pycom15g06320

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010000
Physical Location & Seq
Reverse (-)
3853563 .. 3855439
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g06320.2

Sequence Viewer

Length: 876 bp
ATGTCATGGCTCGCTCGCTCTCTCGCTGACTCTCTCCGGCTCGACGAGTTCGGCGACGCCGAGAACGACGTCGTACCCAACGAATCAGCAATCCCAACCACCGCCGACAACGACGTCGTTCGAAGAAGCGAAATTGAATCTTCAGATAAGGAGAACGAAGATGACGACCAGACCCGAGGCGTGAGGGAAGATTTGGACGAATTCAAGCAGACGCTGACCCGTCAATTCTGGGGGGTCGCATCATTCCTCGCTCCGCCGCCGCCTCCACCGCCTCCGCCGTACCCTTCATTGCCATCCAGTAATCGAACGGATCCTCCCGACATCGGTCGATCCGAACCGCCCGATCAATTGGCGATGGATTTTGGTGAAGGAGAAGAGGAGGAGGAGGAATTGGAGGAGGATTGCGCCGTAGGGGTCACTGAGGAGGTGCTGGCGTTCGCGAGGAACATCGCGATGCATCCGGAGACGTGGCTGGACTTTCCTCTCGACGAAGAGGAGGATTTGGGCGATTTTGACATGTCTGATGCCCAGCAAGAGCATGCTACGGTGATTGAACATCTTGCGCCAAGATTAGCTGCTTTGAGATTTGAACTCTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAATAAGCAGGACGCCGAGATCTTGTCCACACCCCAAGTAAGCTTCCCCCTTTGTATTATTACTATTAGTGTAATGGAAGCCAGAGCAATGTGGATGCAGGAGCTACATTGTTATACTGCATTAGAAGATGATCAATTTGGAAGAAACCCTTCTTTTTTAAAAGACCATTCCAATATACTACACGAAGATTGTGCTCCAGTCTCATCCTGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

292

Amino Acids

32.92

Weight (kDa)

4.18

Isoelectric Point (pI)

74.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AatII GACGTC 2 cut(s) 72, 117
AccII CGCG 2 cut(s) 440, 452
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 7 cut(s) 102, 254, 257, 260, 269, 275, 338
AclWI GGATC 3 cut(s) 305, 318, 324
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 4 cut(s) 57, 69, 114, 672
AfaI GTAC 2 cut(s) 75, 281
AfiI CCNNNNNNNGG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 516
AgsI TTSAA 4 cut(s) 137, 205, 554, 590
AjiI CACGTC 1 cut(s) 468
AloI GAACNNNNNNTCC 2 cut(s) 298, 330
AluBI AGCT 3 cut(s) 575, 702, 763
AluI AGCT 3 cut(s) 575, 702, 763
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 2 cut(s) 458, 865
AlwI GGATC 3 cut(s) 305, 318, 324
AlwNI CAGNNNCTG 1 cut(s) 214
Ama87I CYCGRG 1 cut(s) 174
Aor13HI TCCGGA 1 cut(s) 460
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 1 cut(s) 200
Asp700I GAANNNNTTC 1 cut(s) 808
AspLEI GCGC 2 cut(s) 407, 565
AsuHPI GGTGA 2 cut(s) 377, 559
AsuII TTCGAA 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 174
BamHI GGATCC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 856
BbvI GCAGC 1 cut(s) 562
BccI CCATC 2 cut(s) 301, 349
BceAI ACGGC 2 cut(s) 262, 392
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BclI TGATCA 1 cut(s) 790
BcoDI GTCTC 2 cut(s) 458, 865
BfmI CTRYAG 1 cut(s) 868
BglII AGATCT 1 cut(s) 678
BisI GCNGC 3 cut(s) 257, 260, 576
BlsI GCNGC 3 cut(s) 258, 261, 577
BmeT110I CYCGRG 1 cut(s) 174
BmgBI CACGTC 1 cut(s) 468
BmiI GGNNCC 1 cut(s) 312
BmsI GCATC 5 cut(s) 248, 444, 466, 514, 744
BoxI GACNNNNGTC 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 840
Bpu14I TTCGAA 1 cut(s) 121
BsaHI GRCGYC 4 cut(s) 57, 69, 114, 672
BsaJI CCNNGG 1 cut(s) 175
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 298, 328
Bsc4I CCNNNNNNNGG 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 297, 857
Bse3DI GCAATG 2 cut(s) 287, 753
BseAI TCCGGA 1 cut(s) 460
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 4 cut(s) 293, 457, 759, 863
BseLI CCNNNNNNNGG 1 cut(s) 323
BseMI GCAATG 2 cut(s) 287, 753
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 2 cut(s) 297, 857
BseRI GAGGAG 6 cut(s) 392, 395, 398, 410, 437, 509
BseXI GCAGC 1 cut(s) 562
BseYI CCCAGC 1 cut(s) 528
Bsh1236I CGCG 2 cut(s) 440, 452
Bsh1285I CGRYCG 1 cut(s) 328
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 856
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 2 cut(s) 37, 461
BslI CCNNNNNNNGG 1 cut(s) 323
BsmAI GTCTC 2 cut(s) 458, 865
BsmBI CGTCTC 1 cut(s) 458
BsmI GAATGC 1 cut(s) 649
BsoBI CYCGRG 1 cut(s) 174
Bsp119I TTCGAA 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 856
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 5 cut(s) 310, 329, 343, 678, 790
Bsp68I TCGCGA 2 cut(s) 440, 452
BspACI CCGC 7 cut(s) 102, 254, 257, 260, 269, 275, 338
BspCNI CTCAG 1 cut(s) 412
BspEI TCCGGA 1 cut(s) 460
BspFNI CGCG 2 cut(s) 440, 452
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 3 cut(s) 305, 318, 324
BspT104I TTCGAA 1 cut(s) 121
BsrDI GCAATG 2 cut(s) 287, 753
BsrI ACTGG 2 cut(s) 297, 857
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 5 cut(s) 310, 329, 343, 678, 790
BssNI GRCGYC 4 cut(s) 57, 69, 114, 672
Bst4CI ACNGT 1 cut(s) 547
Bst6I CTCTTC 2 cut(s) 369, 486
BstACI GRCGYC 4 cut(s) 57, 69, 114, 672
BstBI TTCGAA 1 cut(s) 121
BstC8I GCNNGC 4 cut(s) 12, 16, 432, 540
BstDEI CTNAG 1 cut(s) 420
BstF5I GGATG 4 cut(s) 293, 457, 759, 863
BstFNI CGCG 2 cut(s) 440, 452
BstHHI GCGC 2 cut(s) 407, 565
BstKTI GATC 5 cut(s) 313, 332, 346, 681, 793
BstMAI GTCTC 2 cut(s) 458, 865
BstMBI GATC 5 cut(s) 310, 329, 343, 678, 790
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 4 cut(s) 268, 609, 655, 664
BstNSI RCATGY 2 cut(s) 520, 542
BstPAI GACNNNNGTC 1 cut(s) 324
BstSFI CTRYAG 1 cut(s) 868
BstUI CGCG 2 cut(s) 440, 452
BstV1I GCAGC 1 cut(s) 562
BstX2I RGATCY 2 cut(s) 310, 678
BstYI RGATCY 2 cut(s) 310, 678
BtgZI GCGATG 3 cut(s) 368, 433, 467
BtrI CACGTC 1 cut(s) 468
BtsCI GGATG 4 cut(s) 293, 457, 759, 863
BtsIMutI CAGTG 1 cut(s) 417
BtuMI TCGCGA 2 cut(s) 440, 452
Cac8I GCNNGC 4 cut(s) 12, 16, 432, 540
CaiI CAGNNNCTG 1 cut(s) 214
CfoI GCGC 2 cut(s) 407, 565
CseI GACGC 3 cut(s) 65, 220, 680
Csp6I GTAC 2 cut(s) 74, 280
CviAII CATG 3 cut(s) 6, 517, 539
CviJI RGCY 8 cut(s) 10, 40, 472, 575, 658, 702, 740, 763
CviKI_1 RGCY 8 cut(s) 10, 40, 472, 575, 658, 702, 740, 763
CviQI GTAC 2 cut(s) 74, 280
DdeI CTNAG 1 cut(s) 420
DpnI GATC 5 cut(s) 312, 331, 345, 680, 792
DpnII GATC 5 cut(s) 310, 329, 343, 678, 790
DraI TTTAAA 1 cut(s) 819
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
Eam1104I CTCTTC 2 cut(s) 369, 486
EarI CTCTTC 2 cut(s) 369, 486
EciI GGCGGA 2 cut(s) 243, 264
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 174
EcoRI GAATTC 1 cut(s) 200
EcoT22I ATGCAT 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 3 cut(s) 9, 520, 542
FaiI YATR 7 cut(s) 7, 518, 540, 606, 608, 774, 836
FalI AAGNNNNNCTT 2 cut(s) 793, 825
FatI CATG 3 cut(s) 5, 516, 538
FauNDI CATATG 1 cut(s) 606
FbaI TGATCA 1 cut(s) 790
Fnu4HI GCNGC 3 cut(s) 257, 260, 576
FokI GGATG 4 cut(s) 280, 444, 766, 850
Fsp4HI GCNGC 3 cut(s) 257, 260, 576
GlaI GCGC 2 cut(s) 406, 564
GluI GCNGC 3 cut(s) 257, 260, 576
GsaI CCCAGC 1 cut(s) 532
GsuI CTGGAG 1 cut(s) 840
HapII CCGG 2 cut(s) 37, 461
HgaI GACGC 3 cut(s) 65, 220, 680
HhaI GCGC 2 cut(s) 407, 565
Hin1I GRCGYC 4 cut(s) 57, 69, 114, 672
Hin1II CATG 3 cut(s) 9, 520, 542
Hin6I GCGC 2 cut(s) 405, 563
HinP1I GCGC 2 cut(s) 405, 563
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 3 cut(s) 29, 83, 137
HpaII CCGG 2 cut(s) 37, 461
HphI GGTGA 2 cut(s) 377, 559
Hpy166II GTNNAC 1 cut(s) 687
Hpy188I TCNGA 3 cut(s) 145, 334, 523
Hpy188III TCNNGA 6 cut(s) 317, 439, 451, 461, 485, 625
Hpy8I GTNNAC 1 cut(s) 687
Hpy99I CGWCG 7 cut(s) 47, 59, 71, 74, 116, 119, 491
HpyAV CCTTC 3 cut(s) 294, 362, 819
HpyCH4III ACNGT 1 cut(s) 547
HpyCH4IV ACGT 3 cut(s) 69, 114, 467
HpyCH4V TGCA 4 cut(s) 457, 649, 757, 779
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 609, 655, 664
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 3 cut(s) 69, 114, 467
Hsp92I GRCGYC 4 cut(s) 57, 69, 114, 672
Hsp92II CATG 3 cut(s) 9, 520, 542
HspAI GCGC 2 cut(s) 405, 563
Kpn2I TCCGGA 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 790
Kzo9I GATC 5 cut(s) 310, 329, 343, 678, 790
LmnI GCTCC 3 cut(s) 256, 760, 859
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 5 cut(s) 248, 444, 466, 514, 744
MaeII ACGT 3 cut(s) 69, 114, 467
MaeIII GTNAC 2 cut(s) 415, 617
MalI GATC 5 cut(s) 312, 331, 345, 680, 792
MboI GATC 5 cut(s) 310, 329, 343, 678, 790
MfeI CAATTG 1 cut(s) 347
MflI RGATCY 2 cut(s) 310, 678
MhlI GDGCHC 1 cut(s) 856
MluCI AATT 6 cut(s) 132, 200, 224, 347, 389, 794
MlyI GAGTC 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 459
MroI TCCGGA 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 808
MseI TTAA 1 cut(s) 818
MslI CAYNNNNRTG 1 cut(s) 452
MspI CCGG 2 cut(s) 37, 461
MunI CAATTG 1 cut(s) 347
Mva1269I GAATGC 1 cut(s) 649
MvnI CGCG 2 cut(s) 440, 452
MwoI GCNNNNNNNGC 4 cut(s) 268, 609, 655, 664
NdeI CATATG 1 cut(s) 606
NdeII GATC 5 cut(s) 310, 329, 343, 678, 790
NlaIII CATG 3 cut(s) 9, 520, 542
NlaIV GGNNCC 1 cut(s) 312
NmeAIII GCCGAG 2 cut(s) 85, 700
NmuCI GTSAC 1 cut(s) 415
NruI TCGCGA 2 cut(s) 440, 452
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 2 cut(s) 520, 542
NspV TTCGAA 1 cut(s) 121
PaeI GCATGC 1 cut(s) 542
PciI ACATGT 1 cut(s) 516
PcsI WCGNNNNNNNCGW 6 cut(s) 51, 57, 63, 78, 162, 330
PctI GAATGC 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 808
PfeI GAWTC 2 cut(s) 83, 137
PkrI GCNGC 3 cut(s) 258, 261, 577
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 1 cut(s) 516
PshAI GACNNNNGTC 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 528
PspN4I GGNNCC 1 cut(s) 312
PstNI CAGNNNCTG 1 cut(s) 214
PsuI RGATCY 2 cut(s) 310, 678
RruI TCGCGA 2 cut(s) 440, 452
RsaI GTAC 2 cut(s) 75, 281
RsaNI GTAC 2 cut(s) 74, 280
RseI CAYNNNNRTG 1 cut(s) 452
SaqAI TTAA 1 cut(s) 818
SatI GCNGC 3 cut(s) 257, 260, 576
Sau3AI GATC 5 cut(s) 310, 329, 343, 678, 790
SchI GAGTC 1 cut(s) 23
SduI GDGCHC 1 cut(s) 856
SetI ASST 7 cut(s) 72, 117, 429, 470, 577, 704, 765
SfaNI GCATC 5 cut(s) 248, 444, 466, 514, 744
SfcI CTRYAG 1 cut(s) 868
SfuI TTCGAA 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 452
SphI GCATGC 1 cut(s) 542
Sse9I AATT 6 cut(s) 132, 200, 224, 347, 389, 794
SsiI CCGC 7 cut(s) 102, 254, 257, 260, 269, 275, 338
TaaI ACNGT 1 cut(s) 547
TaiI ACGT 3 cut(s) 72, 117, 470
TaqI TCGA 6 cut(s) 42, 121, 304, 328, 486, 653
TaqII GACCGA 1 cut(s) 314
TasI AATT 6 cut(s) 132, 200, 224, 347, 389, 794
TauI GCSGC 2 cut(s) 259, 262
TfiI GAWTC 2 cut(s) 83, 137
Tru1I TTAA 1 cut(s) 818
Tru9I TTAA 1 cut(s) 818
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 1 cut(s) 276
TspGWI ACGGA 2 cut(s) 323, 588
TspRI CASTG 1 cut(s) 424
XapI RAATTY 1 cut(s) 200
XceI RCATGY 2 cut(s) 520, 542
XmnI GAANNNNTTC 1 cut(s) 808
ZraI GACGTC 2 cut(s) 70, 115
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.