 1 cut(s) 898
ApoI RAATTY 3 cut(s) 406, 826, 1046
Asp700I GAANNNNTTC 2 cut(s) 440, 955
AspLEI GCGC 3 cut(s) 74, 89, 981
AspS9I GGNCC 3 cut(s) 64, 118, 456
AsuC2I CCSGG 2 cut(s) 454, 460
AsuHPI GGTGA 2 cut(s) 1015, 1027
AsuII TTCGAA 1 cut(s) 812
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 118, 456
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 4 cut(s) 15, 157, 738, 824
BbsI GAAGAC 1 cut(s) 1146
Bbv12I GWGCWC 2 cut(s) 738, 824
BbvI GCAGC 1 cut(s) 910
BccI CCATC 2 cut(s) 169, 558
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 4 cut(s) 10, 44, 1137, 1171
BcnI CCSGG 2 cut(s) 454, 460
BcoDI GTCTC 5 cut(s) 188, 214, 362, 761, 1019
BfaI CTAG 3 cut(s) 653, 867, 1199
BfmI CTRYAG 1 cut(s) 91
BfoI RGCGCY 1 cut(s) 90
BglII AGATCT 1 cut(s) 1034
BisI GCNGC 4 cut(s) 63, 645, 899, 977
BlsI GCNGC 4 cut(s) 64, 646, 900, 978
Bme1390I CCNGG 2 cut(s) 454, 460
Bme18I GGWCC 2 cut(s) 118, 456
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 3 cut(s) 64, 118, 456
BmiI GGNNCC 4 cut(s) 88, 154, 244, 457
BmrFI CCNGG 2 cut(s) 454, 460
BmrI ACTGGG 1 cut(s) 1017
BmsI GCATC 3 cut(s) 40, 562, 724
BmuI ACTGGG 1 cut(s) 1017
BpiI GAAGAC 1 cut(s) 1146
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 1 cut(s) 1088
Bpu14I TTCGAA 1 cut(s) 812
BpuEI CTTGAG 2 cut(s) 902, 941
BpuMI CCSGG 2 cut(s) 454, 460
BsaHI GRCGYC 2 cut(s) 87, 179
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 67, 352
BsaWI WCCGGW 2 cut(s) 77, 239
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 459, 908
Bse1I ACTGG 2 cut(s) 1012, 1071
Bse3DI GCAATG 2 cut(s) 760, 978
BseDI CCNNGG 2 cut(s) 67, 352
BseGI GGATG 3 cut(s) 559, 673, 877
BseLI CCNNNNNNNGG 2 cut(s) 459, 908
BseMI GCAATG 2 cut(s) 760, 978
BseNI ACTGG 2 cut(s) 1012, 1071
BseRI GAGGAG 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 1 cut(s) 910
Bsh1236I CGCG 3 cut(s) 62, 74, 979
Bsh1285I CGRYCG 2 cut(s) 192, 342
BshFI GGCC 3 cut(s) 26, 65, 191
BshNI GGYRCC 1 cut(s) 86
BsiEI CGRYCG 2 cut(s) 192, 342
BsiHKAI GWGCWC 2 cut(s) 738, 824
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 4 cut(s) 78, 240, 453, 459
BslFI GGGAC 2 cut(s) 469, 847
BslI CCNNNNNNNGG 2 cut(s) 459, 908
BsmAI GTCTC 5 cut(s) 188, 214, 362, 761, 1019
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 2 cut(s) 469, 847
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 3 cut(s) 26, 65, 191
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 4 cut(s) 15, 157, 738, 824
Bsp143I GATC 4 cut(s) 339, 386, 586, 1034
BspACI CCGC 6 cut(s) 62, 170, 575, 638, 644, 977
BspANI GGCC 3 cut(s) 26, 65, 191
BspFNI CGCG 3 cut(s) 62, 74, 979
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 4 cut(s) 88, 154, 244, 457
BspPI GGATC 2 cut(s) 394, 581
BspT104I TTCGAA 1 cut(s) 812
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 760, 978
BsrI ACTGG 2 cut(s) 1012, 1071
BssECI CCNNGG 2 cut(s) 67, 352
BssMI GATC 4 cut(s) 339, 386, 586, 1034
BssNI GRCGYC 2 cut(s) 87, 179
Bst4CI ACNGT 5 cut(s) 104, 199, 772, 1030, 1087
BstACI GRCGYC 2 cut(s) 87, 179
BstBI TTCGAA 1 cut(s) 812
BstDEI CTNAG 1 cut(s) 1227
BstF5I GGATG 3 cut(s) 559, 673, 877
BstFNI CGCG 3 cut(s) 62, 74, 979
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 74, 89, 981
BstKTI GATC 4 cut(s) 342, 389, 589, 1037
BstMAI GTCTC 5 cut(s) 188, 214, 362, 761, 1019
BstMBI GATC 4 cut(s) 339, 386, 586, 1034
BstMCI CGRYCG 2 cut(s) 192, 342
BstMWI GCNNNNNNNGC 3 cut(s) 71, 176, 644
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 2 cut(s) 452, 458
BstSFI CTRYAG 1 cut(s) 91
BstUI CGCG 3 cut(s) 62, 74, 979
BstV1I GCAGC 1 cut(s) 910
BstV2I GAAGAC 1 cut(s) 1146
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
BstZI CGGCCG 1 cut(s) 189
BsuRI GGCC 3 cut(s) 26, 65, 191
BtsCI GGATG 3 cut(s) 559, 673, 877
CciI TCATGA 1 cut(s) 138
CfoI GCGC 3 cut(s) 74, 89, 981
Cfr13I GGNCC 3 cut(s) 64, 118, 456
CseI GACGC 3 cut(s) 63, 168, 297
Csp6I GTAC 1 cut(s) 649
CviAII CATG 4 cut(s) 139, 688, 1051, 1100
CviQI GTAC 1 cut(s) 649
DdeI CTNAG 1 cut(s) 1227
DinI GGCGCC 1 cut(s) 88
DpnI GATC 4 cut(s) 341, 388, 588, 1036
DpnII GATC 4 cut(s) 339, 386, 586, 1034
EaeI YGGCCR 1 cut(s) 189
EagI CGGCCG 1 cut(s) 189
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 2 cut(s) 736, 822
EclXI CGGCCG 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 4 cut(s) 15, 157, 738, 824
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 118, 456
Eco52I CGGCCG 1 cut(s) 189
Eco53kI GAGCTC 2 cut(s) 736, 822
Eco57I CTGAAG 2 cut(s) 287, 468
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 736, 822
EcoRI GAATTC 2 cut(s) 406, 1046
EcoT38I GRGCYC 4 cut(s) 15, 157, 738, 824
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 4 cut(s) 142, 691, 1054, 1103
FaqI GGGAC 2 cut(s) 469, 847
FatI CATG 4 cut(s) 138, 687, 1050, 1099
FauNDI CATATG 1 cut(s) 1172
FblI GTMKAC 2 cut(s) 632, 962
Fnu4HI GCNGC 4 cut(s) 63, 645, 899, 977
FokI GGATG 3 cut(s) 566, 680, 884
FriOI GRGCYC 4 cut(s) 15, 157, 738, 824
Fsp4HI GCNGC 4 cut(s) 63, 645, 899, 977
FspBI CTAG 3 cut(s) 653, 867, 1199
GlaI GCGC 3 cut(s) 73, 88, 980
GluI GCNGC 4 cut(s) 63, 645, 899, 977
GsuI CTGGAG 1 cut(s) 1088
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 3 cut(s) 26, 65, 191
HapII CCGG 4 cut(s) 78, 240, 453, 459
HgaI GACGC 3 cut(s) 63, 168, 297
HhaI GCGC 3 cut(s) 74, 89, 981
Hin1I GRCGYC 2 cut(s) 87, 179
Hin1II CATG 4 cut(s) 142, 691, 1054, 1103
Hin6I GCGC 3 cut(s) 72, 87, 979
HinP1I GCGC 3 cut(s) 72, 87, 979
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 3 cut(s) 185, 539, 814
HpaII CCGG 4 cut(s) 78, 240, 453, 459
HphI GGTGA 2 cut(s) 1015, 1027
Hpy166II GTNNAC 6 cut(s) 100, 324, 633, 917, 963, 1059
Hpy188III TCNNGA 8 cut(s) 19, 121, 139, 536, 719, 998, 1024, 1219
Hpy8I GTNNAC 6 cut(s) 100, 324, 633, 917, 963, 1059
Hpy99I CGWCG 3 cut(s) 152, 251, 313
HpyAV CCTTC 9 cut(s) 16, 136, 217, 388, 430, 658, 718, 845, 898
HpyCH4III ACNGT 5 cut(s) 104, 199, 772, 1030, 1087
HpyCH4V TGCA 8 cut(s) 31, 316, 491, 615, 753, 1126, 1148, 1186
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 176, 644
HpyF3I CTNAG 1 cut(s) 1227
Hsp92I GRCGYC 2 cut(s) 87, 179
Hsp92II CATG 4 cut(s) 142, 691, 1054, 1103
HspAI GCGC 3 cut(s) 72, 87, 979
KasI GGCGCC 1 cut(s) 86
Kzo9I GATC 4 cut(s) 339, 386, 586, 1034
LmnI GCTCC 4 cut(s) 152, 598, 733, 863
Lsp1109I GCAGC 1 cut(s) 910
LweI GCATC 3 cut(s) 40, 562, 724
MaeI CTAG 3 cut(s) 653, 867, 1199
MaeIII GTNAC 5 cut(s) 16, 80, 1003, 1015, 1165
MalI GATC 4 cut(s) 341, 388, 588, 1036
MboI GATC 4 cut(s) 339, 386, 586, 1034
MboII GAAGA 3 cut(s) 48, 540, 1146
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 4 cut(s) 15, 157, 738, 824
MluCI AATT 6 cut(s) 159, 406, 610, 673, 826, 1046
Mly113I GGCGCC 1 cut(s) 87
MmeI TCCRAC 6 cut(s) 130, 237, 270, 273, 364, 460
MroXI GAANNNNTTC 2 cut(s) 440, 955
MslI CAYNNNNRTG 1 cut(s) 1191
MspI CCGG 4 cut(s) 78, 240, 453, 459
MspR9I CCNGG 2 cut(s) 454, 460
Mva1269I GAATGC 1 cut(s) 526
MvnI CGCG 3 cut(s) 62, 74, 979
MwoI GCNNNNNNNGC 3 cut(s) 71, 176, 644
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 2 cut(s) 454, 460
NdeI CATATG 1 cut(s) 1172
NdeII GATC 4 cut(s) 339, 386, 586, 1034
NlaIII CATG 4 cut(s) 142, 691, 1054, 1103
NlaIV GGNNCC 4 cut(s) 88, 154, 244, 457
NmeAIII GCCGAG 3 cut(s) 48, 217, 377
NmuCI GTSAC 3 cut(s) 16, 1003, 1015
NspI RCATGY 1 cut(s) 691
NspV TTCGAA 1 cut(s) 812
PagI TCATGA 1 cut(s) 138
PceI AGGCCT 1 cut(s) 26
PciI ACATGT 1 cut(s) 687
PctI GAATGC 1 cut(s) 526
PdmI GAANNNNTTC 2 cut(s) 440, 955
PfeI GAWTC 3 cut(s) 185, 539, 814
PflFI GACNNNGTC 1 cut(s) 838
PkrI GCNGC 4 cut(s) 64, 646, 900, 978
Ple19I CGATCG 1 cut(s) 342
PluTI GGCGCC 1 cut(s) 90
PscI ACATGT 1 cut(s) 687
Psp124BI GAGCTC 2 cut(s) 738, 824
PspN4I GGNNCC 4 cut(s) 88, 154, 244, 457
PspPI GGNCC 3 cut(s) 64, 118, 456
PsuI RGATCY 1 cut(s) 1034
PsyI GACNNNGTC 1 cut(s) 838
PvuI CGATCG 1 cut(s) 342
RsaI GTAC 1 cut(s) 650
RsaNI GTAC 1 cut(s) 649
RseI CAYNNNNRTG 1 cut(s) 1191
SacI GAGCTC 2 cut(s) 738, 824
SatI GCNGC 4 cut(s) 63, 645, 899, 977
Sau3AI GATC 4 cut(s) 339, 386, 586, 1034
Sau96I GGNCC 3 cut(s) 64, 118, 456
ScrFI CCNGG 2 cut(s) 454, 460
SduI GDGCHC 4 cut(s) 15, 157, 738, 824
SfaNI GCATC 3 cut(s) 40, 562, 724
SfcI CTRYAG 1 cut(s) 91
SfoI GGCGCC 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 2 cut(s) 118, 456
SmiMI CAYNNNNRTG 1 cut(s) 1191
SmlI CTYRAG 2 cut(s) 881, 920
SmoI CTYRAG 2 cut(s) 881, 920
Sse9I AATT 6 cut(s) 159, 406, 610, 673, 826, 1046
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 6 cut(s) 62, 170, 575, 638, 644, 977
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 3 cut(s) 653, 867, 1199
SstI GAGCTC 2 cut(s) 738, 824
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 452, 458
TaaI ACNGT 5 cut(s) 104, 199, 772, 1030, 1087
TaqI TCGA 6 cut(s) 34, 410, 542, 570, 812, 1023
TasI AATT 6 cut(s) 159, 406, 610, 673, 826, 1046
TauI GCSGC 3 cut(s) 65, 647, 979
TfiI GAWTC 3 cut(s) 185, 539, 814
TseFI GTSAC 3 cut(s) 16, 1003, 1015
TseI GCWGC 1 cut(s) 898
Tsp45I GTSAC 3 cut(s) 16, 1003, 1015
TspDTI ATGAA 4 cut(s) 127, 155, 1039, 1088
TspGWI ACGGA 1 cut(s) 341
Tth111I GACNNNGTC 1 cut(s) 838
VpaK11BI GGWCC 2 cut(s) 118, 456
XapI RAATTY 3 cut(s) 406, 826, 1046
XceI RCATGY 1 cut(s) 691
XmiI GTMKAC 2 cut(s) 632, 962
XmnI GAANNNNTTC 2 cut(s) 440, 955
XspI CTAG 3 cut(s) 653, 867, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.