Rh6AG489600

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010000
Physical Location & Seq
Forward (+)
66645632 .. 66648414
2783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG489600.1

Sequence Viewer

Length: 1242 bp
ATGTCATGGCTCGCCCACTCGCTCTCTAAATCCCTCCGTATCGACGGCGACGGCGGCGACGGCGAAGGCCAAGATCACGACGACGACGTCGTACCCCACCACCACACCAACAATGAAGCTGACGTCCCGCCCACCGCCTCCACCAAACCCCAAGACAACGAAATCCAATCGGATGCAGAAGCAGTTGAAGACGCCCAAACGCGTGGCGTGAAAGAAGACCTGTCGGAATTCCAGCAGACCCTAACGCGCCAATTCTGGGGCGTCGCCTCGTTTCTTGCGCCACCACCGCCCTCTGATCGATTAGCTTCTCCGCCCGATTGCGATCGATCCGAGCCGGCCGATCACCCACCCGGTCAATCGCCGATGGCGTTGAATGAAGACGAAGAAGAGGAGGAGGAGGAGGAGGAGGAGTGCGCCGTCGGTGTCACTGACGAGGTCTTGGCCTTCGCTAGAAACATCGCGATGCATCCAGAGACCTGGTTGGACTTTCCTCTTGATGAAGAAGAAGACTTGGGAGATTTTGAAATGTCTGATGCCCAGCAAGAGCATGCTATGATGATTGAACATCTTGCGCCGAGATTAGCTGCTTTGAGAATCGAGCTTTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAACAAGCAGGATGCTGAGGTTTTGTCCACACCTCAGGTAATGGAAGCCAGAGCAATGTGGATGCAAGAGCTACAATGTCAGACTCCAACATCAATTTCTAAAATGTCAGAACATGAATGGTTTGGAAGAAGCACTTCACATTTAAAATACAGTTCAAATGCAATACATGAAGATTATGAAGATTACTCTCCGGTCTCATCACATACTCCTCATTATGAGGATACACTTCATAAGACGTATCCTTTTGAATCAACCATGCCTTCCACAGCCGATGAGACTGAGAAGCACCCAGTTGAGAGTAATGAGATGCCATTCATTGATAAGTCCGTTATCGAAGAAAAGCCCTTCATTAGGACTGAGGATAAGAATGTGACAGTCGGTCCATCCTCTGAGATACTAGTTAATAGCTATGACGACGACGACGATGATGACTGGCCAGAGGATGATGGTGAATTATATGGGTGTAGTGGAACAGCTGTTGCTTTGATAAATGAAGAAGACATATCTTTTAGTGATCTTGAGGATGATGATTTCCAGGCACCCTCTAAATTAAAGTTCATCTCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

413

Amino Acids

46.68

Weight (kDa)

4.22

Isoelectric Point (pI)

70.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 172 - 227 1.7e-13 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 2 cut(s) 90, 126
AccB1I GGYRCC 1 cut(s) 1207
AccII CGCG 3 cut(s) 202, 247, 461
AciI CCGC 5 cut(s) 54, 128, 135, 287, 311
AclWI GGATC 1 cut(s) 321
AcoI YGGCCR 2 cut(s) 336, 1103
AcsI RAATTY 1 cut(s) 227
AcyI GRCGYC 4 cut(s) 87, 123, 192, 261
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 256, 1020
AflIII ACRYGT 1 cut(s) 200
AgsI TTSAA 6 cut(s) 188, 373, 524, 563, 825, 917
AhdI GACNNNNNGTC 1 cut(s) 1047
AhlI ACTAGT 1 cut(s) 1066
AjnI CCWGG 2 cut(s) 476, 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 748, 780
AluBI AGCT 7 cut(s) 119, 305, 584, 601, 739, 1077, 1145
AluI AGCT 7 cut(s) 119, 305, 584, 601, 739, 1077, 1145
Alw26I GTCTC 3 cut(s) 467, 868, 938
AlwI GGATC 1 cut(s) 321
AoxI GGCC 4 cut(s) 67, 336, 441, 1103
ApeKI GCWGC 1 cut(s) 584
ApoI RAATTY 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 802
AspLEI GCGC 4 cut(s) 249, 280, 416, 574
AspS9I GGNCC 1 cut(s) 1049
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 2 cut(s) 335, 1130
AvaII GGWCC 1 cut(s) 1049
AxyI CCTNAGG 1 cut(s) 702
BalI TGGCCA 1 cut(s) 1105
BanI GGYRCC 1 cut(s) 1207
BbsI GAAGAC 5 cut(s) 195, 222, 384, 513, 1173
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 1 cut(s) 571
BccI CCATC 3 cut(s) 358, 1060, 1109
BceAI ACGGC 4 cut(s) 61, 67, 76, 401
BciT130I CCWGG 2 cut(s) 478, 1205
BciVI GTATCC 2 cut(s) 883, 918
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 3 cut(s) 467, 868, 938
BcuI ACTAGT 1 cut(s) 1066
BfaI CTAG 2 cut(s) 450, 1067
BfuI GTATCC 2 cut(s) 883, 918
BisI GCNGC 2 cut(s) 55, 585
BlsI GCNGC 2 cut(s) 56, 586
Bme1390I CCNGG 3 cut(s) 351, 478, 1205
Bme18I GGWCC 1 cut(s) 1049
BmeRI GACNNNNNGTC 1 cut(s) 1047
BmgT120I GGNCC 1 cut(s) 1049
BmiI GGNNCC 1 cut(s) 1209
BmrFI CCNGG 3 cut(s) 351, 478, 1205
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 7 cut(s) 163, 453, 475, 523, 670, 720, 966
BmuI ACTGGG 1 cut(s) 953
BpiI GAAGAC 5 cut(s) 195, 222, 384, 513, 1173
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 1208
BpuMI CCSGG 1 cut(s) 351
Bsa29I ATCGAT 2 cut(s) 298, 325
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 4 cut(s) 87, 123, 192, 261
BsaI GGTCTC 2 cut(s) 467, 868
BsaWI WCCGGW 1 cut(s) 859
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 1020
Bse118I RCCGGY 1 cut(s) 334
Bse1I ACTGG 2 cut(s) 959, 1106
Bse21I CCTNAGG 1 cut(s) 702
Bse3DI GCAATG 1 cut(s) 729
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 2 cut(s) 478, 1205
BseCI ATCGAT 2 cut(s) 298, 325
BseGI GGATG 7 cut(s) 178, 466, 685, 735, 1052, 1117, 1198
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 256, 1020
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 5 cut(s) 675, 716, 939, 1017, 1050
BseNI ACTGG 2 cut(s) 959, 1106
BseRI GAGGAG 8 cut(s) 404, 407, 410, 413, 416, 419, 422, 867
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 571
BseYI CCCAGC 1 cut(s) 537
Bsh1236I CGCG 3 cut(s) 202, 247, 461
Bsh1285I CGRYCG 2 cut(s) 325, 339
BshFI GGCC 4 cut(s) 69, 338, 443, 1105
BshNI GGYRCC 1 cut(s) 1207
BshVI ATCGAT 2 cut(s) 298, 325
BsiEI CGRYCG 2 cut(s) 325, 339
BsiSI CCGG 3 cut(s) 335, 351, 860
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 256, 1020
BsmAI GTCTC 3 cut(s) 467, 868, 938
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 4 cut(s) 69, 338, 443, 1105
Bso31I GGTCTC 2 cut(s) 467, 868
Bsp143I GATC 6 cut(s) 73, 295, 322, 326, 340, 1183
Bsp68I TCGCGA 1 cut(s) 461
BspACI CCGC 5 cut(s) 54, 128, 135, 287, 311
BspANI GGCC 4 cut(s) 69, 338, 443, 1105
BspCNI CTCAG 5 cut(s) 676, 715, 940, 1018, 1051
BspDI ATCGAT 2 cut(s) 298, 325
BspFNI CGCG 3 cut(s) 202, 247, 461
BspLI GGNNCC 1 cut(s) 1209
BspPI GGATC 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 1207
BspTNI GGTCTC 2 cut(s) 467, 868
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 334
BsrI ACTGG 2 cut(s) 959, 1106
BssAI RCCGGY 1 cut(s) 334
BssMI GATC 6 cut(s) 73, 295, 322, 326, 340, 1183
BssNI GRCGYC 4 cut(s) 87, 123, 192, 261
Bst2UI CCWGG 2 cut(s) 478, 1205
Bst4CI ACNGT 2 cut(s) 821, 1045
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 4 cut(s) 87, 123, 192, 261
BstC8I GCNNGC 3 cut(s) 12, 336, 549
BstDEI CTNAG 5 cut(s) 684, 702, 948, 1026, 1059
BstENI CCTNNNNNAGG 1 cut(s) 1018
BstF5I GGATG 7 cut(s) 178, 466, 685, 735, 1052, 1117, 1198
BstFNI CGCG 3 cut(s) 202, 247, 461
BstHHI GCGC 4 cut(s) 249, 280, 416, 574
BstKTI GATC 6 cut(s) 76, 298, 325, 329, 343, 1186
BstMAI GTCTC 3 cut(s) 467, 868, 938
BstMBI GATC 6 cut(s) 73, 295, 322, 326, 340, 1183
BstMCI CGRYCG 2 cut(s) 325, 339
BstMWI GCNNNNNNNGC 6 cut(s) 54, 60, 286, 618, 664, 673
BstNI CCWGG 2 cut(s) 478, 1205
BstNSI RCATGY 1 cut(s) 551
BstSCI CCNGG 3 cut(s) 349, 476, 1203
BstUI CGCG 3 cut(s) 202, 247, 461
BstV1I GCAGC 1 cut(s) 571
BstV2I GAAGAC 5 cut(s) 195, 222, 384, 513, 1173
BstXI CCANNNNNNTGG 2 cut(s) 203, 477
BstZI CGGCCG 1 cut(s) 336
Bsu15I ATCGAT 2 cut(s) 298, 325
Bsu36I CCTNAGG 1 cut(s) 702
BsuI GTATCC 2 cut(s) 883, 918
BsuRI GGCC 4 cut(s) 69, 338, 443, 1105
BsuTUI ATCGAT 2 cut(s) 298, 325
BtgZI GCGATG 2 cut(s) 442, 476
BtsCI GGATG 7 cut(s) 178, 466, 685, 735, 1052, 1117, 1198
BtsIMutI CAGTG 1 cut(s) 426
BtuMI TCGCGA 1 cut(s) 461
Cac8I GCNNGC 3 cut(s) 12, 336, 549
CfoI GCGC 4 cut(s) 249, 280, 416, 574
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 1049
ClaI ATCGAT 2 cut(s) 298, 325
CseI GACGC 2 cut(s) 200, 250
CsiI ACCWGGT 1 cut(s) 476
Csp6I GTAC 1 cut(s) 92
CviAII CATG 5 cut(s) 6, 548, 782, 836, 925
CviQI GTAC 1 cut(s) 92
DdeI CTNAG 5 cut(s) 684, 702, 948, 1026, 1059
DpnI GATC 6 cut(s) 75, 297, 324, 328, 342, 1185
DpnII GATC 6 cut(s) 73, 295, 322, 326, 340, 1183
DraI TTTAAA 1 cut(s) 813
DrdI GACNNNNNNGTC 1 cut(s) 86
DriI GACNNNNNGTC 1 cut(s) 1047
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 2 cut(s) 336, 1103
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 381
Eam1105I GACNNNNNGTC 1 cut(s) 1047
EarI CTCTTC 1 cut(s) 381
EciI GGCGGA 1 cut(s) 300
EclXI CGGCCG 1 cut(s) 336
Eco31I GGTCTC 2 cut(s) 467, 868
Eco47I GGWCC 1 cut(s) 1049
Eco52I CGGCCG 1 cut(s) 336
Eco81I CCTNAGG 1 cut(s) 702
EcoNI CCTNNNNNAGG 1 cut(s) 1018
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 476, 1203
EcoT22I ATGCAT 1 cut(s) 468
FaeI CATG 5 cut(s) 9, 551, 785, 839, 928
FalI AAGNNNNNCTT 2 cut(s) 787, 819
FaqI GGGAC 1 cut(s) 110
FatI CATG 5 cut(s) 5, 547, 781, 835, 924
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 1 cut(s) 615
Fnu4HI GCNGC 2 cut(s) 55, 585
FokI GGATG 7 cut(s) 185, 453, 692, 742, 1039, 1124, 1205
Fsp4HI GCNGC 2 cut(s) 55, 585
FspBI CTAG 2 cut(s) 450, 1067
GlaI GCGC 4 cut(s) 248, 279, 415, 573
GluI GCNGC 2 cut(s) 55, 585
GsaI CCCAGC 1 cut(s) 541
HaeIII GGCC 4 cut(s) 69, 338, 443, 1105
HapII CCGG 3 cut(s) 335, 351, 860
HgaI GACGC 2 cut(s) 200, 250
HhaI GCGC 4 cut(s) 249, 280, 416, 574
Hin1I GRCGYC 4 cut(s) 87, 123, 192, 261
Hin1II CATG 5 cut(s) 9, 551, 785, 839, 928
Hin6I GCGC 4 cut(s) 247, 278, 414, 572
HinP1I GCGC 4 cut(s) 247, 278, 414, 572
HinfI GANTC 3 cut(s) 594, 751, 917
HpaII CCGG 3 cut(s) 335, 351, 860
HphI GGTGA 2 cut(s) 335, 1130
Hpy166II GTNNAC 1 cut(s) 696
Hpy188I TCNGA 8 cut(s) 172, 226, 295, 331, 532, 750, 778, 1060
Hpy188III TCNNGA 6 cut(s) 77, 460, 470, 494, 634, 1187
Hpy8I GTNNAC 1 cut(s) 696
HpyAV CCTTC 4 cut(s) 59, 454, 939, 1024
HpyCH4III ACNGT 2 cut(s) 821, 1045
HpyCH4IV ACGT 3 cut(s) 87, 123, 905
HpyCH4V TGCA 5 cut(s) 176, 466, 658, 733, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 60, 286, 618, 664, 673
HpyF3I CTNAG 5 cut(s) 684, 702, 948, 1026, 1059
HpySE526I ACGT 3 cut(s) 87, 123, 905
Hsp92I GRCGYC 4 cut(s) 87, 123, 192, 261
Hsp92II CATG 5 cut(s) 9, 551, 785, 839, 928
HspAI GCGC 4 cut(s) 247, 278, 414, 572
KroI GCCGGC 1 cut(s) 334
KroNI GCCGGC 1 cut(s) 336
Kzo9I GATC 6 cut(s) 73, 295, 322, 326, 340, 1183
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 7 cut(s) 163, 453, 475, 523, 670, 720, 966
MabI ACCWGGT 1 cut(s) 476
MaeI CTAG 2 cut(s) 450, 1067
MaeII ACGT 3 cut(s) 87, 123, 905
MaeIII GTNAC 3 cut(s) 424, 626, 1039
MalI GATC 6 cut(s) 75, 297, 324, 328, 342, 1185
MboI GATC 6 cut(s) 73, 295, 322, 326, 340, 1183
MlsI TGGCCA 1 cut(s) 1105
MluCI AATT 5 cut(s) 227, 251, 762, 1121, 1217
MluI ACGCGT 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 1105
MlyI GAGTC 1 cut(s) 745
MmeI TCCRAC 3 cut(s) 204, 462, 779
Mox20I TGGCCA 1 cut(s) 1105
Mph1103I ATGCAT 1 cut(s) 468
MroNI GCCGGC 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 802
MscI TGGCCA 1 cut(s) 1105
MseI TTAA 3 cut(s) 812, 1071, 1220
MslI CAYNNNNRTG 1 cut(s) 461
Msp20I TGGCCA 1 cut(s) 1105
MspA1I CMGCKG 1 cut(s) 1145
MspI CCGG 3 cut(s) 335, 351, 860
MspR9I CCNGG 3 cut(s) 351, 478, 1205
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 2 cut(s) 478, 1205
MvnI CGCG 3 cut(s) 202, 247, 461
MwoI GCNNNNNNNGC 6 cut(s) 54, 60, 286, 618, 664, 673
NaeI GCCGGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 351
NdeI CATATG 1 cut(s) 615
NdeII GATC 6 cut(s) 73, 295, 322, 326, 340, 1183
NgoMIV GCCGGC 1 cut(s) 334
NlaIII CATG 5 cut(s) 9, 551, 785, 839, 928
NlaIV GGNNCC 1 cut(s) 1209
NmeAIII GCCGAG 1 cut(s) 600
NmuCI GTSAC 2 cut(s) 424, 1039
NruI TCGCGA 1 cut(s) 461
NsiI ATGCAT 1 cut(s) 468
NspI RCATGY 1 cut(s) 551
PaeI GCATGC 1 cut(s) 551
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 87, 365, 1089
PctI GAATGC 1 cut(s) 658
PdiI GCCGGC 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 802
PfeI GAWTC 2 cut(s) 594, 917
PflFI GACNNNGTC 2 cut(s) 86, 434
PkrI GCNGC 2 cut(s) 56, 586
Ple19I CGATCG 1 cut(s) 325
PleI GAGTC 1 cut(s) 745
PpsI GAGTC 1 cut(s) 745
Psp6I CCWGG 2 cut(s) 476, 1203
PspFI CCCAGC 1 cut(s) 537
PspGI CCWGG 2 cut(s) 476, 1203
PspN4I GGNNCC 1 cut(s) 1209
PspPI GGNCC 1 cut(s) 1049
PsyI GACNNNGTC 2 cut(s) 86, 434
PvuI CGATCG 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 1145
RruI TCGCGA 1 cut(s) 461
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
RseI CAYNNNNRTG 1 cut(s) 461
SaqAI TTAA 3 cut(s) 812, 1071, 1220
SatI GCNGC 2 cut(s) 55, 585
Sau3AI GATC 6 cut(s) 73, 295, 322, 326, 340, 1183
Sau96I GGNCC 1 cut(s) 1049
SchI GAGTC 1 cut(s) 745
ScrFI CCNGG 3 cut(s) 351, 478, 1205
SexAI ACCWGGT 1 cut(s) 476
SfaNI GCATC 7 cut(s) 163, 453, 475, 523, 670, 720, 966
SinI GGWCC 1 cut(s) 1049
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 1187
SmoI CTYRAG 1 cut(s) 1187
SpeI ACTAGT 1 cut(s) 1066
SphI GCATGC 1 cut(s) 551
Sse9I AATT 5 cut(s) 227, 251, 762, 1121, 1217
SsiI CCGC 5 cut(s) 54, 128, 135, 287, 311
SspMI CTAG 2 cut(s) 450, 1067
StyD4I CCNGG 3 cut(s) 349, 476, 1203
TaaI ACNGT 2 cut(s) 821, 1045
TaiI ACGT 3 cut(s) 90, 126, 908
TaqI TCGA 6 cut(s) 42, 298, 325, 597, 662, 1002
TaqII GACCGA 1 cut(s) 1037
TasI AATT 5 cut(s) 227, 251, 762, 1121, 1217
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 2 cut(s) 594, 917
Tru1I TTAA 3 cut(s) 812, 1071, 1220
Tru9I TTAA 3 cut(s) 812, 1071, 1220
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 2 cut(s) 424, 1039
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 2 cut(s) 424, 1039
TspGWI ACGGA 3 cut(s) 26, 597, 985
TspRI CASTG 1 cut(s) 433
Tth111I GACNNNGTC 2 cut(s) 86, 434
VpaK11BI GGWCC 1 cut(s) 1049
XagI CCTNNNNNAGG 1 cut(s) 1018
XapI RAATTY 1 cut(s) 227
XceI RCATGY 1 cut(s) 551
XmnI GAANNNNTTC 1 cut(s) 802
XspI CTAG 2 cut(s) 450, 1067
ZraI GACGTC 2 cut(s) 88, 124
Zsp2I ATGCAT 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.