FvH4_2g32800

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010000
Physical Location & Seq
Reverse (-)
24768608 .. 24771285
2678 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32800.t1

Sequence Viewer

Length: 1218 bp
ATGTCATGGCTCGCCCACTCGCTCTCTAAATCCCTCCGGCTCGACGACGACGACGACGCCGAAGATCACGACGTCGTCCGGCCCCACCCCAACCACAATGAAGCTCACGTCCCACCGACCACCATCGCCTCCACCAAATTACTCAATGAAATTGAATCCGACGCAAACGAACAAGAATTTGAAGACGCCCAAACGCGTGGCGTCAAGGACGACCTCTCGGAATTCCAGCAAACCCTAACGCGTCAATTCTGGGGCGTCGCCTCCTTCCTTGCTCCACCGCCACAGTCGTCTGATCGATTGGCTGATCCGCCCGATTGCGATCGATCCGAGCCGCCCGATCAACCGCCGATGGCGTCCAATGAAGACGAAGAAGAAGAAGAGGAGGAGGAGAGCGCGGTTGGTGTCACTGAGGAGGTGTTGAACTTCGCTAGAAACATCGCGATGCATCCAGAGACGTGGTTGGACTTTCCTCTTGATGAAGAAGAAGACTTGGGAGATTTTGAAATGTCTGATGCCCAGCAAGAGCATACTATGATAGTTGAACATCTTGCGCCGAGATTAGCTGCTTTGAGAATTGAGCTCTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTCGTTCTTCTGCATTCAAGACTCAACAAGCAGGATGCTGAGATTTTGTCCACACCTCAGGTAATGGAAGCCAGAGGGATGTGGATGCAAGAGCTACAATGTCAGACTCCATCAAGTTCTAAAATGTCAGAACATGAATGGTTTGGAAGAAGCACTTCACGTTTAAAAGAGAGTTCAACTACAATTCATGAAGAGTATGATGATTACTCTCCAGTCTCATCACATACTCCTCATTATGAGAATACACTTCATAGGACATTTCCTTTTGAATCAACCATGCCCTCCACAGCCTATGAGACTGAGAAGCACGCAGTTGAGAGTAATGATGTGCCATTCATTGATAAGTCTGTTATTGAAGAAAATCCCTCCATTAGGACTGAGGATAACAATGTGACAATTGGTCCATCCTCTCAGATACTACTTAATAGCTATGACGATGACGACGATGATGACTGGCCAGAGGATGATGATGAATTATATGGGCATAGTGGCACAGCTATTGCTTTGATAAATGAAGAAGACATATCTTTCAGTGATCTTGAGGATGATGATTTTCAGGCACCCTCCAAATTAAAGCTCAACCCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

406

Amino Acids

46.25

Weight (kDa)

4.22

Isoelectric Point (pI)

69.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 165 - 220 1.8e-12 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 1183
AccII CGCG 4 cut(s) 196, 241, 395, 440
AciI CCGC 5 cut(s) 278, 308, 332, 344, 395
AclWI GGATC 2 cut(s) 299, 318
AcoI YGGCCR 1 cut(s) 1079
AcsI RAATTY 2 cut(s) 176, 221
AcyI GRCGYC 6 cut(s) 57, 72, 186, 201, 255, 353
AfiI CCNNNNNNNGG 1 cut(s) 996
AflIII ACRYGT 2 cut(s) 194, 239
AgsI TTSAA 9 cut(s) 155, 182, 421, 503, 542, 642, 801, 893, 980
AhdI GACNNNNNGTC 1 cut(s) 1023
AjiI CACGTC 2 cut(s) 109, 456
AluBI AGCT 7 cut(s) 104, 563, 580, 718, 1053, 1121, 1201
AluI AGCT 7 cut(s) 104, 563, 580, 718, 1053, 1121, 1201
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 3 cut(s) 446, 844, 914
AlwI GGATC 2 cut(s) 299, 318
AoxI GGCC 2 cut(s) 80, 1079
ApeKI GCWGC 1 cut(s) 563
ApoI RAATTY 2 cut(s) 176, 221
Asp700I GAANNNNTTC 1 cut(s) 778
AspLEI GCGC 2 cut(s) 395, 553
AspS9I GGNCC 2 cut(s) 81, 1025
AvaII GGWCC 1 cut(s) 1025
AxyI CCTNAGG 1 cut(s) 681
BalI TGGCCA 1 cut(s) 1081
BanI GGYRCC 1 cut(s) 1183
BanII GRGCYC 1 cut(s) 582
BbsI GAAGAC 4 cut(s) 189, 369, 492, 1149
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 1 cut(s) 550
BccI CCATC 4 cut(s) 131, 343, 742, 1036
BcoDI GTCTC 3 cut(s) 446, 844, 914
BfaI CTAG 1 cut(s) 429
BisI GCNGC 2 cut(s) 332, 564
BlsI GCNGC 2 cut(s) 333, 565
Bme18I GGWCC 1 cut(s) 1025
BmeRI GACNNNNNGTC 1 cut(s) 1023
BmgBI CACGTC 2 cut(s) 109, 456
BmgT120I GGNCC 2 cut(s) 81, 1025
BmiI GGNNCC 2 cut(s) 83, 1185
BmsI GCATC 5 cut(s) 432, 454, 502, 649, 699
BpiI GAAGAC 4 cut(s) 189, 369, 492, 1149
BpmI CTGGAG 1 cut(s) 819
BpuEI CTTGAG 1 cut(s) 1184
Bsa29I ATCGAT 2 cut(s) 295, 322
BsaBI GATNNNNATC 1 cut(s) 318
BsaHI GRCGYC 6 cut(s) 57, 72, 186, 201, 255, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 996
Bse1I ACTGG 2 cut(s) 836, 1082
Bse21I CCTNAGG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 318
BseCI ATCGAT 2 cut(s) 295, 322
BseGI GGATG 7 cut(s) 445, 664, 708, 714, 1028, 1093, 1174
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 996
BseMII CTCAG 6 cut(s) 399, 654, 695, 915, 993, 1049
BseNI ACTGG 2 cut(s) 836, 1082
BseRI GAGGAG 5 cut(s) 395, 398, 401, 425, 843
BseXI GCAGC 1 cut(s) 550
BseYI CCCAGC 1 cut(s) 516
Bsh1236I CGCG 4 cut(s) 196, 241, 395, 440
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 82, 1081
BshNI GGYRCC 1 cut(s) 1183
BshVI ATCGAT 2 cut(s) 295, 322
BsiEI CGRYCG 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 582
BsiSI CCGG 2 cut(s) 37, 79
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 996
BsmAI GTCTC 3 cut(s) 446, 844, 914
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 95
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 2 cut(s) 82, 1081
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 7 cut(s) 64, 292, 304, 319, 323, 337, 1159
Bsp68I TCGCGA 1 cut(s) 440
BspACI CCGC 5 cut(s) 278, 308, 332, 344, 395
BspANI GGCC 2 cut(s) 82, 1081
BspCNI CTCAG 6 cut(s) 400, 655, 694, 916, 994, 1048
BspDI ATCGAT 2 cut(s) 295, 322
BspFNI CGCG 4 cut(s) 196, 241, 395, 440
BspHI TCATGA 1 cut(s) 811
BspLI GGNNCC 2 cut(s) 83, 1185
BspPI GGATC 2 cut(s) 299, 318
BspT107I GGYRCC 1 cut(s) 1183
BsrI ACTGG 2 cut(s) 836, 1082
BssMI GATC 7 cut(s) 64, 292, 304, 319, 323, 337, 1159
BssNI GRCGYC 6 cut(s) 57, 72, 186, 201, 255, 353
Bst4CI ACNGT 1 cut(s) 285
Bst6I CTCTTC 2 cut(s) 372, 810
BstACI GRCGYC 6 cut(s) 57, 72, 186, 201, 255, 353
BstC8I GCNNGC 2 cut(s) 12, 933
BstDEI CTNAG 6 cut(s) 408, 663, 681, 924, 1002, 1035
BstENI CCTNNNNNAGG 1 cut(s) 994
BstF5I GGATG 7 cut(s) 445, 664, 708, 714, 1028, 1093, 1174
BstFNI CGCG 4 cut(s) 196, 241, 395, 440
BstHHI GCGC 2 cut(s) 395, 553
BstKTI GATC 7 cut(s) 67, 295, 307, 322, 326, 340, 1162
BstMAI GTCTC 3 cut(s) 446, 844, 914
BstMBI GATC 7 cut(s) 64, 292, 304, 319, 323, 337, 1159
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 1 cut(s) 597
BstUI CGCG 4 cut(s) 196, 241, 395, 440
BstV1I GCAGC 1 cut(s) 550
BstV2I GAAGAC 4 cut(s) 189, 369, 492, 1149
BstXI CCANNNNNNTGG 2 cut(s) 197, 456
Bsu15I ATCGAT 2 cut(s) 295, 322
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 2 cut(s) 82, 1081
BsuTUI ATCGAT 2 cut(s) 295, 322
BtgZI GCGATG 3 cut(s) 109, 421, 455
BtrI CACGTC 2 cut(s) 109, 456
BtsCI GGATG 7 cut(s) 445, 664, 708, 714, 1028, 1093, 1174
BtsIMutI CAGTG 2 cut(s) 405, 1162
BtuMI TCGCGA 1 cut(s) 440
Cac8I GCNNGC 2 cut(s) 12, 933
CciI TCATGA 1 cut(s) 811
CfoI GCGC 2 cut(s) 395, 553
Cfr13I GGNCC 2 cut(s) 81, 1025
ClaI ATCGAT 2 cut(s) 295, 322
CseI GACGC 7 cut(s) 65, 170, 190, 194, 230, 244, 342
CviAII CATG 4 cut(s) 6, 758, 812, 901
DdeI CTNAG 6 cut(s) 408, 663, 681, 924, 1002, 1035
DpnI GATC 7 cut(s) 66, 294, 306, 321, 325, 339, 1161
DpnII GATC 7 cut(s) 64, 292, 304, 319, 323, 337, 1159
DraI TTTAAA 1 cut(s) 789
DriI GACNNNNNGTC 1 cut(s) 1023
EaeI YGGCCR 1 cut(s) 1079
Eam1104I CTCTTC 2 cut(s) 372, 810
Eam1105I GACNNNNNGTC 1 cut(s) 1023
EarI CTCTTC 2 cut(s) 372, 810
EciI GGCGGA 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 1025
Eco53kI GAGCTC 1 cut(s) 580
Eco81I CCTNAGG 1 cut(s) 681
EcoICRI GAGCTC 1 cut(s) 580
EcoNI CCTNNNNNAGG 1 cut(s) 994
EcoRI GAATTC 1 cut(s) 221
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 582
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 4 cut(s) 9, 761, 815, 904
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FaqI GGGAC 1 cut(s) 95
FatI CATG 4 cut(s) 5, 757, 811, 900
FauNDI CATATG 1 cut(s) 594
Fnu4HI GCNGC 2 cut(s) 332, 564
FokI GGATG 7 cut(s) 432, 671, 715, 721, 1015, 1100, 1181
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 2 cut(s) 332, 564
FspBI CTAG 1 cut(s) 429
GlaI GCGC 2 cut(s) 394, 552
GluI GCNGC 2 cut(s) 332, 564
GsaI CCCAGC 1 cut(s) 520
GsuI CTGGAG 1 cut(s) 819
HaeIII GGCC 2 cut(s) 82, 1081
HapII CCGG 2 cut(s) 37, 79
HgaI GACGC 7 cut(s) 65, 170, 190, 194, 230, 244, 342
HhaI GCGC 2 cut(s) 395, 553
Hin1I GRCGYC 6 cut(s) 57, 72, 186, 201, 255, 353
Hin1II CATG 4 cut(s) 9, 761, 815, 904
Hin6I GCGC 2 cut(s) 393, 551
HinP1I GCGC 2 cut(s) 393, 551
HinfI GANTC 4 cut(s) 155, 645, 730, 893
HpaII CCGG 2 cut(s) 37, 79
Hpy166II GTNNAC 1 cut(s) 675
Hpy188I TCNGA 8 cut(s) 160, 220, 292, 328, 511, 729, 754, 1038
Hpy188III TCNNGA 8 cut(s) 68, 439, 449, 473, 613, 642, 812, 1163
Hpy8I GTNNAC 1 cut(s) 675
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 285
HpyCH4IV ACGT 4 cut(s) 72, 108, 455, 784
HpyCH4V TGCA 3 cut(s) 445, 637, 712
HpyF10VI GCNNNNNNNGC 1 cut(s) 597
HpyF3I CTNAG 6 cut(s) 408, 663, 681, 924, 1002, 1035
HpySE526I ACGT 4 cut(s) 72, 108, 455, 784
Hsp92I GRCGYC 6 cut(s) 57, 72, 186, 201, 255, 353
Hsp92II CATG 4 cut(s) 9, 761, 815, 904
HspAI GCGC 2 cut(s) 393, 551
Kzo9I GATC 7 cut(s) 64, 292, 304, 319, 323, 337, 1159
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 5 cut(s) 432, 454, 502, 649, 699
MaeI CTAG 1 cut(s) 429
MaeII ACGT 4 cut(s) 72, 108, 455, 784
MaeIII GTNAC 3 cut(s) 403, 605, 1015
MalI GATC 7 cut(s) 66, 294, 306, 321, 325, 339, 1161
MboI GATC 7 cut(s) 64, 292, 304, 319, 323, 337, 1159
MfeI CAATTG 1 cut(s) 1020
MhlI GDGCHC 1 cut(s) 582
MlsI TGGCCA 1 cut(s) 1081
MluI ACGCGT 2 cut(s) 194, 239
MluNI TGGCCA 1 cut(s) 1081
MlyI GAGTC 2 cut(s) 639, 724
MmeI TCCRAC 2 cut(s) 183, 441
Mox20I TGGCCA 1 cut(s) 1081
Mph1103I ATGCAT 1 cut(s) 447
MroXI GAANNNNTTC 1 cut(s) 778
MscI TGGCCA 1 cut(s) 1081
MseI TTAA 3 cut(s) 788, 1047, 1196
MslI CAYNNNNRTG 1 cut(s) 440
Msp20I TGGCCA 1 cut(s) 1081
MspI CCGG 2 cut(s) 37, 79
MunI CAATTG 1 cut(s) 1020
Mva1269I GAATGC 1 cut(s) 637
MvnI CGCG 4 cut(s) 196, 241, 395, 440
MwoI GCNNNNNNNGC 1 cut(s) 597
NdeI CATATG 1 cut(s) 594
NdeII GATC 7 cut(s) 64, 292, 304, 319, 323, 337, 1159
NlaIII CATG 4 cut(s) 9, 761, 815, 904
NlaIV GGNNCC 2 cut(s) 83, 1185
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 2 cut(s) 403, 1015
NruI TCGCGA 1 cut(s) 440
NsiI ATGCAT 1 cut(s) 447
PagI TCATGA 1 cut(s) 811
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 51, 57, 1065
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 778
PfeI GAWTC 2 cut(s) 155, 893
PflFI GACNNNGTC 1 cut(s) 74
PkrI GCNGC 2 cut(s) 333, 565
Ple19I CGATCG 1 cut(s) 322
PleI GAGTC 2 cut(s) 639, 724
PpsI GAGTC 2 cut(s) 639, 724
Psp124BI GAGCTC 1 cut(s) 582
PspFI CCCAGC 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 83, 1185
PspPI GGNCC 2 cut(s) 81, 1025
PsyI GACNNNGTC 1 cut(s) 74
PvuI CGATCG 1 cut(s) 322
RruI TCGCGA 1 cut(s) 440
RseI CAYNNNNRTG 1 cut(s) 440
SacI GAGCTC 1 cut(s) 582
SaqAI TTAA 3 cut(s) 788, 1047, 1196
SatI GCNGC 2 cut(s) 332, 564
Sau3AI GATC 7 cut(s) 64, 292, 304, 319, 323, 337, 1159
Sau96I GGNCC 2 cut(s) 81, 1025
SchI GAGTC 2 cut(s) 639, 724
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 5 cut(s) 432, 454, 502, 649, 699
SinI GGWCC 1 cut(s) 1025
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
SsiI CCGC 5 cut(s) 278, 308, 332, 344, 395
SspMI CTAG 1 cut(s) 429
SstI GAGCTC 1 cut(s) 582
TaaI ACNGT 1 cut(s) 285
TaiI ACGT 4 cut(s) 75, 111, 458, 787
TaqI TCGA 3 cut(s) 42, 295, 322
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 2 cut(s) 155, 893
Tru1I TTAA 3 cut(s) 788, 1047, 1196
Tru9I TTAA 3 cut(s) 788, 1047, 1196
TscAI CASTG 2 cut(s) 412, 1162
TseFI GTSAC 2 cut(s) 403, 1015
TseI GCWGC 1 cut(s) 563
Tsp45I GTSAC 2 cut(s) 403, 1015
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 2 cut(s) 412, 1162
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 1025
XagI CCTNNNNNAGG 1 cut(s) 994
XapI RAATTY 2 cut(s) 176, 221
XmnI GAANNNNTTC 1 cut(s) 778
XspI CTAG 1 cut(s) 429
ZraI GACGTC 1 cut(s) 73
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.