Rorug06G0375800

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010000
Physical Location & Seq
Forward (+)
52265546 .. 52268752
3207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0375800.1

Sequence Viewer

Length: 1455 bp
ATGGGTGATAACAAGAACCAGATGCTTCAACACAACCAAGTATTTGATGTCTCAGTCAATATGCCGCCACAGGGAGGCTCCAAGTGCTTTGATGATGATGGCCGTCTCAAAAGAACCGGAACTGTTTGGACTGCAAGTGCTCACATAATTACTGCTGTAATTGGGTCTGGAGTTCTATCCTTGGCTTGGGCCATAGCTCAGCTTGGATGGGTGGCTGGCCCTGCTGTGATGTTCTTGTTCTCTTTGGTGACTTACTACACTTCAACTCTGCTCTCTGCTTGCTACCGCTCCAATGATCCTGACACTGGAAAAAGGAACTACACTTACATGGATGCTGTTCAATCTAACCTAGGTGGAGCTAAGGTCAAGATTTGTGGATATGTTCAGTACTTTAACCTTTTTGGAGTTGCCATTGGATACACTATAGCATCATCTATTAGCATGATGGCAATCAAGAGGTCTAACTGCTTCCACAAAAGTGGTGGAAAAGCTCCATGCCATGTCAACAGCAACCCCTACATGATTGCATTTGGCATAGCAGAAATTATATTCTCTCAGATTCCAGACTTTGATCAGTTGTGGTGGCTTTCCATTGTTGCTGCAGTCATGTCCTTCACTTACTCCACAATTGGACTTGCCCTTGGAATTGCTAAAGTTGCAGAAACTGGAACAATCATGGGAAGTATGACCGGAATAAGCATTGGCACTGTGACTGAAACCCAAAAGATGTGGAGGAGCTTCCAAGCTCTTGGTGACATAGCTTTTGCCTACTCTTACTCTCTTATTTTGATTGAAATTCAGGACACAGTTAGATCTCCACCATCTGAAGCCAAGACAATGAAGAAGGCCACTATAATTAGTGTTGCAGTGACAACCCTTTTCTACATGCTATGTGGCTGCATGGGCTATGCTGCTTTTGGAGACTTATCCCCTGGAAACTTGCTCACTGGTTTTGGCTTCTATAACCCATATTGGCTCTTAGACATTGCCAACGTTGCTATAGTAGTCCACCTTGTTGGTGCATACCAAGTTTACTGCCAACCCTTATTTGCCTTTGTTGAGAAAGCAGCAGCGAAAAGGTATCCGGAGAGCGAATTTATCTCAAGAGAAATCAAAATCCCAATTCCCGGTCTTGGCCATTGCAATCTTAACCTCTTCAGATTGGTGTGGAGGACTGCCTTTGTGATTGTGACCACTGTAATCTCTATGCTCCTCCCATTCTTTAATGATGTGGTTGGACTTCTTGGAGCTTTGGGATTCTGGCCATTGACTGTTTACTTCCCAGTTGAGATGTACATTGTGCAGAAGAGGATACCAAAGTGGAGCACAAGATGGCTTTGCCTCCAAATCCTAAGTGTTGCTTGCCTTATAATAACCATAGCTGCTGCTGCTGGCTCAATTGCTGGGGTGATTTCTGACCTCAAGATCTACAAGCCATTCAAGACCAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

484

Amino Acids

53.06

Weight (kDa)

8.89

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 38 - 471 1.7e-125 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1370
AccBSI CCGCTC 1 cut(s) 288
AccIII TCCGGA 1 cut(s) 1084
AciI CCGC 2 cut(s) 65, 286
AclI AACGTT 1 cut(s) 993
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 3 cut(s) 100, 1135, 1262
AcsI RAATTY 2 cut(s) 795, 1094
AcuI CTGAAG 2 cut(s) 846, 1141
AfaI GTAC 2 cut(s) 389, 1295
AfiI CCNNNNNNNGG 6 cut(s) 71, 74, 186, 305, 1127, 1133
AgsI TTSAA 5 cut(s) 29, 264, 341, 794, 1441
AjnI CCWGG 1 cut(s) 931
AleI CACNNNNGTG 1 cut(s) 477
Alw21I GWGCWC 2 cut(s) 142, 1328
Alw26I GTCTC 3 cut(s) 55, 110, 915
AlwI GGATC 1 cut(s) 290
AlwNI CAGNNNCTG 1 cut(s) 665
Aor13HI TCCGGA 1 cut(s) 1084
AoxI GGCC 6 cut(s) 100, 189, 217, 846, 1135, 1262
ApeKI GCWGC 8 cut(s) 599, 897, 911, 1067, 1070, 1382, 1385, 1388
ApoI RAATTY 2 cut(s) 795, 1094
ArsI GACNNNNNNTTYG 2 cut(s) 746, 778
AspA2I CCTAGG 1 cut(s) 349
AspS9I GGNCC 2 cut(s) 189, 218
AsuC2I CCSGG 1 cut(s) 1128
AsuHPI GGTGA 4 cut(s) 17, 259, 764, 1420
AvrII CCTAGG 1 cut(s) 349
BaeI ACNNNNGTAYC 2 cut(s) 1304, 1337
BalI TGGCCA 2 cut(s) 1137, 1264
Bbv12I GWGCWC 2 cut(s) 142, 1328
BbvI GCAGC 8 cut(s) 586, 884, 898, 1079, 1082, 1369, 1372, 1375
BccI CCATC 5 cut(s) 92, 201, 439, 829, 1326
BceAI ACGGC 1 cut(s) 87
BciT130I CCWGG 1 cut(s) 933
BciVI GTATCC 3 cut(s) 410, 1092, 1305
BclI TGATCA 1 cut(s) 571
BcnI CCSGG 1 cut(s) 1128
BcoDI GTCTC 3 cut(s) 55, 110, 915
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 3 cut(s) 423, 600, 999
BfuI GTATCC 3 cut(s) 410, 1092, 1305
BglII AGATCT 2 cut(s) 812, 1425
BisI GCNGC 9 cut(s) 65, 600, 898, 912, 1068, 1071, 1383, 1386, 1389
BlnI CCTAGG 1 cut(s) 349
BlpI GCTNAGC 1 cut(s) 198
BlsI GCNGC 9 cut(s) 66, 601, 899, 913, 1069, 1072, 1384, 1387, 1390
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 2 cut(s) 933, 1128
BmgT120I GGNCC 2 cut(s) 189, 218
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 2 cut(s) 933, 1128
BmrI ACTGGG 1 cut(s) 1277
BmsI GCATC 3 cut(s) 12, 322, 437
BmuI ACTGGG 1 cut(s) 1277
BpmI CTGGAG 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 360
Bpu1102I GCTNAGC 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 1087, 1406
BpuMI CCSGG 1 cut(s) 1128
BsaBI GATNNNNATC 1 cut(s) 449
BsaJI CCNNGG 4 cut(s) 180, 349, 640, 931
BsaWI WCCGGW 3 cut(s) 116, 689, 1084
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 74, 186, 305, 1127, 1133
Bse1I ACTGG 4 cut(s) 310, 670, 952, 1283
Bse3DI GCAATG 2 cut(s) 984, 1138
Bse8I GATNNNNATC 1 cut(s) 449
BseAI TCCGGA 1 cut(s) 1084
BseBI CCWGG 1 cut(s) 933
BseDI CCNNGG 4 cut(s) 180, 349, 640, 931
BseGI GGATG 2 cut(s) 212, 337
BseJI GATNNNNATC 1 cut(s) 449
BseLI CCNNNNNNNGG 6 cut(s) 71, 74, 186, 305, 1127, 1133
BseMI GCAATG 2 cut(s) 984, 1138
BseMII CTCAG 3 cut(s) 66, 212, 569
BseNI ACTGG 4 cut(s) 310, 670, 952, 1283
BseRI GAGGAG 2 cut(s) 748, 1202
BseXI GCAGC 8 cut(s) 586, 884, 898, 1079, 1082, 1369, 1372, 1375
BseYI CCCAGC 1 cut(s) 1403
BsgI GTGCAG 1 cut(s) 1322
BshFI GGCC 6 cut(s) 102, 191, 219, 848, 1137, 1264
BsiHKAI GWGCWC 2 cut(s) 142, 1328
BsiSI CCGG 4 cut(s) 117, 690, 1085, 1128
BslI CCNNNNNNNGG 6 cut(s) 71, 74, 186, 305, 1127, 1133
BsmAI GTCTC 3 cut(s) 55, 110, 915
BsmBI CGTCTC 1 cut(s) 110
BsnI GGCC 6 cut(s) 102, 191, 219, 848, 1137, 1264
Bsp1286I GDGCHC 2 cut(s) 142, 1328
Bsp13I TCCGGA 1 cut(s) 1084
Bsp1407I TGTACA 1 cut(s) 1293
Bsp143I GATC 4 cut(s) 295, 571, 812, 1425
Bsp1720I GCTNAGC 1 cut(s) 198
BspACI CCGC 2 cut(s) 65, 286
BspANI GGCC 6 cut(s) 102, 191, 219, 848, 1137, 1264
BspCNI CTCAG 3 cut(s) 65, 211, 568
BspEI TCCGGA 1 cut(s) 1084
BspLI GGNNCC 1 cut(s) 79
BspMAI CTGCAG 1 cut(s) 604
BspPI GGATC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 984, 1138
BsrGI TGTACA 1 cut(s) 1293
BsrI ACTGG 4 cut(s) 310, 670, 952, 1283
BssECI CCNNGG 4 cut(s) 180, 349, 640, 931
BssMI GATC 4 cut(s) 295, 571, 812, 1425
BssT1I CCWWGG 3 cut(s) 180, 349, 640
Bst2UI CCWGG 1 cut(s) 933
Bst4CI ACNGT 5 cut(s) 124, 709, 808, 1198, 1273
Bst6I CTCTTC 2 cut(s) 1160, 1301
BstAUI TGTACA 1 cut(s) 1293
BstC8I GCNNGC 4 cut(s) 217, 280, 1363, 1393
BstDEI CTNAG 6 cut(s) 52, 198, 360, 555, 979, 1352
BstF5I GGATG 2 cut(s) 212, 337
BstKTI GATC 4 cut(s) 298, 574, 815, 1428
BstMAI GTCTC 3 cut(s) 55, 110, 915
BstMBI GATC 4 cut(s) 295, 571, 812, 1425
BstMWI GCNNNNNNNGC 6 cut(s) 84, 221, 656, 903, 995, 1388
BstNI CCWGG 1 cut(s) 933
BstNSI RCATGY 1 cut(s) 889
BstSCI CCNGG 2 cut(s) 931, 1126
BstSFI CTRYAG 3 cut(s) 423, 600, 999
BstV1I GCAGC 8 cut(s) 586, 884, 898, 1079, 1082, 1369, 1372, 1375
BstX2I RGATCY 2 cut(s) 812, 1425
BstXI CCANNNNNNTGG 3 cut(s) 479, 749, 1016
BstYI RGATCY 2 cut(s) 812, 1425
BsuI GTATCC 3 cut(s) 410, 1092, 1305
BsuRI GGCC 6 cut(s) 102, 191, 219, 848, 1137, 1264
BtsCI GGATG 2 cut(s) 212, 337
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 5 cut(s) 303, 705, 873, 945, 1194
Cac8I GCNNGC 4 cut(s) 217, 280, 1363, 1393
CaiI CAGNNNCTG 1 cut(s) 665
Cfr13I GGNCC 2 cut(s) 189, 218
Csp6I GTAC 2 cut(s) 388, 1294
CspCI CAANNNNNGTGG 6 cut(s) 355, 390, 463, 498, 710, 745
CviAII CATG 9 cut(s) 328, 442, 495, 500, 520, 607, 676, 886, 901
CviQI GTAC 2 cut(s) 388, 1294
DdeI CTNAG 6 cut(s) 52, 198, 360, 555, 979, 1352
DpnI GATC 4 cut(s) 297, 573, 814, 1427
DpnII GATC 4 cut(s) 295, 571, 812, 1425
EaeI YGGCCR 3 cut(s) 100, 1135, 1262
Eam1104I CTCTTC 2 cut(s) 1160, 1301
EarI CTCTTC 2 cut(s) 1160, 1301
Eco130I CCWWGG 3 cut(s) 180, 349, 640
Eco57I CTGAAG 2 cut(s) 846, 1141
EcoRII CCWGG 1 cut(s) 931
EcoT14I CCWWGG 3 cut(s) 180, 349, 640
ErhI CCWWGG 3 cut(s) 180, 349, 640
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 9 cut(s) 331, 445, 498, 503, 523, 610, 679, 889, 904
FalI AAGNNNNNCTT 2 cut(s) 1345, 1377
FatI CATG 9 cut(s) 327, 441, 494, 499, 519, 606, 675, 885, 900
FbaI TGATCA 1 cut(s) 571
Fnu4HI GCNGC 9 cut(s) 65, 600, 898, 912, 1068, 1071, 1383, 1386, 1389
FokI GGATG 2 cut(s) 219, 344
Fsp4HI GCNGC 9 cut(s) 65, 600, 898, 912, 1068, 1071, 1383, 1386, 1389
FspBI CTAG 1 cut(s) 350
GluI GCNGC 9 cut(s) 65, 600, 898, 912, 1068, 1071, 1383, 1386, 1389
GsaI CCCAGC 1 cut(s) 1407
GsuI CTGGAG 1 cut(s) 189
HaeIII GGCC 6 cut(s) 102, 191, 219, 848, 1137, 1264
HapII CCGG 4 cut(s) 117, 690, 1085, 1128
Hin1II CATG 9 cut(s) 331, 445, 498, 503, 523, 610, 679, 889, 904
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 2 cut(s) 559, 1257
HpaII CCGG 4 cut(s) 117, 690, 1085, 1128
HphI GGTGA 4 cut(s) 17, 259, 764, 1420
Hpy166II GTNNAC 4 cut(s) 505, 1009, 1033, 1276
Hpy188I TCNGA 4 cut(s) 558, 826, 1160, 1417
Hpy8I GTNNAC 4 cut(s) 505, 1009, 1033, 1276
HpyAV CCTTC 2 cut(s) 622, 838
HpyCH4III ACNGT 5 cut(s) 124, 709, 808, 1198, 1273
HpyCH4IV ACGT 1 cut(s) 993
HpyCH4V TGCA 9 cut(s) 134, 527, 602, 659, 866, 900, 1022, 1143, 1303
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 221, 656, 903, 995, 1388
HpyF3I CTNAG 6 cut(s) 52, 198, 360, 555, 979, 1352
HpySE526I ACGT 1 cut(s) 993
Hsp92II CATG 9 cut(s) 331, 445, 498, 503, 523, 610, 679, 889, 904
Kpn2I TCCGGA 1 cut(s) 1084
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 4 cut(s) 295, 571, 812, 1425
LmnI GCTCC 8 cut(s) 83, 293, 356, 496, 735, 1215, 1247, 1323
Lsp1109I GCAGC 8 cut(s) 586, 884, 898, 1079, 1082, 1369, 1372, 1375
LweI GCATC 3 cut(s) 12, 322, 437
MaeI CTAG 1 cut(s) 350
MaeII ACGT 1 cut(s) 993
MaeIII GTNAC 5 cut(s) 247, 709, 752, 868, 1189
MalI GATC 4 cut(s) 297, 573, 814, 1427
MbiI CCGCTC 1 cut(s) 288
MboI GATC 4 cut(s) 295, 571, 812, 1425
MboII GAAGA 3 cut(s) 853, 1147, 1318
MfeI CAATTG 2 cut(s) 627, 1398
MflI RGATCY 2 cut(s) 812, 1425
MhlI GDGCHC 2 cut(s) 142, 1328
MlsI TGGCCA 2 cut(s) 1137, 1264
MluNI TGGCCA 2 cut(s) 1137, 1264
MmeI TCCRAC 1 cut(s) 1216
MnlI CCTC 9 cut(s) 68, 450, 726, 1163, 1164, 1223, 1302, 1352, 1430
Mox20I TGGCCA 2 cut(s) 1137, 1264
MroI TCCGGA 1 cut(s) 1084
MscI TGGCCA 2 cut(s) 1137, 1264
MseI TTAA 3 cut(s) 393, 1149, 1224
MslI CAYNNNNRTG 2 cut(s) 326, 477
Msp20I TGGCCA 2 cut(s) 1137, 1264
MspI CCGG 4 cut(s) 117, 690, 1085, 1128
MspR9I CCNGG 2 cut(s) 933, 1128
MunI CAATTG 2 cut(s) 627, 1398
MvaI CCWGG 1 cut(s) 933
MwoI GCNNNNNNNGC 6 cut(s) 84, 221, 656, 903, 995, 1388
NciI CCSGG 1 cut(s) 1128
NdeII GATC 4 cut(s) 295, 571, 812, 1425
NlaIII CATG 9 cut(s) 331, 445, 498, 503, 523, 610, 679, 889, 904
NlaIV GGNNCC 1 cut(s) 79
NmuCI GTSAC 5 cut(s) 247, 709, 752, 868, 1189
NspI RCATGY 1 cut(s) 889
OliI CACNNNNGTG 1 cut(s) 477
PfeI GAWTC 2 cut(s) 559, 1257
PkrI GCNGC 9 cut(s) 66, 601, 899, 913, 1069, 1072, 1384, 1387, 1390
PsiI TTATAA 1 cut(s) 1370
Psp1406I AACGTT 1 cut(s) 993
Psp6I CCWGG 1 cut(s) 931
PspFI CCCAGC 1 cut(s) 1403
PspGI CCWGG 1 cut(s) 931
PspN4I GGNNCC 1 cut(s) 79
PspPI GGNCC 2 cut(s) 189, 218
PsrI GAACNNNNNNTAC 2 cut(s) 308, 340
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 1 cut(s) 665
PsuI RGATCY 2 cut(s) 812, 1425
RsaI GTAC 2 cut(s) 389, 1295
RsaNI GTAC 2 cut(s) 388, 1294
RseI CAYNNNNRTG 2 cut(s) 326, 477
SaqAI TTAA 3 cut(s) 393, 1149, 1224
SatI GCNGC 9 cut(s) 65, 600, 898, 912, 1068, 1071, 1383, 1386, 1389
Sau3AI GATC 4 cut(s) 295, 571, 812, 1425
Sau96I GGNCC 2 cut(s) 189, 218
ScaI AGTACT 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 933, 1128
SduI GDGCHC 2 cut(s) 142, 1328
SfaNI GCATC 3 cut(s) 12, 322, 437
SfcI CTRYAG 3 cut(s) 423, 600, 999
SmiMI CAYNNNNRTG 2 cut(s) 326, 477
SmlI CTYRAG 2 cut(s) 1102, 1421
SmoI CTYRAG 2 cut(s) 1102, 1421
SsiI CCGC 2 cut(s) 65, 286
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 931, 1126
StyI CCWWGG 3 cut(s) 180, 349, 640
TaaI ACNGT 5 cut(s) 124, 709, 808, 1198, 1273
TaiI ACGT 1 cut(s) 996
TatI WGTACW 2 cut(s) 387, 1293
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 2 cut(s) 559, 1257
Tru1I TTAA 3 cut(s) 393, 1149, 1224
Tru9I TTAA 3 cut(s) 393, 1149, 1224
TscAI CASTG 5 cut(s) 310, 712, 873, 952, 1201
TseFI GTSAC 5 cut(s) 247, 709, 752, 868, 1189
TseI GCWGC 8 cut(s) 599, 897, 911, 1067, 1070, 1382, 1385, 1388
Tsp45I GTSAC 5 cut(s) 247, 709, 752, 868, 1189
TspDTI ATGAA 1 cut(s) 854
TspRI CASTG 5 cut(s) 310, 712, 873, 952, 1201
XapI RAATTY 2 cut(s) 795, 1094
XceI RCATGY 1 cut(s) 889
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XmaJI CCTAGG 1 cut(s) 349
XspI CTAG 1 cut(s) 350
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.