RLG00000010525

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010000
Physical Location & Seq
Reverse (-)
2377261 .. 2380107
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010525

Sequence Viewer

Length: 1236 bp
ATGTCATGGCTTGCCCACTCGCTCTCTAAATCCCTCCGTATCGACGGCGACGACGGTGACGGCGAAGGCCAAGATCACGACGACGACGTCGTACCCCACCACCACACCAACAATGAAGCTGACGTCCCGCCCACCGCCTCCACCAAACCCCGAGACAACGAAATCCAATCGGATGCAGAAGCAGTTGAAGACGCCCAAACGCGTGGCGTGAAAGAAGACCTATCGGAATTCCAGAAAACCCTAACGCGCCAATTCTGGGGCGTCGCCTCGTTCCTTGCGCCACCACCGCCCTCTGATCGATTTGCTTCTCCGCCCGATTGCGATCGATCCGAGCCGGCCGATCACCCACCCGGTCAATCGCCGATGGCGTTCAATGAAGACGAAGAAGAAGAGGAGGAGGAGGAGTGCGCCGTCGGTGTCACTGACGAGGTCTTGGCCTTCGCTAGAAACATCGCGATGCATCCAGAGACCTGGTTGGACTTTCCTCTTGACGAAGAAGAAGACTTGGGAGATTTTGAAATGTCTGATGCCCAGCAAGAGCATGCTATGATGATTGAACATCTTGCGCCGAGATTAGCTGCTTTGAGAATCGAGCTTTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTGTATTTTGTTCTTCTGCATTCGAGGCTCAACAAGCCGGATGCTGAGGTTTTGTCCACACCTCAGGTAATGGAAGCCAGAGCAATGTGGATGCAAGAGCTACAATGTCAGACTCCAACATCAATTTCTAAAATGTCAGAACATGAATGGTTTGGAAGAAGCACTTCACATTTAAAATACAGTTCAAATGCAATACATGAAGATTATGAAGATTACTCTCCGGTCTCATCACATACTCCTCATTATGAGGATACACTTCATAAGACGTATCCTTTTGAATCAACCATGCCTTCCACAGCCGATGAGACTGAGAAGCACCCAGTTGAGAGTAATGAGATGCCATTCATTGATAAGTCCGTTATCGAAGAAAAGCCCTTCATTAGGACTGAGGATAAGAATGTGACAGTTGGTCCATCCTCTGAGATACTAGTTAATAGCTATGACGATGACGACGATGATGACTGGCCAGAGGATGATGGTGAATTATATGGGTGTAGTGGAACAGCTGTTGCTTTGATAAATGAAGAAGACATATCTTTCAGTGATCTTGAGGATGATGATTTCCAGGCACCCTCTAAATTAAAGTTCATCTCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

412

Amino Acids

46.55

Weight (kDa)

4.26

Isoelectric Point (pI)

65.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 170 - 223 5.6e-13 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 2 cut(s) 90, 126
AccB1I GGYRCC 1 cut(s) 1201
AccII CGCG 3 cut(s) 202, 247, 455
AciI CCGC 4 cut(s) 128, 135, 287, 311
AclWI GGATC 1 cut(s) 321
AcoI YGGCCR 2 cut(s) 336, 1097
AcsI RAATTY 1 cut(s) 227
AcyI GRCGYC 4 cut(s) 87, 123, 192, 261
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 256, 1014
AflIII ACRYGT 1 cut(s) 200
AgsI TTSAA 6 cut(s) 188, 373, 518, 557, 819, 911
AhdI GACNNNNNGTC 1 cut(s) 1041
AhlI ACTAGT 1 cut(s) 1060
AjnI CCWGG 2 cut(s) 470, 1197
AjuI GAANNNNNNNTTGG 2 cut(s) 742, 774
AluBI AGCT 6 cut(s) 119, 578, 595, 733, 1071, 1139
AluI AGCT 6 cut(s) 119, 578, 595, 733, 1071, 1139
Alw26I GTCTC 4 cut(s) 147, 461, 862, 932
AlwI GGATC 1 cut(s) 321
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 4 cut(s) 67, 336, 435, 1097
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 796
AspLEI GCGC 4 cut(s) 249, 280, 410, 568
AspS9I GGNCC 1 cut(s) 1043
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 3 cut(s) 68, 335, 1124
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 1 cut(s) 1043
AxyI CCTNAGG 1 cut(s) 696
BalI TGGCCA 1 cut(s) 1099
BanI GGYRCC 1 cut(s) 1201
BbsI GAAGAC 5 cut(s) 195, 222, 384, 507, 1167
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 1 cut(s) 565
BccI CCATC 3 cut(s) 358, 1054, 1103
BceAI ACGGC 3 cut(s) 61, 76, 395
BciT130I CCWGG 2 cut(s) 472, 1199
BciVI GTATCC 2 cut(s) 877, 912
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 4 cut(s) 147, 461, 862, 932
BcuI ACTAGT 1 cut(s) 1060
BfaI CTAG 2 cut(s) 444, 1061
BfuI GTATCC 2 cut(s) 877, 912
BisI GCNGC 1 cut(s) 579
BlsI GCNGC 1 cut(s) 580
Bme1390I CCNGG 3 cut(s) 351, 472, 1199
Bme18I GGWCC 1 cut(s) 1043
BmeRI GACNNNNNGTC 1 cut(s) 1041
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 1043
BmiI GGNNCC 1 cut(s) 1203
BmrFI CCNGG 3 cut(s) 351, 472, 1199
BmrI ACTGGG 1 cut(s) 947
BmsI GCATC 7 cut(s) 163, 447, 469, 517, 664, 714, 960
BmuI ACTGGG 1 cut(s) 947
BpiI GAAGAC 5 cut(s) 195, 222, 384, 507, 1167
Bpu10I CCTNAGC 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 1202
BpuMI CCSGG 1 cut(s) 351
Bsa29I ATCGAT 2 cut(s) 298, 325
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 4 cut(s) 87, 123, 192, 261
BsaI GGTCTC 2 cut(s) 461, 862
BsaWI WCCGGW 1 cut(s) 853
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 1014
Bse118I RCCGGY 1 cut(s) 334
Bse1I ACTGG 2 cut(s) 953, 1100
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 723
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 2 cut(s) 472, 1199
BseCI ATCGAT 2 cut(s) 298, 325
BseGI GGATG 7 cut(s) 178, 460, 679, 729, 1046, 1111, 1192
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 256, 1014
BseMI GCAATG 1 cut(s) 723
BseMII CTCAG 5 cut(s) 669, 710, 933, 1011, 1044
BseNI ACTGG 2 cut(s) 953, 1100
BseRI GAGGAG 5 cut(s) 407, 410, 413, 416, 861
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 565
BseYI CCCAGC 1 cut(s) 531
Bsh1236I CGCG 3 cut(s) 202, 247, 455
Bsh1285I CGRYCG 2 cut(s) 325, 339
BshFI GGCC 4 cut(s) 69, 338, 437, 1099
BshNI GGYRCC 1 cut(s) 1201
BshVI ATCGAT 2 cut(s) 298, 325
BsiEI CGRYCG 2 cut(s) 325, 339
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 4 cut(s) 335, 351, 671, 854
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 256, 1014
BsmAI GTCTC 4 cut(s) 147, 461, 862, 932
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 4 cut(s) 69, 338, 437, 1099
Bso31I GGTCTC 2 cut(s) 461, 862
BsoBI CYCGRG 1 cut(s) 150
Bsp143I GATC 6 cut(s) 73, 295, 322, 326, 340, 1177
Bsp68I TCGCGA 1 cut(s) 455
BspACI CCGC 4 cut(s) 128, 135, 287, 311
BspANI GGCC 4 cut(s) 69, 338, 437, 1099
BspCNI CTCAG 5 cut(s) 670, 709, 934, 1012, 1045
BspDI ATCGAT 2 cut(s) 298, 325
BspFNI CGCG 3 cut(s) 202, 247, 455
BspLI GGNNCC 1 cut(s) 1203
BspPI GGATC 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 1201
BspTNI GGTCTC 2 cut(s) 461, 862
BsrDI GCAATG 1 cut(s) 723
BsrFI RCCGGY 1 cut(s) 334
BsrI ACTGG 2 cut(s) 953, 1100
BssAI RCCGGY 1 cut(s) 334
BssMI GATC 6 cut(s) 73, 295, 322, 326, 340, 1177
BssNI GRCGYC 4 cut(s) 87, 123, 192, 261
Bst2UI CCWGG 2 cut(s) 472, 1199
Bst4CI ACNGT 3 cut(s) 56, 815, 1039
Bst6I CTCTTC 1 cut(s) 384
BstACI GRCGYC 4 cut(s) 87, 123, 192, 261
BstC8I GCNNGC 3 cut(s) 12, 336, 543
BstDEI CTNAG 5 cut(s) 678, 696, 942, 1020, 1053
BstENI CCTNNNNNAGG 1 cut(s) 1012
BstF5I GGATG 7 cut(s) 178, 460, 679, 729, 1046, 1111, 1192
BstFNI CGCG 3 cut(s) 202, 247, 455
BstHHI GCGC 4 cut(s) 249, 280, 410, 568
BstKTI GATC 6 cut(s) 76, 298, 325, 329, 343, 1180
BstMAI GTCTC 4 cut(s) 147, 461, 862, 932
BstMBI GATC 6 cut(s) 73, 295, 322, 326, 340, 1177
BstMCI CGRYCG 2 cut(s) 325, 339
BstMWI GCNNNNNNNGC 4 cut(s) 286, 612, 658, 667
BstNI CCWGG 2 cut(s) 472, 1199
BstNSI RCATGY 1 cut(s) 545
BstSCI CCNGG 3 cut(s) 349, 470, 1197
BstUI CGCG 3 cut(s) 202, 247, 455
BstV1I GCAGC 1 cut(s) 565
BstV2I GAAGAC 5 cut(s) 195, 222, 384, 507, 1167
BstXI CCANNNNNNTGG 2 cut(s) 203, 471
BstZI CGGCCG 1 cut(s) 336
Bsu15I ATCGAT 2 cut(s) 298, 325
Bsu36I CCTNAGG 1 cut(s) 696
BsuI GTATCC 2 cut(s) 877, 912
BsuRI GGCC 4 cut(s) 69, 338, 437, 1099
BsuTUI ATCGAT 2 cut(s) 298, 325
BtgZI GCGATG 2 cut(s) 436, 470
BtsCI GGATG 7 cut(s) 178, 460, 679, 729, 1046, 1111, 1192
BtsIMutI CAGTG 2 cut(s) 420, 1180
BtuMI TCGCGA 1 cut(s) 455
Cac8I GCNNGC 3 cut(s) 12, 336, 543
CfoI GCGC 4 cut(s) 249, 280, 410, 568
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 1043
ClaI ATCGAT 2 cut(s) 298, 325
CseI GACGC 2 cut(s) 200, 250
CsiI ACCWGGT 1 cut(s) 470
Csp6I GTAC 1 cut(s) 92
CviAII CATG 5 cut(s) 6, 542, 776, 830, 919
CviQI GTAC 1 cut(s) 92
DdeI CTNAG 5 cut(s) 678, 696, 942, 1020, 1053
DpnI GATC 6 cut(s) 75, 297, 324, 328, 342, 1179
DpnII GATC 6 cut(s) 73, 295, 322, 326, 340, 1177
DraI TTTAAA 1 cut(s) 807
DrdI GACNNNNNNGTC 1 cut(s) 86
DriI GACNNNNNGTC 1 cut(s) 1041
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 2 cut(s) 336, 1097
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 384
Eam1105I GACNNNNNGTC 1 cut(s) 1041
EarI CTCTTC 1 cut(s) 384
EciI GGCGGA 1 cut(s) 300
EclXI CGGCCG 1 cut(s) 336
Eco31I GGTCTC 2 cut(s) 461, 862
Eco47I GGWCC 1 cut(s) 1043
Eco52I CGGCCG 1 cut(s) 336
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 150
EcoNI CCTNNNNNAGG 1 cut(s) 1012
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 470, 1197
EcoT22I ATGCAT 1 cut(s) 462
FaeI CATG 5 cut(s) 9, 545, 779, 833, 922
FalI AAGNNNNNCTT 2 cut(s) 781, 813
FaqI GGGAC 1 cut(s) 110
FatI CATG 5 cut(s) 5, 541, 775, 829, 918
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 1 cut(s) 579
FokI GGATG 7 cut(s) 185, 447, 686, 736, 1033, 1118, 1199
Fsp4HI GCNGC 1 cut(s) 579
FspBI CTAG 2 cut(s) 444, 1061
GlaI GCGC 4 cut(s) 248, 279, 409, 567
GluI GCNGC 1 cut(s) 579
GsaI CCCAGC 1 cut(s) 535
HaeIII GGCC 4 cut(s) 69, 338, 437, 1099
HapII CCGG 4 cut(s) 335, 351, 671, 854
HgaI GACGC 2 cut(s) 200, 250
HhaI GCGC 4 cut(s) 249, 280, 410, 568
Hin1I GRCGYC 4 cut(s) 87, 123, 192, 261
Hin1II CATG 5 cut(s) 9, 545, 779, 833, 922
Hin6I GCGC 4 cut(s) 247, 278, 408, 566
HinP1I GCGC 4 cut(s) 247, 278, 408, 566
HinfI GANTC 3 cut(s) 588, 745, 911
HpaII CCGG 4 cut(s) 335, 351, 671, 854
HphI GGTGA 3 cut(s) 68, 335, 1124
Hpy166II GTNNAC 1 cut(s) 690
Hpy188I TCNGA 8 cut(s) 172, 226, 295, 331, 526, 744, 772, 1054
Hpy188III TCNNGA 7 cut(s) 77, 232, 454, 464, 488, 628, 1181
Hpy8I GTNNAC 1 cut(s) 690
HpyAV CCTTC 4 cut(s) 59, 448, 933, 1018
HpyCH4III ACNGT 3 cut(s) 56, 815, 1039
HpyCH4IV ACGT 3 cut(s) 87, 123, 899
HpyCH4V TGCA 5 cut(s) 176, 460, 652, 727, 824
HpyF10VI GCNNNNNNNGC 4 cut(s) 286, 612, 658, 667
HpyF3I CTNAG 5 cut(s) 678, 696, 942, 1020, 1053
HpySE526I ACGT 3 cut(s) 87, 123, 899
Hsp92I GRCGYC 4 cut(s) 87, 123, 192, 261
Hsp92II CATG 5 cut(s) 9, 545, 779, 833, 922
HspAI GCGC 4 cut(s) 247, 278, 408, 566
KroI GCCGGC 1 cut(s) 334
KroNI GCCGGC 1 cut(s) 336
Kzo9I GATC 6 cut(s) 73, 295, 322, 326, 340, 1177
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 7 cut(s) 163, 447, 469, 517, 664, 714, 960
MabI ACCWGGT 1 cut(s) 470
MaeI CTAG 2 cut(s) 444, 1061
MaeII ACGT 3 cut(s) 87, 123, 899
MaeIII GTNAC 4 cut(s) 56, 418, 620, 1033
MalI GATC 6 cut(s) 75, 297, 324, 328, 342, 1179
MboI GATC 6 cut(s) 73, 295, 322, 326, 340, 1177
MlsI TGGCCA 1 cut(s) 1099
MluCI AATT 5 cut(s) 227, 251, 756, 1115, 1211
MluI ACGCGT 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 1099
MlyI GAGTC 1 cut(s) 739
MmeI TCCRAC 2 cut(s) 456, 773
Mox20I TGGCCA 1 cut(s) 1099
Mph1103I ATGCAT 1 cut(s) 462
MroNI GCCGGC 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 796
MscI TGGCCA 1 cut(s) 1099
MseI TTAA 3 cut(s) 806, 1065, 1214
MslI CAYNNNNRTG 1 cut(s) 455
Msp20I TGGCCA 1 cut(s) 1099
MspA1I CMGCKG 1 cut(s) 1139
MspI CCGG 4 cut(s) 335, 351, 671, 854
MspR9I CCNGG 3 cut(s) 351, 472, 1199
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 2 cut(s) 472, 1199
MvnI CGCG 3 cut(s) 202, 247, 455
MwoI GCNNNNNNNGC 4 cut(s) 286, 612, 658, 667
NaeI GCCGGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 351
NdeI CATATG 1 cut(s) 609
NdeII GATC 6 cut(s) 73, 295, 322, 326, 340, 1177
NgoMIV GCCGGC 1 cut(s) 334
NlaIII CATG 5 cut(s) 9, 545, 779, 833, 922
NlaIV GGNNCC 1 cut(s) 1203
NmeAIII GCCGAG 1 cut(s) 594
NmuCI GTSAC 3 cut(s) 56, 418, 1033
NruI TCGCGA 1 cut(s) 455
NsiI ATGCAT 1 cut(s) 462
NspI RCATGY 1 cut(s) 545
PaeI GCATGC 1 cut(s) 545
PcsI WCGNNNNNNNCGW 6 cut(s) 48, 60, 84, 87, 365, 1083
PctI GAATGC 1 cut(s) 652
PdiI GCCGGC 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 796
PfeI GAWTC 2 cut(s) 588, 911
PflFI GACNNNGTC 2 cut(s) 86, 428
PkrI GCNGC 1 cut(s) 580
Ple19I CGATCG 1 cut(s) 325
PleI GAGTC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 739
Psp6I CCWGG 2 cut(s) 470, 1197
PspFI CCCAGC 1 cut(s) 531
PspGI CCWGG 2 cut(s) 470, 1197
PspN4I GGNNCC 1 cut(s) 1203
PspPI GGNCC 1 cut(s) 1043
PsyI GACNNNGTC 2 cut(s) 86, 428
PvuI CGATCG 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 1139
RruI TCGCGA 1 cut(s) 455
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 3 cut(s) 806, 1065, 1214
SatI GCNGC 1 cut(s) 579
Sau3AI GATC 6 cut(s) 73, 295, 322, 326, 340, 1177
Sau96I GGNCC 1 cut(s) 1043
SchI GAGTC 1 cut(s) 739
ScrFI CCNGG 3 cut(s) 351, 472, 1199
SexAI ACCWGGT 1 cut(s) 470
SfaNI GCATC 7 cut(s) 163, 447, 469, 517, 664, 714, 960
SinI GGWCC 1 cut(s) 1043
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 1181
SmoI CTYRAG 1 cut(s) 1181
SpeI ACTAGT 1 cut(s) 1060
SphI GCATGC 1 cut(s) 545
Sse9I AATT 5 cut(s) 227, 251, 756, 1115, 1211
SsiI CCGC 4 cut(s) 128, 135, 287, 311
SspMI CTAG 2 cut(s) 444, 1061
StyD4I CCNGG 3 cut(s) 349, 470, 1197
TaaI ACNGT 3 cut(s) 56, 815, 1039
TaiI ACGT 3 cut(s) 90, 126, 902
TaqI TCGA 6 cut(s) 42, 298, 325, 591, 656, 996
TasI AATT 5 cut(s) 227, 251, 756, 1115, 1211
TfiI GAWTC 2 cut(s) 588, 911
Tru1I TTAA 3 cut(s) 806, 1065, 1214
Tru9I TTAA 3 cut(s) 806, 1065, 1214
TscAI CASTG 2 cut(s) 427, 1180
TseFI GTSAC 3 cut(s) 56, 418, 1033
TseI GCWGC 1 cut(s) 578
Tsp45I GTSAC 3 cut(s) 56, 418, 1033
TspGWI ACGGA 3 cut(s) 26, 591, 979
TspRI CASTG 2 cut(s) 427, 1180
Tth111I GACNNNGTC 2 cut(s) 86, 428
VpaK11BI GGWCC 1 cut(s) 1043
XagI CCTNNNNNAGG 1 cut(s) 1012
XapI RAATTY 1 cut(s) 227
XceI RCATGY 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 796
XspI CTAG 2 cut(s) 444, 1061
ZraI GACGTC 2 cut(s) 88, 124
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.