Prupe.1G420400_v2.0.a1

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0010000
Physical Location & Seq
Reverse (-)
36355302 .. 36358214
2913 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G420400.1

Sequence Viewer

Length: 1203 bp
ATGTCATGGCTCGCACGCTCTTTCGCTGACTCTCTACGGCTCGACGACGACGGTGGTGCCGAGAACGACGTCGTACCGAACAACAATGACAAAGCACCAATCTCACCCACCACCGACACCAAACCCCAAGAAGATGACAATGGAAGAAGTGGAATTGAATCTGCAGACGAGGAACAAGACGACGCCCAAACCAGAGGCGTGAAGGATGACCTCGACGAATTCAAACAAACCCTGACCCGCCAATTCTGGGGCGTCGCTACCTTCCTCGCGCCACCGCCTTCGGACTCCTCCACGCTGTCCACTCGATCAGGTCCCGATTTCGATCGATCTGAGCCGCCTGATAATCGGCCCGAGCAATTGGCGATGGATTTGATTGAAGACGAAGAGGATGAATTGGAAGAGTGCGCTGTTGGGGTCACTGAGGAGGTCCTGGCATTCGCTAAAAACATCGCGATGCATCCAGAAACGTGGCTGGACTTTCCTCTTGATGAGGAAGAGGACTTAGGCGATTTTGAAATGTCTGATGCCCAGCAAGAGCATGCTCTGGCGATTGAACATTTTGCTCCAAGATTAGCTGCTTTGAGATTTGAACTCTGTCCATGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTGCTTCTGCATTCGAGGCTCAATAAGCAGGATGCGGAGATTTTGTCCACACCCCAAGTAATGGAAGCCAGAGCAAAGTGGATGCAGGAGCTACAATGTCAGACTATGTCAGAATACGAAGGATTTGGAAGAAACACTTCTTTTCTAAAAGACAATTCTAACATACCAAATGATGATTTTGTTCCGGTCTCATCCTGTAGTGTTTATTATGAGGACAAGCTGCTAAGGACACATCCTTATGAATCAACCATGTCCCCCACAGTCCATGAGACTGAGAAGCATCCAGTTGAGAGTAATGAGATGCCATTCATTGATAAGTCTGTTATTGAAGAAAATCCCATCATTAAGATAGAGGATAAGAATTTAACAGTTGGTCCATCCTCTAAGATACTAGACAATATCTATGAAGATGATGAGGATGACTGGCCAGAGGAAGATTCTGATTTAGGCGGATATAGTGGAACGGCTATTCATGCGGTAAATGAAGAAGACATATCTTTCAGTGATCTCGAGGACGATGATTTCCTTACACCCACAAAACTCAAGATTGTCTCAAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

401

Amino Acids

45.31

Weight (kDa)

4.17

Isoelectric Point (pI)

67.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 56
AccB7I CCANNNNNTGG 1 cut(s) 700
AccII CGCG 2 cut(s) 269, 452
AciI CCGC 6 cut(s) 238, 275, 335, 674, 1089, 1115
AcoI YGGCCR 1 cut(s) 1064
AcsI RAATTY 2 cut(s) 218, 1000
AcyI GRCGYC 3 cut(s) 69, 183, 252
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 2 cut(s) 247, 700
AgsI TTSAA 7 cut(s) 158, 223, 377, 515, 554, 590, 968
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 3 cut(s) 575, 730, 859
AluI AGCT 3 cut(s) 575, 730, 859
Alw26I GTCTC 3 cut(s) 832, 902, 1195
Ama87I CYCGRG 2 cut(s) 350, 1148
AoxI GGCC 2 cut(s) 347, 1064
ApeKI GCWGC 2 cut(s) 575, 859
ApoI RAATTY 2 cut(s) 218, 1000
Asp700I GAANNNNTTC 1 cut(s) 775
AspLEI GCGC 2 cut(s) 271, 407
AspS9I GGNCC 4 cut(s) 311, 348, 427, 1013
AsuHPI GGTGA 1 cut(s) 96
AvaI CYCGRG 2 cut(s) 350, 1148
AvaII GGWCC 3 cut(s) 311, 427, 1013
BalI TGGCCA 1 cut(s) 1066
BanI GGYRCC 1 cut(s) 56
BbsI GAAGAC 2 cut(s) 384, 1134
BbvI GCAGC 2 cut(s) 562, 846
BccI CCATC 3 cut(s) 358, 986, 1024
BceAI ACGGC 2 cut(s) 53, 1119
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 1 cut(s) 431
BcoDI GTCTC 3 cut(s) 832, 902, 1195
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 2 cut(s) 162, 835
BisI GCNGC 3 cut(s) 335, 576, 860
BlsI GCNGC 3 cut(s) 336, 577, 861
Bme1390I CCNGG 1 cut(s) 431
Bme18I GGWCC 3 cut(s) 311, 427, 1013
BmeT110I CYCGRG 2 cut(s) 350, 1148
BmgT120I GGNCC 4 cut(s) 311, 348, 427, 1013
BmiI GGNNCC 2 cut(s) 58, 313
BmrFI CCNGG 1 cut(s) 431
BmsI GCATC 7 cut(s) 444, 466, 514, 661, 711, 928, 930
BpiI GAAGAC 2 cut(s) 384, 1134
Bpu10I CCTNAGC 1 cut(s) 863
BpuEI CTTGAG 1 cut(s) 1166
Bsa29I ATCGAT 1 cut(s) 325
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 3 cut(s) 69, 183, 252
BsaI GGTCTC 1 cut(s) 832
BsaWI WCCGGW 1 cut(s) 823
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 700
Bse1I ACTGG 2 cut(s) 923, 1067
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 1 cut(s) 431
BseCI ATCGAT 1 cut(s) 325
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 247, 700
BseMII CTCAG 3 cut(s) 321, 411, 903
BseNI ACTGG 2 cut(s) 923, 1067
BseRI GAGGAG 2 cut(s) 277, 437
BseXI GCAGC 2 cut(s) 562, 846
BseYI CCCAGC 1 cut(s) 528
Bsh1236I CGCG 2 cut(s) 269, 452
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 2 cut(s) 349, 1066
BshNI GGYRCC 1 cut(s) 56
BshVI ATCGAT 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 325
BsiHKCI CYCGRG 2 cut(s) 350, 1148
BsiSI CCGG 1 cut(s) 824
BslFI GGGAC 2 cut(s) 297, 877
BslI CCNNNNNNNGG 2 cut(s) 247, 700
BsmAI GTCTC 3 cut(s) 832, 902, 1195
BsmFI GGGAC 2 cut(s) 297, 877
BsmI GAATGC 2 cut(s) 434, 649
BsnI GGCC 2 cut(s) 349, 1066
Bso31I GGTCTC 1 cut(s) 832
BsoBI CYCGRG 2 cut(s) 350, 1148
Bsp143I GATC 4 cut(s) 305, 322, 326, 1144
Bsp68I TCGCGA 1 cut(s) 452
BspACI CCGC 6 cut(s) 238, 275, 335, 674, 1089, 1115
BspANI GGCC 2 cut(s) 349, 1066
BspCNI CTCAG 3 cut(s) 322, 412, 904
BspDI ATCGAT 1 cut(s) 325
BspFNI CGCG 2 cut(s) 269, 452
BspLI GGNNCC 2 cut(s) 58, 313
BspMAI CTGCAG 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 832
BsrI ACTGG 2 cut(s) 923, 1067
BssMI GATC 4 cut(s) 305, 322, 326, 1144
BssNI GRCGYC 3 cut(s) 69, 183, 252
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 3 cut(s) 53, 901, 1009
Bst6I CTCTTC 3 cut(s) 378, 393, 489
BstACI GRCGYC 3 cut(s) 69, 183, 252
BstC8I GCNNGC 3 cut(s) 12, 16, 540
BstDEI CTNAG 6 cut(s) 330, 420, 502, 863, 912, 1023
BstFNI CGCG 2 cut(s) 269, 452
BstHHI GCGC 2 cut(s) 271, 407
BstKTI GATC 4 cut(s) 308, 325, 329, 1147
BstMAI GTCTC 3 cut(s) 832, 902, 1195
BstMBI GATC 4 cut(s) 305, 322, 326, 1144
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 4 cut(s) 609, 655, 664, 1112
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 429
BstSFI CTRYAG 2 cut(s) 162, 835
BstUI CGCG 2 cut(s) 269, 452
BstV1I GCAGC 2 cut(s) 562, 846
BstV2I GAAGAC 2 cut(s) 384, 1134
BstXI CCANNNNNNTGG 1 cut(s) 468
Bsu15I ATCGAT 1 cut(s) 325
BsuRI GGCC 2 cut(s) 349, 1066
BsuTUI ATCGAT 1 cut(s) 325
BtgZI GCGATG 3 cut(s) 377, 433, 467
BtsIMutI CAGTG 2 cut(s) 417, 1147
BtuMI TCGCGA 1 cut(s) 452
Cac8I GCNNGC 3 cut(s) 12, 16, 540
CfoI GCGC 2 cut(s) 271, 407
Cfr13I GGNCC 4 cut(s) 311, 348, 427, 1013
ClaI ATCGAT 1 cut(s) 325
CseI GACGC 2 cut(s) 191, 241
Csp6I GTAC 1 cut(s) 74
CviAII CATG 6 cut(s) 6, 539, 600, 889, 905, 1112
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 6 cut(s) 330, 420, 502, 863, 912, 1023
DpnI GATC 4 cut(s) 307, 324, 328, 1146
DpnII GATC 4 cut(s) 305, 322, 326, 1144
EaeI YGGCCR 1 cut(s) 1064
Eam1104I CTCTTC 3 cut(s) 378, 393, 489
EarI CTCTTC 3 cut(s) 378, 393, 489
EciI GGCGGA 1 cut(s) 1104
Eco31I GGTCTC 1 cut(s) 832
Eco47I GGWCC 3 cut(s) 311, 427, 1013
Eco88I CYCGRG 2 cut(s) 350, 1148
EcoO109I RGGNCCY 2 cut(s) 311, 427
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 429
EcoT22I ATGCAT 1 cut(s) 459
FaeI CATG 6 cut(s) 9, 542, 603, 892, 908, 1115
FalI AAGNNNNNCTT 2 cut(s) 760, 792
FaqI GGGAC 2 cut(s) 297, 877
FatI CATG 6 cut(s) 5, 538, 599, 888, 904, 1111
FauI CCCGC 1 cut(s) 245
FauNDI CATATG 1 cut(s) 606
Fnu4HI GCNGC 3 cut(s) 335, 576, 860
Fsp4HI GCNGC 3 cut(s) 335, 576, 860
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 2 cut(s) 270, 406
GluI GCNGC 3 cut(s) 335, 576, 860
GsaI CCCAGC 1 cut(s) 532
HaeIII GGCC 2 cut(s) 349, 1066
HapII CCGG 1 cut(s) 824
HgaI GACGC 2 cut(s) 191, 241
HhaI GCGC 2 cut(s) 271, 407
Hin1I GRCGYC 3 cut(s) 69, 183, 252
Hin1II CATG 6 cut(s) 9, 542, 603, 892, 908, 1115
Hin6I GCGC 2 cut(s) 269, 405
HinP1I GCGC 2 cut(s) 269, 405
HinfI GANTC 5 cut(s) 29, 158, 284, 881, 1076
HpaII CCGG 1 cut(s) 824
HphI GGTGA 1 cut(s) 96
Hpy166II GTNNAC 2 cut(s) 300, 687
Hpy188I TCNGA 6 cut(s) 283, 331, 523, 741, 751, 1081
Hpy188III TCNNGA 7 cut(s) 314, 451, 461, 485, 625, 1148, 1183
Hpy8I GTNNAC 2 cut(s) 300, 687
Hpy99I CGWCG 8 cut(s) 47, 50, 53, 71, 74, 185, 218, 257
HpyAV CCTTC 4 cut(s) 196, 271, 288, 752
HpyCH4III ACNGT 3 cut(s) 53, 901, 1009
HpyCH4IV ACGT 2 cut(s) 69, 467
HpyCH4V TGCA 4 cut(s) 164, 457, 649, 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 609, 655, 664, 1112
HpyF3I CTNAG 6 cut(s) 330, 420, 502, 863, 912, 1023
HpySE526I ACGT 2 cut(s) 69, 467
Hsp92I GRCGYC 3 cut(s) 69, 183, 252
Hsp92II CATG 6 cut(s) 9, 542, 603, 892, 908, 1115
HspAI GCGC 2 cut(s) 269, 405
Kzo9I GATC 4 cut(s) 305, 322, 326, 1144
LmnI GCTCC 2 cut(s) 568, 727
Lsp1109I GCAGC 2 cut(s) 562, 846
LweI GCATC 7 cut(s) 444, 466, 514, 661, 711, 928, 930
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 2 cut(s) 69, 467
MaeIII GTNAC 2 cut(s) 415, 617
MalI GATC 4 cut(s) 307, 324, 328, 1146
MboI GATC 4 cut(s) 305, 322, 326, 1144
MfeI CAATTG 1 cut(s) 356
MlsI TGGCCA 1 cut(s) 1066
MluCI AATT 7 cut(s) 153, 218, 242, 356, 392, 793, 1000
MluNI TGGCCA 1 cut(s) 1066
MlyI GAGTC 2 cut(s) 23, 278
Mox20I TGGCCA 1 cut(s) 1066
Mph1103I ATGCAT 1 cut(s) 459
MroXI GAANNNNTTC 1 cut(s) 775
MscI TGGCCA 1 cut(s) 1066
MseI TTAA 2 cut(s) 984, 1004
MslI CAYNNNNRTG 2 cut(s) 452, 876
Msp20I TGGCCA 1 cut(s) 1066
MspI CCGG 1 cut(s) 824
MspR9I CCNGG 1 cut(s) 431
MunI CAATTG 1 cut(s) 356
Mva1269I GAATGC 2 cut(s) 434, 649
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 2 cut(s) 269, 452
MwoI GCNNNNNNNGC 4 cut(s) 609, 655, 664, 1112
NdeI CATATG 1 cut(s) 606
NdeII GATC 4 cut(s) 305, 322, 326, 1144
NlaIII CATG 6 cut(s) 9, 542, 603, 892, 908, 1115
NlaIV GGNNCC 2 cut(s) 58, 313
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 415
NruI TCGCGA 1 cut(s) 452
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 1 cut(s) 542
PaeI GCATGC 1 cut(s) 542
PaeR7I CTCGAG 1 cut(s) 1148
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 2 cut(s) 434, 649
PdmI GAANNNNTTC 1 cut(s) 775
PfeI GAWTC 3 cut(s) 158, 881, 1076
PflFI GACNNNGTC 1 cut(s) 745
PflMI CCANNNNNTGG 1 cut(s) 700
PkrI GCNGC 3 cut(s) 336, 577, 861
Ple19I CGATCG 1 cut(s) 325
PleI GAGTC 2 cut(s) 23, 278
PpsI GAGTC 2 cut(s) 23, 278
PpuMI RGGWCCY 2 cut(s) 311, 427
Psp5II RGGWCCY 2 cut(s) 311, 427
Psp6I CCWGG 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 528
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 2 cut(s) 58, 313
PspPI GGNCC 4 cut(s) 311, 348, 427, 1013
PspPPI RGGWCCY 2 cut(s) 311, 427
PstI CTGCAG 1 cut(s) 166
PsyI GACNNNGTC 1 cut(s) 745
PvuI CGATCG 1 cut(s) 325
RruI TCGCGA 1 cut(s) 452
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
RseI CAYNNNNRTG 2 cut(s) 452, 876
SaqAI TTAA 2 cut(s) 984, 1004
SatI GCNGC 3 cut(s) 335, 576, 860
Sau3AI GATC 4 cut(s) 305, 322, 326, 1144
Sau96I GGNCC 4 cut(s) 311, 348, 427, 1013
SchI GAGTC 2 cut(s) 23, 278
ScrFI CCNGG 1 cut(s) 431
SetI ASST 9 cut(s) 72, 213, 263, 313, 429, 470, 577, 732, 861
SfaNI GCATC 7 cut(s) 444, 466, 514, 661, 711, 928, 930
SfcI CTRYAG 2 cut(s) 162, 835
Sfr274I CTCGAG 1 cut(s) 1148
SinI GGWCC 3 cut(s) 311, 427, 1013
SlaI CTCGAG 1 cut(s) 1148
SmiMI CAYNNNNRTG 2 cut(s) 452, 876
SmlI CTYRAG 2 cut(s) 1148, 1181
SmoI CTYRAG 2 cut(s) 1148, 1181
SphI GCATGC 1 cut(s) 542
Sse9I AATT 7 cut(s) 153, 218, 242, 356, 392, 793, 1000
SsiI CCGC 6 cut(s) 238, 275, 335, 674, 1089, 1115
SspMI CTAG 1 cut(s) 1031
StyD4I CCNGG 1 cut(s) 429
TaaI ACNGT 3 cut(s) 53, 901, 1009
TaiI ACGT 2 cut(s) 72, 470
TaqI TCGA 7 cut(s) 42, 213, 304, 321, 325, 653, 1149
TasI AATT 7 cut(s) 153, 218, 242, 356, 392, 793, 1000
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 3 cut(s) 158, 881, 1076
Tru1I TTAA 2 cut(s) 984, 1004
Tru9I TTAA 2 cut(s) 984, 1004
TscAI CASTG 2 cut(s) 424, 1147
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 2 cut(s) 575, 859
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 6 cut(s) 405, 894, 937, 1059, 1100, 1137
TspRI CASTG 2 cut(s) 424, 1147
Tth111I GACNNNGTC 1 cut(s) 745
Van91I CCANNNNNTGG 1 cut(s) 700
VpaK11BI GGWCC 3 cut(s) 311, 427, 1013
XapI RAATTY 2 cut(s) 218, 1000
XceI RCATGY 1 cut(s) 542
XhoI CTCGAG 1 cut(s) 1148
XmnI GAANNNNTTC 1 cut(s) 775
XspI CTAG 1 cut(s) 1031
ZraI GACGTC 1 cut(s) 70
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.