Rh6CG503800

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0010000
Physical Location & Seq
Forward (+)
66604611 .. 66605845
1235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG503800.1

Sequence Viewer

Length: 852 bp
ATGTCATGGCTCGCCCACTCGCTCTCTAAATCCCTCCGTATCGACGGCGACGGCGAAGGCCAAGATCACGACGACGACGTCGTACCCCACCGCAACACCAACAATGAAGCTGACGTCCCGCCCACCGCCTCCACCAAACCCCAAGACAAAGACAACAACGAAATCCAATCGGATGCAGAAGCAGTTGAAGACGCCCAAACGCGTGGCGTGAAAGAAGACCTATCGGAATTCCAGCAAACCCTAACGCGCCAATTCTGGGGCGTCGCCTCGTTCCTTGCGCCACCACCGCCCTCTGATCGATTAGTTTCTCCGCCCGATTGCGATCGATCCGAGCCGGCCGATCACCCACCCGGTCAATCGCCGATGGCGTTCAATGAAGGTGAAGAAGAAGAGGAGGAGGAGGAGTGCGCCGTCGGTGTCACTGACGAGGTCTTGGCCTTCGCTAGAAACATCGCGATGCATCCAGAGACCTGGTTGGACTTTCCTCTTGATGAAGAAGAAGACTTGGGAGATTTTGAAATGTCTGATGCCCAGCAAGAGCATGCTATGATGATTGAACATCTTGCGCCAAGATTAGCTGCTTTGAGAATCGAGCTTTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAACAAGCAGGATGCTGAGGTTTTGTCCACACCTCTGGTAAAGCTTTTCGTTTTTGTAATTGCTTTCAGTGTCCCTTTATCAAAGGATTCAATTCAATGCTGGTTCAAAATTAGACCTCAAAACTTTGAGTTTAAGATACTACTATGTAATATATCAAGGTGCAAACTGTTGCTTCATGCCAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

283

Amino Acids

32.03

Weight (kDa)

4.49

Isoelectric Point (pI)

65.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 170 - 225 9.5e-14 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 2 cut(s) 81, 117
AccII CGCG 3 cut(s) 202, 247, 455
AciI CCGC 5 cut(s) 91, 119, 126, 287, 311
AclWI GGATC 1 cut(s) 321
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 1 cut(s) 227
AcyI GRCGYC 4 cut(s) 78, 114, 192, 261
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 256
AflIII ACRYGT 1 cut(s) 200
AgsI TTSAA 7 cut(s) 188, 373, 518, 557, 753, 758, 769
AjnI CCWGG 1 cut(s) 470
AluBI AGCT 4 cut(s) 110, 578, 595, 706
AluI AGCT 4 cut(s) 110, 578, 595, 706
Alw26I GTCTC 1 cut(s) 461
AlwI GGATC 1 cut(s) 321
AoxI GGCC 3 cut(s) 58, 336, 435
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 1 cut(s) 227
AspLEI GCGC 4 cut(s) 249, 280, 410, 568
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 2 cut(s) 335, 392
BbsI GAAGAC 3 cut(s) 195, 222, 507
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 1 cut(s) 565
BccI CCATC 1 cut(s) 358
BceAI ACGGC 3 cut(s) 61, 67, 395
BciT130I CCWGG 1 cut(s) 472
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 1 cut(s) 444
BisI GCNGC 1 cut(s) 579
BlsI GCNGC 1 cut(s) 580
Bme1390I CCNGG 2 cut(s) 351, 472
BmrFI CCNGG 2 cut(s) 351, 472
BmsI GCATC 5 cut(s) 163, 447, 469, 517, 664
BpiI GAAGAC 3 cut(s) 195, 222, 507
Bpu10I CCTNAGC 1 cut(s) 678
BpuMI CCSGG 1 cut(s) 351
Bsa29I ATCGAT 2 cut(s) 298, 325
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 4 cut(s) 78, 114, 192, 261
BsaI GGTCTC 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse118I RCCGGY 1 cut(s) 334
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 1 cut(s) 472
BseCI ATCGAT 2 cut(s) 298, 325
BseGI GGATG 3 cut(s) 178, 460, 679
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 669
BseRI GAGGAG 4 cut(s) 407, 410, 413, 416
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 565
BseYI CCCAGC 1 cut(s) 531
Bsh1236I CGCG 3 cut(s) 202, 247, 455
Bsh1285I CGRYCG 2 cut(s) 325, 339
BshFI GGCC 3 cut(s) 60, 338, 437
BshVI ATCGAT 2 cut(s) 298, 325
BsiEI CGRYCG 2 cut(s) 325, 339
BsiSI CCGG 2 cut(s) 335, 351
BslFI GGGAC 2 cut(s) 101, 719
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 2 cut(s) 101, 719
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 3 cut(s) 60, 338, 437
Bso31I GGTCTC 1 cut(s) 461
Bsp143I GATC 5 cut(s) 64, 295, 322, 326, 340
Bsp68I TCGCGA 1 cut(s) 455
BspACI CCGC 5 cut(s) 91, 119, 126, 287, 311
BspANI GGCC 3 cut(s) 60, 338, 437
BspCNI CTCAG 1 cut(s) 670
BspDI ATCGAT 2 cut(s) 298, 325
BspFNI CGCG 3 cut(s) 202, 247, 455
BspPI GGATC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 461
BsrFI RCCGGY 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 334
BssMI GATC 5 cut(s) 64, 295, 322, 326, 340
BssNI GRCGYC 4 cut(s) 78, 114, 192, 261
Bst2UI CCWGG 1 cut(s) 472
Bst4CI ACNGT 1 cut(s) 831
Bst6I CTCTTC 1 cut(s) 384
BstACI GRCGYC 4 cut(s) 78, 114, 192, 261
BstC8I GCNNGC 3 cut(s) 12, 336, 543
BstDEI CTNAG 1 cut(s) 678
BstF5I GGATG 3 cut(s) 178, 460, 679
BstFNI CGCG 3 cut(s) 202, 247, 455
BstHHI GCGC 4 cut(s) 249, 280, 410, 568
BstKTI GATC 5 cut(s) 67, 298, 325, 329, 343
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 5 cut(s) 64, 295, 322, 326, 340
BstMCI CGRYCG 2 cut(s) 325, 339
BstMWI GCNNNNNNNGC 4 cut(s) 286, 612, 658, 667
BstNI CCWGG 1 cut(s) 472
BstNSI RCATGY 1 cut(s) 545
BstSCI CCNGG 2 cut(s) 349, 470
BstUI CGCG 3 cut(s) 202, 247, 455
BstV1I GCAGC 1 cut(s) 565
BstV2I GAAGAC 3 cut(s) 195, 222, 507
BstXI CCANNNNNNTGG 3 cut(s) 203, 471, 697
BstZI CGGCCG 1 cut(s) 336
Bsu15I ATCGAT 2 cut(s) 298, 325
BsuRI GGCC 3 cut(s) 60, 338, 437
BsuTUI ATCGAT 2 cut(s) 298, 325
BtgZI GCGATG 2 cut(s) 436, 470
BtsCI GGATG 3 cut(s) 178, 460, 679
BtsIMutI CAGTG 2 cut(s) 420, 736
BtuMI TCGCGA 1 cut(s) 455
Cac8I GCNNGC 3 cut(s) 12, 336, 543
CfoI GCGC 4 cut(s) 249, 280, 410, 568
Cfr10I RCCGGY 1 cut(s) 334
ClaI ATCGAT 2 cut(s) 298, 325
CseI GACGC 2 cut(s) 200, 250
CsiI ACCWGGT 1 cut(s) 470
Csp6I GTAC 1 cut(s) 83
CviAII CATG 3 cut(s) 6, 542, 839
CviQI GTAC 1 cut(s) 83
DdeI CTNAG 1 cut(s) 678
DpnI GATC 5 cut(s) 66, 297, 324, 328, 342
DpnII GATC 5 cut(s) 64, 295, 322, 326, 340
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 336
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
EciI GGCGGA 1 cut(s) 300
EclXI CGGCCG 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 461
Eco52I CGGCCG 1 cut(s) 336
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 470
EcoT22I ATGCAT 1 cut(s) 462
FaeI CATG 3 cut(s) 9, 545, 842
FaiI YATR 8 cut(s) 7, 543, 548, 609, 611, 808, 815, 840
FaqI GGGAC 2 cut(s) 101, 719
FatI CATG 3 cut(s) 5, 541, 838
FauI CCCGC 1 cut(s) 126
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 1 cut(s) 579
FokI GGATG 3 cut(s) 185, 447, 686
Fsp4HI GCNGC 1 cut(s) 579
FspBI CTAG 1 cut(s) 444
GlaI GCGC 4 cut(s) 248, 279, 409, 567
GluI GCNGC 1 cut(s) 579
GsaI CCCAGC 1 cut(s) 535
HaeIII GGCC 3 cut(s) 60, 338, 437
HapII CCGG 2 cut(s) 335, 351
HgaI GACGC 2 cut(s) 200, 250
HhaI GCGC 4 cut(s) 249, 280, 410, 568
Hin1I GRCGYC 4 cut(s) 78, 114, 192, 261
Hin1II CATG 3 cut(s) 9, 545, 842
Hin6I GCGC 4 cut(s) 247, 278, 408, 566
HinP1I GCGC 4 cut(s) 247, 278, 408, 566
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 2 cut(s) 588, 749
HpaII CCGG 2 cut(s) 335, 351
HphI GGTGA 2 cut(s) 335, 392
Hpy166II GTNNAC 1 cut(s) 690
Hpy188I TCNGA 5 cut(s) 172, 226, 295, 331, 526
Hpy188III TCNNGA 5 cut(s) 68, 454, 464, 488, 628
Hpy8I GTNNAC 1 cut(s) 690
Hpy99I CGWCG 8 cut(s) 47, 53, 74, 77, 80, 83, 266, 416
HpyAV CCTTC 3 cut(s) 50, 371, 448
HpyCH4III ACNGT 1 cut(s) 831
HpyCH4IV ACGT 2 cut(s) 78, 114
HpyCH4V TGCA 4 cut(s) 176, 460, 652, 825
HpyF10VI GCNNNNNNNGC 4 cut(s) 286, 612, 658, 667
HpyF3I CTNAG 1 cut(s) 678
HpySE526I ACGT 2 cut(s) 78, 114
Hsp92I GRCGYC 4 cut(s) 78, 114, 192, 261
Hsp92II CATG 3 cut(s) 9, 545, 842
HspAI GCGC 4 cut(s) 247, 278, 408, 566
KroI GCCGGC 1 cut(s) 334
KroNI GCCGGC 1 cut(s) 336
Kzo9I GATC 5 cut(s) 64, 295, 322, 326, 340
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 5 cut(s) 163, 447, 469, 517, 664
MabI ACCWGGT 1 cut(s) 470
MaeI CTAG 1 cut(s) 444
MaeII ACGT 2 cut(s) 78, 114
MaeIII GTNAC 2 cut(s) 418, 620
MalI GATC 5 cut(s) 66, 297, 324, 328, 342
MboI GATC 5 cut(s) 64, 295, 322, 326, 340
MboII GAAGA 9 cut(s) 200, 227, 395, 398, 401, 506, 509, 512, 638
MluCI AATT 5 cut(s) 227, 251, 720, 753, 771
MluI ACGCGT 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 456
Mph1103I ATGCAT 1 cut(s) 462
MroNI GCCGGC 1 cut(s) 334
MseI TTAA 1 cut(s) 795
MslI CAYNNNNRTG 1 cut(s) 455
MspI CCGG 2 cut(s) 335, 351
MspR9I CCNGG 2 cut(s) 351, 472
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 1 cut(s) 472
MvnI CGCG 3 cut(s) 202, 247, 455
MwoI GCNNNNNNNGC 4 cut(s) 286, 612, 658, 667
NaeI GCCGGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 351
NdeI CATATG 1 cut(s) 609
NdeII GATC 5 cut(s) 64, 295, 322, 326, 340
NgoMIV GCCGGC 1 cut(s) 334
NlaIII CATG 3 cut(s) 9, 545, 842
NmuCI GTSAC 1 cut(s) 418
NruI TCGCGA 1 cut(s) 455
NsiI ATGCAT 1 cut(s) 462
NspI RCATGY 1 cut(s) 545
PaeI GCATGC 1 cut(s) 545
PcsI WCGNNNNNNNCGW 4 cut(s) 51, 75, 78, 365
PctI GAATGC 1 cut(s) 652
PdiI GCCGGC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 588, 749
PflFI GACNNNGTC 2 cut(s) 77, 428
PkrI GCNGC 1 cut(s) 580
Ple19I CGATCG 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 470
PspFI CCCAGC 1 cut(s) 531
PspGI CCWGG 1 cut(s) 470
PsyI GACNNNGTC 2 cut(s) 77, 428
PvuI CGATCG 1 cut(s) 325
RruI TCGCGA 1 cut(s) 455
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 1 cut(s) 795
SatI GCNGC 1 cut(s) 579
Sau3AI GATC 5 cut(s) 64, 295, 322, 326, 340
ScrFI CCNGG 2 cut(s) 351, 472
SexAI ACCWGGT 1 cut(s) 470
SfaNI GCATC 5 cut(s) 163, 447, 469, 517, 664
SmiMI CAYNNNNRTG 1 cut(s) 455
SphI GCATGC 1 cut(s) 545
Sse9I AATT 5 cut(s) 227, 251, 720, 753, 771
SsiI CCGC 5 cut(s) 91, 119, 126, 287, 311
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 349, 470
TaaI ACNGT 1 cut(s) 831
TaiI ACGT 2 cut(s) 81, 117
TaqI TCGA 5 cut(s) 42, 298, 325, 591, 656
TasI AATT 5 cut(s) 227, 251, 720, 753, 771
TfiI GAWTC 2 cut(s) 588, 749
Tru1I TTAA 1 cut(s) 795
Tru9I TTAA 1 cut(s) 795
TscAI CASTG 2 cut(s) 427, 736
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 1 cut(s) 578
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 4 cut(s) 120, 390, 507, 827
TspGWI ACGGA 2 cut(s) 26, 591
TspRI CASTG 2 cut(s) 427, 736
Tth111I GACNNNGTC 2 cut(s) 77, 428
XapI RAATTY 1 cut(s) 227
XceI RCATGY 1 cut(s) 545
XspI CTAG 1 cut(s) 444
ZraI GACGTC 2 cut(s) 79, 115
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.