Rh6DG490100

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010000
Physical Location & Seq
Forward (+)
65025340 .. 65028089
2750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG490100.1

Sequence Viewer

Length: 1230 bp
ATGTCATGGCTCGCCCACTCGCTCTCTAAATCCCTCCGTATCGACGGCGACGGCGAAGGCCAAGATCACGACGACGACGTCGTACCCCACCGCAACACCAACAATGAAGCTGACGTCCCGCCCACCGCCTCCACCAAACCCCAAGACAACAACGAAATCCAATCGGATGCAGAAGCAGTTGAAGACGCCCAAACGCGTGGCGTGAAAGAAGACCTATCGGAATTCCAGCAAACCCTAACGCGCCAATTCTGGGGCGTCGCCTCGTTTCTTGCGCCACCACCGCCCTCTGATCGATTAGCTTCTCCGCCCGATTGCGATCGATCCGCGCCGGCCGATCACCCACCCGGTCAATCGCCGATGGCGTTCAATGAAGGTGAAGAAGAAGAGGAGGAGGAGGAGTGCGCCGTCGGTGTCACTGACGAGGTCTTGGCCTTCGCTAGAAACATCGCGATGCATCCAGAGACCTGGTTGGACTTTCCTCTTGATGAAGAAGAAGACTTGGGAGATTTTGAAATGTCTGATGCCCAGCAAGAGCATGCTATGATGATTGAACATCTTGCGCCGAGATTAGCTGCTTTGAGAATCGAGCTTTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAACAAGCAGGATGCTGAGGTTTTGTCCACACCTCAGGTAATGGAAGCCAGAGCAATGTGGATGCAAGAGCTACAATGTCAGACTCCAACATCAATTTCTAAAATGTCAGAACATGAATGGTTTGGAAGAAGCACTTCACGTTTAAAATACAGTTCAAATGCAATACATGAAGATTATGAAGATTACTCTCCGGTCTCATCACATACTCCTCATTATGAGGATACACTTTATAAGACGTATCCTTTTGAATCAACCATGCCTTCCACAGCCGATGAGACTGAGAAGCACCCAGTTGAGAGTAATGAGATGCCATTCATTGATAAGTCCGTTATCGAAGAAAAGCCCTTCATTAGGACTGAGGATAAGAATGTGACAGTTGGTCCATCCTCTGAGGTACTAGTTAATAGCTATGACGATGACGACGACGATGACTGGCCAGAGGATGATGGTGAATTATATGGGTGTAGTGGAACAGCTGTTGCTTTGATAAATGAAGAAGACATATCTTTCAGTGATCTTGAGGATGATGATTTCCAGGCACCCTCTAAATTAAAGTTCATCTCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

409

Amino Acids

46.26

Weight (kDa)

4.23

Isoelectric Point (pI)

66.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 168 - 223 1.7e-13 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 888
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 2 cut(s) 81, 117
AccB1I GGYRCC 1 cut(s) 1195
AccII CGCG 4 cut(s) 196, 241, 326, 449
AciI CCGC 6 cut(s) 91, 119, 126, 281, 305, 324
AclWI GGATC 1 cut(s) 315
AcoI YGGCCR 2 cut(s) 330, 1091
AcsI RAATTY 1 cut(s) 221
AcyI GRCGYC 4 cut(s) 78, 114, 186, 255
AfaI GTAC 2 cut(s) 84, 1053
AfiI CCNNNNNNNGG 2 cut(s) 250, 1008
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 6 cut(s) 182, 367, 512, 551, 813, 905
AhdI GACNNNNNGTC 1 cut(s) 1035
AhlI ACTAGT 1 cut(s) 1054
AjnI CCWGG 2 cut(s) 464, 1191
AjuI GAANNNNNNNTTGG 2 cut(s) 736, 768
AluBI AGCT 7 cut(s) 110, 299, 572, 589, 727, 1065, 1133
AluI AGCT 7 cut(s) 110, 299, 572, 589, 727, 1065, 1133
Alw26I GTCTC 3 cut(s) 455, 856, 926
AlwI GGATC 1 cut(s) 315
AoxI GGCC 4 cut(s) 58, 330, 429, 1091
ApeKI GCWGC 1 cut(s) 572
ApoI RAATTY 1 cut(s) 221
Asp700I GAANNNNTTC 1 cut(s) 790
AspLEI GCGC 5 cut(s) 243, 274, 328, 404, 562
AspS9I GGNCC 1 cut(s) 1037
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 3 cut(s) 329, 386, 1118
AvaII GGWCC 1 cut(s) 1037
AxyI CCTNAGG 1 cut(s) 690
BalI TGGCCA 1 cut(s) 1093
BanI GGYRCC 1 cut(s) 1195
BbsI GAAGAC 4 cut(s) 189, 216, 501, 1161
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 559
BccI CCATC 3 cut(s) 352, 1048, 1097
BceAI ACGGC 3 cut(s) 61, 67, 389
BciT130I CCWGG 2 cut(s) 466, 1193
BciVI GTATCC 2 cut(s) 871, 906
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 3 cut(s) 455, 856, 926
BcuI ACTAGT 1 cut(s) 1054
BfaI CTAG 2 cut(s) 438, 1055
BfuI GTATCC 2 cut(s) 871, 906
BisI GCNGC 1 cut(s) 573
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 3 cut(s) 345, 466, 1193
Bme18I GGWCC 1 cut(s) 1037
BmeRI GACNNNNNGTC 1 cut(s) 1035
BmgT120I GGNCC 1 cut(s) 1037
BmiI GGNNCC 1 cut(s) 1197
BmrFI CCNGG 3 cut(s) 345, 466, 1193
BmrI ACTGGG 1 cut(s) 941
BmsI GCATC 7 cut(s) 157, 441, 463, 511, 658, 708, 954
BmuI ACTGGG 1 cut(s) 941
BpiI GAAGAC 4 cut(s) 189, 216, 501, 1161
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 1196
BpuMI CCSGG 1 cut(s) 345
Bsa29I ATCGAT 2 cut(s) 292, 319
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 4 cut(s) 78, 114, 186, 255
BsaI GGTCTC 2 cut(s) 455, 856
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 1008
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 2 cut(s) 947, 1094
Bse21I CCTNAGG 1 cut(s) 690
Bse3DI GCAATG 1 cut(s) 717
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 2 cut(s) 466, 1193
BseCI ATCGAT 2 cut(s) 292, 319
BseGI GGATG 7 cut(s) 172, 454, 673, 723, 1040, 1105, 1186
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 250, 1008
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 5 cut(s) 663, 704, 927, 1005, 1038
BseNI ACTGG 2 cut(s) 947, 1094
BseRI GAGGAG 5 cut(s) 401, 404, 407, 410, 855
BseX3I CGGCCG 1 cut(s) 330
BseXI GCAGC 1 cut(s) 559
BseYI CCCAGC 1 cut(s) 525
Bsh1236I CGCG 4 cut(s) 196, 241, 326, 449
Bsh1285I CGRYCG 2 cut(s) 319, 333
BshFI GGCC 4 cut(s) 60, 332, 431, 1093
BshNI GGYRCC 1 cut(s) 1195
BshVI ATCGAT 2 cut(s) 292, 319
BsiEI CGRYCG 2 cut(s) 319, 333
BsiSI CCGG 3 cut(s) 329, 345, 848
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 250, 1008
BsmAI GTCTC 3 cut(s) 455, 856, 926
BsmFI GGGAC 1 cut(s) 101
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 4 cut(s) 60, 332, 431, 1093
Bso31I GGTCTC 2 cut(s) 455, 856
Bsp143I GATC 6 cut(s) 64, 289, 316, 320, 334, 1171
Bsp68I TCGCGA 1 cut(s) 449
BspACI CCGC 6 cut(s) 91, 119, 126, 281, 305, 324
BspANI GGCC 4 cut(s) 60, 332, 431, 1093
BspCNI CTCAG 5 cut(s) 664, 703, 928, 1006, 1039
BspDI ATCGAT 2 cut(s) 292, 319
BspFNI CGCG 4 cut(s) 196, 241, 326, 449
BspLI GGNNCC 1 cut(s) 1197
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 1195
BspTNI GGTCTC 2 cut(s) 455, 856
BsrDI GCAATG 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 328
BsrI ACTGG 2 cut(s) 947, 1094
BssAI RCCGGY 1 cut(s) 328
BssMI GATC 6 cut(s) 64, 289, 316, 320, 334, 1171
BssNI GRCGYC 4 cut(s) 78, 114, 186, 255
Bst2UI CCWGG 2 cut(s) 466, 1193
Bst4CI ACNGT 2 cut(s) 809, 1033
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 4 cut(s) 78, 114, 186, 255
BstC8I GCNNGC 3 cut(s) 12, 330, 537
BstDEI CTNAG 5 cut(s) 672, 690, 936, 1014, 1047
BstENI CCTNNNNNAGG 1 cut(s) 1006
BstF5I GGATG 7 cut(s) 172, 454, 673, 723, 1040, 1105, 1186
BstFNI CGCG 4 cut(s) 196, 241, 326, 449
BstHHI GCGC 5 cut(s) 243, 274, 328, 404, 562
BstKTI GATC 6 cut(s) 67, 292, 319, 323, 337, 1174
BstMAI GTCTC 3 cut(s) 455, 856, 926
BstMBI GATC 6 cut(s) 64, 289, 316, 320, 334, 1171
BstMCI CGRYCG 2 cut(s) 319, 333
BstMWI GCNNNNNNNGC 4 cut(s) 280, 606, 652, 661
BstNI CCWGG 2 cut(s) 466, 1193
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 3 cut(s) 343, 464, 1191
BstUI CGCG 4 cut(s) 196, 241, 326, 449
BstV1I GCAGC 1 cut(s) 559
BstV2I GAAGAC 4 cut(s) 189, 216, 501, 1161
BstXI CCANNNNNNTGG 2 cut(s) 197, 465
BstZI CGGCCG 1 cut(s) 330
Bsu15I ATCGAT 2 cut(s) 292, 319
Bsu36I CCTNAGG 1 cut(s) 690
BsuI GTATCC 2 cut(s) 871, 906
BsuRI GGCC 4 cut(s) 60, 332, 431, 1093
BsuTUI ATCGAT 2 cut(s) 292, 319
BtgZI GCGATG 2 cut(s) 430, 464
BtsCI GGATG 7 cut(s) 172, 454, 673, 723, 1040, 1105, 1186
BtsIMutI CAGTG 2 cut(s) 414, 1174
BtuMI TCGCGA 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 12, 330, 537
CfoI GCGC 5 cut(s) 243, 274, 328, 404, 562
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 1 cut(s) 1037
ClaI ATCGAT 2 cut(s) 292, 319
CseI GACGC 2 cut(s) 194, 244
CsiI ACCWGGT 1 cut(s) 464
Csp6I GTAC 2 cut(s) 83, 1052
CviAII CATG 5 cut(s) 6, 536, 770, 824, 913
CviQI GTAC 2 cut(s) 83, 1052
DdeI CTNAG 5 cut(s) 672, 690, 936, 1014, 1047
DpnI GATC 6 cut(s) 66, 291, 318, 322, 336, 1173
DpnII GATC 6 cut(s) 64, 289, 316, 320, 334, 1171
DraI TTTAAA 1 cut(s) 801
DrdI GACNNNNNNGTC 1 cut(s) 77
DriI GACNNNNNGTC 1 cut(s) 1035
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 2 cut(s) 330, 1091
EagI CGGCCG 1 cut(s) 330
Eam1104I CTCTTC 1 cut(s) 378
Eam1105I GACNNNNNGTC 1 cut(s) 1035
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 1 cut(s) 294
EclXI CGGCCG 1 cut(s) 330
Eco31I GGTCTC 2 cut(s) 455, 856
Eco47I GGWCC 1 cut(s) 1037
Eco52I CGGCCG 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 690
EcoNI CCTNNNNNAGG 1 cut(s) 1006
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 464, 1191
EcoT22I ATGCAT 1 cut(s) 456
FaeI CATG 5 cut(s) 9, 539, 773, 827, 916
FalI AAGNNNNNCTT 2 cut(s) 775, 807
FaqI GGGAC 1 cut(s) 101
FatI CATG 5 cut(s) 5, 535, 769, 823, 912
FauI CCCGC 1 cut(s) 126
FauNDI CATATG 1 cut(s) 603
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 7 cut(s) 179, 441, 680, 730, 1027, 1112, 1193
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 2 cut(s) 438, 1055
GlaI GCGC 5 cut(s) 242, 273, 327, 403, 561
GluI GCNGC 1 cut(s) 573
GsaI CCCAGC 1 cut(s) 529
HaeIII GGCC 4 cut(s) 60, 332, 431, 1093
HapII CCGG 3 cut(s) 329, 345, 848
HgaI GACGC 2 cut(s) 194, 244
HhaI GCGC 5 cut(s) 243, 274, 328, 404, 562
Hin1I GRCGYC 4 cut(s) 78, 114, 186, 255
Hin1II CATG 5 cut(s) 9, 539, 773, 827, 916
Hin6I GCGC 5 cut(s) 241, 272, 326, 402, 560
HinP1I GCGC 5 cut(s) 241, 272, 326, 402, 560
HinfI GANTC 3 cut(s) 582, 739, 905
HpaII CCGG 3 cut(s) 329, 345, 848
HphI GGTGA 3 cut(s) 329, 386, 1118
Hpy166II GTNNAC 1 cut(s) 684
Hpy188I TCNGA 7 cut(s) 166, 220, 289, 520, 738, 766, 1048
Hpy188III TCNNGA 6 cut(s) 68, 448, 458, 482, 622, 1175
Hpy8I GTNNAC 1 cut(s) 684
HpyAV CCTTC 5 cut(s) 50, 365, 442, 927, 1012
HpyCH4III ACNGT 2 cut(s) 809, 1033
HpyCH4IV ACGT 4 cut(s) 78, 114, 796, 893
HpyCH4V TGCA 5 cut(s) 170, 454, 646, 721, 818
HpyF10VI GCNNNNNNNGC 4 cut(s) 280, 606, 652, 661
HpyF3I CTNAG 5 cut(s) 672, 690, 936, 1014, 1047
HpySE526I ACGT 4 cut(s) 78, 114, 796, 893
Hsp92I GRCGYC 4 cut(s) 78, 114, 186, 255
Hsp92II CATG 5 cut(s) 9, 539, 773, 827, 916
HspAI GCGC 5 cut(s) 241, 272, 326, 402, 560
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Kzo9I GATC 6 cut(s) 64, 289, 316, 320, 334, 1171
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 7 cut(s) 157, 441, 463, 511, 658, 708, 954
MabI ACCWGGT 1 cut(s) 464
MaeI CTAG 2 cut(s) 438, 1055
MaeII ACGT 4 cut(s) 78, 114, 796, 893
MaeIII GTNAC 3 cut(s) 412, 614, 1027
MalI GATC 6 cut(s) 66, 291, 318, 322, 336, 1173
MboI GATC 6 cut(s) 64, 289, 316, 320, 334, 1171
MlsI TGGCCA 1 cut(s) 1093
MluCI AATT 5 cut(s) 221, 245, 750, 1109, 1205
MluI ACGCGT 1 cut(s) 194
MluNI TGGCCA 1 cut(s) 1093
MlyI GAGTC 1 cut(s) 733
MmeI TCCRAC 2 cut(s) 450, 767
Mox20I TGGCCA 1 cut(s) 1093
Mph1103I ATGCAT 1 cut(s) 456
MroNI GCCGGC 1 cut(s) 328
MroXI GAANNNNTTC 1 cut(s) 790
MscI TGGCCA 1 cut(s) 1093
MseI TTAA 3 cut(s) 800, 1059, 1208
MslI CAYNNNNRTG 1 cut(s) 449
Msp20I TGGCCA 1 cut(s) 1093
MspA1I CMGCKG 1 cut(s) 1133
MspI CCGG 3 cut(s) 329, 345, 848
MspR9I CCNGG 3 cut(s) 345, 466, 1193
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 2 cut(s) 466, 1193
MvnI CGCG 4 cut(s) 196, 241, 326, 449
MwoI GCNNNNNNNGC 4 cut(s) 280, 606, 652, 661
NaeI GCCGGC 1 cut(s) 330
NciI CCSGG 1 cut(s) 345
NdeI CATATG 1 cut(s) 603
NdeII GATC 6 cut(s) 64, 289, 316, 320, 334, 1171
NgoMIV GCCGGC 1 cut(s) 328
NlaIII CATG 5 cut(s) 9, 539, 773, 827, 916
NlaIV GGNNCC 1 cut(s) 1197
NmeAIII GCCGAG 1 cut(s) 588
NmuCI GTSAC 2 cut(s) 412, 1027
NruI TCGCGA 1 cut(s) 449
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 1 cut(s) 539
PaeI GCATGC 1 cut(s) 539
PcsI WCGNNNNNNNCGW 5 cut(s) 51, 75, 78, 359, 1077
PctI GAATGC 1 cut(s) 646
PdiI GCCGGC 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 790
PfeI GAWTC 2 cut(s) 582, 905
PflFI GACNNNGTC 2 cut(s) 77, 422
PkrI GCNGC 1 cut(s) 574
Ple19I CGATCG 1 cut(s) 319
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
PsiI TTATAA 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 464, 1191
PspFI CCCAGC 1 cut(s) 525
PspGI CCWGG 2 cut(s) 464, 1191
PspN4I GGNNCC 1 cut(s) 1197
PspPI GGNCC 1 cut(s) 1037
PsyI GACNNNGTC 2 cut(s) 77, 422
PvuI CGATCG 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 1133
RruI TCGCGA 1 cut(s) 449
RsaI GTAC 2 cut(s) 84, 1053
RsaNI GTAC 2 cut(s) 83, 1052
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 3 cut(s) 800, 1059, 1208
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 6 cut(s) 64, 289, 316, 320, 334, 1171
Sau96I GGNCC 1 cut(s) 1037
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 3 cut(s) 345, 466, 1193
SexAI ACCWGGT 1 cut(s) 464
SfaNI GCATC 7 cut(s) 157, 441, 463, 511, 658, 708, 954
SinI GGWCC 1 cut(s) 1037
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 1175
SmoI CTYRAG 1 cut(s) 1175
SpeI ACTAGT 1 cut(s) 1054
SphI GCATGC 1 cut(s) 539
Sse9I AATT 5 cut(s) 221, 245, 750, 1109, 1205
SsiI CCGC 6 cut(s) 91, 119, 126, 281, 305, 324
SspMI CTAG 2 cut(s) 438, 1055
StyD4I CCNGG 3 cut(s) 343, 464, 1191
TaaI ACNGT 2 cut(s) 809, 1033
TaiI ACGT 4 cut(s) 81, 117, 799, 896
TaqI TCGA 6 cut(s) 42, 292, 319, 585, 650, 990
TasI AATT 5 cut(s) 221, 245, 750, 1109, 1205
TfiI GAWTC 2 cut(s) 582, 905
Tru1I TTAA 3 cut(s) 800, 1059, 1208
Tru9I TTAA 3 cut(s) 800, 1059, 1208
TscAI CASTG 2 cut(s) 421, 1174
TseFI GTSAC 2 cut(s) 412, 1027
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 2 cut(s) 412, 1027
TspGWI ACGGA 3 cut(s) 26, 585, 973
TspRI CASTG 2 cut(s) 421, 1174
Tth111I GACNNNGTC 2 cut(s) 77, 422
VpaK11BI GGWCC 1 cut(s) 1037
XagI CCTNNNNNAGG 1 cut(s) 1006
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 790
XspI CTAG 2 cut(s) 438, 1055
ZraI GACGTC 2 cut(s) 79, 115
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.