Rh6BG499000

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010000
Physical Location & Seq
Forward (+)
69934662 .. 69937351
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG499000.1

Sequence Viewer

Length: 1218 bp
ATGTCATGGCTCGCCCACTCGCTCTCTAAATCCCTCCGTATCGACGGCGACGGCGAAGGCCAAGATCACGACGACGACGACGACGTCGTACCCCACCGCAACACCAACAATGAAGCTGACGTCCCGCCCACCGCCTCCACCAAACCCCAAGACAAAGACAACAACGAAATCCAATCGGATGCAGAAGCAGTTGAAGACGCCCAAACGCGTGGCGTGAAAGAAGACCTATCGGAATTCCAGCAAACCCTAACGCGCCAATTCTGGGGCGTCGCCTCGTTCCTTGCGCCACCACCGCCCGATTGCGATCGATCCGAGCCGGCCGATCACCCACCCGGTCAATCGCCGATGGCGTTCAATGAAGGTGAAGAAGAGGAGGAGGAGGAGGAGTGCGCCGTCGGTGTCACTGACGAGGTCTTGGCCTTCGCTAGAAACATCGCGATGCATCCAGAGACCTGGTTGGACTTTCCTCTTGATGAAGAAGAAGACTTGGGAGATTTTGAAATGTCTGATGCCCAGCAAGAGCATGCTATGATGATTGAACATCTTGCGCCGAGATTAGCTGCTTTGAGAATCGAGCTTTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAACAAGCAGGATGCTGAGGTTTTGTCCACACCTCAGGTAATGGAAGCCAGAGCAATGTGGATGCAAGAGCTACAATGTCAGACTCCAACATCAATTTCTAAAATGTCAGAACATGAATGGTTTGGAAGAAGCACTTCACGTTTAAAATACAGTTCAAATGCAATCCATGAAGATTATGAAGATTACTCTCCGGTCTCATCACATACTCCTCATTATGAGGATACACTTCATAAGACGTATCCTTTTGAATCAACCATGCCTTCCACAGCCGATGAGACTGAGAAGCACCCAGTTGAGAGTAATGAGATGCCATTCATTGATAAGTCCGTTATCGAAGAAAAGCCCTTCATTAGGACTGAGGATAAGAATGTGACAGTTGGTCCGTCCTCTGAGATACTAGTTAATAGCTATGACGACGATGACAATGATGACTGGCCAGAGGATGATGGTGAATTATATGGGTGTAGTGGAACAGCTGTTGCTTTGATAAATGAAGAAGACATATCTTTCAGTGATCTTGAGGATGATGATTTCCAGGCACCCGCTAAATTAAGGTTCATCTCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

405

Amino Acids

45.96

Weight (kDa)

4.23

Isoelectric Point (pI)

68.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 164 - 219 1.7e-13 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AatII GACGTC 2 cut(s) 87, 123
AccB1I GGYRCC 1 cut(s) 1183
AccII CGCG 3 cut(s) 208, 253, 437
AciI CCGC 5 cut(s) 97, 125, 132, 293, 1188
AclWI GGATC 1 cut(s) 303
AcoI YGGCCR 2 cut(s) 318, 1079
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 4 cut(s) 84, 120, 198, 267
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 2 cut(s) 262, 996
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 6 cut(s) 194, 355, 500, 539, 801, 893
AhdI GACNNNNNGTC 1 cut(s) 1023
AhlI ACTAGT 1 cut(s) 1042
AjnI CCWGG 2 cut(s) 452, 1179
AjuI GAANNNNNNNTTGG 2 cut(s) 724, 756
AluBI AGCT 6 cut(s) 116, 560, 577, 715, 1053, 1121
AluI AGCT 6 cut(s) 116, 560, 577, 715, 1053, 1121
Alw26I GTCTC 3 cut(s) 443, 844, 914
AlwI GGATC 1 cut(s) 303
AoxI GGCC 4 cut(s) 58, 318, 417, 1079
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 1 cut(s) 233
Asp700I GAANNNNTTC 1 cut(s) 778
AspLEI GCGC 4 cut(s) 255, 286, 392, 550
AspS9I GGNCC 1 cut(s) 1025
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 3 cut(s) 317, 374, 1106
AvaII GGWCC 1 cut(s) 1025
AxyI CCTNAGG 1 cut(s) 678
BalI TGGCCA 1 cut(s) 1081
BanI GGYRCC 1 cut(s) 1183
BbsI GAAGAC 4 cut(s) 201, 228, 489, 1149
BbvCI CCTCAGC 1 cut(s) 660
BbvI GCAGC 1 cut(s) 547
BccI CCATC 2 cut(s) 340, 1085
BceAI ACGGC 3 cut(s) 61, 67, 377
BciT130I CCWGG 2 cut(s) 454, 1181
BciVI GTATCC 2 cut(s) 859, 894
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 3 cut(s) 443, 844, 914
BcuI ACTAGT 1 cut(s) 1042
BfaI CTAG 2 cut(s) 426, 1043
BfuI GTATCC 2 cut(s) 859, 894
BisI GCNGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 562
Bme1390I CCNGG 3 cut(s) 333, 454, 1181
Bme18I GGWCC 1 cut(s) 1025
BmeRI GACNNNNNGTC 1 cut(s) 1023
BmgT120I GGNCC 1 cut(s) 1025
BmiI GGNNCC 1 cut(s) 1185
BmrFI CCNGG 3 cut(s) 333, 454, 1181
BmrI ACTGGG 1 cut(s) 929
BmsI GCATC 7 cut(s) 169, 429, 451, 499, 646, 696, 942
BmuI ACTGGG 1 cut(s) 929
BpiI GAAGAC 4 cut(s) 201, 228, 489, 1149
Bpu10I CCTNAGC 1 cut(s) 660
BpuEI CTTGAG 1 cut(s) 1184
BpuMI CCSGG 1 cut(s) 333
Bsa29I ATCGAT 1 cut(s) 307
BsaBI GATNNNNATC 1 cut(s) 303
BsaHI GRCGYC 4 cut(s) 84, 120, 198, 267
BsaI GGTCTC 2 cut(s) 443, 844
BsaWI WCCGGW 1 cut(s) 835
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 996
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 2 cut(s) 935, 1082
Bse21I CCTNAGG 1 cut(s) 678
Bse3DI GCAATG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 303
BseBI CCWGG 2 cut(s) 454, 1181
BseCI ATCGAT 1 cut(s) 307
BseGI GGATG 6 cut(s) 184, 442, 661, 711, 1093, 1174
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 2 cut(s) 262, 996
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 5 cut(s) 651, 692, 915, 993, 1026
BseNI ACTGG 2 cut(s) 935, 1082
BseRI GAGGAG 6 cut(s) 386, 389, 392, 395, 398, 843
BseX3I CGGCCG 1 cut(s) 318
BseXI GCAGC 1 cut(s) 547
BseYI CCCAGC 1 cut(s) 513
Bsh1236I CGCG 3 cut(s) 208, 253, 437
Bsh1285I CGRYCG 2 cut(s) 307, 321
BshFI GGCC 4 cut(s) 60, 320, 419, 1081
BshNI GGYRCC 1 cut(s) 1183
BshVI ATCGAT 1 cut(s) 307
BsiEI CGRYCG 2 cut(s) 307, 321
BsiSI CCGG 3 cut(s) 317, 333, 836
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 262, 996
BsmAI GTCTC 3 cut(s) 443, 844, 914
BsmFI GGGAC 1 cut(s) 107
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 4 cut(s) 60, 320, 419, 1081
Bso31I GGTCTC 2 cut(s) 443, 844
Bsp143I GATC 5 cut(s) 64, 304, 308, 322, 1159
Bsp68I TCGCGA 1 cut(s) 437
BspACI CCGC 5 cut(s) 97, 125, 132, 293, 1188
BspANI GGCC 4 cut(s) 60, 320, 419, 1081
BspCNI CTCAG 5 cut(s) 652, 691, 916, 994, 1027
BspDI ATCGAT 1 cut(s) 307
BspFNI CGCG 3 cut(s) 208, 253, 437
BspLI GGNNCC 1 cut(s) 1185
BspPI GGATC 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 1183
BspTNI GGTCTC 2 cut(s) 443, 844
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 316
BsrI ACTGG 2 cut(s) 935, 1082
BssAI RCCGGY 1 cut(s) 316
BssMI GATC 5 cut(s) 64, 304, 308, 322, 1159
BssNI GRCGYC 4 cut(s) 84, 120, 198, 267
Bst2UI CCWGG 2 cut(s) 454, 1181
Bst4CI ACNGT 2 cut(s) 797, 1021
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 4 cut(s) 84, 120, 198, 267
BstC8I GCNNGC 3 cut(s) 12, 318, 525
BstDEI CTNAG 5 cut(s) 660, 678, 924, 1002, 1035
BstENI CCTNNNNNAGG 1 cut(s) 994
BstF5I GGATG 6 cut(s) 184, 442, 661, 711, 1093, 1174
BstFNI CGCG 3 cut(s) 208, 253, 437
BstHHI GCGC 4 cut(s) 255, 286, 392, 550
BstKTI GATC 5 cut(s) 67, 307, 311, 325, 1162
BstMAI GTCTC 3 cut(s) 443, 844, 914
BstMBI GATC 5 cut(s) 64, 304, 308, 322, 1159
BstMCI CGRYCG 2 cut(s) 307, 321
BstMWI GCNNNNNNNGC 4 cut(s) 292, 594, 640, 649
BstNI CCWGG 2 cut(s) 454, 1181
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 331, 452, 1179
BstUI CGCG 3 cut(s) 208, 253, 437
BstV1I GCAGC 1 cut(s) 547
BstV2I GAAGAC 4 cut(s) 201, 228, 489, 1149
BstXI CCANNNNNNTGG 2 cut(s) 209, 453
BstZI CGGCCG 1 cut(s) 318
Bsu15I ATCGAT 1 cut(s) 307
Bsu36I CCTNAGG 1 cut(s) 678
BsuI GTATCC 2 cut(s) 859, 894
BsuRI GGCC 4 cut(s) 60, 320, 419, 1081
BsuTUI ATCGAT 1 cut(s) 307
BtgZI GCGATG 2 cut(s) 418, 452
BtsCI GGATG 6 cut(s) 184, 442, 661, 711, 1093, 1174
BtsIMutI CAGTG 2 cut(s) 402, 1162
BtuMI TCGCGA 1 cut(s) 437
Cac8I GCNNGC 3 cut(s) 12, 318, 525
CfoI GCGC 4 cut(s) 255, 286, 392, 550
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 1025
ClaI ATCGAT 1 cut(s) 307
CseI GACGC 2 cut(s) 206, 256
CsiI ACCWGGT 1 cut(s) 452
Csp6I GTAC 1 cut(s) 89
CviAII CATG 5 cut(s) 6, 524, 758, 812, 901
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 5 cut(s) 660, 678, 924, 1002, 1035
DpnI GATC 5 cut(s) 66, 306, 310, 324, 1161
DpnII GATC 5 cut(s) 64, 304, 308, 322, 1159
DraI TTTAAA 1 cut(s) 789
DrdI GACNNNNNNGTC 1 cut(s) 83
DriI GACNNNNNGTC 1 cut(s) 1023
DseDI GACNNNNNNGTC 1 cut(s) 83
EaeI YGGCCR 2 cut(s) 318, 1079
EagI CGGCCG 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 363
Eam1105I GACNNNNNGTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 363
EclXI CGGCCG 1 cut(s) 318
Eco31I GGTCTC 2 cut(s) 443, 844
Eco47I GGWCC 1 cut(s) 1025
Eco52I CGGCCG 1 cut(s) 318
Eco81I CCTNAGG 1 cut(s) 678
EcoNI CCTNNNNNAGG 1 cut(s) 994
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 452, 1179
EcoT22I ATGCAT 1 cut(s) 444
FaeI CATG 5 cut(s) 9, 527, 761, 815, 904
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FaqI GGGAC 1 cut(s) 107
FatI CATG 5 cut(s) 5, 523, 757, 811, 900
FauI CCCGC 2 cut(s) 132, 1195
FauNDI CATATG 1 cut(s) 591
Fnu4HI GCNGC 1 cut(s) 561
FokI GGATG 6 cut(s) 191, 429, 668, 718, 1100, 1181
Fsp4HI GCNGC 1 cut(s) 561
FspBI CTAG 2 cut(s) 426, 1043
GlaI GCGC 4 cut(s) 254, 285, 391, 549
GluI GCNGC 1 cut(s) 561
GsaI CCCAGC 1 cut(s) 517
HaeIII GGCC 4 cut(s) 60, 320, 419, 1081
HapII CCGG 3 cut(s) 317, 333, 836
HgaI GACGC 2 cut(s) 206, 256
HhaI GCGC 4 cut(s) 255, 286, 392, 550
Hin1I GRCGYC 4 cut(s) 84, 120, 198, 267
Hin1II CATG 5 cut(s) 9, 527, 761, 815, 904
Hin6I GCGC 4 cut(s) 253, 284, 390, 548
HinP1I GCGC 4 cut(s) 253, 284, 390, 548
HinfI GANTC 3 cut(s) 570, 727, 893
HpaII CCGG 3 cut(s) 317, 333, 836
HphI GGTGA 3 cut(s) 317, 374, 1106
Hpy166II GTNNAC 1 cut(s) 672
Hpy188I TCNGA 7 cut(s) 178, 232, 313, 508, 726, 754, 1036
Hpy188III TCNNGA 6 cut(s) 68, 436, 446, 470, 610, 1163
Hpy8I GTNNAC 1 cut(s) 672
HpyAV CCTTC 5 cut(s) 50, 353, 430, 915, 1000
HpyCH4III ACNGT 2 cut(s) 797, 1021
HpyCH4IV ACGT 4 cut(s) 84, 120, 784, 881
HpyCH4V TGCA 5 cut(s) 182, 442, 634, 709, 806
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 594, 640, 649
HpyF3I CTNAG 5 cut(s) 660, 678, 924, 1002, 1035
HpySE526I ACGT 4 cut(s) 84, 120, 784, 881
Hsp92I GRCGYC 4 cut(s) 84, 120, 198, 267
Hsp92II CATG 5 cut(s) 9, 527, 761, 815, 904
HspAI GCGC 4 cut(s) 253, 284, 390, 548
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Kzo9I GATC 5 cut(s) 64, 304, 308, 322, 1159
Lsp1109I GCAGC 1 cut(s) 547
LweI GCATC 7 cut(s) 169, 429, 451, 499, 646, 696, 942
MabI ACCWGGT 1 cut(s) 452
MaeI CTAG 2 cut(s) 426, 1043
MaeII ACGT 4 cut(s) 84, 120, 784, 881
MaeIII GTNAC 3 cut(s) 400, 602, 1015
MalI GATC 5 cut(s) 66, 306, 310, 324, 1161
MboI GATC 5 cut(s) 64, 304, 308, 322, 1159
MlsI TGGCCA 1 cut(s) 1081
MluCI AATT 5 cut(s) 233, 257, 738, 1097, 1193
MluI ACGCGT 1 cut(s) 206
MluNI TGGCCA 1 cut(s) 1081
MlyI GAGTC 1 cut(s) 721
MmeI TCCRAC 2 cut(s) 438, 755
Mox20I TGGCCA 1 cut(s) 1081
Mph1103I ATGCAT 1 cut(s) 444
MroNI GCCGGC 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 778
MscI TGGCCA 1 cut(s) 1081
MseI TTAA 3 cut(s) 788, 1047, 1196
MslI CAYNNNNRTG 1 cut(s) 437
Msp20I TGGCCA 1 cut(s) 1081
MspA1I CMGCKG 1 cut(s) 1121
MspI CCGG 3 cut(s) 317, 333, 836
MspR9I CCNGG 3 cut(s) 333, 454, 1181
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 2 cut(s) 454, 1181
MvnI CGCG 3 cut(s) 208, 253, 437
MwoI GCNNNNNNNGC 4 cut(s) 292, 594, 640, 649
NaeI GCCGGC 1 cut(s) 318
NciI CCSGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 591
NdeII GATC 5 cut(s) 64, 304, 308, 322, 1159
NgoMIV GCCGGC 1 cut(s) 316
NlaIII CATG 5 cut(s) 9, 527, 761, 815, 904
NlaIV GGNNCC 1 cut(s) 1185
NmeAIII GCCGAG 1 cut(s) 576
NmuCI GTSAC 2 cut(s) 400, 1015
NruI TCGCGA 1 cut(s) 437
NsiI ATGCAT 1 cut(s) 444
NspI RCATGY 1 cut(s) 527
PaeI GCATGC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 6 cut(s) 51, 75, 78, 81, 84, 347
PctI GAATGC 1 cut(s) 634
PdiI GCCGGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 778
PfeI GAWTC 2 cut(s) 570, 893
PflFI GACNNNGTC 2 cut(s) 83, 410
PkrI GCNGC 1 cut(s) 562
Ple19I CGATCG 1 cut(s) 307
PleI GAGTC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 721
Psp6I CCWGG 2 cut(s) 452, 1179
PspFI CCCAGC 1 cut(s) 513
PspGI CCWGG 2 cut(s) 452, 1179
PspN4I GGNNCC 1 cut(s) 1185
PspPI GGNCC 1 cut(s) 1025
PsyI GACNNNGTC 2 cut(s) 83, 410
PvuI CGATCG 1 cut(s) 307
PvuII CAGCTG 1 cut(s) 1121
RruI TCGCGA 1 cut(s) 437
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 437
SaqAI TTAA 3 cut(s) 788, 1047, 1196
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 5 cut(s) 64, 304, 308, 322, 1159
Sau96I GGNCC 1 cut(s) 1025
SchI GAGTC 1 cut(s) 721
ScrFI CCNGG 3 cut(s) 333, 454, 1181
SexAI ACCWGGT 1 cut(s) 452
SfaNI GCATC 7 cut(s) 169, 429, 451, 499, 646, 696, 942
SinI GGWCC 1 cut(s) 1025
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
SpeI ACTAGT 1 cut(s) 1042
SphI GCATGC 1 cut(s) 527
Sse9I AATT 5 cut(s) 233, 257, 738, 1097, 1193
SsiI CCGC 5 cut(s) 97, 125, 132, 293, 1188
SspMI CTAG 2 cut(s) 426, 1043
StyD4I CCNGG 3 cut(s) 331, 452, 1179
TaaI ACNGT 2 cut(s) 797, 1021
TaiI ACGT 4 cut(s) 87, 123, 787, 884
TaqI TCGA 5 cut(s) 42, 307, 573, 638, 978
TasI AATT 5 cut(s) 233, 257, 738, 1097, 1193
TfiI GAWTC 2 cut(s) 570, 893
Tru1I TTAA 3 cut(s) 788, 1047, 1196
Tru9I TTAA 3 cut(s) 788, 1047, 1196
TscAI CASTG 2 cut(s) 409, 1162
TseFI GTSAC 2 cut(s) 400, 1015
TseI GCWGC 1 cut(s) 560
Tsp45I GTSAC 2 cut(s) 400, 1015
TspGWI ACGGA 4 cut(s) 26, 573, 961, 1017
TspRI CASTG 2 cut(s) 409, 1162
Tth111I GACNNNGTC 2 cut(s) 83, 410
VpaK11BI GGWCC 1 cut(s) 1025
XagI CCTNNNNNAGG 1 cut(s) 994
XapI RAATTY 1 cut(s) 233
XceI RCATGY 1 cut(s) 527
XmnI GAANNNNTTC 1 cut(s) 778
XspI CTAG 2 cut(s) 426, 1043
ZraI GACGTC 2 cut(s) 85, 121
Zsp2I ATGCAT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.