Rmu_sc0009846.1_g000003

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009846.1
OG0010000
Physical Location & Seq
Forward (+)
8033 .. 12858
4826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009846.1_g000003.1.cds

Sequence Viewer

Length: 738 bp
atgtcatggctcgcccactcgctctctaaatccctccgaatcgacggcgacgacggcgaaggccaagatcacgacgacgacgtcgtaccccaccaccacaacaacaatgaagctgacgtcccgcccaccgcctccaccaaaccccaagacaacgaaatccaatcggatgcagaagcagttgaagacgcccaaacgcgtggcgtgaaagaagacctatcggaattccagcaaaccctgacgcgccaattctggggcgtcgcctcgtttcttgcgccaccaccgccctctgatcgattagcttctccacccgattgcgatcgatccgagccggccgatcacccgcccggtcaatcgccgatggcgttgaatgaagacgaagaagaggaggaggaggaggaggagtgcgccgtcggtgtcactgacgaggtcttggccttcgctagaaacatcgcgatgcatccagagacctggttggactttcctcttgatgaagaagaagacttgggagtacttagtgggagcataagaagaggtactctggcgaggcaatcagtggcagtcggggcaggtgaggaacaatatgagggttttacggcggcggaggtggatgacggggctggcagatgggctgtacatagggcttcaggtgatgacggggcattgagtggcgaggagcttgagtttttgcaaggtagccatatggatctagctgagttcaatgtggttgtagtcaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

245

Amino Acids

26.67

Weight (kDa)

4.13

Isoelectric Point (pI)

66.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 557
AasI GACNNNNNNGTC 1 cut(s) 80
AatII GACGTC 2 cut(s) 84, 120
Acc36I ACCTGC 1 cut(s) 557
AccII CGCG 3 cut(s) 196, 241, 452
AciI CCGC 6 cut(s) 122, 129, 281, 341, 596, 599
AclWI GGATC 2 cut(s) 315, 711
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 1 cut(s) 627
AcyI GRCGYC 4 cut(s) 81, 117, 186, 255
AfaI GTAC 4 cut(s) 87, 510, 535, 633
AfiI CCNNNNNNNGG 1 cut(s) 250
AflIII ACRYGT 1 cut(s) 194
AgsI TTSAA 3 cut(s) 182, 367, 718
AjnI CCWGG 1 cut(s) 467
AluBI AGCT 4 cut(s) 113, 299, 676, 710
AluI AGCT 4 cut(s) 113, 299, 676, 710
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 2 cut(s) 315, 711
AoxI GGCC 3 cut(s) 61, 330, 432
ApoI RAATTY 1 cut(s) 221
AspLEI GCGC 3 cut(s) 243, 274, 407
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 3 cut(s) 329, 581, 659
BbsI GAAGAC 4 cut(s) 189, 216, 378, 504
BccI CCATC 2 cut(s) 352, 618
BceAI ACGGC 4 cut(s) 61, 70, 392, 609
BciT130I CCWGG 1 cut(s) 469
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 2 cut(s) 441, 707
BfuAI ACCTGC 1 cut(s) 557
BisI GCNGC 1 cut(s) 597
BlsI GCNGC 1 cut(s) 598
BmcAI AGTACT 1 cut(s) 510
Bme1390I CCNGG 2 cut(s) 345, 469
BmrFI CCNGG 2 cut(s) 345, 469
BmsI GCATC 3 cut(s) 157, 444, 466
BpiI GAAGAC 4 cut(s) 189, 216, 378, 504
BpuEI CTTGAG 1 cut(s) 698
BpuMI CCSGG 1 cut(s) 345
Bsa29I ATCGAT 2 cut(s) 292, 319
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 4 cut(s) 81, 117, 186, 255
BsaI GGTCTC 1 cut(s) 458
BsaXI ACNNNNNCTCC 4 cut(s) 386, 416, 665, 695
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse118I RCCGGY 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 469
BseCI ATCGAT 2 cut(s) 292, 319
BseGI GGATG 3 cut(s) 172, 457, 613
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMII CTCAG 1 cut(s) 702
BseRI GAGGAG 7 cut(s) 398, 401, 404, 407, 410, 413, 686
BseX3I CGGCCG 1 cut(s) 330
Bsh1236I CGCG 3 cut(s) 196, 241, 452
Bsh1285I CGRYCG 2 cut(s) 319, 333
BshFI GGCC 3 cut(s) 63, 332, 434
BshVI ATCGAT 2 cut(s) 292, 319
BsiEI CGRYCG 2 cut(s) 319, 333
BsiSI CCGG 2 cut(s) 329, 345
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 3 cut(s) 63, 332, 434
Bso31I GGTCTC 1 cut(s) 458
Bsp1407I TGTACA 1 cut(s) 631
Bsp143I GATC 6 cut(s) 67, 289, 316, 320, 334, 703
Bsp68I TCGCGA 1 cut(s) 452
BspACI CCGC 6 cut(s) 122, 129, 281, 341, 596, 599
BspANI GGCC 3 cut(s) 63, 332, 434
BspCNI CTCAG 1 cut(s) 703
BspDI ATCGAT 2 cut(s) 292, 319
BspFNI CGCG 3 cut(s) 196, 241, 452
BspMI ACCTGC 1 cut(s) 557
BspPI GGATC 2 cut(s) 315, 711
BspTNI GGTCTC 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 631
BssAI RCCGGY 1 cut(s) 328
BssMI GATC 6 cut(s) 67, 289, 316, 320, 334, 703
BssNI GRCGYC 4 cut(s) 81, 117, 186, 255
Bst2UI CCWGG 1 cut(s) 469
Bst6I CTCTTC 2 cut(s) 375, 523
BstACI GRCGYC 4 cut(s) 81, 117, 186, 255
BstAUI TGTACA 1 cut(s) 631
BstC8I GCNNGC 3 cut(s) 12, 330, 619
BstDEI CTNAG 2 cut(s) 512, 711
BstF5I GGATG 3 cut(s) 172, 457, 613
BstFNI CGCG 3 cut(s) 196, 241, 452
BstHHI GCGC 3 cut(s) 243, 274, 407
BstKTI GATC 6 cut(s) 70, 292, 319, 323, 337, 706
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 6 cut(s) 67, 289, 316, 320, 334, 703
BstMCI CGRYCG 2 cut(s) 319, 333
BstMWI GCNNNNNNNGC 3 cut(s) 54, 280, 563
BstNI CCWGG 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 343, 467
BstUI CGCG 3 cut(s) 196, 241, 452
BstV2I GAAGAC 4 cut(s) 189, 216, 378, 504
BstX2I RGATCY 1 cut(s) 703
BstXI CCANNNNNNTGG 2 cut(s) 197, 468
BstYI RGATCY 1 cut(s) 703
BstZI CGGCCG 1 cut(s) 330
Bsu15I ATCGAT 2 cut(s) 292, 319
BsuRI GGCC 3 cut(s) 63, 332, 434
BsuTUI ATCGAT 2 cut(s) 292, 319
BtgZI GCGATG 2 cut(s) 433, 467
BtsCI GGATG 3 cut(s) 172, 457, 613
BtsIMutI CAGTG 2 cut(s) 417, 558
BtuMI TCGCGA 1 cut(s) 452
BveI ACCTGC 1 cut(s) 557
Cac8I GCNNGC 3 cut(s) 12, 330, 619
CfoI GCGC 3 cut(s) 243, 274, 407
Cfr10I RCCGGY 1 cut(s) 328
ClaI ATCGAT 2 cut(s) 292, 319
CseI GACGC 3 cut(s) 194, 244, 247
CsiI ACCWGGT 1 cut(s) 467
Csp6I GTAC 4 cut(s) 86, 509, 534, 632
CviAII CATG 1 cut(s) 6
CviQI GTAC 4 cut(s) 86, 509, 534, 632
DdeI CTNAG 2 cut(s) 512, 711
DpnI GATC 6 cut(s) 69, 291, 318, 322, 336, 705
DpnII GATC 6 cut(s) 67, 289, 316, 320, 334, 703
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 330
EagI CGGCCG 1 cut(s) 330
Eam1104I CTCTTC 2 cut(s) 375, 523
EarI CTCTTC 2 cut(s) 375, 523
EciI GGCGGA 1 cut(s) 614
EclXI CGGCCG 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 458
Eco52I CGGCCG 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 627
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 467
EcoT22I ATGCAT 1 cut(s) 459
FaeI CATG 1 cut(s) 9
FaiI YATR 6 cut(s) 7, 524, 582, 636, 699, 701
FaqI GGGAC 1 cut(s) 104
FatI CATG 1 cut(s) 5
FauI CCCGC 2 cut(s) 129, 348
FauNDI CATATG 1 cut(s) 699
Fnu4HI GCNGC 1 cut(s) 597
FokI GGATG 3 cut(s) 179, 444, 620
Fsp4HI GCNGC 1 cut(s) 597
FspBI CTAG 2 cut(s) 441, 707
GlaI GCGC 3 cut(s) 242, 273, 406
GluI GCNGC 1 cut(s) 597
HaeIII GGCC 3 cut(s) 63, 332, 434
HapII CCGG 2 cut(s) 329, 345
HgaI GACGC 3 cut(s) 194, 244, 247
HhaI GCGC 3 cut(s) 243, 274, 407
Hin1I GRCGYC 4 cut(s) 81, 117, 186, 255
Hin1II CATG 1 cut(s) 9
Hin6I GCGC 3 cut(s) 241, 272, 405
HinP1I GCGC 3 cut(s) 241, 272, 405
HinfI GANTC 1 cut(s) 39
HpaII CCGG 2 cut(s) 329, 345
HphI GGTGA 3 cut(s) 329, 581, 659
Hpy188I TCNGA 5 cut(s) 38, 166, 220, 289, 325
Hpy188III TCNNGA 4 cut(s) 71, 451, 461, 485
Hpy99I CGWCG 9 cut(s) 47, 53, 56, 77, 80, 83, 86, 260, 413
HpyAV CCTTC 2 cut(s) 53, 445
HpyCH4IV ACGT 2 cut(s) 81, 117
HpyCH4V TGCA 3 cut(s) 170, 457, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 280, 563
HpyF3I CTNAG 2 cut(s) 512, 711
HpySE526I ACGT 2 cut(s) 81, 117
Hsp92I GRCGYC 4 cut(s) 81, 117, 186, 255
Hsp92II CATG 1 cut(s) 9
HspAI GCGC 3 cut(s) 241, 272, 405
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Kzo9I GATC 6 cut(s) 67, 289, 316, 320, 334, 703
LmnI GCTCC 2 cut(s) 519, 673
LweI GCATC 3 cut(s) 157, 444, 466
MabI ACCWGGT 1 cut(s) 467
MaeI CTAG 2 cut(s) 441, 707
MaeII ACGT 2 cut(s) 81, 117
MaeIII GTNAC 1 cut(s) 415
MalI GATC 6 cut(s) 69, 291, 318, 322, 336, 705
MboI GATC 6 cut(s) 67, 289, 316, 320, 334, 703
MboII GAAGA 9 cut(s) 194, 221, 383, 389, 392, 503, 506, 509, 540
MflI RGATCY 1 cut(s) 703
MluCI AATT 2 cut(s) 221, 245
MluI ACGCGT 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 453
Mph1103I ATGCAT 1 cut(s) 459
MroNI GCCGGC 1 cut(s) 328
MslI CAYNNNNRTG 1 cut(s) 452
MspI CCGG 2 cut(s) 329, 345
MspR9I CCNGG 2 cut(s) 345, 469
MvaI CCWGG 1 cut(s) 469
MvnI CGCG 3 cut(s) 196, 241, 452
MwoI GCNNNNNNNGC 3 cut(s) 54, 280, 563
NaeI GCCGGC 1 cut(s) 330
NciI CCSGG 1 cut(s) 345
NdeI CATATG 1 cut(s) 699
NdeII GATC 6 cut(s) 67, 289, 316, 320, 334, 703
NgoMIV GCCGGC 1 cut(s) 328
NlaIII CATG 1 cut(s) 9
NmuCI GTSAC 1 cut(s) 415
NruI TCGCGA 1 cut(s) 452
NsiI ATGCAT 1 cut(s) 459
PaqCI CACCTGC 1 cut(s) 557
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 78, 81, 359
PdiI GCCGGC 1 cut(s) 330
PfeI GAWTC 1 cut(s) 39
PflFI GACNNNGTC 2 cut(s) 80, 425
PkrI GCNGC 1 cut(s) 598
Ple19I CGATCG 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PsuI RGATCY 1 cut(s) 703
PsyI GACNNNGTC 2 cut(s) 80, 425
PvuI CGATCG 1 cut(s) 319
RruI TCGCGA 1 cut(s) 452
RsaI GTAC 4 cut(s) 87, 510, 535, 633
RsaNI GTAC 4 cut(s) 86, 509, 534, 632
RseI CAYNNNNRTG 1 cut(s) 452
SatI GCNGC 1 cut(s) 597
Sau3AI GATC 6 cut(s) 67, 289, 316, 320, 334, 703
ScaI AGTACT 1 cut(s) 510
ScrFI CCNGG 2 cut(s) 345, 469
SexAI ACCWGGT 1 cut(s) 467
SfaNI GCATC 3 cut(s) 157, 444, 466
SmiMI CAYNNNNRTG 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 2 cut(s) 221, 245
SsiI CCGC 6 cut(s) 122, 129, 281, 341, 596, 599
SspMI CTAG 2 cut(s) 441, 707
StyD4I CCNGG 2 cut(s) 343, 467
TaiI ACGT 2 cut(s) 84, 120
TaqI TCGA 3 cut(s) 42, 292, 319
TasI AATT 2 cut(s) 221, 245
TatI WGTACW 2 cut(s) 508, 631
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 1 cut(s) 39
TscAI CASTG 2 cut(s) 424, 558
TseFI GTSAC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 3 cut(s) 123, 384, 504
TspRI CASTG 2 cut(s) 424, 558
Tth111I GACNNNGTC 2 cut(s) 80, 425
XapI RAATTY 1 cut(s) 221
XspI CTAG 2 cut(s) 441, 707
ZraI GACGTC 2 cut(s) 82, 118
ZrmI AGTACT 1 cut(s) 510
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.