pycom08g06580

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010000
Physical Location & Seq
Reverse (-)
5130075 .. 5132246
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06580.1

Sequence Viewer

Length: 1227 bp
ATGTCATGGCTCGCTCGCTCCCTCGGTGACTCTCTACGTCTCAACGACGACGGCGACGCCGTACCCCACGACAACGACAATGGATCACCAACCCCACCCAGCACTGACAACGACGTGGTTCGAAGAAGAAGCGAAATTGAATCTTCAGATGAGGAAAACGAAGACGAGGACGACCAAACCCGAGGCGTGAGGGAAGATTTGGATGAATTCAAGCAGACGCTGACCCGCCAATTCTGGGGCGTCGCGTCGTTCCTCGCTCCGCCGCCTCCCCATCCGCCACCTCCGTACCCTTCATTGCCGTCCAGTAATCGATCGGATCCTCCTGACATGGATCGATCCGAGCCGCCCGATCAATCGGCGATGGATTTTGATGAAGGTGAAGAGAAAGAGGAGGAATTGGAGGGGAATTGCGCCGTTGGGGTCACGGAGGAGGTATTGGCGTTCGCCAGGAACATCGCGATGCATCCGGAGACGTGGCTGGACTTTCCTCTTGACGAGGAGGAGGATTTGGGCGATTTTGACTTGTCTGATGCCCAGCAAGAGCATGCTATGGTGATTGAACATCTTGCGCCAAGATTTGCTGCTTTGAGATTTGAACTGTGTCCGTGCCATATGAGCAAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAATAAGCAGGATGCCGATGTCTTATCTACACCACAAGTAATGGAAGCCAGAGCAAAGTGGATGCAGGAGCTACATTGTCAGACTGCATTAGAACATGATCAATTTGGAAGAAACACTTCTTTTATAAAAGACAGTTCCAATATACTAGATGAAGATTGTGCTCTGGTCTCATCATGTAGTGTTTATTACGAGGACAAGATGCTAAGGATGTATCCTTATGAATCAAGGATGTCCCCCACGGCTTATGAGACTGAGAAGCACCCAGTTGAGAGTGATGAAGTCCCATTTATTGACAAGTCTGTTATTGAAGAAAAGCCCAACATTAAGAGCGAGGACAAGAATCTAACAGTTGGTCCATCCTCGAAGATGCTAGATAATATCTACAAAGATGATGACGATGATGACTGGCCAGAGGAAGATTCTGAATTAGATGGGTATAGTGGAACGGCTATTTACGCGATCAATGAAGAAGACATATCTTTTAGTGACCTAGAGGACGATGATTTCCTTGCTCCCACAAAACTCAAGACTGTCAAGAAACCTCATGGAAAGAAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

409

Amino Acids

46.45

Weight (kDa)

4.29

Isoelectric Point (pI)

70.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 171 - 226 1.5e-11 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 791
AccII CGCG 3 cut(s) 245, 458, 1124
AccIII TCCGGA 1 cut(s) 466
AciI CCGC 5 cut(s) 226, 260, 263, 275, 344
AclWI GGATC 5 cut(s) 91, 311, 324, 330, 339
AcoI YGGCCR 1 cut(s) 1073
AcsI RAATTY 1 cut(s) 206
AcuI CTGAAG 1 cut(s) 129
AcyI GRCGYC 2 cut(s) 57, 240
AfaI GTAC 2 cut(s) 63, 287
AfiI CCNNNNNNNGG 1 cut(s) 235
AgsI TTSAA 5 cut(s) 140, 211, 560, 596, 974
AjiI CACGTC 2 cut(s) 115, 474
AjnI CCWGG 1 cut(s) 446
AluBI AGCT 1 cut(s) 736
AluI AGCT 1 cut(s) 736
Alw21I GWGCWC 1 cut(s) 829
Alw26I GTCTC 4 cut(s) 44, 464, 838, 908
AlwI GGATC 5 cut(s) 91, 311, 324, 330, 339
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 180
Aor13HI TCCGGA 1 cut(s) 466
AoxI GGCC 1 cut(s) 1073
ApeKI GCWGC 1 cut(s) 581
ApoI RAATTY 1 cut(s) 206
Asp700I GAANNNNTTC 1 cut(s) 781
AspLEI GCGC 2 cut(s) 413, 571
AspS9I GGNCC 1 cut(s) 1019
AsuHPI GGTGA 4 cut(s) 38, 78, 389, 565
AsuII TTCGAA 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 1019
BaeI ACNNNNGTAYC 2 cut(s) 269, 302
BalI TGGCCA 1 cut(s) 1075
BamHI GGATCC 1 cut(s) 316
BbsI GAAGAC 2 cut(s) 168, 1143
Bbv12I GWGCWC 1 cut(s) 829
BbvI GCAGC 1 cut(s) 568
BccI CCATC 4 cut(s) 279, 355, 1030, 1091
BceAI ACGGC 6 cut(s) 44, 67, 283, 398, 921, 1128
BciT130I CCWGG 1 cut(s) 448
BciVI GTATCC 1 cut(s) 888
BclI TGATCA 1 cut(s) 763
BcoDI GTCTC 4 cut(s) 44, 464, 838, 908
BfaI CTAG 3 cut(s) 812, 1037, 1157
BfuI GTATCC 1 cut(s) 888
BisI GCNGC 3 cut(s) 263, 344, 582
BlsI GCNGC 3 cut(s) 264, 345, 583
Bme1390I CCNGG 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 1019
BmeT110I CYCGRG 1 cut(s) 180
BmgBI CACGTC 2 cut(s) 115, 474
BmgT120I GGNCC 1 cut(s) 1019
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 448
BmrI ACTGGG 1 cut(s) 923
BmsI GCATC 7 cut(s) 450, 472, 520, 667, 717, 855, 1023
BmuI ACTGGG 1 cut(s) 923
BpiI GAAGAC 2 cut(s) 168, 1143
Bpu10I CCTNAGC 1 cut(s) 869
Bpu14I TTCGAA 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 1175
Bsa29I ATCGAT 2 cut(s) 310, 334
BsaHI GRCGYC 2 cut(s) 57, 240
BsaI GGTCTC 1 cut(s) 838
BsaJI CCNNGG 3 cut(s) 22, 181, 903
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 3 cut(s) 303, 929, 1076
Bse3DI GCAATG 1 cut(s) 293
BseAI TCCGGA 1 cut(s) 466
BseBI CCWGG 1 cut(s) 448
BseCI ATCGAT 2 cut(s) 310, 334
BseDI CCNNGG 3 cut(s) 22, 181, 903
BseGI GGATG 8 cut(s) 208, 271, 463, 682, 732, 879, 900, 1022
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 909
BseNI ACTGG 3 cut(s) 303, 929, 1076
BseRI GAGGAG 4 cut(s) 404, 443, 512, 515
BseXI GCAGC 1 cut(s) 568
BseYI CCCAGC 2 cut(s) 98, 534
Bsh1236I CGCG 3 cut(s) 245, 458, 1124
Bsh1285I CGRYCG 1 cut(s) 314
BshFI GGCC 1 cut(s) 1075
BshVI ATCGAT 2 cut(s) 310, 334
BsiEI CGRYCG 1 cut(s) 314
BsiHKAI GWGCWC 1 cut(s) 829
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 1 cut(s) 467
BslFI GGGAC 2 cut(s) 883, 932
BslI CCNNNNNNNGG 1 cut(s) 235
BsmAI GTCTC 4 cut(s) 44, 464, 838, 908
BsmBI CGTCTC 2 cut(s) 44, 464
BsmFI GGGAC 2 cut(s) 883, 932
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 1 cut(s) 1075
Bso31I GGTCTC 1 cut(s) 838
BsoBI CYCGRG 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 829
Bsp13I TCCGGA 1 cut(s) 466
Bsp143I GATC 8 cut(s) 83, 311, 316, 331, 335, 349, 763, 1125
Bsp68I TCGCGA 1 cut(s) 458
BspACI CCGC 5 cut(s) 226, 260, 263, 275, 344
BspANI GGCC 1 cut(s) 1075
BspCNI CTCAG 1 cut(s) 910
BspDI ATCGAT 2 cut(s) 310, 334
BspEI TCCGGA 1 cut(s) 466
BspFNI CGCG 3 cut(s) 245, 458, 1124
BspLI GGNNCC 1 cut(s) 318
BspPI GGATC 5 cut(s) 91, 311, 324, 330, 339
BspT104I TTCGAA 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 838
BsrDI GCAATG 1 cut(s) 293
BsrI ACTGG 3 cut(s) 303, 929, 1076
BssECI CCNNGG 3 cut(s) 22, 181, 903
BssMI GATC 8 cut(s) 83, 311, 316, 331, 335, 349, 763, 1125
BssNI GRCGYC 2 cut(s) 57, 240
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 4 cut(s) 600, 800, 1015, 1198
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 2 cut(s) 57, 240
BstBI TTCGAA 1 cut(s) 121
BstC8I GCNNGC 3 cut(s) 12, 16, 546
BstDEI CTNAG 2 cut(s) 869, 918
BstDSI CCRYGG 1 cut(s) 903
BstF5I GGATG 8 cut(s) 208, 271, 463, 682, 732, 879, 900, 1022
BstFNI CGCG 3 cut(s) 245, 458, 1124
BstHHI GCGC 2 cut(s) 413, 571
BstKTI GATC 8 cut(s) 86, 314, 319, 334, 338, 352, 766, 1128
BstMAI GTCTC 4 cut(s) 44, 464, 838, 908
BstMBI GATC 8 cut(s) 83, 311, 316, 331, 335, 349, 763, 1125
BstMCI CGRYCG 1 cut(s) 314
BstMWI GCNNNNNNNGC 4 cut(s) 615, 661, 670, 1121
BstNI CCWGG 1 cut(s) 448
BstNSI RCATGY 1 cut(s) 548
BstSCI CCNGG 1 cut(s) 446
BstUI CGCG 3 cut(s) 245, 458, 1124
BstV1I GCAGC 1 cut(s) 568
BstV2I GAAGAC 2 cut(s) 168, 1143
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
Bsu15I ATCGAT 2 cut(s) 310, 334
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 1 cut(s) 1075
BsuTUI ATCGAT 2 cut(s) 310, 334
BtgI CCRYGG 1 cut(s) 903
BtgZI GCGATG 3 cut(s) 374, 439, 473
BtrI CACGTC 2 cut(s) 115, 474
BtsCI GGATG 8 cut(s) 208, 271, 463, 682, 732, 879, 900, 1022
BtsIMutI CAGTG 1 cut(s) 102
BtuMI TCGCGA 1 cut(s) 458
Cac8I GCNNGC 3 cut(s) 12, 16, 546
CaiI CAGNNNCTG 1 cut(s) 220
CfoI GCGC 2 cut(s) 413, 571
Cfr13I GGNCC 1 cut(s) 1019
ClaI ATCGAT 2 cut(s) 310, 334
CseI GACGC 4 cut(s) 65, 226, 229, 234
Csp6I GTAC 2 cut(s) 62, 286
CviAII CATG 6 cut(s) 6, 328, 545, 761, 840, 1211
CviQI GTAC 2 cut(s) 62, 286
DdeI CTNAG 2 cut(s) 869, 918
DpnI GATC 8 cut(s) 85, 313, 318, 333, 337, 351, 765, 1127
DpnII GATC 8 cut(s) 83, 311, 316, 331, 335, 349, 763, 1125
EaeI YGGCCR 1 cut(s) 1073
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
EciI GGCGGA 2 cut(s) 249, 264
Eco31I GGTCTC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 1019
Eco57I CTGAAG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 180
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 446
EcoT22I ATGCAT 1 cut(s) 465
Esp3I CGTCTC 2 cut(s) 44, 464
FaeI CATG 6 cut(s) 9, 331, 548, 764, 843, 1214
FalI AAGNNNNNCTT 4 cut(s) 611, 643, 766, 798
FaqI GGGAC 2 cut(s) 883, 932
FatI CATG 6 cut(s) 5, 327, 544, 760, 839, 1210
FauI CCCGC 1 cut(s) 233
FauNDI CATATG 1 cut(s) 612
FbaI TGATCA 1 cut(s) 763
Fnu4HI GCNGC 3 cut(s) 263, 344, 582
FokI GGATG 8 cut(s) 215, 258, 450, 689, 739, 886, 907, 1009
Fsp4HI GCNGC 3 cut(s) 263, 344, 582
FspBI CTAG 3 cut(s) 812, 1037, 1157
GlaI GCGC 2 cut(s) 412, 570
GluI GCNGC 3 cut(s) 263, 344, 582
GsaI CCCAGC 2 cut(s) 102, 538
HaeIII GGCC 1 cut(s) 1075
HapII CCGG 1 cut(s) 467
HgaI GACGC 4 cut(s) 65, 226, 229, 234
HhaI GCGC 2 cut(s) 413, 571
Hin1I GRCGYC 2 cut(s) 57, 240
Hin1II CATG 6 cut(s) 9, 331, 548, 764, 843, 1214
Hin6I GCGC 2 cut(s) 411, 569
HinP1I GCGC 2 cut(s) 411, 569
HinfI GANTC 5 cut(s) 29, 140, 887, 1006, 1085
HpaII CCGG 1 cut(s) 467
HphI GGTGA 4 cut(s) 38, 78, 389, 565
Hpy188I TCNGA 6 cut(s) 148, 316, 340, 529, 747, 1090
Hpy188III TCNNGA 7 cut(s) 323, 457, 467, 491, 631, 1192, 1201
Hpy99I CGWCG 6 cut(s) 50, 53, 59, 116, 245, 250
HpyAV CCTTC 2 cut(s) 300, 368
HpyCH4III ACNGT 4 cut(s) 600, 800, 1015, 1198
HpyCH4IV ACGT 3 cut(s) 37, 114, 473
HpyCH4V TGCA 4 cut(s) 463, 655, 730, 752
HpyF10VI GCNNNNNNNGC 4 cut(s) 615, 661, 670, 1121
HpyF3I CTNAG 2 cut(s) 869, 918
HpySE526I ACGT 3 cut(s) 37, 114, 473
Hsp92I GRCGYC 2 cut(s) 57, 240
Hsp92II CATG 6 cut(s) 9, 331, 548, 764, 843, 1214
HspAI GCGC 2 cut(s) 411, 569
Kpn2I TCCGGA 1 cut(s) 466
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 8 cut(s) 83, 311, 316, 331, 335, 349, 763, 1125
LmnI GCTCC 4 cut(s) 23, 262, 733, 1183
Lsp1109I GCAGC 1 cut(s) 568
LweI GCATC 7 cut(s) 450, 472, 520, 667, 717, 855, 1023
MaeI CTAG 3 cut(s) 812, 1037, 1157
MaeII ACGT 3 cut(s) 37, 114, 473
MaeIII GTNAC 4 cut(s) 26, 421, 623, 1151
MalI GATC 8 cut(s) 85, 313, 318, 333, 337, 351, 765, 1127
MboI GATC 8 cut(s) 83, 311, 316, 331, 335, 349, 763, 1125
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 829
MlsI TGGCCA 1 cut(s) 1075
MluCI AATT 7 cut(s) 135, 206, 230, 395, 406, 767, 1091
MluNI TGGCCA 1 cut(s) 1075
MlyI GAGTC 1 cut(s) 23
Mox20I TGGCCA 1 cut(s) 1075
Mph1103I ATGCAT 1 cut(s) 465
MroI TCCGGA 1 cut(s) 466
MroXI GAANNNNTTC 1 cut(s) 781
MscI TGGCCA 1 cut(s) 1075
MseI TTAA 2 cut(s) 990, 1225
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 1075
MspI CCGG 1 cut(s) 467
MspR9I CCNGG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 1 cut(s) 448
MvnI CGCG 3 cut(s) 245, 458, 1124
MwoI GCNNNNNNNGC 4 cut(s) 615, 661, 670, 1121
NdeI CATATG 1 cut(s) 612
NdeII GATC 8 cut(s) 83, 311, 316, 331, 335, 349, 763, 1125
NlaIII CATG 6 cut(s) 9, 331, 548, 764, 843, 1214
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 3 cut(s) 26, 421, 1151
NruI TCGCGA 1 cut(s) 458
NsiI ATGCAT 1 cut(s) 465
NspI RCATGY 1 cut(s) 548
NspV TTCGAA 1 cut(s) 121
PaeI GCATGC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 57
PctI GAATGC 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 781
PfeI GAWTC 4 cut(s) 140, 887, 1006, 1085
PkrI GCNGC 3 cut(s) 264, 345, 583
Ple19I CGATCG 1 cut(s) 314
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PsiI TTATAA 1 cut(s) 791
Psp6I CCWGG 1 cut(s) 446
PspFI CCCAGC 2 cut(s) 98, 534
PspGI CCWGG 1 cut(s) 446
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 1 cut(s) 1019
PstNI CAGNNNCTG 1 cut(s) 220
PsuI RGATCY 1 cut(s) 316
PvuI CGATCG 1 cut(s) 314
RruI TCGCGA 1 cut(s) 458
RsaI GTAC 2 cut(s) 63, 287
RsaNI GTAC 2 cut(s) 62, 286
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 2 cut(s) 990, 1225
SatI GCNGC 3 cut(s) 263, 344, 582
Sau3AI GATC 8 cut(s) 83, 311, 316, 331, 335, 349, 763, 1125
Sau96I GGNCC 1 cut(s) 1019
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 1 cut(s) 829
SfaNI GCATC 7 cut(s) 450, 472, 520, 667, 717, 855, 1023
SfuI TTCGAA 1 cut(s) 121
SinI GGWCC 1 cut(s) 1019
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 1190
SmoI CTYRAG 1 cut(s) 1190
SphI GCATGC 1 cut(s) 548
Sse9I AATT 7 cut(s) 135, 206, 230, 395, 406, 767, 1091
SsiI CCGC 5 cut(s) 226, 260, 263, 275, 344
SspMI CTAG 3 cut(s) 812, 1037, 1157
StyD4I CCNGG 1 cut(s) 446
TaaI ACNGT 4 cut(s) 600, 800, 1015, 1198
TaiI ACGT 3 cut(s) 40, 117, 476
TaqI TCGA 5 cut(s) 121, 310, 334, 659, 1028
TasI AATT 7 cut(s) 135, 206, 230, 395, 406, 767, 1091
TauI GCSGC 2 cut(s) 265, 346
TfiI GAWTC 4 cut(s) 140, 887, 1006, 1085
Tru1I TTAA 2 cut(s) 990, 1225
Tru9I TTAA 2 cut(s) 990, 1225
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 3 cut(s) 26, 421, 1151
TseI GCWGC 1 cut(s) 581
Tsp45I GTSAC 3 cut(s) 26, 421, 1151
TspDTI ATGAA 7 cut(s) 219, 282, 387, 831, 900, 957, 1146
TspGWI ACGGA 3 cut(s) 273, 440, 594
TspRI CASTG 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 1019
XapI RAATTY 1 cut(s) 206
XceI RCATGY 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 781
XspI CTAG 3 cut(s) 812, 1037, 1157
Zsp2I ATGCAT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.