Rw6G042610

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010000
Physical Location & Seq
Forward (+)
66009922 .. 66012601
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G042610.1

Sequence Viewer

Length: 1227 bp
ATGTCATGGCTCGCCCACTCGCTCTCTAAATCCCTCCGAATCGACGGCGACGACGGCGAAGGCCAAGATCACGACGACGACGTCGTACCCCACCACCACAACAACAATGAAGCTGACGTCCCGCCCACCGCCTCCACCAAACCCCAAGACAACGAAATCCAATCGGATGCAGAAGCAGTTGAAGACGCCCAAACGCGTGGCGTGAAAGAAGACCTATCGGAATTCCAGCAAACCCTGACGCGCCAATTCTGGGGCGTCGCCTCGTTTCTTGCGCCACCACCGCCCTCTGATCGATTAGCTTCTCCACCCGATTGCGATCGATCCGAGCCGGCCGATCACCCGCCCGGTCAATCGCCGATGGCGTTGAATGAAGACGAAGAAGAGGAGGAGGAGGAGTGCGCCGTCGGTGTCACTGACGAGGTCTTGGCCTTCGCTAGAAACATCGCGATGCATCCAGAGACCTGGTTGGACTTTCCTCTTGATGAAGAAGAAGACTTGGGAGATTTTGAAATGTCTGATGCCCAGCAAGAGCATGCTATGATGATTGAACATCTTGCGCCGAGATTAGCTGCTTTGAGAATCGAGCTTTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAACAAGCAGGATGCTGAGGTTTTGTCCACACCTCAGGTAATGGAAGCCAGAGCAATGTGGATGCAAGAGCTACAATGTCAGACTCCAACATCAATTTCTAAAATGTCAGAACATGAATGGTTTGGAAGAAGCACTTCACATTTAAAATACAGTTCAAATGCAATACATGAAGATTATGAAGATTACTCTCCGGTCTCATCACATACTCCTCATTATGAGGATACACTTCATAAGACGTATCCTTTTGAATCAACCATGCCTTCCACAGCCGATGAGACTGAGAAGCACCCAGTTGAGAGTAATGAGATGCCATTCATTGATAAGTCCGTTATCGAAGAAAAGCCCTTCATTAGGACTGAGGATAAGAATGTGACAGTTGGTCCATCCTCTGAGATACTAGTTAATAGCTATGACGACGACGACGATGATGACTGGCCAGAGGATGATGGTGAATTATATGGGTGTAGTGGAACAGCTGTTGCTTTGATAAATGAAGAAGACATATCTTTTAGTGATCTTGAGGATGATGATTTCCAGGCACCCTCTAAATTAAAGTTCATCTCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

408

Amino Acids

46.2

Weight (kDa)

4.24

Isoelectric Point (pI)

69.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 167 - 222 1.7e-13 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AatII GACGTC 2 cut(s) 84, 120
AccB1I GGYRCC 1 cut(s) 1192
AccII CGCG 3 cut(s) 196, 241, 446
AciI CCGC 4 cut(s) 122, 129, 281, 341
AclWI GGATC 1 cut(s) 315
AcoI YGGCCR 2 cut(s) 330, 1088
AcsI RAATTY 1 cut(s) 221
AcyI GRCGYC 4 cut(s) 81, 117, 186, 255
AfaI GTAC 1 cut(s) 87
AfiI CCNNNNNNNGG 2 cut(s) 250, 1005
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 6 cut(s) 182, 367, 509, 548, 810, 902
AhdI GACNNNNNGTC 1 cut(s) 1032
AhlI ACTAGT 1 cut(s) 1051
AjnI CCWGG 2 cut(s) 461, 1188
AjuI GAANNNNNNNTTGG 2 cut(s) 733, 765
AluBI AGCT 7 cut(s) 113, 299, 569, 586, 724, 1062, 1130
AluI AGCT 7 cut(s) 113, 299, 569, 586, 724, 1062, 1130
Alw26I GTCTC 3 cut(s) 452, 853, 923
AlwI GGATC 1 cut(s) 315
AoxI GGCC 4 cut(s) 61, 330, 426, 1088
ApeKI GCWGC 1 cut(s) 569
ApoI RAATTY 1 cut(s) 221
Asp700I GAANNNNTTC 1 cut(s) 787
AspLEI GCGC 4 cut(s) 243, 274, 401, 559
AspS9I GGNCC 1 cut(s) 1034
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 329, 1115
AvaII GGWCC 1 cut(s) 1034
AxyI CCTNAGG 1 cut(s) 687
BalI TGGCCA 1 cut(s) 1090
BanI GGYRCC 1 cut(s) 1192
BbsI GAAGAC 5 cut(s) 189, 216, 378, 498, 1158
BbvCI CCTCAGC 1 cut(s) 669
BbvI GCAGC 1 cut(s) 556
BccI CCATC 3 cut(s) 352, 1045, 1094
BceAI ACGGC 3 cut(s) 61, 70, 386
BciT130I CCWGG 2 cut(s) 463, 1190
BciVI GTATCC 2 cut(s) 868, 903
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 3 cut(s) 452, 853, 923
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 2 cut(s) 435, 1052
BfuI GTATCC 2 cut(s) 868, 903
BisI GCNGC 1 cut(s) 570
BlsI GCNGC 1 cut(s) 571
Bme1390I CCNGG 3 cut(s) 345, 463, 1190
Bme18I GGWCC 1 cut(s) 1034
BmeRI GACNNNNNGTC 1 cut(s) 1032
BmgT120I GGNCC 1 cut(s) 1034
BmiI GGNNCC 1 cut(s) 1194
BmrFI CCNGG 3 cut(s) 345, 463, 1190
BmrI ACTGGG 1 cut(s) 938
BmsI GCATC 7 cut(s) 157, 438, 460, 508, 655, 705, 951
BmuI ACTGGG 1 cut(s) 938
BpiI GAAGAC 5 cut(s) 189, 216, 378, 498, 1158
Bpu10I CCTNAGC 1 cut(s) 669
BpuEI CTTGAG 1 cut(s) 1193
BpuMI CCSGG 1 cut(s) 345
Bsa29I ATCGAT 2 cut(s) 292, 319
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 4 cut(s) 81, 117, 186, 255
BsaI GGTCTC 2 cut(s) 452, 853
BsaWI WCCGGW 1 cut(s) 844
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 1005
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 2 cut(s) 944, 1091
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 1 cut(s) 714
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 2 cut(s) 463, 1190
BseCI ATCGAT 2 cut(s) 292, 319
BseGI GGATG 7 cut(s) 172, 451, 670, 720, 1037, 1102, 1183
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 250, 1005
BseMI GCAATG 1 cut(s) 714
BseMII CTCAG 5 cut(s) 660, 701, 924, 1002, 1035
BseNI ACTGG 2 cut(s) 944, 1091
BseRI GAGGAG 5 cut(s) 398, 401, 404, 407, 852
BseX3I CGGCCG 1 cut(s) 330
BseXI GCAGC 1 cut(s) 556
BseYI CCCAGC 1 cut(s) 522
Bsh1236I CGCG 3 cut(s) 196, 241, 446
Bsh1285I CGRYCG 2 cut(s) 319, 333
BshFI GGCC 4 cut(s) 63, 332, 428, 1090
BshNI GGYRCC 1 cut(s) 1192
BshVI ATCGAT 2 cut(s) 292, 319
BsiEI CGRYCG 2 cut(s) 319, 333
BsiSI CCGG 3 cut(s) 329, 345, 845
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 2 cut(s) 250, 1005
BsmAI GTCTC 3 cut(s) 452, 853, 923
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 643
BsnI GGCC 4 cut(s) 63, 332, 428, 1090
Bso31I GGTCTC 2 cut(s) 452, 853
Bsp143I GATC 6 cut(s) 67, 289, 316, 320, 334, 1168
Bsp68I TCGCGA 1 cut(s) 446
BspACI CCGC 4 cut(s) 122, 129, 281, 341
BspANI GGCC 4 cut(s) 63, 332, 428, 1090
BspCNI CTCAG 5 cut(s) 661, 700, 925, 1003, 1036
BspDI ATCGAT 2 cut(s) 292, 319
BspFNI CGCG 3 cut(s) 196, 241, 446
BspLI GGNNCC 1 cut(s) 1194
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 1192
BspTNI GGTCTC 2 cut(s) 452, 853
BsrDI GCAATG 1 cut(s) 714
BsrFI RCCGGY 1 cut(s) 328
BsrI ACTGG 2 cut(s) 944, 1091
BssAI RCCGGY 1 cut(s) 328
BssMI GATC 6 cut(s) 67, 289, 316, 320, 334, 1168
BssNI GRCGYC 4 cut(s) 81, 117, 186, 255
Bst2UI CCWGG 2 cut(s) 463, 1190
Bst4CI ACNGT 2 cut(s) 806, 1030
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 4 cut(s) 81, 117, 186, 255
BstC8I GCNNGC 3 cut(s) 12, 330, 534
BstDEI CTNAG 5 cut(s) 669, 687, 933, 1011, 1044
BstENI CCTNNNNNAGG 1 cut(s) 1003
BstF5I GGATG 7 cut(s) 172, 451, 670, 720, 1037, 1102, 1183
BstFNI CGCG 3 cut(s) 196, 241, 446
BstHHI GCGC 4 cut(s) 243, 274, 401, 559
BstKTI GATC 6 cut(s) 70, 292, 319, 323, 337, 1171
BstMAI GTCTC 3 cut(s) 452, 853, 923
BstMBI GATC 6 cut(s) 67, 289, 316, 320, 334, 1168
BstMCI CGRYCG 2 cut(s) 319, 333
BstMWI GCNNNNNNNGC 5 cut(s) 54, 280, 603, 649, 658
BstNI CCWGG 2 cut(s) 463, 1190
BstNSI RCATGY 1 cut(s) 536
BstSCI CCNGG 3 cut(s) 343, 461, 1188
BstUI CGCG 3 cut(s) 196, 241, 446
BstV1I GCAGC 1 cut(s) 556
BstV2I GAAGAC 5 cut(s) 189, 216, 378, 498, 1158
BstXI CCANNNNNNTGG 2 cut(s) 197, 462
BstZI CGGCCG 1 cut(s) 330
Bsu15I ATCGAT 2 cut(s) 292, 319
Bsu36I CCTNAGG 1 cut(s) 687
BsuI GTATCC 2 cut(s) 868, 903
BsuRI GGCC 4 cut(s) 63, 332, 428, 1090
BsuTUI ATCGAT 2 cut(s) 292, 319
BtgZI GCGATG 2 cut(s) 427, 461
BtsCI GGATG 7 cut(s) 172, 451, 670, 720, 1037, 1102, 1183
BtsIMutI CAGTG 1 cut(s) 411
BtuMI TCGCGA 1 cut(s) 446
Cac8I GCNNGC 3 cut(s) 12, 330, 534
CfoI GCGC 4 cut(s) 243, 274, 401, 559
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 1 cut(s) 1034
ClaI ATCGAT 2 cut(s) 292, 319
CseI GACGC 3 cut(s) 194, 244, 247
CsiI ACCWGGT 1 cut(s) 461
Csp6I GTAC 1 cut(s) 86
CviAII CATG 5 cut(s) 6, 533, 767, 821, 910
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 5 cut(s) 669, 687, 933, 1011, 1044
DpnI GATC 6 cut(s) 69, 291, 318, 322, 336, 1170
DpnII GATC 6 cut(s) 67, 289, 316, 320, 334, 1168
DraI TTTAAA 1 cut(s) 798
DrdI GACNNNNNNGTC 1 cut(s) 80
DriI GACNNNNNGTC 1 cut(s) 1032
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 2 cut(s) 330, 1088
EagI CGGCCG 1 cut(s) 330
Eam1104I CTCTTC 1 cut(s) 375
Eam1105I GACNNNNNGTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 375
EclXI CGGCCG 1 cut(s) 330
Eco31I GGTCTC 2 cut(s) 452, 853
Eco47I GGWCC 1 cut(s) 1034
Eco52I CGGCCG 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 687
EcoNI CCTNNNNNAGG 1 cut(s) 1003
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 461, 1188
EcoT22I ATGCAT 1 cut(s) 453
FaeI CATG 5 cut(s) 9, 536, 770, 824, 913
FalI AAGNNNNNCTT 2 cut(s) 772, 804
FaqI GGGAC 1 cut(s) 104
FatI CATG 5 cut(s) 5, 532, 766, 820, 909
FauI CCCGC 2 cut(s) 129, 348
FauNDI CATATG 1 cut(s) 600
Fnu4HI GCNGC 1 cut(s) 570
FokI GGATG 7 cut(s) 179, 438, 677, 727, 1024, 1109, 1190
Fsp4HI GCNGC 1 cut(s) 570
FspBI CTAG 2 cut(s) 435, 1052
GlaI GCGC 4 cut(s) 242, 273, 400, 558
GluI GCNGC 1 cut(s) 570
GsaI CCCAGC 1 cut(s) 526
HaeIII GGCC 4 cut(s) 63, 332, 428, 1090
HapII CCGG 3 cut(s) 329, 345, 845
HgaI GACGC 3 cut(s) 194, 244, 247
HhaI GCGC 4 cut(s) 243, 274, 401, 559
Hin1I GRCGYC 4 cut(s) 81, 117, 186, 255
Hin1II CATG 5 cut(s) 9, 536, 770, 824, 913
Hin6I GCGC 4 cut(s) 241, 272, 399, 557
HinP1I GCGC 4 cut(s) 241, 272, 399, 557
HinfI GANTC 4 cut(s) 39, 579, 736, 902
HpaII CCGG 3 cut(s) 329, 345, 845
HphI GGTGA 2 cut(s) 329, 1115
Hpy166II GTNNAC 1 cut(s) 681
Hpy188I TCNGA 9 cut(s) 38, 166, 220, 289, 325, 517, 735, 763, 1045
Hpy188III TCNNGA 6 cut(s) 71, 445, 455, 479, 619, 1172
Hpy8I GTNNAC 1 cut(s) 681
HpyAV CCTTC 4 cut(s) 53, 439, 924, 1009
HpyCH4III ACNGT 2 cut(s) 806, 1030
HpyCH4IV ACGT 3 cut(s) 81, 117, 890
HpyCH4V TGCA 5 cut(s) 170, 451, 643, 718, 815
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 280, 603, 649, 658
HpyF3I CTNAG 5 cut(s) 669, 687, 933, 1011, 1044
HpySE526I ACGT 3 cut(s) 81, 117, 890
Hsp92I GRCGYC 4 cut(s) 81, 117, 186, 255
Hsp92II CATG 5 cut(s) 9, 536, 770, 824, 913
HspAI GCGC 4 cut(s) 241, 272, 399, 557
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Kzo9I GATC 6 cut(s) 67, 289, 316, 320, 334, 1168
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 7 cut(s) 157, 438, 460, 508, 655, 705, 951
MabI ACCWGGT 1 cut(s) 461
MaeI CTAG 2 cut(s) 435, 1052
MaeII ACGT 3 cut(s) 81, 117, 890
MaeIII GTNAC 3 cut(s) 409, 611, 1024
MalI GATC 6 cut(s) 69, 291, 318, 322, 336, 1170
MboI GATC 6 cut(s) 67, 289, 316, 320, 334, 1168
MlsI TGGCCA 1 cut(s) 1090
MluCI AATT 5 cut(s) 221, 245, 747, 1106, 1202
MluI ACGCGT 1 cut(s) 194
MluNI TGGCCA 1 cut(s) 1090
MlyI GAGTC 1 cut(s) 730
MmeI TCCRAC 2 cut(s) 447, 764
Mox20I TGGCCA 1 cut(s) 1090
Mph1103I ATGCAT 1 cut(s) 453
MroNI GCCGGC 1 cut(s) 328
MroXI GAANNNNTTC 1 cut(s) 787
MscI TGGCCA 1 cut(s) 1090
MseI TTAA 3 cut(s) 797, 1056, 1205
MslI CAYNNNNRTG 1 cut(s) 446
Msp20I TGGCCA 1 cut(s) 1090
MspA1I CMGCKG 1 cut(s) 1130
MspI CCGG 3 cut(s) 329, 345, 845
MspR9I CCNGG 3 cut(s) 345, 463, 1190
Mva1269I GAATGC 1 cut(s) 643
MvaI CCWGG 2 cut(s) 463, 1190
MvnI CGCG 3 cut(s) 196, 241, 446
MwoI GCNNNNNNNGC 5 cut(s) 54, 280, 603, 649, 658
NaeI GCCGGC 1 cut(s) 330
NciI CCSGG 1 cut(s) 345
NdeI CATATG 1 cut(s) 600
NdeII GATC 6 cut(s) 67, 289, 316, 320, 334, 1168
NgoMIV GCCGGC 1 cut(s) 328
NlaIII CATG 5 cut(s) 9, 536, 770, 824, 913
NlaIV GGNNCC 1 cut(s) 1194
NmeAIII GCCGAG 1 cut(s) 585
NmuCI GTSAC 2 cut(s) 409, 1024
NruI TCGCGA 1 cut(s) 446
NsiI ATGCAT 1 cut(s) 453
NspI RCATGY 1 cut(s) 536
PaeI GCATGC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 5 cut(s) 48, 78, 81, 359, 1074
PctI GAATGC 1 cut(s) 643
PdiI GCCGGC 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 787
PfeI GAWTC 3 cut(s) 39, 579, 902
PflFI GACNNNGTC 2 cut(s) 80, 419
PkrI GCNGC 1 cut(s) 571
Ple19I CGATCG 1 cut(s) 319
PleI GAGTC 1 cut(s) 730
PpsI GAGTC 1 cut(s) 730
Psp6I CCWGG 2 cut(s) 461, 1188
PspFI CCCAGC 1 cut(s) 522
PspGI CCWGG 2 cut(s) 461, 1188
PspN4I GGNNCC 1 cut(s) 1194
PspPI GGNCC 1 cut(s) 1034
PsyI GACNNNGTC 2 cut(s) 80, 419
PvuI CGATCG 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 1130
RruI TCGCGA 1 cut(s) 446
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 3 cut(s) 797, 1056, 1205
SatI GCNGC 1 cut(s) 570
Sau3AI GATC 6 cut(s) 67, 289, 316, 320, 334, 1168
Sau96I GGNCC 1 cut(s) 1034
SchI GAGTC 1 cut(s) 730
ScrFI CCNGG 3 cut(s) 345, 463, 1190
SexAI ACCWGGT 1 cut(s) 461
SfaNI GCATC 7 cut(s) 157, 438, 460, 508, 655, 705, 951
SinI GGWCC 1 cut(s) 1034
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 1172
SmoI CTYRAG 1 cut(s) 1172
SpeI ACTAGT 1 cut(s) 1051
SphI GCATGC 1 cut(s) 536
Sse9I AATT 5 cut(s) 221, 245, 747, 1106, 1202
SsiI CCGC 4 cut(s) 122, 129, 281, 341
SspMI CTAG 2 cut(s) 435, 1052
StyD4I CCNGG 3 cut(s) 343, 461, 1188
TaaI ACNGT 2 cut(s) 806, 1030
TaiI ACGT 3 cut(s) 84, 120, 893
TaqI TCGA 6 cut(s) 42, 292, 319, 582, 647, 987
TasI AATT 5 cut(s) 221, 245, 747, 1106, 1202
TfiI GAWTC 3 cut(s) 39, 579, 902
Tru1I TTAA 3 cut(s) 797, 1056, 1205
Tru9I TTAA 3 cut(s) 797, 1056, 1205
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 2 cut(s) 409, 1024
TseI GCWGC 1 cut(s) 569
Tsp45I GTSAC 2 cut(s) 409, 1024
TspGWI ACGGA 2 cut(s) 582, 970
TspRI CASTG 1 cut(s) 418
Tth111I GACNNNGTC 2 cut(s) 80, 419
VpaK11BI GGWCC 1 cut(s) 1034
XagI CCTNNNNNAGG 1 cut(s) 1003
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 787
XspI CTAG 2 cut(s) 435, 1052
ZraI GACGTC 2 cut(s) 82, 118
Zsp2I ATGCAT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.