MD08G1079400.v1.1

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010000
Physical Location & Seq
Reverse (-)
6603438 .. 6610371
6934 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1079400.v1.1.491

Sequence Viewer

Length: 798 bp
ATGAACTTGGCATTCACCAAGAACATCGCGATGCATCCGGAGACGTGGCTGGACTTTCCTCTGGACGAGGAGGAGGATTTGGGCGATTTTGACATGTCTGATGCCCAGCAAGAGCATGCTATGGTGATTGAACATCTTGCGCCAAGATTTGCTGCTTTGAGATTTGAACTGTGTCCGTGCCATATGAGCGAGGGTTACTTCTGGAAAGTCTACTTTGTTCTTCTGCATTCGAGGCTCAATAAGCAGGATGCCGAGGTCTTATCCACACCCCAAATAATGGAAGCCAGAGCAAAGTGGATGCAGGAGCTACATTGTCAGACTGCATTGGAACATGATCAATTTGGAAGAAACACTTCTTTTTTAAAAGACAGTTCCAATATACTAGATGACGATTGTGCTCCGGTCTCATCATGTAGTGTTTATTACGAGGACAAGATGCTAAGGACGTATCCTTATGAATCAACGATGTCCCCCACGGCTTATGAGACTGAGAAGCACCCAGTTGAGAGTGATGAAGTCCCATTTATTGACAAGTCTGTTATTGAAGAAAAGCCCATCATTAAGAGCGAGGACAAGAATCTAACAGTTGGTCCATCCTCGAAGATGCTAGATAATATCTACGAAGATGATGACGATGATGACTGGCCAGAGGAAGATTCTGAATTAGATGGGTATAGTGTAACGGCTATTCACGCAATCAATGAAGAAGACATATCTTTCAGTGACCTAGAGGACGATGATTTCCTTGCTCCCACAAAAATGAAGACTGTCAAGAAACCTCGAGGAAAGAGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

266

Amino Acids

30.56

Weight (kDa)

4.4

Isoelectric Point (pI)

63.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 28 - 83 1.8e-12 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 780
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 1 cut(s) 210
AccII CGCG 1 cut(s) 29
AccIII TCCGGA 1 cut(s) 37
AcoI YGGCCR 1 cut(s) 644
AfiI CCNNNNNNNGG 1 cut(s) 277
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 3 cut(s) 131, 167, 545
AjiI CACGTC 1 cut(s) 45
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 3 cut(s) 35, 409, 479
Ama87I CYCGRG 1 cut(s) 780
Aor13HI TCCGGA 1 cut(s) 37
AoxI GGCC 1 cut(s) 644
ApeKI GCWGC 1 cut(s) 152
Asp700I GAANNNNTTC 1 cut(s) 352
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 590
AsuHPI GGTGA 2 cut(s) 7, 136
AvaI CYCGRG 1 cut(s) 780
AvaII GGWCC 1 cut(s) 590
BalI TGGCCA 1 cut(s) 646
BbsI GAAGAC 2 cut(s) 714, 770
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 1 cut(s) 139
BccI CCATC 3 cut(s) 563, 601, 662
BceAI ACGGC 2 cut(s) 492, 699
BciVI GTATCC 1 cut(s) 459
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 3 cut(s) 35, 409, 479
BfaI CTAG 3 cut(s) 383, 608, 728
BfuI GTATCC 1 cut(s) 459
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 1 cut(s) 780
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 590
BmrI ACTGGG 1 cut(s) 494
BmsI GCATC 7 cut(s) 21, 43, 91, 238, 288, 426, 594
BmuI ACTGGG 1 cut(s) 494
BpiI GAAGAC 2 cut(s) 714, 770
Bpu10I CCTNAGC 1 cut(s) 440
BsaI GGTCTC 1 cut(s) 409
BsaJI CCNNGG 2 cut(s) 252, 474
BsaWI WCCGGW 2 cut(s) 37, 400
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 500, 647
BseAI TCCGGA 1 cut(s) 37
BseDI CCNNGG 2 cut(s) 252, 474
BseGI GGATG 4 cut(s) 34, 253, 303, 593
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 2 cut(s) 500, 647
BseRI GAGGAG 2 cut(s) 83, 86
BseXI GCAGC 1 cut(s) 139
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 646
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 780
BsiSI CCGG 2 cut(s) 38, 401
BslFI GGGAC 2 cut(s) 454, 503
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 3 cut(s) 35, 409, 479
BsmBI CGTCTC 1 cut(s) 35
BsmFI GGGAC 2 cut(s) 454, 503
BsmI GAATGC 2 cut(s) 11, 226
BsnI GGCC 1 cut(s) 646
Bso31I GGTCTC 1 cut(s) 409
BsoBI CYCGRG 1 cut(s) 780
Bsp1286I GDGCHC 1 cut(s) 400
Bsp13I TCCGGA 1 cut(s) 37
Bsp143I GATC 1 cut(s) 334
Bsp68I TCGCGA 1 cut(s) 29
BspANI GGCC 1 cut(s) 646
BspCNI CTCAG 1 cut(s) 481
BspEI TCCGGA 1 cut(s) 37
BspFNI CGCG 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 409
BsrI ACTGG 2 cut(s) 500, 647
BssECI CCNNGG 2 cut(s) 252, 474
BssMI GATC 1 cut(s) 334
Bst4CI ACNGT 4 cut(s) 171, 371, 586, 769
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 2 cut(s) 440, 489
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 4 cut(s) 34, 253, 303, 593
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 3 cut(s) 35, 409, 479
BstMBI GATC 1 cut(s) 334
BstMWI GCNNNNNNNGC 4 cut(s) 186, 232, 241, 692
BstNSI RCATGY 2 cut(s) 97, 119
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 2 cut(s) 714, 770
BsuI GTATCC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 646
BtgI CCRYGG 1 cut(s) 474
BtgZI GCGATG 2 cut(s) 10, 44
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 4 cut(s) 34, 253, 303, 593
BtsIMutI CAGTG 1 cut(s) 727
BtuMI TCGCGA 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 117
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 590
CviAII CATG 4 cut(s) 94, 116, 332, 411
CviJI RGCY 9 cut(s) 49, 235, 284, 307, 479, 553, 646, 686, 795
CviKI_1 RGCY 9 cut(s) 49, 235, 284, 307, 479, 553, 646, 686, 795
DdeI CTNAG 2 cut(s) 440, 489
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
DraI TTTAAA 1 cut(s) 363
EaeI YGGCCR 1 cut(s) 644
Eco31I GGTCTC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 780
EcoT22I ATGCAT 1 cut(s) 36
Esp3I CGTCTC 1 cut(s) 35
FaeI CATG 4 cut(s) 97, 119, 335, 414
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FaqI GGGAC 2 cut(s) 454, 503
FatI CATG 4 cut(s) 93, 115, 331, 410
FauNDI CATATG 1 cut(s) 183
FbaI TGATCA 1 cut(s) 334
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 4 cut(s) 21, 260, 310, 580
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 3 cut(s) 383, 608, 728
GlaI GCGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 153
GsaI CCCAGC 1 cut(s) 109
HaeIII GGCC 1 cut(s) 646
HapII CCGG 2 cut(s) 38, 401
HhaI GCGC 1 cut(s) 142
Hin1II CATG 4 cut(s) 97, 119, 335, 414
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HinfI GANTC 3 cut(s) 458, 577, 656
HpaII CCGG 2 cut(s) 38, 401
HphI GGTGA 2 cut(s) 7, 136
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 3 cut(s) 100, 318, 661
Hpy188III TCNNGA 5 cut(s) 28, 38, 62, 202, 772
Hpy8I GTNNAC 1 cut(s) 211
HpyCH4III ACNGT 4 cut(s) 171, 371, 586, 769
HpyCH4IV ACGT 2 cut(s) 44, 446
HpyCH4V TGCA 4 cut(s) 34, 226, 301, 323
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 232, 241, 692
HpyF3I CTNAG 2 cut(s) 440, 489
HpySE526I ACGT 2 cut(s) 44, 446
Hsp92II CATG 4 cut(s) 97, 119, 335, 414
HspAI GCGC 1 cut(s) 140
Kpn2I TCCGGA 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 1 cut(s) 334
LmnI GCTCC 3 cut(s) 304, 403, 754
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 7 cut(s) 21, 43, 91, 238, 288, 426, 594
MaeI CTAG 3 cut(s) 383, 608, 728
MaeII ACGT 2 cut(s) 44, 446
MaeIII GTNAC 3 cut(s) 194, 679, 722
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 9 cut(s) 212, 357, 557, 613, 635, 665, 716, 719, 775
MhlI GDGCHC 1 cut(s) 400
MlsI TGGCCA 1 cut(s) 646
MluCI AATT 2 cut(s) 338, 662
MluNI TGGCCA 1 cut(s) 646
Mox20I TGGCCA 1 cut(s) 646
Mph1103I ATGCAT 1 cut(s) 36
MroI TCCGGA 1 cut(s) 37
MroXI GAANNNNTTC 1 cut(s) 352
MscI TGGCCA 1 cut(s) 646
MseI TTAA 2 cut(s) 362, 561
MslI CAYNNNNRTG 2 cut(s) 29, 758
Msp20I TGGCCA 1 cut(s) 646
MspI CCGG 2 cut(s) 38, 401
Mva1269I GAATGC 2 cut(s) 11, 226
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 4 cut(s) 186, 232, 241, 692
NdeI CATATG 1 cut(s) 183
NdeII GATC 1 cut(s) 334
NlaIII CATG 4 cut(s) 97, 119, 335, 414
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 722
NruI TCGCGA 1 cut(s) 29
NsiI ATGCAT 1 cut(s) 36
NspI RCATGY 2 cut(s) 97, 119
PaeI GCATGC 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 780
PciI ACATGT 1 cut(s) 93
PctI GAATGC 2 cut(s) 11, 226
PdmI GAANNNNTTC 1 cut(s) 352
PfeI GAWTC 3 cut(s) 458, 577, 656
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 1 cut(s) 154
PscI ACATGT 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 105
PspPI GGNCC 1 cut(s) 590
PspXI VCTCGAGB 1 cut(s) 780
RruI TCGCGA 1 cut(s) 29
RseI CAYNNNNRTG 2 cut(s) 29, 758
SaqAI TTAA 2 cut(s) 362, 561
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 1 cut(s) 334
Sau96I GGNCC 1 cut(s) 590
SduI GDGCHC 1 cut(s) 400
SetI ASST 6 cut(s) 47, 258, 309, 449, 729, 781
SfaNI GCATC 7 cut(s) 21, 43, 91, 238, 288, 426, 594
Sfr274I CTCGAG 1 cut(s) 780
SinI GGWCC 1 cut(s) 590
SlaI CTCGAG 1 cut(s) 780
SmiMI CAYNNNNRTG 2 cut(s) 29, 758
SmlI CTYRAG 1 cut(s) 780
SmoI CTYRAG 1 cut(s) 780
SphI GCATGC 1 cut(s) 119
Sse9I AATT 2 cut(s) 338, 662
SspMI CTAG 3 cut(s) 383, 608, 728
TaaI ACNGT 4 cut(s) 171, 371, 586, 769
TaiI ACGT 2 cut(s) 47, 449
TaqI TCGA 3 cut(s) 230, 599, 781
TasI AATT 2 cut(s) 338, 662
TfiI GAWTC 3 cut(s) 458, 577, 656
Tru1I TTAA 2 cut(s) 362, 561
Tru9I TTAA 2 cut(s) 362, 561
TscAI CASTG 1 cut(s) 727
TseFI GTSAC 1 cut(s) 722
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 1 cut(s) 722
TspDTI ATGAA 5 cut(s) 17, 471, 528, 717, 776
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 727
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 590
XceI RCATGY 2 cut(s) 97, 119
XhoI CTCGAG 1 cut(s) 780
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 352
XspI CTAG 3 cut(s) 383, 608, 728
Zsp2I ATGCAT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.