MD08G1079700.v1.1

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010000
Physical Location & Seq
Reverse (-)
6634599 .. 6637188
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1079700.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGTCATGGCTCGCTCGCTCCCTCGGTGACTCTCTACGTCTCAACGACGACGGCGACGCCGAGAACGACGCCTTACCCCACGACAACGACAATGGATCACCAACCCCACCCAGCGCTGACAACGACGTCGTTCGAAGAAGAAGCGAAATTGAATCTTCAGATGAGGAAAACGAAGACGAGGACGACCAAACCCGAGGCGTGAGGGAAGATTTGGACGAATTCAAGCAGACGCTGACCCGCCAATTCTGGGGCGTCGCGTCGTTCCTAGCACCGCCGCCTCCCCCTCCGCCACCTCCGTACCCTTCATTGCCGTCCAGTAATCGATCGAATCCTCCTGACATGGATCGATCCGAGCCGCCCGATCAATCGGCGATGGATTTTGATGAAGGTGAAGAGAAGGAGGAGGAATTGGAGGGGGATTGCGCCGTTGGGGTCACGGAGGAGGTATTGGCGTTCGCCAGGAACATCGCGATGCATCCGGAGACGTGGCTGGACTTTCCTCTCGACGAGGAGGAGGATTTGGGCGATTTTGACATGTCTGATGCCCAGCAAGAGCATGCTATGGTGATTGAACATCTTGCGCCAAGATTTGCTGCTTTGAGATTTGAACTGTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCAAGGCTCAATAAGCAGGATGCCGAGGTCTTATCCACACCCCAAGTAATGGAAGCCAGAGCAAAGTGGATGCAGGAGCTACATTGTCAGACTCCATTAGAACATGATCAATTTGGAAGAAACACTTCTTTTTTAAAAGACAGTTCCAATATAGTAGATGAAGATTGTGCTCCGGTCTCATCATGTAGTGTTTATTACGAGGACAAGATGCTAAGGACGTATCCTTATGAATCAACGATGTCCCCCACGGCTTATGAGACTGAGAAGCACCCAGTTGAGAGTGATGAAGTCCCATATATTGACAAGTCTGTTATTGAAGAAAAGCCCATCATTAAGAGCGAGGACAAGAATCTAACAGTTGGTCCATCCTCGAAGATGCTAGATAATATCTACGAAGATGATGATGATGATGACTGGCCAGAGGAAGATTCTGAATTAGATGGGTATAGTGGAACGGCTATTCACGCAATCAATGAAGAAGACATATCTTTCAGTGACCTAGAGGACGATGATTTCCTTGCTCCCACAAAACTGAAGACTGTCAAGAAACCTCAAGGAAAGAGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

413

Amino Acids

46.77

Weight (kDa)

4.19

Isoelectric Point (pI)

71.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 175 - 230 2.3e-12 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AatII GACGTC 1 cut(s) 129
AccB7I CCANNNNNTGG 1 cut(s) 718
AccII CGCG 2 cut(s) 257, 470
AccIII TCCGGA 1 cut(s) 478
AciI CCGC 5 cut(s) 238, 272, 275, 287, 356
AclWI GGATC 3 cut(s) 103, 342, 351
AcoI YGGCCR 1 cut(s) 1085
AcsI RAATTY 1 cut(s) 218
AcuI CTGAAG 2 cut(s) 141, 1223
AcyI GRCGYC 4 cut(s) 57, 69, 126, 252
AfaI GTAC 1 cut(s) 299
AfeI AGCGCT 1 cut(s) 115
AfiI CCNNNNNNNGG 2 cut(s) 247, 718
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 6 cut(s) 152, 223, 572, 608, 672, 986
AjiI CACGTC 1 cut(s) 486
AjnI CCWGG 1 cut(s) 458
AluBI AGCT 1 cut(s) 748
AluI AGCT 1 cut(s) 748
Alw21I GWGCWC 1 cut(s) 841
Alw26I GTCTC 4 cut(s) 44, 476, 850, 920
AlwI GGATC 3 cut(s) 103, 342, 351
AlwNI CAGNNNCTG 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 192
Aor13HI TCCGGA 1 cut(s) 478
Aor51HI AGCGCT 1 cut(s) 115
AoxI GGCC 1 cut(s) 1085
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 793
AspLEI GCGC 3 cut(s) 116, 425, 583
AspS9I GGNCC 1 cut(s) 1031
AsuHPI GGTGA 4 cut(s) 38, 90, 401, 577
AsuII TTCGAA 1 cut(s) 133
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 1 cut(s) 1031
BaeI ACNNNNGTAYC 2 cut(s) 281, 314
BalI TGGCCA 1 cut(s) 1087
BbsI GAAGAC 3 cut(s) 180, 1155, 1211
Bbv12I GWGCWC 1 cut(s) 841
BbvI GCAGC 1 cut(s) 580
BccI CCATC 4 cut(s) 367, 1004, 1042, 1103
BceAI ACGGC 5 cut(s) 67, 295, 410, 933, 1140
BciT130I CCWGG 1 cut(s) 460
BciVI GTATCC 1 cut(s) 900
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 4 cut(s) 44, 476, 850, 920
BfaI CTAG 3 cut(s) 266, 1049, 1169
BfoI RGCGCY 1 cut(s) 117
BfuI GTATCC 1 cut(s) 900
BisI GCNGC 3 cut(s) 275, 356, 594
BlsI GCNGC 3 cut(s) 276, 357, 595
Bme1390I CCNGG 1 cut(s) 460
Bme18I GGWCC 1 cut(s) 1031
BmeT110I CYCGRG 1 cut(s) 192
BmgBI CACGTC 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 1031
BmrFI CCNGG 1 cut(s) 460
BmrI ACTGGG 1 cut(s) 935
BmsI GCATC 7 cut(s) 462, 484, 532, 679, 729, 867, 1035
BmuI ACTGGG 1 cut(s) 935
BpiI GAAGAC 3 cut(s) 180, 1155, 1211
Bpu10I CCTNAGC 1 cut(s) 881
Bpu14I TTCGAA 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 1206
Bsa29I ATCGAT 2 cut(s) 322, 346
BsaHI GRCGYC 4 cut(s) 57, 69, 126, 252
BsaI GGTCTC 1 cut(s) 850
BsaJI CCNNGG 4 cut(s) 22, 193, 693, 915
BsaWI WCCGGW 2 cut(s) 478, 841
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 718
Bse1I ACTGG 3 cut(s) 315, 941, 1088
Bse3DI GCAATG 1 cut(s) 305
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 1 cut(s) 460
BseCI ATCGAT 2 cut(s) 322, 346
BseDI CCNNGG 4 cut(s) 22, 193, 693, 915
BseGI GGATG 4 cut(s) 475, 694, 744, 1034
BseLI CCNNNNNNNGG 2 cut(s) 247, 718
BseMI GCAATG 1 cut(s) 305
BseMII CTCAG 1 cut(s) 921
BseNI ACTGG 3 cut(s) 315, 941, 1088
BseRI GAGGAG 4 cut(s) 416, 455, 524, 527
BseXI GCAGC 1 cut(s) 580
BseYI CCCAGC 2 cut(s) 110, 546
Bsh1236I CGCG 2 cut(s) 257, 470
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 1 cut(s) 1087
BshVI ATCGAT 2 cut(s) 322, 346
BsiEI CGRYCG 1 cut(s) 326
BsiHKAI GWGCWC 1 cut(s) 841
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 2 cut(s) 479, 842
BslFI GGGAC 2 cut(s) 895, 944
BslI CCNNNNNNNGG 2 cut(s) 247, 718
BsmAI GTCTC 4 cut(s) 44, 476, 850, 920
BsmBI CGTCTC 2 cut(s) 44, 476
BsmFI GGGAC 2 cut(s) 895, 944
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 1 cut(s) 1087
Bso31I GGTCTC 1 cut(s) 850
BsoBI CYCGRG 1 cut(s) 192
Bsp119I TTCGAA 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 841
Bsp13I TCCGGA 1 cut(s) 478
Bsp143I GATC 6 cut(s) 95, 323, 343, 347, 361, 775
Bsp68I TCGCGA 1 cut(s) 470
BspACI CCGC 5 cut(s) 238, 272, 275, 287, 356
BspANI GGCC 1 cut(s) 1087
BspCNI CTCAG 1 cut(s) 922
BspDI ATCGAT 2 cut(s) 322, 346
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 2 cut(s) 257, 470
BspPI GGATC 3 cut(s) 103, 342, 351
BspT104I TTCGAA 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 850
BsrDI GCAATG 1 cut(s) 305
BsrI ACTGG 3 cut(s) 315, 941, 1088
BssECI CCNNGG 4 cut(s) 22, 193, 693, 915
BssMI GATC 6 cut(s) 95, 323, 343, 347, 361, 775
BssNI GRCGYC 4 cut(s) 57, 69, 126, 252
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 4 cut(s) 612, 812, 1027, 1210
Bst6I CTCTTC 1 cut(s) 387
BstACI GRCGYC 4 cut(s) 57, 69, 126, 252
BstBI TTCGAA 1 cut(s) 133
BstC8I GCNNGC 3 cut(s) 12, 16, 558
BstDEI CTNAG 2 cut(s) 881, 930
BstDSI CCRYGG 1 cut(s) 915
BstF5I GGATG 4 cut(s) 475, 694, 744, 1034
BstFNI CGCG 2 cut(s) 257, 470
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 3 cut(s) 116, 425, 583
BstKTI GATC 6 cut(s) 98, 326, 346, 350, 364, 778
BstMAI GTCTC 4 cut(s) 44, 476, 850, 920
BstMBI GATC 6 cut(s) 95, 323, 343, 347, 361, 775
BstMCI CGRYCG 1 cut(s) 326
BstMWI GCNNNNNNNGC 4 cut(s) 627, 673, 682, 1133
BstNI CCWGG 1 cut(s) 460
BstNSI RCATGY 2 cut(s) 538, 560
BstSCI CCNGG 1 cut(s) 458
BstUI CGCG 2 cut(s) 257, 470
BstV1I GCAGC 1 cut(s) 580
BstV2I GAAGAC 3 cut(s) 180, 1155, 1211
Bsu15I ATCGAT 2 cut(s) 322, 346
BsuI GTATCC 1 cut(s) 900
BsuRI GGCC 1 cut(s) 1087
BsuTUI ATCGAT 2 cut(s) 322, 346
BtgI CCRYGG 1 cut(s) 915
BtgZI GCGATG 3 cut(s) 386, 451, 485
BtrI CACGTC 1 cut(s) 486
BtsCI GGATG 4 cut(s) 475, 694, 744, 1034
BtsIMutI CAGTG 1 cut(s) 1168
BtuMI TCGCGA 1 cut(s) 470
Cac8I GCNNGC 3 cut(s) 12, 16, 558
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 3 cut(s) 116, 425, 583
Cfr13I GGNCC 1 cut(s) 1031
ClaI ATCGAT 2 cut(s) 322, 346
CseI GACGC 5 cut(s) 65, 77, 238, 241, 246
Csp6I GTAC 1 cut(s) 298
CviAII CATG 6 cut(s) 6, 340, 535, 557, 773, 852
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 2 cut(s) 881, 930
DpnI GATC 6 cut(s) 97, 325, 345, 349, 363, 777
DpnII GATC 6 cut(s) 95, 323, 343, 347, 361, 775
DraI TTTAAA 1 cut(s) 804
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 1085
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
EciI GGCGGA 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 850
Eco47I GGWCC 1 cut(s) 1031
Eco47III AGCGCT 1 cut(s) 115
Eco57I CTGAAG 2 cut(s) 141, 1223
Eco88I CYCGRG 1 cut(s) 192
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 458
EcoT22I ATGCAT 1 cut(s) 477
Esp3I CGTCTC 2 cut(s) 44, 476
FaeI CATG 6 cut(s) 9, 343, 538, 560, 776, 855
FalI AAGNNNNNCTT 2 cut(s) 778, 810
FaqI GGGAC 2 cut(s) 895, 944
FatI CATG 6 cut(s) 5, 339, 534, 556, 772, 851
FauI CCCGC 1 cut(s) 245
FauNDI CATATG 1 cut(s) 624
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 3 cut(s) 275, 356, 594
FokI GGATG 4 cut(s) 462, 701, 751, 1021
Fsp4HI GCNGC 3 cut(s) 275, 356, 594
FspBI CTAG 3 cut(s) 266, 1049, 1169
GlaI GCGC 3 cut(s) 115, 424, 582
GluI GCNGC 3 cut(s) 275, 356, 594
GsaI CCCAGC 2 cut(s) 114, 550
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 1 cut(s) 1087
HapII CCGG 2 cut(s) 479, 842
HgaI GACGC 5 cut(s) 65, 77, 238, 241, 246
HhaI GCGC 3 cut(s) 116, 425, 583
Hin1I GRCGYC 4 cut(s) 57, 69, 126, 252
Hin1II CATG 6 cut(s) 9, 343, 538, 560, 776, 855
Hin6I GCGC 3 cut(s) 114, 423, 581
HinP1I GCGC 3 cut(s) 114, 423, 581
HinfI GANTC 7 cut(s) 29, 152, 328, 760, 899, 1018, 1097
HpaII CCGG 2 cut(s) 479, 842
HphI GGTGA 4 cut(s) 38, 90, 401, 577
Hpy188I TCNGA 5 cut(s) 160, 352, 541, 759, 1102
Hpy188III TCNNGA 6 cut(s) 335, 469, 479, 503, 643, 1213
Hpy99I CGWCG 9 cut(s) 50, 53, 59, 71, 128, 131, 257, 262, 509
HpyAV CCTTC 3 cut(s) 312, 380, 391
HpyCH4III ACNGT 4 cut(s) 612, 812, 1027, 1210
HpyCH4IV ACGT 4 cut(s) 37, 126, 485, 887
HpyCH4V TGCA 3 cut(s) 475, 667, 742
HpyF10VI GCNNNNNNNGC 4 cut(s) 627, 673, 682, 1133
HpyF3I CTNAG 2 cut(s) 881, 930
HpySE526I ACGT 4 cut(s) 37, 126, 485, 887
Hsp92I GRCGYC 4 cut(s) 57, 69, 126, 252
Hsp92II CATG 6 cut(s) 9, 343, 538, 560, 776, 855
HspAI GCGC 3 cut(s) 114, 423, 581
Kpn2I TCCGGA 1 cut(s) 478
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 6 cut(s) 95, 323, 343, 347, 361, 775
LmnI GCTCC 4 cut(s) 23, 745, 844, 1195
Lsp1109I GCAGC 1 cut(s) 580
LweI GCATC 7 cut(s) 462, 484, 532, 679, 729, 867, 1035
MaeI CTAG 3 cut(s) 266, 1049, 1169
MaeII ACGT 4 cut(s) 37, 126, 485, 887
MaeIII GTNAC 4 cut(s) 26, 433, 635, 1163
MalI GATC 6 cut(s) 97, 325, 345, 349, 363, 777
MboI GATC 6 cut(s) 95, 323, 343, 347, 361, 775
MhlI GDGCHC 1 cut(s) 841
MlsI TGGCCA 1 cut(s) 1087
MluCI AATT 6 cut(s) 147, 218, 242, 407, 779, 1103
MluNI TGGCCA 1 cut(s) 1087
MlyI GAGTC 2 cut(s) 23, 754
Mox20I TGGCCA 1 cut(s) 1087
Mph1103I ATGCAT 1 cut(s) 477
MroI TCCGGA 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 793
MscI TGGCCA 1 cut(s) 1087
MseI TTAA 2 cut(s) 803, 1002
MslI CAYNNNNRTG 1 cut(s) 470
Msp20I TGGCCA 1 cut(s) 1087
MspI CCGG 2 cut(s) 479, 842
MspR9I CCNGG 1 cut(s) 460
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 1 cut(s) 460
MvnI CGCG 2 cut(s) 257, 470
MwoI GCNNNNNNNGC 4 cut(s) 627, 673, 682, 1133
NdeI CATATG 1 cut(s) 624
NdeII GATC 6 cut(s) 95, 323, 343, 347, 361, 775
NlaIII CATG 6 cut(s) 9, 343, 538, 560, 776, 855
NmeAIII GCCGAG 2 cut(s) 85, 718
NmuCI GTSAC 3 cut(s) 26, 433, 1163
NruI TCGCGA 1 cut(s) 470
NsiI ATGCAT 1 cut(s) 477
NspI RCATGY 2 cut(s) 538, 560
NspV TTCGAA 1 cut(s) 133
PaeI GCATGC 1 cut(s) 560
PciI ACATGT 1 cut(s) 534
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 57, 63
PctI GAATGC 1 cut(s) 667
PdmI GAANNNNTTC 1 cut(s) 793
PfeI GAWTC 5 cut(s) 152, 328, 899, 1018, 1097
PflMI CCANNNNNTGG 1 cut(s) 718
PkrI GCNGC 3 cut(s) 276, 357, 595
Ple19I CGATCG 1 cut(s) 326
PleI GAGTC 2 cut(s) 23, 754
PpsI GAGTC 2 cut(s) 23, 754
PscI ACATGT 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 458
PspFI CCCAGC 2 cut(s) 110, 546
PspGI CCWGG 1 cut(s) 458
PspPI GGNCC 1 cut(s) 1031
PstNI CAGNNNCTG 1 cut(s) 232
PvuI CGATCG 1 cut(s) 326
RruI TCGCGA 1 cut(s) 470
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
RseI CAYNNNNRTG 1 cut(s) 470
SaqAI TTAA 2 cut(s) 803, 1002
SatI GCNGC 3 cut(s) 275, 356, 594
Sau3AI GATC 6 cut(s) 95, 323, 343, 347, 361, 775
Sau96I GGNCC 1 cut(s) 1031
SchI GAGTC 2 cut(s) 23, 754
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 1 cut(s) 841
SfaNI GCATC 7 cut(s) 462, 484, 532, 679, 729, 867, 1035
SfuI TTCGAA 1 cut(s) 133
SinI GGWCC 1 cut(s) 1031
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 1 cut(s) 1221
SmoI CTYRAG 1 cut(s) 1221
SphI GCATGC 1 cut(s) 560
Sse9I AATT 6 cut(s) 147, 218, 242, 407, 779, 1103
SsiI CCGC 5 cut(s) 238, 272, 275, 287, 356
SspMI CTAG 3 cut(s) 266, 1049, 1169
StyD4I CCNGG 1 cut(s) 458
TaaI ACNGT 4 cut(s) 612, 812, 1027, 1210
TaiI ACGT 4 cut(s) 40, 129, 488, 890
TaqI TCGA 6 cut(s) 133, 322, 326, 346, 504, 1040
TasI AATT 6 cut(s) 147, 218, 242, 407, 779, 1103
TauI GCSGC 2 cut(s) 277, 358
TfiI GAWTC 5 cut(s) 152, 328, 899, 1018, 1097
Tru1I TTAA 2 cut(s) 803, 1002
Tru9I TTAA 2 cut(s) 803, 1002
TscAI CASTG 1 cut(s) 1168
TseFI GTSAC 3 cut(s) 26, 433, 1163
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 3 cut(s) 26, 433, 1163
TspDTI ATGAA 6 cut(s) 294, 399, 843, 912, 969, 1158
TspGWI ACGGA 3 cut(s) 285, 452, 606
TspRI CASTG 1 cut(s) 1168
Van91I CCANNNNNTGG 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 1031
XapI RAATTY 1 cut(s) 218
XceI RCATGY 2 cut(s) 538, 560
XmnI GAANNNNTTC 1 cut(s) 793
XspI CTAG 3 cut(s) 266, 1049, 1169
ZraI GACGTC 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.